CN103479704A - Method for extracting total flavones from chrysanthemum - Google Patents
Method for extracting total flavones from chrysanthemum Download PDFInfo
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- CN103479704A CN103479704A CN201310451031.4A CN201310451031A CN103479704A CN 103479704 A CN103479704 A CN 103479704A CN 201310451031 A CN201310451031 A CN 201310451031A CN 103479704 A CN103479704 A CN 103479704A
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Abstract
The invention discloses a method for extracting total flavones from chrysanthemum, and belongs to the technical field of extraction of effective components of plants. The method comprises steps as follows: after the chrysanthemum is smashed and sieved by a 20-mesh sieve, petroleum ether is added, the mixture is uniformly mixed and filtered by filter paper after sufficiently decolored, and filtered slag is taken and dried for standby application; and pretreated materials are accurately weighed, 40%-70% of an ethanol solution is added in a ratio (g/mL) of the materials to the solution of 1: (10-30), the mixture is sufficiently and uniformly shaken and immersed for one hour, and extraction is performed at the temperature ranging from 20 DEG C to 50 DEG C with the ultrasonic frequency of 40 KHz for 20-35 minutes to obtain an extract of total flavones of chrysanthemum. The extract is used for preparing anti-inflammation drugs.
Description
Technical field
The present invention relates to the extracting method technical field of effective ingredients in plant, in particular a kind of method of supersound extraction chrysanthemum total flavone.
Background technology
Flos Chrysanthemi, another name is named again Hemerocallis citrina Baroni, longevity visitor, Jin Ying, Huang Hua, Qiu Ju, Tao Ju, skill chrysanthemum.Perennial catananche, its petal is ligule or tubular.Flos Chrysanthemi in China plantation extensively, is drunk with a long historyly, is the medicine food excellent representative plant of holding concurrently.China's Flos Chrysanthemi mainly contains 8 kinds, is respectively Flos Chrysanthemi, milli chrysanthemum, Flos Chrysanthemi, Flos Chrysanthemi, Flos Chrysanthemi, Flos Chrysanthemi, Flos Chrysanthemi, Herba Tagetis Patulae.Chrysanthemum morifolium in China's Chinese medicine is the dry capitulum of feverfew Flos Chrysanthemi, has the effect of dispelling wind improving eyesight, heat-clearing and toxic substances removing, the cool gallbladder of suppressing the hyperactive liver, the diseases such as primary treatment headache, dizzy, conjunctival congestion, irritable feverish sensation of chest and heart, furuncle, toxic swelling.Modern pharmacological research shows, the main effective ingredient of Flos Chrysanthemi is flavone compound, triterpenoid compound and volatilization wet goods, has treatment coronary heart disease, reduces blood pressure, prevents hyperlipidemia, antibiotic, antiviral, antiinflammatory, the anti-ageing multiple pharmacologically active of waiting for a long time.
Total flavones is the main pharmacodynamics active component of Flos Chrysanthemi, and cardiovascular system is had good action, has the multiple physiologically actives such as effect that suppress cholinesterase, often with this, estimates the quality of Flos Chrysanthemi.
Flavonoids has multiple biological activity, and the functions such as antiulcer, antiallergic, antibiotic, antiinflammatory, antioxidation, defying age, blood fat reducing, treatment cardiovascular and cerebrovascular disease are arranged.Ultrasound wave assisted extraction natural product composition has short, noiselessness of operating time, pollution-free, energy consumption is low, the extraction ratio high, belongs to eco-friendly extraction method.
The present invention's application orthogonal design as solid-liquid ratio, concentration of alcohol, extraction temperature, extraction time are studied, draws best ultrasonic extracting method to main affecting factors.
Technical scheme of the present invention is as follows:
1, a kind of method of supersound extraction chrysanthemum total flavone, is characterized in that, after the Flos Chrysanthemi herb is pulverized, crosses 20 mesh sieves, adds the petroleum ether mix homogeneously, and fully decolouring, by the filter paper sucking filtration, is got filtering residue and volatilized standby; Accurately take the material that pretreatment is good, by solid-liquid ratio (g/mL) 1: 10-30 adds the alcoholic solution of 40-70%, fully shakes up rear immersion 1 hour, under temperature 20-50 ℃, supersonic frequency 40KHz extracts 20-35min, obtains the chrysanthemum total flavone extract, is applied to prepare anti-inflammatory drug.
2, method according to claim 1, is characterized in that, solid-liquid ratio (g/mL) 1: 16, and alcoholic solution concentration 70%, extract 20 ℃ of temperature, extraction time 20min.
3, method according to claim 1, is characterized in that, after the Flos Chrysanthemi herb is pulverized, crosses 20 mesh sieves, by solid-liquid ratio (g/mL), adds the petroleum ether mix homogeneously at 1: 10, decolours twice.
