CN1034023C - Processing method of citric acid or sodium citrate - Google Patents
Processing method of citric acid or sodium citrate Download PDFInfo
- Publication number
- CN1034023C CN1034023C CN95111000A CN95111000A CN1034023C CN 1034023 C CN1034023 C CN 1034023C CN 95111000 A CN95111000 A CN 95111000A CN 95111000 A CN95111000 A CN 95111000A CN 1034023 C CN1034023 C CN 1034023C
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- China
- Prior art keywords
- citric acid
- semen maydis
- maydis powder
- fermentor tank
- citrate
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Abstract
The present invention relates to a preparation method of citric acid or sodium citrate. Maize meal or dry potato flour is used as raw material, and then, water and high-temperature amylase are added to the raw material to be liquefied at high temperature and filtered; subsequently, protein and nutritive substances are added to the mixture to be inoculated into a seed tank to obtain citric acid by a calcium salt method extracting step, etc., or sodium carbonate is added to calcium citrate when a calcium salt method is used for preparing the calcium citrate to prepare sodium citrate by a double decomposition method. Thus, the purpose of using maize meal as raw material to be directly fermented to prepare citric acid can be realized; meanwhile, the production cost is reduced, the acid yield is improved, and the fermentation period is shortened.
Description
The present invention relates to a kind of method for preparing citric acid or Trisodium Citrate with the fermentation means.
It is raw material that the preparation citric acid adopts potato class powder usually, as Chinese patent GK85108564 disclosed " with black-koji mould fermentation coproduction diosgenin and citric acid "; Have also that to adopt wheat-flour be raw material, as Chinese patent CN1072456A disclosed " utilizing wheat-flour production methods of citric acid "; At present domesticly adopt W-Gum to add a small amount of fermentation of corn starch in addition to extract citric acid, it extracts citric acid after heating liquefaction, sterilization cooling, connecing seeding tank, its fermentation period 96 hours, acid production rate 10%, the price of W-Gum is per ton approximately three over thousands of yuan at present.
The purpose of this invention is to provide a kind of preparation method, raw material all adopts Semen Maydis powder, citric acid or preparation Trisodium Citrate are extracted in direct fermentation, change " because Semen Maydis powder protein content height, nutrition are too abundant; existing citric acid production bacterial strain can't utilize the substratum of this high nutrition to carry out metabolism accumulation citric acid, can't be that fermenting raw materials is directly extracted citric acid with Semen Maydis powder." this traditional concept, simultaneously, reduce production costs, improve acid production rate, shorten fermentation period; Can also prepare Trisodium Citrate.
For achieving the above object, the present invention adopts following technical scheme: through the arrangement of the visible Semen Maydis powder of electron microscope scanning closely, be dark cave-shaped structure, liquefaction process routinely can't reach the liquefaction effect, thereby with Semen Maydis powder and water by weight 1: 4-1: 5 size mixing the back at the liquefied pot internal heating, the alpha-amylase that adds 6-12 unit during temperature to 65 ℃ by every gram Semen Maydis powder, be maintained until liquefaction fully when the alpha-amylase that adds 4-8 unit during temperature to 90 ℃ again by every gram Semen Maydis powder, temperature to 95 ℃; Adopt quick filtration will contain superfluous protein and the Semen Maydis grit of the solid substance that can not ferment separates with liquefier, liquefier is dropped into fermentor tank; The liquefier overwhelming majority after filtering is the short chain dextrin of 7-8DP, substantially do not contain protein and other nutrition, therefore must add partly protein and nutritive substance, citric acid growth bacterium is growth and breeding and metabolism accumulation citric acid normally, so the Semen Maydis grit of step gained is to fermentor tank in the adding, add-on is the 5-25% of Semen Maydis grit gross weight, certainly, also can add other that satisfy above-mentioned needs and be equal to nutritive substance; The sterilization of heating stops to heat and beginning fast cooling when heating to 85 ℃, when reducing to 37 ℃ seeding tank is inserted fermentor tank; The liquid that fermentation back gained is contained citric acid extracts citric acid with calcium salt method, or adds yellow soda ash when producing citrate of lime in the calcium salt method process, utilizes double decomposition to extract Trisodium Citrate.The used Semen Maydis powder raw material of aforesaid method also can adopt Rhizoma Dioscoreae powder.
