CN101118230A - Method for identifying cordyceps sinensis products and uses thereof - Google Patents
Method for identifying cordyceps sinensis products and uses thereof Download PDFInfo
- Publication number
- CN101118230A CN101118230A CNA200710070009XA CN200710070009A CN101118230A CN 101118230 A CN101118230 A CN 101118230A CN A200710070009X A CNA200710070009X A CN A200710070009XA CN 200710070009 A CN200710070009 A CN 200710070009A CN 101118230 A CN101118230 A CN 101118230A
- Authority
- CN
- China
- Prior art keywords
- cordyceps
- cordyceps sinensis
- products
- product
- sinensis products
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention provides a cordyceps product verifying method and application. The analysis on amylase and monosaccharose composition of different cordyceps products shows great difference; thereby verifying the types of cordyceps by analyzing the amylase and monosaccharose constitutes of cordyceps products. The present invention is suitable for natural cordyceps, artificial fermentation cordyceps and artificial fake cordyceps. As for a certain cordyceps product, the analysis of the amylase and monosaccharose composition is carried on the samples from the different batch produced or different production regions, and the result shows no remarkable difference, therefore, the method provided by the present invention can be used to verify batch stability of cordyceps products to control the quality of products.
Description
(1) technical field
The present invention relates to a kind of discrimination method and application thereof of cordyceps sinensis products.
(2) background technology
The Chinese caterpillar fungus that this case is narrated refers to the worm grass product of the natural cs of Cordyceps, artificial pseudo-Chinese caterpillar fungus or Cordyceps strain fermentation.
Cordyceps has kind more than 300 according to incompletely statistics, can retrieve nearly 497 kinds on the fungus name database that the CABI Bioscience of Britain provides, but some are arranged is different names of the same race.Be distributed at present China greatly about about 70 kinds.Wherein foremost, common people generally are familiar with is Cordyceps sinensis, latin name is Cordyceps sinensis (Berk.) Sacc..The Chinese caterpillar fungus of putting down in writing in the ancient medical skill of China generally also is meant Cordyceps sinensis.In addition, common also have Cordyceps militaris, Cordyceps hawkesii Gary, Cordyceps gunnii (Berk.) Berk or the like.Except common in the market some the artificial cordyceps products in addition of natural cs, these worm grass products mostly are to separate to obtain bacterial classification from a certain natural cordyceps militaris sporocarp of Cordyceps, then adopt the liquid or solid ARTIFICIAL CULTURE, obtain a large amount of mycelium or fructification.Just be to use such as the raw material of Jinshuibao and separate the mycelium that the peacilomyce hepiahi bacterium kind liquid fermentation and culture that obtains obtains, hundred to make the raw material of capsule be the mycelium that Hirsutella hepiali Chen et Shen kind liquid fermentation and culture obtains, and these two kinds are all recorded by 2005 editions " Pharmacopoeia of People's Republic of China (one one) ".Obtain product by strain fermentation and also have Ning Xinbao, zhiling capsules and caterpillar fungus cephalosporin capsule etc., also have miscellaneous various Chinese caterpillar fungus class health products, have plenty of by strain fermentation and form, have plenty of solid fermentation and cultivate and form as northern Chinese caterpillar fungus.Even some are artificial pseudo-product in addition, have plenty of by the flour pressing mold to form, and have plenty of the puppet product of making of day lily, and are multifarious.
