CN103397066A - Method for extracting active peptides from Qinzhou yellow pond turtle - Google Patents

Abstract

The invention discloses a method for extracting active peptides from a Qinzhou yellow pond turtle. The method comprises the following steps of raw material treatment, hydrolysis, enzymolysis, freezing, melting, concentrating and spray drying to obtain a finished product. Compared with the traditional seed solution shaking table activation, inoculation, fermentation liquid culture and wall breaking technologies, the method has the advantage that the time is greatly shortened.

Description

From the stone small water turtle of Qiezhou, extracting the method for bioactive peptide

Technical field

The present invention relates to a kind of preparation method of bioactive peptide, be specifically related to a kind of from the stone small water turtle of Qiezhou, extracting the method for bioactive peptide.

Background technology

The stone small water turtle, claim again the stone tortoise, intends Clemmys, belongs to land and water amphibian, and high pharmaceutical use, edibleness, ornamental value and Humanistic Value are arranged.Well known Chelonian is the thing of longevity.The life-span of tortoise reaches the centuries, and as the saying goes " cuckold in thousand, 10000 years tortoises ", tortoise not only has extremely strong vitality, and the polypeptide longevity factor is arranged in body.Edible, medicinal tortoise meat can be promoted longevity, and the polypeptide longevity factor in long-lived tortoise body can fully be absorbed by human body.The a lot of active substances of human body are all that the form with peptide exists, and what finder's soma was heavy after deliberation is all protein (just being called protein by 50 peptides that form with upper amino acid) more than 70%, there is no thus peptide, just there is no activity, just there is no life.Peptide relates to each field of hormone, nerve, Growth of Cells and reproduction of human body.Growth, growth, immunomodulatory and the metabolism of bioactive peptide major control human body, it is in a kind of equilibrium state at human body, after if bioactive peptide reduces, the function generation important change of human body, for children, his growth, growth become slowly, even stop, and go down for a long time just to have formed the dwarf; To grownup or the elderly, after lacking bioactive peptide, the immunizing power of self will descend, metabolic disturbance, and endocrine disturbance, cause the generation of various diseases, as insomnia, health is become thin or edema.Because bioactive peptide also acts on neural system, thus the human body slow movement that will become, brains is no longer wise, and main is that bioactive peptide reduces, and causes that directly each position of the person engenders comprehensive aging, causes various diseases.The scientific research personnel also finds never cancer stricken of tortoise, and its body contains cancer-resisting substance.Use the tortoise elite, make the promote longevity forward position target of this life science of people, current noticeable.

Qiezhou is located in coastal, and advantageous natural advantage and the conventional industries advantage of cultivation testudinate arranged, and because geographical environment is different with water and soil, the testudinate meat that produce in Qiezhou is fragrant and sweet especially, enjoys the great reputation of " the name tortoise goes out Qiezhou " all over the world.Qiezhou stone small water turtle, be Qinzhou City, Guangxi Zhuang Autonomous Region special product, is the Chinese geography famous special product.Qiezhou stone small water turtle culture has long history, accumulated ripe experience, the raiser utilizes the abundant advantage of local coastal, riverine low-value small fish, take the live fish and shrimp natural feed, feeds as main, the stone small water turtle of cultivation, more near the natural, ecological product, has higher nutritive value.Through development for many years, family more than 10,000 is developed at the stone small water turtle culture family in Qiezhou, and cultivation quantity develops into more than 70 ten thousand, and more than 300,000,000 yuan of annual value of production, testudinate industry have become the mass peasants of Qinzhou City and got rich to the mainstay industry of health.

