CN103509841A - Production technology for transforming cordyceps militaris waste residue and scrap into antioxidant peptide functional factor - Google Patents
Production technology for transforming cordyceps militaris waste residue and scrap into antioxidant peptide functional factor Download PDFInfo
- Publication number
- CN103509841A CN103509841A CN201210217121.2A CN201210217121A CN103509841A CN 103509841 A CN103509841 A CN 103509841A CN 201210217121 A CN201210217121 A CN 201210217121A CN 103509841 A CN103509841 A CN 103509841A
- Authority
- CN
- China
- Prior art keywords
- functional factor
- waste residue
- production technology
- cordyceps militaris
- technology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a production technology for transforming cordyceps militaris waste residue and scrap into an antioxidant peptide functional factor, and belongs to the technical field of biochemical engineering. In the production technology, waste residue and scrap generated during the production process of cordyceps militaris are used as raw materials, by virtue of an ultrasonic technology, a controllable enzymolysis technology for compounding various proteases and an ultrafiltration membrane and chromatographic separation technology, a small molecule peptide functional factor with biological activity is prepared through stepwise separation, and the small molecule peptide functional factor has ideal antioxidant and anti-aging functions. According to the production technology established by the invention, the extracted and separated small molecule peptide functional factor not only has remarkable biological activity, but also has the characteristics that the cost is low, the raw materials are easily available, the preparation process is simple, waste is changed into things of value, and the concept of environmental friendliness is satisfied. More importantly, the production technology can be used for realizing large scale production, and has the advantages of better stability, high safety index and the like. The production technology can be used in the fields of pharmacy, health care products and foods, cosmetics and the like. Meanwhile, the production technology can be used for realizing comprehensive utilization of the waste residue and scrap in the production process of the cordyceps militaris and improving the resource development availability and has certain practical value.
Description
Technical field:
The invention belongs to biochemical engineering technical field, relate to extraction separation, enzymolysis and the purifying process process of utilizing Cordyceps militaris waste residue waste material to transform antioxygenic micromolecule peptide functional factor.
Background technology:
Utilize the small-molecular peptides functional factor of the production technique acquisition of this invention foundation, to using the waste residue waste material that produces in Cordyceps militaris production process as raw material, with ultrasonic technology, the composite Controlled-enzymatic Hydrolysis technology of multiple protein enzyme, and integrated ultra-filtration membrane isolation technique step by step separation prepared and there is bioactive small-molecular peptides functional factor, through ultraviolet spectrometry degree meter detection level, reach 14%.Biological activity assay shows: its resistance of oxidation (gsh method), the Scavenging action to hydroxyl free radical 138.47% of Triptide, and this small-molecular peptides functional factor is 1453.33%, in the brown matter fat of plastosome growing amount index detects, concentration increase along with small-molecular peptides, the growing amount of brown matter fat reduces gradually, when the amount of small-molecular peptides functional factor is > 25mg, it is original 63.12% that inhibiting rate reaches, and has desirable anti-oxidant and antidotal biological function.Use technique of the present invention, extract separated small-molecular peptides functional factor and not only there is significant biological activity, also have that cost is low, raw material is easy to get, preparation process is simple, turns waste into wealth, meet the feature of environmental protection concept.The more important thing is can scale operation, and have the advantages such as good stability and safety index height.
Summary of the invention:
The object of the invention is to set up a kind of by the waste residue waste material producing in Cordyceps militaris production process, change into the production technique with biological activity small-molecular peptides functional factor.
Concrete technology technology implementation mode:
1, the pre-treatment of waste residue waste material
Bright Chinese caterpillar fungus waste residue waste material is put to 40 ℃ and dry to constant weight, with pulverizer, pulverize, cross 80 mesh sieves, get quantitative waste residue waste material dry powder, with solvent (deionized water), soak into dry powder and covered solid surface, put room-temperature water bath and soak 1h.
2, ultrasonic disruption
By above-mentioned solution ultrasonication 10～20s， interval 10～20s under 50～100W condition, 3～5 times repeatedly, use deionized water constant volume, ratio: 50/1000 (g/ml).
3, composite enzymolysis
Above-mentioned solution is heated to 45～60 ℃, and interval 30min after constant temperature, adds cellulase and papoid in 3% and 5% ratio respectively successively, after enzymolysis 3h, and 100 ℃ of enzyme 20min that go out, cooling.
