CN103416217A - Artificial fruiting method of wild Agrocybe aegerita - Google Patents
Artificial fruiting method of wild Agrocybe aegerita Download PDFInfo
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- CN103416217A CN103416217A CN2012101790713A CN201210179071A CN103416217A CN 103416217 A CN103416217 A CN 103416217A CN 2012101790713 A CN2012101790713 A CN 2012101790713A CN 201210179071 A CN201210179071 A CN 201210179071A CN 103416217 A CN103416217 A CN 103416217A
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- poplar
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- agrocybe aegerita
- aseptic
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Abstract
The invention provides an artificial fruiting method of wild Agrocybe aegerita. The method includes collecting wild strains, producing grain pure strains, collecting poplar branches, soaking, controlling moisture and adjusting humidity, drilling holes and inoculating, shielding greenhouse and germinating, burying and shielding and realizing the step of fruiting. According to the method, growth process of the wild Agrocybe aegerita is restored under the artificial condition, and the Agrocybe aegerita, having good qualities as wild Agrocybe aegerita, is crisp, good in taste, soft and delicate, free of ribs and residue.
Description
[technical field]
Present technique belongs to field of ecological agricultural, and by manual simulation's natural conditions, but Artificial Control ground produces high-quality wild edible fungus.
[background technology]
Current Edible Fungi mainstream technology is to be main medium with cotton seed hulls, and plastic sack is made the bacterium bag, and high-temperature sterilization is inoculated under gnotobasis, and temperature control is sent out bacterium, the booth fruiting.Because main composts or fertilisers of cultivating is cotton seed hulls, send out bacterium speed fast, the fruiting time is short, but fiber is many, poor quality.By method of the present invention, simulation wild environment and growth conditions, make artificial edible mushroom and the wild edible fungus of producing have equal quality.
[summary of the invention]
The invention provides the artificial fruiting method of a kind of wild Agrocybe aegerita.
The present invention is achieved in that
1, gather wild-type strain: get wild Agrocybe aegerita fruit body, indoor at aseptic inoculation, wind 30 minutes, carry out disinfection with 75% alcohol antithetical phrase solid object surface, with hand, break fruit body into two with one's hands, with aseptic nipper, get fresh meat bacteria organization, at aseptic PDA medium, in vitro inoculate, place in 25 ℃ of constant incubators, cultivate 28-32 days under dark condition, obtain the pure white dense wild virus-free pure culture of mycelia;
2, make the wheat pure culture: get without broken grain, full wheat, boil 28-32 minute in boiling water, pull, dry surperficial moisture content out, admix 0.5% gesso, in the vial of packing into, after sealing, under 121 ℃ of steam, sterilizing 90 minutes, after taking-up, be placed on aseptic inoculation indoor, naturally cool to below 25 ℃, on aseptic working platform, the access test tube strains, be placed under room temperature 24-26 ℃ condition dark culturing 28-32 days, treat that mycelia covers with, become the wheat pure culture;
3, gather poplar-branch: the poplar-branch of unclogging and readjusting of choosing natural air drying;
4, immersion treatment: by air-dry poplar-branch, steep in the lime clear water, soak 45-50 hour, pull out.
5, control water damping: the moisture content on poplar-branch surface is drained, reach 120% of dry weight and get final product;
6, boring inoculation: on poplar-branch, the hole of drill diameter 8-12mm, fill in cultured wheat pure culture in hole, with batten, seals hole, completes inoculation;
7, cool canopy is sent out bacterium: will connect the poplar-branch of planting, and put into cool canopy, temperature is controlled between 23-27 ℃, and maintenance relative moisture is 85%-95%, and bacteria developing period is 26-34 days, treats that white healthy mycelium appears in the poplar-branch surface, completes and sends out a bacterium;
8, the buried fruiting that shades: the poplar-branch by sending out bacterium good, in horizontal placing into the soil, shade 70%, maintenance relative moisture is 70%-90%, alternation of wetting and drying gets final product fruiting after first quarter moon.
