CN103411915A - Infrared spectroscopy identification method of traditional Chinese medicine preparation-Jinshuibao capsule - Google Patents
Infrared spectroscopy identification method of traditional Chinese medicine preparation-Jinshuibao capsule Download PDFInfo
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- CN103411915A CN103411915A CN2013103196601A CN201310319660A CN103411915A CN 103411915 A CN103411915 A CN 103411915A CN 2013103196601 A CN2013103196601 A CN 2013103196601A CN 201310319660 A CN201310319660 A CN 201310319660A CN 103411915 A CN103411915 A CN 103411915A
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- paecilomyces hepiall
- hepiall chen
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- jinshuibao
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Abstract
The invention relates to an infrared spectroscopy identification method of a traditional Chinese medicine preparation-Jinshuibao capsule. The method comprises the following steps: a, taking contents of Jinshuibao capsules, and drying for 2-4 hours at the temperature of 60-80 DEG C; b, uniformly mixing the dried contents of Jinshuibao capsules and potassium bromide at a ratio of 1 to 100, and tabletting; c, adopting a Fourier transform infrared spectrometer to test samples, wherein the resolution ratio is 4 cm<-1>, the scanning time is 32, and the scanning wavelength is 400-4,000 cm<-1>; d, adopting OPUS analysis software to process the infrared spectrograms to obtain dried infrared spectrograms of the contents of the Jinshuibao capsules; e, comparing the dried infrared spectrograms of the contents of the Jinshuibao capsule with a standard infrared spectrogram of the Jinshuibao capsule, wherein samples with same characteristic peaks existing between the 1,400-1,700 cm<-1> peak and 600-1,200 cm<-1> peak are the Jinshuibao capsules.
Description
Technical field
The detection of Chinese medicine preparation of the present invention, a kind of infrared spectrum discrimination method of paecilomyces hepiall chen specifically.
Background technology
Cordyceps sinensis, be that section ergot fungus cordyceps sinensis colonizes in stroma on the Hepialidae insect larvae and the complex of larva corpse, is a kind of traditional famous and precious tonic Chinese medicine material, and the multiple efficacies such as the function of immune system of adjusting, antitumor, antifatigue are arranged.In recent years, due to physical environment and exploit excessive impact, the output of Cordyceps sinensis reduces day by day, and needs that can't satisfying the market, hold at high price, so domestic, adopted with natural cs fungi fermentation method and obtained Cordycepts sinensis.Research shows, the main chemical compositions in fermentation cordyceps, clinical effect are all similar with Cordyceps sinensis, and wherein the content of the compositions such as amino acid, uridine, adenosine is all higher than Cordyceps sinensis.
The Cordyceps sinensis fungus that the bacterial classification that at present extracts, separates from natural cordyceps has been reported at present is nearly more than 300 kinds, in state-owned kind more than 60.The existing product about fermentation cordyceps of China has Jinshuibao, Ningxinbao Capsules, zhiling capsules, hundred to make capsule, Xinganbao Jrao nang.Different fermentations cordyceps product adopts the bacterial classification difference of fermentation, paecilomyces hepiall chen and tablet adopt Paecilomyces hepiali chen Cs-4 bacterium powder to make with extra care and form through liquid fermentation, hundred to make capsule adopt bacterial classification be that Cs-C-Q80 China is by a hair spore bacterium, it is that Cordycepts sinensis is that Gliocladiumroseum is through the artificial mycelium of cultivating gained that Xinganbao Jrao nang adopts bacterial classification, it is Clavicipitaceae fungi Chinese caterpillar fungus cephalo that Ningxinbao Capsules adopts bacterial classification, and it is the mould genus of spore fungi that zhiling capsules adopts bacterial classification.
