CN103409318A

CN103409318A - Novel photoelectric capillary cell collector based on inclination optical fiber

Info

Publication number: CN103409318A
Application number: CN2013103928741A
Authority: CN
Inventors: 赵勇; 胡晟
Original assignee: Northeastern University China
Current assignee: Northeastern University China
Priority date: 2013-09-03
Filing date: 2013-09-03
Publication date: 2013-11-27
Anticipated expiration: 2033-09-03
Also published as: CN103409318B

Abstract

The invention provides a novel photoelectric capillary cell collector based on an inclination optical fiber. The novel photoelectric capillary cell collector is used for capturing and collecting biological cells and is characterized in that refraction spots provided by the inclination optical fiber irradiate on a hydrogenated amorphous silicon film on a lower semicircular indium tin oxide capillary glass, electric fields of upper and lower semicircular indium tin oxide capillary glasses are conducted at the same time to form a non-uniform electric field, the electric field enables cells to generate galvanic couple polarization, and the cells move towards the insides of a capillary glass tube under the control of the photoinduced dielectrophoresis force. The novel photoelectric capillary cell collector has the advantages of reasonable design, ingenious structure, low cost and long use distance; and with the adoption of the novel photoelectric capillary cell collector, the conductive indium tin oxide capillary glass and optical fiber materials are easily prepared and can resist corrosion, the inclination optical fiber photoelectric capillary cell collector is conveniently operated, and the needs of biologists of collecting the cells can be largely satisfied.

Description

A kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector

Technical field

The present invention is a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector, belongs to the biological microcontroller of photoelectricity field.

Background technology

The light-induction dielectrophoresis technology is biological micro-control technology (the document 1. P. Y. Chiou that a kind of photoelectricity of proposition in 2005 mutually combines, A. T. Ohta, M. C. Wu, " Massively parallel manipulation of single cells and microparticles using optical images; " Nature, vol. 436, pp. 370-372,2005.), it combines optical tweezers and micro-electrodynamic advantage, can high precision control single sensitive cells, simultaneously also can the larger area fast enriching micro-/as to receive particle, cell and protein.Therefore the light-induction dielectrophoresis biochip is gradually by a large amount of scholar's research and report (document 2. H. Hwang, J.-K. Park, " Optoelectrofluidic platforms for chemistry and biology; " Lab on a Chip, vol. 11, pp. 33-47,2011.).

In actual scientific research, for cell engineering experiment and biological life studies, the collection of cell is a requisite job.Permitted in the market eurypalynous cell harvestor, the improved medical cell collector that is 201020521893.1 as the patent No. has been used the U-shaped test tube with blind end to be made, and needs to use special chemical reagent; As the patent No., be a kind of cervix cast-off cells collector of 201020511664.1, its shortcoming is that range of application is narrower; As the patent No., be 200620134542.9 sieve formula colorectal cell collector, too loaded down with trivial details to the treatment scheme of cell, and need centrifugal treating, easily destroy the body activity of cell; As the patent No., be 200620087505.7 disposable esophageal cast-off cell collector, although simple to operate easy to use, also there is the shortcoming of the not reproducible utilization of resource.Therefore the invention discloses a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector, the hot spot of inducing that this instrument inclination angle optical fiber sends conducts upper and lower semicircle glass capillary, thereby makes cell generation polarization motion.Cell after polarization can be to the large regional movement of strength of electric field, and around the zone of inducing due to the optical fiber hot spot, strength of electric field is larger, so that cell moves to the facula position of glass capillary inside.Realize thus the collecting function of cell, the present invention is simple in structure, and easy to operate, can meet the needs of collecting tumour cell.

Summary of the invention

The technical problem that (one) will solve

Major Difficulties of the present invention: the one, on lower semi-circular tin indium oxide glass capillary inwall, carry out the plated film of amorphous silicon hydride; The 2nd, make the optical fiber with special angle fiber end face, and the encapsulation of this optical fiber and tin indium oxide glass capillary.

(2) technical scheme

The present invention is a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector.This collector is by upper and lower semicircle tin indium oxide glass capillary, inclination angle optical fiber, and fiber failure pen and plain conductor form.This cell harvestor provides light source and power supply by the fiber failure detecting pen, after inclination angle optical fiber sends and induces hot spot, hot spot is radiated at the electricity conducting that photo-generated carrier will occur lower semicircle tin indium oxide glass capillary, thereby with upper piece semicircle tin indium oxide glass capillary, form inhomogeneous field, can utilize thus light-induction dielectrophoresis power to realize the collection of cell.

