CN103461359A - Preparation method for spinosad/avilamycin composite polymeric micelle insecticide - Google Patents
Preparation method for spinosad/avilamycin composite polymeric micelle insecticide Download PDFInfo
- Publication number
- CN103461359A CN103461359A CN2013104444327A CN201310444432A CN103461359A CN 103461359 A CN103461359 A CN 103461359A CN 2013104444327 A CN2013104444327 A CN 2013104444327A CN 201310444432 A CN201310444432 A CN 201310444432A CN 103461359 A CN103461359 A CN 103461359A
- Authority
- CN
- China
- Prior art keywords
- avilamycin
- pleocidin
- section material
- insecticide
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a preparation method for a spinosad/avilamycin composite polymeric micelle insecticide. The insecticide is mainly composed of the following components: in parts by weight, 10 parts of spinosad, 8-12 parts of avilamycin, 140-160 parts of a hydrophilic segment material and 140-160 parts of a hydrophobic segment material. Compared with the prior art, the obtained spinosad/avilamycin composite polymeric micelle insecticide has the characteristics of strong drug effect and long persistent period. The spinosad/avilamycin composite polymeric micelle insecticide is capable of effectively killing insects comprising lepidoptera, diptera and thysanoptera insects, and also has poisoning effect on certain specific kinds of insects in coleoptera, orthoptera, hymenoptera, isopteran, siphonaptera, dermaptera and corrodentia.
Description
Technical field
The present invention relates to a kind of preparation method of biopesticide, be specifically related to a kind of pleocidin and avilamycin composition polymer micella method for producing insecticide, be applied to agriculture field.
Background technology
Biological living and metabolite and the transgene product of the pests such as biopesticide refers to for anti-curing the disease, worm, grass, and can be made into the biogenic preparation of commodity, comprise the genetically modified plants of bacterium, virus, fungi, nematode, plant, Natural Enemies of Insects, farm antibiotics, plant growth regulator and Resistant crop smothering etc.Biopesticide is applied to the existing semicentennial history of agricultural production, but development is always comparatively slow for various reasons.In recent years, people more and more note the sustainable development of agricultural production and the coordination of human and environment, particularly difficult due to the toxic and side effect of chemical pesticide and screening novel pesticide, make enterprise and researcher start notice has been turned to research and development and the use of biopesticide.
Antibiotic is the secondary metabolite of microorganisms, for preventing and treating plant pest and weeds, is developed recently field the most rapidly.Farm antibiotics is to research and develop on the basis of the medical antibiotic development forties in 20th century, since the seventies, some have the farm antibiotics of control insect, mite, animal parasitic protozoa and worm, weeding and regulation moving plant growth function, constantly research and development out, have enlarged the application of farm antibiotics.Some antibiotic medicines are insoluble drug, while entering in pest body, be difficult to absorb, and it are short to kill the duration, and this just needs the principle of application Modern Pharmaceutics to design new formulation.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of pleocidin and avilamycin composition polymer micella method for producing insecticide.
Technical scheme: for achieving the above object, a kind of pleocidin provided by the invention and avilamycin composition polymer micella method for producing insecticide, it is comprised of following steps:
(1) at vacuum state, under 120-150 ℃ of condition, hydrophilic section material and hydrophobic section material are rendered in reaction vessel, add catalyzer, reaction 30-50 hour, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir fully and dissolve, obtain oil solution;
(4) under 40-60 ℃ of condition, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring 0.5-1 hour simultaneously, continue to stir acetone volatilized fully;
(5) filter freeze drying and get final product.
Preferably, the weight ratio of described pleocidin, avilamycin, hydrophilic section material and hydrophobic section material is: 10 parts of pleocidin, 5-15 part avilamycin, 100-200 part hydrophilic section material, 100-200 part hydrophobic section material.
Further preferably, the weight ratio of described pleocidin, avilamycin, hydrophilic section material and hydrophobic section material is: 10 parts of pleocidin, 8-12 part avilamycin, 140-160 part hydrophilic section material, 140-160 part hydrophobic section material.
Preferably, described hydrophilic section material is selected from a kind of in polyethylene glycol, polyoxyethylene, polyvidone and chitosan oligosaccharide.
Preferably, described hydrophobic section material is selected from a kind of in polypropylene, polystyrene, polyaminoacid, PLA, spermine and short-chain phospholipid.
Preferably, the molecular weight of described hydrophilic section material is 3000-10000, and the molecular weight of described hydrophobic section material is 2000-8000.
