CN103760126A - Infrared spectroscopic analysis and identification method for polypide of Cordyceps sinensis - Google Patents
Infrared spectroscopic analysis and identification method for polypide of Cordyceps sinensis Download PDFInfo
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- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 122
- 238000000034 method Methods 0.000 title claims abstract description 47
- 238000012844 infrared spectroscopy analysis Methods 0.000 title abstract 2
- 238000001228 spectrum Methods 0.000 claims abstract description 72
- 238000002329 infrared spectrum Methods 0.000 claims description 97
- 238000012360 testing method Methods 0.000 claims description 20
- 238000002835 absorbance Methods 0.000 claims description 15
- 238000001965 diffuse reflectance infrared spectroscopy Methods 0.000 claims description 13
- 238000004566 IR spectroscopy Methods 0.000 claims description 12
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Abstract
The invention relates to the field of analytical chemistry, specifically to an infrared spectroscopic analysis and identification method for the polypide of Cordyceps sinensis. The objective of the invention is to overcome the technical problem that whether the polypide of Cordyceps sinensis is true or false is difficult to determine at present. Thus, the invention provides the method for rapid and accurate discrimination of the polypide of Cordyceps sinensis. The method comprises the following steps: a, drafting the standard spectrum of the polypide of Cordyceps sinensis; b, acquiring necessary infrared spectroscopic characteristic peaks and common infrared spectroscopic characteristic peaks of the polypide of Cordyceps sinensis; and c, comparing infrared spectroscopic characteristic peaks and infrared spectroscopic similarity of a detected sample of the polypide of Cordyceps sinensis. The invention provides the novel method for rapid and accurate discrimination of the polypide of Cordyceps sinensis.
Description
Technical field
The present invention relates to analytical chemistry field.Specifically, the Infrared spectroscopy authentication method that relates to Cordyceps sinensis polypide.
Background technology
Cordyceps sinensis is that section ergot fungus cordyceps sinensis bacterium (Cordyceps sinensis (Berk.) Sacc.) colonizes in stroma on Hepialidae insect bat moth larvae and the complex of larva corpse, the effect with kidney tonifying benefit lung, hemostasis and phlegm, with ginseng, pilose antler and be called China's three large tonics.
Cordyceps sinensis growing environment is special, is mainly distributed in mesophorbium, coryphile and the alpine scrub of China's Qinghai-Tibet Platean height above sea level 3000～5000m.Qinghai, Tibet, Sichuan, Gansu and Yunnan are the major production areas of Cordyceps sinensis, and the output in Qinghai and quality occupy first of each provinces and regions, and cajaput, Golog etc. is Cordyceps of Qinghai Province main product ground.Cordyceps sinensis still can not carry out artificial culture at present, and the distributed areas of wild cordyceps are narrow and small, and natural parasitic rate is low, harsh to requirement for environmental conditions, in addition ecological disruption and predation formula in recent years excavated, and the output of Cordyceps sinensis is declined year by year, and price is constantly soaring.Therefore, on market, there is the serious fakement phenomenas such as puppet, weightening finish of mixing.Except Cordyceps sinensis, other Cordyceps sinensis fungus colonize in the complex forming on insect bodies and are also referred to as " Chinese caterpillar fungus ", and some of them are often used as the adulterant of Cordyceps sinensis, and proterties is similar and be difficult to distinguish.Therefore set up Cordyceps sinensis polypide true and false product discrimination method fast and accurately, to standard market, guarantee that quality of medicinal material has very important significance.
Summary of the invention
To be solved by this invention is the technical matters that current Cordyceps sinensis polypide is difficult to minute true and false.
