CN105982123A - Preparation method of earthworm protein beverage and product obtained by preparation method - Google Patents
Preparation method of earthworm protein beverage and product obtained by preparation method Download PDFInfo
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- CN105982123A CN105982123A CN201510842382.7A CN201510842382A CN105982123A CN 105982123 A CN105982123 A CN 105982123A CN 201510842382 A CN201510842382 A CN 201510842382A CN 105982123 A CN105982123 A CN 105982123A
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- 241000361919 Metaphire sieboldi Species 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 235000021568 protein beverage Nutrition 0.000 title claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 10
- 108091005804 Peptidases Proteins 0.000 claims abstract description 9
- 239000004365 Protease Substances 0.000 claims abstract description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 5
- 102000004142 Trypsin Human genes 0.000 claims abstract description 5
- 108090000631 Trypsin Proteins 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- 239000012588 trypsin Substances 0.000 claims abstract description 5
- 241000243684 Lumbricus Species 0.000 claims description 40
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- 230000002255 enzymatic effect Effects 0.000 claims description 17
- 239000000413 hydrolysate Substances 0.000 claims description 17
- 241000195474 Sargassum Species 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 239000000796 flavoring agent Substances 0.000 claims description 9
- 235000019634 flavors Nutrition 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000001976 enzyme digestion Methods 0.000 claims description 4
- 238000005360 mashing Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000009928 pasteurization Methods 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000003828 vacuum filtration Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 235000019640 taste Nutrition 0.000 abstract description 6
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 229920000858 Cyclodextrin Polymers 0.000 abstract 1
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 abstract 1
- 235000011175 beta-cyclodextrine Nutrition 0.000 abstract 1
- 239000001202 beta-cyclodextrine Substances 0.000 abstract 1
- 235000009508 confectionery Nutrition 0.000 abstract 1
- 230000009849 deactivation Effects 0.000 abstract 1
- 241001233061 earthworms Species 0.000 abstract 1
- 230000008030 elimination Effects 0.000 abstract 1
- 238000003379 elimination reaction Methods 0.000 abstract 1
- 238000000265 homogenisation Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 235000013361 beverage Nutrition 0.000 description 10
- 241000237636 Pheretima Species 0.000 description 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- FLNXBVJLPJNOSI-UHFFFAOYSA-N 1-[2-[(4-chlorophenyl)-phenylmethoxy]ethyl]piperidine Chemical compound C1=CC(Cl)=CC=C1C(C=1C=CC=CC=1)OCCN1CCCCC1 FLNXBVJLPJNOSI-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 206010019468 Hemiplegia Diseases 0.000 description 1
- 208000007101 Muscle Cramp Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001387 anti-histamine Effects 0.000 description 1
- 239000000739 antihistaminic agent Substances 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000000812 cholinergic antagonist Substances 0.000 description 1
- 229960002544 cloperastine Drugs 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003172 expectorant agent Substances 0.000 description 1
- 230000003419 expectorant effect Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- SBNFWQZLDJGRLK-UHFFFAOYSA-N phenothrin Chemical compound CC1(C)C(C=C(C)C)C1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 SBNFWQZLDJGRLK-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000002048 spasmolytic effect Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/80—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention provides a preparation method of an earthworm protein beverage and a product obtained by the preparation method. The preparation method comprises the following steps of selecting earthworms as raw materials, performing homogenization, performing enzymolysis with double enzymes of flavored protease and trypsin, performing enzyme deactivation, adding yeast powder, performing uniform stirring, performing standing for fishy smell elimination, adding beta-cyclodextrine, performing stirring, performing debitterizing treatment, adding activated carbon for performing decoloration treatment, and performing sucking filtration so as to obtain transparent earthworm enzymolysis protein liquid free from peculiar smell; and performing blending, and performing sterilization so as to obtain the earthworm protein beverage. According to the preparation method disclosed by the invention, the nutrient component of the earthworm enzymolysis protein liquid and the characteristic that the earthworm enzymolysis protein liquid is easy to absorb are utilized, and the inherent fishy taste of the earthworm enzymolysis protein liquid is eliminated, so that the earthworm protein beverage which is rich in nutrition, clear, transparent, and moderate in sour and sweet degrees is made. The preparation method is reasonable in technology, high in operability and suitable for large-scale production.
