CN103509844B - Low-acyl gellan gum extracting method - Google Patents
Low-acyl gellan gum extracting method Download PDFInfo
- Publication number
- CN103509844B CN103509844B CN201310347818.6A CN201310347818A CN103509844B CN 103509844 B CN103509844 B CN 103509844B CN 201310347818 A CN201310347818 A CN 201310347818A CN 103509844 B CN103509844 B CN 103509844B
- Authority
- CN
- China
- Prior art keywords
- liquid
- gelling gum
- gum
- low
- gellan gum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a low-acyl gellan gum extracting method. The extracting method comprises the following steps: fermenting, separating and removing mycoprotein, removing acyl groups, alcohol flocculating, membrane filtering, salt precipitating, and drying so as to extract the low-acyl gellan gum product. The invention also provides a composite bacterium liquid for producing high-acyl gellan gum. The extracting method provided by the invention is capable of improving the yield of low-acyl gellan gum, saves raw materials, can effectively recycle and utilize the waste, and is suitable for the industrial mass production.
Description
Technical field
The present invention relates to microbial technique production field, particularly relate to a kind of extracting method of low-acyl gellan gum.
Background technology
Gelling gum is the another novel microorganism exocellular polysaccharide after xanthan gum, there is excellent gellifying property, be be followed successively by D-Glucose by four glycan molecules, polymer that D-Glucose aldehydic acid, D-Glucose, L-rhamnosyl are formed by connecting by glycosidic link, wherein first glucose molecule connects with β-Isosorbide-5-Nitrae glycosidic link.Because gelling gum has superior gellifying property, it is widely used at field of food, as milk preparation, fruit squash, fruit spreads, pudding jelly and bread filler etc., be also applied in non food area, as the slow releasing, microbiological culture media etc. of toothpaste, medicine.
Gelling gum is applied in milk preparation, gelling gum is heated to 70 DEG C ~ 75 DEG C can direct hydration in milk, in acidophilous goods, add this kind of water-sol serve as colloid protective agent, the protein flocculation in milk preparation and the effect of mouthfeel can be eliminated; For in candy, superior structure and quality can be provided to product, and shorten the time of starch jelly colloid formation; Gelling gum joins the consumption that can reduce saturated fatty acid in biscuits, and improves the level of biscuit, makes biscuit have good sedimentation; Gelling gum also alternative pectin prepares jam and jelly, also can be used in cake and fruit pies filler; In the course of processing of meat product and greengrocery, adding gelling gum, can to make up the taste of product not enough, makes it have salubrious taste feature.
Usually gelling gum product is divided into two kinds according to the content of ethanoyl in gelling gum polysaccharide molecule: one is low-acyl gellan gum, by making deacylated tRNA base to high acyl gellan gum or the process of part deacylated tRNA base obtains, after aquation, under cation sites, easily form fragility gel; Another kind is high acyl gellan gum, and namely natural gelling gum, easily forms viscoelastic gel after aquation.Low-acyl gellan gum is not only stablized; and there is good gellifying property; compared with conventional gel agent (as carrageenin agar etc.); only need the consumption of 0.25%; the gel-strength that 1.5% agar and 1% carrageenin can reach can be reached; therefore low-acyl gellan gum has extensive use in fields such as food medical scientific research, has good market outlook.Prior art CN201210088884 discloses a kind of zymotechnique of gelling gum, and the method exists that gelling gum productive rate is not high, step is complicated and needs to add the problems such as pH adjusting agent.How to develop the technical problem that a kind of real prior art of the green extraction process that output is high and industrial energy consumption is low of gelling gum is anxious to be resolved.