4, ultrafiltration and chromatography
By above-mentioned solution with after 4 layers of filtered through gauze, ultra-filtration membrane ultrafiltration with molecular weight 100,000～50,000, use again the ultra-filtration membrane ultrafiltration of molecular weight 10,000～0.3 ten thousand, obtain 1 liter of greenish orange yellow liquid, then set up the Matrex Cellufine Sulfate affinity chromatography chromatography purification method of connecting with the multicolumn of Sepharose4FF gel-filtration.After crossing film degerming, bottling sealing is standby.
5. content and biological activity assay
According to above-mentioned technique, 100g raw material obtains the about 6mg/ml of small-molecular peptides functional factor (stoste).Antioxidant Indexes detected result (gsh method): in Isodose situation, the Scavenging action to hydroxyl free radical 138.47% of Triptide, and this small-molecular peptides functional factor is 1334.33%, in the brown matter fat of plastosome growing amount index detects, when the amount of small-molecular peptides functional factor is > 25mg, inhibiting rate reaches original 63.12%.
Practical significance of the present invention:
Small-molecular peptides functional factor is the oligopeptides with important biomolecule activity.Being the important chemical messenger of a class of linking up information between iuntercellular and organ, by modes such as internal secretion, neurosecretions, exercising delicate information transfer function, is the important conditioning agent of the vital processes such as body growth, growth, breeding, metabolism.By dipeptides to the oligopeptides of complicated linearity or ring type structure not etc., the effect of these small-molecular peptides functional factors makes body maintain its a series of high-density Controlling System to its molecular weight distribution just.It is worth noting that some protein itself there is no physiological action or effect is very low, and its hydrolysis fragment has very strong physiologically active.The present invention obtains that small-molecular peptides functional factor is safe, and enzymolysis process is easily controlled, and its physiological function is strong in addition, has the unique advantage that consumption is few.Its anti-oxidant and antidotal function, can be for fields such as pharmacy, healthcare products and food, makeup.The comprehensive utilization that has simultaneously solved waste residue waste material in Cordyceps militaris production process, has improved resources development and utilization degree, has certain practical value.
Claims (4)
1. utilize Cordyceps militaris waste residue waste material to transform the production technique of anti-oxidation peptide functional factor, it is characterized in that comprising the following steps:
(1) pre-treatment of waste residue waste material: bright Chinese caterpillar fungus waste residue waste material is put to 40 ℃ and dry to constant weight, with pulverizer, pulverize, cross 80 mesh sieves, get quantitative waste residue waste material dry powder, with solvent (deionized water), soak into dry powder and covered solid surface, put room-temperature water bath and soak 1h.
(2) ultrasonic disruption: by above-mentioned solution ultrasonication 10～20s， interval 10～20s under 50～100W condition, 3～5 times repeatedly, use deionized water constant volume, ratio: 50/1000 (g/ml).
(3) composite enzymolysis: above-mentioned solution is heated to 45～60 ℃, and interval 30min after constant temperature, adds cellulase and papoid in 3% and 5% ratio respectively successively, after enzymolysis 3h, 100 ℃ of enzyme 20min that go out, cooling.
(4) ultrafiltration and chromatography: by above-mentioned solution with after 4 layers of filtered through gauze, ultra-fine filter membrane filtration with molecular weight 100,000～50,000, use again the ultra-filtration membrane ultrafiltration of molecular weight 10,000～0.3 ten thousand, obtain 1 liter of greenish orange yellow liquid, then set up the Matrex Cellufine Sulfate affinity chromatography chromatography purification method of connecting with the multicolumn of Sepharose4FF gel-filtration.After crossing film degerming, bottling sealing is standby.
(5) content and biological activity assay: according to above-mentioned technique, 100g raw material obtains the about 6mg/ml of small-molecular peptides functional factor (stoste).Antioxidant Indexes detected result (gsh method): in Isodose situation, the Scavenging action to hydroxyl free radical 138.47% of Triptide, and this small-molecular peptides functional factor is 1334.33%, in the brown matter fat of plastosome growing amount index detects, when the amount of small-molecular peptides functional factor is > 25mg, inhibiting rate reaches original 63.12%.
2. method according to claim 1, is characterized in that described proteolytic enzyme is cellulase and papoid, and the ratio 3% and 5% of enzymolysis substrate.
