CN1034020A - Preparation and the application of the plant of biological value have been improved - Google Patents

Preparation and the application of the plant of biological value have been improved Download PDF

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CN1034020A
CN1034020A CN88108058A CN88108058A CN1034020A CN 1034020 A CN1034020 A CN 1034020A CN 88108058 A CN88108058 A CN 88108058A CN 88108058 A CN88108058 A CN 88108058A CN 1034020 A CN1034020 A CN 1034020A
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plant
micro
water
substratum
trace element
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勃达尼奥加·尼·弗加科斯
勃达尼·拉茨罗
法宾·卡太林·尼皮尔
法宾·米克罗斯
伦亚·皮特
埃拉哈姆·苏茨萨
凯夫·迪伯
凯夫·安得烈·尼森托亚
卡波维茨·伊斯特凡
勃格耐尔·琼斯
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Aaron Skin
Karbowitz Ess Van
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Aaron Skin
Karbowitz Ess Van
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Priority claimed from HU523587A external-priority patent/HU202913B/en
Priority claimed from HU885141A external-priority patent/HU202914B/en
Priority claimed from HU885142A external-priority patent/HUT53503A/en
Priority claimed from HU514088A external-priority patent/HU204186B/en
Priority claimed from HU885144A external-priority patent/HUT53504A/en
Priority claimed from HU885145A external-priority patent/HU202915B/en
Priority claimed from HU514388A external-priority patent/HU204187B/en
Application filed by Aaron Skin, Karbowitz Ess Van filed Critical Aaron Skin
Publication of CN1034020A publication Critical patent/CN1034020A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/02—Preparations for cleaning the hair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

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Abstract

The present invention relates to prepare the plant of having improved biological value or the method for its part, other draws together being added with concentration is 10 -9～10 -1Culturing plants seed, tissue or cell in one or more micro-substratum of M.
The resulting plant that high-micro-element content arranged or its part or its extract are used to prepare food, medicine or make-up composition.

Description

Preparation and the application of the plant of biological value have been improved
The present invention relates to be used to prepare the plant of having improved biological value, at first be the cultural method of plant embryo and plant organ, tissue and cell, and the activeconstituents that so makes is as the application of makeup, food and medical composition.
All organisms all are elementary composition by periodic system.11 kinds of elements that can see intravital this system of living organism in a large number are: carbon, hydrogen, oxygen, nitrogen, sulphur, calcium, phosphorus, potassium, sodium, chlorine, magnesium.In addition, with other 16 kinds of so-called trace elements that are present in live organism in a small amount iron, zinc, copper, manganese, nickel, cobalt, molybdenum, selenium, chromium, iodine, fluorine, tin, silicon, vanadium, arsenic, lithium are arranged.
Except that these elements, the existence of boron also is indispensable for the growth of higher plant.
Above-mentioned element mostly is the element between No. 23 to No. 34 greatly.Also have gallium and germanium in this zone of periodic system, its biological action is not still understood at present, is remained further to be understood fully.
By the above-mentioned fact as can be seen, element at first is that the existence of appropriate amount trace element is crucial for life.
Although used different compost and fertilizer,, and cause the trace element in the present soil fewer and feweri because agricultural production growth is very rapid.
The result has just caused the problem of trace element supply in soil-plant-humans and animals food chain, and causes trace element more and more to lack.
Wherein sixty-four dollar question is that humans and animals is rely therefrom and to be obtained first source of trace element, i.e. micro-under-supply in the plant.
Influence that four principal elements of micronutrient levels are in the plant:
A) genus of plant, kind;
B) type of the required soil of plant-growth;
C) weather during the plant-growth and season condition;
D) etap of plant.
According in the specification sheets of the present invention, optimizing ground practice and utilized these possibilities, promptly certain plants itself can or be organized middle and high concentration enrichment some trace element with biologically active form at it by progressively adapting to.
For example, the racemosus Radix Astragali (Astragalus racemosus) and the false Touch-me-notAction Plant of armful stem (Neptunia amplexicaulis) can gather selenium.Forest tupelo gum (Nyssa sylvatica) is Duoed 100 times by the amount of gathering cobalt in the same soil than other plant.
Can be observed similar difference between the leguminous plants, grass and the cereal grass that grow on the same soil, wherein Ca, the Ni, Fe, Cu and the Zn that gather of leguminous plants is more.Gramineae and cereal can be gathered Mn, Mo, particularly silicon better.
During the development of plants, these elements can reduce in the whole plants body or increase, and perhaps can become stable.
On the other hand, micronutrient levels reduces and can cause different symptoms in the humans and animals body.
Relevant these symptoms (see Trace elements in Human and Animal Nutrition for details, Eric.J.Underwood; Academic Press, New York, San Francisco, London, 1977) and use these trace element biological actions that bring to be summarized as follows according to method of the present invention:
Iron (Fe 2+) having king-sized importance in the intravital existence of people, its total content is 4 to 5g, is mainly seen in oxyphorase and the myohaemoglobin, flavoprotein contains the iron of less than 1%.Iron deficiency can cause the serious disease of blood formation aspect, and this is the most obvious sign of iron deficiency, and sideropenia also can show symptoms such as had palpitation, glossalgia, gastritis, dysphagia.