Description
Technical field
The present invention relates to preparation method and the products obtained therefrom of a kind of earthworm protein beverage, belong to beverage production field.
Background technology
Lumbricus, Chinese medicine claims Pheretima, and its salty in the mouth is cold in nature, and function is heat clearing away, spasmolytic, Zhichuan, diuresis.Cure mainly high fanatical hot-tempered, convulsion with spasms, headache conjunctival congestion, the diseases such as expectorant heat, apoplexy, hemiplegia of panting.Pheretima extracting solution has good determines the effect that cloperastine is breathed heavily.Lumbricus also has good nutritive value, and according to surveying and determination, the protein content of Lumbricus accounts for the 53.5%-65.1% of dry weight, and fat content is about 4.4%-17.38%, and carbohydrate is about 11%-17.4%, moisture 7.8%-23%.Possibly together with abundant vitamin D (accounting for the 0.04%-0.073% of fresh body weight) in Lumbricus body, and the mineral element such as calcium and phosphorus (accounting for the 0.124%-0.188% of fresh body weight).Containing multiple medicinal ingredients such as ground LONGSU, Pheretima toxolysin, xanthine, antihistaminic and vitamin Bs in Lumbricus body.
Although Lumbricus is edible, but it is bad to there is outward appearance, affects appetite, and the fishy smell contained in Lumbricus can cause the deficiency that the food taste of preparation is the best.It addition, yet there are no the report utilizing the enzymolysis solution of Lumbricus and other materials to prepare beverage.
Summary of the invention
For problem above, it is an object of the invention to provide preparation method and the products obtained therefrom of a kind of earthworm protein beverage, the present invention uses Lumbricus enzymolysis new technology, and the fishy smell of earthworm enzymatic hydrolysate can be eliminated, preparation beverage not only clear, sour-sweet moderate, local flavor is natural, and nutritious, and there is health care.
Concrete technical scheme is as follows:
(1), the process of raw material: choosing Lumbricus is raw material, washes, with Lumbricus: water=1: 4-6 mass is compared to proportioning, soaks 2 hours, and rear feeding in tissue mashing machine is homogenized；
(2), enzymolysis: adding compound enzyme in Lumbricus is starched, in its compound enzyme, flavor protease and tryptic consumption are that every gram of Lumbricus slurry weight adds 1000-1500 enzyme unit (u) alive, in 50-55 DEG C, pH 7.8-8.2 carry out enzyme digestion reaction 2-4h；Then enzyme denaturing 10-20 minute at 90-95 DEG C, then under 3000 revs/min of rotating speeds, centrifugal 15-20 minute, collects supernatant；
(3), eliminating sargassum smell, de-bitter, desolventing technology: the earthworm enzymatic hydrolysate after enzyme denaturing is added the yeast powder of the 3-5% of its weight, stirs under 30 DEG C of temperature conditionss, stand 90-120 minute, carry out defishying；Earthworm enzymatic hydrolysate after eliminating sargassum smell is added the beta-schardinger dextrin-of the 0.5-1% of its weight, uniform stirring 5-10 minute under the conditions of 45-55 DEG C, carry out de-suffering reason；Earthworm enzymatic hydrolysate after de-hardship is added the activated carbon of the 3-5% of its weight, under conditions of 50-70 DEG C, carries out desolventing technology 30-60 minute；
(4), sucking filtration: the earthworm enzymatic hydrolysate after eliminating sargassum smell, de-hardship, decolouring is filtered in vacuum filtration equipment, obtains transparent, the Lumbricus enzymolysis protein liquid of free from extraneous odour；
(5), allotment: weigh the Lumbricus enzymolysis protein liquid after concentration and add sucrose, citric acid, mix homogeneously, pasteurization, obtain earthworm protein beverage.
In said method, in step (2), described compound enzyme is made up of flavor protease and trypsin.
In said method, in step (5), every 100ml Lumbricus enzymolysis protein liquid adds 5-12g sucrose, 0.1-0.5g citric acid.
