CN105132503B - Stone tortoise active peptide method for refining and prevention cirrhosis, it is antifatigue in application - Google Patents
Stone tortoise active peptide method for refining and prevention cirrhosis, it is antifatigue in application Download PDFInfo
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Abstract
The invention discloses a kind of stone tortoise active peptide method for refining, first use acetic acid dipping pretreatment, soften expanding tissue, remove part foreign protein, enzymatic isolation method is used again and citric acid is added as enzymatic hydrolysis medium, active peptides extraction rate is 25% or more, and prior art recovery rate is 10% or so, and overcome traditional concocted method time consumption and energy consumption, general acid-hydrolysis method amino acid is damaged, the disadvantages of racemization, easily occurs for alkali hydrolysis method, additionally combine multistage membrane separation technique, selectivity is high, by-product is few, it is adaptable, the active peptide purity isolated is higher than 75%, molecular weight is less than 10KD, body circulatory system can be entered with complete form, easily absorb, and it is commonly centrifuged, sedimentation filtration method molecular weight distribution is inhomogenous, assimilation effect is poor.The present invention has been completed at the same time desalination in the process by enzymatic hydrolysis, micro-filtration and multi-ultrafiltration, takes off hardship, obtained stone tortoise active peptide lighter, free from extraneous odour, molecular weight tends to be uniform, safe and non-toxic, in prevention cirrhosis, antifatigue, the healthcare applications such as strengthen immunity have a extensive future.
Description
Technical field
The present invention relates to biological health food technical field, specifically stone tortoise active peptide method for refining and hard in prevention liver
Application in changing, being antifatigue.
Background technique
Today's society, rhythm of life is fast, dog-eat-dog, and work, studying and living pressure are big, and many people are busy with work, thoroughly
Zhi Jiankang, feed is irregular, and diet is uncontrolled, causes the disorder of liver, kidney system, directly or indirectly damages liver kidney, immunity drop
It is low, disease resistance decline, in China, the people there are about 15% be it is healthy, 15% people is in non-health, and 70% people is in " sub-
Health " state.The scale and severity of " inferior health " crowd has allowed of no optimist at present, research and develop protection hepatic and renal tissue,
Prevent cirrhosis, antifatigue, strengthen immunity, it has been very urgent for improving the health care product of people's lives quality and physical fitness
It cuts.
Stone tortoise, i.e. Guangxi intend terrapin, also known as stone small water turtle, Vietnam's stone tortoise, Guangxi terrapin, pond turtle with yellow throat, as medicinal head
Be loaded in Han dynasty Shennong's Herbal, be classified as top grade, hereafter how on the books book on Chinese herbal medicine is, wherein " book on Chinese herbal medicine is looked for the truth " speech " specially enter kidney, it is simultaneous enter
The heart, Gan Xian are slightly cold ", Compendium of Material Medica records road, and " (tortoise) can lead to conception vessel, therefore take its first with bushing, kidney tonifying, remove hemostasis, hemostasis
Dysentery, continuous muscles and bones control overstrain, subduing inflammation ".Since ancient times, the health-care efficacy that China just has food tortoise to keep fit and healthy, modern medicine study
Show tortoise essential amino acid rich in, protein, cutin, ossein, Ca, P, K and multivitamin, blood glucose, rouge
The nutritional ingredients such as fat acid, human body easily absorb, in addition resistance to hungry, the resistance to life habit thirsty, cold-resistant, the service life is long of tortoise, constitutes its body
A variety of long-lived cell factors have nourishing and suppressing Yang, and the strong bone of kidney-nourishing, consolidating channel for hemostasis, heart tonifying and tranquilizing can be used for the deficiency of the kidney yin, the deficiency of Yin
Partially high, night sweat, spermatorrhea etc. is antifatigue etc. to show preferable health-care efficacy in particular for liver fibrosis, and also studies have found that
Stone tortoise active peptide can promote cancer patient's internal Leukocyte Growth after chemicotherapy, restores health, reaches anti-cancer efficacy.