Beneficial effect: the present invention is with respect to prior art, and the pleocidin of gained and avilamycin composition polymer micella insecticide, to the person poultry harmless, do not produce poisoning to crops, and non agricultural chemical residuum is free from environmental pollution, has strong drug action, the characteristics that the lasting period is long.The insect that can effectively kill comprises Lepidoptera, diptera and thrips insect, the insect of coleoptera, orthoptera, Hymenoptera, Isoptera, Siphonaptera, Dermaptera and psocopteran some particular types is also had to certain toxic action simultaneously.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
Umber related in prescription of the present invention is parts by weight.
Embodiment 1:
Prescription: 10 parts of pleocidin, 10 parts of avilamycins, 150 polyethylene glycol, 150 parts of polypropylene
The molecular weight of polyethylene glycol is 6000, and polyacrylic molecular weight is 5000.
Above-mentioned pleocidin and avilamycin composition polymer micella method for producing insecticide, comprise the steps:
(1) at vacuum state, under 140 ℃ of conditions, polyethylene glycol and polypropylene are rendered in reaction vessel, add catalyzer, react 40 hours, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir fully and dissolve, obtain oil solution;
Under (4) 50 ℃ of conditions, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring is 0.8 hour simultaneously, continues to stir acetone is volatilized fully;
(5) filter freeze drying and get final product.
The insecticide made in the present embodiment, the kill pests effect is strong, and the lasting period is permanent.
Embodiment 2:
Prescription: 10 parts of pleocidin, 5 parts of avilamycins, 100 parts of polyoxyethylene, 100 parts of polystyrene
Polyoxyethylated molecular weight is 3000, and the molecular weight of polystyrene is 2000.
Above-mentioned pleocidin and avilamycin composition polymer micella method for producing insecticide, comprise the steps:
(1) at vacuum state, under 150 ℃ of conditions, polyoxyethylene and polystyrene are rendered in reaction vessel, add catalyzer, react 50 hours, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir fully and dissolve, obtain oil solution;
Under (4) 60 ℃ of conditions, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring is 1 hour simultaneously, continues to stir acetone is volatilized fully;
(5) filter freeze drying and get final product.
The insecticide made in the present embodiment, the kill pests effect is strong, and the lasting period is permanent.
Embodiment 3:
Prescription: 10 parts of pleocidin, 15 parts of avilamycins, 200 parts of polyvidone, 200 parts of polyaminoacid
The molecular weight of polyvidone is 10000, and the molecular weight of polyaminoacid is 8000.
Above-mentioned pleocidin and avilamycin composition polymer micella method for producing insecticide, comprise the steps:
(1) at vacuum state, under 120 ℃ of conditions, polyvidone and polyaminoacid are rendered in reaction vessel, add catalyzer, react 30 hours, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir fully and dissolve, obtain oil solution;
Under (4) 40 ℃ of conditions, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring is 0.5 hour simultaneously, continues to stir acetone is volatilized fully;
(5) filter freeze drying and get final product.
The insecticide made in the present embodiment, the kill pests effect is strong, and the lasting period is permanent.
Embodiment 4:
Prescription: 10 parts of pleocidin, 8 parts of avilamycins, 140 parts of chitosan oligosaccharides, 140 parts of PLAs
The molecular weight of chitosan oligosaccharide is 5000, and the molecular weight of PLA is 3000.
Above-mentioned pleocidin and avilamycin composition polymer micella method for producing insecticide, comprise the steps:
(1) at vacuum state, under 130 ℃ of conditions, chitosan oligosaccharide and PLA are rendered in reaction vessel, add catalyzer, react 35 hours, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir fully and dissolve, obtain oil solution;
Under (4) 45 ℃ of conditions, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring is 0.5 hour simultaneously, continues to stir acetone is volatilized fully;
(5) filter freeze drying and get final product.
The insecticide made in the present embodiment, the kill pests effect is strong, and the lasting period is permanent.
Embodiment 5:
Prescription: 10 parts of pleocidin, 12 parts of avilamycins, 160 parts of polyethylene glycol, 160 parts of spermine
The molecular weight of polyethylene glycol is 8000, and the molecular weight of spermine is 6000.
Above-mentioned pleocidin and avilamycin composition polymer micella method for producing insecticide, comprise the steps:
(1) at vacuum state, under 145 ℃ of conditions, polyethylene glycol and spermine are rendered in reaction vessel, add catalyzer, react 45 hours, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir fully and dissolve, obtain oil solution;
Under (4) 55 ℃ of conditions, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring is 1 hour simultaneously, continues to stir acetone is volatilized fully;
(5) filter freeze drying and get final product.
The insecticide made in the present embodiment, the kill pests effect is strong, and the lasting period is permanent.
