CN103461142A - Blueberry subculture multiplication medium - Google Patents
Blueberry subculture multiplication medium Download PDFInfo
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- CN103461142A CN103461142A CN 201310457713 CN201310457713A CN103461142A CN 103461142 A CN103461142 A CN 103461142A CN 201310457713 CN201310457713 CN 201310457713 CN 201310457713 A CN201310457713 A CN 201310457713A CN 103461142 A CN103461142 A CN 103461142A
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- blueberry
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Abstract
The invention discloses a subculture multiplication medium for blueberry tissue culture. The blueberry subculture multiplication medium comprises, by weight, 1g/L of peters professional number 12, 0.5-1mg/L of ZT, 20g/L of white sugar, 7-8g/L of agar powder, 0.35g/L of magnesium sulfate heptahydrate, 0.45g/L of calcium chloride dehydrate, microelement containing WPM, ferric salt, organic components and the balance water. The pH value of the medium is 5.0-5.3. The medium has the advantages of reducing the cost in the blueberry tissue culture process, increasing multiplication multiples, simplifying the medium manufacturing process and indicating subculture time.
Description
Technical field
The invention belongs to biological field, in particular to a kind of blueberry shoot proliferation medium.
Background technology
Blueberry (Blueberry), have another name called cowberry, blue berry, belongs to Ericaceae (Ericaceae), the perennial fruit shrubs of Vaccinium (Vaccinium).Blueberry originates in North America, and approximately there is kind more than 400 in the whole world, and approximately 28 mutation of 91 kinds of China mainly are distributed in Changbaishan area, the large Xiaoxinanlin Mountains and Southwest Mountainous Areas, and there is a small amount of distribution the Yangtze river basin, is the indicative plant of acid ground, is applicable to the south China cultivation.
Blueberry has higher economic worth and wide DEVELOPMENT PROSPECT, its fruit navy blue, be rich in anthocyanin, low sugar, low fat, oxidation resistance is listed as first of all fruits, vegetables, blueberry contain prevent that cerebral nerve is old and feeble, strengthen cardiac function, improving eyesight and the unique effects material such as anticancer, it is in the prevention cancer of the uterus, the treatment children's diarrhae, the amblyopia of improving the people has unique effect.Classified as one of the mankind's five large healthy food by international food and agricultural organization, can be rated as " king of third generation in the world fruit ".
The blueberry seedling-cultivating method mainly contains green wood cutting, seed grows directly from seeds and breeds and group culturation rapid propagating technology.Wherein the method for tissue culture reproduction speed is fast, is suitable for the batch production large-scale breeding of improved seeds.In addition, group training seedling can be realized blueberry nursery stock detoxification production, and fundamentally preventing virus is critically ill and is done harm to and the insect pest generation, guarantees production safety.Compare and the above two, the tissue-culturing rapid propagation blueberry is not subject to time restriction, and a large amount of high-quality blueberry nursery stocks can uninterruptedly be provided, and can keep the maternal advantages such as merit, be the main mode of production of current blueberry seedling.But the blueberry tissue culture medium is essentially WPM, improvement WPM and MS etc. at present, it is made into by experiment level chemical reagent, and large-scale industrialized production cost is very high.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of blueberry shoot proliferation medium, and this medium should have the cost reduced in the blueberry tissue culture process, increases breeding rate, simplifies the characteristics of medium manufacturing process and indication Subculture Time.
