CN109932332A - A kind of method of infrared spectroscopy detection Chinese medicine - Google Patents
A kind of method of infrared spectroscopy detection Chinese medicine Download PDFInfo
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Abstract
The invention belongs to Chinese medicine detection technique fields, provide a kind of method of infrared spectroscopy detection Chinese medicine.This method is specifically the method for utilizing infrared spectroscopy to detect adulterated angelica sinensis and adulterated Radix Codonopsis medicine materical crude slice and the identification of astragalus mongolicus medicine materical crude slice.The present invention utilizes infrared spectrometer, data analysis is carried out in conjunction with infared spectrum Correlative characteristic peaks and simple algorithm, can have in Radix Angelicae Sinensis sample can specify Direct Identification when mixing lovage 5%, quantitative verification is also carried out by high performance liquid chromatography, entire discrimination process does not destroy sample, quickly identify, accurate stable, additionally it is possible to windproof detection and the identification astragalus mongolicus true and false are mixed in Radix Codonopsis medicine materical crude slice applied to identifying.
Description
Technical field
The invention belongs to Chinese medicine detection technique fields, are related to a kind of method of infrared spectroscopy detection Chinese medicine, specifically
The method for detecting adulterated angelica sinensis and adulterated Radix Codonopsis medicine materical crude slice and Radix Astragali medicine materical crude slice using infrared spectroscopy.
Background technique
For a long time, China's majority Chinese medicine Habitat producing is dispersion processing, after place of production preparatory processing is at crude drug, respectively
Regional, each enterprise secondary operation again.Lack standardization production for producing implementation condition difference and various regions, especially individually
Square sulfur fumigation remains incessant after repeated prohibition, and causes the prepared slice quality processed irregular, and market is adulterated also commonplace, affects
The performance of Clinical Action of Traditional Chinese Drugs.
Infra-red sepectrometry is also known as " ir spectrophotometry analytic approach ".It is one kind of molecular absorption spectrum.According to different material
The electromagnetic radiation of infrared light district can selectively be absorbed to carry out structural analysis；To the various compounds for absorbing infrared light
A kind of method qualitatively and quantitatively analyzed.Substance is the atomic building of the state by constantly vibrating, these atomic vibration frequencies with
The vibration frequency of infrared light is suitable.When with Infrared irradiation organic matter, vibration level transition can occur for molecule absorption infrared light,
Different chemical bonds or functional group's absorption frequency is different, and each organic molecule only absorbs and its molecular vibration, rotational frequency phase
Consistent infrared spectroscopy, obtained absorption spectrum are commonly referred to as infrared absorption spectrum, abbreviation infrared spectroscopy " IR ".To infrared
Spectrum is analyzed, and can carry out qualitative analysis to substance.The content of each substance also will reflect on infrared absorption spectrum, can
Quantitative analysis is carried out according to peak position, absorption intensity.
In recent years, the prepared slices of Chinese crude drugs are adulterated happens occasionally, such as radix saposhnikoviae, radix stellariae dichotomae are mixed in Radix Codonopsis, for another example adulterated Europe in Radix Angelicae Sinensis
Radix Angelicae Sinensis, Radix Angelicae Sinensis, lovage are all umbelliferae, and appearance is extremely similar, also had in test Radix Angelicae Pubescentis a small amount of volatile oil, Ah
Wei's sour component, therefore be more difficult to determine mixes lovage ratio inside Radix Angelicae Sinensis and reaches 40% and detects ferulaic acid content with liquid phase,
It is qualified to examine, and meets standards of pharmacopoeia.In addition, the market price through investigating adulterated lovage medicinal material in Radix Angelicae Sinensis is compared with Radix Angelicae Sinensis market
Price low 15% or so, in order to effectively prevent the adulterated situation in market, also for allowing the people to be able to eat the Chinese medicine that trusts of health
Medicine materical crude slice, we provide a kind of rapidly and efficiently, precisely identify in angelica sinensis to mix the red of radix saposhnikoviae in adulterated lovage and Radix Codonopsis
External spectrum detection method.