The technical scheme that the present invention solves the problems of the technologies described above is to provide a kind of method of quick and precisely differentiating Cordyceps sinensis polypide.The method comprises the following steps:
A, Cordyceps sinensis polypide standard spectrum are drawn: the standard Cordyceps sinensis polypide sample in a plurality of different places of production is pressed into respectively thin slice and carries out transmission test, and the averaged spectrum of all samples is Cordyceps sinensis polypide standard spectrum;
The collection of b, the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum and the common characteristic peak of Cordyceps sinensis polypide infrared spectrum;
The diffuse reflectance infrared spectroscopy peak comparison of c, tested Cordyceps sinensis polypide sample and the comparison of infrared spectrum similarity: in the infrared spectrum of sample, comprise the indispensable characteristic peak of whole Cordyceps sinensis polypide infrared spectrums and the more than 60% of the common characteristic peak of Cordyceps sinensis polypide infrared spectrum, and the related coefficient between sample spectrum and Cordyceps sinensis polypide standard spectrum is that level of significance is that the related coefficient lower threshold of 0.01 o'clock is 0.95, and this sample is genuine piece Cordyceps sinensis polypide.
Wherein, in the step a of the Infrared spectroscopy authentication method of above-mentioned Cordyceps sinensis polypide, described Cordyceps sinensis polypide sample is to be selected from Yushu district, Qinghai state, national main producing region, Forage Land In Guoluo Prefecture, state, Hainan and Tibet, Sichuan and area, 6, Gansu, and each provenance selection is not less than 8 uniform samples.
Wherein, in the step a of the Infrared spectroscopy authentication method of above-mentioned Cordyceps sinensis polypide, described Cordyceps sinensis polypide sample is by the natural drying rear pulverizing of the polypide part of Cordyceps sinensis, gets fine powder after 200 mesh sieves for examination of infrared spectrum.
Wherein, in the step a of said method, the standard Cordyceps sinensis polypide sample in a plurality of different places of production is pressed into respectively thin slice and carries out transmission when test, carries out under the same conditions compressing tablet test, as spectral background with the pure potassium bromide that does not add any sample.
Wherein, in the step a of said method, the transmitance maximal value of every sample infrared spectrum and minimum value differ and should be not less than 60%.
Wherein, in the step a of said method, each sample replication 3～5 times, gets its averaged spectrum as the spectrum of this sample.
Wherein, in the step a of said method, Infrared spectrum scanning scope is 4000～400cm
-1, spectral resolution is 4cm
-1, every spectrum cumulative scan 16 times or 32 times, deducts in real time carbon dioxide and water vapor and disturbs.
Wherein, in the step b of said method, the indispensable characteristic peak of described Cordyceps sinensis polypide infrared spectrum refers to the infrared spectrum absorpting peak position of comparison all samples, the characteristic peak that the absorption peak all occurring in all samples must be had as Cordyceps sinensis polypide infrared spectrum.The indispensable characteristic peak of described Cordyceps sinensis polypide infrared spectrum is as shown in table 1:
The indispensable characteristic peak of table 1 Cordyceps sinensis herb infrared spectrum
| Peak numbering | Peak position mean value (cm -1） | Deviation (cm in peak position under 99% degree of confidence -1） |
| A01 | 3292 | 11.4 |
| A02 | 2926 | 2.3 |
| A03 | 1657 | 2.8 |
| A04 | 1546 | 3.9 |
| A05 | 1460 | 8.5 |
| A06 | 1377 | 1.3 |
| A07 | 1249 | 8.5 |
| A08 | 1154 | 5.2 |
| A09 | 1083 | 2.8 |
| A10 | 1024 | 3.4 |
| A11 | 930 | 2.1 |
| A12 | 888 | 4.6 |
| A13 | 625 | 5.9 |
| A14 | 416 | 4.4 |
Wherein, in the step b of said method, the described common characteristic peak of Cordyceps sinensis polypide infrared spectrum refers in the infrared spectrum absorpting peak of all samples, except indispensable characteristic peak, probability of occurrence is not less than to 90% absorption peak, as the common characteristic peak of Cordyceps sinensis polypide infrared spectrum.The common characteristic peak of described Cordyceps sinensis polypide infrared spectrum is as shown in table 2:
The common characteristic peak of table 2 Cordyceps sinensis herb infrared spectrum
| Peak numbering | Peak position mean value (cm -1） | Deviation (cm in peak position under 99% degree of confidence -1） |
| B01 | 2854 | 1.3 |
| B02 | 1745 | 2.1 |
| B03 | 716 | 11.1 |
| B04 | 575 | 4.4 |
Wherein, in the step c of said method, described related coefficient is calculated by following formula:
In formula 1, R
ijrepresent the related coefficient of infrared spectrum i and infrared spectrum j, x
ikand x
jkrepresentative sample i and sample j are at the absorbance at wave number k place respectively.
