CN102998279A - Method for fast identifying authenticity of Ganoderma lucidum spore powder - Google Patents

Abstract

The invention discloses a method for fast identifying authenticity of Ganoderma lucidum spore powder. The method comprises the following steps of: (1) sample processing: preparing 10g of to-be-identified Ganoderma lucidum spore crude drugs, crushing the to-be-identified Ganoderma lucidum spore crude drugs, and enabling the to-be-identified Ganoderma lucidum spore crude drugs to pass through a 80-mesh screen for standby; (2) instrument: a Fourier infrared spectrometer; (3) blank scanning; (4) standard substance scanning; (5) sample scanning; and (6) similarity comparison. The method for fast identifying the authenticity of Ganoderma lucidum spore powder has the advantages that the detection is fast, and the whole process of tabletting, infrared scanning and data analysis of the sample only needs several minutes; the sample is lossless: the sample amount is only 3mg, and the usage amount is precious little; and the information is comprehensive: the sample is subjected to full-wave infrared spectroscopy scanning, and the sample information is complete.

Description

A kind of method of the quick discriminating lucidum spore powder true and false

Technical field

The present invention relates to a kind of method of the quick discriminating lucidum spore powder true and false.

Background technology

Glossy ganoderma is the dry fructification of Polyporaceae plant glossy ganoderma Ganoderma lucidum (Leyss.Ex.Fr) Karst, is a kind of medicinal fungi.It is the treasure in the traditional Chinese medicine and pharmacy treasure-house, contains the number of chemical composition, can be used for preventing and treating the various diseases such as chronic bronchitis, neurasthenia, hyperlipemia, coronary heart disease, hepatitis, leukopenia and tumour.Therefore how scientifically to study glossy ganoderma, reasonably application and correct evaluation glossy ganoderma are the important subject of attracting attention both at home and abroad.

At present the detection of lucidum spore powder is carried out assay mainly for functional component adenosine, two kinds of indexs of sweet mellow wine, but these two content's index can only illustrate the quality of lucidum spore powder, can not be with this as the foundation of judging the true and false, so lucidum spore powder can't effectively carry out true and false quality evaluation as pulverized specimen.

Infrared spectrum technology and relevant technologies can be used as the more fast and effectively method of Chinese medicine identification (not) and quality monitoring.Its fundamental basis is that molecular spectrum has fingerprint.For a mixture system, peak position, peak shape, peak intensity are then representing the stack at the spectrum peak of contained corresponding various groups in the system in the molecular spectrum, the variation of compositions of mixtures will cause the variation of the whole spectrogram of molecular spectrum, therefore just consist of macroscopical fingerprint of spectrogram.Utilize this macroscopical fingerprint, can be directly or slightly make mathematics and process and to carry out evaluation (not) and the quality control of material.This is the basis of molecular spectrum macroscopic view fingerprint technique just.Obviously, this is a kind of method of not only having paid attention to the microcosmic fingerprint but also having emphasized macroscopical fingerprint.It utilizes fully the various technology of molecular spectrum and combines to achieve the goal with Chemical Measurement, computational mathematics method and computer technology.

The characteristics of molecular spectrum macroscopic view fingerprint technique are:

The state of 1 tested sample is without overcritical, gaseous state, liquid and solid-stately all can.Therefore can accomplish not lose former person's character, non-destructive for the various forms of Chinese medicine and the measurement of preparation.

2, easy, quick, sample is only made physics pulverize simple processing the such as compressing tablet, need not to do very loaded down with trivial details leaching separation process can directly test.

3, instrument is comparatively general, measures easy and simple to handlely, is easy to apply.

4, to material carry out to be that full constituent is whole measure, acquisition be the overall picture finger print information that contains the microcosmic fingerprint.

There is no at present with the report of infrared spectrum technology to the technology of the lucidum spore powder true and false.

Summary of the invention

The method that the purpose of this invention is to provide a kind of quick discriminating lucidum spore powder true and false.