Traditional Chinese medicine sample contains extremely complex chemical constituent, and contained group and its quantity are different, and molecular structure is respectively not
It is identical, when infra-red radiation passes through liquid or solid traditional Chinese medicine sample, will occur the distinctive absorption of each at different wave length
The superposition of peak (band) intensity, using wavelength or wave number as abscissa, transmitance or absorbance are ordinate drawing, obtain sample
Characteristic absorption curve is infrared absorption spectrum.With the position of the macro morphology of spectrum and absorption peak, intensity and the microcosmic finger of shape
Line is used to qualitatively judge or identify sample.Certain group in sample is determined with the relative intensity of certain key bands or characteristic peak
The qualitative analysis divided is used.
Summary of the invention
The technical problem to be solved by the present invention is to be directed to prior art Shortcomings, provide a kind of using infrared spectroscopy
The method for detecting Chinese medicine especially detects adulterated angelica sinensis and adulterated Radix Codonopsis medicine materical crude slice using infrared spectroscopy and Radix Astragali is drunk
Piece identification.
In order to achieve the above object, the present invention uses following technical scheme.
A kind of method of infrared spectroscopy detection Chinese medicine, comprising the following steps:
(1) medicine materical crude slice that the certified products of different sources of the same race circulates without sulphur Chinese medicine processing medicine materical crude slice, in the market is acquired as sample；
(2) materials crushing, cross powder that 200 meshes obtain as sample to be tested, take sample to be tested and spectroscopic pure grade KBr
Broken grain is placed in agate mortar, levigate, and is uniformly mixed it, and the powder of finely ground mixing is poured into 13mm compression mold, is made
Its paving cloth is uniform, is forced into 0.8~1GPa, keeps 1min, be pressed into thin rounded flakes using tablet press machine, be then placed in infrared
Optical spectrometer sample stand is tested, before scanning sample to be tested, first with pure KBr by preceding method suppress flakiness, according to
The identical parameter setting mode of scanning sample to be tested is tested, and spectral background is obtained, and next scans the spectrum of sample to be tested
When, instrument can carry out background deduction automatically, obtain the real spectrum of sample to be tested；
(3) the certified products in sample to be tested is pressed into the place of production without sulphur Chinese medicine processing medicine materical crude slice, using baseline correction-reconnaissance normalization method
It carries out the infrared Correlative characteristic peaks of Radix Angelicae Sinensis and is made with respect to peak height line chart and Correlative characteristic peaks with respect to peak height histogram, as control
Figure, the ownership place of the Chinese medicine of the same race to circulate in the market is determined with this；
(4), according to infrared spectroscopy map, certified products is directly compared on certain wave band without sulphur Chinese medicine processing medicine materical crude slice, in the market
The Chinese medicine infrared spectroscopy map of the same race of circulation, intuition determine whether mix puppet in the tcm and herbal slice of the same race to circulate in the market；
(5) Compare is carried using infrared spectroscopy and compare analysis software, added with certified products without sulphur Chinese medicine on certain wave band
Work medicine materical crude slice control medicinal material, which does to mix, compares analysis than carrying out Compare, then by comparing the Chinese medicine of the same race for determining and circulating in the market
Pseudo- ratio is mixed in medicine materical crude slice.
