CN103404382A - Identification method of photoperiod sensibility quantization of wheat - Google Patents
Identification method of photoperiod sensibility quantization of wheat Download PDFInfo
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- CN103404382A CN103404382A CN2013103326893A CN201310332689A CN103404382A CN 103404382 A CN103404382 A CN 103404382A CN 2013103326893 A CN2013103326893 A CN 2013103326893A CN 201310332689 A CN201310332689 A CN 201310332689A CN 103404382 A CN103404382 A CN 103404382A
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Abstract
The invention discloses an identification method of photoperiod sensibility quantization of wheat. Mainly aiming at Huanghuai winter wheat areas, the identification method of the photoperiod sensibility quantization of the wheat includes: planting a wheat material to be identified and a comparison material in different periods, wherein the planting periods include an early planting period and a late planting period at an interval of 40 days, and the last planting period ends before the end of November; performing regular farming management to avoid soil drought and fertilizer deficiency; accurately recording heading periods of every material in the early and the late planting periods; calculating difference between the heading periods of every material in the early and the late planting periods, and normally the difference is 1-20 days. The larger the difference between the heading periods is, the stronger the photoperiod sensibility of the material is, the smaller the difference between the heading periods is, the weaker the photoperiod sensibility of the material is, and thus the photoperiod sensibility of the wheat can be quantitatively evaluated. In addition, aiming at the photoperiod sensibility, phenotypic functional identification is performed directly, the photoperiod sensibility can be quantitatively identified, and the identification method is simple, convenient, fast and efficient in technology.
Description
Technical field
The invention belongs to grain crops studying technological domain, relate in particular to the authentication method (mainly for varieties in Huang-Huai-Hai Plain) that a grow wheat photoperiod sensitivity quantizes.
Background technology
Vernalization characteristic and photoperiod sensitivity are determining the developmental process of wheat, and being affects the adaptive important physiological property of wheat, are also the current and following crucial research contents that breaks through the wheat yield bottleneck by developmental regulation.Photoperiod sensitivity is a kind of complex physiologic proterties of wheat breed, by a plurality of genes, controlled, and be the controlled factor of Growth of Wheat.The effect of photoperiod sensitivity is not merely long-day energy accelerated development, should make in addition a certain duration of day node function corresponding with the specific developmental stage of plant, and this is also the phenophase to keep stable heredity and physiological foundation; If a wheat breed does not possess photoperiod sensitivity, temperature (or accumulated temperature) will become the deciding factor of its developmental process of left and right so.Understand the photoperiod sensitivity of wheat, which type of environmental condition can know that it grows needs; Also be convenient to for the suitable wheat breed of specific region seed selection.
Current, identify that the conventional method of wheat photoperiod sensitivity is very complicated loaded down with trivial details, need to set a plurality of temperature and illumination condition and observe in detail its development progress, appraisal cost is high; In addition, also useful molecule labelling method carries out the photoperiod genotype identification at present, thereby the photoperiod sensitivity of judgement wheat breed, but due to photoperiod sensitivity, be the complex character of being controlled by multiple gene (some gene is not yet verified), and with the vernalization gene, interaction is arranged, add existing molecular labeling very limited, so the accuracy of identifying is very low.Thereby the authentication method quantized in the urgent need to developing a kind of quick, easy photoperiod sensitivity, the present invention namely utilizes the above-mentioned background principle to develop for this target.
Summary of the invention
The authentication method that provides a grow wheat photoperiod sensitivity to quantize is provided the purpose of the embodiment of the present invention, being intended to solve the complicated loaded down with trivial details and molecular marker identification method accuracy of traditional authentication method, very low (photoperiod sensitivity is the complex character by controlled by multiple genes, and with the vernalization gene, interaction is arranged, existing molecular labeling is very limited) problem.
The embodiment of the present invention is achieved in that the authentication method that a grow wheat photoperiod sensitivity quantizes, and the authentication method that this wheat photoperiod sensitivity quantizes comprises the following steps:
Step 1, sooner or later two dates of seeding, 40 days, interval, plant wheat lines and control material to be identified, before by the end of November, completes last date of seeding;
Step 2, conventional farming management, avoid soil drought or fertilizer deficiency;
Step 3, each material of precise record is the heading stage of two dates of seeding sooner or later;
Step 4, calculate each material difference at heading stage between date of seeding sooner or later, and difference is 1-20 days.
Further, in step 1, choose the Yellow River-Huai River region photoperiod sensitivity by by force to weak control material be followed successively by little lay down No. 6, all wheats 18, littlely lay down 22, Yanzhan4110 and Xi Nong 1376.