Experimental example gained insecticide of the present invention the results are shown in Table 1 to the effect experiment of diamondback moth larvae
Test is the diamond-back moth of raising in indoor continuous generation for examination worm source, and test method adopts the blade infusion process;
Common pleocidin supensoid agent, common Avermectin supensoid agent, common pleocidin and Avermectin supensoid agent all refer to formulation method routinely, add the made supensoid agent of conventional auxiliary material, are made as respectively A control group, B control group and C control group;
The recommended dose that drug dose is the pleocidin field control;
Median lethal concentration (LC
50): be illustrated in experiment and make the lethal required drug concentration of half tested animal (insect), with mg/m
3mean.Its value is less, shows that the toxicity of medicine is larger, and effect is stronger;
Lethality after medicine: refer to after drug administration that, after certain hour, death toll accounts for the percentage of total test number.
The effect experiment result of table 1 gained insecticide of the present invention to diamondback moth larvae
Annotate: with A control group, B control group and C control group, compare,
*p<0.05
As seen from the above table, the C control group is compared with A control group, B control group, after median lethal concentration, medicine after 3 days lethality and medicine 7 days lethality all there is no marked change.And the pleocidin of gained of the present invention and Avermectin composition polymer micella insecticide are compared with three control groups, LC
50obviously reduce, insecticidal effect improves; After medicine, 7 days lethality after 3 days lethality and medicine, compare with three control groups, obviously improves, and illustrates that the insecticide desinsection lasting period of the present invention is permanent.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, in scope disclosed by the invention, carries out some improvement and modification, also should be considered as protection scope of the present invention.
Claims (6)
1. a pleocidin and avilamycin composition polymer micella method for producing insecticide, it is characterized in that: it is made by following steps:
(1) at vacuum state, under 120-150 ℃ of condition, hydrophilic section material and hydrophobic section material are rendered in reaction vessel, add catalyzer, reaction 30-50 hour, obtain amphipathic nature block polymer;
(2) above-mentioned amphipathic nature block polymer is soluble in water, obtain the aqueous solution;
(3) pleocidin and avilamycin are added in acetone, stir and make to dissolve fully, obtain oil solution;
(4) under 40-60 ℃ of condition, above-mentioned oil solution is slowly joined in the described aqueous solution, vigorous stirring 0.5-1 hour simultaneously, continue to stir acetone volatilized fully;
(5) filter freeze drying and get final product.
2. a kind of pleocidin according to claim 1 and avilamycin composition polymer micella method for producing insecticide, it is characterized in that: the weight ratio of described pleocidin, avilamycin, hydrophilic section material and hydrophobic section material is: 10 parts of pleocidin, 5-15 part avilamycin, 100-200 part hydrophilic section material, 100-200 part hydrophobic section material.
3. a kind of pleocidin according to claim 2 and avilamycin composition polymer micella method for producing insecticide, it is characterized in that: the weight ratio of described pleocidin, avilamycin, hydrophilic section material and hydrophobic section material is: 10 parts of pleocidin, 8-12 part avilamycin, 140-160 part hydrophilic section material, 140-160 part hydrophobic section material.
4. a kind of pleocidin according to claim 1 and avilamycin composition polymer micella method for producing insecticide is characterized in that: described hydrophilic section material is selected from a kind of in polyethylene glycol, polyoxyethylene, polyvidone and chitosan oligosaccharide.
5. a kind of pleocidin according to claim 1 and avilamycin composition polymer micella method for producing insecticide is characterized in that: described hydrophobic section material is selected from a kind of in polypropylene, polystyrene, polyaminoacid, PLA, spermine and short-chain phospholipid.