Copper also is very important in blood forms, and its total content reaches 80mg.Known have some protein to contain copper, as erythrocuprein, cerebrocuprein, hepatocuprein, cytocuprein.These enzymes (being hemocuprein) have vital role in the inhibition of oxidation mechanism and radical with in forming.It can influence hair growth, because only just can form disulfide linkage at Keratin sulfate in the presence of it between synthesis phase, and because it is to make tyrosine form the part of the polyphenoloxidase of melanocyte (it has makes hair become the effect of ash).
Proved that nickel mainly has vital role under the situation of myocardial infarction and burn.Nickel lacks the effect that also reduces the liver hydrogenase.
Seeing biological intravital manganese total amount is 12-20mg, mainly is to be present in the plastosome.Slow down osteogenesis because of synthetic minimizing of chondroitin sulfate and amidohexose during manganese deficiency, cause ataxia, refresh function is impaired, lipid metabolism changes and cholesterol is synthetic reduces.The active part of several fermentoids such as lytic enzyme, quinases, decarboxylase, transferring enzyme etc. reduces.
Molybdenum can see in the various tissues by lower concentration.Flavoprotein and XOD are the metalloenzyme that contains molybdenum, and its shortage can cause carious tooth.
The content of selenium in liver and kidney is maximum.Had recognized that its importance in oxidation mechanism recently.It is the composition of Selenoperoxidase, and the latter can protect body to avoid the influence of deleterious superoxide:
Therefore selenium can make cell directly not suffer oxygenizement, can be by adding selenium or vitamin-E prevents exudative diathesis.Selenium is similar to vitamin-E as its effect of antioxidant, and they both all can protect microbial film to avoid oxidation to attack and can prevent exudative diathesis.The cytopigment that contain selenium may work in the oxidation-reduction mechanism of tricarboxylic acid cycle.Selenium deficiency can cause growth disorder, sexual dysfunction, pancreas fibrosis, hepatic insufficiency, exudative diathesis.
The detected result of being done showed in the past, selenium tool in prophylaxis of tumours play a very important role (Mark F, Mc.Carty, Medical Hypothesis 14,213-225,1984).
According to the test-results of adding 600mcg selenium every day, it can make the people exempt from several infringements that cause the chemical inducer of tumour, and helps DNA to repair.By check as can be seen, obviously reduced although the fact is its toxicity, the effectiveness that organically combines selenium at first is the same.This check be to use Sodium Selenite and with enrichment the yeast fungus of selenium (Schwarz K., 60-doy toxicity trial with selenium yeast II .1975 December 21 did not deliver manuscript) of carrying out.
Contain a considerable amount of silicon in the skin, this element is the integral part as the mucopolysaccharide of skin basic structure component.According to Schwarz(Proc.Natl.Acad.Sci, USA70,1608,1973) detected result, be no less than 330-554ppm in conjunction with silicone content in the hyaluronic acid.The α chain that also proves collagen protein simultaneously contains 3-6 Siliciumatom at least.Silicon can directly influence the content of phosphorus, is indispensable to bone forming and growth.
The iodine of 70-80% sees in the Tiroidina, and iodine deficiency can make cellular oxidation impaired, and plastosome internal respiration effect increases.It can influence the differentiation and the growth of cell, and comprising by the controlling gene expression influences RNA and protein synthesis.
Intravital fluorine concentrates in bone and the tooth, and it can suppress carious tooth and osteoporosis.
The intravital chromium of the machine that sees has only seldom amount, but its effect is very important.The typical chromium symptom that lacks is: hypoevolutism, sugar, fat and protein metabolism disorder.
Few known to the effect of relevant tin, but it is definitely indispensable for growth.
Vanadium content in vivo is very low.It is influential to reproduction and lipid metabolism.It stops cholesterol to be synthesized, reduces blood plasma phosphatide and cholesterol levels, and reduces aortal cholesterol concentration.It can stop carious tooth to form.
A spot of arsenic (30ppb) can produce favourable influence to growth, can be used as the selenium antagonism agent when its concentration is higher, and has a negative impact.
Though lithium just is used to treat psychosis (manic depression) for a long time, it is not still understood in the intravital physiological action of machine.Have recognized that the inhibition activity of lithium recently to simplexvirus.
The physiological action that boron is brought into play Mammals is still clear, but in view of its effect in the synthetic flavonoid of higher plant, so be very important.
Recognize just that up in recent years germanium is indispensable in mammalian body.The result shows according to current research, and germanium plays an important role in oxide compound mechanism, and it acts on similar in appearance to selenium.
It has very strong antitumor action, particularly the function of tumor of commute transfer is better, and Mice Bearing Lewis Lung Cancer had a significant impact (Kumano N., Ishikawa T., Koinumaru S., Deng, Antitumour effect of organogermanium compound G-132 on the Lewis-Lung carcinoma/3 LL/in C57 PL/6/86/mice Tohoku, J.Exp.Med.1985 146/1,94-104).
What we should specify here is that it is effective representational organic germanium compounds having been carried out check and found.Germanium may be by modifying effect (the Sato I. that its expection is brought into play in immune response, Yan B.D., Nishimura I., Tanaka N-, Inhibition of tumor growth and metastasis in association with modification of immune response by novel organic germanium compound, J.Biol.Response Modif., 1,985 4/2,159-168).
Manganese has multiple effect in body.It participates in the katalysis of several enzymes.The most important thing is wherein to guarantee that those participate in the activity of the enzyme of nucleic acid, protein synthesis.
