CN103355360A - Green-grass traditional Chinese medicine aerosol disinfectant - Google Patents
Green-grass traditional Chinese medicine aerosol disinfectant Download PDFInfo
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- CN103355360A CN103355360A CN2013101303083A CN201310130308A CN103355360A CN 103355360 A CN103355360 A CN 103355360A CN 2013101303083 A CN2013101303083 A CN 2013101303083A CN 201310130308 A CN201310130308 A CN 201310130308A CN 103355360 A CN103355360 A CN 103355360A
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Abstract
The invention relates to the technical field of medical treatment and hygiene, particularly relates to a disinfectant and discloses a green-grass traditional Chinese medicine aerosol disinfectant. The green-grass traditional Chinese medicine aerosol disinfectant is prepared from Chinese medicinal herbal raw materials, wherein the Chinese medicinal herbal raw materials comprise the following ingredients in percentage by weight: 5-15% of barberry root, 5-15% of phellodendron bark, 0-10% of gentian, 5-15% of ash bark, 5-15% of peony, 5-15% of glabrous sarcandra herb, 5-15% of thalictrum root, 10-20% of mahonia stem, 5-15% of Chinese mahonia leaf and 5-15% of honeysuckle flower. Due to the adoption of the technical scheme, the green-grass traditional Chinese medicine aerosol disinfectant has a remarkable sterilization effect on staphylococcus aureus and candida albicans, and is short in sterilization time and good in sterilization effect.
Description
Technical field
The present invention relates to the medical sanitary technology field, relate in particular to a kind of disinfectant.
Background technology
Disinfectant is used for killing pathogenic microorganism on the communication media, makes it reach innoxious requirement, and pathogenic microorganism is eliminated outside human body, cuts off the route of transmission of infectious disease, reaches the purpose of control infectious disease.Disinfectant of the prior art, general adopt " chemosterilant " adopts the chemical compound killing microorganisms to make it reach the sterilization requirement." chemosterilant " to human body have spread effect, taste is unpleasant, corrosivity is more intense, easily causes danger.People also adopt the mode of ultraviolet sterilization, and this mode need to operate in unmanned situation.Although and ultraviolet germicidal effect is stronger, very weak to the penetration capacity of object.It is applicable to thermo-labile article and mesa surfaces sterilization, and ultraviolet sterilization is to the air sterilization effect extreme difference.
Therefore people study and adopt Chinese herbal medicine or extracted form natural plant disinfectant to carry out the sterilization sterilization.Be in 200410067078.1 the patent, to adopt the solvent of caryophyllus oil and water, ethanol or their compositions of mixtures to make disinfectant such as the patent No., this disinfectant is free from side effects to human body, has good sterilization, bactericidal effect.In the background technology of this patent, also mentioned in the prior art with the example of natural plants as the fungicidal activity component.Make a general survey of Chinese herbal disinfectant of the prior art, compare with chemosterilant with ultraviolet sterilization, all have the problems such as the sterilization sterilization time is long, bactericidal effect is not remarkable.
Summary of the invention
The object of the invention is to, provide a kind of greenweed medicinal herbs aerosol inhalation disinfectant, to solve the problems of the technologies described above.
Technical problem solved by the invention can realize by the following technical solutions:
Greenweed medicinal herbs aerosol inhalation disinfectant, adopt herbal raw material to make disinfectant, it is characterized in that, described herbal raw material comprises following component: barberry 5%-15%, golden cypress 5%-15%, felwort 0%-10%, bark of ash 5%-15%, tree peony 5%-15%, Glabrous Sarcandra Herb 5%-15%, manyleaf meadowure rhizome and root 5%-15%, leatherleaf mahonia 10%-20%, Chinese mahonia leaf 5%-15%, honeysuckle 5%-15%.
The present invention adopts said herbal medicine to adopt the mode of preserved material to make disinfectant, has realized the plant extraction liquid of pure natural, and edible is free from side effects and toxicity fully to human body.
