HU202913B - Process for producing plant organs and tissue cultures with high biological value and enriched with trace elements, as well as dietary, cosmetic and pharmaceutical compositions comprising same - Google Patents

Abstract

Process for prepq. plants or parts of plants of improved biological value comprises growing plant seed, tissue or cells in a culture medium comprising trace element(s) in a concn. of 1 nM-0.1M. Also claimed are dietetic, pharmaceutical and cosmetic compsns. contg. plants, parts or extracts of high trace element content prepd. as above.

Description

FIELD OF THE INVENTION This invention relates to high biological value plant organs and tissue cultures enriched with trace elements and to dietetic, cosmetic and pharmaceutical compositions containing them.

Every living organism is made up of elements of a periodic table. Of these, 15 are found in large quantities in organisms such as C, Ο, N, S, etc., and 16 are only found in small quantities - these so-called trace elements - such as Se, Mn, Fe, Cu, Zn, B , etc.

In today 's increasingly polluted world, where agricultural production has reached unprecedented levels, despite the variety of fertilizers and nutrients, the amount of trace elements in the soil is decreasing.

As a result, disturbance occurs, the soil ....................... plant .................. .... in the animal or human food supply chain, which in turn leads to an increasing shortage of trace elements.

There are four main factors influencing the trace element content of plants:

(a) the genus, species;

(b) the type of soil on which the plant grows;

(c) climatic or seasonal conditions during the period of growth;

(d) the state of development of the plant.

These elements may decrease and grow or become permanent throughout the plant.

In the absence of a trace element, the growth of plants and the concentration of their elements are reduced.

In the next steps of the process, trace elements are reduced in human and animal organisms, which can cause a wide variety of symptoms. Deficiency of copper and zinc causes, among other things, hair loss, graying, skin lesions, and decreasing protein synthesis.

The deficiency of Si causes severe changes in the collagen, the cartilage tissue.

The deficiency of Mg and Mn also causes defective functioning of several enzymes, especially those synthesizing nucleic acid. Chromium deficiency disturbs the balance of lipid metabolism and glucose tolerance is reduced.

Selenium is an important part of glutathione peroxidase and through this free radical removal! integral part of reactions. (See detailed literature in Trace Elements in Human and Animated Nutrition; Erié J; Underwood Academic Press; New York, San Francisco, London, p. 56, p. 170, p. 196, p. 302, p. 398 1977).

From all these, it is clear how immense the importance of keeping the trace elements at an adequate level, and of compensating for their deficiencies, is for the living, primarily human body.

Algae are known to enrich the substances in their environment. Hungarian Patent Nos. 195,069 and 195,390 disclose a method of cultivating algae in a specific composition, whereby the algae take up useful substances in the medium, such as trace elements and vitamins, thereby producing biologically valuable plant mass.

Specifically, enrichment of germanium in algae is described in Swiss Patent No. 636,499.

However, it was not known that the trace element content of higher plants can also be influenced by culture in a defined composition.

It has been found that the composition and amount of trace elements accumulated in higher plant organisms can be influenced by culturing plant organs and tissues in a medium containing a specific composition and amount of trace elements. It has also been discovered that the nutrient content of the nutrient medium is incorporated into higher plants in a form, such as a complex or protein-bound form, that is biologically and biochemically processed more by the human body than in lower plants such as algae and fungi. microelements. Finally, it has been discovered that the trace element content of certain natural waters, particularly beneficial in composition and quantity to humans, can be continuously introduced into higher plants and thus directly utilized.

The invention therefore trace elements procedure enriched high biological value of plant organs and cloned plant tissue cultures thereof, so that fresh water, mineral water, spring water, medicinal water and / or mixtures and / or assembled nutritive salts medium 10 ^'9-10' thereof of the invention ¹ M concentration of trace elements enriched, then sterilized under pressure at 121 'Con for 20 minutes or by flow through a sterile filter in a known manner and inoculated with plant organs or cloned plant tissue culture accustomed to the trace element or trace elements in question and cultured at 20-30 ° C for 7-28 days with 16 applied, and then the plant parts enriched with the trace elements are separated, dried and disrupted by ultrasound or milling in a manner known per se.

It is surprising that higher plants incorporate the micro-nutrient content of the environment into their bodies, just as inferior plants. The micronutrient to be enriched can easily be added to a medium of complex composition required for the cultivation of higher plants, differentiated by plant species and often by plant part. The micronutrient does not interfere with the medium and does not affect the culture conditions.