The present invention uses AssureID software to calculate 1800～800cm
-1in scope, the related coefficient of each sample infrared spectrum and standard spectrum, adds up the distributed area of the related coefficient of each sample infrared spectrum and standard spectrum, determines that level of significance is the related coefficient lower threshold of 0.01 o'clock.
The present invention chooses the typical sample in the different places of production, national main producing region, has determined the common characteristic peak of Cordyceps sinensis polypide, has set up Cordyceps sinensis polypide standard infrared spectrum, and has determined the correlation coefficient threshold of genuine piece and standard spectrum.Integrated using diffuse reflectance infrared spectroscopy peak differential method and similarity differential method, can be objective, accurate, easy, rapidly Cordyceps sinensis polypide is differentiated.
Infrared spectrum differentiates for the true and false of various Chinese crude drugs, have objective quantification, result accurate, easy and simple to handle, test the plurality of advantages such as rapid, with low cost.The present invention utilizes infra-red sepectrometry to set up the finger-print of Cordyceps sinensis polypide, for the Cordyceps sinensis true and false, differentiates objective, accurate, easy, analytical approach is fast provided.
Accompanying drawing explanation
The standard spectrum of Fig. 1 Cordyceps sinensis polypide sample.
Fig. 2 Cordyceps gunnii (Berk.) Berk polypide infrared spectrum.
Embodiment
The Infrared spectroscopy authentication method of Cordyceps sinensis polypide provided by the invention, comprises the following steps:
A, Cordyceps sinensis polypide standard spectrum draw: choose 15 parts of Yushu district, Qinghai states |, the Cordyceps sinensis polypide sample in 15 parts of Forage Land In Guoluo Prefectures, 15 parts of Hai Nanzhou, 15 parts of Tibet, 15 parts of Sichuan and 8 parts of Gansu, the uniform sample of each provenance selection, adopt pellet technique to carry out transmission test, the averaged spectrum of all samples is Cordyceps sinensis polypide standard spectrum;
The mid-infrared spectral data processing of the present invention and Spectrum (v6) and AssureID (v4) software of analyzing use U.S. PerkinElmer company.The sample infrared spectrum initially recording is transmitance figure, comprises the spectrum baseline wander that scattering causes, the spectral intensity difference error factors that amount of samples causes, and need to be eliminated by spectrogram pre-service measures such as baseline correction, normalization.Therefore, use successively the ordinate of Spectrum software to change (transmitance is converted to absorbance), automatic baseline correction, normalization (in full spectral range, high absorbance is 1, and minimum absorbance is 0) function is processed original transmitance spectrogram.The spectrum computing function of use Spectrum software obtains the averaged spectrum of 3～5 replication spectrum of each sample, as the spectrum of this sample.According to the infrared spectrum of all samples, can calculate its averaged spectrum, as the standard spectrum of Cordyceps sinensis (polypide).
The collection of b, the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum and the common characteristic peak of Cordyceps sinensis polypide infrared spectrum.
Use Spectrum software to read each sample diffuse reflectance infrared spectroscopy peak position, peak intensity threshold value is the strength difference minimum value of the adjacent peak valley of 0.01A(and both sides), use maximum value process (Interpolated Peak) is determined in peak position.Use the characteristic peak positions of Excel or other software analysis all samples.