Preferred described herbal raw material comprises following component: barberry 10%, golden cypress 10%, felwort 5%, the bark of ash 10%, tree peony 10%, Glabrous Sarcandra Herb 10%, manyleaf meadowure rhizome and root 10%, leatherleaf mahonia 15%, Chinese mahonia leaf 10%, honeysuckle 10%.
Comprise jamaicin 70% in the disinfectant that described herbal raw material is made.In order to reach preferably bactericidal effect, especially staphylococcus aureus and Candida albicans had significant bactericidal action.
Also comprise essential oil 2% in the disinfectant that described herbal raw material is made.So that smell fragrance of the present invention, after the use, not only antibiotic further stimulation brain makes human body present the most comfortable state.
Disinfectant of the present invention is compared with ultraviolet sterilization, and Disinfection Effect is better than disinfection efficacy of ultraviolet ray.Disinfectant sterilization of the present invention is even, and disinfection by ultraviolet light is inhomogeneous, and disinfectant of the present invention is with low cost, easy to use, and the disinfection by ultraviolet light cost is high, uses inconvenience, and especially fluorescent tube is perishable.
Disinfectant of the present invention is compared with existing " chemosterilant ", adopts 100% natural botanical extraction liquid, and is nontoxic, harmless, have no side effect, non-stimulated to human body.
The present invention can carry out degerming in conjunction with the aerosol sterilization device in use, also can adopt the electric-heating fumigator of mosqito that generally uses on the market to use.The present invention can also adopt hand-driven pressure spraying degerming, can also adopt supporting air cleaner degerming.
Beneficial effect: owing to adopting technique scheme, the present invention has significant bactericidal action to staphylococcus aureus and Candida albicans, and sterilizing time is short, bactericidal effect is good.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below further set forth the present invention.
Greenweed medicinal herbs aerosol inhalation disinfectant, adopt herbal raw material to make disinfectant, herbal raw material comprises following component: barberry 5%-15%, golden cypress 5%-15%, felwort 0%-10%, bark of ash 5%-15%, tree peony 5%-15%, Glabrous Sarcandra Herb 5%-15%, manyleaf meadowure rhizome and root 5%-15%, leatherleaf mahonia 10%-20%, Chinese mahonia leaf 5%-15%, honeysuckle 5%-15%.Preferred herbal raw material comprises following component: barberry 10%, golden cypress 10%, felwort 5%, the bark of ash 10%, tree peony 10%, Glabrous Sarcandra Herb 10%, manyleaf meadowure rhizome and root 10%, leatherleaf mahonia 15%, Chinese mahonia leaf 10%, honeysuckle 10%.The present invention adopts said herbal medicine to adopt the mode of preserved material to make disinfectant, has realized the plant extraction liquid of pure natural, and edible is free from side effects and toxicity fully to human body.
The licensing that the health that the disinfectant that the present invention makes is also successively issued by National Health Service is examined is defended medicament 92.001[such as the Fengxian and is newly defended], disappear No. the 022nd, careful (nuclear) word (1993), Hu Wei of Hu Wei disappear and demonstrate,prove disappear No. the 019th, card (XD) word of No. the 0216th, word (2003), (2003) Hu Wei.
Disinfectant process Senior Residents in Fengxian District of Shanghai institute for drug control, Second Military Medical University, PLA and the Shanghai City medical industry research institute that the present invention makes and Shanghai City health and epidemic prevention station, Shanghai City Disease Control and Prevention Center are detected, the disinfection wide spectrum, can significantly kill the various malignant bacterias such as staphylococcus aureus, Candida albicans in the air, toxicity detects, the nontoxic level in true border.And infected by influenza, hepatitis viruse, rubella virus and adenovirus, respiratory tract infection virus waits also remarkable killing effect.Nontoxic, have no side effect, meet the hospital operating room air sterillization sanitary standard of national regulation fully.