Further, the authentication method that this wheat photoperiod sensitivity quantizes shows: between date of seeding difference is less heading stage sooner or later, show that this material is more responsive for photoperiodic reaction, difference shows that more this material is more insensitive for photoperiodic reaction, but the photoperiod sensitivity of quantitatively evaluating wheat like this.
The authentication method that wheat photoperiod sensitivity provided by the invention quantizes by directly for photoperiod sensitivity, carrying out the phenotypic function evaluation, can quantize to identify to photoperiod sensitivity that technology is easy, rapidly and efficiently.The present invention can accurate easy Screening and Identification wheat lines photoperiod sensitivity; For wheat breed Adaptability Evaluation and kind layout provide foundation; In conjunction with the photoperiod genotype identification, can be for heredity and molecular biology research.In addition, the inventive method is simple, easy to operate, the authentication method that provides a kind of good wheat photoperiod sensitivity to quantize.
The accompanying drawing explanation
Fig. 1 is the flow chart of the authentication method that quantizes of the wheat photoperiod sensitivity that provides of the embodiment of the present invention.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Fig. 1 shows the authentication method flow process that wheat photoperiod sensitivity provided by the invention quantizes.For convenience of explanation, only show part related to the present invention.
The authentication method that the wheat photoperiod sensitivity of the embodiment of the present invention quantizes, the authentication method that this wheat photoperiod sensitivity quantizes comprises the following steps:
Step 1, sooner or later two dates of seeding, 40 days, interval, plant wheat lines and control material to be identified, before by the end of November, completes last date of seeding;
Step 2, conventional farming management, avoid soil drought or fertilizer deficiency;
Step 3, precise record material to be identified is the heading stage of two dates of seeding sooner or later;
Step 4, calculate each material difference at heading stage between date of seeding sooner or later, and difference is 1-20 days.
As a prioritization scheme of the embodiment of the present invention, in step 1, choose the Yellow River-Huai River region photoperiod sensitivity by by force to weak control material be followed successively by little lay down No. 6, all wheats 18, littlely lay down 22, Yanzhan4110 and Xi Nong 1376.
A prioritization scheme as the embodiment of the present invention, the authentication method that this wheat photoperiod sensitivity quantizes shows: between date of seeding difference is less heading stage sooner or later, show that this material is more responsive for photoperiodic reaction, difference shows that more this material is more insensitive for photoperiodic reaction, but the photoperiod sensitivity of quantitatively evaluating wheat like this.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, the authentication method of the wheat photoperiod sensitivity of embodiment of the present invention quantification comprises the following steps:
S101: sooner or later two dates of seeding, 40 days, interval, plant wheat lines and control material to be identified, before by the end of November, completes last date of seeding;
S102: conventional farming management, avoid soil drought or fertilizer deficiency;
S103: precise record material to be identified is the heading stage of two dates of seeding sooner or later;
S104: calculate each material difference at heading stage between date of seeding sooner or later, difference is 1-20 days.
Concrete steps of the present invention are:
The first step, treat expert evidence and control material, the Yellow River-Huai River region photoperiod sensitivity by strong to weak control material be followed successively by little lay down No. 6 (by force), all wheat 18(are stronger), in the little 22(of laying down), Yanzhan4110 () is and if western agriculture 1376(), carry out interval sowing, two dates of seeding of morning and evening, early sowing is generally normal sowing dates (basically identical with the production date of seeding), late sowing and early sowing interval 40 days, before by the end of November, complete last date of seeding, can guarantee that so material to be identified all can pass through vernalization stage;
Second step, conventional farming management, avoid soil drought or fertilizer deficiency;
At precise record material to be identified and control material heading stage of two dates of seeding sooner or later after the 3rd step, spring wheat jointing, be the important phenology index of reflection developmental process heading stage;
The 4th step, calculate each material difference at heading stage between date of seeding sooner or later, and difference is generally 1-20 days;
Reach a conclusion: sooner or later between date of seeding difference is less heading stage, shows that this material is more responsive for photoperiodic reaction, and difference shows that more this material is more insensitive for photoperiodic reaction, but photoperiod sensitivity of quantitatively evaluating wheat like this.With the difference at heading stage of control material, comparing the light sensitivity that can treat more intuitively expert evidence sorts out.The preferred parameter of recommending is in Table 1:
Table 1: the preferred parameter that the wheat photoperiod sensitivity is identified
Below in conjunction with embodiment, technical scheme of the present invention is described in more detail.
