CN114766681B - Composition for relieving physical fatigue and preparation method thereof - Google Patents
Composition for relieving physical fatigue and preparation method thereof Download PDFInfo
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- CN114766681B CN114766681B CN202210200815.9A CN202210200815A CN114766681B CN 114766681 B CN114766681 B CN 114766681B CN 202210200815 A CN202210200815 A CN 202210200815A CN 114766681 B CN114766681 B CN 114766681B
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a composition for relieving physical fatigue and a preparation method thereof, wherein the composition comprises jellyfish polypeptide and American ginseng extract, the mass fraction of the jellyfish polypeptide is 80-90%, and the mass fraction of the American ginseng extract is 10-20%; the jellyfish polypeptide is an enzymolysis product of a desalted jellyfish umbrella part or a wrist part. The composition disclosed by the invention combines jellyfish polypeptide of ocean source and American ginseng extract of land source, and has good effect, easy absorption, no toxicity and no side effect. In the preparation method of the composition provided by the invention, the desalted jellyfish is directly hydrolyzed into jellyfish polypeptide, so that the utilization rate of jellyfish protein is high, the operation is simple and convenient, and no pollution is caused. The invention improves the value of jellyfish resources from the two aspects of the utilization rate of jellyfish raw materials and the high added value of products.
Description
Technical Field
The invention belongs to the technical field of marine organisms, and particularly relates to a composition for relieving physical fatigue and a preparation method thereof.
Background
With the increase in pace of life and increased working pressure, physical fatigue has become a very common public health problem. WHO surveys show that more than 35% of people worldwide are in fatigue. Long-time physical fatigue is easy to cause mental system symptoms such as inattention, sleep disorder, depression and the like, thereby influencing normal work, study and life. Long-term fatigue can also lead to immune disorders, hormonal disorders, organic diseases, etc.
Jellyfish belongs to coelenterates, more than 400 jellyfish are recorded in China, and the jellyfish accounts for about one third of the recorded types in the world. Jellyfish is rich in resources and wide in distribution, and is distributed from the coast of the south China island to the coast of the Shandong China Liaoning. In recent years, jellyfish outbreaks have become the most serious marine organism disasters in addition to red tides due to the influence of global climate change and other factors. Jellyfish contains abundant proteins, and the research on the proteins in jellyfish at the present stage mainly focuses on extracting collagen through acid, alkali, hot water or enzymatic hydrolysis, and hydrolyzing the collagen into collagen peptide. During the extraction of jellyfish collagen, a large amount of acid-soluble or alkali-soluble protein is discarded, resulting in low utilization of jellyfish protein. How to efficiently utilize the protein in jellyfish is the core for establishing jellyfish resource high-value utilization and jellyfish disaster prevention and reduction technology.
Although some related technologies using jellyfish peptides are disclosed in the prior art, specific biological objects in the disclosed materials are relatively single, and extraction technology of application components is complex and extraction rate is low.
Disclosure of Invention
In order to solve the technical problems, the invention provides a composition for relieving physical fatigue and a preparation method thereof, so as to achieve the purposes of improving the utilization rate of protein in jellyfish, and preparing the composition for relieving physical fatigue by compounding jellyfish polypeptide and American ginseng extract.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a composition for relieving physical fatigue comprises jellyfish polypeptides and American ginseng extract, wherein the mass fraction of the jellyfish polypeptides is 80-90%, and the mass fraction of the American ginseng extract is 10-20%; the jellyfish polypeptide is an enzymolysis product of a desalted jellyfish umbrella part or a wrist part.
In the scheme, the protein content in the jellyfish polypeptide is higher than 70%, the polypeptide content in the protein accounts for more than 60% of the total amount, wherein the polypeptide with the molecular weight of more than 5000Da is less than 5%, the polypeptide with the molecular weight of 3000Da < M <5000Da is less than 15%, the polypeptide with the molecular weight of 1000Da < M <3000Da accounts for 25-55%, and the polypeptide with the molecular weight of M <1000Da accounts for 35-60%.
In the above scheme, the preparation method of the jellyfish polypeptide comprises the following steps: taking an umbrella part or a wrist part of a desalted jellyfish, cleaning and homogenizing, taking homogenate, adding water into the homogenate according to a solid-to-liquid ratio of 1:3-6, then adding enzyme for enzymolysis, wherein the enzyme adding amount calculated by the homogenate is 1500-3000U/g, the enzymolysis temperature is 45-65 ℃, the enzymolysis time is 3-8 hours, carrying out enzymolysis under an enzymolysis system with pH of 5-7, and boiling for 10 minutes at 100 ℃ for enzyme deactivation after the enzymolysis is finished; centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
In a further technical scheme, the enzyme is one or a combination of more of papain, bromelain, alkaline protease, neutral protease, flavourzyme, trypsin, chymotrypsin and collagenase.
In the scheme, the polysaccharide content in the American ginseng extract is more than 10 percent, and the saponin content is more than 20 percent.
A preparation method of the composition for relieving physical fatigue comprises the step of uniformly mixing 80-90% of jellyfish polypeptides and 10-20% of American ginseng extracts according to mass fractions.
Through the technical scheme, the composition for relieving physical fatigue and the preparation method thereof have the following beneficial effects:
the composition for relieving physical fatigue provided by the invention combines jellyfish polypeptide of ocean source and American ginseng extract of land source, and has the advantages of good effect, easy absorption, no toxicity and no side effect. In the preparation method of the composition provided by the invention, the desalted jellyfish is directly hydrolyzed into jellyfish polypeptide, so that the utilization rate of jellyfish protein is high, the operation is simple and convenient, and no pollution is caused. The invention improves the value of jellyfish resources from the two aspects of the utilization rate of jellyfish raw materials and the high added value of products.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below.
Preparation of jellyfish polypeptides:
example 1
Taking the umbrella part of the desalted jellyfish, cleaning, homogenizing, taking homogenate, adding water into the homogenate according to a solid-to-liquid ratio of 1:6, then adding papain, wherein the total enzyme adding amount is 2600U/g (calculated by the homogenate), the enzymolysis temperature is 60 ℃, the enzymolysis time is 4 hours, the pH of an enzymolysis system is 6, and boiling is carried out for 10 minutes at 100 ℃ to inactivate enzymes after the enzymolysis is finished. Centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
Example 2
Taking an umbrella part of desalted white jellyfish, cleaning and homogenizing, taking homogenate, adding water into the homogenate according to a solid-to-liquid ratio of 1:3, then adding neutral protease, wherein the total enzyme adding amount is 1500U/g (calculated by the homogenate), the enzymolysis temperature is 55 ℃, the enzymolysis time is 6 hours, the pH of an enzymolysis system is 7, and boiling is carried out for 10 minutes at 100 ℃ to inactivate enzyme after the enzymolysis is finished. Centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
Example 3
Taking the umbrella part of the desalted macular jellyfish, cleaning, homogenizing, taking the homogenate, adding water according to the solid-to-liquid ratio of 1:3, then adding bromelain, wherein the total enzyme adding amount is 2000U/g (calculated by the homogenate), the enzymolysis temperature is 50 ℃, the enzymolysis time is 8 hours, the pH of an enzymolysis system is 7, and boiling for 10 minutes at 100 ℃ to inactivate enzyme after the enzymolysis is finished. Centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
Example 4
Taking an umbrella part of desalted jellyfish, cleaning the umbrella part, homogenizing, taking homogenate, adding water into the homogenate according to a solid-to-liquid ratio of 1:4, then adding bromelain and trypsin, wherein the total amount of the added enzyme is 2200U/g (calculated by the homogenate), 1000U/g of bromelain and 1200U/g of trypsin are added, the enzymolysis temperature is 50 ℃, the enzymolysis time is 3 hours, the pH of an enzymolysis system is 6, and boiling is carried out for 10 minutes at 100 ℃ after the enzymolysis is finished to inactivate enzyme. Centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
Example 5
Taking an umbrella part of desalted white jellyfish, cleaning and homogenizing, adding water into the homogenate according to a solid-to-liquid ratio of 1:3, and then adding chymotrypsin and alkaline protease, wherein the total amount of the added enzymes is 2000U/g (calculated by the homogenate), 1000U/g of chymotrypsin, 1000U/g of alkaline protease and an enzymolysis temperature of 50 ℃, an enzymolysis time of 5 hours, an enzymolysis system pH 6, and boiling for 10 minutes at 100 ℃ after the enzymolysis is finished to inactivate enzymes. Centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
Example 6
Taking the umbrella part of the desalted macular jellyfish, cleaning, homogenizing, taking the homogenate, adding water according to the solid-to-liquid ratio of 1:3, then adding flavourzyme and collagenase, wherein the total amount of the added enzymes is 1800U/g (calculated by the homogenate), 1000U/g of flavourzyme, 800U/g of collagenase, the enzymolysis temperature is 60 ℃, the enzymolysis time is 3.5 hours, the pH of an enzymolysis system is 6.5, and boiling for 10 minutes at 100 ℃ to inactivate enzymes after the enzymolysis is finished. Centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide.
In the above examples, the conditions of centrifugation, concentration and lyophilization are not limited, and may be according to a method conventional in the art.
The protein content, the polypeptide content and the molecular weight distribution of jellyfish polypeptides were determined according to the method in national standard GB/T22492 (soybean peptide powder), and the results are shown in Table 1.
TABLE 1 jellyfish polypeptide protein content, polypeptide content and molecular weight distribution
Preparation of the composition:
the jellyfish polypeptides and American ginseng extracts prepared in the above examples were uniformly mixed as shown in Table 2 to obtain a composition. The method of mixing the composition of the present invention is not limited and may be according to a method conventional in the art.
The extraction method of the American ginseng extract is not particularly limited, and the American ginseng extract can be prepared according to a conventional method in the art, for example, crushing the root of American ginseng, adding ethanol for extraction, or extracting with hot water, filtering to remove residues, concentrating the supernatant, and spray-drying or freeze-drying. Or commercially available. The method for measuring the content of polysaccharide and saponin in the American ginseng extract is not particularly limited, and the American ginseng extract can be obtained by testing according to the conventional means in the field, such as spectrophotometry, high performance liquid chromatography and the like. The American ginseng extract used in the invention has the polysaccharide content of 15 percent and the saponin content of 26 percent.
Table 2 preparation examples and comparative examples of compositions
Of the above-mentioned compositions, compositions 1 to 6 are examples within the range defined in the present invention, and compositions 7 to 9 are comparative examples outside the range defined in the present invention.
Evaluation of physical fatigue relieving efficacy:
1. weight bearing swimming experiment
1.1 test article
Compositions 1-9 were prepared.
1.2 laboratory animals
The number of Kunming mice was 48, the body weight of male mice was 18-22 g, and the mice were randomly divided into 4 groups of 12 mice each.
1.3 dose design and grouping
The recommended dosage of the test substance is 2.1g/d for adults (calculated by 60kg of body weight), and the mice are subjected to gastric administration according to 350mg/kg.d which is 10 times of the recommended dosage of human body, and the empty control group is filled with gastric distilled water, the administration frequency is 1 time/day, and the administration time is 30 days.
1.4 Experimental procedure
After the sample is given for 30min last time, the tail root of the mice loaded with 5% weight lead skin is placed in a swimming box for swimming. The water depth was not less than 30cm, the water temperature was 25.+ -. 1.0 ℃, and the time from the start of the swimming of the mice to death, i.e., the load swimming time of the mice, was recorded, and the results are shown in Table 3.
Table 3 effect of each composition on the swimming time of mice (n=10)
* Representing examples versus controls, p<0.05, with significant differences; # indicating that the examples compare with the comparative examples, p<0.05, with significant differences.
2. Serum urea assay
2.1 test article
Composition 1-composition 9 was prepared.
2.2 laboratory animals
The number of Kunming mice was 48, the body weight of male mice was 18-22 g, and the mice were randomly divided into 4 groups of 12 mice each.
2.3 dose design and grouping
The recommended dosage of the test substance is 2.1g/d for adults (calculated by 60kg of body weight), and the mice are subjected to gastric administration according to 350mg/kg.d which is 10 times of the recommended dosage of human body, and the empty control group is filled with gastric distilled water, the administration frequency is 1 time/day, and the administration time is 30 days.
2.4 Experimental methods
After the sample is given for 30min at last, the sample is swim for 90min in water with the temperature of 30 ℃ without load, and blood is taken after resting for 60 min. Mice were drawn to eye and whole blood was collected at about 0.5mL (no anticoagulant added). Placing in a refrigerator at 4deg.C for about 3 hr, centrifuging at 2000rpm/min for 15min after blood coagulation, and collecting serum. Urea in serum can be stabilized for 24h at room temperature and for more than 7 days at 4-6 ℃. Serum urea content was determined by diacetyl-oxime method.
3 liver glycogen determination
3.1 test article
Composition 1-composition 9 was prepared.
3.2 laboratory animals
The number of Kunming mice was 48, the body weight of male mice was 18-22 g, and the mice were randomly divided into 4 groups of 12 mice each.
3.3 dose design and grouping
The recommended dosage of the test substance is 2.1g/d for adults (calculated by 60kg of body weight), and the mice are subjected to gastric administration according to 350mg/kg.d which is 10 times of the recommended dosage of human body, and the empty control group is filled with gastric distilled water, the administration frequency is 1 time/day, and the administration time is 30 days.
3.4 Experimental methods
Animals were sacrificed 30min after the last sample, livers were removed, and hepatic glycogen was measured by anthrone method.
4 lactic acid assay
4.1 test article
Composition 1-composition 9 was prepared.
4.2 laboratory animals
The number of Kunming mice was 48, the body weight of male mice was 18-22 g, and the mice were randomly divided into 4 groups of 12 mice each.
4.3 dose design and grouping
The recommended dosage of the test substance is 2.1g/d for adults (calculated by 60kg of body weight), and the mice are subjected to gastric administration according to 350mg/kg.d which is 10 times of the recommended dosage of human body, and the empty control group is filled with gastric distilled water, the administration frequency is 1 time/day, and the administration time is 30 days.
4.4 Experimental methods
The sample to be tested is sampled for 30min at last, and then swimming in water with the temperature of 30 ℃ for 10min without load, and stopping. Before swimming, 20 mu L of blood is taken and added into 40 mu L of membrane rupture liquid, and the broken cells are fully oscillated immediately; immediately taking 20 mu L of blood after swimming, adding the blood into 40 mu L of membrane rupture liquid, and immediately and fully oscillating and crushing cells; after resting for 20min, 20 μl of each blood was collected and added to 40 μl of the membrane-broken solution, and the mixture was measured by a lactate meter. Mice were collected from the inner canthus using capillaries.
The results of the above experiments are shown in Table 4.
Table 4 effect of each composition on mouse serum urea BUN, liver glycogen, blood lactate LA (n=10)
* Representing examples versus controls, p<0.05, with significant differences; # indicating that the examples compare with the comparative examples, p<0.05, with significant differences.
Judging the physical fatigue relieving function:
the test result of the load swimming is positive, and any two of three biochemical indexes of blood lactic acid, serum urea and hepatic glycogen are positive, so that the tested sample can be judged to have the function of relieving physical fatigue. As can be seen from tables 3 and 4, compared with the blank control group, the product of the invention can obviously improve the mouse load swimming time, improve the liver glycogen content, reduce the serum urea and blood lactic acid content, and the p is less than 0.05, and compared with the comparative example composition 7-9 beyond the range defined by the invention, the product of the invention can also obviously improve the mouse load swimming time, improve the liver glycogen content, reduce the serum urea and blood lactic acid content, and shows that the composition formed within the protection range defined by the invention has the effect of relieving physical fatigue.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (4)
1. The composition for relieving physical fatigue is characterized by comprising 80-90% of jellyfish polypeptide and 10-20% of American ginseng extract; the jellyfish polypeptide is an enzymolysis product of a desalted jellyfish umbrella part or a wrist part;
the preparation method of the jellyfish polypeptide comprises the following steps: taking an umbrella part or a wrist part of a desalted jellyfish, cleaning and homogenizing, taking homogenate, adding water into the homogenate according to a solid-to-liquid ratio of 1:3-6, then adding enzyme for enzymolysis, wherein the enzyme adding amount calculated by the homogenate is 1500-3000U/g, the enzymolysis temperature is 45-65 ℃, the enzymolysis time is 3-8 hours, carrying out enzymolysis under an enzymolysis system with pH of 5-7, and boiling for 10 minutes at 100 ℃ for enzyme deactivation after the enzymolysis is finished; centrifuging, removing residues, concentrating the supernatant by rotary evaporation to 1/4-1/5 of the initial volume, and freeze-drying to obtain jellyfish polypeptide;
the polysaccharide content in the American ginseng extract is more than 10 percent, and the saponin content is more than 20 percent.
2. The composition for alleviating physical fatigue according to claim 1, wherein the jellyfish polypeptide has a protein content of more than 70%, the protein content of more than 60% of the total amount, wherein the polypeptide having a molecular weight of >5000Da is less than 5%, the polypeptide having a molecular weight of 3000Da < M <5000Da is less than 15%, the polypeptide having a molecular weight of 1000Da < M <3000Da is 25-55%, and the polypeptide having a molecular weight of M <1000Da is 35-60%.
3. The composition for alleviating physical fatigue according to claim 1, wherein the enzyme is one or a combination of papain, bromelain, alkaline protease, neutral protease, flavourzyme, trypsin, chymotrypsin, collagenase.
4. The method for preparing the composition for relieving physical fatigue according to claim 1, wherein jellyfish polypeptide is uniformly mixed according to the mass fraction of 80-90% and American ginseng extract is uniformly mixed according to the mass fraction of 10-20%, so as to obtain the composition.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101004539B1 (en) * | 2010-08-13 | 2010-12-31 | 이자빈 | Fermentation composition using jellyfish. and the manufacturing method thereof |
| CN103783609A (en) * | 2013-12-20 | 2014-05-14 | 芜湖金鹰机械科技开发有限公司 | Ginseng health-care beverage and production method thereof |
| CN105995842A (en) * | 2016-06-29 | 2016-10-12 | 马昱 | Jellyfish-pseudo-ginseng soup formula |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101004539B1 (en) * | 2010-08-13 | 2010-12-31 | 이자빈 | Fermentation composition using jellyfish. and the manufacturing method thereof |
| CN103783609A (en) * | 2013-12-20 | 2014-05-14 | 芜湖金鹰机械科技开发有限公司 | Ginseng health-care beverage and production method thereof |
| CN105995842A (en) * | 2016-06-29 | 2016-10-12 | 马昱 | Jellyfish-pseudo-ginseng soup formula |
Non-Patent Citations (2)
| Title |
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| Natural medicines for the treatment of fatigue: Bioactive components, pharmacology, and mechanisms;Chuanhong Luo;Natural medicines for the treatment of fatigue: Bioactive components, pharmacology, and mechanisms(第148期);1-22 * |
| 海洋生物毒素研究新进展;邴晖;海洋生物毒素研究新进展;第29卷(第1期);78-85 * |
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