The invention has the beneficial effects as follows: the present invention gathers the wild Agrocybe aegerita in Altai Mountains and extracts bacterial classification, utilize branch of poplar row culture composts or fertilisers of cultivating, the mode of inoculating by boring, the willow branch that covers with mycelia is buried after, simulate wild Agrocybe aegerita seeding process or simulation woods environment is sent out bacterium; The present invention, under artificial condition, reduces wild Agrocybe aegerita process of growth, and obtain that eating of wild Agrocybe aegerita equal quality is clear and melodious, mouthfeel good, soft, delicate, there is no the muscle silk, without the wild Agrocybe aegerita of slag product.
[embodiment]
The present invention is achieved in that
1, gather wild-type strain: during annual April 10 to May 10, growing poplar out of office vitellarium, the selection color and luster is greyish black, shape is as stacked fan-shaped, the wild Agrocybe aegerita fruit body that cap thickness 10mm is above, under adopting in the time of temperature 12-18 ℃, in 6 hours, be transported to aseptic inoculation with antistaling box indoor, be placed on superclean bench, wind 30 minutes, with 75% alcohol antithetical phrase solid object surface, carry out disinfection, break fruit body into two with one's hands with hand, get not the unsalted surface with EDGE CONTACT with aseptic nipper, get meat bacteria organization, at aseptic PDA medium, in vitro inoculate, be placed in 25 ℃ of constant incubators, cultivate 28-32 days under dark condition, get growing way strong, the pure white dense test tube of mycelia, enlarge cultivation, obtain wild virus-free pure culture,
2, make the wheat pure culture: get without broken grain, full wheat, boil 28-32 minute in boiling water, pull, dry surperficial moisture content out, admix 0.5% gesso, in the 500ml vial of packing into, after sealing, under 121 ℃ of steam, sterilizing 90 minutes, after taking-up, be placed on aseptic inoculation indoor, naturally cool to below 25 ℃, on aseptic working platform, the access test tube strains, be placed under room temperature 22-28 ℃ condition dark culturing 28-32 days, treat that mycelia covers with, become the wheat pure culture;
3, gather poplar-branch: after annual autumn willow fallen leaves, choose the poplar-branch of unclogging and readjusting, pile wide 3m after felling, high 2m, the rick that length is not limit, make its natural air drying, as the main cultivation poplar-branch of producing;
4, immersion treatment: mid-April, by air-dry poplar-branch, use binder, be bundled into long 100cm, the circular faggot of diameter 30cm, closely engage the middle branch of faggot, steeps in the lime clear water, soaks 45-50 hour, pulls out;
5, control water damping: faggot surface moisture content is drained, reach 120% of dry weight and get final product;
6, boring inoculation: on poplar-branch, the hole of drill diameter 8-12mm, fill in cultured wheat kind in hole, with batten, seals hole, completes inoculation;
7, cool canopy is sent out bacterium: will connect the faggot of planting, and put into cool canopy, temperature is controlled between 23-27 ℃, and maintenance relative moisture is 85%-95%, and bacteria developing period is 26-34 days, treats that white healthy mycelium appears in the faggot surface, completes and sends out a bacterium;
8, the buried fruiting that shades: by the faggot of sending out bacterium good, horizontal 1/3 places into the soil, and shades 70%, and maintenance relative moisture is 70%-90%, and alternation of wetting and drying gets final product fruiting after first quarter moon.