Particularly surprising is the finding that some parts of higher plants utilize a given micronutrient better than the whole or other parts of the plant, and therefore only the particular plant part is cultivated. By way of example, the root of a carrot incorporates about three times as much selenium as its leaf.

Following direct extraction from plant organs, plant tissues or cells with a high content of trace elements, dietary products, pharmaceuticals or cosmetics containing the trace elements in high biological value are prepared.

In the cultivation of plant organs, tissues and cells according to the invention, the medium used is solid fish-medium or liquid medium. Preferred media are White, MurashigeSkoog, Gamborg (B5) and Nitsch (Tissue Culture Methods and Applications), edited by Peruvian F. Kruse, J. and K. Patterson, J. J. Acedemic Press, New York and London, 1973. Murashige, T .: Nutrition of Plant Cells and Organs in Vitro, 9, 81-85, 1973, Conger BV, et al., Agricultural Plants via In Vitro Techniques, CRC Press, 1981, Calcott PH: Continuous Cultures of Cells, CRC Press, 1981).

Trace elements may be any biologically active element that is present in small quantities in the living organism. Preferred representatives: iron, zinc, copper, manganese, nickel, cobalt, molybdenum, selenium, chromium, iodine, fluorine, tin, silicon, vanadium, arsenic, leather, lithium, gold, silver, platinum, bromine, gallium, germanium, magnesium , calcium and aluminum. The list is not restrictive ·

The trace elements are introduced into the medium in the form of an organic or inorganic compound. Suitable halides such as tin chloride, lithium chloride or gold chloride, other mineral acids such as nickel sulfate, chromium sulfate or silver nitrate are preferred for ionic compounds such as metallic trace elements, and covalently bonded compounds such as arsenic trioxide; and organic compounds such as selenocysteine.

For the addition of the trace elements, water of natural origin, such as fresh water, mineral water, thermal water, medicinal water or a mixture thereof containing the desired trace element, may be used in whole or in part. The water may also be used as a solvent or diluent for the medium used. Naturally occurring waters, such as those of Párád, Hévíz and Margaret Island, as well as the waters of Piestany (Czechoslovakia) and the mineral water Ágnes Mohai are preferred.

The great advantage of naturally occurring waters is that they have a unique trace element composition that has evolved spontaneously over a long period of time, and in addition to the currently detectable and known biological trace elements (see list above), additional, yet unknown and state of the art they contain trace elements which cannot be analyzed. Thus, by using natural waters we can take advantage of the favorable trace element composition and the effect of naturally occurring but not yet investigated trace elements. One or more elements of the natural trace element composition may be enriched by separate addition to the extent desired.

During cultivation, you record the concentration range where the plants are still developing well and their trace element content is higher than their natural value. Plants or parts of plants that are capable of developing at high levels of trace elements are selected, cloned, further cultivated at a given high level of trace elements and then further grown in tissue or cell culture, preferably in suspension culture.

In some cases, one or another part of the plant accumulates a given trace element more than the whole or other parts of the plant. For example, carrot root enriches about three times as much selenium as its leaf. In this case, these parts of the plant are cultivated.

The process generally increases the natural level of trace elements by 4-5 orders of magnitude. This particularly means that the natural level 10 'starting from ⁹ M depending on the concentration of that crop and nyomelemtől extent approaching 10' ¹ M concentration.

Cultivation of plant tissues or cells is preferably carried out for 7-28 days at 10-30 ° C under sterile conditions, if necessary with 16-hour illumination periods.

In principle, any plant can be used in our process. Preference is given in particular to plants containing high levels of trace elements of legumes (Leguminosae), butterflies (Fabaceae), lawns (Graminales).

Examples of useful plants include: beans (Phaseolus vulgaris), peas (Pisum sativum), lentils (Lens culinaris), grass (Astragalus racemosus), Neptunia amplexicaulis, white lupine (Lipinus albus), wheat (Trit) aestivum), barley (Hordeum nodosum), rye (Secale cereale), oat (Avena sativa), rice (Oryza sativa), corn (Zea mays), millet (Panicum miliaceum), English perch (Lolium) perenne), as well as garlic (Album sativum, onion (Album cepa), alfalfa (Raphanus sativus), venison (Lycopus exaltatus), borage (Borago officinabs), evening primrose (Oenothera biennis), soybean (Glycine) salt), primrose (Prirqula veris), nail ledge (Lathyrus sativus), ciji (Sorghum vulgare), but this list is not limiting.