In the face of miscellaneous worm grass product on the market, natural, the artificial fermentation cultivates, artificial pseudo-product, the judging standard that neither one is unified.Mainly differentiate at present for natural cs, but this needs suitable experience, be not easy to grasp by outward appearance.Mycelium product to fermented and cultured is difficult to differentiate by outward appearance, generally the ucleosides composition is differentiated, makes capsule as hundred.The effective constituent of the Chinese caterpillar fungus class of bibliographical information is a lot, and nucleosides is one of them, and content is very low, makes in the capsule adenosine content only less than per mille such as hundred.Wherein also have a lot of content higher effective compositions not have good method of quality control, such as polysaccharide.Iff polyoses content being measured and not representative,, all contain polysaccharide in a lot of materials because polysaccharide is widely distributed.The invention provides a kind of method, by being formed, the monose of the polysaccharide of various worm grass products measures, the monose composition of finding polysaccharide in the various worm grass products has marked difference, and it is very little to form difference with the monose of the different places of production of a kind of worm grass product or batch polysaccharide, so can be used as the different worm grass product of judging standard difference; The monose of the polysaccharide of the same fermented product of different batches is formed basically identical, and reliable standard can be provided the quality control with a kind of worm grass product.Can better control the quality of cordyceps sinensis products as the replenishing of methods such as discrimination method at present commonly used such as outward appearance, ucleosides composition, amino acid discriminating.
(3) summary of the invention
The invention provides discrimination method of a kind of cordyceps sinensis products and uses thereof, the monose of the polysaccharide by measuring worm grass product is formed, the result is listed as into the table of comparisons, the monose of the polysaccharide of cordyceps sinensis products to be measured is formed with table of comparisons contrast judged the kind of product to be measured and true and false.Detailed process is as follows:
It is an amount of to take by weighing cordyceps sinensis products, adds the water refluxing extraction 2 hours of 8 times of weight, and extract obtains supernatant through centrifuging, and supernatant adds the absolute ethyl alcohol precipitation of 4 times of volumes, leaves standstill to separate after 2 hours to obtain precipitation, and the precipitation drying obtains the Chinese caterpillar fungus crude polysaccharides.Get about 0.02 gram of crude polysaccharides, add the H of 2mol/L
2SO
4Solution 2ml, sealing, the mixing dissolving in 125 ℃ of reactions 1 hour, is put to room temperature, centrifugal 10 minutes of 3500r/min, supernatant is transferred pH to 7.0 with the NaOH aqueous solution of 4mol/L, measures this hydrolyzate 1.0ml in the hydrolysis pipe, adds each 1.0ml of methanol solution of 0.3mol/L NaOH aqueous solution and 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone, mixing, sealing is reacted 30min in 70 ℃ of water-baths, take out to put to be chilled to room temperature, transfer pH to 7.0 with hydrochloric acid, add the 2ml chloroform extraction, 3 times repeatedly, water layer 0.45um membrane filtration mistake, filtrate behind the mistake film is injected the liquid chromatograph analysis, and contrast standard product chromatogram is judged the kind and the ratio of monose; Liquid-phase condition is: UV-detector detects wavelength 250nm, the SB-C8 reverse-phase chromatographic column, and moving phase is that phosphate buffer and the second eyeball of pH5.8 formed flow velocity 1ml/min with volume ratio at 80: 20.Collect all cordyceps sinensis products on the market, survey the monose of its polysaccharide respectively and form, the result is listed as into the table of comparisons, the monose of the polysaccharide of cordyceps sinensis products to be measured is formed with table of comparisons contrast judged the kind of product to be measured and true and false.
This method one of is used and to be to form that there were significant differences and can judge the kind of Chinese caterpillar fungus and true and false according to the monose of different natural cs product polysaccharide, distinguishes natural cs product not of the same race.
Two of the application of this method is for a certain worm grass product, by the monose composition measuring to the polysaccharide of product between its different batches, judges the stability of product, as one of index of production quality control.
Three of the application of this method is between the Chinese caterpillar fungus fermentation series products because the difference of bacterial classification, production technology, bacterial strain, and the monose of its product polysaccharide forms that there were significant differences, so can be used for discriminating.