Claims (1)
1. the artificial fruiting method of a kind of wild Agrocybe aegerita of the present invention, realization through the following steps:
A, collection wild-type strain: get wild Agrocybe aegerita fruit body, indoor at aseptic inoculation, wind 30 minutes, carry out disinfection with 75% alcohol antithetical phrase solid object surface, with hand, break fruit body into two with one's hands, with aseptic nipper, get fresh meat bacteria organization, at aseptic PDA medium, in vitro inoculate, place in 25 ℃ of constant incubators, cultivate 28-32 days under dark condition, obtain the pure white dense wild virus-free pure culture of mycelia;
B, make the wheat pure culture: get without broken grain, full wheat, boil 28-32 minute in boiling water, pull, dry surperficial moisture content out, admix 0.5% gesso, in the vial of packing into, after sealing, under 121 ℃ of steam, fiery bacterium 90 minutes, after taking-up, be placed on aseptic inoculation indoor, naturally cool to below 25 ℃, on aseptic working platform, the access test tube strains, be placed under room temperature 24-26 ℃ condition dark culturing 28-32 days, treat that mycelia covers with, become the wheat pure culture;
C, collection poplar-branch: the poplar-branch of unclogging and readjusting of choosing natural air drying;
D, immersion treatment: by air-dry poplar-branch, steep in the lime clear water, soak 45-50 hour, pull out;
E, the damping of control water: the moisture content on poplar-branch surface is drained, reach 120% of dry weight and get final product;
F, boring inoculation: on poplar-branch, the hole of drill diameter 8-12mm, fill in cultured wheat pure culture in hole, with batten, seals hole, completes inoculation;
G, cool canopy are sent out bacterium: will connect the poplar-branch of planting, and put into cool canopy, room temperature is incubated between 23-27 ℃, and maintenance relative moisture is 85%-95%, and bacteria developing period is 26-34 days, treats that white healthy mycelium appears in the poplar-branch surface, completes and sends out a bacterium;
H, the buried fruiting that shades: the poplar-branch by sending out bacterium good, in horizontal placing into the soil, shade 70%, maintenance relative moisture is 70%-90%, alternation of wetting and drying got final product fruiting after 15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210179071.3A CN103416217B (en) | 2012-05-25 | 2012-05-25 | The artificial fruiting method of a kind of wild Agrocybe aegerita |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210179071.3A CN103416217B (en) | 2012-05-25 | 2012-05-25 | The artificial fruiting method of a kind of wild Agrocybe aegerita |
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| CN103416217A true CN103416217A (en) | 2013-12-04 |
| CN103416217B CN103416217B (en) | 2015-09-02 |
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| CN201210179071.3A Expired - Fee Related CN103416217B (en) | 2012-05-25 | 2012-05-25 | The artificial fruiting method of a kind of wild Agrocybe aegerita |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105580547A (en) * | 2015-12-28 | 2016-05-18 | 刘青玉 | Matrix grid rod-insertion sowing combination method for city flowers |
Citations (3)
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|---|---|---|---|---|
| CN1247019A (en) * | 1998-09-04 | 2000-03-15 | 郑向阳 | Method for cultivating spawns of Maili mushroom |
| CN101366347A (en) * | 2008-09-23 | 2009-02-18 | 徐瑞安 | Method for cultivating Agrocybe aegerita with alive stumpage |
| CN101637100A (en) * | 2009-09-01 | 2010-02-03 | 南阳天冠种业有限公司 | Artificial acclimation method of agrocybe prauox fayod |
-
2012
- 2012-05-25 CN CN201210179071.3A patent/CN103416217B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1247019A (en) * | 1998-09-04 | 2000-03-15 | 郑向阳 | Method for cultivating spawns of Maili mushroom |
| CN101366347A (en) * | 2008-09-23 | 2009-02-18 | 徐瑞安 | Method for cultivating Agrocybe aegerita with alive stumpage |
| CN101637100A (en) * | 2009-09-01 | 2010-02-03 | 南阳天冠种业有限公司 | Artificial acclimation method of agrocybe prauox fayod |
Non-Patent Citations (3)
| Title |
|---|
| 周会明等: "《田头菇属真菌研究进展》", 《中国食用菌》, 15 November 2009 (2009-11-15), pages 3 - 8 * |
| 姜涛等: "《黑木耳段木栽培》", 《特种经济动植物》, 31 July 2007 (2007-07-31), pages 44 * |
| 许旭萍等: "《柱状田头菇的栽培及生物化学研究概况》", 《药物生物技术》, 30 June 2003 (2003-06-30), pages 117 - 120 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105580547A (en) * | 2015-12-28 | 2016-05-18 | 刘青玉 | Matrix grid rod-insertion sowing combination method for city flowers |
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| CN103416217B (en) | 2015-09-02 |
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Correction item: Applicant Correct: Qinghe County three sharp agriculture and animal husbandry scientific and technological development Co., Ltd False: Xinjiang Qinghe County Sanli Agriculture Animal Husbandry Technology Development Co., Ltd. Number: 49 Volume: 29 |
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| CI03 | Correction of invention patent |
Correction item: Applicant Correct: Qinghe County three sharp agriculture and animal husbandry scientific and technological development Co., Ltd False: Xinjiang Qinghe County Sanli Agriculture Animal Husbandry Technology Development Co., Ltd. Number: 49 Page: The title page Volume: 29 |
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