In such scheme, described lower semi-circular tin indium oxide glass capillary utilizes the plasma enhanced chemical vapor deposition method, plates hydrogenation non crystal silicon film, makes it have light sensitive characteristic.

In such scheme, the upper and lower semicircle tin indium oxide glass capillary of described inclination angle optical fiber photoelectricity glass capillary is used ultraviolet glue bonding, guarantees upper and lower semicircle tin indium oxide glass capillary insulation, and is connected firmly.

In such scheme, described inclination angle optical fiber photoelectricity glass capillary, the smooth optical fiber of the end face corner cut that tilts is processed.Light refraction can occur in the optical fiber emergent light in cell culture fluid, therefore need to do inclined design to fiber end face, to guarantee that the complete uniform irradiation of optical fiber emergent light is to lower semicircle tin indium oxide glass capillary internal surface, the slant optical fiber angle that this invention is adopted is 45 °.

In such scheme, described inclination angle optical fiber photoelectricity glass capillary, 45 ° of inclination angle optical fiber are put into upper and lower semicircle tin indium oxide glass capillary.Put into process, also will guarantee that the slant optical fiber end face is towards upper piece semicircle tin indium oxide glass capillary, the fiber lengths of putting into is roughly 2/3 of tin indium oxide glass capillary length.

In such scheme, described cell harvestor, need will draw two plain conductors in fiber failure detecting pen positive-negative power, be connected on respectively on upper and lower semicircle tin indium oxide glass capillary, use the ultraviolet glue hydropexis, then with the insulation rubber muscle, the two ends metal wire is tightened.Optical fiber inserts fiber failure detecting pen Link Port, to be placed in cell culture fluid fully with the tin indium oxide glass capillary at optical fiber inclination angle, then open fiber failure detecting pen switch, cell will be subject to the effect of light-induction dielectrophoresis power and to tin indium oxide glass capillary internal motion.

(3) beneficial effect

From technique scheme, can find out, the present invention has following beneficial effect.

1) a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector of the present invention's design, the method that adopts electricity and optics to combine, only need fiber failure detecting pen built-in power that direct supply is provided, can reduce like this preparation technology's complicacy, have simultaneously reliability.

2) the present invention designs a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector, it is little that the glass capillary cell harvestor has a volume, the characteristics that handiness is high, and photoelectricity capillary cell collector is easy to operate, can meet the needs of tumour cell research fully.

The accompanying drawing explanation

Below each figure the inclination angle optical fiber of getting photoelectricity capillary cell collector structural parameter all with embodiment in identical.

Fig. 1 is inclination angle optical fiber electrocapillary cell harvestor structural representation.

Fig. 2 is based on finite element method for simulating photoelectricity kapillary collecting cell schematic diagram.

In Fig. 1: 1, first circular tin indium oxide glass capillary, 2, lower semi-circular tin indium oxide glass capillary, 3,45 ° of inclination angle optical fiber, 2-1, lower semi-circular tin indium oxide glass capillary, 2-2, hydrogenation non crystal silicon film, 3-1, fibre cladding, 3-2, fiber core.

In Fig. 2: 4,45 ° of inclination angle optical fiber, 5, upper and lower semicircle tin indium oxide glass capillary, 6, cell cultures solution, 7, cell.

Embodiment

For making the purpose, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, and with reference to accompanying drawing, concrete structure of the present invention is described in further detail.

A kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector of the present invention, be illustrated in figure 1 upper and lower semicircle tin indium oxide glass capillary and 45 ° of inclination angle optical fiber combination structural representations.Lower semi-circular tin indium oxide glass capillary (2-1) radius is 0.07mm, pipe range 100mm, by the plasma enhanced chemical vapor deposition method, plated hydrogenated amorphous silicon material (2-2), made lower semi-circular tin indium oxide glass capillary (2) possess light sensitive characteristic; Ultraviolet glue is spread upon to upper and lower semicircle tin indium oxide glass capillary junction, make upper and lower semicircle tin indium oxide glass capillary be connected firmly and there is no gap; The optical fiber of level and smooth end face carries out meticulous polishing, makes the optical fiber face that inclines reach 45 degree oblique angles (3); Optical fiber is inserted in the tin indium oxide glass capillary (5) after binding, and guarantee that the slant optical fiber end face is towards first circular tin indium oxide glass capillary pipe face, re-use ultraviolet glue and spread upon the place, gap that optical fiber inserts, fixed in tin indium oxide kapillary inside to guarantee optical fiber, and be not subjected to the interference of mechanical vibration; Positive and negative two-way metal wire is drawn in fiber failure detecting pen power supply inside, is connected to respectively upper and lower semicircle tin indium oxide glass capillary two ends, and with the ultraviolet glue water dungeon by fixing; Optical fiber inserts fiber failure detecting pen Link Port place; To be placed in cell culture fluid with the tin indium oxide glass capillary at optical fiber inclination angle, and then open the fiber failure detecting pen, cell will be subject to the effect of light-induction dielectrophoresis power and to the kapillary internal motion.This instrument being captured and collecting for biomass cells.The photoelectricity glass capillary is placed in to cell culture fluid (6), make the optical fiber hot spot induce the hydrogenated amorphous silicon material of lower semi-circular tin indium oxide capillary glass surface to conduct, the electrical forces of upper and lower semicircle tin indium oxide glass capillary can force cell (7) to move to tube interior.

Fig. 2 (a) and the cell (b) obtained for finite element business software COMSOL 4.3a emulation are subjected to light-induction dielectrophoresis power motion diagram, the original state time, cell be randomly dispersed in pipe under 80 μ m places, as shown in Fig. 2 (a).After the light source hot spot of fiber failure detecting pen caused inhomogeneous field, cell was subjected to dielectrophoretic force that the motion near pipe occurs gradually.In analysis of finite element method, in formed photoelectricity kapillary photoinduction electric field, cell is subjected to the stokes pulling force in light-induction dielectrophoresis power and cell movement to move to gradually the apical position that the pipe electric field is stronger, as shown in Fig. 2 (b).The cell emulation experiment of this Finite Element Method has also better proved the ability of photoelectricity glass capillary collecting cell.

Claims

1. the capillary cell of the novel photoelectric based on an inclination angle optical fiber collector, be by upper and lower semicircle tin indium oxide glass capillary, inclination optical fiber, and the fiber failure detecting pen combines.

2. it is characterized in that the hot spot that inclination angle optical fiber sends, shine the hydrogenation non crystal silicon film (2-2) on lower semi-circular tin indium oxide glass capillary (2-1), meanwhile upper and lower semicircle tin indium oxide glass capillary electric field conducting, and formed inhomogeneous field, make the polarization of cell generation galvanic couple, cell is subjected to the control of light-induction dielectrophoresis power and moves in glass capillary.

3. a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector as claimed in claim 1, it is characterized in that: the radius r=0.05mm of lower semi-circular tin indium oxide glass capillary (2-1), long L=100mm, and should use the plasma enhanced chemical vapor deposition method to plate hydrogenation non crystal silicon film (2-2) in surface, and make its surface have light sensitive characteristic; Upper and lower semicircle tin indium oxide glass capillary ultraviolet glue hydropexis.

4. a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector as claimed in claim 1 is characterized in that: level and smooth end face fibre diameter is 125 μ m, and it is polished and makes the optical fiber face that inclines reach 45 ° of oblique angles (3); Inclination angle optical fiber (3) inserts tin indium oxide glass capillary 2L/3 place, and the inclined end face that guarantees optical fiber is towards first circular tin indium oxide glass capillary (1), the hydropexis of inclination angle optical fiber (3) use ultraviolet glue.

5. a kind of capillary cell of novel photoelectric based on inclination angle optical fiber collector as claimed in claim 3, it is characterized in that: positive and negative two-way metal wire is drawn in fiber failure detecting pen power supply inside, be connected to respectively on upper and lower semicircle tin indium oxide glass capillary outer wall, with ultraviolet glue and insulation rubber muscle fixed metal electrode; Fiber failure detecting pen semicircle tin indium oxide glass capillary up and down provides bias voltage, to the inclination angle optical fiber in the tin indium oxide glass capillary, provides and induces light source simultaneously.

6. through Finite Element Method, confirm, the cell harvesting pipe is placed in cell culture fluid, and 80 μ m places can complete the collection of cell under it.