Technical scheme provided by the invention is: a kind of blueberry shoot proliferation medium, comprising composition and content be:
Flower is No. 12 1 g/L the more; ZT 0.5-1 mg/L; White sugar 20 g/L; Agar powder 7-8 g/L; Epsom salt 0.35 g/L; Calcium chloride dihydrate 0.45 g/L; Comprise trace element, molysite and organic principle, all the other are running water, pH 5.0-5.3;
Described trace element is: boric acid 6 mg/L, cupric sulfate pentahydrate 0.25 mg/L, four water manganese sulphate 22 mg/L, Sodium Molybdate Dihydrate 0.25 mg/L, white vitriol 8.5 mg/L;
Described molysite is: two water sodium ethylene diamine tetracetate 37 mg/L, ferrous sulfate heptahydrate 28 mg/L;
Described organic principle is: inositol 100 mg/L, nicotinic acid 0.5 mg/L, glycine 2.0 mg/L, vitamin B6 0.5 mg/L, vitamin B1 0.5 mg/L;
Described ZT be 6-trans-4-hydroxy-3-methyl-but-2-ene base aminopurine (please examine this name);
Described flower the more is comprised of full nitrogen (1.89% ammonium nitrogen, 8.68% nitrate nitrogen, 4.43% urea nitrogen), water-soluble phosphoric anhydride, water-soluble potassium for No. 12, proportioning is: 15:10:30 also comprises boron 0.0068%, copper (chelating state) 0.0036%, iron (chelating state) 0.05%, manganese (chelating state) 0.025%, molybdenum 0.0009%, zinc (chelating state) 0.0025%.
This medium sucrose used is edible sugar.
This medium water used is running water.
The pH value of this medium is regulated with 1 mol/L NaOH or HCl.
This medium just needs subculture after blueness is decorporated.
Medium of the present invention has the following advantages: (1) made medium can make the propagation multiple of blueberry tissue culture process increase.(2) due to this fill a prescription with the flower the more replaced macroelement No. 12, saved greatly production cost, can carry out large-area popularization.(3) water used is civil running water, and sucrose is edible sugar, and wide material sources are cheap.(4) preparation method is easy.(5) in Subculture, after the medium blueness is decorporated, but with regard to subculture.
Embodiment
By following examples, the present invention is described in further detail, but content of the present invention is not limited to this.
Embodiment 1:
Choose Bridges tower group training seedling as the subculture material, it is cultivated on blueberry shoot proliferation medium, the composition of medium and content are: flower is No. 12 1 g/L the more; ZT 0.5 mg/L; Edible sugar 20 g/L; Agar powder 7 g/L; Epsom salt 0.35 g/L; Calcium chloride dihydrate 0.45 g/L; The trace element that comprises WPM, molysite and organic principle, all the other are running water, pH 5.0.
Trace element is: boric acid 6 mg/L, cupric sulfate pentahydrate 0.25 mg/L, four water manganese sulphate 22 mg/L, Sodium Molybdate Dihydrate 0.25 mg/L, white vitriol 8.5 mg/L;
Molysite is: two water sodium ethylene diamine tetracetate 37 mg/L, ferrous sulfate heptahydrate 28 mg/L;
Organic principle is: inositol 100 mg/L, nicotinic acid 0.5 mg/L, glycine 2.0 mg/L, vitamin B6 0.5 mg/L, vitamin B1 0.5 mg/L;
Medium after blueness is decorporated just by its subculture on identical medium.
After subculture is cultivated 30 d, in this medium, the growth coefficient of blueberry tissue culture seedling reaches 12-20 doubly, and in the WPM medium, is 3-6 times; After subculture is cultivated 45 d, more than 90% blueberry tissue culture seedling is 10 cm, and plant is all very sturdy; In 6 months process of growths, growth coefficient and robust plant degree all change, and have good stability.
Embodiment 2:
Choose Bridges tower group training seedling as the subculture material, it is cultivated on blueberry shoot proliferation medium, the composition of medium and content are: flower is No. 12 1 g/L the more; ZT 1 mg/L; Edible sugar 20 g/L; Agar powder 8 g/L; Epsom salt 0.35 g/L; Calcium chloride dihydrate 0.45 g/L; The trace element that comprises WPM, molysite and organic principle, all the other are running water, pH 5.3.
Trace element is: boric acid 6 mg/L, cupric sulfate pentahydrate 0.25 mg/L, four water manganese sulphate 22 mg/L, Sodium Molybdate Dihydrate 0.25 mg/L, white vitriol 8.5 mg/L;
Molysite is: two water sodium ethylene diamine tetracetate 37 mg/L, ferrous sulfate heptahydrate 28 mg/L;
Organic principle is: inositol 100 mg/L, nicotinic acid 0.5 mg/L, glycine 2.0 mg/L, vitamin B6 0.5 mg/L, vitamin B1 0.5 mg/L;
Medium after blueness is decorporated just by its subculture on identical medium.
