CN103409318B - Novel photoelectric capillary cell collector based on inclination optical fiber - Google Patents
Novel photoelectric capillary cell collector based on inclination optical fiber Download PDFInfo
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- CN103409318B CN103409318B CN201310392874.1A CN201310392874A CN103409318B CN 103409318 B CN103409318 B CN 103409318B CN 201310392874 A CN201310392874 A CN 201310392874A CN 103409318 B CN103409318 B CN 103409318B
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Abstract
The invention provides a novel photoelectric capillary cell collector based on an inclination optical fiber. The novel photoelectric capillary cell collector is used for capturing and collecting biological cells and is characterized in that refraction spots provided by the inclination optical fiber irradiate on a hydrogenated amorphous silicon film on a lower semicircular indium tin oxide capillary glass, electric fields of upper and lower semicircular indium tin oxide capillary glasses are conducted at the same time to form a non-uniform electric field, the electric field enables cells to generate galvanic couple polarization, and the cells move towards the insides of a capillary glass tube under the control of the photoinduced dielectrophoresis force. The novel photoelectric capillary cell collector has the advantages of reasonable design, ingenious structure, low cost and long use distance; and with the adoption of the novel photoelectric capillary cell collector, the conductive indium tin oxide capillary glass and optical fiber materials are easily prepared and can resist corrosion, the inclination optical fiber photoelectric capillary cell collector is conveniently operated, and the needs of biologists of collecting the cells can be largely satisfied.
Description
Technical field
The present invention is a kind of novel photoelectric capillary cell collector based on inclination angle optical fiber, belongs to the biological microcontroller of photoelectricity field.
Background technology
Light-induction dielectrophoresis technology is biological micro-control technology (the document 1. P. Y. Chiou that a kind of photoelectricity of proposition in 2005 mutually combines, A. T. Ohta, M. C. Wu, " Massively parallel manipulation of single cells and microparticles using optical images; " Nature, vol. 436, pp. 370-372,2005.), it combines optical tweezers and micro-electrodynamic advantage, can high precision control single sensitive cells, simultaneously also can larger area fast enriching micro-/to receive particle, cell and protein.Therefore light-induction dielectrophoresis biochip is gradually by a large amount of scholar's research and report (document 2. H. Hwang, J.-K. Park, " Optoelectrofluidic platforms for chemistry and biology; " Lab on a Chip, vol. 11, pp. 33-47,2011.).
In actual scientific research, for cell engineering experiment and biological life studies, the collection of cell is a requisite job.Permitted in the market eurypalynous cell harvestor, the improved medical cell collector that is 201020521893.1 as the patent No. has been used the U-shaped test tube with blind end to be made, and need to use special chemical reagent; As a kind of cervix cast-off cells collector that the patent No. is 201020511664.1, its shortcoming is that range of application is narrower; As the sieve formula colorectal cell collector that the patent No. is 200620134542.9, too loaded down with trivial details to the treatment scheme of cell, and need centrifugal treating, the body that easily destroys cell is active; As the patent No. disposable esophageal cast-off cell collector that is 200620087505.7, although simple to operate easy to use, also there is the shortcoming of the not reproducible utilization of resource.Therefore the invention discloses a kind of novel photoelectric capillary cell collector based on inclination angle optical fiber, the induction hot spot that this instrument inclination angle optical fiber sends conducts upper and lower semicircle glass capillary, thereby makes cell generation polarization motion.Cell after polarization can be to the large regional movement of strength of electric field, and due to the region of optical fiber hot spot induction, around strength of electric field is larger, so that cell moves to the facula position of glass capillary inside.The collecting function of realizing thus cell, the present invention is simple in structure, and easy to operate, can meet the needs of collecting tumour cell.
Summary of the invention
(1) technical problem that will solve
Major Difficulties of the present invention: the one, on lower semi-circular tin indium oxide glass capillary inwall, carry out the plated film of amorphous silicon hydride; The 2nd, make the optical fiber with special angle fiber end face, and the encapsulation of this optical fiber and tin indium oxide glass capillary.
(2) technical scheme
The present invention is a kind of novel photoelectric capillary cell collector based on inclination angle optical fiber.This collector is by upper and lower semicircle tin indium oxide glass capillary, inclination angle optical fiber, and fiber failure pen and plain conductor form.This cell harvestor provides light source and power supply by fiber failure detecting pen, when inclination angle optical fiber sends after induction hot spot, hot spot is radiated at lower semicircle tin indium oxide glass capillary and will the electricity conducting of photo-generated carrier occurs, thereby form inhomogeneous field with upper piece semicircle tin indium oxide glass capillary, can utilize thus light-induction dielectrophoresis power to realize the collection of cell.
In such scheme, described lower semi-circular tin indium oxide glass capillary utilizes plasma enhanced chemical vapor deposition method, plates hydrogenation non crystal silicon film, makes it have light sensitive characteristic.