Further, identify the method that lovage is mixed in angelica sinensis using the infrared spectrum detection method, including following
Step:
(1) angelica sinensis and lovage work that the certified products Radix Angelicae Sinensis of different sources circulates without sulphur processing medicine materical crude slice, in the market are acquired
For sample；
(2) materials crushing, cross powder that 200 meshes obtain as sample to be tested, take sample to be tested and spectroscopic pure grade KBr
Broken grain is placed in agate mortar, levigate, and is uniformly mixed it, and the powder of finely ground mixing is poured into 13mm compression mold, is made
Its paving cloth is uniform, is forced into 0.8~1GPa, keeps 1min, be pressed into thin rounded flakes using tablet press machine, be then placed in infrared
Optical spectrometer sample stand is tested, before scanning sample to be tested, first with pure KBr by preceding method suppress flakiness, according to
The identical parameter setting mode of scanning sample to be tested is tested, and spectral background is obtained, and next scans the spectrum of sample to be tested
When, instrument can carry out background deduction automatically, obtain the real spectrum of sample to be tested；
(3) the Radix Angelicae Sinensis in sample to be tested is pressed into the place of production without sulphur processing medicine materical crude slice, worked as using baseline correction-reconnaissance normalization method
Infrared Correlative characteristic peaks are returned to make with respect to peak height line chart and Correlative characteristic peaks with respect to peak height histogram, as comparative diagram, with this
Determine the ownership place of the angelica sinensis to circulate in the market；
(4) according to infrared spectroscopy map, in 1700-1580cm-1Wave band, directly comparison Radix Angelicae Sinensis process medicine materical crude slice, market without sulphur
The angelica sinensis infrared spectroscopy map of upper circulation, intuition determine whether mix lovage in the angelica sinensis to circulate in the market；
(5) Compare is carried using infrared spectroscopy and compare analysis software, in 1700-1580cm-1Wave band is added with Radix Angelicae Sinensis without sulphur
Work medicine materical crude slice control medicinal material, which does to mix, compares analysis than carrying out Compare, then by comparing in the angelica sinensis for determining and circulating in the market
Whether mix lovage and mixes ratio.
The infrared spectrometer is Fourier Transform Infrared Spectrometer, parameter are as follows: instrumental resolution is better than 4cm-1, base
Line noise 6000:1~2000:1, wave-number range 4000-400cm-1, baseline tilt 0.1%T~0.5%T, transmitance accuracy
0.1%T, transmitance repeatability 0.1%T~0.5%T, absorbance repeatability < 0.005.
Preferably, when infrared spectrometer scanning sample to be tested, number is 16~32 times when scanning, sample to be tested
Replication 3 times, related coefficient 0.98.
Preferably, the mass percent of the sample to be tested and spectroscopic pure grade KBr dosage is 1~2%:1, make gained
Transmitance >=60% of most strong absworption peak in infrared spectrum.
Preferably, the infrared spectroscopy testing result of adulterated lovage is carried out using high performance liquid chromatography in angelica sinensis
Quantitative verification is first measured the ferulic acid of sample using high performance liquid chromatograph, then surveys to the volatile oil of sample
Fixed, before measurement, Radix Angelicae Sinensis Authentic plant drugs mix lovage in the following proportions: No. 1-Radix Angelicae Sinensis processes medicine materical crude slice without sulphur, and No. 2-contain 5% Europe
The Radix Angelicae Sinensis of Radix Angelicae Sinensis processes medicine materical crude slice without sulphur, and No. 3-Radix Angelicae Sinensis containing 10% lovage processes medicine materical crude slice without sulphur, No. 4-containing 20% lovage
Radix Angelicae Sinensis without sulphur process medicine materical crude slice, No. 5-containing 30% lovage Radix Angelicae Sinensis without sulphur process medicine materical crude slice, No. 6-containing 40% lovage Radix Angelicae Sinensis without
Sulphur processes medicine materical crude slice, and No. 7-Radix Angelicae Sinensis containing 50% lovage processes medicine materical crude slice without sulphur, and No. 8-Radix Angelicae Sinensis containing 60% lovage is processed without sulphur
Medicine materical crude slice, No. 9-Radix Angelicae Sinensis containing 70% lovage process medicine materical crude slice without sulphur, and No. 10-Radix Angelicae Sinensis containing 100%% lovage is processed without sulphur to be drunk
Piece makes standard curve according to result, and after measurement, determination is compared with standard curve in the measured value of sample to be tested.