The disinfectant that the present invention makes is through Shanghai No.1 Pulmonary Department Hospital, the second hospital of lung section, Shanghai City institute of traditional Chinese medicine, Central Hospital of Huangpu Dist., Shanghai, Longhua, Shanghai City hospital, Fengxian, Shanghai City institute of traditional Chinese medicine, Jinshan District central hospital, the woman of Songjiang District protects the institute maternity ﹠ child care center, Songjiang District mental health center, Songjiang District squarer institute of traditional Chinese medicine, kindergarten, Yueyang, Songjiang District, the Gu Yang kindergarten, from the cloud kindergarten, squarer kindergarten, Songjiang District, literary composition Xiang kindergarten, Songjiang District, the various big hospitals such as new bridge kindergarten, Songjiang District and kindergarten use sterilization effect remarkable for a long time, smell delicate fragrance is the disinfectant of the popular epidemic disease of prevention and contagiosity epidemic disease.
Embodiment 1:
The said herbal medicine raw material is made disinfectant, adopt aerosol can in conjunction with disinfectant of the present invention, realize the aerosol disinfective action.Requiring aerosol can to spray should be unblocked, and is vaporific.Adopt the GB15981 regulation to carry out the bacterial inactivation rate evaluation, effect is as follows:
| Project | Index |
| Staphylococcus aureus (stoste effect 15min), % | ≥99.9 |
| Candida albicans (stoste effect 45min), % | 100 |
Conclusion: disinfectant is good to the staphylococcus aureus sterilization effect, and is remarkable to the Candida albicans effect.
Embodiment 2:
With disinfectant of the present invention, adopt red swallow sprayer, the multistage impacting type air sampler of F-1 type, contain the nutrient agar incubator of 0.5% sodium thiosulfate, test.
Select 25m
2An operating room, sampler is put central authorities 1.0m place, chamber, adopt sampler to carry out airborne natural bacteria sampling 1 minute before the sterilization, flow is 28.3 liter/mins of clocks, as the sample (positive control) before the sterilization, then sprayed 10 minutes to room air with sprayer, consume disinfectant liquid 15 grams, sterilize after 45 minutes, as stated above sampling, carry out viable bacteria and cultivate counting, calculate extinction rate.Test repeats 3 times.The result is as follows:
Attached neutralizer test:
Bacterial strain is Escherichia coli (8099), adopts disinfectant of the present invention, and neutralizer adopts the PBS of 0.5% sodium thiosulfate, and conclusion is as follows:
Conclusion: the present invention consumes disinfectant 15g through spraying disinfection 45 minutes, and extinction rate reaches more than 94.67% in the room air, and Total Bacteria Count of Bacteria in Indoor Air meets national permission standard.
Embodiment 3:
The said herbal medicine raw material is made disinfectant, adopt inhalator jar in conjunction with disinfectant of the present invention, the examination air sterilization effect comprises artificial aerosol sterilization experiment and on-site disinfection test binomial content, and the result is as follows:
One, artificial aerosol sterilization experiment:
Material: international standard bacterial strain, staphylococcus aureus ATCC6538;
1M
3Inhalator jar with fan;
Full glass concentric circles reverse-flow type sprayer below droplet size 5 μ, accounts for about 90%.
Hydraulic shock formula sampler.
Method: with the golden Portugal bacterium bacterium liquid of 103cfu/ml concentration to aerosol container spraying bacterium 3 minutes, open simultaneously fan, make the aerosol even spraying complete, after static 2 minutes, adopt device sampling 1 minute with the hydraulic shock formula, flow 10 is upgraded to the front air-borne bacteria content of sterilization, plugs immediately the inhalator jar power supply, timing: 30 minutes, 60 minutes, 90 minutes each once sampling, sample solution are done the viable bacteria counting.Make not have simultaneously the check experiment of sterilization, ask the Natural Decline rate, obtain at last sterilizing rate, disinfectant of the present invention is as follows to airborne staphylococcus aureus killing effect:
Two, on-site disinfection test
The burn ward carries out in test hospital, and this ward central air conditioning is without purifier, and temperature is selected 70 cubic metres of ward volumes at 23-35 ℃, and patient is 3 people.Personnel's turnover has 6-15 person-time at the trial, adopts SS-II type fissure type air sampler to sample, and establishes two sampled points, and one another is put near the doorway in ward central authorities, once sampling before the sterilization, and in contrast, flow 27.5L/ divides.Rear 20 minutes, 40 minutes, the 60 minutes respectively once sampling of sterilizing, culture dish is placed on 37 ℃ of incubators and cultivated 48 hours, counting,
Obtain each cubic metre bacterium number, the result is as follows:
| Processing time | Bacterium number (cfu/m 3） |
| 0 | 1981.78 |
| 20 | 1186.87 |
| 40 | 618.18 |
| 60 | 500.00 |
[0045]Show that from upper table this disinfectant can reach hospital operating room aerial bacterioplankton III grade standard in sterilization after 60 minutes.