In such scheme, the upper and lower semicircle tin indium oxide glass capillary of described inclination angle optical fiber photoelectricity glass capillary is used ultraviolet glue bonding, guarantees upper and lower semicircle tin indium oxide glass capillary insulation, and is connected firmly.
In such scheme, described inclination angle optical fiber photoelectricity glass capillary, the smooth optical fiber of the end face corner cut that tilts is processed.In cell culture fluid, can there is light refraction in optical fiber emergent light, therefore need to do inclined design to fiber end face, to guarantee that the complete uniform irradiation of optical fiber emergent light is to lower semicircle tin indium oxide glass capillary internal surface, the slant optical fiber angle that this invention adopts is 45 °.
In such scheme, described inclination angle optical fiber photoelectricity glass capillary, 45 ° of inclination angle optical fiber are put into upper and lower semicircle tin indium oxide glass capillary.Put into process, also will guarantee that slant optical fiber end face is towards upper piece semicircle tin indium oxide glass capillary, the fiber lengths of putting into is roughly 2/3 of tin indium oxide glass capillary length.
In such scheme, described cell harvestor, need will draw two plain conductors in fiber failure detecting pen positive-negative power, be connected on respectively on upper and lower semicircle tin indium oxide glass capillary, use ultraviolet glue hydropexis, then with insulation rubber muscle, two ends metal wire is tightened.Optical fiber inserts fiber failure detecting pen Link Port, tin indium oxide glass capillary with optical fiber inclination angle is placed in to cell culture fluid completely, then open fiber failure detecting pen switch, cell will be subject to the effect of light-induction dielectrophoresis power and to tin indium oxide glass capillary internal motion.
(3) beneficial effect
From technique scheme, can find out, the present invention has following beneficial effect.
1) a kind of novel photoelectric capillary cell collector based on inclination angle optical fiber of the present invention's design, the method that adopts electricity and optics to combine, only need fiber failure detecting pen built-in power that direct supply is provided, can reduce like this preparation technology's complicacy, there is reliability simultaneously.
2) the present invention designs a kind of novel photoelectric capillary cell collector based on inclination angle optical fiber, it is little that glass capillary cell harvestor has volume, the feature that handiness is high, and photoelectricity capillary cell collector is easy to operate, can meet the needs of tumour cell research completely.
Accompanying drawing explanation
The structural parameter of photoelectricity capillary cell collector that following figure gets inclination angle optical fiber are all identical with embodiment.
Fig. 1 is inclination angle optical fiber electrocapillary cell harvestor structural representation.
Fig. 2 is based on finite element method for simulating photoelectricity kapillary collecting cell schematic diagram.
In Fig. 1: 1, first circular tin indium oxide glass capillary, 2, lower semi-circular tin indium oxide glass capillary, 3,45 ° of inclination angle optical fiber, 2-1, lower semi-circular tin indium oxide glass capillary, 2-2, hydrogenation non crystal silicon film, 3-1, fibre cladding, 3-2, fiber core.
In Fig. 2: 4,45 ° of inclination angle optical fiber, 5, upper and lower semicircle tin indium oxide glass capillary, 6, cell cultures solution, 7, cell.
Embodiment
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, and with reference to accompanying drawing, concrete structure of the present invention is described in further detail.
A kind of novel photoelectric capillary cell collector based on inclination angle optical fiber of the present invention, is illustrated in figure 1 upper and lower semicircle tin indium oxide glass capillary and 45 ° of inclination angle optical fiber combination structural representations.Lower semi-circular tin indium oxide glass capillary (2-1) radius is 0.07mm, pipe range 100mm, by plasma enhanced chemical vapor deposition method, plated hydrogenated amorphous silicon material (2-2), made lower semi-circular tin indium oxide glass capillary (2) possess light sensitive characteristic; Ultraviolet glue is spread upon to upper and lower semicircle tin indium oxide glass capillary junction, make upper and lower semicircle tin indium oxide glass capillary be connected firmly and there is no gap; The optical fiber of level and smooth end face carries out meticulous polishing, makes the optical fiber face that inclines reach 45 degree oblique angles (3); Optical fiber is inserted in the tin indium oxide glass capillary (5) after binding, and guarantee that slant optical fiber end face is towards first circular tin indium oxide glass capillary pipe face, re-use ultraviolet glue and spread upon the place, gap that optical fiber inserts, fixed in tin indium oxide kapillary inside to guarantee optical fiber, and be not subject to the interference of mechanical vibration; Positive and negative two-way metal wire is drawn in fiber failure detecting pen power supply inside, is connected to respectively upper and lower semicircle tin indium oxide glass capillary two ends, and with ultraviolet glue water dungeon by fixing; Optical fiber inserts fiber failure detecting pen Link Port place; Tin indium oxide glass capillary with optical fiber inclination angle is placed in to cell culture fluid, then opens fiber failure detecting pen, cell will be subject to the effect of light-induction dielectrophoresis power and to kapillary internal motion.This instrument being captured and collecting for biomass cells.Photoelectricity glass capillary is placed in to cell culture fluid (6), the hydrogenated amorphous silicon material of optical fiber hot spot induction lower semi-circular tin indium oxide capillary glass surface is conducted, and the electrical forces of upper and lower semicircle tin indium oxide glass capillary can force cell (7) to move to tube interior.
