CN103478402B - Method for extracting pea molasses and pea protein isolate from pea starch production wastewater - Google Patents
Method for extracting pea molasses and pea protein isolate from pea starch production wastewater Download PDFInfo
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- CN103478402B CN103478402B CN201310480123.5A CN201310480123A CN103478402B CN 103478402 B CN103478402 B CN 103478402B CN 201310480123 A CN201310480123 A CN 201310480123A CN 103478402 B CN103478402 B CN 103478402B
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Abstract
The invention discloses a method for extracting pea molasses and pea protein isolate from pea starch production wastewater. The method comprises the steps in sequence of protein extraction, acid sedimentation separation, washing separation, neutralization, whey concentration, flash distillation for sterilization, drying and powder-sieving. The extraction method can improve the purity and yield of pea protein isolate efficiently; wastewater generated from the production of pea protein isolate is collected for extracting pea molasses for use, so that the wastewater is fully utilized, and resource waste and environment pollution are alleviated. The pea protein isolate produced by the method has the content of more than 90%, has quite high water-retaining property and oil absorption capacity, and has gel formability better than that of soybean protein isolate. The pea molasses produced by the method is a brown syrup-shaped substance with the content of total sugar within 50%-60%, and has relatively great application prospect in the aspects of foods, medicines and light industry.
Description
Technical field
The present invention is specifically related to a kind of method extracting pea molasses and pea separation protein from the waste water that processing pea starch process produces.
Background technology
At present, the processing majority of domestic enterprise to pea rests on the stage of producing starch, making bean vermicelli.Produce containing a large amount of nutriments such as protein, dietary fiber, compound sugar in the waste water of pea starch process generation, if directly discharge meeting serious waste resource, contaminated environment.In recent years, the research of pea separation protein extracting method is increasing, from the waste water producing starch, mainly take the heavy method of the molten acid of alkali to extract pea protein in prior art, its following shortcoming existed: 1, existing extraction process makes the purity of the pea separation protein produced generally below 85%, product purity is lower, the functional impact being subject to purity; 2, the extracting method of existing pea separation protein is subject to the restriction of technological parameter and appointed condition, and protein yield is relatively low; 3, the waste water after extraction and isolation albumen is not fully used, and directly discharges, and causes the serious waste of resource.
Meanwhile, soy molasses produces the accessory substance in FSPC (being called for short ALSPC) process as alcohol method, and its functional achievement in research is reported out successively.And in pea separation protein process, a kind of molasses being rich in plurality of active ingredients can be produced equally---pea molasses.Pea molasses components is comparatively complicated, it is the aggregate of various plants compound, containing abundant free sugar, vitamin and mineral matter, can be used as the extraction raw material of pea compound sugar, cellulose etc., also can be used as nutritive value and palatability that feed addictive improves feed, there is important biological action and higher economic worth.
Therefore, a kind of novel extraction process of exploitation is needed badly in prior art, the waste water produced in pea starch production process is fully utilized, while production high purity separation albumen, extract the pea molasses that quality is good, be of high nutritive value, create higher value of the product, reduce the wasting of resources, reduce environmental pollution simultaneously, there is very high environmental benefit, economic benefit and social benefit.
Summary of the invention
The object of the invention is to change the wasting of resources phenomenon occurred in current pea process, a kind of method extracting pea molasses and pea separation protein in pea starch factory effluent is provided, the method can produce high-quality pea separation protein and pea molasses, improves production capacity and the added value of product.