6. a kind of pleocidin according to claim 1 and avilamycin composition polymer micella method for producing insecticide, it is characterized in that: the molecular weight of described hydrophilic section material is 3000-10000, the molecular weight of described hydrophobic section material is 2000-8000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310444432.7A CN103461359B (en) | 2013-09-26 | 2013-09-26 | A kind of pleocidin and avilamycin composition polymer micella method for producing insecticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310444432.7A CN103461359B (en) | 2013-09-26 | 2013-09-26 | A kind of pleocidin and avilamycin composition polymer micella method for producing insecticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103461359A true CN103461359A (en) | 2013-12-25 |
| CN103461359B CN103461359B (en) | 2015-10-28 |
Family
ID=49786602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310444432.7A Active CN103461359B (en) | 2013-09-26 | 2013-09-26 | A kind of pleocidin and avilamycin composition polymer micella method for producing insecticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103461359B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172380A (en) * | 2016-07-14 | 2016-12-07 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of the blade face controlled carrier micelle of affinity |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524581A (en) * | 2003-02-25 | 2004-09-01 | 吴建梅 | Polymer micelle medicine-carrying system |
| CN100998870A (en) * | 2006-11-27 | 2007-07-18 | 涂家生 | Stable polymer micelle medicine carrging system |
| CN101389215A (en) * | 2006-01-10 | 2009-03-18 | 英诺瓦佛姆技术有限公司 | Pesticide delivery system |
| CN101755820A (en) * | 2009-10-22 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Pesticide suspension concentrate containing pleocidin and preparation method thereof |
-
2013
- 2013-09-26 CN CN201310444432.7A patent/CN103461359B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524581A (en) * | 2003-02-25 | 2004-09-01 | 吴建梅 | Polymer micelle medicine-carrying system |
| CN101389215A (en) * | 2006-01-10 | 2009-03-18 | 英诺瓦佛姆技术有限公司 | Pesticide delivery system |
| CN100998870A (en) * | 2006-11-27 | 2007-07-18 | 涂家生 | Stable polymer micelle medicine carrging system |
| CN101755820A (en) * | 2009-10-22 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Pesticide suspension concentrate containing pleocidin and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172380A (en) * | 2016-07-14 | 2016-12-07 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of the blade face controlled carrier micelle of affinity |
| CN106172380B (en) * | 2016-07-14 | 2019-02-22 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of the controllable carrier micelle of blade face compatibility |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103461359B (en) | 2015-10-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102524319B (en) | Multiple-effect nanoscale biopesticide water aqua and use thereof | |
| CN103651607B (en) | A kind of vegetables biopesticide and preparation method thereof | |
| CN103651615B (en) | A kind of biological insecticides containing lycopene and preparation method thereof | |
| CN103651594B (en) | A kind of composite biological insecticide | |
| WO2019140990A1 (en) | Miticide and application thereof | |
| CN105165844A (en) | Fluensulfone-containing nematode killing composition | |
| CN103461360B (en) | The preparation method of a kind of Avermectin and griseofulvin composite pesticidal nanoemulsion | |
| CN107258826A (en) | It is a kind of to reduce the biological insecticides of Cucumber Pests And Diseases | |
| CN103478145A (en) | Spinosyn and avermectin composite polymeric micellar pesticide | |
| CN102948434A (en) | Aquaculture sterilization parasiticide | |
| CN103461359B (en) | A kind of pleocidin and avilamycin composition polymer micella method for producing insecticide | |
| CN1957705A (en) | Composite preparation of pesticide between triflumuron and antibiotic insecticide | |
| CN103478144B (en) | A kind of Avermectin and griseofulvin composite pesticidal nanoemulsion | |
| CN103355322A (en) | Compound biological insecticide and application thereof | |
| CN103461336B (en) | A kind of pleocidin and griseofulvin composite microcapsule insecticide | |
| CN111265538B (en) | Application of periplocin in preparing medicine for killing fish ectoparasite | |
| CN108208034A (en) | A kind of desinsection and the biological insecticides of fertilizer efficiency one and preparation method thereof | |
| CN103461335B (en) | A kind of pleocidin and griseofulvin composite microcapsule method for producing insecticide | |
| CN103931649B (en) | The Pesticidal combination of emamectin-benzoate and cholrempenthrin | |
| CN102988462B (en) | Chinese medicinal preparation for controlling parasites of aquatic animal as well as preparation method and application thereof | |
| CN106259379B (en) | A kind of nematicidal composition containing trifluorobutene base class compound | |
| KR100784917B1 (en) | Scuticociliates insecticide composition using butyraldehyde | |
| Adetuyi et al. | Application of Plant-Based Nanobiopesticides as Sanitizers | |
| CN104351218B (en) | Microcapsule suspension containing nimbin and chitosan oligosaccharide as effective components | |
| CN104206406B (en) | A kind of nano-emulsion insecticide and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 325200 Wenzhou City, Ruian City, fairy street, the village of the first Village No. 115 Applicant after: Bian Jialin Address before: 212100, No. 4, No. 54, Henderson Pacific Business Center, Dantu New District, Jiangsu, Zhenjiang Applicant before: Bian Jialin |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 212100 ZHENJIANG, JIANGSU PROVINCE TO: 325200 WENZHOU, ZHEJIANG PROVINCE |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230425 Address after: 714000 West Section of Guangda Road, Economic and Technological Development Zone, Guanchi Town, Dali County, Weinan City, Shaanxi Province Patentee after: Shaanxi Gelida Biotechnology Co.,Ltd. Address before: 325200 No. 115 Tong Tou village, Xian Jiang Street, Ruian, Wenzhou, Zhejiang Patentee before: Bian Jialin |
|
| TR01 | Transfer of patent right |