The zymotechnique of the bacterial classification of the places of origin of raw materials of worm grass product, the kind of natural cs, ferment cordyceps sinensis mycelium and bacterial strain, ferment cordyceps sinensis mycelium etc.These factors all may cause the monose of its polysaccharide to form difference.Natural cordyceps itself is that the complex of worm and grass is used as medicine jointly, so also be wholely to pulverize that extract the back and the monose composition of mensuration polysaccharide when detecting with this law; Jinshuibao and hundred makes the raw material of capsule belong to the mycelium that the bacterial classification liquid fermentation produces, so make the content of capsule extract polysaccharide and mensuration composition to Jinshuibao and hundred.The mycelium product that the different strains fermentation of same bacterial classification obtains, the monose composition of its polysaccharide also is not quite similar, making the bacterial classification of the Hirsutella hepiali Chen et Shen filament powder of capsule and Zhejiang Cifu Pharmaceutical Co., Ltd.'s production such as hundred of East China medicine company limited production all is Hirsutella hepiali Chen et Shen, but bacterial strain difference, technology is also variant slightly, the monose of the polysaccharide of both products is formed obvious difference as a result, so the resolution of this assay method is still than higher.If measure with the DNA method, then the DNA measurement result of the same bacterial classification of different strains must be consistent, can't distinguish.So this authentication method can be used as one of standard of worm grass product quality control.And for same worm grass product, measure the monose of the polysaccharide of each batch products forms by this method, can differentiate the difference of different batches product, if come to the same thing and show constant product quality, if there were significant differences shows that then product quality is stable inadequately for the result.Compare in this and use authoritative at present DNA authentication technique that outstanding advantage is arranged, dna technique can only carry out difference to bacterial classification, the DNA difference of different strain, there were significant differences such as the DNA of Cordyceps militaris and Cordyceps sinensis, but then can't determine the stability of product for the product of same bacterial classification different batches.And this method has not only reflected the difference of different strain to the monose composition measuring of polysaccharide, but also whether reflected whole fermentation process stable.Because it is it is not only relevant with bacterial classification that the monose of polysaccharide is formed, also relevant with whole process of production.But the effect of this method neither be absolute, can not think that this method can replace the methods such as physicochemical identification of DNA discriminating and other non-saccharic compositions.Complement each other between these methods, each have their own advantage, this method are that one of other method is replenished.
For natural worm grass product, the monose of the worm grass product polysaccharide of different strain is formed significant difference, but the different places of production of the product of same bacterial classification then the monose of polysaccharide to form difference not remarkable.All can cause the monose of the polysaccharide of product to form significant difference for fermented and cultured product different strain, with bacterial classification different strains etc.Then relevant for artificial pseudo-Chinese caterpillar fungus with the starting material of making pseudo-Chinese caterpillar fungus.
(4) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
Take by weighing each about 1 gram of natural cordyceps, Cordyceps militaris, Cordyceps hawkesii Gary and artificial pseudo-Chinese caterpillar fungus, the water refluxing extraction 2 hours that adds 10 times of weight, extract obtains supernatant through centrifuging, supernatant adds the absolute ethyl alcohol precipitation of 4 times of volumes, leave standstill after 2 hours to separate and obtain precipitation, the precipitation drying obtains the Chinese caterpillar fungus crude polysaccharides.Get about 0.02 gram of crude polysaccharides, add the H of 2mol/L
2SO