Embodiment 4:
Disinfectant of the present invention is detected test result according to " disinfection technology standard " and " test requirement specification ":
1. to the killing effect of staphylococcus aureus in the air: at 1m
3In the aerosol cabinet, spray medicine 3.0 gram acts on 15 minutes, all can reach 99.9% three times to the killing rate of staphylococcus aureus in the air.
2. to the killing effect of Candida albicans in the air: at 1m
3In the aerosol cabinet, spray medicine 4.3 gram acts on 45 minutes, and the killing rate of Candida albicans in the air is 100.00% three times.
3. stability test: disinfectant is placed in 54 ℃ of water-baths and 20 ℃ in greenhouse after 14 days, carries out the air sterillization test.The result shows, the killing rate of two groups of disinfectants is without significant change.
4. simulated field test: at 27m
3In the aerosol chamber, spray medicine 15 gram acts on 45 minutes, can reach 99.9% to the killing rate of airborne staphylococcus aureus.
Embodiment 5:
Report number is detected in the epidemiology teaching and research room of disinfectant warp let-off The 2nd Army Medical College that the present invention makes: 20010528, test as follows:
One, equipment
Strain name and bacterial strain number, the unit of providing, cultivate algebraically.
Staphylococcus aureus ATCC6538 strain, the tenth generation.Beijing preventive medicine academy of sciences provides.
Candida albicans ATCC10231 strain, the 8th generation.Beijing preventive medicine academy of sciences provides.
2. microbiological contamination aerosol generator:
Full glass concentric circles sprayer, the ejection particle diameter is 1-6um more than 90%.
3. test place: 1m
3Air sterillization mist cabinet.
4. disinfectant title and effective ingredient: greenweed medicinal herbs aerosol inhalation disinfectant, Main Ingredients and Appearance are Chinese herbal medicine.
5. neutralizer composition and concentration: 0.5% soft phosphatide+3% Tween-80
6. sampler: full glass precursor solution impact sampler.
Two, method:
1. detection foundation
(1) bacterial culture counting (2.2) and (2) air sterilization effect identification experiment technology (6) in Ministry of Health of the People's Republic of China's " disinfection technology standard "
2. test temperature and relative moisture
Temperature: natural temperature is at 20-24 ℃ during test
Humidity: dehumidifier and humidification apparatus are arranged, and humidity is controlled at about 65% during test.
3. microbiological contamination: microbiological contamination atomisation pressure, spray time and bacterial aerosol state
With the microbiological contamination of full glass concentric circles sprayer, spray bacterium pressure is 2.5Kg/cm
2, spray bacterium time staphylococcus aureus is 10 seconds; Candida albicans is that two pipes sprayed 10 minutes simultaneously.
4. employing method, gas flow, sampling time, sampling location and height.
With full glass precursor solution impact sampler, sample at the fixed sample point of aerosol cabinet.Sampling flow is 10 liter/mins, and the sampling time is 1 minute.Sampled point is in the midpoint of aerosol cabinet height.
5. neutralizer test: adopt neutralisation and dilution method, will contain 5 * 10
5The neutralizer 10ml of cfu/ml bacteria suspension adds in the sampler.Test minute contrast combination experimental group.Experimental group is sprayed medicine to the used maximum dose in the aerosol cabinet, sample 1 minute be experimental group; Control group water spray post-sampling 1 minute carries out count plate with conventional method, and comparative test group and control group bacterium number have indifference.