After subculture is cultivated 30 d, in this medium, the growth coefficient of blueberry tissue culture seedling reaches 12-20 doubly, and in the WPM medium, is 3-6 times; After subculture is cultivated 45 d, more than 90% blueberry tissue culture seedling is 10 cm, and plant is all very sturdy; In 6 months process of growths, growth coefficient and robust plant degree all change, and have good stability.
The above is the preferred embodiments of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite that does not break away from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. a blueberry shoot proliferation medium, comprising composition and content be:
Flower is No. 12 0.8-1.2 g/L the more; ZT 0.5-1 mg/L; White sugar 20 g/L; Agar powder 7-8 g/L; Epsom salt 0.35 g/L; Calcium chloride dihydrate 0.45 g/L; Trace element, molysite and organic principle; All the other are running water, pH 5.0-5.3;
Described trace element is: boric acid 6 mg/L, cupric sulfate pentahydrate 0.25 mg/L, four water manganese sulphate 22 mg/L, Sodium Molybdate Dihydrate 0.25 mg/L, white vitriol 8.5 mg/L;
Described molysite is: two water sodium ethylene diamine tetracetate 37 mg/L, ferrous sulfate heptahydrate 28 mg/L;
Described organic principle is: inositol 100 mg/L, nicotinic acid 0.5 mg/L, glycine 2.0 mg/L, vitamin B6 0.5 mg/L, vitamin B1 0.5 mg/L;
Described ZT be 6-trans-4-hydroxy-3-methyl-but-2-ene base aminopurine;
Described flower the more is commercially available for No. 12 or is comprised of full nitrogen (1.89% ammonium nitrogen, 8.68% nitrate nitrogen, 4.43% urea nitrogen), water-soluble phosphoric anhydride, water-soluble potassium, proportioning is: 15:10:30 also comprises boron 0.0068%, copper (chelating state) 0.0036%, iron (chelating state) 0.05%, manganese (chelating state) 0.025%, molybdenum 0.0009%, zinc (chelating state) 0.0025%.
2. a kind of blueberry shoot proliferation medium according to claim 1, is characterized in that this medium sucrose used is edible sugar.
3. a kind of blueberry shoot proliferation medium according to claim 1, is characterized in that this medium water used is running water.
4. a kind of blueberry shoot proliferation medium according to claim 1, is characterized in that the pH value of this medium is regulated with 1 mol/L NaOH or HCl.
5. the application of blueberry shoot proliferation medium according to claim 1 in cultivating the blueberry tissue culture seedling, is characterized in that carrying out the subculture cultivation in cultivating process until medium after blueness is decorporated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310457713 CN103461142A (en) | 2013-09-30 | 2013-09-30 | Blueberry subculture multiplication medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310457713 CN103461142A (en) | 2013-09-30 | 2013-09-30 | Blueberry subculture multiplication medium |
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| CN103461142A true CN103461142A (en) | 2013-12-25 |
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| CN 201310457713 Pending CN103461142A (en) | 2013-09-30 | 2013-09-30 | Blueberry subculture multiplication medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012409A (en) * | 2014-06-09 | 2014-09-03 | 赵兰 | Multiplication subculture culture medium for cowberry blueberries |
| CN104082142A (en) * | 2014-07-02 | 2014-10-08 | 江苏农林职业技术学院 | Proliferation medium for Sierra cowberry blueberry tissue culture and preparation method thereof |
-
2013
- 2013-09-30 CN CN 201310457713 patent/CN103461142A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012409A (en) * | 2014-06-09 | 2014-09-03 | 赵兰 | Multiplication subculture culture medium for cowberry blueberries |
| CN104082142A (en) * | 2014-07-02 | 2014-10-08 | 江苏农林职业技术学院 | Proliferation medium for Sierra cowberry blueberry tissue culture and preparation method thereof |
| CN104082142B (en) * | 2014-07-02 | 2015-10-14 | 江苏农林职业技术学院 | A kind of happiness carrys out blueberry tissue and cultivates proliferated culture medium and preparation method thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131225 |
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| WD01 | Invention patent application deemed withdrawn after publication |