4Solution 2ml, sealing, the mixing dissolving in 125 ℃ of reactions 1 hour, is put to room temperature, centrifugal 10 minutes of 3500r/min, supernatant is transferred pH to 7.0 with the NaOH aqueous solution of 4mol/L, measures this hydrolyzate 1.0ml in the hydrolysis pipe, adds each 1.0ml of methanol solution of 0.3mol/L NaOH aqueous solution and 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone, mixing, sealing is reacted 30min in 70 ℃ of water-baths, take out to put to be chilled to room temperature, transfer pH to 7.0 with hydrochloric acid, add the 2ml chloroform extraction, 3 times repeatedly, water layer 0.45um membrane filtration mistake, filtrate behind the mistake film is injected the liquid chromatograph analysis, and contrast standard product chromatogram is judged the kind and the ratio of monose; Liquid-phase condition is: UV-detector detects wavelength 250nm, the SB-C8 reverse-phase chromatographic column, and moving phase is that phosphate buffer and the second eyeball of pH5.8 formed flow velocity 1ml/min with volume ratio at 80: 20.Result such as following table 1:
Table 1
Embodiment 2:
Take by weighing each about 1 gram of natural cordyceps from Qinghai, Sichuan, Yunnan, Tibet and Gansu, the water refluxing extraction 2 hours that adds 8 times of weight, extract obtains supernatant through centrifuging, supernatant adds the absolute ethyl alcohol precipitation of 4 times of volumes, leave standstill after 2 hours to separate and obtain precipitation, the precipitation drying obtains the Chinese caterpillar fungus crude polysaccharides.Get the about 0.02g of crude polysaccharides, add the trifluoroacetic acid 2ml of 2M, the sealing mixing, reaction is 1 hour in 125 ℃ of baking ovens, puts to room temperature, flows down at nitrogen and dries up hydrolyzate.Add 1ml methyl alcohol, flow down at nitrogen behind the mixing and dry up.Sample after drying up adds distilled water 1ml, sodium borohydride powder 25mg, mixing reaction 2 hours.Splash into the acetic acid stopped reaction till do not have a bubble.Flow down at nitrogen and to dry up reactant liquor, add hydrochloric acid-methyl alcohol 2ml of 0.1% (V/V), mixing, nitrogen dries up, and repeats 3 times.105 ℃ of baking oven for heating of residue were removed moisture in 1 hour.Add 0.5ml pyridine and 0.5ml acetic anhydride, reacted 1 hour in boiling water bath behind the tube sealing, reaction product is carried out gas chromatographic analysis monose and is formed.(2m * 0.3cm), injector and detecting device (FID) are 250 ℃ to the 1%0V-225 packed column; Column temperature: 195 ℃; H230mL/min; Air 200mL/min; Carrier gas is high-purity N 2:10mL/min; Sample size 0.6 μ L.The results are shown in following table 2:
Table 2
Embodiment 3:
Take by weighing paecilomyces hepiall chen content, hundred and make capsule 's content, peaceful heart health-care capsule content and Zhejiang Cifu Pharmaceutical Co., Ltd. produce each about 1 gram of Hirsutella hepiali Chen et Shen filament powder, add 15 milliliters in water, 80 degree ultrasonic Extraction 2 hours.Extract obtains supernatant through centrifuging, and supernatant adds the absolute ethyl alcohol precipitation of 4 times of volumes, leaves standstill to separate after 2 hours to obtain precipitation, and the precipitation drying obtains the Chinese caterpillar fungus crude polysaccharides.Get about 0.02 gram of crude polysaccharides, add the H of 2mol/L
2SO
4Solution 2ml, sealing, the mixing dissolving in 125 ℃ of reactions 1 hour, is put to room temperature, centrifugal 10 minutes of 3500r/min, supernatant is transferred pH to 7.0 with the NaOH aqueous solution of 4mol/L, measures this hydrolyzate 1.0ml in the hydrolysis pipe, adds each 1.0ml of methanol solution of 0.3mol/L NaOH aqueous solution and 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone, mixing, sealing is reacted 30min in 70 ℃ of water-baths, take out to put to be chilled to room temperature, transfer pH to 7.0 with hydrochloric acid, add the 2ml chloroform extraction, 3 times repeatedly, water layer 0.45um membrane filtration mistake, filtrate behind the mistake film is injected the liquid chromatograph analysis, and contrast standard product chromatogram is judged the kind and the ratio of monose; Liquid-phase condition is: UV-detector detects wavelength 250nm, the SB-C8 reverse-phase chromatographic column, and moving phase is that phosphate buffer and the second eyeball of pH5.8 formed flow velocity 1ml/min with volume ratio at 80: 20.