The present invention is achieved by the following technical solutions: a kind of method extracting pea molasses and pea separation protein in pea starch factory effluent, comprises the following steps:
1) protein extraction
Farinaceous size is placed in premixing tank, while stirring the alkali lye prepared is poured in premixing tank uniformly, regulate plasm PH value to 11.0-12.4, Extracting temperature controls at 30-40 DEG C, extraction time 30-60min, albumen is fully dissolved, and then centrifugal by carrying out in mixed liquor transport pump to horizontal screw centrifuge, the proteinaceous solution of isolated richness injects settling tank;
2) acid is heavy is separated
The protein solution injecting settling tank is slowly added watery hydrochloric acid while stirring, regulate PH to 4.0-4.5, protein is separated out when its isoelectric point, sedimentation time 20-30min, precipitated liquid stack centrifuge is separated, obtain protein-contg primary curd and whey, whey pumped in whey neutralizing tank, primary curd pumps in secondary tank;
3) washing is separated
Rinse the albumen curd in secondary tank with the clear water of 47-53 DEG C, ratio of water to material is (1.5-2): 1, and the mixed serum lamination seperator after washing is separated, and pumps in albumen neutralizing tank by being separated the secondary albumen curd obtained;
4) neutralize
Sig water is injected respectively whey neutralizing tank and albumen neutralizing tank, the pH value of whey is adjusted to 6.8-7.5, the pH value of secondary curdled milk is adjusted to 6.5-7.0;
5) whey concentrates
Whey after neutralization is injected vacuum concentration equipment and is concentrated, and make its water content drop to 45%-55%, concentrate homogenizer carries out homogeneous, namely defines tan syrupy shape material---pea molasses;
6) flash distillation sterilization
Albumen curd after neutralization pumps in flash tank after high-pressure homogeneous, flash distillation sterilization, and sterilization temperature controls at 110-120 DEG C, and sterilizing time is 15-20s;
7) dry sieve powder
Concentrate after flash distillation sterilization is evenly sent in drying tower through high-pressure pump and is carried out spraying dry, after dried albumen powder sieves, sends into warehouse for finished product and packs.
The invention has the beneficial effects as follows: extracting method of the present invention effectively can improve purity and the yield of pea separation protein, the waste water produced after pea separation protein is collected simultaneously, extract pea molasses to be used, waste water after production protein isolate is fully used, reduce the wasting of resources, reduce environmental pollution.
The content of the pea separation protein that the present invention produces is more than 90%, and have quite high water-retaining property and oil absorption, gel forming is better than soybean protein isolate, can be used for the additive of the numerous food such as meat products, Flour product, dairy products, functional beverage.The pea molasses produced are a kind of tan syrupy shape materials, total sugar content is at 50%-60%, can be used as energy source and nutrient is added in the feed of ruminant and bird, reduce Feed Manufacturing cost, improve feed palatability, increase animal feed intake, also directly can be used as food additives, also can be used as the extraction raw material of pea dietary fiber, pea compound sugar etc., in food, medicine, light industry, all have larger application prospect.The production technology of pea separation protein and pea molasses is joined together by the present invention, has advantage that is highly efficient and productive, energy-saving and environmental protection.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
Farinaceous size is placed in premixing tank, is poured into uniformly in premixing tank by the sig water prepared while stirring, regulate plasm PH value to 11.0, Extracting temperature controls at 35 DEG C, and extraction time 30min makes albumen fully dissolve.Then centrifugal by carrying out in mixed liquor transport pump to horizontal screw centrifuge, the proteinaceous solution of isolated richness injects settling tank, slowly adds watery hydrochloric acid while stirring, regulates PH to 4.0, protein is separated out, sedimentation time 30min.Precipitated liquid stack centrifuge is separated, and obtains whey and protein-contg primary curd.Whey is pumped in whey neutralizing tank, slowly add sig water while stirring, regulate PH to 6.8, whey after neutralization is injected vacuum concentration equipment and is concentrated, make its water content drop to 45%, concentrate homogenizer carries out homogeneous, namely defines tan syrupy shape material---pea molasses.Albumen primary curd is pumped in secondary tank, the albumen curd in secondary tank is rinsed with the clear water of 47-53 DEG C, ratio of water to material is 1.5:1, mixed serum lamination seperator after washing is separated, pump in albumen neutralizing tank by being separated the secondary albumen curd obtained, slowly add sig water while stirring, make its pH value be adjusted to 6.8.Albumen curd after neutralization pumps in flash tank after high-pressure homogeneous, flash distillation sterilization, sterilization temperature 110 DEG C, and sterilizing time is 15s.Concentrate after sterilization is evenly sent in drying tower through high-pressure pump and is carried out spraying dry, after dried albumen powder sieves, sends into warehouse for finished product and packs.