Qiezhou, is located in Subtropical Coastal area, benign climate, since geographical natural condition is richly endowed by nature, Qiezhou stone small water turtle
Closer to natural ecology product, excellent quality, it has also become Chinese geography famous special product, with its exclusive nutrition and health care, economic valence
Value becomes the up-and-coming youngster of famous-brand and high-quality cultivation, and stone tortoise raiser is already expired ten thousand at present, and annual value of production is more than 3.5 hundred million, however, although the source of goods fills
Foot, but single cultivation sales mode is also more stayed in, the product category listed is less such as: fresh and alive quick frozen product, canned food,
And often technique is coarse, poor taste, lacks the market competitiveness, intensive processing is still in space state.How to be extracted from stone tortoise,
The a big difficulty that active peptide is still research is isolated and purified out, traditional concocted method effective component is fried to need the long period, and energy consumption is high,
General acid extraction method partial amino-acid is damaged, and product yield is low, buck extraction method extract containing hydroxyl and sulfydryl
Racemization easily occurs for amino acid, peptides inactivation, and isolating and purifying for peptides is a systematic engineering of business, general sedimentation filtration,
Centrifuge separation, solvent extraction are to be extremely difficult to good separation effect, therefore the present invention develops one on the basis of many experiments early period
Kind of stone tortoise deep working method, recovery rate is high, isolates and purifies that effect is good, and activity is high, it is intended to Qiezhou local supplies advantage is made full use of,
System research is goed deep into stone tortoise nutrition, health value, the integral framework that formation scientific research, production, deep processing, sale are integrated is ground
The stone tortoise active peptide produced has preferable prevention cirrhosis, antifatigue, strengthen immunity health-care efficacy.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide it is a kind of it is simple and safe, easy to operate, product purity is high
Stone tortoise active peptide method for refining, additionally provide its prevention cirrhosis, anti-fatigue health-caring application, it is intended to make full use of ground ashlar
Tortoise resource develops the deep processing health care product more having a extensive future.
To achieve the above object, the present invention provides stone tortoise active peptide method for refining, include the following steps:
1) pretreatment of raw material: stone tortoise is put to death, decaptitating, reject internal organ, wash 3-4 time, crush, tune pH 2-3,10 DEG C with
Lower immersion for 24 hours, takes out after expanding uniformly, cleans 2-3 times repeatedly with water to no tart flavour, drain, add 8-10 times of water to be beaten, until even
Slurries add water to supply weightlessness；
2) it digests: 1% (w/w) citric acid being added into the homogenate that step 1) obtains as enzymatic isolation method Extraction medium, adjusts
PH 2-4 is saved, the pepsin of 0.5-2% (w/w) is added, digests 5-7h at 4 DEG C, stirs 10-15min per hour, heats, goes out
Enzyme activity obtains stone tortoise enzymolysis liquid；
3) multistage membrane separating and purifying:
1. micro-filtration: the stone tortoise enzymolysis liquid for taking step 2) to obtain, being diluted with water to mass fraction is 10%, micro- with 0.3 μm of film
Filter, operating pressure 0.2MPa；
2. level-one ultrafiltration: 1. filtrate that step is obtained is by the ultrafiltration of 20kD ultrafiltration membrane, operating pressure 0.2-0.25MPa,
Ultrafiltration 2-3 times；
3. two-stage ultrafiltering: by step, 2. gained filtrate uses ultrafiltration 2 times under 0.8MPa operating pressure of 10kD ultrafiltration membrane again,
Permeate is obtained, drying is concentrated under reduced pressure in high pressure sterilization；
4. regular Membrane cleaning: after reaction a period of time, ultrafiltration membrane has gel layer, and membrane flux can reduce, and carries out Membrane cleaning, first
It is rinsed with water about 15min, until permeate colorless and odorless, adds 0.5% (w/w) sodium hydroxide repeated flushing 15min, be washed to filtration
Liquid is neutrality；
4) it sterilizes, is dry, obtaining stone tortoise active peptide powder.
The preferred pure water of water used in the present invention.
The preferred 1:20 of the additive amount of acetic acid described in step 1) (w/v).
Stirring described in step 2), it is preferred to use magnetic stirrer stirring.
Sterilizing described in step 4), drying, it is preferred to use spray drying, 120-150 DEG C of inlet air temperature, leaving air temp 70-
90℃。
The invention further relates to the stone tortoise active peptide obtained using above-mentioned method for refining and its in prevention cirrhosis, antifatigue
In application, can also add pure water according to actual needs, appropriate essence, using existing preparation technique and process, with
Oral preparation, including oral solution, granule, tablet, capsule etc. is made in common auxiliary material.