Result such as following table 3:
Table 3
Embodiment 4:
Take by weighing each about 1 gram of Hirsutella hepiali Chen et Shen filament powder of four different production batch of Zhejiang Cifu Pharmaceutical Co., Ltd.'s product, add 15 milliliters in water, 80 degree ultrasonic Extraction 2 hours.Extract obtains supernatant through centrifuging, and supernatant adds the absolute ethyl alcohol precipitation of 4 times of volumes, leaves standstill to separate after 2 hours to obtain precipitation, and the precipitation drying obtains the Chinese caterpillar fungus crude polysaccharides.Get about 0.02 gram of crude polysaccharides, the trifluoroacetic acid solution 2ml that adds 2mol/L, sealing, the mixing dissolving, in 125 ℃ of reactions 1 hour, put to room temperature, centrifugal 10 minutes of 3500r/min, supernatant is transferred pH to 7.0 with the NaOH aqueous solution of 4mol/L, measure this hydrolyzate 1.0ml in the hydrolysis pipe, each 1.0ml of methanol solution that adds 0.3mol/L NaOH aqueous solution and 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone, mixing, sealing, in 70 ℃ of water-baths, react 30min, taking-up is put and is chilled to room temperature, transfers pH to 7.0 with hydrochloric acid, adds the 2ml chloroform extraction, 3 times repeatedly, water layer usefulness 0.45um membrane filtration mistake is injected the liquid chromatograph analysis with the filtrate behind the mistake film, and contrast standard product chromatogram is judged the kind and the ratio of monose; Liquid-phase condition is: UV-detector detects wavelength 250nm, the SB-C8 reverse-phase chromatographic column, and moving phase is that phosphate buffer and the second eyeball of pH5.8 formed flow velocity 1ml/min with volume ratio at 80: 20.Result such as following table 4:
Table 4
Claims (7)
1. the discrimination method of a cordyceps sinensis products, it is characterized in that: it is an amount of to take by weighing cordyceps sinensis products, the water refluxing extraction 2 hours that adds 8 times of weight, extract obtains supernatant through centrifuging, supernatant adds the absolute ethyl alcohol precipitation of 4 times of volumes, leave standstill after 2 hours to separate and obtain precipitation, the precipitation drying obtains the Chinese caterpillar fungus crude polysaccharides.Get about 0.02 gram of crude polysaccharides, add the H of 2mol/L
2SO
4Solution 2ml, sealing, the mixing dissolving in 125 ℃ of reactions 1 hour, is put to room temperature, centrifugal 10 minutes of 3500r/min, supernatant is transferred pH to 7.0 with the NaOH aqueous solution of 4mol/L, measures this hydrolyzate 1.0ml in the hydrolysis pipe, adds each 1.0ml of methanol solution of 0.3mol/L NaOH aqueous solution and 0.5mol/L 1-phenyl-3-methyl-5-pyrazolones ketone, mixing, sealing is reacted 30min in 70 ℃ of water-baths, take out to put to be chilled to room temperature, transfer pH to 7.0 with hydrochloric acid, add the 2ml chloroform extraction, 3 times repeatedly, water layer 0.45um membrane filtration mistake, filtrate behind the mistake film is injected the liquid chromatograph analysis, and contrast standard product chromatogram is judged the kind and the ratio of monose; Liquid-phase condition is: UV-detector detects wavelength 250nm, the SB-C8 reverse-phase chromatographic column, and moving phase is that phosphate buffer and the second eyeball of pH5.8 formed flow velocity 1ml/min with volume ratio at 80: 20; Collect all cordyceps sinensis products on the market, survey the monose of its polysaccharide respectively and form, the result is listed as into the table of comparisons, the monose of the polysaccharide of cordyceps sinensis products to be measured is formed with table of comparisons contrast judged the kind of product to be measured and true and false.
2. the discrimination method of a kind of cordyceps sinensis products as claimed in claim 1, it is characterized in that: the extraction of cordyceps sinensis products crude polysaccharides can be adopted ultrasonic Extraction, also can adopt refluxing extraction, Soxhlet to extract and method such as dipping extraction.
3. the discrimination method of a kind of cordyceps sinensis products as claimed in claim 1, it is characterized in that: the hydrolysis of crude polysaccharides can be selected organic acid such as trifluoroacetic acid, trichloroacetic acid etc., also can select mineral acid such as sulfuric acid, hydrochloric acid etc., also can select enzyme reagent as mixing SNSP hydrolytic enzyme, complex enzyme and other enzyme.