Thus, these plants can be cloned to produce highly resistant individuals or tissue or cell cultures that grow well with high concentrations of trace elements, and are incorporated, incorporated, harvested, dried and digested as high-quality nutritional supplements, they can be used for medicine and cosmetics.

Plant material of improved biological value, i.e. high in trace elements, obtained by the process of the invention is processed for use. The culture is filtered, and excess material such as sugar and other media is discarded by washing with water, dried, milled and micronized. The powder thus obtained can be used directly. The drying is preferably carried out with flowing air at a temperature of up to 60 ° C. Pre-milling is carried out to a particle size of less than 0.1 mm and micronization to a particle size of 1 micron.

The resulting powder for use as a dietary or pharmaceutical preparation can be formulated into conventional, non-toxic, pharmaceutically acceptable, non-toxic, or pharmaceutically acceptable excipients, such as tablets, capsules, soft gelatine capsules, dragees, pills, granules, aerosols, syrups, emulsions or suspensions. THE

The concentration of the plant material having a high trace element content is about 0.5-90% by weight of the total mixture, that is, a value which is necessary to achieve the desired dosage level.

The compositions may be prepared, for example, by mixing the plant powder with a solvent and / or carrier, optionally using emulsifying and / or dispersing agents. When water is used as a diluent, organic solvents may optionally be used as co-solvents.

Suitable excipients are, for example, water, non-toxic organic solvents such as paraffins (e.g. petroleum fractions), vegetable oils (e.g. peanut or sesame oil), alcohols (e.g. ethanol, glycerol), solid carriers such as natural rock flour (e.g. kaolin, clay, talc, chalk), artificial inorganic powders (e.g., highly dispersed silica or silicates), sugars (e.g., cane, lactic and glucose), emulsifiers (e.g., polyoxyethylene) fatty acid ester, poly ( oxyester ester fatty alcohol ether, alkylsulfonate, arylsulfonate), dispersants (e.g., lignin, sulfite-lye, methylcellulose, starch and polyvinylpyrrolidone) and lubricants (e.g., magnesium stearate, talc, stearic acid). and sodium lauryl sulfate).

The administration is carried out in the usual manner, preferably orally. For oral administration, the tablet may, of course, also contain, in addition to the carriers listed above, other substances such as sodium citrate, calcium carbonate and dicalcium phosphate, together with other additives such as starch, preferably potato starch, gelatin and the like. Lubricating agents such as magnesium stearate, sodium lauryl sulfate and talc may also be used for tabletting purposes. In the case of an aqueous suspension for oral use, the active compounds may be mixed with various flavors and coloring agents in addition to those listed above.

For use in cosmetics, a high trace element plant powder is converted into standard cosmetic formulations in a manner known per se. Preferred formulations include lotions, face creams, moisturizing creams, regenerating creams, eye creams, various gels and gels, masks, and shampoos, foam baths and dentifrices.

Liquid carriers such as water, alcohol (e.g. ethanol and glycerol), paraffin oil, vegetable oils (e.g., sunflower oil), synthetic oils (e.g., silicon oil), glycerol esters (e.g., glycerol monostearate) may be used in the preparation of cosmetic compositions. ) and fatty alcohols (e.g. cetyl alcohol) and emulsifiers such as ionic or nonionic emulsifiers (e.g. polyoxyethylene fatty acid ester, polyoxyethylene fatty alcohol ether, alkyl sulfonate and aryl sulfonate), gelling agents. (e.g., alginates, polyvinyl carboxylic acid), and other additives such as anti-fouling agents (such as butylhydroxytoluene), preservatives, perfumes or colorings and coloring agents.

The formulations may contain other known active ingredients in addition to the plant powder of the present invention. Particularly beneficial effects can be achieved with the use of germ oils with physiological effects. Cold pressed vegetable oils such as corn germ oil, wheat germ oil, pumpkin seed oil, evening primrose oil, soybean oil, and mixtures thereof are preferred.

The invention is further illustrated by the following examples. Following the example, the level of trace element content achieved is given, where the value in parentheses represents the trace element content of the control plant grown in medium without trace element.