Calcium is present in the body with considerable quantity, and it has vital role in bone and tooth form.
The needed aluminium of body is less than μ g every day 1.It is the auxiliary agent of succinyl-dehydrogenase.
The importance of cobalt in blood forms is known, and cobalt and iron betransported together.It is to promote methylmalonyl Co A to be converted into the vitamins B of succinyl-Co A 12Component.5-methyl tetrahydrochysene leaf acyl-homocysteine methyltransgerase also is a kind of important enzyme that the catalysis homocysteine generates methionine(Met).Its daily requirement is 3mg/kg.
1/5th zinc is found in the skin.Zinc has very big importance in liver, it is present in the metal thionine in liver.The more tissue of its content has eye (139-436ppm), testis (120ppm), prostate gland (859ppm), nail (93-292ppm) and hair (92-255ppm).It is and α in blood 2Macroglobulin and albumin bound exist.
Stress reaction can reduce zinc level.Reduce because of thymidine kinase activity when lacking zinc and DNA is synthetic and cell division capacity reduces and causes growthing lag, symptoms such as alopecia, dermatitis, skin deformation and chronic skin ulcer formation occur.Make the hyaluronic acid contents in the skin reduce (Thomson, R.W., J.Nutr.105,154,1975), synthetic reduce (Mc Clain BBA304,457,1973) of collagen in addition.
Therefore zinc plays an important role in collagen, DNA, RNA and protein synthesis; In other words, the existence of zinc is indispensable for gene activation.Zinciferous in addition enzyme has pancreas carboxypeptidase, serum lactic dehydrogenase (LDH), propanedioic acid desaturase (MDH), ethanol dehydrogenase (ADH) and alkaline phosphatase.Human body essential zinc every day is 15-25mg.
Should be mentioned that at last precious metals such as gold and silver, platinum it be unclear that the effect of human body, but can be with them as medicine.As the existence form of silver, argyrol is mainly used in sterilization; Gold is used for the treatment of rheumatic joint disease; Platinum is used for the treatment of tumour.
By the above-mentioned fact as can be seen, in vivo, the trace element of particularly keeping proper level in the human body is crucial.
By replenishing suitable trace element, several unidentified illness of human body have repeatedly been cured.
According to the present invention, gathering trace element in suitable plant materials, and with valuable form biologically as being directly applied for mammiferous makeup, foodstuffs compositions or medical composition.According to the kind of trace element, it can be used for treating or curing the above-mentioned biology disorder relevant with lacking these trace elements.The known trace element that gives inorganic salt form is invalid to human body, because human body can not suitably utilize the trace element (as ionic composition) of this form.A kind of more advantageous forms is when required metal ion is attached on the carrier molecule after it takes in human body and enters the active centre of known enzyme, and can せ exempting from the mediocre δ glutinous rehmannia of multiple the servants  widely different numerous ⒒ of strange  joke  plutonium, to bathe the 7 thick  ⒘ towering ń fish hawk of the quilt Guang cherry ┰ tadpole oxime  excuse  famine small drum used in the army in ancient China  that shines that utters a sound or a word disrespectful
The method according to this invention can use different plants, medicinal plant, agricultural plants or wild plant or its part to prepare trace element-carrier complexes, and the former is for meeting the organic double compound of above-mentioned requirements fully.
According to the present invention, the seed of different plants, tissue or cell are to grow in that to have added concentration be 10 -9～10 -1In the substratum of the trace element of M.
Through after processing or extracting, contain foodstuffs compositions, medical composition or the makeup of the trace element of high biological value by preparation in the plant that high-micro-element content is arranged, plant tissue or the cell.
At seed growth according to the present invention (germination) with during, a kind of solid or liquid nutrient medium have been used according to plant tissue of the present invention and cell cultures.
Preferred substratum is: White, Murashige-Skoog, Gamborg(B5) substratum and Nitsch type substratum (" Tissue Culture Methods and Applications ", Edited by Perul F.Kruse.JR.and M.K.Patterson, J.R., Academic Press, New York and London, 1973; Murashige, T.:Nutrition of Plant Cells and Organs in vitro.9,81-85,1973; B.V.Conger et al: " Cloning Agricultural Plants via in vitro Techniques ", CRC Press, 1981; P.H.Calcoot: " Continuous Cultures of Cells ", CRC Press, 1981).
Any element that can see in vivo and have biologic activity in a small amount all can be used as trace element and uses.Preferred trace element is selected from iron, zinc, copper, manganese, nickel, cobalt, molybdenum, selenium, chromium, iodine, fluorine, tin, silicon, vanadium, arsenic, boron, lithium, gold and silver, platinum, bromine, gallium, germanium, magnesium, calcium and aluminium.The unqualified character of cited element.
These trace elements are added in the substratum, to form organic or mineral compound.Under the situation of metal trace element, can preferably use its ionic compound, as other mineral acid salt, other covalent linkage compound such as ARSENIC TRI OXIDE 98 such as suitable fontanelle compound such as tin chloride, lithium chloride or gold trichloride, single nickel salt, chromium sulphate or Silver Nitrates, and organic compound such as seleno-cysteine.
In order to give trace element, can be fully or the pick of ground use the water of the natural origin that contains required trace element, as tap water, mineral water, thermal water, medical water or its mixture.Available above-mentioned water is as the solvent or the thinner of substratum.As the water of natural origin, preferably use Parad, Heviz and Margitsziget(Margaret Island, Budapest), the water of Hungary and P ǒ sty é n, Czechoslovakia, and the mineral water that is called Mohai Agnes.