Technical scheme of the present invention is summarized as follows:

A kind of method of the quick discriminating lucidum spore powder true and false comprises the steps:

(1) sample preparation: get lucidum spore powder medicinal material 10g to be identified, pulverize, it is for subsequent use to cross 80 mesh sieves;

(2) instrument: Fourier infrared spectrograph;

(3) blank scanning:

1. compressing tablet: precision takes by weighing dry potassium bromide fine powder 200mg and places agate mortar fully to be ground, with potassium bromide fine powder dislocation in the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, the potassium bromide blank of making, homogeneous transparent is answered in visual examination, without obvious particle;

2. scanning: the blank that presses is put into the scanning room scan, preserve spectrogram;

(4) standard items scanning:

1. compressing tablet: the 3mg lucidum spore powder standard items that precision is taken by weighing and the potassium bromide fine powder of the accurate 200mg drying that takes by weighing, place agate mortar fully to be ground, place the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, make the standard items sheet, visual examination should be even, without obvious particle;

2. scanning: the standard items sheet that presses is put into the scanning room scan, preserve spectrogram;

(5) Sample Scan:

1. compressing tablet: the sample that the 3mg step (1) that precision is taken by weighing obtains and the potassium bromide fine powder of the accurate 200mg drying that takes by weighing, place agate mortar fully to be ground, place the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, the sample strip of making, visual examination should be even, without obvious particle;

2. scanning: the sample strip that presses is put into the scanning room scan, preserve spectrogram;

(6) similarity is relatively:

1. sample spectrogram and standard items spectrogram are carried out similarity relatively;

2. criterion: the similarity of sample spectrogram and standard items spectrogram should be greater than 0.95.

Advantage of the present invention:

1, detect fast, the sample compressing tablet carries out infrared scan, carries out data analysis, and the process of haveing suffered only needs a few minutes;

2, sample nondestructive: sample size only needs 3 milligrams, and use amount is few;

3, information is comprehensive: sample carries out the all-wave Infrared spectrum scanning, and sample message is finished.

Description of drawings

Fig. 1 is the structured flowchart of FTIR spectrometer.

Fig. 2 is the comparison of lucidum spore powder infrared spectrogram to be measured and standard items.

Sample is more similar with the infrared spectrum peak shape of contrast lucidum spore powder among Fig. 2, standard items (figure a) with sample (figure b) at 3543cm ^-1And 3487cm ^-1Absorption peak strength basic identical.

Embodiment

The present invention is further illustrated below in conjunction with specific embodiment.

The lucidum spore powder standard items are purchased from: (Chinese biological drug inspection office 2008-1102)

Embodiment 1

A kind of method of the quick discriminating lucidum spore powder true and false comprises the steps:

(1) sample preparation: get lucidum spore powder medicinal material 10g to be identified, pulverize, it is for subsequent use to cross 80 mesh sieves;

(2) instrument: Fourier infrared spectrograph (FTIR) is by optical measuring system, computer data processing system, and the several major parts of computer interface and electronics systems form.Optical measuring system is used for the measurement collection data, and computing machine is used for deal with data and control instrument operation.Fig. 1 is the structured flowchart of Fourier infrared spectrograph;

(3) blank scanning:

1. compressing tablet: precision takes by weighing dry potassium bromide fine powder 200mg and places agate mortar fully to be ground, with potassium bromide fine powder dislocation in the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, the potassium bromide blank of making, homogeneous transparent is answered in visual examination, without obvious particle;

2. scanning: the blank that presses is put into the scanning room scan, preserve spectrogram;

(4) standard items scanning:

1. compressing tablet: the 3mg lucidum spore powder standard items that precision is taken by weighing and the potassium bromide fine powder of the accurate 200mg drying that takes by weighing, place agate mortar fully to be ground, place the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, make the standard items sheet, visual examination should be even, without obvious particle;

2. scanning: the standard items sheet that presses is put into the scanning room scan, preserve spectrogram;

(5) Sample Scan:

1. compressing tablet: the sample that the 3mg step (1) that precision is taken by weighing obtains and the potassium bromide fine powder of the accurate 200mg drying that takes by weighing, place agate mortar fully to be ground, place the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, the sample strip of making, visual examination should be even, without obvious particle;

2. scanning: the sample strip that presses is put into the scanning room scan, preserve spectrogram;

(6) similarity is relatively:

1. sample spectrogram and standard items spectrogram are carried out similarity relatively;

2. criterion: the similarity of sample spectrogram and standard items spectrogram should be greater than 0.95.See Fig. 2.

(7) result shows, lucidum spore powder medicinal material sample spectrogram to be identified and the similarity of standard items spectrogram should greater than 0.95, be genuine piece.

Analytical procedure

(1) spectrum transformation of coordinates

The infrared spectrum ordinate has two kinds of representations commonly used, i.e. transmissivity T and absorbance A.Application software can make mutually conversion between transmission spectrum and the absorption spectra easily.