The diffuse reflectance infrared spectroscopy peak comparison of c, tested Cordyceps sinensis polypide sample and the comparison of infrared spectrum similarity: in the infrared spectrum of sample, comprise the indispensable characteristic peak of whole Cordyceps sinensis polypide infrared spectrums and the more than 60% of the common characteristic peak of Cordyceps sinensis polypide infrared spectrum, and the level of significance between sample spectrum and Cordyceps sinensis polypide standard spectrum is that the related coefficient lower threshold of 0.01 o'clock is 0.95, and this sample is genuine piece Cordyceps sinensis polypide.
Wherein, in the step a of the Infrared spectroscopy authentication method of above-mentioned Cordyceps sinensis polypide, described Cordyceps sinensis polypide sample is by the natural drying rear pulverizing of the polypide part of Cordyceps sinensis, gets fine powder after 200 mesh sieves for examination of infrared spectrum.
Wherein, in the step a of said method, the standard Cordyceps sinensis polypide sample in a plurality of different places of production is pressed into respectively thin slice and carries out transmission when test, carries out under the same conditions compressing tablet test, as spectral background with the pure potassium bromide that does not add any sample.
Wherein, in the step a of said method, the transmitance maximal value of every sample infrared spectrum and minimum value differ and should be not less than 60%.
Wherein, in the step a of said method, Infrared spectrum scanning scope is 4000～400cm
-1, spectral resolution is 4cm
-1, every spectrum cumulative scan 16 times or 32 times, deducts in real time carbon dioxide and water vapor and disturbs.
Wherein, in the step b of said method, the indispensable characteristic peak of described Cordyceps sinensis polypide infrared spectrum refers to the infrared spectrum absorpting peak position of comparison all samples, the characteristic peak that the absorption peak all occurring in all samples must be had as Cordyceps sinensis polypide infrared spectrum.
Further, in the step b of said method, the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum has 14.
Wherein, in the step b of said method, the described common characteristic peak of Cordyceps sinensis polypide infrared spectrum refers in the infrared spectrum absorpting peak of all samples, except indispensable characteristic peak, probability of occurrence is not less than to 90% absorption peak, as the common characteristic peak of Cordyceps sinensis polypide infrared spectrum.
Further, in the step b of said method, the common characteristic peak of Cordyceps sinensis polypide infrared spectrum has 4.
Wherein, in the step c of said method, described related coefficient is calculated by following formula:
In formula 1, R
ijrepresent the related coefficient of infrared spectrum i and infrared spectrum j, x
ikand x
jkrepresentative sample i and sample j are at the absorbance at wave number k place respectively.
In step b, the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum is in Table 3.
The indispensable characteristic peak of table 3 Cordyceps sinensis polypide infrared spectrum
In step b, the common characteristic peak of Cordyceps sinensis polypide infrared spectrum is in Table 4.
The common characteristic peak of table 4 Cordyceps sinensis polypide infrared spectrum
The present invention uses AssureID software to calculate 1800～800cm
-1in scope, the related coefficient of each sample infrared spectrum and standard spectrum, adds up the distributed area of the related coefficient of each sample infrared spectrum and standard spectrum, determines that level of significance is the related coefficient lower threshold of 0.01 o'clock.
Characteristic peak is differentiated the position of only having considered common characteristic peak, does not relate to intensity and the shape information at common characteristic peak, does not also relate to the information of other absorption peaks outside the common characteristic peak that the frequency of occurrences is higher, and needs characteristic peak number relatively more.Therefore, except above-mentioned according to common characteristic peak, differentiate, also need to differentiate according to infrared spectrum similarity, namely with correlation coefficient process, the global similarity degree between sample spectra and standard spectrum is carried out to single quantification comparison.
