CA2847151C - Assessing stem cells for contamination using microrna expression profiles - Google Patents

Assessing stem cells for contamination using microrna expression profiles Download PDF

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Publication number
CA2847151C
CA2847151C CA2847151A CA2847151A CA2847151C CA 2847151 C CA2847151 C CA 2847151C CA 2847151 A CA2847151 A CA 2847151A CA 2847151 A CA2847151 A CA 2847151A CA 2847151 C CA2847151 C CA 2847151C
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cells
micro
cell
stem cells
rna
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French (fr)
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CA2847151A1 (en
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Vincent O'brien
Chris Hillier
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Sistemic Scotland Ltd
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Sistemic Scotland Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/112Disease subtyping, staging or classification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2847151A 2010-08-23 2011-08-19 Assessing stem cells for contamination using microrna expression profiles Active CA2847151C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1014049.9 2010-08-23
GBGB1014049.9A GB201014049D0 (en) 2010-08-23 2010-08-23 Cell characterisation
PCT/GB2011/001241 WO2012025709A1 (en) 2010-08-23 2011-08-19 Cell characterisation

Publications (2)

Publication Number Publication Date
CA2847151A1 CA2847151A1 (en) 2012-03-01
CA2847151C true CA2847151C (en) 2023-11-28

Family

ID=42984495

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2847151A Active CA2847151C (en) 2010-08-23 2011-08-19 Assessing stem cells for contamination using microrna expression profiles

Country Status (12)

Country Link
US (4) US20130196875A1 (enExample)
EP (1) EP2609217B1 (enExample)
JP (1) JP2013535979A (enExample)
KR (1) KR20140006775A (enExample)
CN (1) CN103403180A (enExample)
AU (1) AU2011294936A1 (enExample)
BR (1) BR112013004202A2 (enExample)
CA (1) CA2847151C (enExample)
GB (1) GB201014049D0 (enExample)
IL (1) IL224888B (enExample)
SG (2) SG10201506595QA (enExample)
WO (1) WO2012025709A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2859119A2 (en) * 2012-06-12 2015-04-15 Institute for Systems Biology Complex rna composition of bodily fluids
WO2017160880A1 (en) * 2016-03-14 2017-09-21 Aelan Cell Technologies, Inc. Compositions and methods for the quality control of stem cell preparations
SG11201809902QA (en) 2016-05-09 2018-12-28 Sistemic Scotland Ltd Functional prediction of cellular functions by means of microrna expression profiling in mesenchymal stem cells
JP6629770B2 (ja) * 2017-01-19 2020-01-15 シスメックス株式会社 細胞の分化状態を評価する方法
JP6918062B2 (ja) * 2017-01-19 2021-08-11 シスメックス株式会社 細胞の分化状態を評価する方法
GB2570404A (en) * 2018-01-22 2019-07-24 Sistemic Scotland Ltd Cell contamination assay
WO2020072545A1 (en) * 2018-10-01 2020-04-09 Lundquist Institute For Biomedical Innovation At Harbor-Ucla Medical Center MicroRNA TRANSCRIPTIONAL SIGNATURE (miR-TS) EVALUATION OF STEM CELLS
CN113658636B (zh) * 2021-07-22 2025-08-05 未来智人再生医学研究院(广州)有限公司 一种评估多能干细胞质量的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050123952A1 (en) * 2003-09-04 2005-06-09 Griffey Richard H. Methods of rapid detection and identification of bioagents using microRNA
JP5697297B2 (ja) * 2004-05-14 2015-04-08 ロゼッタ ジノミクス リミテッド マイクロnasおよびその使用
EP1966390A1 (en) * 2005-12-29 2008-09-10 Exiqon A/S Detection of tissue origin of cancer
CN101316935B (zh) * 2006-11-28 2012-02-29 博奥生物有限公司 一种用于诊断食道癌的芯片
CN101622350A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为干预治疗靶标的miR-126调控基因和通路
WO2008112283A2 (en) * 2007-03-12 2008-09-18 Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Microrna profiling of androgen responsiveness for predicting the appropriate prostate cancer treatment
WO2009036332A1 (en) * 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
WO2009062503A1 (en) * 2007-11-14 2009-05-22 Dandrit Biotech A/S Micro rnas as markers of the functional state of a dendritic cell
CA2717030A1 (en) * 2008-02-28 2009-09-03 The Ohio State University Research Foundation Microrna signatures associated with cytogenetics and prognosis in acute myeloid leukemia (aml) and uses thereof
US20100003674A1 (en) * 2008-07-03 2010-01-07 Cope Frederick O Adult stem cells, molecular signatures, and applications in the evaluation, diagnosis, and therapy of mammalian conditions
WO2010082039A1 (en) * 2009-01-19 2010-07-22 Sistemic Scotland Limited Methods employing non-coding rna expression assays

Also Published As

Publication number Publication date
SG190837A1 (en) 2013-07-31
BR112013004202A2 (pt) 2019-09-24
IL224888B (en) 2020-10-29
SG10201506595QA (en) 2015-10-29
US20200080148A1 (en) 2020-03-12
EP2609217A1 (en) 2013-07-03
KR20140006775A (ko) 2014-01-16
JP2013535979A (ja) 2013-09-19
EP2609217B1 (en) 2019-03-27
US20130196875A1 (en) 2013-08-01
CA2847151A1 (en) 2012-03-01
GB201014049D0 (en) 2010-10-06
WO2012025709A1 (en) 2012-03-01
CN103403180A (zh) 2013-11-20
US20230183803A1 (en) 2023-06-15
US20170218446A1 (en) 2017-08-03
AU2011294936A1 (en) 2013-04-11

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