Example 1

Bean grains (Phaseolus vulgaris) disinfected with 3% hydrogen peroxide are germinated in a liquid containing 0.5 mM CaSO4, which also contains cobalt chloride in order of magnitude up to a concentration of 10% to ^100% . Germination was performed with 50 seeds at 25 ° C for 14 days. Subsequently, the highest concentration of cobalt that the bean still has good growth (4.2 x 10 ³ M) was determined and the leaves and roots of the seedlings grown here were further cloned under sterile conditions. Root and leaf fragments are grown on modified solid White medium supplemented with the most appropriate concentration of cobalt already established. The composition of the white medium is as follows: 0.36 g / l magnesium sulfate, 0.26 g / l calcium nitrate, 0.2 g / l sodium sulfate, 0.08 g / l potassium nitrate, 0.065 g / l 1 potassium chloride, 0.165 g / l sodium hydrogen phosphate, mg / l manganese sulfate, 05 mg / l zinc sulfate, 0.5 mg ^z l boric acid, 0.025 mg / l copper sulfate, 1 mg / l iron citrate,

7.5 mg / l glycine, 1.25 mg / l niacin, 0.25 mg / l thiamine HCl, 0.25 mg / l pyridoxine HCl, 0.25 ml calcium pantothenate, 2 mg / l indole acetic acid, 20 g / l sucrose, 8 g / l agar.

Growth was performed at 28 'C, illumination was applied at 16 hour intervals.

Leaf and root particles were slurried on liquid Gamborg (B5) medium without agar and grown in a continuous culture reactor at 22 ° C for 28 days. Cobalt-rich plant parts are collected by centrifugation or filtration, digested, dried and prepared for use.

Example 2

All were carried out as in Example 1, except that in each case Gamborg (B5) medium was used. As a result, the Co content of the bean is increased from a micronized air-dried root histology tissue suspension to 460 pg / g (180 pg / g).

Example 3

All were carried out as in Example 1, except that 50% of Paradise mineral water was added to the germination solution and the medium instead of the distilled water. The plant material takes over the composition of the water trace element used.

HU 202 913 Β

Example 4

In all cases, the same procedure as in Example 1 was used, except that 100% water of thermal water was used as a germination solution and this medium was also used instead of distilled water. The plant material takes over the composition of the water trace element used.

Example 5

In all cases, the procedure of Example 1 was followed, except that 100% Margaret Island thermal water was used as a germination solution, and this medium was also used in place of distilled water. The plant material takes over the composition of the water trace element used.

Example 6

In all cases, the same procedure as in Example 1 was followed, except that 50% of Hajdúszoboszló's healing water and 50% of Ágnes Mohai's mineral water were used as germination solutions, and this mixture was also used instead of distilled water. The plant material takes over the composition of the water trace element used.

Example 7

1 and 3-6. Example 1, except that the concentration of iron, copper and zinc is increased by 1.7 · 10 ′ ² M iron citrate, 4 · 10 ′ ³ M copper sulfate and 1.8 · 10 ′. ^{In the form of 2} M zinc sulfate. Achieved micronized air-dry root meristem tissue suspension has a trace element content of 400 pg / g (80 pg / g) Fe, 420 pg / g (10 pg / g) Cu and 210 pg / g (44 Pg / g) Zn.

Example 8

1 and 3-6. A procedure according to one example above, with the difference that 2 · using 10 ^'3 M nickel sulfate medium enriched. Result Bean Root Meristem Tissue Suspension (Air Dry): 2100 pg / g (0.4 pg / g) Ni.

Example 9

1 and 3-6. A procedure according to one example above, with the difference that the used corn (Zea mays), instead of bean and the manganese concentration is raised to 6.2 · 10 ^"3 M manganese chloride addition. Germination temperature at 22 'C for 21 days, tissue culture leaf, shoot from meristem for 21 days at 22' C. Result in micronized air-dry tissue suspension: 720 pg / g (13.0 pg / g) Mn.

Example 70

1 and 3-6. The procedure of any one of Examples 1 to 4 is followed, except that oats (Avena sativa) are used instead of beans and the amount of molybdenum in the form of 3 x 10 ⁴ Μ of sodium molybdenate is increased. Germination was carried out at 24 ° C for 14 days, and tissue cultivation of leaf callus stem meristem at 18 ° C for 28 days. Result in micronized air-dry tissue suspension: 240 pg / g (0.2 pg / g) Mo.

