CN103535190A - Novel inoculating shovel for edible mushroom parent strain expansion breeding - Google Patents

Novel inoculating shovel for edible mushroom parent strain expansion breeding Download PDF

Info

Publication number
CN103535190A
CN103535190A CN201310512706.1A CN201310512706A CN103535190A CN 103535190 A CN103535190 A CN 103535190A CN 201310512706 A CN201310512706 A CN 201310512706A CN 103535190 A CN103535190 A CN 103535190A
Authority
CN
China
Prior art keywords
shovel
inoculation
edible mushroom
parent strain
novel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310512706.1A
Other languages
Chinese (zh)
Inventor
李慧敏
陆晓民
高青海
苏向宇
袁奎
王铭吉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anhui University of Science and Technology
Original Assignee
Anhui University of Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anhui University of Science and Technology filed Critical Anhui University of Science and Technology
Priority to CN201310512706.1A priority Critical patent/CN103535190A/en
Publication of CN103535190A publication Critical patent/CN103535190A/en
Pending legal-status Critical Current

Links

Images

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a novel inoculating shovel for edible mushroom parent strain expansion breeding and belongs to the technical field of edible mushroom inoculation. The inoculating shovel comprises a handle, a rod and a shovel body. The inoculating shovel is simple to operate, fast, efficient, low in inoculation pollution rate, good in inoculation effect, suitable for edible mushroom parent strain expansion breeding or related microorganism teaching and research, and the like.