The specific embodiment
Embodiment 1
The Flos Chrysanthemi herb is crossed 20 mesh sieves after pulverizing, and by solid-liquid ratio (g/mL), adds the petroleum ether mix homogeneously at 1: 8, fully after decolouring, through the filter paper sucking filtration, filtering residue adds solid-liquid ratio (g/mL) to add the petroleum ether mix homogeneously at 1: 8 again, fully after decolouring, through the filter paper sucking filtration, get filtering residue and volatilize standby; Accurately take the material that pretreatment is good, add 70% alcoholic solution at 1: 15 by solid-liquid ratio (g/mL), fully shake up rear immersion 1 hour, 20 ℃ of temperature, extract 30min under supersonic frequency 40KHz, the chrysanthemum total flavone extract, through the concentration of spectrophotometry chrysanthemum total flavone, be 24.7%.
Embodiment 2
The Flos Chrysanthemi herb is crossed 20 mesh sieves after pulverizing, and by solid-liquid ratio (g/mL), adds the petroleum ether mix homogeneously at 1: 10, fully after decolouring, through the filter paper sucking filtration, filtering residue adds solid-liquid ratio (g/mL) to add the petroleum ether mix homogeneously at 1: 6 again, fully after decolouring, through the filter paper sucking filtration, get filtering residue and volatilize standby; Accurately take the material that pretreatment is good, add 50% alcoholic solution at 1: 20 by solid-liquid ratio (g/mL), fully shake up rear immersion 1 hour, 40 ℃ of temperature, extract 30min under supersonic frequency 40KHz, the chrysanthemum total flavone extract, through the concentration of spectrophotometry chrysanthemum total flavone, be 21.5%.
Embodiment 3
The Flos Chrysanthemi herb is crossed 20 mesh sieves after pulverizing, and by solid-liquid ratio (g/mL), adds the petroleum ether mix homogeneously at 1: 10, fully after decolouring, through the filter paper sucking filtration, filtering residue adds solid-liquid ratio (g/mL) to add the petroleum ether mix homogeneously at 1: 10 again, fully after decolouring, through the filter paper sucking filtration, get filtering residue and volatilize standby; Accurately take the material that pretreatment is good, add 60% alcoholic solution at 1: 15 by solid-liquid ratio (g/mL), fully shake up rear immersion 1 hour, 20 ℃ of temperature, extract 30min under supersonic frequency 40KHz, the chrysanthemum total flavone extract, through the concentration of spectrophotometry chrysanthemum total flavone, be 23.6%.
Claims (3)
1. the method for a supersound extraction chrysanthemum total flavone, is characterized in that, after the Flos Chrysanthemi herb is pulverized, crosses 20 mesh sieves, adds the petroleum ether mix homogeneously, and fully decolouring, by the filter paper sucking filtration, is got filtering residue and volatilized standby; Accurately take the material that pretreatment is good, by solid-liquid ratio (g/mL) 1: 10-30 adds the alcoholic solution of 40-70%, fully shakes up rear immersion 1 hour, under temperature 20-50 ℃, supersonic frequency 40KHz extracts 20-35min, obtains the chrysanthemum total flavone extract, is applied to prepare anti-inflammatory drug.
2. method according to claim 1, is characterized in that, solid-liquid ratio (g/mL) 1: 16, and alcoholic solution concentration 70%, extract 20 ℃ of temperature, extraction time 20min.
3. method according to claim 1, is characterized in that, after the Flos Chrysanthemi herb is pulverized, crosses 20 mesh sieves, by solid-liquid ratio (g/mL), adds the petroleum ether mix homogeneously at 1: 10, decolours twice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310451031.4A CN103479704A (en) | 2013-09-27 | 2013-09-27 | Method for extracting total flavones from chrysanthemum |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310451031.4A CN103479704A (en) | 2013-09-27 | 2013-09-27 | Method for extracting total flavones from chrysanthemum |
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| CN103479704A true CN103479704A (en) | 2014-01-01 |
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| CN201310451031.4A Pending CN103479704A (en) | 2013-09-27 | 2013-09-27 | Method for extracting total flavones from chrysanthemum |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104939309A (en) * | 2015-06-17 | 2015-09-30 | 云南中烟工业有限责任公司 | Florists chrysanthemum glycerol triacetate extract and application thereof in cigarettes |
| CN105030869A (en) * | 2015-06-29 | 2015-11-11 | 安徽瑞思威尔科技有限公司 | Bo-chrysanthemum total flavone extract preparation method based on response surface optimization |
-
2013
- 2013-09-27 CN CN201310451031.4A patent/CN103479704A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104939309A (en) * | 2015-06-17 | 2015-09-30 | 云南中烟工业有限责任公司 | Florists chrysanthemum glycerol triacetate extract and application thereof in cigarettes |
| CN105030869A (en) * | 2015-06-29 | 2015-11-11 | 安徽瑞思威尔科技有限公司 | Bo-chrysanthemum total flavone extract preparation method based on response surface optimization |
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Application publication date: 20140101 |