The invention have the advantages that
(1) the inventive method is unique, and technological process is simple, and easy to operate, low cost, the present invention selects Lumbricus to be raw material, and by generating substantial amounts of free amino acid and biologically active polypeptide after enzymolysis, its nutritive value is greatly improved, and is easily quickly absorbed by human body.
(2) kind of existing beverage is enriched, a kind of earthworm protein beverage of Promethean invention.
(3) instant invention overcomes the fishy smell that Lumbricus is intrinsic, well improve the technical barrier of Lumbricus food taste difference.
(4) the preparation method rational technology of this earthworm protein beverage, makes simple, and strong operability is suitable for large-scale production.
Detailed description of the invention
Below by specific embodiment, the present invention will be further elaborated, it should be appreciated that, the description below is merely to explain the present invention, and is not limited thereof.
Embodiment
1
1, the process of raw material: choosing Lumbricus is raw material, washes, with Lumbricus: water=1: 4 mass are compared to proportioning, soaks 2 hours, and rear feeding in tissue mashing machine is homogenized.
2, enzymolysis: add compound enzyme in Lumbricus is starched, in its compound enzyme, flavor protease and tryptic consumption are that every gram of Lumbricus slurry weight adds 1000 enzymes and lives unit (u), in 55 DEG C, pH 8.2 carry out enzyme digestion reaction 2h；Then enzyme denaturing 20 minutes at 90 DEG C, then under 3000 revs/min of rotating speeds, centrifugal 15 minutes, collect supernatant；Wherein, described compound enzyme is made up of flavor protease and trypsin.
3, eliminating sargassum smell, de-bitter, desolventing technology: the earthworm enzymatic hydrolysate after enzyme denaturing is added its weight 5% yeast powder, stir under 30 DEG C of temperature conditionss, stand 120 minutes, carry out defishying；Earthworm enzymatic hydrolysate after eliminating sargassum smell is added its weight 0.5% beta-schardinger dextrin-, uniform stirring 5 minutes under the conditions of 45-55 DEG C, carry out de-suffering reason；Earthworm enzymatic hydrolysate after de-hardship is added its weight 3% activated carbon, under conditions of 50 DEG C, carry out desolventing technology 60 minutes.
4, sucking filtration: the earthworm enzymatic hydrolysate after eliminating sargassum smell, de-hardship, decolouring is filtered in vacuum filtration equipment, obtains transparent, the Lumbricus enzymolysis protein liquid of free from extraneous odour.
5, allotment: weigh the Lumbricus enzymolysis protein liquid after concentration and add sucrose, citric acid, mix homogeneously, pasteurization, obtain earthworm protein beverage；Wherein, every 100ml Lumbricus enzymolysis protein liquid adds 5g sucrose, 0.1g citric acid.
Embodiment
2
1, the process of raw material: choosing Lumbricus is raw material, washes, with Lumbricus: water=1: 6 mass are compared to proportioning, soaks 2 hours, and rear feeding in tissue mashing machine is homogenized.
2, enzymolysis: add compound enzyme in Lumbricus is starched, in its compound enzyme, flavor protease and tryptic consumption are that every gram of Lumbricus slurry weight adds 1500 enzymes and lives unit (u), in 50 DEG C, pH 7.8 carry out enzyme digestion reaction 4h；Then enzyme denaturing 10 minutes at 95 DEG C, then under 3000 revs/min of rotating speeds, centrifugal 20 minutes, collect supernatant；Wherein, described compound enzyme is made up of flavor protease and trypsin.
3, eliminating sargassum smell, de-bitter, desolventing technology: the earthworm enzymatic hydrolysate after enzyme denaturing is added its weight 3% yeast powder, stir under 30 DEG C of temperature conditionss, stand 90 minutes, carry out defishying；Earthworm enzymatic hydrolysate after eliminating sargassum smell is added its weight 1% beta-schardinger dextrin-, uniform stirring 10 minutes under the conditions of 45-55 DEG C, carry out de-suffering reason；Earthworm enzymatic hydrolysate after de-hardship is added its weight 5% activated carbon, under conditions of 70 DEG C, carry out desolventing technology 30 minutes.
