CN103403180A - 细胞的表征 - Google Patents

细胞的表征 Download PDF

Info

Publication number
CN103403180A
CN103403180A CN2011800509567A CN201180050956A CN103403180A CN 103403180 A CN103403180 A CN 103403180A CN 2011800509567 A CN2011800509567 A CN 2011800509567A CN 201180050956 A CN201180050956 A CN 201180050956A CN 103403180 A CN103403180 A CN 103403180A
Authority
CN
China
Prior art keywords
cell
coding rna
cells
spectrum
quality
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011800509567A
Other languages
English (en)
Chinese (zh)
Inventor
V·奥布里恩
C·希利尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sistemic Scotland Ltd
Original Assignee
Sistemic Scotland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sistemic Scotland Ltd filed Critical Sistemic Scotland Ltd
Publication of CN103403180A publication Critical patent/CN103403180A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800509567A 2010-08-23 2011-08-19 细胞的表征 Pending CN103403180A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1014049.9 2010-08-23
GBGB1014049.9A GB201014049D0 (en) 2010-08-23 2010-08-23 Cell characterisation
PCT/GB2011/001241 WO2012025709A1 (en) 2010-08-23 2011-08-19 Cell characterisation

Publications (1)

Publication Number Publication Date
CN103403180A true CN103403180A (zh) 2013-11-20

Family

ID=42984495

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800509567A Pending CN103403180A (zh) 2010-08-23 2011-08-19 细胞的表征

Country Status (12)

Country Link
US (4) US20130196875A1 (enExample)
EP (1) EP2609217B1 (enExample)
JP (1) JP2013535979A (enExample)
KR (1) KR20140006775A (enExample)
CN (1) CN103403180A (enExample)
AU (1) AU2011294936A1 (enExample)
BR (1) BR112013004202A2 (enExample)
CA (1) CA2847151C (enExample)
GB (1) GB201014049D0 (enExample)
IL (1) IL224888B (enExample)
SG (2) SG10201506595QA (enExample)
WO (1) WO2012025709A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113658636A (zh) * 2021-07-22 2021-11-16 未来智人再生医学研究院(广州)有限公司 一种评估多能干细胞质量的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2859119A2 (en) * 2012-06-12 2015-04-15 Institute for Systems Biology Complex rna composition of bodily fluids
WO2017160880A1 (en) * 2016-03-14 2017-09-21 Aelan Cell Technologies, Inc. Compositions and methods for the quality control of stem cell preparations
SG11201809902QA (en) 2016-05-09 2018-12-28 Sistemic Scotland Ltd Functional prediction of cellular functions by means of microrna expression profiling in mesenchymal stem cells
JP6629770B2 (ja) * 2017-01-19 2020-01-15 シスメックス株式会社 細胞の分化状態を評価する方法
JP6918062B2 (ja) * 2017-01-19 2021-08-11 シスメックス株式会社 細胞の分化状態を評価する方法
GB2570404A (en) * 2018-01-22 2019-07-24 Sistemic Scotland Ltd Cell contamination assay
WO2020072545A1 (en) * 2018-10-01 2020-04-09 Lundquist Institute For Biomedical Innovation At Harbor-Ucla Medical Center MicroRNA TRANSCRIPTIONAL SIGNATURE (miR-TS) EVALUATION OF STEM CELLS