Embodiment 2
Farinaceous size is placed in premixing tank, is poured into uniformly in premixing tank by the sig water prepared while stirring, regulate plasm PH value to 11.5, Extracting temperature controls at 30 DEG C, and extraction time 60min makes albumen fully dissolve.Then centrifugal by carrying out in mixed liquor transport pump to horizontal screw centrifuge, the proteinaceous solution of isolated richness injects settling tank, slowly adds watery hydrochloric acid while stirring, regulates PH to 4.0, protein is separated out, sedimentation time 20min.Precipitated liquid stack centrifuge is separated, and obtains whey and protein-contg primary curd.Whey is pumped in whey neutralizing tank, slowly add sig water while stirring, regulate PH to 7.2, whey after neutralization is injected vacuum concentration equipment and is concentrated, make its water content drop to 47%, concentrate homogenizer carries out homogeneous, namely defines tan syrupy shape material---pea molasses.Albumen primary curd is pumped in secondary tank, the albumen curd in secondary tank is rinsed with the clear water of 47-53 DEG C, ratio of water to material is 1.5:1, mixed serum lamination seperator after washing is separated, pump in albumen neutralizing tank by being separated the secondary albumen curd obtained, slowly add sig water while stirring, make its pH value be adjusted to 7.0.Albumen curd after neutralization pumps in flash tank after high-pressure homogeneous, flash distillation sterilization, sterilization temperature 110 DEG C, and sterilizing time is 20s.Concentrate after sterilization is evenly sent in drying tower through high-pressure pump and is carried out spraying dry, after dried albumen powder sieves, sends into warehouse for finished product and packs.
Embodiment 3
Farinaceous size is placed in premixing tank, is poured into uniformly in premixing tank by the sig water prepared while stirring, regulate plasm PH value to 12.4, Extracting temperature controls at 40 DEG C, and extraction time 40min makes albumen fully dissolve.Then centrifugal by carrying out in mixed liquor transport pump to horizontal screw centrifuge, the proteinaceous solution of isolated richness injects settling tank, slowly adds watery hydrochloric acid while stirring, regulates PH to 4.5, protein is separated out, sedimentation time 30min.Precipitated liquid stack centrifuge is separated, and obtains whey and protein-contg primary curd.Whey is pumped in whey neutralizing tank, slowly add sig water while stirring, regulate PH to 7.5, whey after neutralization is injected vacuum concentration equipment and is concentrated, make its water content drop to 55%, concentrate homogenizer carries out homogeneous, namely defines tan syrupy shape material---pea molasses.Albumen primary curd is pumped in secondary tank, the albumen curd in secondary tank is rinsed with the clear water of 47-53 DEG C, ratio of water to material is 2:1, mixed serum lamination seperator after washing is separated, pump in albumen neutralizing tank by being separated the secondary albumen curd obtained, slowly add sig water while stirring, make its pH value be adjusted to 6.5.Albumen curd after neutralization pumps in flash tank after high-pressure homogeneous, flash distillation sterilization, sterilization temperature 120 DEG C, and sterilizing time is 15s.Concentrate after sterilization is evenly sent in drying tower through high-pressure pump and is carried out spraying dry, after dried albumen powder sieves, sends into warehouse for finished product and packs.
Test the contrast of 1 pea protein physical and chemical index
The pea separation protein that Example 1,2,3 extracts, the pea separation protein 1 namely in following table, pea separation protein 2 and pea separation protein 3, carry out physical and chemical composition mensuration, simultaneously, buy the pea separation protein on market and soybean protein isolate, measure its physical and chemical composition, result is as shown in table 1:
Table 1 pea separation protein physical and chemical composition detects table
As shown in Table 1, the pea separation protein purity that the method is produced is higher, and the high-purity soybean protein isolate purity that market is sold is suitable, has very high nutritive value.
Test the functional contrast of 2 pea protein
The pea separation protein that Example 1,2,3 extracts, namely the pea separation protein 1 in following table, pea separation protein 2 and pea separation protein 3, carry out the functional assays of being correlated with, with the pea separation protein that market is bought and soybean protein isolate related check Data Comparison, result is as shown in table 2:
The functional detection tables of data of table 2 pea separation protein
As shown in Table 2, the pea separation protein of this explained hereafter is water-soluble higher, and in foaming characteristic, water suction oil absorption, emulsibility and emulsion stability aspect are all better than soybean protein isolate.