In the present invention, standard spectrum used is the averaging spectrum of all samples spectrum, because position and the intensity change of some absorption peaks in different samples are larger, when carrying out the discriminating of infrared spectrum similarity, has only chosen 1800～800cm
-1spectrum in scope, this district inclusion the topmost characteristic peak of sample, and spectrum change is subject to ectocine less.Related coefficient between sample spectra and Cordyceps sinensis polypide standard spectrum is carried out to statistical study, determine that level of significance is that the related coefficient lower threshold of 0.01 o'clock is 0.95.That is to say, 99% in the situation that, if testing sample is genuine piece Cordyceps sinensis polypide, the related coefficient of its infrared spectrum and standard spectrum is not less than 0.95.
Embodiment 1 Cordyceps sinensis polypide standard spectrum drafting, the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum and the collection of the common characteristic peak of Cordyceps sinensis polypide infrared spectrum and determining of related coefficient lower threshold
1) sample source and processing
Choose 15 parts of Yushu district, Qinghai states |, the Cordyceps sinensis polypide sample in 15 parts of Forage Land In Guoluo Prefectures, 15 parts of Hai Nanzhou, 15 parts of Tibet, 15 parts of Sichuan and 8 parts of Gansu, the uniform sample of each provenance selection.
Sample preparation is with reference to Pharmacopoeia of People's Republic of China relevant regulations, by the natural drying rear pulverizing of sample polypide part, gets fine powder after 200 mesh sieves for examination of infrared spectrum.
2) infrared spectrum instrument
In the present invention, the Spectrum One Fourier transformation infrared spectrometer of U.S. PerkinElmer company is all used in all samples test.
Sample test parameter: spectral scan scope 4000～400cm
-1, spectral resolution 4cm
-1, every spectrum cumulative scan 16 times or 32 times, deducts in real time carbon dioxide and water vapor and disturbs.
3) examination of infrared spectrum method
Sample test adopts potassium bromide (KBr) pressed disc method.Get 1～2 milligram of sample powder and 100 milligrams of spectroscopic pure potassium bromide broken grains ground and mixed in agate mortar is even, with sheeter, mixed-powder is pressed into thin rounded flakes, put into sample holder of Infrared spectrometer and carry out transmission test.With the pure potassium bromide that does not add any sample, carry out under the same conditions compressing tablet test, as spectral background.
The transmitance maximal value of every sample infrared spectrum and minimum value differ and should be not less than 60%.
Each sample replication 3～5 times, gets its averaged spectrum as the spectrum of this sample.
4) ir data is processed
This is studied mid-infrared spectral data processing and analyzes Spectrum (v6) and AssureID (v4) software that uses U.S. PerkinElmer company.
The sample infrared spectrum initially recording is transmitance figure, comprises the spectrum baseline wander that scattering causes, the spectral intensity difference error factors that amount of samples causes, and need to be eliminated by spectrogram pre-service measures such as baseline correction, normalization.Therefore, use successively the ordinate of Spectrum software to change (transmitance is converted to absorbance), automatic baseline correction, normalization (in full spectral range, high absorbance is 1, and minimum absorbance is 0) function is processed original transmitance spectrogram.
5) standard spectrum is drawn
The spectrum computing function of use Spectrum software obtains the averaged spectrum of 3～5 replication spectrum of each sample, as the spectrum of this sample.According to the infrared spectrum of all 30 samples, can calculate its averaged spectrum, as the standard spectrum of Cordyceps sinensis polypide.
6) collection of the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum and the common characteristic peak of Cordyceps sinensis polypide infrared spectrum
Use Spectrum software to read each sample diffuse reflectance infrared spectroscopy peak position in embodiment 1, peak intensity threshold value is 0.01 absorbance (with the strength difference minimum value of the adjacent peak valley in both sides), and use maximum value process (Interpolated Peak) is determined in peak position.Use the characteristic peak positions of Excel or all 30 samples of other software analysis.
Relatively the diffuse reflectance infrared spectroscopy peak position of all samples, finds that there is 14 absorption peaks and all occurs in all samples, can be used as the indispensable characteristic peak of genuine piece Cordyceps sinensis polypide infrared spectrum, as shown in table 1.