An advantage of the water of natural origin is that they have constituted indivedual microelement compositions that form over a long time in nature at itself, they can detectedly have known organism to learn the active trace element (as seeing above-named various element) except that containing, and also containing in addition at present can't be micro-according to the unknown that prior art is analyzed out.Use natural water, just can make microelement composition and in fact exist but the activity of the trace element that is not detected is as yet utilized.Can be according to extra one or more elements that add to come in the natural microelement composition of enrichment of required method.
Germinate or the incubation period interocclusal record concentration range, plant grew well and its micronutrient levels are higher than natural value with this understanding.Also can select to be suitable for the plant that under high-micro-element content, grows, clone it then, in given high-micro-element concentration environment, further cultivate again, can make it in suspension culture, form one in case of necessity and organize or cell culture.
For making certain plants store trace element in a large number, can use the continuous domestication method, promptly not that the trace element with given concentration once adds, progressively increase but in 1-28 days time, the trace element of this concentration divided several times, until reaching desired level.In our experience, trace elements absorbed does not significantly increase after 28 days.
Usually this process just can be increased repeatedly the natural horizontal of trace element for 4-5 time.According to given trace element and the difference of plant, making and can making when beginning concentration in this way is 10 -9It is 10 that the natural horizontal of M reaches concentration -1The level of M.
Under aseptic condition, in having added micro-substratum, be better than 5-35 ℃ most and finish the rudiment process through 1-3 week.Be better than most under 10-30 ℃ the aseptic condition, finished the culturing process of plant tissue and cell through 7 to 28 days, can give 16 hours photoperiod in case of necessity.
Any in principle plant is all applicable to the present invention.But preferably use the plant that high-micro-element content is arranged that belongs to pulse family (Leguminosaesal), Papilionaceae (Fabaceae) and grass tree section (Graminales).
The plant that is suitable for comprises: Kidney bean (Phaseolus vulgaris), pea (Pisum sativum); root of Szemao crotalaria (Lens culinaris); the racemosus Radix Astragali (Astragalus racemosus); embrace the false Touch-me-notAction Plant (Neptunia amplexicaulis) of stem; white plumage French beans (Lupinus albus); wheat (Triticum aestivum); barley (Hordeum nodosum); rye (Secale cereale); oat (Avena sativa); rice (Oryze sativa); corn (Zea mays); millet (Panicum miliaceum) rye grass (Lolium perenne) and garlic (Allium sativum); onion (Allium cepa); Radix Raphani (Raphanus sativum); bamboo shoot (Lycopus exaltatus); borage (Borago officinalis); root of Redsepal Eveningprimrose (Oenothera biennis); soybean (Glycine soja); cowslip (Primulaveris); Herba Lathyri quinquenerii (Lathyrus sativus); Chinese sorghum (Sorghumvulgare).The all unqualified character of above-named plant.
By cloning process by above-mentioned under the high concentration trace elements condition well-grown plant entity preparation tissue or cell culture, they can absorb trace element in its body and as its constituent.After gathering, drying also stores the material that these have high biologic activity, to be used as additive, medicine and makeup.
Prepared according to the methods of the invention can be used after processing because of the vegetable material that has high-micro-element content to improve biological value.Can as required plumule be dissociated into its each integral part, promptly isolate root, stem and leaf, grind system and wear into fine particle dry, in advance then.Can directly use the powder that so makes.Under the situation of using-system or cell culture, can will wash with water after the culture drying removing excessive material as sugar and other substratum residue, dry then and process by the similarity method of handling plumule.
Drying process is preferably in temperature up to carrying out in 60 ℃ the airflow.The pre-system of grinding should make granular size reach below the 0.1mm.Smooth grinding then makes granular size reach 1 μ.
The powder that so makes is transformed into combination dosage form commonly used, as tablet, capsule, soft gel capsule agent, dragee, inhalation, granule, aerosol, syrup, emulsion and suspension agent, be used as food or medical composition by pharmaceutically acceptable non-toxic carrier or solvent.
The concentration of the plant material of high-micro-element content is about 0.5～90%(quality of total mixture), promptly this is to reach the necessary value of projected dose level.
Plant powder is mixed with a kind of solvent and/or carrier prepare these compositions, and can add as required, and make when using water as thinner, also can suitably select for use organic solvent as secondary solvent with emulsifying agent and/or dispersion agent.
Can use following secondary solvent; As water, nontoxic organic solvent, as paraffin (as oil fraction), vegetables oil (as hazelnut oil or sesame oil), alcohols is (as ethanol, glycerol), solid carrier, as the natural rock powder (as kaolin, clay, talcum, chalk), artificial inorganic powder (as the silicic acid or the silicate of high degree of dispersion), sugar is (as sucrose, lactose, glucose), emulsifying agent (as poly-/oxygen ethene/-fatty acid ester, poly-/oxygen ethene/-the aliphatics alcohol ester, alkyl sulfonic ester, aromatic yl sulphonate), dispersion agent is (as xylogen, sulphite-sewage base, methylcellulose gum, starch and poly-/V-Pyrol RC /) and slide the property material (as Magnesium Stearate, talcum, stearic acid and sodium laurylsulfonate).