Summary of the invention
In order to overcome the deficiencies in the prior art, improve the output of low-acyl gellan gum, conservation and waste reclaimation, the invention provides a kind of extracting method of low-acyl gellan gum, comprises following preparation process:
The first step, by mixed bacteria liquid, (Sphingomonas paucimobilis liquid and bacillus pumilus liquid weight ratio are that the concentration of thalline in 10: 1, two kinds of bacterium liquid is 1 × 10
8individual/mL) cultivate according in the inoculum size access seeding tank of 10% (volume ratio), it is 30 DEG C in temperature, under the condition of pH value 7.2, cultivate and obtain liquid A in 24 hours, then by liquid A: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 30 DEG C, pH value 7.2, incubation time 72 hours, obtains fermented liquid; Wherein, the component of seed tank culture base is: sucrose 25g, peptone 5g, ammonium sulfate 4.0g, yeast extract paste 3.0g, citric acid 3.5g, calcium carbonate 2.0g, distilled water 1000ml; The component of fermentation tank culture medium is: sucrose 40g, peptone 8g, potassium primary phosphate 3g, citric acid 2.5g, calcium carbonate 2.0g, magnesium sulfate 1g, distilled water 1000ml;
Second step, in the fermented liquid that the first step obtains, add the mixture of diatomite and aerosil, interpolation limit, limit is stirred simultaneously, and stir 10min, stirring velocity is 100r/min, then uses Plate Filtration, collects the filtrate after filtering and thalline respectively; The addition of wherein said mixture is 0.5% of fermented liquid weight, and the mass ratio of described diatomite and aerosil is 3: 1; The specific surface area of described aerosil is more than 200m2/g;
3rd step, adds liquid caustic soda and regulates pH to be 10, be warming up to 90 DEG C ~ 95 DEG C, maintain 30 ~ 40 minutes, carry out the process of deacylated tRNA base, be then down to room temperature in the filtrate that second step obtains; Add 70% ~ 90% ethanol of 2 ~ 3 times of volumes, mix, flocculation gelling gum; Then through membrane filtration removing waste liquid, the gelling gum that flocculates is obtained; And after the ethanol in low-temperature rotary evaporation Recycling of waste liquid, obtain liquid B; Above-mentioned flocculation gelling gum again disperseed and is dissolved in water, obtains gelling gum liquid, then adding the saturated nacl aqueous solution of gelling gum liquid volume 6% ~ 7%, Precipitation gelling gum;
4th step, the gelling gum of the 3rd step being separated out, through screw press, is removed unnecessary moisture, is obtained wet product gelling gum; Then by dry for wet product gelling gum, pulverize and obtain low-acyl gellan gum;
5th step, the liquid B that the thalline obtain second step and the 3rd step obtain is mixed to get mixing solutions, and then in mixing solutions, add Semen Maydis powder, sorghum flour, bean dregs and wheat bran, interpolation limit, limit is stirred to pasty state; Finally pass into steam and be warming up to 110 DEG C, distill 15 minutes; Then by after distillment oven dry, pulverizing, add zinc sulfate, dimension dirt element E and chlorogenic acid, mix, acquisition pig feed; Wherein, Semen Maydis powder, sorghum flour, bean dregs and wheat bran account for 6%, 5%, 2% and 1% of mixing solutions quality respectively, zinc sulfate, vitamin-E and chlorogenic acid account for respectively mixing solutions quality ten thousand/.
The invention also discloses a kind of composite bacteria liquid producing low-acyl gellan gum, it is to mix at 10: 1 by Sphingomonas paucimobilis liquid and bacillus pumilus liquid according to weight ratio, and in two kinds of bacterium liquid, the concentration of thalline is all about 1 × 10
8individual/mL.
The Sphingomonas paucimobilis that the present invention uses and the bacterial strain that bacillus pumilus is commonly used for this area, (ATCC 31461, see J Ind Microbiol Biotechnol.2002Oct for preferred Sphingomonas paucimobilis Sphingomonas paucimobilis; 29 (4): 170-6.) and bacillus pumilus (Bacillus pumilus) (ATCC27142, see Journal of FoodProtection1995, Volume58, Number4).