In the diffuse reflectance infrared spectroscopy peak of all samples, have 4 absorption peak probabilities of occurrence to be not less than in 27～29 sample infrared spectrums in 90%(30 sample and occur), can be used as the common characteristic peak of genuine piece Cordyceps sinensis polypide infrared spectrum, as shown in table 2.
7) related coefficient lower threshold determines
Use AssureID software to calculate 1800～800cm
-1the related coefficient of each sample infrared spectrum and standard spectrum in scope, formula is as follows:
In formula, R
ijrepresent the related coefficient of infrared spectrum i and infrared spectrum j, x
ikand x
jkdistinguish representative sample i and sample j at the absorbance at wave number k place,
Add up the distributed area of the related coefficient of each sample infrared spectrum and averaged spectrum, determine that level of significance is the related coefficient lower threshold of 0.01 o'clock.
Related coefficient between sample spectra and Cordyceps sinensis polypide standard spectrum is carried out to statistical study, determine that level of significance is that the related coefficient lower threshold of 0.01 o'clock is 0.95.That is to say, 99% in the situation that, if testing sample is genuine piece Cordyceps sinensis polypide, the related coefficient of its infrared spectrum and standard spectrum is not less than 0.95.
The discriminating of embodiment 2 Cordyceps gunnii (Berk.) Berk polypides and Cordyceps sinensis polypide
1) sample source and processing
Commercially available Cordyceps gunnii (Berk.) Berk, numbering is respectively G1, G2, G3, G4.
Sample preparation is with reference to Pharmacopoeia of People's Republic of China relevant regulations, by the natural drying rear pulverizing of sample polypide part, gets fine powder after 200 mesh sieves for examination of infrared spectrum.
2) infrared spectrum instrument
The Spectrum One Fourier transformation infrared spectrometer of U.S. PerkinElmer company is all used in all samples test.
Sample test parameter: spectral scan scope 4000～400cm
-1, spectral resolution 4cm
-1, every spectrum cumulative scan 16 times, deducts in real time carbon dioxide and water vapor and disturbs.
3) examination of infrared spectrum method
Sample test adopts potassium bromide (KBr) pressed disc method.Get 1～2 milligram of sample powder and 100 milligrams of spectroscopic pure potassium bromide broken grains ground and mixed in agate mortar is even, with sheeter, mixed-powder is pressed into thin rounded flakes, put into sample holder of Infrared spectrometer and carry out transmission test.With the pure potassium bromide that does not add any sample, carry out under the same conditions compressing tablet test, as spectral background.
The transmitance maximal value of every sample infrared spectrum and minimum value differ and should be not less than 60%.
Each sample replication 3～5 times, gets its averaged spectrum as the spectrum of this sample.
4) ir data is processed
This is studied mid-infrared spectral data processing and analyzes Spectrum (v6) software that uses U.S. PerkinElmer company.
The sample infrared spectrum initially recording is transmitance figure, comprises the spectrum baseline wander that scattering causes, the spectral intensity difference error factors that amount of samples causes, and need to be eliminated by spectrogram pre-service measures such as baseline correction, normalization.Therefore, use successively the ordinate of Spectrum software to change (transmitance is converted to absorbance), automatic baseline correction, normalization (in full spectral range, high absorbance is 1, and minimum absorbance is 0) function is processed original transmitance spectrogram.
5) Cordyceps gunnii (Berk.) Berk polypide diffuse reflectance infrared spectroscopy peak gather, with the comparison of Cordyceps sinensis polypide standard spectrum characteristic peak
Use Spectrum software to read each sample diffuse reflectance infrared spectroscopy peak position in embodiment 2, peak intensity threshold value is 0.01 absorbance (with the strength difference minimum value of the adjacent peak valley in both sides), use maximum value process (Interpolated Peak) is determined in peak position, and result is as shown in table 5.