4. the discrimination method of a kind of cordyceps sinensis products as claimed in claim 1 is characterized in that: measure the method that the monose of crude polysaccharides forms and can adopt high-efficient liquid phase technique also can adopt methods such as vapor-phase chromatography, thin-layered chromatography, capillary electrophoresis.
5. the discrimination method of a kind of cordyceps sinensis products as claimed in claim 1, it is characterized in that: cordyceps sinensis products comprises that fermentation class worm grass product makes capsule, Hirsutella hepiali Chen et Shen fermentate, Jinshuibao, Ning Xinbao and fermentation Cordyceps militaris etc. as hundred, also comprise natural cs series products such as cordyceps sporophore, Cordyceps hawkesii Gary fructification, fruiting bodies of cordyceps militaris etc., comprise that also made on the market pseudo-cordyceps sinensis products is as product of forming with pressing molds such as starch etc.
6. as the application of the discrimination method of the described a kind of cordyceps sinensis products of claim 1～5, it is characterized in that: the method can be used for differentiating the kind of fermentation class Chinese caterpillar fungus, natural cs and artificial pseudo-Chinese caterpillar fungus class and true and false.
7. as the application of the discrimination method of the described a kind of cordyceps sinensis products of claim 1～5, it is characterized in that: the method can be used as the product quality that quality index is controlled a certain cordyceps sinensis products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710070009XA CN101118230A (en) | 2007-07-16 | 2007-07-16 | Method for identifying cordyceps sinensis products and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710070009XA CN101118230A (en) | 2007-07-16 | 2007-07-16 | Method for identifying cordyceps sinensis products and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101118230A true CN101118230A (en) | 2008-02-06 |
Family
ID=39054429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710070009XA Pending CN101118230A (en) | 2007-07-16 | 2007-07-16 | Method for identifying cordyceps sinensis products and uses thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101118230A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102589956A (en) * | 2010-04-21 | 2012-07-18 | 张雪峰 | Method for identifying cordyceps sinensis through microscopic staining |
| CN102690797A (en) * | 2012-05-28 | 2012-09-26 | 浙江工业大学 | Enzyme for synthesizing and metabolizing adenine of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis, and gene and application of enzyme |
| CN102747057A (en) * | 2012-05-28 | 2012-10-24 | 浙江工业大学 | Cordyceps sinensis hirsutella sinensis purine anabolism enzyme, gene thereof, and application thereof |
| CN103411915A (en) * | 2013-07-26 | 2013-11-27 | 江西济民可信金水宝制药有限公司 | Infrared spectroscopy identification method of traditional Chinese medicine preparation-Jinshuibao capsule |
| CN104597195A (en) * | 2013-10-30 | 2015-05-06 | 宜昌山城水都冬虫夏草有限公司 | Method for discriminating cordyceps sinensis storage time |
| CN108535372A (en) * | 2018-03-21 | 2018-09-14 | 江西国药有限责任公司 | The construction method and its finger-print of fermentation cordyceps and preparation sterols HPLC finger-prints |
| CN109164175A (en) * | 2018-07-04 | 2019-01-08 | 中国农业科学院北京畜牧兽医研究所 | A kind of feed non-starch polysaccharide component analysis method |
-
2007
- 2007-07-16 CN CNA200710070009XA patent/CN101118230A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102589956A (en) * | 2010-04-21 | 2012-07-18 | 张雪峰 | Method for identifying cordyceps sinensis through microscopic staining |
| CN102589956B (en) * | 2010-04-21 | 2014-07-09 | 青海春天药用资源科技利用有限公司 | Method for identifying cordyceps sinensis through microscopic staining |
| CN102690797A (en) * | 2012-05-28 | 2012-09-26 | 浙江工业大学 | Enzyme for synthesizing and metabolizing adenine of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis, and gene and application of enzyme |
| CN102747057A (en) * | 2012-05-28 | 2012-10-24 | 浙江工业大学 | Cordyceps sinensis hirsutella sinensis purine anabolism enzyme, gene thereof, and application thereof |