The beneficial effect that the present invention obtains is mainly as follows:
By test of many times and research, in Sphingomonas paucimobilis fermentation, with the addition of bacillus pumilus pioneeringly, make bacillus pumilus can effectively degrade Sphingomonas paucimobilis produce all kinds of acidic substance, and under making fermented liquid maintain weak basic condition all the time, keep Sphingomonas paucimobilis ferment effect to maintain higher state;
The Optimal pH that Sphingomonas paucimobilis fermentation produces gelling gum is about 7.2, but multiple acidic substance can be produced during the fermentation, comprise hydroxybutyric acid etc., yeasting is developed toward acidic conditions, do not utilize the fermentation of Sphingomonas paucimobilis, the output of gelling gum is reduced, and bacillus pumilus is by the collaborative symbiosis with Sphingomonas paucimobilis, facilitates the generation of gelling gum; And reduce the content of all kinds of acidic substance in gelling gum, improve the purity of gelling gum.
Without the need to additionally adding pH adjusting agent in fermenting process, decreasing the waste of raw material, having saved cost;
Decrease all kinds of chemical substances that in prior art, other extracting method use in leaching process, avoid and chemical substance is brought in gelling gum;
In the process extracting gelling gum, tropina, macromolecular polysaccharide etc. are reclaimed, avoids the waste liquid pollution on the environment of this type of material; Meanwhile, obtain nutritious animal feeding-stuff containing somatic protein, turn waste into wealth, increase economic benefit; Achieve the environmental protection of gelling gum leaching process simultaneously, almost arrange outward without waste water, feed liquid component makes full use of.
Embodiment
Below employing specific embodiment is further explained the present invention, but should not regards the restriction to initiative spirit of the present invention as.
Embodiment 1
An extracting method for low-acyl gellan gum, comprises following preparation process:
The first step, by mixed bacteria liquid, (Sphingomonas paucimobilis liquid (ATCC31461) and bacillus pumilus liquid (ATCC27142) weight ratio are that the concentration of thalline in 10: 1, two kinds of bacterium liquid is 1 × 10
8individual/mL) cultivate according in the inoculum size access seeding tank of 10% (volume ratio), it is 30 DEG C in temperature, under the condition of pH value 7.2, cultivate and obtain liquid A in 24 hours, then by liquid A: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 30 DEG C, pH value 7.2, incubation time 72 hours, obtains fermented liquid; Wherein, the component of seed tank culture base is: sucrose 25g, peptone 5g, ammonium sulfate 4.0g, yeast extract paste 3.0g, citric acid 3.5g, calcium carbonate 2.0g, distilled water 1000ml; The component of fermentation tank culture medium is: sucrose 40g, peptone 8g, potassium primary phosphate 3g, citric acid 2.5g, calcium carbonate 2.0g, magnesium sulfate 1g, distilled water 1000ml;
Second step, in the fermented liquid that the first step obtains, add the mixture of diatomite and aerosil, interpolation limit, limit is stirred simultaneously, and stir 10min, stirring velocity is 100r/min, then uses Plate Filtration, collects the filtrate after filtering and thalline respectively; The addition of wherein said mixture is 0.5% of fermented liquid weight, and the mass ratio of described diatomite and aerosil is 3: 1; The specific surface area of described aerosil is 200m
2/ more than g;
3rd step, adds liquid caustic soda (having another name called neat liquid caustic soda) and regulates pH to be 10, be warming up to 90 DEG C, maintain 35 minutes, carry out the process of deacylated tRNA base, be then down to room temperature in the filtrate that second step obtains; Add 80% ethanol of 2 times of volumes, mix, flocculation gelling gum; Then through membrane filtration removing waste liquid, the gelling gum that flocculates is obtained; And after the ethanol in low-temperature rotary evaporation Recycling of waste liquid, obtain liquid B; Above-mentioned flocculation gelling gum again disperseed and is dissolved in water, obtains gelling gum liquid, then adding the saturated nacl aqueous solution of gelling gum liquid volume 7%, Precipitation gelling gum;
4th step, the gelling gum of the 3rd step being separated out, through screw press, is removed unnecessary moisture, is obtained wet product gelling gum; Then by dry for wet product gelling gum, pulverize and obtain low-acyl gellan gum;
5th step, the liquid B that the thalline obtain second step and the 3rd step obtain is mixed to get mixing solutions, and then in mixing solutions, add Semen Maydis powder, sorghum flour, bean dregs and wheat bran, interpolation limit, limit is stirred to pasty state; Finally pass into steam and be warming up to 110 DEG C, distill 15 minutes; Then by after distillment oven dry, pulverizing, add zinc sulfate, vitamin-E and chlorogenic acid, mix, obtain pig feed; Wherein, Semen Maydis powder, sorghum flour, bean dregs and wheat bran account for 6%, 5%, 2% and 1% of mixing solutions quality respectively, zinc sulfate, vitamin-E and chlorogenic acid account for respectively mixing solutions quality ten thousand/.
