CN103105374A - Method for quickly identifying true and false cordyceps mycelia - Google Patents
Method for quickly identifying true and false cordyceps mycelia Download PDFInfo
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- CN103105374A CN103105374A CN2011103613704A CN201110361370A CN103105374A CN 103105374 A CN103105374 A CN 103105374A CN 2011103613704 A CN2011103613704 A CN 2011103613704A CN 201110361370 A CN201110361370 A CN 201110361370A CN 103105374 A CN103105374 A CN 103105374A
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Abstract
The invention discloses a method for quickly identifying true and false cordyceps mycelia. The method comprises the following steps of: (1) tabletting and scanning a potassium bromide blank film to obtain a standard sample spectrum; (2) tabletting and scanning a sample, namely accurately weighing the cordyceps mycelium which is crushed and screened via a 80-meshe sieve and fine potassium bromide powder, grinding and uniformly mixing the two powder, uniformly laying the mixed powder in a pressure membrane, and pressurizing the mixed powder for 1 min so as to obtain a sample sheet, and carrying out Sample scanning on the pressed sample sheet into a fourier infrared spectrometer scanning chamber, thus obtaining a cordyceps mycelium sample spectrum; and (3) comparing the similarity of the cordyceps mycelium sample spectrum and a standard sample spectrum, wherein the criterion is that the similarity of the cordyceps mycelia is higher than 0.95. The method disclosed by the invention can be used for quickly identifying the true and false cordyceps mycelia on the basis of the difference of spectral absorption of the cordyceps mycelium; the detection is fast, samples are not damaged, and the use quantity of the cordyceps mycelia is little; and the samples are subjected to full wave infrared spectrum scanning, so that the information of the samples is complete.
Description
Technical field
The present invention relates to a kind of method of the quick discriminating cordyceps mycelia true and false.
Background technology
Cordyceps mycelia is to utilize bioseparation technology to extract effective strain from natural cordyceps, then inoculates, cultivates in the fluid nutrient medium of large volume, makes the resulting product of Chinese caterpillar fungus hypha raised growth (fermentation).
The cordyceps mycelia that obtains by fermentation and natural cs be through strict test, and they are on function and effect and indistinction, but production cost reduces greatly.Cordyceps mycelia is effective substitute of Cordyceps sinensis, it has the function identical with Cordyceps sinensis: enter lung, liver, kidney channel, have tonifying lung, kidney-nourishing, strong liver, beneficial gas, mend smart effect, have no side effect, can improve the immunologic function of human body, can also antifatigue, decompose the tired material in muscle, the human body endurance is increased, improve the locomitivity of body.
The detection that detects cordyceps mycelia is at present carried out assay mainly for functional component adenosine, two kinds of indexs of sweet mellow wine, but these two content's index can only illustrate the quality of cordyceps mycelia, the foundation of the true and false is judged in conduct according to this, so cordyceps mycelia can't effectively carry out true and false quality evaluation as pulverized specimen.
Infrared spectrum technology and relevant technologies can be used as the method more fast and effectively of Chinese medicine identification (not) and quality monitoring.Its fundamental basis is that molecular spectrum has fingerprint.For a mixture system, in its molecular spectrum, peak position, peak shape, peak intensity are representing the stack at the spectrum peak of contained corresponding various groups in system, the variation of compositions of mixtures will cause the variation of the whole spectrogram of molecular spectrum, therefore just consist of macroscopical fingerprint of spectrogram.Utilize this macroscopical fingerprint, can be directly or slightly make mathematics and process and to carry out evaluation (not) and the quality control of material.This is the basis of molecular spectrum macroscopic view fingerprint technique just.Obviously, this is a kind of method of not only having paid attention to the microcosmic fingerprint but also having emphasized macroscopical fingerprint.It utilizes fully the various technology of molecular spectrum and combines to achieve the goal with Chemical Measurement, computational mathematics method and computer technology.
The characteristics of molecular spectrum macroscopic view fingerprint technique are:
The state of 1 tested sample is without overcritical, gaseous state, liquid and solid-stately all can.Therefore can accomplish not lose former person's character, non-destructive for the various forms of Chinese medicine and the measurement of preparation.
2, easy, quick, sample is only made physics pulverize simple processing the such as compressing tablet, need not to do very loaded down with trivial details leaching separation process can directly test.
3, instrument is comparatively general, measures easy and simple to handlely, is easy to apply.
4, to material carry out to be that full constituent is whole measure, acquisition be the overall picture finger print information that contains the microcosmic fingerprint.
But there is no at present with the report of infrared spectrum technology to the technology of the cordyceps mycelia true and false.
Summary of the invention
A kind of method that the purpose of this invention is to provide quick discriminating cordyceps mycelia true and false.