It is a further object of the present invention to provide the infrared spectrum detection methods to mix pseudo- radix saposhnikoviae in identifying Radix Codonopsis medicine materical crude slice
Using.
The present invention also provides the infrared spectrum detection methods to identify the application in astragalus mongolicus medicine materical crude slice.
Advantageous effects of the invention: the present invention utilizes infrared spectrometer, in conjunction with infared spectrum Correlative characteristic peaks and letter
Single algorithm carries out data analysis, and can have in Radix Angelicae Sinensis sample can specify Direct Identification when mixing lovage 5%, also pass through height
Effect liquid phase chromatogram carries out quantitative verification, and entire discrimination process does not destroy wild ginseng sample, quickly identifies, accurate stable, the infrared light
Spectrum detection method can also be applied to identify the detection for mixing radix saposhnikoviae in Radix Codonopsis medicine materical crude slice and the identification astragalus mongolicus true and false.
Detailed description of the invention
Fig. 1 is the infrared Correlative characteristic peaks of Minxian County Radix Angelicae Sinensis and Qinghai Radix Angelicae Sinensis, Yunnan Radix Angelicae Sinensis with respect to peak height line chart.
Fig. 2 is the infrared Correlative characteristic peaks of Minxian County Radix Angelicae Sinensis and Qinghai Radix Angelicae Sinensis, Yunnan Radix Angelicae Sinensis with respect to peak height histogram.
Fig. 3 is Radix Angelicae Sinensis standard medicinal material (a), Radix Angelicae Sinensis (b) and the infrared spectrogram for mixing lovage (c).
Fig. 4 is that Radix Angelicae Sinensis-mixes lovage infrared spectroscopy reconnaissance normalization figure (4000-400cm-1Wave band).
Fig. 5 is that Radix Angelicae Sinensis-mixes its 1:1 aggregate sample infrared spectroscopy normalization figure of lovage (1800-800cm-1Wave band).
Fig. 6 is that Radix Angelicae Sinensis-mixes lovage and its 1:1,3:1,5:1,10:1 and 20:1 aggregate sample infrared spectroscopy normalization figure
(1700-1580cm-1Wave band).
Fig. 7 is the infrared spectrogram of Radix Codonopsis (a) and windproof (b).
Fig. 8 is infrared spectrogram of the astragalus mongolicus without sulphur processing medicine materical crude slice (reference substance).
Specific embodiment
Technical solution of the present invention is clearly and completely described below with reference to embodiment, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.
The infrared spectrum detection method of adulterated lovage in embodiment angelica sinensis
1, instrument and environmental condition
Fourier Transform Infrared Spectrometer: instrumental resolution is better than 4cm-1, baseline noise 6000:1~2000:1, wave number
Range 4000-400cm-1, baseline tilt 0.1%T~0.5%T, transmitance accuracy 0.1%T, transmitance repeatability 0.1%T
~0.5%T, absorbance repeatability < 0.005.
Room temperature should control within the scope of 20~25 DEG C, humidity < 60%.
2, sample and reagent
The angelica sinensis and lovage that the certified products of Radix Angelicae Sinensis circulates without sulphur processing medicine materical crude slice, in the market.Radix Angelicae Sinensis sample is acquired from various regions
390 kinds of product, wherein 100 kinds of Minxian County Radix Angelicae Sinensis, 40 kinds of Yunnan Radix Angelicae Sinensis, 40 kinds of Qinghai Radix Angelicae Sinensis.
Excellent pure grade or spectroscopic pure grade potassium bromide (KBr).
Reference substance, control medicinal material and standard items (internal standard substance including chromatographic grade) are referred to by drug regulatory department
The fixed system of unit is standby, demarcates and supplies.
3, sample preparation
(1) Radix Angelicae Sinensis processes medicine materical crude slice control sample without sulphur
It is sampled according to Chinese Pharmacopoeia sampling method, the powder for smashing it through (74 μm) of 200 mesh sieves directly uses KBr pressed disc method
Measurement.