The preferred modes of this material is oral.Certainly, except that above-mentioned carrier, also can contain other materials in the tablet of oral administration, as Trisodium Citrate, lime carbonate and Lin Suanergai and other assistant agents, as shallow lake Jia scold lemon spade astatine wait till vast cash residence of a high official  drink Gu Hangzhoupro eight clear  cast aside herd rare N shoot a glance at the rotten storehouse  Yan of kind  grain Nie moisture in the soil gown to the greatest extent the right  circle of ⑹   hazel  sled gu beer ⒒   herd rare T posthumous title ticket to tie up numerous   ┑ be that   ∫ He protects the handsome cape in   tall building and falls female  shackles fried twisted dough sticks  plutonium ape very sincere to send the funny appropriate  of the group gold-plating of the bright of argon south lot to sail an ancient type of banner hoisted on a featherdecked mast rare
When being used for makeup, can the plant powder of high-micro-element content be transformed into make-up composition by currently known methods.Preferably use following composition: body lotion, face-cream, hydration butterfat, regeneration butterfat, the butterfat of handling the eyes wrinkle, different gelifying agent and jelling agent, suntan lotion and shampoo, bathing foam and tooth powder (cream).
In order to prepare make-up composition, can use liquid vehicle such as water, alcohol (as ethanol and glycerine), paraffin oil, vegetables oil (as Oleum Helianthi), synthetic oil (as silicone oil), glyceryl ester (as the glycerine stearate monoesters) and fatty alcohol (as hexadecanol), can use emulsifying agent such as ion or nonionic emulsifier (as poly-/oxygen ethene/fatty acid ester in addition, poly-/oxygen ethene/aliphatics alcohol ester, alkyl sulfonic ester and aromatic yl sulphonate), gel former is (as alginate, the polyethylene carboxylicesters), and other additives, as the inhibitor that becomes sour (as fourth alkyl toluene), preservatives, essence or flavouring agent and tinting material.
Composition of the present invention also contains other known activeconstituentss except that containing plant powder.Can select the germ oil of physiologically active for use, as maize germ, wheat germ oil, Semen Cucurbitae oil, Oenothera oil, soybean oil and composition thereof.
The following example will be explained the present invention in more detail.
Embodiment 1
Make the Kidney bean (Phaseolus vulgaris) of using 3% disinfectant with hydrogen peroxide contain 0.5mM Ca SO 4Liquid on germinate, said liquid contain as the trace element cobalt chloride, its concentration is 10 -9～10 -1, cumulative successively.50 seeds were sprouted 10～14 days in every kind of concentration under 25 ℃.After reaching the highest cobalt concentration, show the Kidney bean well-grown.Plant embryos further grows Ye Hegen, and in the following clone's of aseptic condition.Root and leaf partly are grown on the solid white substratum of containing of improvement of above-mentioned the highest cobalt concentration.White substratum composed as follows: 0.36g/L sal epsom, 0.26g/L nitrocalcite, 0.2g/L sodium sulfate, 0.08g/L saltpetre, 0.065g/L Repone K, 0.165g/L SODIUM PHOSPHATE, MONOBASIC, the 3mg/L manganous sulfate, 0.5mg/L zinc sulfate, 0.5mg/L boric acid, 0.025mg/L copper sulfate, the 1mg/L ironic citrate, 7.5mg/L glycine, 1.15mg/L nicotinic acid, 0.25mg/L vitamin, 0.25mg/ L pyridoxine hydrochloride, 0.25ml calcium pantothenate, the 2mg/L iodoacetic acid, 20g/L sucrose, 8g/L agar.
20～30 ℃ of growths down, separated again once in every 14-21 days, per 16 hours period illumination are once.
Do not having the liquid Gamborg(B5 of agar) prepare suspension culture by leaf and root portion on the substratum, and make it to grow in the cultivation reactor of non-stop run.
Suitable incubation period (20～30 ℃, 14～21 days) is collected the plant part that contains a large amount of cobalts with centrifugal or filtration method later, decomposes, and use dry and preparation back.
Embodiment 2
Method by embodiment 1 is carried out, and different is all to use Gamborg(B5 under the various situations) solid medium.
Embodiment 3
The plant seed of using 3% disinfectant with hydrogen peroxide is germinateed, wherein at 8cm place tension one acidproof steel mesh in the vinyl disc of one 25 * 40 * 10cm.Institute's plant species subnumber of putting into is decided (50 to 250) according to its size, charges into aseptic liquid nutrient medium in the dish, reaches net place level.Liquid nutrient medium contains 0.5mM CaSO 4, and adding concentration is 10 -9～10 -1One or more trace elements of M, and/or reach 0～100% mineral water, thermal water, medical water or its mixture.Add once by per 1 to 5 day, in 1～21 day, make trace element reach final concentration.
At whole duration of germination, 16 hours being a periodicity of illumination with neon rayed seed, temperature: 5-35 ℃.
Sprouting the after drying plant and being milled to the spherolite size is 1 μ m.Vegetable cell is decomposed.
The gained flour can be directly used in the preparation composition.
Embodiment 4
Method according to embodiment 3.The germination plant is Kidney bean (Phaseolus vulgaris).Starting point concentration as micro-zinc sulfate is 1.8 * 10 -5M, final concentration are 1.8 * 10 -2M.
Embodiment 5
According to the method for embodiment 3 and 4, different is that add-on reaches 80% Parad(Hungary in the germination solvent) mineral water.