By above scheme as seen, the size mixing liquefaction of heating makes protein coagulating during to 95 ℃ to Semen Maydis powder because the present invention adopts, filtering protein and nutritive substance, adjust the nutrition of substratum by adding an amount of Semen Maydis grit again, make and utilize Semen Maydis powder can be achieved for raw material direct fermentation citric acid or Trisodium Citrate; Simultaneously, contrast visible unusual effect of the present invention by the following table data:
| The raw material composition | Fermentation period | Acid production rate | Cost of material |
| Semen Maydis powder | 54 hours | 12％ | 1620 yuan/ton |
| W-Gum adds a small amount of Semen Maydis powder | 96 hours | 10％ | 3280 yuan/ton |
In addition, this preparation method also has following advantage: owing to the dissolved oxygen effect that improves greatly in the fermenting process, accelerated to produce the speed of acid, shortened fermentation period, through the measuring and calculating of citric acid production whole process, finished product economize on electricity 400 per ton is spent; The citric acid production total recovery improves 10%, having manufactured a finished product 80% reaches USP standard.Below also do not comprise the development and use of overall economic efficiency, scale and benefit and byproduct.
Six embodiment below are provided, and the invention will be further described.
Embodiment one: (1) liquefaction will be sized mixing according to weight ratio without doing the Semen Maydis powder and the water that take off embryo by the liquefied pot capacity at 1: 5, adopt steam to heat then, add alpha-amylase during temperature to 65 ℃, add-on depends on the weight that drops into Semen Maydis powder, every gram Semen Maydis powder adds 9 units, when continuing to heat to 90 ℃ again the amount by every gram Semen Maydis powder 6 units add alpha-amylase, keep this temperature value until liquefaction fully during temperature to 95 ℃.
(2) filter the step product of going up that will liquefy fully and isolate Semen Maydis grit and liquefier, whole liquefiers are squeezed into fermentor tank with pump by pipeline with the sheet frame fast press-filtration.
(3) adjust the 8% adding fermentor tank that substratum is isolated press filtration the Semen Maydis grit gross weight, opening three road steam then carries out disinfection, open water coolant during temperature to 85 ℃ and carry out fast cooling, during temperature to 37 ℃ seeding tank is inserted fermentor tank, the citric acid production bacterial strain wherein acid flat board of Semen Maydis powder is tamed rejuvenation repeatedly.
(4) liquid that contains citric acid solution after extract adopting at present general calcium salt method to fermentation extract the citric acid crystal.
Embodiment two:
(1) liquefaction will be sized mixing according to weight ratio without doing the Semen Maydis powder and the water that take off embryo by the liquefied pot capacity at 1: 5, adopt steam to heat then, add alpha-amylase during temperature to 65 ℃, every gram Semen Maydis powder adds 6 units, when continuing to heat to 90 ℃ again the amount by every gram Semen Maydis powder 4 units add alpha-amylase, keep this temperature value until liquefaction fully during temperature to 95 ℃.
(2) filter the step product of going up that will liquefy fully and isolate Semen Maydis grit and liquefier, whole liquefiers are squeezed into fermentor tank with pump by pipeline with whizzer.
(3) adjust the 5% direct fermentor tank that adds that substratum is isolated (1) step filtered the liquefier gross weight of Semen Maydis grit, opening three road steam then carries out disinfection, carry out fast cooling during temperature to 85 ℃, during temperature to 37 ℃ seeding tank is inserted fermentor tank, the citric acid production bacterial strain wherein acid flat board of Semen Maydis powder is tamed rejuvenation repeatedly.
(4) liquid that contains citric acid solution after extract adopting at present general calcium salt method to fermentation extract the citric acid crystal.