Test 3 pea molasses physical and chemical indexs to detect
The pea molasses that Example 1,2,3 extracts, carry out the mensuration of the component content of being correlated with, contrast with the corresponding data of soy molasses, result is as shown in table 3:
Table 3 pea molasses detect tables of data
As shown in Table 3, in the pea molasses that method of the present invention is extracted, total sugar content is higher, and the composition of soy molasses is suitable, has good market application foreground.
Finally should be noted that; above content is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; the simple modification that those of ordinary skill in the art carries out technical scheme of the present invention or equivalently to replace, does not all depart from essence and the scope of technical solution of the present invention.
Claims (1)
1. extract a method for pea molasses and pea separation protein in pea starch factory effluent, it is characterized in that comprising the following steps:
1) protein extraction
Farinaceous size is placed in premixing tank, while stirring the alkali lye prepared is poured in premixing tank uniformly, regulate plasm PH value to 11.0-12.4, Extracting temperature controls at 30-40 DEG C, extraction time 30-60min, albumen is fully dissolved, and then centrifugal by carrying out in mixed liquor transport pump to horizontal screw centrifuge, the proteinaceous solution of isolated richness injects settling tank;
2) acid is heavy is separated
The protein solution injecting settling tank is slowly added watery hydrochloric acid while stirring, regulate PH to 4.0-4.5, protein is separated out when its isoelectric point, sedimentation time 20-30min, precipitated liquid stack centrifuge is separated, obtain protein-contg primary curd and whey, whey pumped in whey neutralizing tank, primary curd pumps in secondary tank;
3) washing is separated
Rinse the albumen curd in secondary tank with the clear water of 47-53 DEG C, ratio of water to material is (1.5-2): 1, and the mixed serum lamination seperator after washing is separated, and pumps in albumen neutralizing tank by being separated the secondary albumen curd obtained;
4) neutralize
Sig water is injected respectively whey neutralizing tank and albumen neutralizing tank, the pH value of whey is adjusted to 6.8-7.5, the pH value of secondary curdled milk is adjusted to 6.5-7.0;
5) whey concentrates
Whey after neutralization is injected vacuum concentration equipment and is concentrated, and make its water content drop to 45%-55%, concentrate homogenizer carries out homogeneous, namely defines tan syrupy shape material---pea molasses;
6) flash distillation sterilization
Albumen curd after neutralization pumps in flash tank after high-pressure homogeneous, flash distillation sterilization, and sterilization temperature controls at 110-120 DEG C, and sterilizing time is 15-20s;
7) dry sieve powder
Concentrate after flash distillation sterilization is evenly sent in drying tower through high-pressure pump and is carried out spraying dry, after dried albumen powder sieves, sends into warehouse for finished product and packs.
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| CN106490626A (en) * | 2015-09-06 | 2017-03-15 | 青海合杰生物制品有限责任公司 | A kind of nutrient substance comprehensively extracting and purifying method in beans |
| CN105454622A (en) * | 2015-11-16 | 2016-04-06 | 宿州市皖神面制品有限公司 | Production method of pea protein powder |
| CN106867937A (en) * | 2017-03-11 | 2017-06-20 | 鲁东大学 | With the bacterial strain of pea protein wastewater liquid fermenting and producing Bacillus subtilis natto microbial inoculum, method and application |
| CN107307190A (en) * | 2017-08-11 | 2017-11-03 | 临邑禹王植物蛋白有限公司 | A kind of technique of the aquatic production feed oligosaccharide of soy-bean whey |
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| CN109907156A (en) * | 2019-03-19 | 2019-06-21 | 烟台东方蛋白科技有限公司 | A kind of production method reconstituting type pea separation protein finished product |
| CN110432375A (en) * | 2019-08-28 | 2019-11-12 | 宿州市皖神面制品有限公司 | A kind of process for extracting pea separation protein |
| CN110547356A (en) * | 2019-09-23 | 2019-12-10 | 张立峰 | Functional bean whey protein and production method and application thereof |
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