Embodiment 2
The performance perameter of low-acyl gellan gum prepared by embodiment 1:
Control group: only adopt Sphingomonas paucimobilis fermentative production gelling gum, other steps are with embodiment 1; Experimental group is gelling gum prepared by embodiment 1.The molecular weight determination content of acyl group (in the determination of acid-basetitration gelling gum): control group low-acyl gellan gum molecular-weight average all exists: 760,000 dalton, and the molecular-weight average of the gelling gum of experimental group all exists: 740,000 dalton.
The mensuration of gel-strength: carry out the mensuration measuring transmittance with TA.TX21 property tester;
The mensuration of transmittance: take 0.5 sample, adding distil water 100ml, beaker is placed in 80 C water bath, the calcium chloride solution 2ml of 2.7% is added after sample dissolution, supplement evaporated water to original volume, while hot by sol solution impouring cuvette, the thermostat container putting into 20 degrees Celsius is immediately placed 15 minutes, measure transmittance with spectrophotometer at 497nm place, contrast with distilled water.
Conclusion: experimental group and control group do not have significant difference in colloid purity and transmittance and gel-strength etc., but colloid output is far longer than control group.
The test of embodiment 3 pig feed of the present invention:
After testing, pig feed protein content 38.2% prepared by embodiment 1, polysaccharose substance content 25.2%, inorganic mineral content 2.7%, all the other are starch, Mierocrystalline cellulose and a small amount of trace element etc.
Choose a month large weanling pig 200, be divided into two groups, often organize 100, wherein the diet prepared of experimental group the present invention, every 50kg is 210 yuan, and control group, with honest feed (SSB-25 model), is 300 yuan of calculating according to every 50kg.Raise after 6 weeks and detect indices see table 1.
Table 1
| Index (every piglet) | Control group | Of the present invention group |
| Weanling pig body weight (kg) | 4.87 | 4.91 |
| The body weight (kg) increased for 6 weeks | 13.87 | 14.92 |
| Consume feed (kg) | 16.2 | 17.4 |
| Feed for nursing cost (unit) | 97.2 | 73.1 |
Conclusion: the pig feed cost that the present invention utilizes waste material to prepare is starkly lower than market common feedstuffs, and the increase of body weight is also greater than control group, possesses good using value, and turns waste into wealth, kill two birds with one stone.