Paecilomyces hepiall chen is the product that gone on the market, is filled and is obtained by fermentation cordyceps (Cs ?4), and its main pharmacodynamics composition is fermentation cordyceps (Cs ?4)
Current commercially available ferment cordyceps sinensis bacterium powder preparation is numerous, fermentation raw material used, fermented bacterium, fermentation condition, fermentation mode are all different, and existing quality standard is similar with method of quality control, be difficult to distinguish paecilomyces hepiall chen and other fermentation cordyceps and preparation thereof.
Summary of the invention
The object of the invention for provide a kind of easy to operate, expend less, the time is short, the method for free of contamination discriminating paecilomyces hepiall chen.This infrared spectrogram can be used as the paecilomyces hepiall chen quality control and with the method for the discriminating of like product.The present invention utilizes macroscopical fingerprint of infrared spectrum, by means of the variation characteristic of infrared spectrogram, directly or slightly doing the mathematics processing namely can directly be used in Quality Control, take full advantage of the difference of infrared spectrum technology Fast Monitoring Jinshuibao quality and discriminating and like product, have easy and simple to handle, quick, result is accurate and free of contamination advantage.
The present invention realizes by following scheme: a kind of infrared spectrum discrimination method of paecilomyces hepiall chen, and the method comprises the following steps:
A, get the paecilomyces hepiall chen content, 60～80 ℃ of drying 2～4h;
B, mix with potassium bromide dry paecilomyces hepiall chen content compressing tablet in the 1:100 ratio;
C, adopt the Fourier infrared spectrum analyzer to measure sample, and resolution is 4cm
-1, scanning times is 32 times, scanning wavelength is 400～4000cm
-1In scope;
D, adopt the OPUS analysis software to process infared spectrum, obtains dry paecilomyces hepiall chen content infrared spectrum;
E, contrast it, at 1400-1700cm with standard paecilomyces hepiall chen infrared spectrum
-1, 600-1200cm
-1Between the same characteristic features peak is arranged, be paecilomyces hepiall chen.
Wherein said standard paecilomyces hepiall chen characteristic peak spectrogram is for carrying out Fourier infrared spectrum mensuration with said method to the paecilomyces hepiall chen that conforms with the drug standards of at least 50 batches, and the paecilomyces hepiall chen characteristic peak obtained is 3295cm
-1, 2926cm
-1, 1650cm
-1, 1745cm
-1, 1239cm
-1, 1027cm
-1, 1745cm wherein
-1, 1239cm
-1, 1027cm
-1, be the peculiar peak of paecilomyces hepiall chen, and at 630cm
-1Place is without characteristic peak.Each batch characteristic peak wavelength is between difference ± 3.
Wherein, it being contrasted with standard paecilomyces hepiall chen characteristic peak spectrogram, is to adopt the second derivative method to process infared spectrum, by 1400-1700cm
-1, 600-1200cm
-1Correspondence is carried out in the peak position existed, and same or similar degree with interior, is paecilomyces hepiall chen 90%.
In wherein said method b, get dry paecilomyces hepiall chen content 0.8-1mg, add potassium bromide 80-100mg, the method that adopts equivalent to increase progressively progressively mixes.
With the cordyceps preparation of method of the present invention to other brands, detect, all at 1400-1700cm
-1, 600-1200cm
-1Peak position is without the peculiar peak of above-mentioned Jinshuibao.At this point, by method of the present invention, can detect and differentiate whether the product of buying on market is the genuine piece paecilomyces hepiall chen.
The invention has the advantages that: because infrared spectrum has the characteristics such as characteristic is strong, sampling amount is little, simple, convenient and rapid, and paecilomyces hepiall chen and like product complicated components, personal characteristics is not strong, its infrared spectrum is the wherein stack of a plurality of component infrared spectrums, the synergistic result of the absorption peak strength of spectrum and the identical or different functional group of peak form.In multiple batches of like product, various chemical compositions are relatively stable aspect quality and quantity, and sample treatment is consistent, and this method has certain objectivity and repeatability.Prove by experiment, the method is quick, stable, reliable, can be used as the method that the paecilomyces hepiall chen quality control reaches and like product is distinguished and uses.