Example 77

1 and 3-6. The procedure of any one of Examples 1 to 4, except that rice (Oryza sativa) is used in place of beans and the molybdenum, manganese concentration is increased in the form of 3 x 10 ³ M sodium molybdenate and 6 x 10 ³ M manganese chloride. Germination at 35 ° C for 14 days, tissue culture from root meristem callus at 30 ° C for 18 days. Result in micronized air-dry tissue suspension: 360 pg / g (0.4 pg / g) Mo and 710 pg / g (15.0 pg / g) Mn.

Example 72

1 and 3-6. A procedure according to one example, except that used as garlic (Allium sativum), instead of bean, and the Se concentration was increased form 10 ^'2 M sodium selenite. Germination at 24 'C for 16 days, tissue culture shoot, leaf meristem callus at 21' C for 21 days. Result in micronized lysate tissue suspension: 80 pg / g (<0.01 pg / g) Se.

Example 73

In all cases, the procedure of Example 12 was followed except that the plant was Astragalus racemosus. Result in micronized air-dry tissue suspension: 180 pg / g (<0.01 pg / g) Se.

Example 14

In all, the procedure of Example 13 was followed, except that Neptunia amplexicaulis was used as the plant. Result in micronized air-dry tissue suspension: 120 pg / g (0.02 pg / g) Se.

Example 75

1 and 3-6. The procedure of any one of Examples 1 to 4, except that the onion (Allium cepa) is used as a plant and the selenium concentration is increased, in the form of selenocysteine. Germination at 24 'C for 14 days, tissue culture from root meristem callus at 22' C for 18 days. Result: micronized air-dry tissue suspension: 80 pg / g (<0.01 pg / g) Se.

7o. example

1 and 3-6. The procedure of any one of Examples 1 to 4 was followed, except that wheat (Triticum aestivum) was used in place of beans and that the silicon concentration was increased to 10 ¹ M sodium silicate. Germination at 18 'C for 21 days, tissue culture shoot, leaf from meristematic callus at 24' C for 21 days. Result in micronized air-dry tissue suspension: 24000 pg / g (12000 pg / g) SI.

Example 77

1 and 3-6. Example 1, except that the English duckweed (Lolium perenne) and the fluorine

GB 202,913 Β concentration is raised in the form of 2.8 · 10 ^"3 M sodium fluoride. Germination at 26 ° C for 14 days, tissue culture leaf, shoot meristem stem callus at 24 ° C for 21 days. Result in micronized air-dry tissue suspension: 380 pg / g (40.0 pg / g) F.

Example 18

All were treated in the same manner as in Example 17 except that the plant was used as Lycopus exaltatus. Result: 450 pg / g (28.0 pg / g) F.

Example 19

In all, the procedure of Example 17 was followed except that the white lupine (Lupinus albus) was used as the plant. Result: 380 pg / g (50.0 pg / g) E

Example 20

In all, the procedure of Example 17 was followed except that the nail ledger (Lathyrus sativus) was used as the plant. Result: 720 pg / g (135 pg / g) F.

Example 21

In all, the procedure of Example 10 was followed except that the zircon (Sorghum vulgare) was used as the plant. Result: 110 pg / g (0.3 pg / g) Mo.

Example 22

1 and 3-6. The procedure of any one of Examples 1 to 4, except that barley (Hordeum nodosum) is used as a plant and the nickel concentration is increased in the form of 2 x 10 ³ M nickel sulfate. Germination at 15 'C for 24 days, tissue culture shoot, callus from leaf meristem at 26' C for 18 days. Result in micronized air-dry tissue suspension: 900 pg / g (0.5 pg / g) Ni.

Example 23

1 and 3-6. The procedure of any one of Examples 1 to 4 was followed except that the plant was used as a plant rye (Secale cereale) and the chromium concentration was increased in the form of 6.5 x 10 ⁴ M chromium sulfate. Germination at 20 'C for 21 days, tissue culture shoot, callus from leaf meristem at 26' C for 21 days. Result in micronized, air-dry tissue suspension: 500 pg / g (8.0 pg / g) Cr.

Example 24

1 and 3-6. The procedure of any one of Examples 1 to 8 was followed except that radish (Raphanus sativus) was used as a plant and the platinum concentration was increased in the form of 10 ⁴ M platinum chloride. Germination at 28 'C for 18 days Growth of tissue from root meristematic callus at 28' C for 21 days. Result in micronized, air-dry tissue suspension:

980 pg / g (1.5 pg / g) Pt.