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (1)
1. an extracting method for low-acyl gellan gum, comprises following preparation process:
The first step, mixed bacteria liquid is cultivated according in the inoculum size access seeding tank of 10%, it is 30 DEG C in temperature, under the condition of pH value 7.2, cultivate and obtain liquid A, then by liquid A in 24 hours: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 30 DEG C, pH value 7.2, incubation time 72 hours, obtains fermented liquid; Wherein, the component of seed tank culture base is: sucrose 25g, peptone 5g, ammonium sulfate 4.0g, yeast extract paste 3.0g, citric acid 3.5g, calcium carbonate 2.0g, distilled water 1000mL; The component of fermentation tank culture medium is: sucrose 40g, peptone 8g, potassium primary phosphate 3g, citric acid 2.5g, calcium carbonate 2.0g, magnesium sulfate 1g, distilled water 1000mL; Described mixed bacteria liquid is to mix at 10: 1 by Sphingomonas paucimobilis liquid and bacillus pumilus liquid according to weight ratio, and in described Sphingomonas paucimobilis liquid or described bacillus pumilus liquid, the concentration of thalline is 1 × 10
8individual/mL;
Second step, the fermented liquid toward the first step acquisition adds the mixture of diatomite and aerosil, and interpolation limit, limit is stirred, and stir 10min, stirring velocity is 100r/min, then uses Plate Filtration, collects the filtrate after filtering and thalline respectively; The addition of wherein said mixture is 0.5% of fermented liquid weight, and the mass ratio of described diatomite and aerosil is 3: 1;
3rd step, adds liquid caustic soda and regulates pH to be 10, be warming up to 90 DEG C ~ 95 DEG C, maintain 30 ~ 40 minutes, carry out the process of deacylated tRNA base, be down to room temperature in the filtrate that second step obtains; Then add 70% ~ 90% ethanol of 2 ~ 3 times of volumes, mix, flocculation gelling gum; Then through membrane filtration removing waste liquid, the gelling gum that flocculates is obtained; And after the ethanol in low-temperature rotary evaporation Recycling of waste liquid, obtain liquid B; Above-mentioned flocculation gelling gum again disperseed and is dissolved in water, obtains gelling gum liquid, then adding the saturated nacl aqueous solution accounting for gelling gum liquid volume 6% ~ 7%, Precipitation gelling gum;
4th step, the gelling gum of the 3rd step being separated out, through screw press, is removed unnecessary moisture, is obtained wet product gelling gum; Then by dry for wet product gelling gum, pulverize and obtain low-acyl gellan gum;
5th step, the liquid B that the thalline obtain second step and the 3rd step obtain is mixed to get mixing solutions, and then in mixing solutions, add Semen Maydis powder, sorghum flour, bean dregs and wheat bran, interpolation limit, limit is stirred to pasty state; Finally pass into steam and be warming up to 110 DEG C, distill 15 minutes; Then by after distillment oven dry, pulverizing, add zinc sulfate, vitamin-E and chlorogenic acid, mix, obtain pig feed; Wherein, Semen Maydis powder, sorghum flour, bean dregs and wheat bran account for 6%, 5%, 2% and 1% of mixing solutions quality respectively, zinc sulfate, vitamin-E and chlorogenic acid account for respectively mixing solutions quality ten thousand/;
Described Sphingomonas paucimobilis is Sphingomonas paucimobilis (Sphingomonas paucimobilis) ATCC 31461, and described bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC 27142.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310347818.6A CN103509844B (en) | 2013-08-09 | 2013-08-09 | Low-acyl gellan gum extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310347818.6A CN103509844B (en) | 2013-08-09 | 2013-08-09 | Low-acyl gellan gum extracting method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103509844A CN103509844A (en) | 2014-01-15 |
| CN103509844B true CN103509844B (en) | 2015-05-13 |
Family
ID=49893276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310347818.6A Active CN103509844B (en) | 2013-08-09 | 2013-08-09 | Low-acyl gellan gum extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103509844B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862354A (en) * | 2014-02-24 | 2015-08-26 | 杨凤英 | Production method of low-acyl gellan gum used for oil and gas exploitation |
| CN104193841B (en) * | 2014-08-07 | 2016-08-24 | 新疆阜丰生物科技有限公司 | A kind of low cost low acyl transparent type gellan gum extraction process |