3. method according to claim 1, the Matrex Cellufine Sulfate affinity chromatography chromatography purification method of connecting with the multicolumn of Sepharose4FF gel-filtration that it is characterized in that described foundation.
4. the described production process in (1)～(4) in method according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210217121.2A CN103509841A (en) | 2012-06-28 | 2012-06-28 | Production technology for transforming cordyceps militaris waste residue and scrap into antioxidant peptide functional factor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210217121.2A CN103509841A (en) | 2012-06-28 | 2012-06-28 | Production technology for transforming cordyceps militaris waste residue and scrap into antioxidant peptide functional factor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103509841A true CN103509841A (en) | 2014-01-15 |
Family
ID=49893273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210217121.2A Pending CN103509841A (en) | 2012-06-28 | 2012-06-28 | Production technology for transforming cordyceps militaris waste residue and scrap into antioxidant peptide functional factor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103509841A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112250731A (en) * | 2020-10-22 | 2021-01-22 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Green functional factor food preparation equipment and preparation method thereof |
-
2012
- 2012-06-28 CN CN201210217121.2A patent/CN103509841A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 刘艳芳等: "大孔树脂分离纯化发酵液中虫草素的研究", 《食品与生物技术学报》 * |
| 谢红旗等: "酶法提取蛹虫草中虫草素的研究", 《时珍国医国药》 * |
| 韩晨: "蛹虫草酶解液抗氧化活性研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
| 韩晨等: "响应曲面优化酶法制备蛹虫草抗氧化活性成分的研究", 《食品研究与开发》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112250731A (en) * | 2020-10-22 | 2021-01-22 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Green functional factor food preparation equipment and preparation method thereof |
| CN112250731B (en) * | 2020-10-22 | 2022-05-03 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Green functional factor food preparation equipment and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103757084B (en) | A kind of rice wine vinasse extract and its preparation method and application | |
| CN102600056B (en) | Essential oil added type skin care product | |
| CN110623860B (en) | Giant salamander active peptide and hyaluronic acid composition capable of effectively promoting fibroblast proliferation | |
| WO2022116807A1 (en) | Method for preparing ganoderma lucidum extract oil rich in ganoderma lucidum triterpene | |
| CN108157579A (en) | A kind of preparation method of the Cardamine violifolia selenium polypeptide of high organic selenium content | |
| CN104706567B (en) | The method and product of pearl foetal sac liquid are extracted in a kind of pearl foetal sac from mother pearl shellfish | |
| GB2590263A (en) | Peanut antioxidative peptide prepared from high pressure-assisted enzymatic hydrolysis and preparation method therefor | |
| CN104513307A (en) | Method for recovery of Kunitz and Bowman-Birk trypsin inhibitors from soybean whey | |
| CN105132491A (en) | Pachymaran extracting method | |
| CN109207544A (en) | A kind of preparation method of chlorella antioxidation polypeptide | |
| CN104988199A (en) | Preparation method of mucus protein peptide of andrias davidianus | |
| CN103333222A (en) | Pearl natural active peptide having whitening efficacy, and extraction method and application thereof | |
| CN102492049A (en) | Method of extracting polysaccharide, volatile oil and lignans from Schisandra chinensis | |
| CN102093748B (en) | Method for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes | |
| CN102630943B (en) | Spirulina gamma-linolenic acid extractive and preparation method thereof | |
| CN104402975A (en) | Anti-aging short peptide and preparation method thereof | |
| CN103509841A (en) | Production technology for transforming cordyceps militaris waste residue and scrap into antioxidant peptide functional factor | |
| CN102838684B (en) | Separating and purifying process of isochrysis galbana exopolysaccharide | |
| CN105557306B (en) | A kind of method using dwarf lilyturf slag fermenting and producing medicinal fungal substance | |
| CN104231105B (en) | The preparation method of a kind of sea anemone polysaccharide and antitumor application thereof | |
| CN114766681B (en) | Composition for relieving physical fatigue and preparation method thereof | |
| CN102399129B (en) | Method for extracting 1-octacosanol from bagasse | |
| CN109748951A (en) | A kind of Radix Angelicae Sinensis antioxidation polypeptide and the preparation method and application thereof | |
| CN1332019C (en) | Grifola umbellate zymophyte powder and grifola umbellate polysaccharide producing method and products therefrom | |
| CN105131059B (en) | A method of extracting geniposide from cape jasmine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140115 |