Fig. 2 (a) and the cell (b) obtaining for finite element business software COMSOL 4.3a emulation are subject to light-induction dielectrophoresis power motion diagram, the original state time, cell be randomly dispersed in pipe under 80 μ m places, as shown in Fig. 2 (a).When the light source hot spot of fiber failure detecting pen causes after inhomogeneous field, cell is subject to dielectrophoretic force that the motion near pipe occurs gradually.In analysis of finite element method, in formed photoelectricity kapillary photoinduction electric field, cell is subject to the stokes pulling force in light-induction dielectrophoresis power and cell movement to move to gradually the apical position that pipe electric field is stronger, as shown in Fig. 2 (b).The cell emulation experiment of this Finite Element Method has also better proved the ability of photoelectricity glass capillary collecting cell.
Claims (1)
1. the novel photoelectric capillary cell collector based on inclination angle optical fiber, is characterized in that: by upper and lower semicircle tin indium oxide glass capillary, and inclination angle optical fiber, and fiber failure detecting pen combines; The hot spot that inclination angle optical fiber sends, irradiates the hydrogenation non crystal silicon film on lower semi-circular tin indium oxide glass capillary, the radius of lower semi-circular tin indium oxide glass capillary
r=0.07mm, long
l=100mm, hydrogenation non crystal silicon film using plasma strengthens chemical Vapor deposition process and is plated on the surface of lower semi-circular tin indium oxide glass capillary, makes lower semi-circular tin indium oxide glass capillary surface have light sensitive characteristic; Upper and lower semicircle tin indium oxide glass capillary ultraviolet glue hydropexis, guarantees upper and lower semicircle tin indium oxide glass capillary insulation and is connected firmly; Inclination angle fibre diameter is 125 μ m, and the face oblique angle that inclines is 45 °; Inclination angle optical fiber inserts tin indium oxide glass capillary length 2/3 position, and the inclination angle end face that guarantees inclination angle optical fiber is towards first circular tin indium oxide glass capillary; Positive and negative two-way metal wire is drawn in fiber failure detecting pen power supply inside, is connected to respectively on upper and lower semicircle tin indium oxide glass capillary outer wall, with ultraviolet glue and insulation rubber muscle fixed metal electrode; Fiber failure detecting pen up and down semicircle tin indium oxide glass capillary provides bias voltage, induction light source is provided to the inclination angle optical fiber in tin indium oxide glass capillary simultaneously, the conducting of upper and lower semicircle tin indium oxide glass capillary electric field, form inhomogeneous field, make the polarization of cell generation galvanic couple, cell is moved by the control of light-induction dielectrophoresis power in glass capillary.
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| CN103409318B true CN103409318B (en) | 2014-08-20 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102013019889B4 (en) * | 2013-11-28 | 2015-07-30 | Airbus Defence and Space GmbH | Photobioreactor with mats of light-outcoupling optical fibers and an electric traveling field generating electrically conductive fibers |
| CN104371917A (en) * | 2014-09-16 | 2015-02-25 | 东北大学 | New method for inducing chain forming of cells based on double-photoelectrical film biochip |
| CN104974997B (en) * | 2015-05-28 | 2018-07-31 | 东北大学 | A kind of cell novel method for separating using parallel electric field formula photoelectric chip |
| CN106512478B (en) * | 2016-11-28 | 2018-12-28 | 延边大学 | The controllable capillary size exclusion chromatography separation method of electric field |
| CN112280649B (en) * | 2020-10-16 | 2022-05-10 | 南开大学 | Cell sorter based on inclined fiber bragg grating optical tweezers |
| CN113376230B (en) * | 2021-06-10 | 2022-09-06 | 福州大学 | Photoelectrochemical optical fiber microelectrode adopting electrode internal illumination mode and preparation method thereof |
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Patent Citations (4)
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| CN1526024A (en) * | 2001-02-07 | 2004-09-01 | ��ʡ��ѧԺ | Optoelectronic detection system |
| CN1588088A (en) * | 2004-09-23 | 2005-03-02 | 清华大学 | Micro flow control chip detecting system for flowing cell detection |
| CN102628758A (en) * | 2012-03-01 | 2012-08-08 | 麦克奥迪实业集团有限公司 | Collection device for collecting cells after laser microdissection, method and system thereof |
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Non-Patent Citations (4)
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