4, sucking filtration: the earthworm enzymatic hydrolysate after eliminating sargassum smell, de-hardship, decolouring is filtered in vacuum filtration equipment, obtains transparent, the Lumbricus enzymolysis protein liquid of free from extraneous odour.
5, allotment: weigh the Lumbricus enzymolysis protein liquid after concentration and add sucrose, citric acid, mix homogeneously, pasteurization, obtain earthworm protein beverage；Wherein, every 100ml Lumbricus enzymolysis protein liquid adds 12g sucrose, 0.5g citric acid.
Below the mouthfeel of beverage of the present invention is evaluated.
Random invitation 20 people, the maleest 10 people, female 10 people, minimal ages 5 years old, max age 50 years old, 34.7 years old mean age.These 20 experimenters are invited to taste the beverage of the present invention, full marks 100 points respectively.Experimenter is divided into 2 groups, and one of which tastes the beverage of embodiment 1, and another group tastes the beverage of embodiment 2, evaluation result such as following table:
Result is judged by table 1 consumer
After above-mentioned evaluation completes, all experimenters all represent that the beverage exquisiteness of the present invention is lubricious, tasty and refreshing, sour-sweet, soft, illustrate that the beverage of the present invention has the highest traction.
Claims (3)
1. the preparation method of earthworm protein beverage and a products obtained therefrom, is characterized in that comprising the following steps:
(1), the process of raw material: choosing Lumbricus is raw material, washes, with Lumbricus: water=1: 4-6 mass is compared to proportioning, soaks 2 hours, and rear feeding in tissue mashing machine is homogenized；
(2), enzymolysis: adding compound enzyme in Lumbricus is starched, in its compound enzyme, flavor protease and tryptic consumption are that every gram of Lumbricus slurry weight adds 1000-1500 enzyme unit (u) alive, in 50-55 DEG C, pH 7.8-8.2 carry out enzyme digestion reaction 2-4h；Then enzyme denaturing 10-20 minute at 90-95 DEG C, then under 3000 revs/min of rotating speeds, centrifugal 15-20 minute, collects supernatant；
(3), eliminating sargassum smell, de-bitter, desolventing technology: the earthworm enzymatic hydrolysate after enzyme denaturing is added the yeast powder of the 3-5% of its weight, stirs under 30 DEG C of temperature conditionss, stand 90-120 minute, carry out defishying；Earthworm enzymatic hydrolysate after eliminating sargassum smell is added the beta-schardinger dextrin-of the 0.5-1% of its weight, uniform stirring 5-10 minute under the conditions of 45-55 DEG C, carry out de-suffering reason；Earthworm enzymatic hydrolysate after de-hardship is added the activated carbon of the 3-5% of its weight, under conditions of 50-70 DEG C, carries out desolventing technology 30-60 minute；
(4), sucking filtration: the earthworm enzymatic hydrolysate after eliminating sargassum smell, de-hardship, decolouring is filtered in vacuum filtration equipment, obtains transparent, the Lumbricus enzymolysis protein liquid of free from extraneous odour；
(5), allotment: weigh the Lumbricus enzymolysis protein liquid after concentration and add sucrose, citric acid, mix homogeneously, pasteurization, obtain earthworm protein beverage.
Method the most according to claim 1, is characterized in that: in step (2), described compound enzyme is made up of flavor protease and trypsin.
Method the most according to claim 1, is characterized in that: in step (5), adds 5-12g sucrose, 0.1-0.5g citric acid in every 100ml Lumbricus enzymolysis protein liquid.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107495214A (en) * | 2017-09-18 | 2017-12-22 | 江苏步龙生物科技有限公司 | A kind of compound earthworm powder and preparation method thereof |
| CN110477400A (en) * | 2017-12-26 | 2019-11-22 | 兰溪市沉默生物科技有限公司 | A kind of preparation method of the polypeptide compound of pheretima preparation |
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| CN110477400A (en) * | 2017-12-26 | 2019-11-22 | 兰溪市沉默生物科技有限公司 | A kind of preparation method of the polypeptide compound of pheretima preparation |
| CN114982880A (en) * | 2022-05-18 | 2022-09-02 | 阳江职业技术学院 | Functional protein beverage of gramineous insect and preparation method thereof |
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