(2) angelica sinensis and lovage sample to circulate in the market
It is sampled according to Chinese Pharmacopoeia sampling method, the powder for smashing it through (74 μm) of 200 mesh sieves directly uses KBr pressed disc method
Measurement.
(3) KBr pressed disc method
The KBr broken grain of the sample to be tested and 150mg that take 3mg is placed in agate mortar, levigate, and is uniformly mixed it.It will
The powder of finely ground mixing is poured into 13mm compression mold, is kept its paving cloth uniform, is forced into 0.8~1 GPa, keeps 1min, use
Tablet press machine is pressed into thin rounded flakes, is then placed in infrared spectrograph sample bracket and is tested.Before scanning sample to be tested,
Flakiness first is suppressed by preceding method with pure KBr, is tested according to parameter setting mode identical with scanning sample to be tested,
Obtain spectral background.Next when the spectrum of scanning sample to be tested, instrument can carry out background deduction automatically, obtain sample to be tested
Real spectrum.
4, determination condition
Scanning times: 16~32 times, number can suitably be increased when noise is big.
Ratio of the sample to KBr: sample accounts for 1~2% (mass percent), with most strong absworption peak in gained infrared spectrum
Transmitance reach (Y baseline-Y highest peak) >=60% and be advisable.
3 numbers of Radix Angelicae Sinensis sample replication, related coefficient are advisable 0.98.
5, the qualitative analysis of infrared spectroscopy
Such as Fig. 1 and 2, using " baseline correction-reconnaissance normalization method (1068cm-1) ", to Minxian County Radix Angelicae Sinensis, Yunnan Radix Angelicae Sinensis, Qinghai
Radix Angelicae Sinensis carries out the infrared Correlative characteristic peaks of Radix Angelicae Sinensis and makes with respect to peak height line chart and Correlative characteristic peaks with respect to peak height histogram, as
Comparative diagram determines the ownership place of the angelica sinensis to circulate in the market with this.
In conjunction with table 1, studied by the infrared Correlative characteristic peaks of Radix Angelicae Sinensis produced to Minxian County and Qinghai, Yunnan, as a result, it has been found that, it removes
Organosilane ester ingredient Min returns the Radix Angelicae Sinensis compared with Qinghai, Yunnan production slightly lower outer, Habitat producing influence, organic acid, acid esters correlation ratio,
Small molecule sucrose, macromolecular carbohydrate, acid sugar correlation ratio Min return the Radix Angelicae Sinensis for being higher than Qinghai, Yunnan production.
Table 1
6, Radix Angelicae Sinensis medicinal material and lovage are mixed than identifying
(1) it is intuitively identified according to TuPu method peak variation
Such as Fig. 3, determine from infrared spectroscopy map intuition, especially 1700-1580cm-1Wave band difference characteristic is prominent, Radix Angelicae Sinensis with
Mixing lovage has apparent feature difference.
Precision, which weighs, Radix Angelicae Sinensis sample powder and mixes lovage powder, prepare respectively Radix Angelicae Sinensis-lovage ratio be 1:1,3:1,
The aggregate sample of 5:1,10:1 and 20:1, KBr tabletting sample preparation obtain infrared spectrogram, and Radix Angelicae Sinensis mixes lovage and its 1:1 aggregate sample
Infrared signature difference is obvious, especially mixes lovage 1627cm-1Characteristic peak is prominent.With 1627cm-1Characteristic peak is reference, Radix Angelicae Sinensis,
Mix lovage and its 1:1,3:1,5:1,10:1,20:1 aggregate sample infrared spectrogram (1700-1580cm-1Wave band) at normalization
Reason, in rule performance, such as Fig. 4,5 and 6, and the 1627cm of 20:1 aggregate sample infrared spectrogram-1Characteristic peak also obviously protrudes, i.e.,
Direct Identification can be specified and judge whether to mix puppet by having in Radix Angelicae Sinensis sample when mixing lovage 5%.
