CN103461388A - Microbial herbicide and preparation method thereof - Google Patents
Microbial herbicide and preparation method thereof Download PDFInfo
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- CN103461388A CN103461388A CN2013104072860A CN201310407286A CN103461388A CN 103461388 A CN103461388 A CN 103461388A CN 2013104072860 A CN2013104072860 A CN 2013104072860A CN 201310407286 A CN201310407286 A CN 201310407286A CN 103461388 A CN103461388 A CN 103461388A
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- spore suspension
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Abstract
The invention relates to a microbial herbicide and a preparation method thereof, belonging to the technical field of pesticide production. The microbial herbicide is prepared from the following ingredients in parts by weight: 10-35 parts of spore suspension mother liquor of Alternaria augustiovoidea strains AAEC05-3, 15-25 parts of spore suspension mother liquor of A. amaranthi-3 and 5-12 parts of Tween-80. The preparation method comprises the steps of mixing the two kinds of spore suspension mother liquor according to a certain ratio, then, adding Tween at the spray volume of 100-150mL/m<2>, and carrying out moisture retention for 40-60 hours, thereby obtaining an aqueous microbial herbicide. The herbicide can effectively inhibit Echinochloa crusgalli and Amaranthus retroflexus, is non-toxic and readily degradable, and is a product with development and application prospects.
Description
Technical field
The present invention relates to a kind of microbial herbicide formula and preparation method thereof, belong to the pesticide processing technical field.
Background technology
Weeds are considered to one of biological epidemics that have a strong impact on Agriculture and Environment always.Hundred million workdays of the about 1500-3000 of the annual farmland weeding recruitment in the whole world, but crop smothering still causes underproduction 10%-25%, and wherein the cereal underproduction is over 1.5 hundred million tons.After nineteen forty-two invention 2,4-D, risen chemical herbicide industry.Not weeding is compared, and chemical herbicide has been retrieved approximately 50% grain yield loss.But a large amount of uses of chemical herbicide have brought a series of ecological environment problem.As weed killer herbicide droplet drift, selectivity is not strong, and the half life period in environment is long and to the murder by poisoning of people, animal etc.Especially the appearance of antiweed weeds, more make development and the cost of exploitation chemical herbicide greatly increase, so the new approach of the continuous searching of researcher carrys out management of weeds.. find that after deliberation some microorganisms (pathogenic or non-plant pathogenetic bacteria, fungi) and secondary metabolite (phytotoxin) thereof have the potential of preventing and kill off or control weeds.The weed killer herbicide formed by these organisms and natural products exploitation is called Mirobial source herbicide.
The microbial control of weeds refers to and utilizes the comparatively single-minded plant pathogenic microorganisms of host range or its metabolite, will affect the ruderal species group control of economical activities of mankind below Damage threshold.Therefore, the purpose of microbial control weeds does not pull up weeds, but, according to the principle of synecology, many degree of a kind of weeds is reduced to economically or aesthetic admissible level.With chemical weed control, manually reach weeding by machine and compare, the advantage such as the microorganism weeding has small investment, economic benefit is high, the term of validity is long, pollution-free also can solve the pesticide resistance problem of weeds simultaneously.
Barnyard grass (
echinochloa crusgalli) and Amaranthus retroflexus (
amaranthus retroflexus) be universal malignant weed, the harm multiple kinds of crops.Because chemical herbicide is used not only contaminated environment for a long time in a large number, also cause the pesticide resistance problem of weeds to become increasingly conspicuous.Therefore, developing a kind of weed killer herbicide tool of preventing and kill off barnyard grass and Amaranthus retroflexus is of great significance.
Patent 201110199619.6 is openly invented a kind of weed killer herbicide, this herbicides for use in paddy, mix and make by nitrophenol, chlorophenol, carbamic acid, p-phenylenediamine (PPD), belong to chemical field, but this weed killer herbicide easily resolves into toxic compounds at occurring in nature, large to human body and environmental pressure, be difficult for degraded.Patent 200880025571.3 is openly invented a kind of new herbicides, pyrans diketone, sulfo-pyrans diketone and cyclohexanetriones compound, consists of, and this weed killer herbicide and Environmental compatibility are poor, is difficult for degraded, easily gives environment.At present, aspect the weed killer herbicide patent, major part is all chemical herbicide, about the patent of microbial herbicide, has not yet to see application, the present invention adopt barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) spore suspension mother liquor prepares a kind of microbial herbicide, this weed killer herbicide can not only avoid patent 201110199619.6 and 200880025571.3 two kinds of chemical herbicides of patent to be difficult for degraded, to shortcomings such as environment build-ups of pressure, also easily at occurring in nature, resolve into non-toxic compound, there is specificity, to non-target organism safety such as people, animal, natural enemies, and easily storage, easy to use, be a kind of green, efficient weed killer herbicide.
Summary of the invention
Purpose of the present invention provides a kind of microbial herbicide formula and preparation method thereof, this weed killer herbicide is a kind ofly easily at occurring in nature, to resolve into non-toxic compound, has specificity, to non-target organism safety such as people, animal, natural enemies, and easily storage, green easy to use, highy potent herbicide.
Purpose of the present invention is achieved through the following technical solutions:
A kind of microbial herbicide, each component and weight portion thereof are:
Barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) strains A AEC05-3 spore suspension mother liquor 10-35 part and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) spore suspension mother liquor 15-25 part, Tween 80 5-12 part.