As CN200910061718.0 technique for preparing skin longevity factor peptide, a kind of technique for preparing skin longevity factor peptide is disclosed, by seed liquor shaking table activation 15-30 hour, then be inoculated in the fermented liquid that contains the PTS polypeptide.Fermented liquid 15-40 ℃ of gap stir culture 1-4 days, then by fermented liquid boiling water bath broken wall 10-40 minute, centrifugal collection supernatant liquor, obtain protein content to its concentrate drying high, the skin longevity factor skin that functionally active is good, but the time is long, and yield is low.How in the stone small water turtle body of Qiezhou, to extract effective bioactive peptide, use the modern high technology Biotechnology, be developed into high-end protective foods and make the mankind improve immunizing power, build up resistance, make the mankind more long-lived, live more healthyly, more excellent.The research and development of this project has huge market potential and development prospect, how to utilize better at present enzymatic reaction, use the biochemical manufacturing technology of modern high technology, developing better extraction separation and purification technical matters, to make extraction yield obtain improving constantly be the direction of research.The Research Prospects in this field is extensive, has very large exploiting potentialities.The exploitation of protective foods is used and is significant.

Summary of the invention

The invention provides a kind of method of bioactive peptide of extracting take Qiezhou stone small water turtle as raw material that technique is simple, efficiency is high.

The present invention is achieved through the following technical solutions:

A kind of is the method that raw material extracts bioactive peptide from Qiezhou stone small water turtle, comprises the steps:

A. raw material is processed: get Qiezhou stone small water turtle, after gilling, clean up, keep turtle shell, tortoise meat;

B. hydrolysis: the pure water that adds 8-10 doubly to measure, with 1M HCl, regulate mixture pH value to 1.8-2.5, after boiling, remain pressure 0.1Mpa hydrolysis 8-10 hour, boil to remain in press process and note supplementing the moisture that loses;

C. enzymolysis: after hydrolysis completes, filter, collect filtrate, add 1M NaOH adjust pH to 3.5-5.5, add 0.2-0.3% weight part papoid, 45-50 ℃ of enzyme digestion reaction 10-12h;

D. freezing: by the solution after enzymolysis, centrifugal 30 minutes, rotating speed 4000r/min, collecting precipitation ,-10 ℃ of freezing 24h;

E. melt, concentrate: the frozen material that upper step is obtained takes out, and heating makes it to melt, and filters, and collection filtrate, be condensed into by filtrate the medicinal extract that relative density is 1.22 (60 ℃);

F. medicinal extract is carried out to spraying drying, after packing, get product.

Adopt the bioactive peptide that method obtains that obtains of the present invention, have following proterties:

White crystalline powder; Mildly bitter flavor, soluble in water.

Adopt method of the present invention take Qiezhou stone small water turtle as raw material, to extract bioactive peptide, compared with prior art, advantage of the present invention:

1, stone small water turtle in Qiezhou is hydrolyzed under strong acidic condition, then under the pH of acidity condition, carries out enzymolysis, then, in conjunction with freezing and thawing technique, compares the technology of the activation of existing seed liquor shaking table, inoculation, fermented liquid cultivation, broken wall, and the time shortens greatly;

2, adopt method of the present invention, purification rate and resolution are higher, if do not add acid proteolytic enzyme, do not carry out enzyme digestion reaction, extraction yield is only 2% left and right, and extraction yield is quite low, after adding aspartic protease to carry out enzymolysis, the extraction yield of bioactive peptide is more than 10%, and extraction yield has increased more than 5 times.

Embodiment

Below the invention will be further described with embodiment, but the present invention is not limited to these embodiment.

Embodiment 1:

A kind of is the method that raw material extracts bioactive peptide from Qiezhou stone small water turtle, comprises the steps:

A. raw material is processed: get Qiezhou stone small water turtle, after gilling, clean up, keep turtle shell, tortoise meat;

B. hydrolysis: add the pure water of 8 times of amounts, regulate mixture pH value to 2.5 with 1M HCl, remain pressure 0.1Mpa hydrolysis 10 hours after boiling, boil to remain in press process and note supplementing the moisture that loses;

C. enzymolysis: after hydrolysis completes, filter, collect filtrate, add 1M NaOH adjust pH to 5.5, add 0.3% weight part papoid, 50 ℃ of enzyme digestion reaction 12h;

D. freezing: by the solution after enzymolysis, centrifugal 30 minutes, rotating speed 4000r/min, collecting precipitation ,-10 ℃ of freezing 24h;

E. melt, concentrate: the frozen material that upper step is obtained takes out, and heating makes it to melt, and filters, and collection filtrate, be condensed into by filtrate the medicinal extract that relative density is 1.22 (60 ℃);

F. medicinal extract is carried out to spraying drying, after packing, get product, the extraction yield of bioactive peptide is 10.5%.