(2) Comparison Method with Correlation Coefficient
Compare is carried using infrared spectroscopy and compares analysis software, is compared according to related coefficient mathematically two infrared
A series of similarity degree between spectrum (or between a spectrum and spectrum).
Select characteristic wave bands (1700-1600cm-1Wave band is done with Radix Angelicae Sinensis control medicinal material and is mixed than when progress Compare comparison
Analysis, as a result Radix Angelicae Sinensis, mix lovage and its 1:1,3:1,5:1,10:1 and 20:1 aggregate sample infrared spectroscopy related coefficient ratio have it is bright
Significant difference is different, such as table 2.
2 1700-1600cm of table-1Wave band Radix Angelicae Sinensis mixes lovage and its different proportion mixing sample Compare comparison correlation
Coefficient (does reference with Radix Angelicae Sinensis control medicinal material)
Above-mentioned Radix Angelicae Sinensis control medicinal material, that is, Radix Angelicae Sinensis certified products processes medicine materical crude slice without sulphur.
The infrared spectrum detection method of pseudo- radix saposhnikoviae is mixed in 2 Radix Codonopsis medicine materical crude slice of embodiment
1, instrument and environmental condition
With embodiment 1.
2, sample and reagent
There are west of Gansu Province informal voucher party, western party, Inner Mongol party, river party, the other the same as in Example 1 by the Radix Codonopsis that the place of production acquires.
3, sample preparation
The method of sample is prepared with embodiment 1.
4, determination condition
Scanning times: 16~32 times, number can suitably be increased when noise is big.
Ratio of the sample to KBr: sample accounts for 1~2% (mass percent), with most strong absworption peak in gained infrared spectrum
Transmitance reach (Y baseline-Y highest peak) >=60% and be advisable.
3 numbers of Radix Codonopsis sample replication, related coefficient are advisable 0.98.
5, codonopsis pilosula and the intuitive identification of pseudo- radix saposhnikoviae is mixed
Such as Fig. 7, the main absorption peak that can be seen that Radix Codonopsis (Fig. 7-a) from the infrared spectrogram of Radix Codonopsis and radix saposhnikoviae has:
3390±2cm-1、2934±2cm-1、1735±2cm-1、1638±2cm-1、1418±2cm-1、 1255±2cm-1、1059±
2cm-1、868±2cm-1、818±2cm-1、779±2cm-1With 621 ± 2cm-1；The main absorption peak of windproof (Fig. 7-b) has:
3389±2cm-1、2926±2cm-1、2855±2cm-1、 1745±2cm-1、1658±2cm-1、1416±2cm-1、1240±
2cm-1、1104±2cm-1、1052 ±2cm-1、989±2cm-1、920±2cm-1、867±2cm-1、681±2cm-1With 551 ±
2cm-1.As it can be seen that the infrared spectrogram of Radix Codonopsis and radix saposhnikoviae has apparent feature difference, can sentence from infrared spectroscopy map intuition
It is fixed.
The infrared spectrum detection method of 3 astragalus mongolicus medicine materical crude slice of embodiment
1, instrument and environmental condition
With embodiment 1.
2, sample and reagent
In the Radix Astragali of acquisition, astragalus mongolicus is reference substance without sulphur processing medicine materical crude slice, and the astragalus mongolicus to circulate in the market is test
Product.
3, sample preparation
The method of sample is prepared with embodiment 1.
4, determination condition
Scanning times: 16~32 times, number can suitably be increased when noise is big.
Ratio of the sample to KBr: sample accounts for 1~2% (mass percent), with most strong absworption peak in gained infrared spectrum
Transmitance reach (Y baseline-Y highest peak) >=60% and be advisable.
3 numbers of Radix Codonopsis sample replication, related coefficient are advisable 0.98.