The accompanying drawing explanation:
Fig. 1 paecilomyces hepiall chen and like product A infrared spectrogram;
Fig. 2 paecilomyces hepiall chen and like product A, C, D infrared spectrogram;
Fig. 3 paecilomyces hepiall chen and like product A, C, D infrared spectrogram second derivative are processed figure (wave-number range 1200-1800cm-1);
Fig. 4 paecilomyces hepiall chen and like product A, C, D infrared spectrogram second derivative are processed figure (wave-number range 600-1200cm
-1);
Embodiment:
Further illustrate by the following examples the present invention.
1 one kinds of paecilomyces hepiall chen infrared spectrum discrimination methods of embodiment
Get lot number and be 091001,090712,110153,100116,100706,090319,090303,090326,090327,090328 paecilomyces hepiall chen content, 60 ℃ are dried 4h.Get each lot number and dry content 1mg, add respectively KBr100mg, mix, compressing tablet, each lot number are got 10 samples, adopt the Fourier infrared spectrum analyzer to measure sample, and resolution is 4cm
-1, scanning times is 32 times, scanning wavelength is 400～4000cm
-1, and adopt the OPUS analysis software to process infared spectrum.In each batch sample, characteristic peak 3295cm is arranged all as a result
-1, 2926cm
-1, 1650cm
-1, 1745cm
-1, 1239cm
-1, 1027cm
-1, and at 630cm
-1Place is without characteristic peak.Show that this product is the paecilomyces hepiall chen certified products.
2 one kinds of paecilomyces hepiall chen infrared spectrum discrimination methods of embodiment
Get fermentation cordyceps commercially available prod A, B, C, D content, 60 ℃ are dried 4h.Get each product and dry content 1mg, add respectively KBr100mg, mix, compressing tablet, adopt the Fourier infrared spectrum analyzer to measure sample, and resolution is 4cm
-1, scanning times is 32 times, at 400～4000cm
-1In scope, carry out Infrared spectrum scanning, and adopt the OPUS analysis software to process infared spectrum.A, B, C, D infrared absorption peak all have 3295cm as a result
-1, 2926cm
-1, 1650cm
-1, exclusive 1745cm in the B sample
-1, 1239cm
-1, 1027cm
-1, and at 630cm
-1Place is without characteristic peak.Show that B is paecilomyces hepiall chen, A, C, D are like product.
Embodiment 3 paecilomyces hepiall chens
Prescription: fermentation cordyceps (Cs ?4) 330g
Method for making: get fermentation cordyceps (Cs ?4), be ground into fine powder, incapsulate, make 1000, obtain.
Claims (5)
1. the infrared spectrum discrimination method of a paecilomyces hepiall chen, it is characterized in that: the method comprises the following steps:
Step 1, get the paecilomyces hepiall chen content, 60～80 ℃ of drying 2～4h;
Step 2, mix with potassium bromide dry paecilomyces hepiall chen content compressing tablet in the 1:100 ratio;
Step 3, adopt the Fourier infrared spectrum analyzer to measure sample, and resolution is 4cm
-1, scanning times is 32 times, scanning wavelength is 400～4000cm
-1In scope;
Step 4, adopt the OPUS analysis software to process infared spectrum, obtains dry paecilomyces hepiall chen content infrared spectrum;
Step 5, contrast it, at 1400-1700cm with standard paecilomyces hepiall chen infrared spectrum
-1, 600-1200cm
-1Between the same characteristic features peak is arranged, be paecilomyces hepiall chen.