Compared to the prior art, the present invention has the advantages that
1, in the prior art, general acid extraction method partial amino-acid is damaged, and product yield is low, and buck extraction method is extracted
The disadvantages of racemization, easily occurs for the amino acid containing hydroxyl and sulfydryl out.The present invention is added 1% citric acid and mentions as enzymatic hydrolysis
Medium is taken, PH2-4 is adjusted, acid reaction environment is provided, raw material is swollen, structure is more fluffy, and it is conducive to pepsin and permeates, and
It is that pepsin is in optimum pH in such circumstances, enzymatic activity improves, and active peptides extraction rate overcomes biography 25% or more
The shortcomings that system concocted method is easily precipitated in extraction process, time consumption and energy consumption.
2, the present invention considers stone tortoise enzymolysis liquid and contains a large amount of protein matter, operation ultrafiltration membrane table after a certain period of time
Face can adsorb a certain amount of protein substance, form gel layer, make flux depression, influence separating effect, disclose a kind of behaviour
Make simplicity, quickly, feasible film method for cleaning washes Membrane cleaning using 0.5% sodium hydroxide circulation after reacting a period of time,
When it is neutral for measuring filtered solution, this step can guarantee that system maintains a penetrating amount of ideal film.
3, the present invention before carrying out multi-ultrafiltration by 0.3 μm microfiltration membranes pretreatment, prevent macromolecular suspended impurity and
Protein contamination ultrafiltration membrane, then big molecular impurity albumen is removed by the ultrafiltration of 20kD ultrafiltration membrane, filtrate is obtained using 10kD ultrafiltration
Film removes some inorganic salts and small molecular weight impurity, finally obtains stone tortoise active peptide purity 75% or more, and molecular weight is less than 10KD,
Body circulatory system can be entered with complete form, it is close with normal person's body physiological product, be conducive to body and absorb, and it is general from
The heart, sedimentation filtration method molecular weight distribution are inhomogenous, and hardly possible absorbs, and have been completed at the same time desalination in this micro-filtration, multistage membrane separating process, have taken off
Hardship, obtained stone tortoise active peptide color is thin out, in good taste.
4, the present invention realizes effective control of enzymolysis product molecular weight distribution using micro-filtration and multistage UF membrane enzymolysis liquid
System, molecular weight are less than 10KD, and ingredient tends to be uniform, and property is stablized, and carry out medicine with enzymolysis liquid obtained by common centrifugal separation
Effect, which is learned, to be compared, as a result, it has been found that for CCl4The liver of caused Liver Damage in Rats model, common centrifugal separation group rat shows yellowish
Color has slight swelling, shows in particle nodositas, and multistage UF membrane group, rat liver between bronzing and it is faint yellow it
Between, apparent particle tubercle object is not found, this is because oligopeptides molecular weight distribution is wide in the resulting active peptide of common separation, is not easy
It absorbs, and oligopeptides molecular weight is concentrated in Multistage Membranes isolating active peptide, small-molecular peptides content is high, and activity is strong, makes liver fibrosis
It reverses, in protection hepatic tissue, prevention cirrhosis effect is become apparent from.
Specific embodiment
Below with examples illustrate the present invention, but the invention is not limited to these embodiments.
Embodiment 1
Stone tortoise active peptide method for refining, includes the following steps:
1) pretreatment of raw material: stone tortoise is put to death, decaptitating, rejects internal organ, and clear water washs 3 times, is just crushed, is added PH 2.5 or so
Acetic acid (1:20W/V), 5 DEG C impregnate for 24 hours, wait expand uniformly after take out, add water to clean 2 times repeatedly to no tart flavour, drain, add water
8 times of mashing, until homogenate, adds water to supply weightlessness, it is spare；
2) it digests: 1% citric acid being added in homogenate as enzymatic isolation method Extraction medium and adjusts PH to 3,1% stomach is added
Protease digests 5h at 4 DEG C, and every 1h stirs 15min using magnetic stirrer, and heating, enzyme deactivation is living, obtains stone tortoise enzymolysis liquid；
3) multistage membrane separating and purifying:
1. micro-filtration: taking stone tortoise enzymolysis liquid, being diluted with water to mass fraction is about 10%, with 0.3 μm of membrane microfiltration, operating pressure
For 0.2MPa；When the 80% of micro-filtration to additional amount, suitable quantity of water is added into trapped fluid, stirs, continues micro-filtration, is received to micro-filtrate
When collection to volume is equal to medical fluid additional amount, stops micro-filtration, it is spare to obtain micro-filtrate；
2. level-one ultrafiltration: filtrate is by the ultrafiltration of 20kD ultrafiltration membrane, operating pressure 0.2MPa, ultrafiltration 2 times；
3. two-stage ultrafiltering: filtrate obtained by level-one ultrafiltration uses ultrafiltration 2 times under 0.8MPa operating pressure of 10kD ultrafiltration membrane again,
Obtain permeate；
4. regular Membrane cleaning: after reaction a period of time, if discovery ultrafiltration membrane has gel layer to need to carry out when membrane flux reduces
Membrane cleaning is first rinsed with water about 15min, until permeate colorless and odorless is washed to 0.5% sodium hydroxide repeated flushing 15min
Filtered solution is neutrality；
4) it sterilizes, dry: sterilizing, it is dry using spray drying process, 120 DEG C of inlet air temperature, 90 DEG C of leaving air temp, obtain stone tortoise
Active peptide powder.