Embodiment 6
According to the method for embodiment 3～4, different is that the germination solvent is 100% Heviz(Hungary) medical water.
Embodiment 7
According to the method for embodiment 3～4, different is is 90% Margitsziget(Margaret Island with its amount, Budapest, and thermal water Hungary) is made the germination solvent.
Embodiment 8
According to the method for embodiment 3～4, the mineral water that different is is respectively 50% and 30% Hajduszoboszl ó (Hungary) mineral water and is called Mohai Agnes with its amount is made the germination solvent.
Embodiment minimum plant element initial concentration final concentration
9 Kidney bean ironic citrates 1.7 * 10 -5M 1.7 * 10 -2M
Copper sulfate 4 * 10 -7M 4 * 10 -3M
Zinc sulfate 1.8 * 10 -5M 1.8 * 10 -2M
10 Kidney bean single nickel salts 2 * 10 -8M 2 * 10 -3M
11 corn Manganous chloride tetrahydrates 1 * 10 -9M 6.2 * 10 -3M
12 oat Sodium orthomolybdates 3 * 10 -8M 3 * 10 -4M
13 rice Sodium orthomolybdates 3 * 10 -8M 3 * 10 -3M
Manganous chloride tetrahydrate 1 * 10 -9M 6 * 10 -3M
14 garlic Sodium Selenites 1 * 10 -9M 1 * 10 -2M
15 garlic Sodium Selenites 1 * 10 -9M 1 * 10 -2M
Germanium chloride 1 * 10 -9M 5 * 10 -2M
16 racemosus Radix Astragali germanium chlorides 1 * 10 -9M 5 * 10 -2M
17 armfuls of basic vacations contain germanium chloride 1 * 10 -9M 5 * 10 -2M
Shy grass
18 onion germanium chlorides 1 * 10 -9M 1 * 10 -5M
19 wheat water glass 1 * 10 -4M 1 * 10 -1M
20 rye grass Sodium Fluorides 1 * 10 -7M 2.8 * 10 -3M
21 bamboo shoot germanium chlorides 1 * 10 -9M 1 * 10 -5M
22 white plumage French beans germanium chlorides 1 * 10 -9M 1 * 10 -5M
23 Herba Lathyri quinquenerii germanium chlorides 1 * 10 -9M 1 * 10 -5M
24 Chinese sorghum Sodium orthomolybdates 3 * 10 -8M 3 * 10 -4M
25 barley single nickel salts 2 * 10 -6M 2 * 10 -6M
26 rye chromous sulfates 6.5 * 10 -9M 6.5 * 10 -4M
27 Radix Raphani platinum chlorides 1 * 10 -9M 1 * 10 -4M
28 Radix Raphani tin chlorides 1 * 10 -7M 9 * 10 -4M
29 barley ammonium vanadate 1 * 10 -9M 1 * 10 -5M
30 borage lithium chlorides 5 * 10 -5M 5 * 10 -2M
31 cowslip lithium chlorides 5 * 10 -5M 5 * 10 -2M
32 root of Redsepal Eveningprimrose lithium chlorides 5 * 10 -5M 5 * 10 -2M
33 pea gold trichlorides 3 * 10 -8M 1 * 10 -4M
34 pea Silver Nitrates 1 * 10 -9M 6 * 10 -5M
35 root of Szemao crotalaria gallium sulfates 2.3 * 10 -8M 2.3 * 10 -4M
36 pea germanium chlorides 1 * 10 -9M 1 * 10 -5M
37 soybean germanium chlorides 2 * 10 -5M 2 * 10 -2M
Calcium chloride 1 * 10 -5M 1 * 10 -2M
38 Kidney bean cobalt chloride * 6H 2O 4.2 * 10 -8M 4.2 * 10 -3M
39 Radix Dauci Sativae Sodium Selenites 1 * 10 -9M 1 * 10 -2M
40 Radix Dauci Sativae germanium chlorides 1 * 10 -9M 5 * 10 -2M
Embodiment 41
Make to carrying out leaf and root portion that cloning prepares and grow on the aseptic improvement white solid substratum.The component of white substratum is: 0.2g/L sodium sulfate, 0.08g/L vitriolate of tartar, 0.065g/L Repone K, 0.165g/L SODIUM PHOSPHATE, MONOBASIC, 3mg/L manganous sulfate, 0.5mg/L zinc sulfate, 0.5mg/L boric acid, 0.025mg/L copper sulfate, 1mg/L ironic citrate, 7.5mg/L glycine, 1.25mg/L nicotinic acid, 0.25mg/L vitamin, 0.25mg/L pyridoxine hydrochloride, 0.25mg/L calcium pantothenate, 2mg/L iodoacetic acid, 20g/L sucrose, 8g/L agar.Plant separated once down in 20-30 ℃ in per 14～21 days again, and carried out regular rayed in per 16 hours.After obtaining the tissue of appropriate amount, do not having the liquid Gamborg(B5 of agar) prepare suspension culture (Tissue culture Methods and Applications by leaf and root portion on the substratum, Edited by Perul F.Kruse, JR.and M.K.Patterson, J.R.Academic Press New York and Lodon, 1973; Murashige, T:Nutrition of Plant Cells and Organs in vitro 9,81-85,1973; Conger, B.V.etal:Cloning Agricultural Plants via in vitro Techniques CRC Press, 1981) and make it to grow in the cultivation reactor of non-stop run.