5, the intuitive Qualitative Identification of astragalus mongolicus
It is the infrared spectrogram that astragalus mongolicus processes medicine materical crude slice (reference substance) without sulphur such as Fig. 8, main absorption peak has: 3378
±2cm-1、2933±2cm-1、1736±2cm-1、1643±2cm-1、1515±2cm-1、 1428±2cm-1、1376±2cm-1、
1245±2cm-1、1153±2cm-1、1077±2cm-1With 1027 ± 2cm-1.The astragalus mongolicus to be circulated in the market by detection again
Infrared spectrogram can discern the false from the genuine by comparison from infrared spectroscopy map.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of method of infrared spectroscopy detection Chinese medicine, which comprises the following steps:
(1) medicine materical crude slice that the certified products of different sources of the same race circulates without sulphur Chinese medicine processing medicine materical crude slice, in the market is acquired as sample；
(2) materials crushing, cross powder that 200 meshes obtain as sample to be tested, take sample to be tested and spectroscopic pure grade KBr broken grain
It is placed in agate mortar, it is levigate, and it is uniformly mixed it, the powder of finely ground mixing is poured into 13mm compression mold, its paving is made
Cloth is uniform, is forced into 0.8~1GPa, keeps 1min, is pressed into thin rounded flakes using tablet press machine, is then placed in infrared spectrometer
Sample holder is tested, before scanning sample to be tested, first with pure KBr by preceding method suppress flakiness, according to scanning to
The identical parameter setting mode of sample is tested, and spectral background is obtained, when next scanning the spectrum of sample to be tested, instrument
Background deduction can be carried out automatically, obtain the real spectrum of sample to be tested；
(3) the certified products in sample to be tested is pressed into the place of production without sulphur Chinese medicine processing medicine materical crude slice, carried out using baseline correction-reconnaissance normalization method
The infrared Correlative characteristic peaks of Radix Angelicae Sinensis make with respect to peak height line chart and Correlative characteristic peaks with respect to peak height histogram, as comparative diagram, with
The ownership place for the Chinese medicine of the same race that this determination is circulated in the market；
(4), according to infrared spectroscopy map, certified products is directly compared on certain wave band and is circulated without sulphur Chinese medicine processing medicine materical crude slice, in the market
Chinese medicine infrared spectroscopy map of the same race, intuition determines whether mix puppet in the tcm and herbal slice of the same race that circulates in the market；
(5) Compare is carried using infrared spectroscopy and compare analysis software, processed and drunk without sulphur Chinese medicine with certified products on certain wave band
Piece control medicinal material, which does to mix, compares analysis than carrying out Compare, then by comparing the tcm and herbal slice of the same race for determining and circulating in the market
In mix pseudo- ratio.
2. a kind of method of adulterated lovage in infrared spectroscopy detection angelica sinensis, which comprises the following steps:
(1) the angelica sinensis and lovage that the certified products Radix Angelicae Sinensis of acquisition different sources circulates without sulphur processing medicine materical crude slice, in the market, which are used as, to be tried
Sample；
(2) materials crushing, cross powder that 200 meshes obtain as sample to be tested, take sample to be tested and spectroscopic pure grade KBr broken grain
It is placed in agate mortar, it is levigate, and it is uniformly mixed it, the powder of finely ground mixing is poured into 13mm compression mold, its paving is made
Cloth is uniform, is forced into 0.8~1GPa, keeps 1min, is pressed into thin rounded flakes using tablet press machine, is then placed in infrared spectrometer
Sample holder is tested, before scanning sample to be tested, first with pure KBr by preceding method suppress flakiness, according to scanning to
The identical parameter setting mode of sample is tested, and spectral background is obtained, when next scanning the spectrum of sample to be tested, instrument
Background deduction can be carried out automatically, obtain the real spectrum of sample to be tested；
(3) the Radix Angelicae Sinensis in sample to be tested is pressed into the place of production without sulphur processing medicine materical crude slice, it is red to carry out Radix Angelicae Sinensis using baseline correction-reconnaissance normalization method
Outer Correlative characteristic peaks make with respect to peak height line chart and Correlative characteristic peaks with respect to peak height histogram, as comparative diagram, are determined with this
The ownership place of the angelica sinensis to circulate in the market；
(4) according to infrared spectroscopy map, in 1700-1580cm-1Wave band directly compares Radix Angelicae Sinensis and circulates without sulphur processing medicine materical crude slice, in the market
Angelica sinensis infrared spectroscopy map, intuition determines whether mix lovage in the angelica sinensis that circulates in the market；
(5) Compare is carried using infrared spectroscopy and compare analysis software, in 1700-1580cm-1Wave band, which is processed with Radix Angelicae Sinensis without sulphur, to be drunk
Piece control medicinal material, which does to mix, compares analysis than carrying out Compare, then is determined in the angelica sinensis to circulate in the market by comparison and mix Europe
Radix Angelicae Sinensis mixes ratio.