Embodiment 2
Stone tortoise active peptide method for refining, includes the following steps:
1) pretreatment of raw material: stone tortoise is put to death, decaptitating, rejects internal organ, and clear water washs 4 times, is just crushed, is added PH 2.5 or so
Acetic acid (1:20W/V), 10 DEG C impregnate for 24 hours, wait expand uniformly after take out, add water to clean 3 times repeatedly to no tart flavour, drain, add
9 times of water mashing, until homogenate, adds water to supply weightlessness, it is spare；
2) it digests: 1% citric acid being added in homogenate as enzymatic isolation method Extraction medium and adjusts PH to 3,2% stomach is added
Protease digests 7h at 4 DEG C, and every 1h stirs 15min using magnetic stirrer, and heating, enzyme deactivation is living, obtains stone tortoise enzymolysis liquid；
3) multistage membrane separating and purifying:
1. micro-filtration: taking stone tortoise enzymolysis liquid, being diluted with water to mass fraction is about 10%, with 0.3 μm of membrane microfiltration, operating pressure
For 0.2MPa；When the 80% of micro-filtration to additional amount, suitable quantity of water is added into trapped fluid, stirs, continues micro-filtration, is received to micro-filtrate
When collection to volume is equal to medical fluid additional amount, stops micro-filtration, it is spare to obtain micro-filtrate；
2. level-one ultrafiltration: filtrate is by the ultrafiltration of 20kD ultrafiltration membrane, operating pressure 0.25MPa, ultrafiltration 3 times；
3. two-stage ultrafiltering: filtrate obtained by level-one ultrafiltration uses ultrafiltration 2 times under 0.8MPa operating pressure of 10kD ultrafiltration membrane again,
Obtain permeate；
Regular Membrane cleaning: after reaction a period of time, if discovery ultrafiltration membrane has gel layer that need to carry out film when membrane flux reduces
Cleaning, is first rinsed with water about 15min, until permeate colorless and odorless is washed to filter with 0.5% sodium hydroxide repeated flushing 15min
Liquid is crossed as neutrality；
4) it sterilizes, dry: sterilizing, it is dry using spray-on process, 150 DEG C of inlet air temperature, 80 DEG C of leaving air temp, obtain stone tortoise activity
Peptide powder.
Embodiment 3
Stone tortoise active peptide method for refining, includes the following steps:
1) pretreatment of raw material: stone tortoise is put to death, decaptitating, rejects internal organ, and clear water washs 3 times, is just crushed, is added PH 2.5 or so
Acetic acid (1:20W/V), 10 DEG C impregnate for 24 hours, wait expand uniformly after take out, add water to clean 3 times repeatedly to no tart flavour, drain, add
10 times of water mashing, until homogenate, adds water to supply weightlessness, it is spare；
2) it digests: 1% citric acid being added in homogenate as enzymatic isolation method Extraction medium and adjusts PH to 3,2% stomach is added
Protease digests 6h at 4 DEG C, and every 1h stirs 15min using magnetic stirrer, and heating, enzyme deactivation is living, obtains stone tortoise enzymolysis liquid；
3) multistage membrane separating and purifying:
1. micro-filtration: taking stone tortoise enzymolysis liquid, being diluted with water to mass fraction is about 10%, with 0.3 μm of membrane microfiltration, operating pressure
For 0.2MPa；When the 80% of micro-filtration to additional amount, suitable quantity of water is added into trapped fluid, stirs, continues micro-filtration, is received to micro-filtrate
When collection to volume is equal to medical fluid additional amount, stops micro-filtration, it is spare to obtain micro-filtrate；
2. level-one ultrafiltration: filtrate is by the ultrafiltration of 20kD ultrafiltration membrane, operating pressure 0.2MPa, ultrafiltration 3 times；
3. two-stage ultrafiltering: filtrate obtained by level-one ultrafiltration uses ultrafiltration 2 times under 0.8MPa operating pressure of 10kD ultrafiltration membrane again,
Obtain permeate；
4. regular Membrane cleaning: after reaction a period of time, if discovery ultrafiltration membrane has gel layer to need to carry out when membrane flux reduces
Membrane cleaning is first rinsed with water about 15min, until permeate colorless and odorless, adds 0.5% sodium hydroxide repeated flushing 15min, be washed to
Filtered solution is neutrality；
4) it sterilizes, dry: sterilizing, it is dry using spray-on process, 150 DEG C of inlet air temperature, 70 DEG C of leaving air temp, obtain stone tortoise activity
Peptide powder.