Description

A kind of novel inoculation shovel that expands breeding that turns for parent edible fungus kind
Technical field
The present invention relates to a kind of inoculation shovel for the switching of parent edible fungus kind, belong to technical field of edible fungi inoculation.
Background technology
Edible fungus species, be edible mushroom good " seed ", for breeding, cultivate the pure mycelium fragment of edible mushroom of fruit body of edible fungi, it is just as the seed of crops, it is the lifeline of Edible Fungi, its quality can directly have influence on output and the quality of edible mushroom, and obtaining good bacterial classification is to obtain highly efficient and productive key point.Therefore the production of hybrid seeds of edible mushroom is an extremely important technical work in producing.
Edible fungus species is many by artificial culture, and its bacterial classification is pressed the difference in cultivation stage and source, can denominator kind, three kinds of original seed and cultivated speciess.From the spore separation of edible mushroom, cultivate or organize separated cultivation, substrate mycelium separation to cultivate resulting first generation mycelium, be called female plant (being also test tube kind, one-level kind), more very thin because of it, a little less than decomposing the ability of nutriment, normal adopt to be rich on the agar medium that nutrition is easy to again absorb cultivate, and female kind before switching is cultivated as original seed, how need turn and expand for several times at test tube or female agar medium of planting flat-plate special blake bottle, to improve breeding amount, purification and rejuvenation or for provenance preservation etc.
Female kind is the mother of bacterial classification productions, and it expands numerous is a technical stronger job, require provenance single pure, medium sterilization is thorough, equipment disinfection is comprehensive, and operates in relatively aseptic environment, has allowed anything but miscellaneous bacteria infection.Currently for parent edible fungus kind, turn the main inoculating tool that expands breeding and have inoculation hoe (inoculation rake) and inoculation to shovel (vaccinating lancet) two kinds of instruments, the inoculation hoe of current use (inoculation rake) be a kind of front end with crotch like the same instrument of hoe (rake), (vaccinating lancet) shoveled in inoculation is that a kind of front end is with the cutlery of single-blade flat mouth.In parent edible fungus kind, turn in expansion breeding and need the two to be used in conjunction with, first with inoculation hoe (inoculation rake), by it, long handle hoe (drag head) is goed deep in test tube or blake bottle first as bought bean curd medium to be carried out to horizontal cutting-up becoming some sections, then with inoculation shovel (vaccinating lancet), on the both sides of laterally drawing section medium, carry out after 2 longitudinal cutting-ups, from bottom, carry out again grafter, square body medium with mycelia is taken out, move on sterilized fresh culture.This method inoculation hoe, inoculation shovel all need through flame sterilization, and inoculation shovel (vaccinating lancet) enters former female kind and in pipe, need 3 cutting-ups and the easy landing of bacterium piece when taking out, and make the whole inoculation length that expends time in, and inoculation efficiency is low, has increased the infection chance of miscellaneous bacteria.How to utilize advanced inoculating tool, make inoculation operation faster, it is short that tube (bottle) opening time is tried one's best, and to reduce pollution rate, is the problem that is worth thinking.
Parent edible fungus kind of the present invention turns the inoculation shovel that expands breeding, with inoculation hoe, by certain width, carving after horizontal score, by " U " inoculation shovel mouthful upwards by certain depth forward horizontal sliding just easily once shovel out the square bacterial classification of a slice adhesion, and bacterium piece is difficult for landing when taking out, can save time, improve work efficiency, reduce and pollute.
Summary of the invention
The object of this invention is to provide a kind of design that turns the novel inoculation shovel of expansion breeding for parent edible fungus kind, so that promote its extensive use in parent edible fungus kind is produced or tested.
Technical scheme
The present invention is achieved in that
1. a Novel edible fungi inoculation shovel, consists of handle, the body of rod, shovel body three parts.2. the shovel body structure described in is " U " type, and there is the protruding edge of shovel on each limit of front end.The material using is stainless steel exotic material.It is to be convenient to quick separated mycelia and medium that there is the protruding edge of shovel on each limit of front end of " U " type structure, and the limit of " U " type structure can also prevent that bacterial classification is unrestrained in switching process.
Beneficial effect
The invention provides a kind of novel inoculation shovel.Of the present invention having the following advantages: 1) simple to operate, 2) inoculation efficiency is high, 3) pollution rate is low, effective.The present invention is designed to parent edible fungus kind and turns and expand breeding reliable technical scheme is provided.
accompanying drawing explanation
A kind of end view that turns the novel inoculation shovel that expands breeding for parent edible fungus kind of Fig. 1.In figure: 1. handle 2. bodies of rod 3. shovel bodies.
embodiment
Below by embodiment, the present invention is described in further detail.In following embodiment, method therefor is conventional method if no special instructions.
Implementation method:
A kind of Novel edible starter kind inoculation shovel hoe, consists of handle, the body of rod, shovel body three parts.It can be made by manual or special producer.While making by hand, the stainless steel strip of an optional 30-35cm, first one section is located to carry out doubling about apart from its top 5 cm, as handle, the long part in its other end 1cm left and right is beaten into gradually thin slice trapezoidal shape, then both sides featheredge is vertically turned up into " U " type, make wide 4mm left and right, " U " bottom, the highest 2mm left and right, back gauge bottom, length of side 4mm left and right, with file processing, make " U " type become bladed in each limit foremost again.During inoculation, first inoculation shovel is carried out to flame sterilization, by " U " shovel hoe mouthful upwards by the 2mm left and right degree of depth on the medium of laterally drawing section forward horizontal sliding just can easily also once shovel out the square bacterial classification piece of a slice adhesion, move on sterilized fresh culture.

Claims (3)

1. a Novel edible fungi inoculation shovels, and this inoculation shovel consists of handle, the body of rod, shovel body three parts.
2. a kind of Novel edible fungi inoculation shovel according to claim 1, is characterized in that, described shovel body structure is " U " type, and front end has the edge of shovel of each limit projection.
3. a kind of Novel edible fungi inoculation shovel according to claim 1, is characterized in that, the material using is stainless steel exotic material.
CN201310512706.1A 2013-10-28 2013-10-28 Novel inoculating shovel for edible mushroom parent strain expansion breeding Pending CN103535190A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310512706.1A CN103535190A (en) 2013-10-28 2013-10-28 Novel inoculating shovel for edible mushroom parent strain expansion breeding

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310512706.1A CN103535190A (en) 2013-10-28 2013-10-28 Novel inoculating shovel for edible mushroom parent strain expansion breeding

Publications (1)

Publication Number Publication Date
CN103535190A true CN103535190A (en) 2014-01-29

Family

ID=49959360

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310512706.1A Pending CN103535190A (en) 2013-10-28 2013-10-28 Novel inoculating shovel for edible mushroom parent strain expansion breeding