Expert evidence is: the western agriculture 979(of wheat breed identifies place: Yangling Shaanxi)
Step 1, to western agriculture 979 and control material [the Yellow River-Huai River region photoperiod sensitivity by strong to weak control material be followed successively by little lay down No. 6 (by force), all wheat 18(are stronger), in the little 22(of laying down), Yanzhan4110 () is and if western agriculture 1376()] carry out interval sowing.Sooner or later two dates of seeding,, early sowing is October 3, and late sowing was November 12;
Step 2, conventional farming management, avoid soil drought or fertilizer deficiency;
At precise record material to be identified and control material heading stage of two dates of seeding sooner or later after step 3, spring wheat jointing, be the important phenology index of reflection developmental process heading stage;
Step 4, calculate each material difference at heading stage between date of seeding sooner or later: western agriculture 979 early late sowing difference at heading stage is 4, little laying down No. 6 is 1, all wheats 18 are 3, little laying down 22 is that 5 days, Yanzhan4110 are 10, western agriculture 1376 is 14, can judge that thus western agriculture 979 photoperiod sensitivities belong to middle strongly-typed, between week 18 and little laying down between 22.
Step 5, sooner or later between date of seeding difference is less heading stage, shows that this material is more responsive for photoperiodic reaction, and difference shows that more this material is more insensitive for photoperiodic reaction, but photoperiod sensitivity of quantitatively evaluating wheat like this.With the difference at heading stage of control material, comparing the light sensitivity that can treat more intuitively expert evidence sorts out.
Beneficial effect after the present invention identifies:
1. the photoperiod sensitivity that accurately identifies easily the western agriculture 979 of wheat lines is middle strongly-typed, between all wheats 18 and little laying down between 22;
2. for wheat breed Adaptability Evaluation and kind layout, provide foundation, according to this qualification result, optimized Regional Distribution and the cultivation scheme of western agriculture 979;
3. the method, in conjunction with the photoperiod genotype identification, helps phenogenesis research and the molecular biology research of western agriculture 979.
The present invention can accurate easy Screening and Identification wheat lines photoperiod sensitivity; For wheat breed Adaptability Evaluation and kind layout provide foundation.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. the authentication method of a grow wheat photoperiod sensitivity quantification, is characterized in that, the authentication method that this wheat photoperiod sensitivity quantizes comprises the following steps:
Step 1, sooner or later two dates of seeding, 40 days, interval, plant wheat lines and control material to be identified, before by the end of November, completes last date of seeding;
Step 2, conventional farming management, avoid soil drought or fertilizer deficiency;
Step 3, put down in writing each material heading stage of two dates of seeding sooner or later;
Step 4, calculate each material difference at heading stage between date of seeding sooner or later, and difference is 1-20 days.
2. the authentication method that quantizes of wheat photoperiod sensitivity as claimed in claim 1, it is characterized in that, in step 1, expert evidence and control material choose the Yellow River-Huai River region photoperiod sensitivity by by force to weak control material be followed successively by little lay down No. 6, all wheats 18, littlely lay down 22, Yanzhan4110 and Xi Nong 1376.
3. the authentication method of wheat photoperiod sensitivity quantification as claimed in claim 1, is characterized in that, the authentication method that this wheat photoperiod sensitivity quantizes shows:
Sooner or later between date of seeding difference is less heading stage, shows that this material is stronger for photoperiodic reaction susceptibility, and difference shows that more this material is more weak for photoperiodic reaction susceptibility, but photoperiod sensitivity of quantitatively evaluating wheat like this.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165174A (en) * | 2015-08-03 | 2015-12-23 | 山东省农业科学院作物研究所 | Method for detection of wheat springness and winterness and photoperiod characteristics |
| CN106900389A (en) * | 2017-03-10 | 2017-06-30 | 西北农林科技大学 | A kind of method for identifying Growth of Wheat stability |
| CN111034558A (en) * | 2019-12-19 | 2020-04-21 | 扬州大学 | Method for field identification and evaluation of late sowing resistance and early maturity of wheat variety |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165174A (en) * | 2015-08-03 | 2015-12-23 | 山东省农业科学院作物研究所 | Method for detection of wheat springness and winterness and photoperiod characteristics |
| CN105165174B (en) * | 2015-08-03 | 2018-01-26 | 山东省农业科学院作物研究所 | A kind of method for detecting wheat Growth habit and photoperiod characteristic |
| CN106900389A (en) * | 2017-03-10 | 2017-06-30 | 西北农林科技大学 | A kind of method for identifying Growth of Wheat stability |
| CN111034558A (en) * | 2019-12-19 | 2020-04-21 | 扬州大学 | Method for field identification and evaluation of late sowing resistance and early maturity of wheat variety |
| CN111034558B (en) * | 2019-12-19 | 2022-09-16 | 扬州大学 | Method for field identification and evaluation of late sowing resistance and early maturity of wheat variety |
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Application publication date: 20131127 |