As a result:
Experimental example: pharmacodynamic experiment
Test 1 stone tortoise active peptide effect of anti hepatic fibrosis
1, test solution
Stone tortoise active peptide powder made of embodiment 3, adds water ultrasonic dissolution, and is configured to every milliliter contained 2g.
2, experimental animal
SD rat, 60, male, SPF grades, weight 160-200g.
3, method
SD rat is randomly divided into 5 groups, respectively blank control group, model group, positive controls, stone after adapting to environment 5 days
Tortoise active peptide high dose group, stone tortoise active peptide low dose group, every group 10, model group and each test administration group rat back are subcutaneous
Inject 40%CCl4, 2 times a week, modeling for the first time is subcutaneously injected by 5ml/kg, is subcutaneously injected later by 3ml/kg, is amounted to 12 weeks.
Normal group subcutaneous injection same amount of normal saline.Continuous 12 weeks backward, normal group and model group gave stomach-filling physiological saline, positive right
Colchicin 0.2mg/kg/d is given according to group, stone tortoise active peptide high and low dose group gives test test sample medication amount difference respectively
Are as follows: 12mg/kg/d, 4mg/kg/d.After experiment, all Rat Fasts can't help water 12h, 10% chloral hydrate anesthesia, broken end
Blood is taken, serum is separated, radioimmunology surveys III, IV-C of HA, LN, PC.
4, data processing
Experimental data indicates that data statistics is carried out with SPSS11.0 software with (X ± s), and control uses one way between group
Anova is carried out.
Influence of each test group to hepatic fibrosis index
Note: ## P < 0.01* compared with blank group, * * P < 0.05 compared with model control group, P < 0.01
5, analysis and discussion
By upper experimental result it is found that model group III, IV equal conspicuousness of-C of HA, LN, PC in rat blood serum compared with normal group
It increases (P < 0.01), shows modeling success.Positive controls and each dosage group of stone tortoise active peptide compared with model group, HA, LN,
The equal conspicuousness of PC III, IV-C reduces (P < 0.05), has statistical significance.Some researches show that liver fibrosis is a variety of Chronic Livers
Disease develops into the pathological basis of cirrhosis, lives for a long time irregular, and the stimulation liver such as drink can lead to liver fibrosis, tests
Show that stone tortoise active peptide can reduce degree of hepatic fibrosis, reverse liver fibrosis, there is protection liver, prevent the health care function of cirrhosis
Effect.
Test the 2 antifatigue researchs of stone tortoise active peptide
1, test drug
Stone tortoise active peptide powder made of embodiment 3, adds water ultrasonic dissolution, and is configured to every milliliter contained 0.5g.
2, animal
Kunming mouse, male, SPF grades, weight (20 ± 2) g
3, method
Kunming mouse is taken to be divided into 4 groups, every group 10, respectively blank group, the high, medium and low dosage group of stone tortoise active peptide.Stone
It is respectively 50mg/kg/d, 25mg/kg/d, 10mg/ that the high, medium and low dosage group of tortoise active peptide gives test test sample medication amount respectively
Kg/d, blank group stomach-filling respective volume physiological saline.
Loaned swimming test: after last dose 30min, mouse is set on water tank went swimming, depth of water 30cm, 25 DEG C of left sides of water temperature
The right side, the plasticine of 5% weight of rat-tail root load.Record mouse from swim to death time, as swimming time.
Serum urea nitrogen determination: after last dose 30min, the eye after temperature 25 DEG C of water went swimming 90min, rest 60min
Socket of the eye takes blood, and colorimetric method surveys serum urea nitrogen.
4, result
The influence of stone tortoise Active Peptide in Mice swimming time and serum urea nitrogen
Note: *, * * P < 0.05, P < 0.01 compared with blank group control group
5, analysis and discussion
According to the experimental results, the high, medium and low dosage group mouse swimming time of stone tortoise active peptide is longer compared with the blank group time,
The strenuous exercise for prompting stone tortoise active peptide that body is made to be resistant to the longer time, the generation of delay fatigue or the elimination to accelerate fatigue.