Embodiment 2:

A kind of is the method that raw material extracts bioactive peptide from Qiezhou stone small water turtle, comprises the steps:

A. raw material is processed: get Qiezhou stone small water turtle, after gilling, clean up, keep turtle shell, tortoise meat;

B. hydrolysis: add the pure water of 10 times of amounts, regulate mixture pH value to 1.8 with 1M HCl, remain pressure 0.1Mpa hydrolysis 8 hours after boiling, boil to remain in press process and note supplementing the moisture that loses;

C. enzymolysis: after hydrolysis completes, filter, collect filtrate, add 1M NaOH adjust pH to 3.5, add 0.2% weight part papoid, 45 ℃ of enzyme digestion reaction 10h;

D. freezing: by the solution after enzymolysis, centrifugal 30 minutes, rotating speed 4000r/min, collecting precipitation ,-10 ℃ of freezing 24h;

E. melt, concentrate: the frozen material that upper step is obtained takes out, and heating makes it to melt, and filters, and collection filtrate, be condensed into by filtrate the medicinal extract that relative density is 1.22 (60 ℃);

F. medicinal extract is carried out to spraying drying, after packing, get product, the extraction yield of bioactive peptide is 11.2%.

Embodiment 3:

A kind of is the method that raw material extracts bioactive peptide from Qiezhou stone small water turtle, comprises the steps:

A. raw material is processed: get Qiezhou stone small water turtle, after gilling, clean up, keep turtle shell, tortoise meat;

B. hydrolysis: add the pure water of 10 times of amounts, regulate mixture pH value to 2.0 with 1M HCl, remain pressure 0.1Mpa hydrolysis 10 hours after boiling, boil to remain in press process and note supplementing the moisture that loses;

C. enzymolysis: after hydrolysis completes, filter, collect filtrate, add 1M NaOH adjust pH to 4.5, add 0.25% weight part papoid, 50 ℃ of enzyme digestion reaction 12h;

D. freezing: by the solution after enzymolysis, centrifugal 30 minutes, rotating speed 4000r/min, collecting precipitation ,-10 ℃ of freezing 24h;

E. melt, concentrate: the frozen material that upper step is obtained takes out, and heating makes it to melt, and filters, and collection filtrate, be condensed into by filtrate the medicinal extract that relative density is 1.22 (60 ℃);

F. medicinal extract is carried out to spraying drying, after packing, get product, the extraction yield of bioactive peptide is 11.5%.

Claims (1)

1. the method from extraction bioactive peptide the stone small water turtle of Qiezhou, comprise the steps:

A. raw material is processed: get Qiezhou stone small water turtle, after gilling, clean up, keep turtle shell, tortoise meat;

B. hydrolysis: the pure water that adds 8-10 doubly to measure, with 1M HCl, regulate mixture pH value to 1.8-2.5, after boiling, remain pressure 0.1Mpa hydrolysis 8-10 hour, boil to remain in press process and note supplementing the moisture that loses;

C. enzymolysis: after hydrolysis completes, filter, collect filtrate, add 1M NaOH adjust pH to 3.5-5.5, add 0.2-0.3% weight part papoid, 45-50 ℃ of enzyme digestion reaction 10-12h;

D. freezing: by the solution after enzymolysis, centrifugal 30 minutes, rotating speed 4000r/min, collecting precipitation ,-10 ℃ of freezing 24h;

E. melt, concentrate: the frozen material that upper step is obtained takes out, and heating makes it to melt, and filters, and collection filtrate, be condensed into by filtrate the medicinal extract that relative density is 1.22 (60 ℃);

F. medicinal extract is carried out to spraying drying, after packing, get product.