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007073737A1 (en) * 2005-12-29 2007-07-05 Exiqon A/S Detection of tissue origin of cancer
WO2008112283A2 (en) * 2007-03-12 2008-09-18 Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Microrna profiling of androgen responsiveness for predicting the appropriate prostate cancer treatment
WO2009036332A1 (en) * 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050123952A1 (en) * 2003-09-04 2005-06-09 Griffey Richard H. Methods of rapid detection and identification of bioagents using microRNA
JP5697297B2 (ja) * 2004-05-14 2015-04-08 ロゼッタ ジノミクス リミテッド マイクロｎａｓおよびその使用
CN101316935B (zh) * 2006-11-28 2012-02-29 博奥生物有限公司 一种用于诊断食道癌的芯片
CN101622350A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为干预治疗靶标的miR-126调控基因和通路
WO2009062503A1 (en) * 2007-11-14 2009-05-22 Dandrit Biotech A/S Micro rnas as markers of the functional state of a dendritic cell
CA2717030A1 (en) * 2008-02-28 2009-09-03 The Ohio State University Research Foundation Microrna signatures associated with cytogenetics and prognosis in acute myeloid leukemia (aml) and uses thereof
US20100003674A1 (en) * 2008-07-03 2010-01-07 Cope Frederick O Adult stem cells, molecular signatures, and applications in the evaluation, diagnosis, and therapy of mammalian conditions
WO2010082039A1 (en) * 2009-01-19 2010-07-22 Sistemic Scotland Limited Methods employing non-coding rna expression assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007073737A1 (en) * 2005-12-29 2007-07-05 Exiqon A/S Detection of tissue origin of cancer
WO2008112283A2 (en) * 2007-03-12 2008-09-18 Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Microrna profiling of androgen responsiveness for predicting the appropriate prostate cancer treatment
WO2009036332A1 (en) * 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113658636A (zh) * 2021-07-22 2021-11-16 未来智人再生医学研究院(广州)有限公司 一种评估多能干细胞质量的方法
CN113658636B (zh) * 2021-07-22 2025-08-05 未来智人再生医学研究院(广州)有限公司 一种评估多能干细胞质量的方法

Also Published As

Publication number Publication date
SG190837A1 (en) 2013-07-31
BR112013004202A2 (pt) 2019-09-24
IL224888B (en) 2020-10-29
SG10201506595QA (en) 2015-10-29
CA2847151C (en) 2023-11-28
US20200080148A1 (en) 2020-03-12
EP2609217A1 (en) 2013-07-03
KR20140006775A (ko) 2014-01-16
JP2013535979A (ja) 2013-09-19
EP2609217B1 (en) 2019-03-27
US20130196875A1 (en) 2013-08-01
CA2847151A1 (en) 2012-03-01
GB201014049D0 (en) 2010-10-06
WO2012025709A1 (en) 2012-03-01
US20230183803A1 (en) 2023-06-15
US20170218446A1 (en) 2017-08-03
AU2011294936A1 (en) 2013-04-11

Similar Documents

Publication Publication Date Title
US20230183803A1 (en) Cell characterisation
Liu et al. Purification and characterization of human neural stem and progenitor cells
Sasagawa et al. Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads
Messmer et al. Transcriptional heterogeneity in naive and primed human pluripotent stem cells at single-cell resolution
Pollen et al. Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex
Wilson et al. Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499
US10626445B2 (en) Early developmental genomic assay for characterizing pluripotent stem cell utility and safety
Ortmann et al. Naive pluripotent stem cells exhibit phenotypic variability that is driven by genetic variation
CN114875118B (zh) 确定细胞谱系的方法、试剂盒和装置
US20220356461A1 (en) High-throughput single-cell libraries and methods of making and of using
Petropoulos et al. Single‐cell RNA sequencing: revealing human pre‐implantation development, pluripotency and germline development
Stelzer et al. Global analysis of parental imprinting in human parthenogenetic induced pluripotent stem cells
Martinez Analysis of gene expression and gene silencing in human macrophages
Juzenas et al. inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples
MacArthur et al. Generation and comprehensive characterization of induced pluripotent stem cells for translational research
Ruan et al. Expression profile of long non-coding RNAs during the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocyte-like cells
Sampath et al. Identifying translationally regulated genes during stem cell differentiation
Pal et al. A panel of tests to standardize the characterization of human embryonic stem cells
Teske et al. Targeted CRISPR-Cas9 screening identifies transcription factor network controlling murine haemato-endothelial fate commitment
Brodská Single-cell RNA sequencing in leukemia
Tsai et al. Microarray and gene-clustering analysis
Schmauch et al. Global MicroRNA Profiling of Vascular Endothelial Cells
Wang et al. Single-cell microRNA/mRNA co-sequencing reveals non-genetic heterogeneity and novel regulatory mechanisms
Zambon et al. Microarray analysis of embryonic stem cells and differentiated embryoid bodies
Ciani Regulatory modules discovery and mesenchymal stem cells characterization from high-throughput cancer genomics data

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20131120