Serum urea nitrogen is the sensitive indexes for evaluating body load ability to bear.Body serum urea nitrogen content is with labour and exercise load
Increase and increase, body is poorer to load performance, and serum urea nitrogen increase is more obvious, serum urea nitrogen each group when tranquillization
Between difference it is little, after 90min swimming, stone tortoise active peptide is high, middle dose group mice serum urea nitrogen is lower than control group (P < 0.05),
It is statistically significant, it prompts stone tortoise active peptide that body can be improved to the adaptability of load, there is preferable anti-fatigue health efficacy.
Claims (5)
1. stone tortoise active peptide method for refining, which comprises the steps of:
1) pretreatment of raw material: stone tortoise is put to death, decaptitating, rejects internal organ, is washed 3-4 time, crushing, with acetic acid tune pH 2-3,10 DEG C
It impregnates for 24 hours, is taken out after expanding uniformly below, clean 2-3 times repeatedly with water to no tart flavour, drain, add 8-10 times of water to be beaten, until
Homogenate adds water to supply weightlessness；
2) it digests: 1% citric acid being added into the homogenate that step 1) obtains as enzymatic isolation method Extraction medium, adjusting pH is 3, is added
Enter 2% pepsin, 6h is digested at 4 DEG C, every 1h stirs 15min using magnetic stirrer, and heating, enzyme deactivation is living, obtains stone tortoise enzyme
Solve liquid；
3) multistage membrane separating and purifying:
1. micro-filtration: the stone tortoise enzymolysis liquid for taking step 2) to obtain, being diluted with water to mass fraction is 10%, with 0.3 μm of membrane microfiltration, behaviour
Making pressure is 0.2MPa；
2. level-one ultrafiltration: 1. filtrate that step is obtained passes through the ultrafiltration of 20kD ultrafiltration membrane, operating pressure 0.2-0.25MPa, ultrafiltration
2-3 times；
3. two-stage ultrafiltering: by step, 2. gained filtrate uses ultrafiltration 2 times under 0.8MPa operating pressure of 10kD ultrafiltration membrane again, obtains
Drying is concentrated under reduced pressure in permeate, high pressure sterilization；
4. regular Membrane cleaning: after reaction a period of time, ultrafiltration membrane has gel layer, and membrane flux can reduce, and carries out Membrane cleaning, first uses water
15min is rinsed, until permeate colorless and odorless, adds 0.5% sodium hydroxide repeated flushing 15min, is washed to filtered solution as neutrality；
4) it sterilizes, is dry, obtaining stone tortoise active peptide powder.
2. stone tortoise active peptide method for refining according to claim 1, it is characterised in that: the additive amount of acetic acid described in step 1)
For 1:20.
3. stone tortoise active peptide method for refining according to claim 1, it is characterised in that: stirring described in step 2), using magnetic
The stirring of power blender.
4. the stone tortoise active peptide that any stone tortoise active peptide method for refining obtains in claim 1-3.
5. application of the stone tortoise active peptide as claimed in claim 4 in preparation prevention cirrhosis, anti-fatigue medicament.
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| CN112941137A (en) * | 2021-03-25 | 2021-06-11 | 海南金龟岛生物科技有限公司 | Production method of tortoise peptide |
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| US6372261B1 (en) * | 2000-07-07 | 2002-04-16 | Pharmaceutical Industry Technology And Development Center | Process for producing Guilu ErXiangao |
| CN103397066A (en) * | 2013-07-29 | 2013-11-20 | 广西还珠海洋生物科技有限公司 | Method for extracting active peptides from Qinzhou yellow pond turtle |
| CN104630318A (en) * | 2015-01-21 | 2015-05-20 | 华南理工大学 | Preparation method of small water turtle anti-tumor polypeptide |
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2015
- 2015-09-22 CN CN201510606682.5A patent/CN105132503B/en active Active
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| US6372261B1 (en) * | 2000-07-07 | 2002-04-16 | Pharmaceutical Industry Technology And Development Center | Process for producing Guilu ErXiangao |
| CN103397066A (en) * | 2013-07-29 | 2013-11-20 | 广西还珠海洋生物科技有限公司 | Method for extracting active peptides from Qinzhou yellow pond turtle |
| CN104630318A (en) * | 2015-01-21 | 2015-05-20 | 华南理工大学 | Preparation method of small water turtle anti-tumor polypeptide |
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