3. the method that infrared spectroscopy detects adulterated lovage in angelica sinensis according to claim 2, it is characterised in that: described
Infrared spectrometer is Fourier Transform Infrared Spectrometer, parameter are as follows: instrumental resolution is better than 4cm-1, baseline noise 6000:1~
2000:1, wave-number range 4000-400cm-1, baseline tilt 0.1%T~0.5%T, transmitance accuracy 0.1%T, transmitance weight
Renaturation 0.1%T~0.5%T, absorbance repeatability < 0.005；When infrared spectrometer scanning sample to be tested, number when scanning
It is 16~32 times, sample to be tested replication 3 times, related coefficient 0.98.
4. the method that infrared spectroscopy detects adulterated lovage in angelica sinensis according to claim 2, it is characterised in that: described
The mass percent of sample to be tested and spectroscopic pure grade KBr dosage is 1~2%:1, makes most strong absworption peak in gained infrared spectrum
Transmitance >=60%.
5. the method that infrared spectroscopy detects adulterated lovage in angelica sinensis according to claim 2, it is characterised in that: Radix Angelicae Sinensis
The infrared spectroscopy testing result of adulterated lovage carries out quantitative verification using high performance liquid chromatography in medicine materical crude slice, first uses efficient liquid phase
Chromatograph is measured the ferulic acid of sample, is then measured to the volatile oil of sample, before measurement, Radix Angelicae Sinensis Authentic plant drugs
Mix lovage in the following proportions: No. 1-Radix Angelicae Sinensis processes medicine materical crude slice without sulphur, and No. 2-Radix Angelicae Sinensis containing 5% lovage processes medicine materical crude slice without sulphur, and 3
Number-containing 10% lovage Radix Angelicae Sinensis without sulphur process medicine materical crude slice, No. 4-containing 20% lovage Radix Angelicae Sinensis without sulphur process medicine materical crude slice, No. 5-contain
The Radix Angelicae Sinensis of 30% lovage without sulphur process medicine materical crude slice, No. 6-containing 40% lovage Radix Angelicae Sinensis without sulphur process medicine materical crude slice, No. 7-contain 50% Europe
The Radix Angelicae Sinensis of Radix Angelicae Sinensis processes medicine materical crude slice without sulphur, and No. 8-Radix Angelicae Sinensis containing 60% lovage processes medicine materical crude slice without sulphur, No. 9-containing 70% lovage
Radix Angelicae Sinensis processes medicine materical crude slice without sulphur, and No. 10-Radix Angelicae Sinensis containing 100%% lovage processes medicine materical crude slice without sulphur, makes standard curve according to result,
After measurement, determination is compared with standard curve in the measured value of sample to be tested.
6. mixing answering for pseudo- radix saposhnikoviae in identifying Radix Codonopsis medicine materical crude slice using the method for the detection Chinese medicine of infrared spectroscopy described in claim 1
With.
7. utilizing application of the method for the detection Chinese medicine of infrared spectroscopy described in claim 1 in identification astragalus mongolicus medicine materical crude slice.
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