Technical scheme of the present invention is summarized as follows:
A kind of method of the quick discriminating cordyceps mycelia true and false comprises the steps:
(1) compressing tablet of potassium bromide blank and scanning: precision takes dry potassium bromide fine powder 200mg; Be placed in agate mortar and fully be ground, in the press mold of diameter 13mm, paving cloth is even, is forced into 0.8Gpa with potassium bromide fine powder dislocation, keeps 1min, the potassium bromide blank of making, and the visual examination homogeneous transparent is without obvious particle; The potassium bromide blank that presses is put into the Fourier infrared spectrograph scanning room carry out Background scanning, spectrogram gets standard samples; (2) sample compressing tablet and scanning: precision takes cordyceps mycelia medicinal material 3mg and the accurate potassium bromide fine powder 200mg that takes drying through pulverizing 80 mesh sieves, being placed in agate mortar fully is ground, with the fine powder dislocation in the press mold of diameter 13mm, paving cloth is even, be forced into 0.8～1.0Gpa, keep 1min, make sample strip, visual examination should be even, without obvious particle; The sample strip that presses is put into the Fourier infrared spectrograph scanning room carry out Sample scanning, obtain cordyceps mycelia sample spectrogram;
(3) cordyceps mycelia sample spectrogram and standard model spectrogram are carried out similarity relatively, the cordyceps mycelia similarity reaches the criterion greater than 0.95.
The method of profit of the present invention differentiates fast that with the otherness of cordyceps mycelia in spectral absorption the cordyceps mycelia true and false is good and bad.
The advantage of the inventive method:
1, detect fast, the sample compressing tablet carries out infrared scan, carries out data analysis, and whole process only needs a few minutes;
2, sample nondestructive: sample size only needs 3 milligrams, and use amount is few;
3, information is comprehensive: sample carries out the all-wave Infrared spectrum scanning, and sample message is complete.
Description of drawings
Fig. 1 is cordyceps mycelia standard model infrared spectrum.
Fig. 2 is differentiated cordyceps mycelia sample infrared spectrum.
Fig. 3 is the structured flowchart of FTIR spectrometer.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
A kind of method of the quick discriminating cordyceps mycelia true and false is characterized in that comprising the steps:
(1) compressing tablet of potassium bromide blank and scanning: precision takes dry potassium bromide fine powder 200mg; Be placed in agate mortar and fully be ground, in the press mold of diameter 13mm, paving cloth is even, is forced into 0.8Gpa with potassium bromide fine powder dislocation, keeps 1min, the potassium bromide blank of making, and the visual examination homogeneous transparent is without obvious particle; The potassium bromide blank that presses is put into the Fourier infrared spectrograph scanning room carry out Background scanning, the spectrogram that gets standard samples (Fig. 1);
(2) sample compressing tablet and scanning: precision takes cordyceps mycelia medicinal material 3mg and the accurate potassium bromide fine powder 200mg that takes drying through pulverizing 80 mesh sieves, being placed in agate mortar fully is ground, with the fine powder dislocation in the press mold of diameter 13mm, paving cloth is even, be forced into 1.0Gpa, keep 1min, make sample strip, visual examination should be even, without obvious particle; The sample strip that presses is put into the Fourier infrared spectrograph scanning room carry out Sample scanning, obtain cordyceps mycelia sample spectrogram; (Fig. 2);
(3) cordyceps mycelia sample spectrogram and standard model spectrogram are carried out similarity relatively, the cordyceps mycelia similarity reaches the criterion greater than 0.95.
Embodiment 2
A kind of method of the quick discriminating cordyceps mycelia true and false is characterized in that comprising the steps:
(1) compressing tablet of potassium bromide blank and scanning: precision takes dry potassium bromide fine powder 200mg; Be placed in agate mortar and fully be ground, in the press mold of diameter 13mm, paving cloth is even, is forced into 0.8Gpa with potassium bromide fine powder dislocation, keeps 1min, the potassium bromide blank of making, and the visual examination homogeneous transparent is without obvious particle; The potassium bromide blank that presses is put into the Fourier infrared spectrograph scanning room carry out Background scanning, spectrogram gets standard samples;
(2) sample compressing tablet and scanning: precision takes cordyceps mycelia medicinal material 3mg and the accurate potassium bromide fine powder 200mg that takes drying through pulverizing 80 mesh sieves, being placed in agate mortar fully is ground, with the fine powder dislocation in the press mold of diameter 13mm, paving cloth is even, be forced into 0.8Gpa, keep 1min, make sample strip, visual examination should be even, without obvious particle; The sample strip that presses is put into the Fourier infrared spectrograph scanning room carry out Sample scanning, obtain cordyceps mycelia sample spectrogram;
(3) cordyceps mycelia sample spectrogram and standard model spectrogram are carried out similarity relatively, the cordyceps mycelia similarity reaches the criterion greater than 0.95.