| CN107287260B (en) * | 2016-04-11 | 2020-09-15 | 新疆梅花氨基酸有限责任公司 | Production method of low-cost high-quality xanthan gum |
| CN106350554A (en) * | 2016-08-30 | 2017-01-25 | 新疆阜丰生物科技有限公司 | Extraction method of non-transparent type low-acyl gellan gum |
| CN111961697B (en) * | 2020-07-27 | 2023-05-30 | 鄂尔多斯市中轩生化股份有限公司 | Production process of low-acyl xanthan gum |
| CN116023520B (en) * | 2023-01-17 | 2023-08-22 | 河北沣川生物科技有限公司 | Preparation method of low-acyl sanzan gum |
| CN116135886A (en) * | 2023-04-18 | 2023-05-19 | 广州市乾相生物科技有限公司 | Extraction method of transparent low-acyl gellan gum |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182089A (en) * | 2007-11-15 | 2008-05-21 | 山东阜丰生物科技开发有限公司 | Xanthan gum waste water treatment process |
| CN101591399A (en) * | 2009-07-09 | 2009-12-02 | 浙江中肯生物科技有限公司 | A kind of low-acyl gellan gum extracting method that is applicable to tissue culture medium (TCM) |
| CN101591400A (en) * | 2009-07-09 | 2009-12-02 | 浙江中肯生物科技有限公司 | A kind of method for post extraction of low-acyl clean-type gellan gum |
| CN101824095A (en) * | 2009-05-31 | 2010-09-08 | 上海众伟生化有限公司 | Transparent high acyl gellan gum and production method thereof |
| CN102199640A (en) * | 2011-04-15 | 2011-09-28 | 江南大学 | Energy-saving production method of gellan gum |
-
2013
- 2013-08-09 CN CN201310347818.6A patent/CN103509844B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182089A (en) * | 2007-11-15 | 2008-05-21 | 山东阜丰生物科技开发有限公司 | Xanthan gum waste water treatment process |
| CN101824095A (en) * | 2009-05-31 | 2010-09-08 | 上海众伟生化有限公司 | Transparent high acyl gellan gum and production method thereof |
| CN101591399A (en) * | 2009-07-09 | 2009-12-02 | 浙江中肯生物科技有限公司 | A kind of low-acyl gellan gum extracting method that is applicable to tissue culture medium (TCM) |
| CN101591400A (en) * | 2009-07-09 | 2009-12-02 | 浙江中肯生物科技有限公司 | A kind of method for post extraction of low-acyl clean-type gellan gum |
| CN102199640A (en) * | 2011-04-15 | 2011-09-28 | 江南大学 | Energy-saving production method of gellan gum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103509844A (en) | 2014-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103509844B (en) | Low-acyl gellan gum extracting method | |
| CN101665384B (en) | Se-enriched bio-organic fertilizer and producing method thereof | |
| CN101665778B (en) | Uranidin generation deficiency sphingolipid sphingomonas paucimobilis and application thereof in gellan gum production | |
| CN102894447B (en) | Ganoderma lucidum polysaccharide health-care drink and production method thereof | |
| CN102669780A (en) | Technique for fermentation production of fruit vinegar beverage by utilizing orange peel residue | |
| CN102318790A (en) | A kind of bean vermicelli leavening and the method for producing the fermentation bean vermicelli thereof | |
| CN103436403B (en) | Highland barley za wine and preparation method thereof | |
| CN103409492B (en) | Method for extracting transparent low-acyl gellan gum | |
| CN101589151A (en) | Method for production of ethanol or lactic acid | |
| CN104543400A (en) | Preparation method of fermentation state zinc feed additive | |
| CN102783565B (en) | Preparation method of cordyceps feed additive | |
| CN1318582C (en) | Once aspergillus niger fermentation process of obtaining complex enzyme of several kinds of enzyme and with high enzyme activity | |
| CN103275875A (en) | Trichoderma koningii, and compound microbial agent composition and application thereof | |
| CN117229958A (en) | Xanthomonas campestris and application thereof in preparing low-viscosity xanthan gum | |
| CN104116000B (en) | A kind of preparation method of FOS feed addictive | |
| CN101560261B (en) | Cleaner production method of gellan gum | |
| CN107574075A (en) | A kind of waxy corn wine brewage technology | |
| CN107594416A (en) | A kind of processing method for the betel nut that ferments | |
| CN109699812A (en) | Solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method | |
| CN105586368B (en) | A kind of method of the processing method and fermentation production of citric acid of sorghum seed | |
| CN103392920A (en) | Fermentation method of soybean hulls | |
| CN102010857B (en) | Method for producing acid xylanase from whey powder | |
| CN103509845B (en) | High aryl gellan gum extracting method | |
| CN103436587B (en) | Purifying method of transparent type high-acyl gellan gum | |
| CN102943049B (en) | Process for producing cryytococcus neoformans culture through solid fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |