CN103493885B - Yoghurt with sea cucumber polypeptide and processing method for yoghurt - Google Patents
Yoghurt with sea cucumber polypeptide and processing method for yoghurt Download PDFInfo
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- CN103493885B CN103493885B CN201310482021.7A CN201310482021A CN103493885B CN 103493885 B CN103493885 B CN 103493885B CN 201310482021 A CN201310482021 A CN 201310482021A CN 103493885 B CN103493885 B CN 103493885B
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Abstract
The invention relates to preparation of fermented milk with additives, in particular to yoghurt with sea cucumber polypeptide and a processing method for the yoghurt. The yoghurt with the sea cucumber polypeptide comprises the following materials in percent by weight: 5-15% of sea cucumber polypeptide and 85-95% of yoghurt. The processing method comprises the following steps of using pre-treated sea cucumber internal organs as raw materials; adding distilled water of which the weight is 4-8 times the weight of the raw materials in the raw material; uniformly stirring the mixture to obtain homogenate; adjusting the pH (potential of hydrogen) value of the homogenate to be 5.8-6.8; controlling the temperature of the homogenate to be 35-45 DEG C; adding bromelain and trypsin in the mixture; staying warm for 2-4 hours; then performing enzyme deactivation; centrifuging the mixture; taking supernate; performing decoloration and deodorization on the supernate by using activated carbon; filtering the supernate by using an ultrafiltration membrane to obtain sea cucumber internal organ polypeptide with the molecular weight lower than 5000 Dalton; and mixing the sea cucumber internal organ polypeptide and the yoghurt to obtain a finished product. The yoghurt with the sea cucumber polypeptide is rich in nutrition, easy to absorb and convenient to drink, tastes good, has effects of oxidation resistance, aging resistance and the like, is reasonable in technology and high in operability, and facilitates industrial production.
Description
Technical field
The present invention relates to the fermented milk preparation that contains additive, is a kind of sea cucumber polypeptide yoghurt and preparation method thereof specifically.
Background technology
Sea cucumber has very high nutrition and medical value, and before the centuries, the just seafood delights using sea cucumber as a kind of preciousness of China people, classifies one of marine products " eight delicacies " as.Along with the generally reinforcement of people's health care consciousness, rapidly, production scale and market scale constantly expand in its aquaculture industry development in recent years, and still incident is in process, to produce the accessory substances such as a large amount of internal organ (intestines, ovum).China is nowhere near to the utilization of this accessory substance at present, and most of enterprise is abandoned, and causes the waste of environmental pollution and resource.Research finds that sea cucumber internal organ contain same rich in protein with body wall, but the value of current this protein does not fully demonstrate out.
Make full use of Holothurian machining leftover bits and pieces and can greatly improve sea cucumber comprehensive utilization degree.Chinese invention patent CN1947587A discloses a kind of leftover bits and pieces-sea cucumber cooking water in existing food of sea cucumber process, sea cucumber internal organ and has made the method for soy sauce as raw material.Chinese invention patent CN102757861A discloses a kind of method that adopts subcritical R134a to prepare sea cucumber fat from sea cucumber internal organ.Chinese invention patent CN102605031A discloses a kind of sea cucumber internal organ and has utilized method, by pretreatment of raw material, alkali carry, enzymolysis, centrifugation, micro-filtration membrane removal of impurities, NF membrane are concentrated, alcohol precipitation, centrifugation, dry sea cucumber polysaccharide and the sea cucumber polypeptide of making.It is an approach that utilizes this Holothurian machining leftover bits and pieces that sea cucumber internal organ enzymolysis is prepared to polypeptide.But the fishy smell of the polypeptide being made by internal organ is more much bigger than what make, greatly limited the application of internal organ polypeptide from the raw materials of marine products such as fish-skin, fish scale.Now fish-skin, the fish scale polypeptide on market is collagen polypeptide, made by collagen, and sea cucumber internal organ polypeptide to be internal organ total protease solution obtain, with collagen polypeptide be discrepant, but the product of internal organ polypeptide is also little at present.
Yoghurt is taking milk as raw material, adds appropriate granulated sugar, through pasteurize and cooling after, add lactic acid bacteria fermenting agent, the product of making through heat-preservation fermentation.Glucose, fructose, galactolipin and lactose in sweat in milk can ferment and generate lactic acid and other a small amount of materials.These variations not only give Yoghourt special local flavor, and make Yoghourt than fresh milk absorption more easy to digest.The health-care effect highly significant of Yoghourt, mainly contains: regulate gastrointestinal bacterial flora, improve body immune system, suppress tumour, improve the dysbolism of lactose intolerance, reduce cholesterol levels, suppress vivotoxin, delay body aging.
Have no up to now and add sea cucumber internal organ polypeptide to research report in Yoghourt.
Summary of the invention
The object of the invention is to provide a kind of sea cucumber polypeptide yoghurt and preparation method thereof.
For achieving the above object, the present invention adopts technical scheme to be:
A kind of sea cucumber polypeptide yoghurt: by weight percentage, in sea cucumber polypeptide yoghurt, sea cucumber polypeptide is 5～15%, and yoghurt is 85～95%.
Described sea cucumber polypeptide is, taking pretreated sea cucumber internal organ as raw material, in raw material, add the distilled water of 4～8 times of raw material weights to blend homogenized and obtain homogenate, and to regulate pH value be 5.8～6.8, controlling temperature is 35～45 DEG C, simultaneously to the bromelain and the trypsase that add 6000～8000 enzyme activity units (U) in every gram of homogenate, be incubated 2～4 hours, enzyme then goes out, centrifuging and taking supernatant, supernatant is again through ultrafiltration membrance filter after activated carbon decolorizing defishying, and obtaining molecular weight is the sea cucumber internal organ polypeptide liquid below 5000 dalton.
Described bromelain and trypsase, the two enzyme activity proportioning is 1:1～1:3.
Described molecular weight be sea cucumber internal organ polypeptide liquid below 5000 dalton under the condition of 115～121 DEG C, sterilizing 10～20 minutes, stand-by.
A kind of preparation method of sea cucumber polypeptide yoghurt:
1) enzymolysis: taking pretreated sea cucumber internal organ as raw material, in raw material, add the distilled water of 4～8 times of its weight to mix to obtain homogenate, and to regulate pH value be 5.8～6.8, controlling temperature is 35～45 DEG C, simultaneously to the bromelain and the trypsase that add 6000～8000 enzyme activity units (U) in every gram of homogenate, be incubated 2～4 hours, obtain sea cucumber internal organ enzymolysis liquid;
2) enzyme that goes out: the above-mentioned sea cucumber internal organ enzymolysis liquid making is heated to 90～100 DEG C, keeps 10～20 minutes, enzyme goes out;
3) centrifugation: by step 2) gained enzymolysis liquid is cooled to after room temperature 4000r/min centrifugal 20～30 minutes, removes macro-molecular protein and insoluble magazine, gets supernatant;
4) decoloration deodorization: add 80 object powdered activated carbons in above-mentioned supernatant, making its final percentage composition is 1.0～2.0%, decoloration deodorization 10～30 minutes under the condition of 25～35 DEG C and pH4.0～6.0, filters and collects filtrate;
5) ultrafiltration: above-mentioned filtrate is adopted to ultrafiltration membrance filter, filter to such an extent that molecular weight is the sea cucumber internal organ polypeptide liquid below 5000 dalton;
6) sterilizing: by above-mentioned gained polypeptide liquid under the condition of 115～121 DEG C, sterilizing 10～20 minutes;
7) mix and blend: the sea cucumber internal organ polypeptide liquid after above-mentioned sterilizing is mixed, stirred with Yoghourt, and its mixed polypeptide liquid percentage by weight is 5～15%, and yoghurt is 85～95%.
Described bromelain and trypsase, the two enzyme activity proportioning is 1:1～1:3.
Described pretreated sea cucumber internal organ are that sea cucumber internal organ are removed to silt, clean up, and drain away the water stand-by.
Yoghurt after mixing and sea cucumber internal organ polypeptide liquid are carried out to sterile filling, sea cucumber polypeptide yoghurt.
The present invention has advantages of:
1. the present invention adopts the sea cucumber internal organ polypeptide liquid of molecular weight, and it not only has certain physiologically active, and absorption easy to digest.But in the process of proteolysis, along with the exposure of hydrophobic amino acid, can produce bitter taste, polypeptide itself has certain fishy smell simultaneously, and the present invention adds sea cucumber internal organ polypeptide in yoghurt to, alleviates the bitter off-flavors of polypeptide, increase the designs and varieties of Yoghourt simultaneously, and with low cost.
2. sea cucumber polypeptide Yoghourt of the present invention is nutritious, easily absorbs, and drinks conveniently, and good mouthfeel, has anti-oxidant, the anti-ageing effect of waiting for a long time.
3. polypeptide used in the present invention, raw material, from processing of aquatic products discarded object, makes full use of processing of aquatic products discarded object, not only can reduce the waste of environmental pollution and resource, and can increase the added value of processing of aquatic products, increases economic efficiency.
4. technique of the present invention is simple, easy to operate, with low cost, and economical and effective, easily realizes suitability for industrialized production.
Detailed description of the invention
Embodiment 1
Sea cucumber internal organ are removed to silt, clean up, drain away the water, get 10kg, with the homogenate of 40kg distilled water, mix, adjust pH to 5.8, is under the condition of 35 DEG C in temperature, be that 1:1 adds bromelain and trypsase according to enzyme activity proportioning, total enzyme concentration is 6000 enzyme activity units, and enzymolysis was heated to enzymolysis liquid 90 DEG C after 2 hours, kept 10 minutes enzymes that go out; The enzyme that goes out enzymolysis liquid after treatment is removed macro-molecular protein and insoluble impurities for centrifugal 20 minutes under the condition of 4000r/min after being cooled to room temperature; Get supernatant, add a certain amount of 80 object powdered activated carbons, making its final percentage composition is 1.5%, decoloration deodorization 20 minutes under the condition of 25 DEG C and pH4.0, filter and collect filtrate, then carry out ultrafiltration by the daltonian ultrafiltration membrane filter device of molecular cut off 5000, filter to such an extent that molecular weight is the sea cucumber internal organ polypeptide enzymolysis liquid 45kg below 5000 dalton, the sterilizing 20 minutes under 120 DEG C of conditions of this polypeptide liquid; Get the yoghurt 855kg preparing according to conventional method and mix with sea cucumber internal organ polypeptide liquid, carry out sterile filling, every tank 200mL, sea cucumber polypeptide yoghurt.
Embodiment 2
Sea cucumber internal organ are removed to silt, clean up, drain away the water, get 20kg, with the homogenate of 100kg distilled water, mix, adjust pH to 6.3, is under the condition of 40 DEG C in temperature, be that 1:3 adds bromelain and trypsase according to enzyme activity proportioning, total enzyme concentration is 7000 enzyme activity units, and enzymolysis was heated to enzymolysis liquid 100 DEG C after 3 hours, kept 10 minutes enzymes that go out; Enzymolysis liquid is removed macro-molecular protein and insoluble impurities for centrifugal 30 minutes under the condition of 4000r/min after being cooled to room temperature; Get supernatant, add a certain amount of 80 object powdered activated carbons, making its final percentage composition is 1.0%, decoloration deodorization 20 minutes under the condition of 35 DEG C and pH5.0, filter and collect filtrate, then carry out ultrafiltration by the daltonian ultrafiltration membrane filter device of molecular cut off 5000, filter to such an extent that molecular weight is the sea cucumber internal organ polypeptide enzymolysis liquid 115kg below 5000 dalton, the sterilizing 20 minutes under 115 DEG C of conditions of this polypeptide liquid; Get yoghurt 1150kg prepared by conventional method and mix with sea cucumber internal organ polypeptide liquid, carry out sterile filling, every tank 200mL, sea cucumber polypeptide yoghurt.
Embodiment 3
Sea cucumber internal organ are removed to silt, clean up, drain away the water, get 10kg, with the homogenate of 80kg distilled water, mix, adjust pH to 5.8, is under the condition of 45 DEG C in temperature, be that 1:2 adds bromelain and trypsase according to enzyme activity proportioning, total enzyme concentration is 8000 enzyme activity units, and enzymolysis was heated to enzymolysis liquid 95 DEG C after 3 hours, kept 20 minutes enzymes that go out; Enzymolysis liquid is removed macro-molecular protein and insoluble impurities for centrifugal 20 minutes under the condition of 4000r/min after being cooled to room temperature; Get supernatant, add a certain amount of 80 object powdered activated carbons, making its final percentage composition is 1.5%, decoloration deodorization 10 minutes under the condition of 30 DEG C and pH5.0, filter and collect filtrate, then carry out ultrafiltration by the daltonian ultrafiltration membrane filter device of molecular cut off 5000, filter to such an extent that molecular weight is the sea cucumber internal organ polypeptide enzymolysis liquid 80kg below 5000 dalton, the sterilizing 10 minutes under 121 DEG C of conditions of this polypeptide liquid; Get yoghurt 456kg prepared by conventional method and mix with sea cucumber internal organ polypeptide liquid, carry out sterile filling, every tank 100mL, sea cucumber polypeptide yoghurt.
Embodiment 4
Sea cucumber internal organ are removed to silt, clean up, drain away the water, get 30kg, with the homogenate of 180kg distilled water, mix, adjust pH to 6.8, is under the condition of 35 DEG C in temperature, be that 1:2 adds bromelain and trypsase according to enzyme activity proportioning, total enzyme concentration is 6000 enzyme activity units, and enzymolysis was heated to enzymolysis liquid 100 DEG C after 4 hours, kept 15 minutes enzymes that go out; Enzymolysis liquid is removed macro-molecular protein and insoluble impurities for centrifugal 30 minutes under the condition of 4000r/min after being cooled to room temperature; Get supernatant, add a certain amount of 80 object powdered activated carbons, making its final percentage composition is 2.0%, decoloration deodorization 30 minutes under the condition of 30 DEG C and pH6.0, filter and collect filtrate, then carry out ultrafiltration by the daltonian ultrafiltration membrane filter device of molecular cut off 5000, filter to such an extent that molecular weight is the sea cucumber internal organ polypeptide enzymolysis liquid 200kg below 5000 dalton, the sterilizing 15 minutes under 120 DEG C of conditions of this polypeptide liquid; Get yoghurt 3000kg prepared by conventional method and mix with sea cucumber internal organ polypeptide liquid, carry out sterile filling, every tank 150mL, sea cucumber polypeptide yoghurt.
Above-described embodiment is obtained to enzymolysis liquid and carries out the mensuration of antioxidation activity:
1. the scavenging action of enzymolysis liquid to hydroxy radical
In scale test tube, add the HAc-NaAc buffer solution (pH5.0) of 0.8mL, the malachite green solution (1 × 10 of 1.0mL
-4mol/L), the EDTA-Fe of 2.2mL
2+solution (2 × 10
-2mol/L), the H of 1.4mL volume fraction 0.3%
2o
2solution, is diluted to 10mL and shakes up by deionized water, measures absorbance A after placing 45min under 617nm
b, measure and do not add H simultaneously
2o
2time the absorbance A of system under 617nm
0, the generation of hydroxy radical is △ A=A
0-A
b.△ A is larger, represents that the hydroxy radical generating is more.
In above-mentioned system, add H
2o
2add the to be measured enzymolysis liquid sample of 1mL through the above-mentioned example of 8 times of dilutions before, measure its absorbance A
s, the clearance rate of hydroxy radical (Scavenging rate of hydroxyl radical, SRHR) is:
2. the scavenging action of enzymolysis liquid to ultra-oxygen anion free radical
Get Tris-HCl buffer solution (pH8.2, the 2mmol/L Na of 50mmol/L
2eDTA) 3mL, add the above-mentioned example of 0.5mL enzyme liquid to be measured, in 25 DEG C of insulation 10min, then add pyrogallol (in advance with the 10mmol/L HCl preparation) 0.1mL of the 5mmol/L of 25 DEG C of pre-temperature, reaction 4min, the absorbance of solution of every 30s mensuration under 320nm, calculates pyrogallol autoxidation speed, returns to zero as blank using 10mmol/L HCl.Calculate according to the following formula the clearance rate (Scavenging rate of superoxide anion radical, SRSAR) of superoxide anion.
V
0: do not add pyrogallol autoxidation speed in the reactant liquor of testing sample.
V
s: add pyrogallol autoxidation speed in the reactant liquor of testing sample.
Through said determination, the clearance rate to hydroxy radical and ultra-oxygen anion free radical of above-mentioned enzymolysis liquid is respectively 49.65% and 52.66%.
Claims (7)
1. a sea cucumber polypeptide yoghurt, is characterized in that: by weight percentage, in sea cucumber polypeptide yoghurt, sea cucumber polypeptide is 5～15%, and yoghurt is 85～95%;
Described sea cucumber polypeptide is, taking pretreated sea cucumber internal organ as raw material, in raw material, add the distilled water of 4～8 times of raw material weights to obtain homogenate, and to regulate pH value be 5.8～6.8, controlling temperature is 35～45 DEG C, simultaneously to the bromelain and the trypsase that add 6000～8000 enzyme activity units (U) in every gram of homogenate, be incubated 2～4 hours, enzyme then goes out, centrifuging and taking supernatant, supernatant is again through ultrafiltration membrance filter after activated carbon decolorizing defishying, and obtaining molecular weight is the sea cucumber internal organ polypeptide liquid below 5000 dalton.
2. by sea cucumber polypeptide yoghurt claimed in claim 1, it is characterized in that: described bromelain and trypsase, the two enzyme activity proportioning is 1:1～1:3.
3. by sea cucumber polypeptide yoghurt claimed in claim 1, it is characterized in that: described molecular weight be sea cucumber internal organ polypeptide liquid below 5000 dalton under the condition of 115～121 DEG C, sterilizing 10～20 minutes, stand-by.
4. a preparation method for sea cucumber polypeptide yoghurt claimed in claim 1, is characterized in that:
1) enzymolysis: taking pretreated sea cucumber internal organ as raw material, in raw material, add the distilled water of 4～8 times of its weight to mix to obtain homogenate, and to regulate pH value be 5.8～6.8, controlling temperature is 35～45 DEG C, simultaneously to the bromelain and the trypsase that add 6000～8000 enzyme activity units (U) in every gram of homogenate, be incubated 2～4 hours, obtain sea cucumber internal organ enzymolysis liquid;
2) enzyme that goes out: the above-mentioned sea cucumber internal organ enzymolysis liquid making is heated to 90～100 DEG C, keeps 10～20 minutes, enzyme goes out;
3) centrifugation: by step 2) gained enzymolysis liquid is cooled to after room temperature 4000r/min centrifugal 20～30 minutes, removes macro-molecular protein and insoluble impurities, gets supernatant;
4) decoloration deodorization: add 80 object powdered activated carbons in above-mentioned supernatant, making its final percentage composition is 1.0～2.0%, decoloration deodorization 10～30 minutes under the condition of 25～35 DEG C and pH4.0～6.0, filters and collects filtrate;
5) ultrafiltration: above-mentioned filtrate is adopted to ultrafiltration membrance filter, filter to such an extent that molecular weight is the sea cucumber internal organ polypeptide liquid below 5000 dalton;
6) sterilizing: by above-mentioned gained polypeptide liquid under the condition of 115～121 DEG C, sterilizing 10～20 minutes;
7) mix and blend: the sea cucumber internal organ polypeptide liquid after above-mentioned sterilizing is mixed, stirred with Yoghourt, and its mixed polypeptide liquid percentage by weight is 5～15%, and yoghurt is 85～95%.
5. by the preparation method of sea cucumber polypeptide yoghurt claimed in claim 4, it is characterized in that: described bromelain and trypsase, the two enzyme activity proportioning is 1:1～1:3.
6. by the preparation method of sea cucumber polypeptide yoghurt claimed in claim 4, it is characterized in that: described pretreated sea cucumber internal organ, for sea cucumber internal organ are removed to silt, clean up, and drain away the water stand-by.
7. by the preparation method of sea cucumber polypeptide yoghurt claimed in claim 4, it is characterized in that: the yoghurt after mixing and sea cucumber internal organ polypeptide liquid are carried out to sterile filling, sea cucumber polypeptide yoghurt.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103919169B (en) * | 2014-04-30 | 2015-08-12 | 烟台新海水产食品有限公司 | A kind of method utilizing sea cucumber to produce health products |
| CN104663891B (en) * | 2015-02-03 | 2017-09-22 | 湖南小洋人科技发展有限公司 | A kind of preparation method of the additive of stirred yoghurt |
| CN105211284A (en) * | 2015-08-20 | 2016-01-06 | 谭萍 | A kind of sea cucumber boruga and preparation method thereof |
| CN105982328A (en) * | 2016-01-07 | 2016-10-05 | 安徽省华银茶油有限公司 | Trepang nourishing tea leaf seed polypeptide lozenge and preparation method thereof |
Citations (2)
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|---|---|---|---|---|
| CN101209071A (en) * | 2006-12-25 | 2008-07-02 | 邓洁 | Milk containing fresh sea cucumber (sea cucumber polypeptide, sea cucumber polysaccharide) processed by low temperature ultra-high pressure freeze-drying micropowder technique and preparation method t |
| CN101356984A (en) * | 2007-07-31 | 2009-02-04 | 邓洁 | High-calcium sea-slug (sea-slug peptide, sea-slug polysaccharide) milk and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101209071A (en) * | 2006-12-25 | 2008-07-02 | 邓洁 | Milk containing fresh sea cucumber (sea cucumber polypeptide, sea cucumber polysaccharide) processed by low temperature ultra-high pressure freeze-drying micropowder technique and preparation method t |
| CN101356984A (en) * | 2007-07-31 | 2009-02-04 | 邓洁 | High-calcium sea-slug (sea-slug peptide, sea-slug polysaccharide) milk and preparation method thereof |
Non-Patent Citations (2)
| Title |
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| 海棒槌胶原蛋白的物理特性及其酶解物的生物活性研究;肖枫;《中国优秀硕士论文数据库》;20060115;第23页第2段、表3-1，第31页第1、2段 * |
| 肖枫.海棒槌胶原蛋白的物理特性及其酶解物的生物活性研究.《中国优秀硕士论文数据库》.2006,第23页第2段、表3-1，第31页第1、2段. * |
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