Embodiment three:
(1) liquefaction will be sized mixing according to weight ratio without doing the Semen Maydis powder and the water that take off embryo by the liquefied pot capacity at 1: 5, adopt steam to heat then, add alpha-amylase during temperature to 65 ℃, every gram Semen Maydis powder adds 2 units, when continuing to heat to 90 ℃ again the amount by every gram Semen Maydis powder 8 units add alpha-amylase, keep this temperature value until liquefaction fully during temperature to 95 ℃.
(2) filter the step product of going up that will liquefy fully and isolate Semen Maydis grit and liquefier, whole liquefiers are squeezed into fermentor tank with pump by pipeline with vacuum filtration.
(3) adjust the 20% direct fermentor tank that adds that substratum is isolated () step filtered the liquefier gross weight of Semen Maydis grit, opening three road steam then carries out disinfection, carry out fast cooling during temperature to 85 ℃, during temperature to 37 ℃ seeding tank is inserted fermentor tank, the citric acid production bacterial strain wherein acid flat board of Semen Maydis powder is tamed rejuvenation repeatedly.
(4) liquid that contains citric acid solution after extract adopting at present general calcium salt method to fermentation extract the citric acid crystal.
Embodiment four:
(1) liquefaction is sized mixing Rhizoma Dioscoreae powder and water according to weight ratio by the liquefied pot capacity at 1: 4, adopt steam to heat then, add alpha-amylase during temperature to 65 ℃, every gram Rhizoma Dioscoreae powder adds 6 units, when continuing to heat to 90 ℃ again the amount by every gram Semen Maydis powder 4 units add alpha-amylase, keep this temperature value until liquefaction fully during temperature to 95 ℃.
(2) filter the step product of going up that will liquefy fully and isolate sweet potato slag and liquefier, whole liquefiers are squeezed into fermentor tank with pump by pipeline with filter press.
(3) adjusting substratum will account for (1) step filtered and isolate 15% Semen Maydis grit of sweet potato slag liquefier gross weight and directly add fermentor tank, opening three road steam then carries out disinfection, carry out fast cooling during temperature to 85 ℃, during temperature to 37 ℃ seeding tank is inserted fermentor tank, citric acid production bacterial strain wherein adopts general Rhizoma Dioscoreae powder bacterial strain.
(4) liquid that contains citric acid solution after extract adopting at present general calcium salt method to fermentation extract the citric acid crystal.
Embodiment five:
(1) Semen Maydis powder and water were sized mixing by weight 1: 4 by the liquefied pot capacity through the Semen Maydis powder of doing after taking off embryo, adopt steam heating then, add alpha-amylase during temperature to 65 ℃, add-on is by 7 units of every gram Semen Maydis powder, add by 6 units of every gram Semen Maydis powder again when continuing to be heated to 90 ℃, keep this temperature when temperature rises to 95 ℃ to liquefaction fully.
(2) filter 75% of the step product weight that goes up that will liquefy fully and carry out filter press, isolate Semen Maydis grit and liquefier.
(3) adjust substratum and all squeeze into fermentor tank through 25% liquefier of the liquefier of Plate Filtration and filtered 75%, opening three road steam then carries out disinfection, carry out fast cooling during temperature to 85 ℃, during temperature to 37 ℃ seeding tank is inserted fermentor tank, the citric acid production bacterial strain wherein acid flat board of Semen Maydis powder is tamed rejuvenation repeatedly.
(4) liquid that contains citric acid solution after extract adopting at present general calcium salt method to fermentation extract the citric acid crystal.