Country Status (1)

Country Link
CN (1) CN103535190A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104148903A (en) * 2014-08-16 2014-11-19 宁波市鄞州云帆工程咨询有限公司 Method for manufacturing special bark chisel for inarching
CN116158309A (en) * 2022-11-09 2023-05-26 安徽槐耳生物工程技术中心(有限合伙) Sophora ear inoculation planter

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6194199B1 (en) * 1998-09-17 2001-02-27 Michael Hoffman Cell scraper device having blade on handles with pivotal joints
CN201204833Y (en) * 2007-12-31 2009-03-11 郭宏旗 Inoculation knife
CN201581077U (en) * 2009-11-30 2010-09-15 安徽省皖北药业股份有限公司 Improved microorganism inclined plane inoculating shovel
CN202754993U (en) * 2012-09-03 2013-02-27 齐齐哈尔大学 Inoculating shovel
CN103782800A (en) * 2013-10-28 2014-05-14 安徽科技学院 Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6194199B1 (en) * 1998-09-17 2001-02-27 Michael Hoffman Cell scraper device having blade on handles with pivotal joints
CN201204833Y (en) * 2007-12-31 2009-03-11 郭宏旗 Inoculation knife
CN201581077U (en) * 2009-11-30 2010-09-15 安徽省皖北药业股份有限公司 Improved microorganism inclined plane inoculating shovel
CN202754993U (en) * 2012-09-03 2013-02-27 齐齐哈尔大学 Inoculating shovel
CN103782800A (en) * 2013-10-28 2014-05-14 安徽科技学院 Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104148903A (en) * 2014-08-16 2014-11-19 宁波市鄞州云帆工程咨询有限公司 Method for manufacturing special bark chisel for inarching
CN116158309A (en) * 2022-11-09 2023-05-26 安徽槐耳生物工程技术中心(有限合伙) Sophora ear inoculation planter
CN116158309B (en) * 2022-11-09 2023-06-23 安徽槐耳生物工程技术中心(有限合伙) Sophora ear inoculation planter

Similar Documents

Publication Publication Date Title
CN102037849B (en) Tremella fuciformis strain culture method
CN104855141B (en) Edible mushroom liquid strain preparation method based on ultrasonic crushing
CN103535190A (en) Novel inoculating shovel for edible mushroom parent strain expansion breeding
CN103782800A (en) Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction
CN104521567B (en) Method for isolated culture of artificial strains of Helvella leucopus Pers
CN202197645U (en) Multifunctional inoculation scoop for plant tissue culture
CN203661669U (en) Novel vaccination shovel for edible fungus parent species trans-expansion breeding
CN103210845B (en) Method for obtaining aseptic seedling by using pinus massoniana seed
CN203633198U (en) Novel inoculating shovel for expanding propagation of mother strain of edible mushroom
CN201690811U (en) Growth and culture guide pipe for deep-root plants
CN205093217U (en) Mushroom fungus stick inoculation machine
CN201204833Y (en) Inoculation knife
CN206283898U (en) Multifunctional inoculation knife
CN205124610U (en) Flue -cured tobacco root exudates extraction element
CN102511310B (en) Cutting-opening tile-covering type bud inducement method for bag cultivation of grifola frondosa
CN205382161U (en) Modified fungi inclined plane inoculation shovel
CN105409586A (en) Novel multifunctional inoculating hook for edible fungi
CN203748384U (en) Tweezers knife specially used for tissue culture
CN204482425U (en) A kind of edible fungi inoculation hook
CN204634467U (en) A kind of edible mushroom organizes separator
CN106489524A (en) A kind of method of Phellinus igniarius (L. ex Fr.) Quel. rejuvenation of spawn
CN207252396U (en) A kind of multifunctional edible mushroom vaccinating lancet
CN209643481U (en) A kind of novel edible fungi inoculation tool
CN205196399U (en) Novel multi -functional domestic fungus inoculation hook
CN105624049A (en) Method for separating Sclerotinia homoeocarpa

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
C05 Deemed withdrawal (patent law before 1993)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140129