Incubation time is 10～30 ℃ to be descended 7～28 days, and periodicity of illumination is 16 hours.
By added in every 1-5 days once make liquid nutrient medium micronutrient levels 10 -9～10 -1Increase gradually between the M, until reaching final concentration, and/or make substratum contain 0～100% mineral water, thermal water, medical water or its mixture.
After finishing cultivation, through centrifugal or filter to collect the vegetable cell that contains a large amount of trace elements, with its decomposition, drying and prepare stand-by.
Embodiment 42
Stir and in the 200g lactose, add 20g silicic acid (trade(brand)name Aerosil) and adding 40g embodiment 38 active components of plants (it is a powder type, and humidity is 3%), homogenate under inert gas environment then down.
After wetting, mixture was stirred 30 minutes in kneader (spices device), until obtaining sticking with paste the sample agglomerate.Prepare particle with currently known methods by the gained agglomerate.
Under inert gas environment, at liquid dried device inner drying particle, below the water-content of sample is reduced to the measured desired value of Karl-Fischer method.Dried particle pulverized and charge into currently known methods in the hard gelatinum capsule of 250mg amount.Obtain 1,000 capsules and be used to alleviate scarce cobalt symptom.
Embodiment 43
The active components of plants of 40g embodiment 38 and 220g maize germ mixing homogenate, and charge in the oral capsule of 250mg in the soft gelatinum capsule shape apparatus for converting of a cold compaction by currently known methods.
Embodiment 44
The active components of plants of 40g embodiment 38 is with 140g lactose, 20g sucrose, 26g yam starch, 4g talcum and the homogenate of 4g Magnesium Stearate, makes particle by currently known methods then and is pressed into the tablet of 250mg.
Embodiment 45
By currently known methods in an elutriation pot elutriation by the tablet of embodiment 44 methods preparations and be coated with buffy layer.
Embodiment 46
10g is suspended in the 62.3g water by the active components of plants of embodiment 3-4 method preparation and is heated to 70～75 ℃, add then contain 7g hexadecanol, 10g maize germ, 2g Vaseline, 5g vaseline oil, 1g cera alba and 2.5g Tween 60 fat mutually in.
Stir emulsion until cooling.Add preservatives (0.1g) and spices (0.1g) down at 40 ℃.Obtain high-quality skin treating cream.
Embodiment 47
5g hexadecanol, 10g Vaseline, 4g vaseline oil and 2g Tween60 one arised under 70～75 ℃ dissolve, and the water that contains the 5.0g active components of plants that adds by embodiment 3-4 preparation reaches the 100%(quality).Stir gained solution until cooling, be respectively 0.1% and 0.15% preservatives and sweetener then in 40 ℃ of following addings.
Resulting composition is a kind of have high quality hydration and active body lotion of skin regeneration.
Embodiment 48
The active components of plants of 5g embodiment 3-4 is suspended in the Oenothera oil of 100g with the preservation of 0.02% butyl methylol.Then said composition being charged into 2ml becomes in the soft gelatinum capsule of tubular type.Press currently known methods and in soft gelatinum capsule shape apparatus for converting, prepare the peace bottle.
Embodiment 49
The activeconstituents of 5g embodiment 3-4 suspended in water and be added to 1g in 50g water among 24 hours the Carbopol of swelling 940.Wherein preservatives content is 0.1%.Mix two waters, add the 1.0g trolamine then and stir, up to forming gel.
So obtain a kind of have fabulous hydration performance, unruffled gel.
Embodiment 50
The 100g polyvinyl alcohol swelling of in 600ml distilled water, heating.This homogeneous solution is cooled to room temperature, adds 200ml concentration then and be the active components of plants that 96% ethanol and 10g are suspended in the embodiment 3-4 in the 190g water.Face skin wrinkle and the makeup of fine hydration performance are arranged so is eliminated.
Embodiment 51
In the active components of plants of 1g, add 93g Texapon N25, add the 10%(quality of 6ml then according to the method preparation of embodiment 3-4) hypagine M.Products obtained therefrom is used as hair mousse and shampoo.

Claims (10)

1, preparation has improved the plant of biological value or the method for its part, and other draws together being added with concentration is 10 -9～10 -1Culturing plants seed, tissue or cell in one or more micro-substratum of M.
2, according to the method for claim 1, it comprise use one or more down column elements as trace element: iron, zinc, copper, manganese, nickel, cobalt, molybdenum, selenium, chromium, iodine, fluorine, tin, silicon, vanadium, arsenic, boron, lithium, gold and silver, platinum, bromine, gallium, germanium, magnesium, calcium and aluminium.
3, according to the method for claim 1 to 2, it comprises the form of trace element with the organic or inorganic compound is added in the substratum.
4, according to the method for claim 1 to 2, it comprises the form of trace element with natural origin water, as the tap water, mineral water, thermal water, medical water or its mixture that contain required element partially or completely add in the substratum.
5, according to the method for claim 1 to 4, it is included in the substratum that contains a kind of or various trace elements that increases concentration gradually plant seed, tissue or cell cultures 1 to 28 day.
