CN103782800A - Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction - Google Patents

Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction Download PDF

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Publication number
CN103782800A
CN103782800A CN201310512037.8A CN201310512037A CN103782800A CN 103782800 A CN103782800 A CN 103782800A CN 201310512037 A CN201310512037 A CN 201310512037A CN 103782800 A CN103782800 A CN 103782800A
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CN
China
Prior art keywords
shovel
hoe
edible fungus
inoculation
novel
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Pending
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CN201310512037.8A
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Chinese (zh)
Inventor
陆晓民
李慧敏
高青海
吴秀
李坤
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Anhui University of Science and Technology
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Anhui University of Science and Technology
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Application filed by Anhui University of Science and Technology filed Critical Anhui University of Science and Technology
Priority to CN201310512037.8A priority Critical patent/CN103782800A/en
Publication of CN103782800A publication Critical patent/CN103782800A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a novel inoculating shovel hoe used for edible fungus mother strain transplanting reproduction and belongs to the technical field of edible fungus inoculating. The novel inoculating shovel hoe comprises a handle, a rod and a shovel hoe body. The novel inoculating shovel hoe has the advantages of being simple to operate, quick and efficient, low in inoculating pollution rate, good in inoculating effect and the like, and is suitable for edible fungus mother strain transplanting reproduction or microorganism-related teaching and scientific research.

Description

A kind of novel inoculation shovel hoe that expands breeding that turns for parent edible fungus kind
Technical field
The present invention relates to a kind of inoculation shovel hoe that expands breeding that turns for parent edible fungus kind, belong to technical field of edible fungi inoculation.
Background technology
Edible fungus species, be edible mushroom good " seed ", for breeding, cultivate the pure mycelium fragment of edible mushroom of fruit body of edible fungi, it is just as the seed of crops, it is the lifeline of Edible Fungi, its quality can directly have influence on output and the quality of edible mushroom, and obtaining good bacterial classification is to obtain highly efficient and productive key point.Therefore the production of hybrid seeds of edible mushroom is an extremely important technical work in producing.
Edible fungus species is many by artificial culture, and its bacterial classification is pressed the difference in cultivation stage and source, can denominator kind, three kinds of original seed and cultivated speciess.Cultivate or organize separation cultivation, substrate mycelium to separate and cultivate the first generation mycelium obtaining from the spore separation of edible mushroom, be called female plant (being also test tube kind, one-level kind), more very thin because of it, a little less than decomposing the ability of nutriment, normal adopt to be rich on the agar medium that nutrition is easy to again absorb cultivate, and female kind before switching is cultivated as original seed, how need turn and expand for several times at test tube or female agar medium of planting flat-plate special blake bottle, to improve breeding amount, purification and rejuvenation or for provenance preservation etc.
Female kind is the mother of bacterial classification productions, and it expands numerous is a technical stronger job, require provenance single pure, medium sterilization is thorough, equipment disinfection is comprehensive, and operates in relatively aseptic environment, has absolutely not allowed miscellaneous bacteria infection.Currently turn for parent edible fungus kind the main inoculating tool that expands breeding and have inoculation hoe (inoculation rake) and inoculation to shovel (vaccinating lancet) two kinds of instruments, the inoculation hoe (inoculation rake) of current use is seemingly hoe (rake) the same instrument of a kind of front end with crotch, and inoculation shovel (vaccinating lancet) is the cutlery of a kind of front end with single-blade flat mouth.Turn in expansion breeding and need the two to be used in conjunction with in parent edible fungus kind, first long handle hoe (drag head) is goed deep in test tube or blake bottle first as bought bean curd medium to be carried out to horizontal cutting-up becoming some sections by it with inoculation hoe (inoculation rake), then carry out after 2 longitudinal cutting-ups on the both sides of laterally drawing section medium with inoculation shovel (vaccinating lancet), carry out again grafter from bottom, square body medium with mycelia is taken out, move on sterilized fresh culture.This method inoculation hoe, inoculation shovel all need through flame sterilization, and inoculation shovel (vaccinating lancet) enters former female kind and in pipe, need 3 cutting-ups and the easy landing of bacterium piece in the time taking out, and make the whole inoculation length that expends time in, and inoculation efficiency is low, has increased the infection chance of miscellaneous bacteria.How to utilize advanced inoculating tool, make inoculation operation faster, it is short that tube (bottle) opening time is tried one's best, and to reduce pollution rate, is the problem that is worth thinking.
Parent edible fungus kind of the present invention turns the inoculation shovel hoe that expands breeding, it integrates inoculation hoe (inoculation rake) and inoculation shovel (vaccinating lancet) two kinds of instruments, flame sterilization once, the medium that utilizes its center point effects on surface to be covered with silk downwards in " U " shovel hoe mouth carries out after crosscut, again by " U " shovel hoe mouth upwards by certain depth forward horizontal sliding just can be easily and adhesion ground once shovel out the square bacterial classification of a slice, and bacterium piece is difficult for landing in the time taking out, can save time, improve work efficiency, reduce and pollute.
Summary of the invention
The object of this invention is to provide a kind of design that turns the novel inoculation shovel of expansion breeding hoe for parent edible fungus kind, so that promote its extensive use in parent edible fungus kind is produced or tested.
The present invention is achieved in that
1. one kind turns and expands the novel inoculation shovel of breeding hoe for parent edible fungus kind, is made up of handle, the body of rod, shovel hoe body three parts.2. the shovel hoe body structure described in is " U " type, and front end has the shovel hoe sword of each limit projection.The material using is stainless steel exotic material.It is to be convenient to separate fast mycelia and medium that there is the protruding edge of shovel on the each limit of front end of " U " type structure, and the limit of " U " type structure can also prevent that bacterial classification is unrestrained in switching process.
Beneficial effect
The invention provides one turns and expands the novel inoculation shovel of breeding hoe for parent edible fungus kind.Of the present invention having the following advantages: 1) simple to operate, 2) inoculation efficiency is high, and 3) pollution rate is low, effective.The present invention is designed to parent edible fungus kind and turns and expand breeding reliable technical scheme is provided.
accompanying drawing explanation
A kind of end view that turns the novel inoculation shovel hoe that expands breeding for parent edible fungus kind of Fig. 1.In figure: 1. handle 2. body of rod 3. shovel hoe bodies.
Embodiment
Below by embodiment, the present invention is described in further detail.In following embodiment, method therefor is conventional method if no special instructions.
Implementation method:
A Novel edible starter kind inoculation shovel hoe, is made up of handle, the body of rod, shovel hoe body three parts.It can be made by manual or special producer.While making by hand, the stainless steel strip of an optional 30-35cm, first one section is being carried out to doubling apart from its 5 cm left and right places, top, as handle, long its other end 1cm left and right part is beaten into front end concordant, the selenodont gradually chip shape of sidepiece, then both sides featheredge is vertically turned up into " U " type, make wide 4mm left and right, " U " bottom, the highest 4mm left and right, back gauge bottom, again with file processing, make " U " type become bladed in each limit foremost, and " U " type becomes needle-like in 2mm left and right in top foremost, make front end have the shovel hoe sword of each limit projection, the flanging of " U " type lower vertical bottom is long for about 6mm.When inoculation, first inoculation shovel hoe is carried out to flame sterilization, the medium that utilizes its center point effects on surface to be covered with silk downwards in " U " shovel hoe mouth carries out after crosscut, again by " U " shovel hoe mouth upwards by the 2mm left and right degree of depth forward horizontal sliding just can be easily and adhesion ground once shovel out the square bacterial classification piece of a slice, move on sterilized fresh culture.