Example 25

In each case, the procedure of Example 24 was followed except that maize (Zea mays) was used as the plant. Result in micronized, air-dry tissue suspension: 500 pg / g (<0.1 pg / g).

Example 26

Everything in Figures 1 and 3-6. The procedure of any one of Examples 1 to 8 was followed except that radish (Raphanus sativus) was used as a plant and the tin concentration was increased in the form of 9 · 10 ' ⁴ M tin chloride. Germination at 21 'C for 18 days, tissue culture from root meristematic callus at 28' C for 21 days. Result in micronized air-dry tissue suspension: 280.0 pg / g (<1.0 pg / g) Sn.

Example 27

1 and 3-6. The procedure of any one of Examples 1 to 8, except that barley (Hordeum nodosum) is used as the plant, and the vanadium concentration is increased in the form of 10 ⁵ M ammonium vanadate. Germination at 24 'C for 16 days, tissue culture from root meristem callus at 23' C for 19 days. Result in micronized air-dry tissue suspension: 1540 pg / g (17.0 pg / g) V.

Example 28

1 and 3-6. The procedure of any one of Examples 1 to 4 is followed, except that the plant is rye (Secale cereale) and the concentration of arsenic in the form of arsenic trioxide is increased. Germination at 18 'C for 16 days, tissue culture shoot, leaf meristem stem callus at 26' C for 21 days. Result in micronized air-dry tissue suspension: 250 pg / g (2.0 pg / g) As.

Example 29

1 and 3-6. The procedure of any one of Examples 1 to 8 was followed except that the borage (Borago officinalis) was used as a plant and the concentration of lithium was raised in the form of 5.10 l ² M lithium chloride. Germination at 21 'C for 21 days, tissue culture from root meristematic callus at 22' C for 24 days. Result: 6900 pg / g (32.0 pg / g) Li.

Example 30

In all, the procedure of Example 29 was followed except that primrose (Primula veris) was used as the plant. Result: 6400 pg / g (19.0 pg / g) Li.

Example 31

In each case, the procedure of Example 30 was followed except that the primrose (Oenothera biennis) was used as the plant. Result: 7000 pg / g (20.0 pg / g) Li.

Example 32

1 and 3-6. except that pea (Pisum sativum) was used as the plant and the gold con-61

The HU 202 913 Β concentration is increased in the form of 3 · 10 ′ ⁴ M gold chloride. Germination at 26 ° C for 18 days, tissue culture from root meristem callus at 28 ° C for 22 days. Result in micronized air-dry tissue suspension: 720 pg / g (1.0 pg / g) Au.

Example 33

All proceed as in Example 32, except that the silver concentration is increased in the form of 6 x 10 ⁵ M silver nitrate.

Result: 1500 pg / g (10.0 pg / g) Ag.

Example 34

1 and 3-6. The procedure of any one of Examples 1 to 8, except that the lens (Lens culinaris) is used as a plant, and the concentration of gallium in the form of 2.3 x 10 ⁴ M gallium sulfate is increased. Germination at 20 'C for 21 days, tissue culture from root meristem callus at 20' C for 28 days. Result in micronized air dry tissue suspension: 440.0 pg / g (0.0007 pg / g) Ga.

Example 35

The procedure of Example 34, except that the germanium concentration was increased form 10 ^"5M germanium tetrachloride. Result: 410.0 pg / g (0.04 pg / g) Ge.

Example 36

1 and 3-6. The procedure of any one of Examples 1 to 4, except that the soybean (Glycine salt) is used as a plant, and the magnesium and calcium concentrations are increased in the form of 2 x 10 ² M magnesium chloride and 10 ² M calcium chloride. Germination at 21 'C for 14 days, tissue culture from root meristem callus at 30' C for 7 days. Result: 10,800 pg / g (1800 pg / g) Mg and 14,400 pg / g (2,000 pg / g) Ca.

Example 37

All proceed as in Example 36 except that the concentrations of zinc, manganese and selenium in the form of zinc sulfate, manganese sulfate and selenocysteine are increased. Result in micronized, air dry tissue suspension: 100 pg / g (20.0 pg / g) Zn, 380 pg / g (15 pg / g) Mn and 80 pg / g (<0.01 pg / g) Se.

Example 38

1 and 3-6. The method of any one of Examples 1 to 4 is followed except that the carrot (Daucus carota) is used as the plant. Selenium was added as trace element in the form of 10 ² M sodium selenite. Germination at 22 'C for 21 days. Result for complete micronized, air-dry plant: 350 pg / g (0.3 pg / g) Se.