(2) baseline correction

The spectrum that the spectrum that the FTIR spectrometer is got is digitizing, it is comprised of the absorbance of a series of data points.Therefore a spectrogram can be regarded as by the very little straight line of many spacings and be formed by connecting, and the data point of its spacing when carrying out FT determines.Baseline can be proofreaied and correct by some mathematical methods, can proofread and correct the baseline at each wave number place according to following equation:

$Y^{\′}\left(\stackrel{\‾}{v}\right)=Y\left(\stackrel{\‾}{v}\right)-(T+C\×\stackrel{\‾}{v})$

In the formula

It is wave number among the former figure Ordinate (absorbance) value at place,

It is the ordinate value after corresponding the correction.T is the numerical value relevant with baseline wander, and C is the numerical value relevant with this place's baseline inclination.If whole spectrum only need be done level correction, then C=0, T are constant; If only do slope correction, then T=0, C are constant; If do simultaneously level and slant correction, then T and C all are not 0; If this part spectrum is bending, then can reach by progressively changing T, C numerical value the purpose of correction.

The baseline of the infrared spectrum that records by experiment drifts about sometimes, tilts or be crooked, especially often can run into this situation in the spectrogram of solid sample preparation.The at first definite wave-number range that need proofread and correct of baseline correction program demonstrates 0 baseline position at display screen, adjusts parameter according to the spectrogram baseline that shows with keyboard by the user again and proofreaies and correct, until obtain till the satisfied result.

(3) normalization

After selected certain beam location, absorb the strongest absorbance with this beam location in the spectrogram and be as the criterion, other spectrogram is stretched, thereby make all spectrograms consistent in this position absorbance.

(4) related coefficient relatively

By using a large amount of mathematics filtrators, different chromatogram characteristics has just obtained strengthening or weakening.Except report related coefficient (correlaiton coefficient), also having a coefficient (factor) is that the average absorption intensity of expression spectrogram relatively is with the ratio of reference spectrogram.Can carry out related coefficient relatively with the reference spectrogram with a lot of spectrograms, thereby draw with the most similar spectrogram of reference spectrogram.

Spectrum resolution

1. characteristic absorption can only be used with reference spectrogram (standard items spectrogram), and they have shown lucidum spore powder and the relevant main absorption spectrum of goods thereof.The feature at some peak for example, the relation of they and adjacent peaks, or other zones of spectrum etc.

2. sample absorption spectrum should be compared from peak shape, peak position, peak intensity three aspects: and reference spectrum, to determine corresponding characteristic absorption relation, so as to the authenticity of judgement sample.

3. similarity analysis

(1) the similarity analysis instrument can provide clearly several signs that refer to specimen spectrogram and reference spectrogram (standard items spectrogram) similarity relation; Otherness so as to auxiliary judgment sample and reference

(2) obtain data statistic analysis result and conventional empirical analysis according to standard customization contained sample scope of phase, the similarity of test lucidum spore powder and reference standard sample; Occur more on the low sidely such as the result, should consider the authenticity of sample.

Claims (1)

1. a method of differentiating fast the lucidum spore powder true and false is characterized in that comprising the steps:

(1) sample preparation: get lucidum spore powder medicinal material 10g to be identified, pulverize, it is for subsequent use to cross 80 mesh sieves;

(2) instrument: Fourier infrared spectrograph;

(3) blank scanning:

1. compressing tablet: precision takes by weighing dry potassium bromide fine powder 200mg and places agate mortar fully to be ground, with potassium bromide fine powder dislocation in the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, the potassium bromide blank of making, homogeneous transparent is answered in visual examination, without obvious particle;

2. scanning: the blank that presses is put into the scanning room scan, preserve spectrogram;

(4) standard items scanning:

1. compressing tablet: the 3mg lucidum spore powder standard items that precision is taken by weighing and the potassium bromide fine powder of the accurate 200mg drying that takes by weighing, place agate mortar fully to be ground, place the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, make the standard items sheet, visual examination should be even, without obvious particle;

2. scanning: the standard items sheet that presses is put into the scanning room scan, preserve spectrogram;

(5) Sample Scan:

1. compressing tablet: the sample that the 3mg step (1) that precision is taken by weighing obtains and the potassium bromide fine powder of the accurate 200mg drying that takes by weighing, place agate mortar fully to be ground, place the press mold of diameter 13mm, shop cloth is even, be forced into 0.8Mpa, keep 1min, the sample strip of making, visual examination should be even, without obvious particle;

2. scanning: the sample strip that presses is put into the scanning room scan, preserve spectrogram;

(6) similarity is relatively:

1. sample spectrogram and standard items spectrogram are carried out similarity relatively;

2. criterion: the similarity of sample spectrogram and standard items spectrogram should be greater than 0.95.

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