6. bactericidal assay and number of repetition: experimental group and control group are established in test.Test under the same conditions.During test to 1m
3Spray bacterium colleague stirs in the aerosol cabinet, stops to spray bacterium and stirring to the scheduled time, static 1 minute, takes the control group sample.In the aerosol cabinet, spray medicine, clock, act on block sampling to the stipulated time, until reach till the final action time of regulation.The control group water spray, other same experimental group.Carry out count plate by method shown in " disinfection technology standard " (2.2), in 37 ℃ of insulating boxs, cultivate after 48 hours, observe end product.Control group calculates the Natural Decline rate, and experimental group is calculated killing rate.
The formal test triplicate.
Three, result
1. neutralizer test: result of the test shows, experimental group and control group bacterium amount be without significant difference, proves that this neutralizer can neutralize to adopt disinfectant as in the sampler.See the following form:
| The test sequence number | Control group bacterium number (cfu/ml) | Experimental group bacterium number (cfu/ml) |
| 1 | 2.32×10 5 | 2.29×10 5 |
| 2 | 2.01×10 5 | 2.04×10 5 |
| 3 | 1.89×10 5 | 1.97×10 5 |
| On average | 2.07×10 5 | 2.10×10 5 |
[0080]2. to the killing action of staphylococcus aureus in the air: at 1m
3Spray medicine 3.0 grams when acting on 15 minutes, all can reach 99.9% three times to airborne staphylococcus aureus killing rate in the aerosol cabinet; Killing rate is 21.21-37.80% in 99.9% Natural Decline rate.See the following form:
Annotate: negative control group (test is with batch common cultivation agar, PBS, common nutrient broth) is all without bacterial growth.
3. to the killing action of Candida albicans in the air: at 1m
3Spray medicine 4.3 grams when acting on 45 minutes, all can reach 100% three times to airborne Candida albicans killing rate in the aerosol cabinet; Killing rate is 38.65-56.66% in 100% Natural Decline rate.See the following form:
Annotate: negative control group (test is with batch common cultivation agar, PBS, common nutrient broth) is all without bacterial growth.
Four, conclusion:
1. to the killing effect of staphylococcus aureus in the air: at 1m
3In the aerosol cabinet, spray medicine 3.0 grams when acting on 15 minutes, all can reach 99.9% three times to airborne staphylococcus aureus killing rate.
2. to the killing effect of Candida albicans in the air: at 1m
3In the aerosol cabinet, spray medicine 4.3 grams when acting on 45 minutes, are 100% three times to airborne Candida albicans killing rate.
Embodiment 6:
After disinfectant warp let-off Shanghai City preventive medicine research institute (98) amount that the present invention makes is recognized (Shanghai) word (S0156) check, eye irritation test: nonirritant.Concrete test is as follows:
Submitted sample proterties: orange liquid.
The tested material preparation: stoste is directly tested.
Laboratory animal: new zealand rabbit, regular grade, four, body weight 2.5-3.0kg is provided by laboratory animal section of Shanghai Medical Univ, the quality certification number: 02-2-1.20 ± 2 ℃ of receptacle temperature, relative moisture 50-70%, animal housing's quality certification number: 02-28, feed for rabbit is provided by Su Hang Animal Science Development Co., Ltd, the quality certification number: 9905084.
Experimental technique:
1. test basis: disinfection technology standard (third edition)
2. animal left side eyelid is separated gently, in 2 pleasing to the eye conjunctival sacs of tested material, the physiological saline that the right side eye splashes into equivalent compares.
3. behind the catacleisis 4s, use normal saline flushing 5min about inciting somebody to action behind the eye drip.Respectively at behind the eye drip 1,24,48,72h and 4,7d, with the naked eye, hand-held crack lamp and 2% fluorescein check damage and the recovery situation of an eye conjunctiva, iris and cornea.
4. the grade scale score calculation day part eye that pressing acute ocular stimulates stimulates total mark average (index), estimates by the evaluation criterion of eye stimulus intensity with the peak of this index again.