2. according to the discrimination method described in claim 1, it is characterized in that: wherein said standard paecilomyces hepiall chen characteristic peak spectrogram is for carrying out Fourier infrared spectrum mensuration with said method to the paecilomyces hepiall chen that conforms with the drug standards of at least 50 batches, and the paecilomyces hepiall chen characteristic peak obtained is 3295cm
-1, 2926cm
-1, 1650cm
-1, 1745cm
-1, 1239cm
-1, 1027cm
-1, 1745cm wherein
-1, 1239cm
-1, 1027cm
-1, be the peculiar peak of paecilomyces hepiall chen, and at 630cm
-1Place is without characteristic peak, and each batch characteristic peak wavelength is between difference ± 3.
3. according to the discrimination method described in claim 1, it is characterized in that: wherein, it being contrasted with standard paecilomyces hepiall chen characteristic peak spectrogram, is to adopt the second derivative method to process infared spectrum, by 1400-1700cm
-1, 600-1200cm
-1Correspondence is carried out in the peak position existed, and same or similar degree with interior, is paecilomyces hepiall chen 90%.
4. discrimination method according to claim 1 is characterized in that: in wherein said step 2, get dry paecilomyces hepiall chen content 0.8-1mg, add potassium bromide 80-100mg, the method that adopts equivalent to increase progressively progressively mixes.
5. discrimination method according to claim 1 is characterized in that: with the method for the claim 1 cordyceps preparation to other brands, detect, all at 1400-1700cm
-1, 600-1200cm
-1Peak position is without the peculiar peak of above-mentioned Jinshuibao.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103698296A (en) * | 2013-12-16 | 2014-04-02 | 张雪峰 | Infrared spectroscopic analysis and authentication method for cordyceps sinensis stromata |
| CN103760126A (en) * | 2013-12-16 | 2014-04-30 | 张雪峰 | Infrared spectroscopic analysis and identification method for polypide of Cordyceps sinensis |
| CN111337448A (en) * | 2020-03-26 | 2020-06-26 | 杭州老爸标准技术集团有限公司 | Method for identifying cosmetics based on infrared spectrum technology |
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| CN101118230A (en) * | 2007-07-16 | 2008-02-06 | 柯传奎 | Method for identifying cordyceps sinensis products and uses thereof |
| CN102768195A (en) * | 2012-06-29 | 2012-11-07 | 杭州中美华东制药有限公司 | Method for quickly detecting moisture content of cordyceps mycelia powder |
| CN103105374A (en) * | 2011-11-15 | 2013-05-15 | 天津天狮生物发展有限公司 | Method for quickly identifying true and false cordyceps mycelia |
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| CN101118230A (en) * | 2007-07-16 | 2008-02-06 | 柯传奎 | Method for identifying cordyceps sinensis products and uses thereof |
| CN103105374A (en) * | 2011-11-15 | 2013-05-15 | 天津天狮生物发展有限公司 | Method for quickly identifying true and false cordyceps mycelia |
| CN102768195A (en) * | 2012-06-29 | 2012-11-07 | 杭州中美华东制药有限公司 | Method for quickly detecting moisture content of cordyceps mycelia powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103698296A (en) * | 2013-12-16 | 2014-04-02 | 张雪峰 | Infrared spectroscopic analysis and authentication method for cordyceps sinensis stromata |
| CN103760126A (en) * | 2013-12-16 | 2014-04-30 | 张雪峰 | Infrared spectroscopic analysis and identification method for polypide of Cordyceps sinensis |
| CN103760126B (en) * | 2013-12-16 | 2016-01-27 | 青海春天药用资源科技利用有限公司 | The Infrared spectroscopy authentication method of Cordyceps sinensis polypide |
| CN103698296B (en) * | 2013-12-16 | 2016-06-01 | 青海春天药用资源科技利用有限公司 | The Infrared spectroscopy authentication method of Cordyceps sinensis stroma |
| CN111337448A (en) * | 2020-03-26 | 2020-06-26 | 杭州老爸标准技术集团有限公司 | Method for identifying cosmetics based on infrared spectrum technology |
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Application publication date: 20131127 |