Claims (1)
1. a method of differentiating fast the cordyceps mycelia true and false, is characterized in that comprising the steps:
(1) compressing tablet of potassium bromide blank and scanning: precision takes dry potassium bromide fine powder 200mg; Be placed in agate mortar and fully be ground, in the press mold of diameter 13mm, paving cloth is even, is forced into 0.8Gpa with potassium bromide fine powder dislocation, keeps 1min, the potassium bromide blank of making, and the visual examination homogeneous transparent is without obvious particle; The potassium bromide blank that presses is put into the Fourier infrared spectrograph scanning room carry out Background scanning, spectrogram gets standard samples;
(2) sample compressing tablet and scanning: precision takes cordyceps mycelia medicinal material 3mg and the accurate potassium bromide fine powder 200mg that takes drying through pulverizing 80 mesh sieves, being placed in agate mortar fully is ground, with the fine powder dislocation in the press mold of diameter 13mm, paving cloth is even, be forced into 0.8～1.0Gpa, keep 1min, make sample strip, visual examination should be even, without obvious particle; The sample strip that presses is put into the Fourier infrared spectrograph scanning room carry out Sample scanning, obtain cordyceps mycelia sample spectrogram;
(3) cordyceps mycelia sample spectrogram and standard model spectrogram are carried out similarity relatively, the cordyceps mycelia similarity reaches the criterion greater than 0.95.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103411915A (en) * | 2013-07-26 | 2013-11-27 | 江西济民可信金水宝制药有限公司 | Infrared spectroscopy identification method of traditional Chinese medicine preparation-Jinshuibao capsule |
| CN103674885A (en) * | 2013-12-06 | 2014-03-26 | 中国烟草总公司山东省公司 | Lossless rapid method for discriminating true and false cigarettes by applying infrared spectroscopy technology |
| CN103674887A (en) * | 2013-12-16 | 2014-03-26 | 张雪峰 | Analysis identification method of ophiocordyceps sinensis through infrared spectrum |
| CN103698296A (en) * | 2013-12-16 | 2014-04-02 | 张雪峰 | Infrared spectroscopic analysis and authentication method for cordyceps sinensis stromata |
| CN103954581A (en) * | 2014-03-31 | 2014-07-30 | 温州市工业科学研究院 | Method for discriminating truth of edible rosin |
| CN108802219A (en) * | 2018-06-04 | 2018-11-13 | 广东医科大学 | A kind of discrimination method of wild cordyceps and Fermented Cordyceps |
| CN111337448A (en) * | 2020-03-26 | 2020-06-26 | 杭州老爸标准技术集团有限公司 | Method for identifying cosmetics based on infrared spectrum technology |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103411915A (en) * | 2013-07-26 | 2013-11-27 | 江西济民可信金水宝制药有限公司 | Infrared spectroscopy identification method of traditional Chinese medicine preparation-Jinshuibao capsule |
| CN103674885A (en) * | 2013-12-06 | 2014-03-26 | 中国烟草总公司山东省公司 | Lossless rapid method for discriminating true and false cigarettes by applying infrared spectroscopy technology |
| CN103674887A (en) * | 2013-12-16 | 2014-03-26 | 张雪峰 | Analysis identification method of ophiocordyceps sinensis through infrared spectrum |
| CN103698296A (en) * | 2013-12-16 | 2014-04-02 | 张雪峰 | Infrared spectroscopic analysis and authentication method for cordyceps sinensis stromata |
| CN103674887B (en) * | 2013-12-16 | 2016-01-27 | 青海春天药用资源科技利用有限公司 | The Infrared spectroscopy authentication method of Cordyceps sinensis herb |
| CN103698296B (en) * | 2013-12-16 | 2016-06-01 | 青海春天药用资源科技利用有限公司 | The Infrared spectroscopy authentication method of Cordyceps sinensis stroma |
| CN103954581A (en) * | 2014-03-31 | 2014-07-30 | 温州市工业科学研究院 | Method for discriminating truth of edible rosin |
| CN108802219A (en) * | 2018-06-04 | 2018-11-13 | 广东医科大学 | A kind of discrimination method of wild cordyceps and Fermented Cordyceps |
| CN108802219B (en) * | 2018-06-04 | 2019-08-06 | 广东医科大学 | A kind of discrimination method of wild cordyceps and Fermented Cordyceps |
| CN111337448A (en) * | 2020-03-26 | 2020-06-26 | 杭州老爸标准技术集团有限公司 | Method for identifying cosmetics based on infrared spectrum technology |
| CN111337448B (en) * | 2020-03-26 | 2023-05-12 | 杭州老爸评测科技股份有限公司 | Method for identifying cosmetics based on infrared spectrum technology |
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