Claims (3)

1. one kind turns the novel inoculation shovel hoe that expands breeding for parent edible fungus kind, it is characterized in that, this inoculation shovel is made up of handle, the body of rod, shovel hoe body three parts.
2. a kind of Novel edible fungi inoculation shovel hoe according to claim 1, is characterized in that, described shovel hoe body structure is " U " type, and front end has the shovel hoe sword of each limit projection.
3. according to a kind of Novel edible fungi inoculation shovel hoe claimed in claim 1, it is characterized in that, the material using is stainless steel exotic material.
CN201310512037.8A 2013-10-28 2013-10-28 Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction Pending CN103782800A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310512037.8A CN103782800A (en) 2013-10-28 2013-10-28 Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction

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Application Number Priority Date Filing Date Title
CN201310512037.8A CN103782800A (en) 2013-10-28 2013-10-28 Novel inoculating shovel hoe used for edible fungus strain transplanting reproduction

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CN103782800A true CN103782800A (en) 2014-05-14

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103535190A (en) * 2013-10-28 2014-01-29 安徽科技学院 Novel inoculating shovel for edible mushroom parent strain expansion breeding
CN104148903A (en) * 2014-08-16 2014-11-19 宁波市鄞州云帆工程咨询有限公司 Method for manufacturing special bark chisel for inarching

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201204833Y (en) * 2007-12-31 2009-03-11 郭宏旗 Inoculation knife
CN201581077U (en) * 2009-11-30 2010-09-15 安徽省皖北药业股份有限公司 Improved microorganism inclined plane inoculating shovel
CN202197645U (en) * 2011-09-06 2012-04-25 广西亚热带作物研究所试验站 Multifunctional inoculation scoop for plant tissue culture
CN202465691U (en) * 2012-03-20 2012-10-03 成都康华生物制品有限公司 Spoon for collecting bacteria
CN202925020U (en) * 2012-11-08 2013-05-08 浙江出入境检验检疫局检验检疫技术中心 Bacterial block inoculator

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201204833Y (en) * 2007-12-31 2009-03-11 郭宏旗 Inoculation knife
CN201581077U (en) * 2009-11-30 2010-09-15 安徽省皖北药业股份有限公司 Improved microorganism inclined plane inoculating shovel
CN202197645U (en) * 2011-09-06 2012-04-25 广西亚热带作物研究所试验站 Multifunctional inoculation scoop for plant tissue culture
CN202465691U (en) * 2012-03-20 2012-10-03 成都康华生物制品有限公司 Spoon for collecting bacteria
CN202925020U (en) * 2012-11-08 2013-05-08 浙江出入境检验检疫局检验检疫技术中心 Bacterial block inoculator

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
侯保荣: "多刃接种刀的制作与应用", 《新疆农业科学》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103535190A (en) * 2013-10-28 2014-01-29 安徽科技学院 Novel inoculating shovel for edible mushroom parent strain expansion breeding
CN104148903A (en) * 2014-08-16 2014-11-19 宁波市鄞州云帆工程咨询有限公司 Method for manufacturing special bark chisel for inarching

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Application publication date: 20140514