| CN102747057B (en) * | 2012-05-28 | 2015-04-22 | 浙江工业大学 | Cordyceps sinensis hirsutella sinensis purine anabolism enzyme, gene thereof, and application thereof |
| CN103411915A (en) * | 2013-07-26 | 2013-11-27 | 江西济民可信金水宝制药有限公司 | Infrared spectroscopy identification method of traditional Chinese medicine preparation-Jinshuibao capsule |
| CN104597195A (en) * | 2013-10-30 | 2015-05-06 | 宜昌山城水都冬虫夏草有限公司 | Method for discriminating cordyceps sinensis storage time |
| CN104597195B (en) * | 2013-10-30 | 2017-03-15 | 宜昌山城水都冬虫夏草有限公司 | A kind of method for differentiating Cordyceps storage time |
| CN108535372A (en) * | 2018-03-21 | 2018-09-14 | 江西国药有限责任公司 | The construction method and its finger-print of fermentation cordyceps and preparation sterols HPLC finger-prints |
| CN108535372B (en) * | 2018-03-21 | 2020-10-09 | 江西国药有限责任公司 | Fermented cordyceps sinensis powder and preparation sterol HPLC fingerprint construction method and fingerprint thereof |
| CN109164175A (en) * | 2018-07-04 | 2019-01-08 | 中国农业科学院北京畜牧兽医研究所 | A kind of feed non-starch polysaccharide component analysis method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101118230A (en) | Method for identifying cordyceps sinensis products and uses thereof | |
| CN101177665B (en) | Industrial method for culturing north winter worm summer herb with high-content of cordycepin | |
| CN101955549B (en) | Large-headed atractylodes rhizome polysaccharide and preparation method and application thereof | |
| CN102242069B (en) | Paecilomycescicadae (Miq.)Samson and application thereof | |
| CN102220270A (en) | Screening method for producing chondroitin sulfate bacterial strain and application of bacterial strain fermentation method in production of chondroitin sulfate | |
| CN104013657A (en) | Post-fermentation extracting method of extracting saponin from American ginseng medicinal material | |
| CN110438180B (en) | Preparation of ganoderma lucidum liquid fermentation extracellular active polysaccharide and application thereof in enhancing immunity | |
| CN104945527A (en) | Galactomannan antigen and preparation method thereof | |
| CN109651532A (en) | A kind of dendrobium candidum Glucomannan | |
| CN109400741A (en) | A kind of isolation and purification method of ganoderma spore polysaccharide | |
| CN101914586B (en) | Method for preparing and purifying DON toxin | |
| CN105153321A (en) | Quick separation method of lotus seed oligosaccharide monomers with prebiotic effect | |
| CN113564069A (en) | Bifidobacterium longum and bifidobacterium longum exopolysaccharide as well as extraction method and application thereof | |
| CN106990153A (en) | A kind of method for differentiating astragalus mongolicus and Astragalus membranacus | |
| CN105147719B (en) | A kind of preparation of Yupingfeng dregs of a decoction polysaccharide symphysis unit and its application in immunological regulation | |
| CN103993051A (en) | Method for preparing water-soluble active polysaccharide in Calvatia candida mycelia | |
| CN104535513A (en) | Glabrous sarcandra herb extract and detection method of preparation thereof | |
| CN105779338B (en) | A kind of ice cream butter oil substitute and method using the preparation of streptococcus thermophilus fermentation whey | |
| CN102898537A (en) | Purification method of lipopolysaccharide | |
| CN113604522A (en) | Penicillium D306 strain capable of producing extracellular polysaccharide and application thereof in preparation of bile acid binder | |
| CN106754562A (en) | A kind of method that the bacterial strain and its fermented tcm discarded object of high yield bacterial polysaccharides prepare polysaccharide | |
| CN101109007A (en) | Quality control method for cryptoporus volvatus hubb. zymolysis technique | |
| CN102094053B (en) | Method for purifying streptococcus penumoniae C polysaccharide | |
| CN102321620B (en) | Molecular standard sample for bull's eye rot bacteria on apples and preparation method thereof | |
| CN112094751B (en) | Fermentation liquor of spirillum obliquus QYL-10, biological additive produced by fermentation liquor and application of fermentation liquor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080206 |