Table 5 Cordyceps gunnii (Berk.) Berk polypide diffuse reflectance infrared spectroscopy peak
6) Cordyceps gunnii (Berk.) Berk and the comparison of Cordyceps sinensis polypide standard spectrum characteristic peak
Table 6 Cordyceps gunnii (Berk.) Berk and the peak comparison of Cordyceps sinensis polypide diffuse reflectance infrared spectroscopy
Table 6 is depicted as in Cordyceps gunnii (Berk.) Berk polypide diffuse reflectance infrared spectroscopy peak the part of conform to Cordyceps sinensis polypide standard spectrum characteristic peak (in table 1 and table 2 characteristic peak 99% fiducial interval).All there is not the indispensable characteristic peak of whole Cordyceps sinensis polypides in four Cordyceps gunnii (Berk.) Berk polypides, illustrates that the method can effectively carry out true and false discriminating.
Claims (6)
1. the Infrared spectroscopy authentication method of Cordyceps sinensis polypide, is characterized in that: the method comprises the following steps:
A, Cordyceps sinensis polypide standard spectrum are drawn: the standard Cordyceps sinensis polypide sample in a plurality of different places of production is pressed into respectively thin slice and carries out transmission test, and the averaged spectrum of all samples is Cordyceps sinensis polypide standard spectrum;
The collection of b, the indispensable characteristic peak of Cordyceps sinensis polypide infrared spectrum and the common characteristic peak of Cordyceps sinensis polypide infrared spectrum;
The diffuse reflectance infrared spectroscopy peak comparison of c, tested Cordyceps sinensis polypide sample and the comparison of infrared spectrum similarity: in the infrared spectrum of sample, comprise the common characteristic peak of Cordyceps sinensis polypide the infrared spectrum more than indispensable characteristic peak and 60% of whole Cordyceps sinensis polypide infrared spectrums, and the related coefficient between sample spectrum and Cordyceps sinensis polypide standard spectrum is that level of significance is that the related coefficient lower threshold of 0.01 o'clock is 0.95, and this sample is genuine piece Cordyceps sinensis polypide.
2. the Infrared spectroscopy authentication method of Cordyceps sinensis polypide according to claim 1, it is characterized in that: in step b, the indispensable characteristic peak of described Cordyceps sinensis polypide infrared spectrum refers to the infrared spectrum absorpting peak position of comparison all samples, the indispensable characteristic peak using the absorption peak all occurring in all samples as Cordyceps sinensis polypide infrared spectrum.
3. the Infrared spectroscopy authentication method of Cordyceps sinensis polypide according to claim 2, is characterized in that: the indispensable characteristic peak of described Cordyceps sinensis polypide infrared spectrum is as shown in table 1:
The indispensable characteristic peak of table 1 Cordyceps sinensis herb infrared spectrum
4. the Infrared spectroscopy authentication method of Cordyceps sinensis polypide according to claim 1, it is characterized in that: in step b, the described common characteristic peak of Cordyceps sinensis polypide infrared spectrum refers in the infrared spectrum absorpting peak of all samples, except indispensable characteristic peak, probability of occurrence is not less than to 90% absorption peak, as the common characteristic peak of Cordyceps sinensis polypide infrared spectrum.
5. the Infrared spectroscopy authentication method of Cordyceps sinensis polypide according to claim 4, is characterized in that: the common characteristic peak of described Cordyceps sinensis polypide infrared spectrum is as shown in table 2:
The common characteristic peak of table 2 Cordyceps sinensis herb infrared spectrum
6. the Infrared spectroscopy authentication method of Cordyceps sinensis polypide according to claim 1, is characterized in that: in step c, the computing formula of described related coefficient is:
wherein, R
ijrepresent the related coefficient of infrared spectrum i and infrared spectrum j, x
ikand x
jkrepresentative sample i and sample j are at the absorbance at wave number k place respectively.
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| CN201310690032.4A CN103760126B (en) | 2013-12-16 | 2013-12-16 | The Infrared spectroscopy authentication method of Cordyceps sinensis polypide |
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