Result of the test is as follows:
Conclusion:
Tested material is to eye irritant test of rabbit, and total mark average (the highest) is 2.0, belongs to nonirritant.
Embodiment 7:
Disinfectant of the present invention carries out following test as specimen.
One, mouse safety test:
The male 19-21g of Kunming mouse, totally 30, once oral specimen 0.05ml, 0.08ml, 0.1ml/ (all being configured to suspension with CMC only oral to 0.5ml/) observe a week.
The result: the oral 0.05ml/ of mouse only organizes and has no dead, and 0.08ml/ only organizes dead 3, and 0.1ml/ only organizes dead 5, show mouse once the maximal tolerance dose of oral disinfectant be 2.5ml/kg.
Two, mouse sucks safety test:
30 mouse divide three groups (male, 19-21g), with spray pattern (particle of spraying is even, is fine into vaporific) aim at mouse nostril spraying sterilizing agent 0.2ml/ only, 0.4ml/ only, 0.8ml/ only, once a day, totally three times, observe the mouse living condition.
The result: each organizes mouse all without unusual, also without dead, shows mouse in the spraying situation, and the maximum amount of feeling bad that sucks altogether is more than 40ml/kg.
Above demonstration and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (5)
1. greenweed medicinal herbs aerosol inhalation disinfectant, adopt herbal raw material to make disinfectant, it is characterized in that, described herbal raw material comprises following component: barberry 5%-15%, golden cypress 5%-15%, felwort 0%-10%, bark of ash 5%-15%, tree peony 5%-15%, Glabrous Sarcandra Herb 5%-15%, manyleaf meadowure rhizome and root 5%-15%, leatherleaf mahonia 10%-20%, Chinese mahonia leaf 5%-15%, honeysuckle 5%-15%.
2. greenweed medicinal herbs aerosol inhalation disinfectant according to claim 1, it is characterized in that, described herbal raw material comprises following component: barberry 10%, golden cypress 10%, felwort 5%, the bark of ash 10%, tree peony 10%, Glabrous Sarcandra Herb 10%, manyleaf meadowure rhizome and root 10%, leatherleaf mahonia 15%, Chinese mahonia leaf 10%, honeysuckle 10%.
3. greenweed medicinal herbs aerosol inhalation disinfectant according to claim 1 and 2 is characterized in that, described disinfectant adopts the mode of preserved material to be made by described Chinese herbal medicine.
4. greenweed medicinal herbs aerosol inhalation disinfectant according to claim 3 is characterized in that, comprises jamaicin 70% in the disinfectant that described herbal raw material is made.
5. greenweed medicinal herbs aerosol inhalation disinfectant according to claim 4 is characterized in that, also comprises essential oil 2% in the disinfectant that described herbal raw material is made.
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| CN105767048A (en) * | 2016-03-14 | 2016-07-20 | 申艳 | Special disinfection solution for injection room |
| CN106719147A (en) * | 2016-12-14 | 2017-05-31 | 五河县茂源水蛭生态养殖专业合作社 | A kind of breeding method of carp summer flower |
| CN112400905A (en) * | 2020-12-11 | 2021-02-26 | 陕西汤普医疗器械有限公司 | Acidic oxidation potential water antibacterial liquid and preparation method thereof |
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| CN1397168A (en) * | 2001-07-13 | 2003-02-19 | 周茜 | Composite disinfectant |
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| CN1397168A (en) * | 2001-07-13 | 2003-02-19 | 周茜 | Composite disinfectant |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105767048A (en) * | 2016-03-14 | 2016-07-20 | 申艳 | Special disinfection solution for injection room |
| CN106719147A (en) * | 2016-12-14 | 2017-05-31 | 五河县茂源水蛭生态养殖专业合作社 | A kind of breeding method of carp summer flower |
| CN112400905A (en) * | 2020-12-11 | 2021-02-26 | 陕西汤普医疗器械有限公司 | Acidic oxidation potential water antibacterial liquid and preparation method thereof |
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