Embodiment six:
Present embodiment provides the method for preparing Trisodium Citrate according to the foregoing description, when producing citrate of lime in the calcium salt method process, promptly stop citric acid further extraction and according to following replacement(metathesis)reaction equation:
Claims (2)
1, the preparation method of a kind of citric acid or Trisodium Citrate, this method comprises:
(1) with Semen Maydis powder and water by weight 1: 4-1: 5 post-heating of sizing mixing, the alpha-amylase that adds 6-12 unit during temperature to 65 ℃ by every gram Semen Maydis powder, be maintained until liquefaction fully when the alpha-amylase that adds 4-8 unit during temperature to 90 ℃ again by every gram Semen Maydis powder, temperature to 95 ℃;
(2) adopt quick filtration will contain superfluous protein and the Semen Maydis grit of the solid substance that can not ferment separates with liquefier, liquefier is dropped into fermentor tank;
(3) Semen Maydis grit of getting the 5-25% of step gained Semen Maydis grit gross weight adds fermentor tank;
(4) sterilization of heating stops heating and beginning fast cooling when heating to 85 ℃, when reducing to 37 ℃ seed liquor is inserted fermentor tank;
(5) liquid that the back gained contains citric acid that will ferment extracts citric acid with calcium salt method, or from further preparing Trisodium Citrate with the citrate of lime that produces the calcium salt method leaching process.
2, the preparation method of citric acid according to claim 1 or Trisodium Citrate, its said Semen Maydis grit of (3) step can substitute with the liquid liquid of (1) step gained filtered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95111000A CN1034023C (en) | 1995-04-08 | 1995-04-08 | Processing method of citric acid or sodium citrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95111000A CN1034023C (en) | 1995-04-08 | 1995-04-08 | Processing method of citric acid or sodium citrate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1112606A CN1112606A (en) | 1995-11-29 |
| CN1034023C true CN1034023C (en) | 1997-02-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95111000A Expired - Lifetime CN1034023C (en) | 1995-04-08 | 1995-04-08 | Processing method of citric acid or sodium citrate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1034023C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077138C (en) * | 1999-11-19 | 2002-01-02 | 天津市工业微生物研究所 | Fermentation technology using rice as raw material to produce citric acid |
| CN101555497B (en) * | 2009-05-20 | 2012-05-30 | 中粮生物化学（安徽）股份有限公司 | Preparation method of citric acid fermentation solution |
| CN102952830A (en) * | 2012-08-29 | 2013-03-06 | 太仓市茂通化建有限公司 | Method for preparing citric acid through fermenting cheap dried sweet potatoes by utilizing aspergillus niger |
| CN106071112A (en) * | 2016-06-13 | 2016-11-09 | 北京昕大洋科技发展有限公司 | A kind of production method of saccharomyces neoformans source feedstuff |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3904684A (en) * | 1972-05-03 | 1975-09-09 | Takeda Chemical Industries Ltd | Method for producing sodium citrate dihydrate |
| US4767707A (en) * | 1985-07-27 | 1988-08-30 | Basf Aktiengesellschaft | Immobilization of enzymes with a water-soluble glycidyl ether |
| CN1043315A (en) * | 1989-11-17 | 1990-06-27 | 李芳德 | The improvement of method for extracting citric acid |
| CN1072456A (en) * | 1992-08-28 | 1993-05-26 | 宁夏盐池县柠檬酸厂 | Utilize wheat-flour production methods of citric acid |
-
1995
- 1995-04-08 CN CN95111000A patent/CN1034023C/en not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3904684A (en) * | 1972-05-03 | 1975-09-09 | Takeda Chemical Industries Ltd | Method for producing sodium citrate dihydrate |
| US4767707A (en) * | 1985-07-27 | 1988-08-30 | Basf Aktiengesellschaft | Immobilization of enzymes with a water-soluble glycidyl ether |
| CN1043315A (en) * | 1989-11-17 | 1990-06-27 | 李芳德 | The improvement of method for extracting citric acid |
| CN1072456A (en) * | 1992-08-28 | 1993-05-26 | 宁夏盐池县柠檬酸厂 | Utilize wheat-flour production methods of citric acid |
Non-Patent Citations (1)
| Title |
|---|
| 金其荣等《有机酸发酵工艺学》 1989.1.1 轻工业出版社 * |
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| Publication number | Publication date |
|---|---|
| CN1112606A (en) | 1995-11-29 |
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Expiration termination date: 20150408 Granted publication date: 19970212 |