Example 39

All proceed as in Example 38, except that the carrot leaf, shoot meristem callus and root meristem callus are cultivated separately. Result: leaf - 550 pg / g, root - 1600 pg / g Se.

Example 40

In each case, the procedure of Example 39 was followed except that Silibum was used as the plant. Result: leaf - 400 pg / g, root - 800 pg / g Se.

Example 41

In all cases, Example 39 was followed except that Safrol was used as the plant. Result: leaf - 51 pg / g, root - 237 pg / g Se.

Example 42

In each case, the procedure of Example 40 was followed except that zinc was used as the trace element in the form of 1.8 · 10 ² M zinc sulfate. Result: leaf - 13,600 pg / g, root - 2600 pg / g Zn.

Example 43

One proceeds according to Example 41, except that the trace element zinc · using 1.8 · 10 ^"2 M zinc sulfate form. Result: leaf - 900 pg / g, root - 280 pg / g Zn.

Example 44

Tabettázás

40 g of the air-dried, micronized plant active ingredient are mixed with 140 g of lactose, 20 g of sucrose, 26 g of potato starch, 4 g of magnesium stearate and 4 g of VI. After the homogenization, the powder mixture is sieved through a V screen, then mixed with a 2% aqueous gelatin solution and granulated through a 1.6 or 2.0 mm sieve, dried in a tray and mechanically (lab-size). Korsch eccentric tabletting machine) into 250 mg tablets.

dragee

The tablets obtained are coated with a sugar coating in an Erweka dragee pan. A 60% by weight sugar solution and a calcium carbonate primer powder are used to apply the base coat 10. Coloring In small portions, the dragees are polished with yellow food coloring and finally with white wax.

Claims (7)

CLAIMS 1. A process for the production of high biological value plant organs and cloned plant tissue cultures enriched with trace elements comprising the step of 10 ' ^{9 to} 10' ¹ M containing nutrients composed of fresh water, mineral water, thermal water, medicinal water and / or mixtures thereof and / or nutrients. then pressurize at 121 ° C for 20 minutes. · Sterilize by passing through a sterile strainer and using the plant organs accustomed to the nutrient-enriching trace element or elements. Inoculation is carried out by seeding with cloned plant tissue culture and culturing at 20-30 'for 7-28 days, if necessary, with 16-hour illumination periods, and then separating the trace element-enriched plant parts, if necessary by counting by ultrasound or we grind it by grinding. 2. Method according to claim 1, characterized in that the medium is the following trace elements comprise one or more inorganic or organic compounds thereof 10 is fortified with 10 ^'9 -10 ^{with 1} M concentration of iron, zinc, copper, manganese, nickel, cobalt, molybdenum , selenium, chromium, iodine, fluorine, tin, silicon, vanadium, arsenic, boron, lithium, gold, silver, platinum, bromine, gallium, germanium, magnesium, calcium, aluminum. 15 A process according to claim 1 or 2, characterized in that, with selenium, garlic (Allium sativum), onion (Allium cepa), common grass (Astragalus racemosus), Neptunia amplexicaulis, borage (Borago officinalis), evening primrose (Oenothera biennis) ), 20 primrose (Primula veris) are enriched. Process according to claim 1 or 2, characterized in that cobalt, nickel, iron, copper, zinc, lithium, beans (Phaseolus vulgaris), peas (Pisum sativum), soy (Glycine salt), radish (Raphanus sativus) ), white starfurters (Lupinus albus), nail ledges (Lathyrus sativus). Process according to claim 1 or 2, characterized in that arsenic, vanadium, manganese, molybdenum, silicon, circus (Sorghum vulgare), wheat (Triticum aestivum), barley (Hordeum nodosum), rye (Secale cereale), oats (Avena sativa), maize (Zea mays), rice (Oryza sativa). 6. A process according to claim 1 or 2, wherein the iodine, fluorine, bromine is enriched with English larvae (Lolium perenne) and with ferrets (Lycopus exaltatus). 7. Dietary procedure; for the preparation of a cosmetic or pharmaceutical composition, characterized in that a high biological value plant material enriched with trace elements according to claim 1 is admixed with a cosmetic or pharmaceutical carrier and one or more other cosmetic or pharmaceutical excipients, and the mixture is formulated for administration.