A kind of preparation method of microbial herbicide:
Get barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) strains A AEC05-3 spore suspension mother liquor 10-35 part and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) spore suspension mother liquor 15-25 part, after two kinds of spore suspension mother liquors are mixed by a certain percentage, adding tween, spouting liquid is 100-150mL/m
2, moisturizing 40-60h, obtain microbial herbicide.
Beneficial effect of the present invention:
The present invention is a kind ofly easily resolved into non-toxic compound at occurring in nature, has specificity, to non-target organism safety such as people, animal, natural enemies, and easily storage, green easy to use, highy potent herbicide.
Embodiment
Below by embodiment, the present invention is described in further details, these embodiment only are used for illustrating the present invention, do not limit the scope of the invention.
Embodiment 1
A kind of microbial herbicide is comprised of following each component and weight portion thereof:
Barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 10 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 25 parts, spore suspension mother liquor, 7 parts of Tween 80s.
The preparation method:
Get barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 10 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 25 parts, spore suspension mother liquor, after two kinds of spore suspension mother liquors are mixed, adding tween, spouting liquid is 100mL/m
2, moisturizing 54h, obtain the microorganism Weeding water agent.
Embodiment 2
A kind of microbial herbicide is comprised of following each component and weight portion thereof:
Barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 18 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 15 parts, spore suspension mother liquor, 10 parts of Tween 80s.
The preparation method:
Get barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 18 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 15 parts, spore suspension mother liquor, after two kinds of spore suspension mother liquors are mixed, adding tween, spouting liquid is 120mL/m
2, moisturizing 45h, obtain the microorganism Weeding water agent.
Embodiment 3
A kind of microbial herbicide is comprised of following each component and weight portion thereof:
Barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 30 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 20 parts, spore suspension mother liquor, 10 parts of Tween 80s.
The preparation method:
Get barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 30 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 20 parts, spore suspension mother liquor, after two kinds of spore suspension mother liquors are mixed, adding tween, spouting liquid is 110mL/m
2, moisturizing 56h, obtain the microorganism Weeding water agent.
Embodiment 4
A kind of microbial herbicide is comprised of following each component and weight portion thereof:
Barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 28 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 24 parts, spore suspension mother liquor, 11 parts of Tween 80s.
The preparation method:
Get barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 28 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 24 parts, spore suspension mother liquor, after two kinds of spore suspension mother liquors are mixed, adding tween, spouting liquid is 150mL/m
2, moisturizing 43h, obtain the microorganism Weeding water agent.
Embodiment 5
A kind of microbial herbicide is comprised of following each component and weight portion thereof:
Barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 30 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 25 parts, spore suspension mother liquor, 12 parts of Tween 80s.
The preparation method:
Get barnyard grass transform narrow ovum chain lattice spore (
alternaria augustiovoidea) 30 parts, strains A AEC05-3 spore suspension mother liquor and Amaranthus retroflexus specially change alternaric bacteria (
a.amaranthi-3) 25 parts, spore suspension mother liquor, after two kinds of spore suspension mother liquors are mixed, adding tween, spouting liquid is 140mL/m
2, moisturizing 56h, obtain the microorganism Weeding water agent.
Claims (3)
1. a microbial herbicide, it is characterized in that: each component and weight portion thereof are:
Barnyard grass transforms narrow ovum chain lattice spore
alternaria augustiovoideastrains A AEC05-3 spore suspension mother liquor 10-35 part and Amaranthus retroflexus are specially changed alternaric bacteria
a.amaranthi-3spore suspension mother liquor 15-25 part, Tween 80 5-12 part.
2. a kind of microbial herbicide according to claim 1, it is characterized in that: described barnyard grass transforms narrow ovum chain lattice spore
alternaria augustiovoideastrains A AEC05-3 spore suspension mother liquid concentration is 1 * 10
7individual/mL; Amaranthus retroflexus is specially changed alternaric bacteria
a.amaranthi-3spore suspension mother liquid concentration is 1 * 10
7individual/mL.
3. the method for the preparation of microbial herbicide claimed in claim 1, is characterized in that: get barnyard grass and transform narrow ovum chain lattice spore
alternaria augustiovoideastrains A AEC05-3 spore suspension mother liquor 10-35 part and Amaranthus retroflexus are specially changed alternaric bacteria
a.amaranthi-3spore suspension mother liquor 15-25 part, add Tween 80, and spouting liquid is 100-150mL/m
2, moisturizing 40-60h, obtain microbial herbicide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013104072860A CN103461388A (en) | 2013-09-10 | 2013-09-10 | Microbial herbicide and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013104072860A CN103461388A (en) | 2013-09-10 | 2013-09-10 | Microbial herbicide and preparation method thereof |
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| CN103461388A true CN103461388A (en) | 2013-12-25 |
Family
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| CN2013104072860A Pending CN103461388A (en) | 2013-09-10 | 2013-09-10 | Microbial herbicide and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020091031A1 (en) | 2018-11-02 | 2020-05-07 | 日本農薬株式会社 | Pest control agent composition and method for using same |
-
2013
- 2013-09-10 CN CN2013104072860A patent/CN103461388A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 姜述君: "生防菌Alternaria amaranthi-3对反枝苋的防治效果", 《植物保护学报》 * |
| 姜述君等: "狭卵链格孢菌株AAEC05-3及其毒素对稗草的致病性", 《植物保护学报》 * |
| 杨云强等: "利用复合微生物除草剂控制稗草和反枝苋的研究", 《黑龙江八一农垦大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020091031A1 (en) | 2018-11-02 | 2020-05-07 | 日本農薬株式会社 | Pest control agent composition and method for using same |
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Application publication date: 20131225 |