6, according to the method for claim 1 to 5, it comprises use Kidney bean (Phaseolus vulgaris), pea (Pisum sativum); root of Szemao crotalaria (Lens sulinaris); the racemosus Radix Astragali (Astragalus racemosus); embrace the false Touch-me-notAction Plant (Neptunia amplexicaulis) of stem; white plumage French beans (Lupinus albus); wheat (Triticum aestivum); barley (Hordeum nodosum); rye (Secale cereale); oat (Avena sativa); rice (Oryze sativa); corn (Zea mays); millet (Panicum miliaceum) rye grass (Lolium perenne); garlic (Allium sativum); onion (Allium cepa); Radix Raphani (Raphanus sativum); bamboo shoot (Lycopus exaltatus); borage (Borago officinalis); root of Redsepal Eveningprimrose (Oenotherea biennis); soybean (Glycine soja); cowslip (Primula veris); Herba Lathyri quinquenerii (Lcthyrus sativus); Chinese sorghum (Sorghum vulgare).
7, a kind of foodstuffs compositions that comprises by the plant that high-micro-element content is arranged, its part or its extract of claim 1 to 6 preparation.
8, the medical composition that comprises the plant that high-micro-element content is arranged, its part or its extract that prepare by claim 1 to 6.
9, the make-up composition that comprises the plant that high-micro-element content is arranged, its part or its extract that prepare by claim 1 to 6.
10, by the application of the plant that high-micro-element content is arranged, its part or its extract of claim 1 to 6 preparation with food, medicine or make-up composition form.
CN88108058A 1987-11-24 1988-11-23 Preparation and the application of the plant of biological value have been improved Pending CN1034020A (en)

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
HU5235/87 1987-11-24
HU523587A HU202913B (en) 1987-11-24 1987-11-24 Process for producing plant organs and tissue cultures with high biological value and enriched with trace elements, as well as dietary, cosmetic and pharmaceutical compositions comprising same
HU885141A HU202914B (en) 1988-10-05 1988-10-05 Process for producing plant embrionic culture of high biological value enriched in microelements and diethetic compositions containing them
HU5144/88 1988-10-05
HU885142A HUT53503A (en) 1988-10-05 1988-10-05 Method for producing plant emryo cultures of high biologic value enriched by tracer elements and pharmaceutical products containing same
HU5141/88 1988-10-05
HU5142/88 1988-10-05
HU5140/88 1988-10-05
HU514088A HU204186B (en) 1988-10-05 1988-10-05 Cosmetical composition containing plant-seed-cultures of high biological value enriched in microelements
HU5143/88 1988-10-05
HU5145/88 1988-10-05
HU885144A HUT53504A (en) 1988-10-05 1988-10-05 Method for producing plant tissue and embryo cultures of high biologic value enriched by tracer elements and dietetic products containing same
HU885145A HU202915B (en) 1988-10-05 1988-10-05 Process for producing plant tissue and celle cultures of high biological value enriched in microelements and pharmaceutical compositions containing them
HU514388A HU204187B (en) 1988-10-05 1988-10-05 Cosmetical composition containing plant tissue and celle culture of high biological value enriched in microelements

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WO (1) WO1989004599A1 (en)

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CN108064680A (en) * 2018-01-02 2018-05-25 广州汇圣森丰农业科技发展有限公司 A kind of black tomato ciltivating process rich in various trace elements

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ES2027518A6 (en) * 1990-12-18 1992-06-01 Andromaco Lab A process for preparing new non-covalent polysaccharide-protein associations having pharmacological activity.
FR2723848B1 (en) * 1994-08-31 1997-06-20 Oreal COSMETIC OR DERMATOLOGICAL COMPOSITION CONTAINING ENCAPSULATED PLANT EXTRACTS
ES2136580B1 (en) * 1998-05-05 2000-07-01 Provital S A PROCEDURE FOR THE PREPARATION OF A PHARMACEUTICAL AND / OR COSMETIC PRODUCT STIMULATING CUTANEOUS AND CAPILLARY AND MOISTURIZING METABOLISM, AND PRODUCT OBTAINED THROUGH SUCH PROCEDURE.
EP1262167A1 (en) * 2001-06-01 2002-12-04 Cognis France S.A. Cosmetic preparations containing an extract from germinating plants
JP2006199610A (en) * 2005-01-19 2006-08-03 Kao Corp Wrinkle-improving agent
KR100842360B1 (en) * 2006-09-22 2008-07-01 양옥열 Onion with high calcium concentration and cultivation method thereof
FR2915898B1 (en) * 2007-05-10 2009-06-12 Oreal PROCESS FOR THE PREPARATION OF ACTIVE INGREDIENTS ON THERMAL WATER AND COMPOSITIONS CONTAINING SAME
KR100900988B1 (en) * 2008-09-03 2009-06-04 의성흑마늘 영농조합법인 Preparation method for aged black garlic enriched selenium and zinc and extract using its
FR2978351B1 (en) * 2011-07-28 2014-02-21 Sederma Sa PLANT ORIGINAL MATERIAL, COMPOSITION CONTAINING SAME, AND TOPICAL COSMETIC USE
ITBO20110759A1 (en) * 2011-12-23 2013-06-24 I D Tech S R L COMPOSITION TO INCREASE THE CONTENT OF LITHIUM AND SELENIUM OF VEGETABLE AND OF THEIR PROCESSING PRODUCTS AND ITS USE

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CN108064680A (en) * 2018-01-02 2018-05-25 广州汇圣森丰农业科技发展有限公司 A kind of black tomato ciltivating process rich in various trace elements

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