CN103344615A - Application of tapered nanometer optical fiber, and micro-fluidic chip and preparation method thereof - Google Patents
Application of tapered nanometer optical fiber, and micro-fluidic chip and preparation method thereof Download PDFInfo
- Publication number
- CN103344615A CN103344615A CN2013102324458A CN201310232445A CN103344615A CN 103344615 A CN103344615 A CN 103344615A CN 2013102324458 A CN2013102324458 A CN 2013102324458A CN 201310232445 A CN201310232445 A CN 201310232445A CN 103344615 A CN103344615 A CN 103344615A
- Authority
- CN
- China
- Prior art keywords
- micro
- fluidic chip
- optical fibers
- nano optical
- nano
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention brings forward application of a tapered nanometer optical fiber. The smooth tapered nanometer optical fiber extends into a micro-channel of a micro fluidic chip substrate from a taper tip and is used as an optical waveguide for introduction of fluorescence exciting light of a to-be-detected object. According to a technical scheme provided by the invention, the tapered nanometer optical fiber is applied as the optical waveguide for introduction of fluorescence exciting light of the to-be-detected object, the taper tip of the nanometer optical fiber is inserted into the micro-channel of the micro fluidic chip substrate and corresponds to a designated to-be-detected point, the fluorescence exciting light irradiates and enters from a larger end of the nanometer optical fiber, and the nanometer optical fiber introduces the exciting light into the micro-channel and allows the exciting light to converge and then be shot out from the taper tip; fixed-point fluorescence excitation is carried out on the designated to-be-detected point, so interference of the exciting light on a fluorescence signal of a sample can be effectively avoided, and detection sensitivity and reliability are improved. The invention further brings forward a micro-fluidic chip and a preparation method thereof. The micro-fluidic chip not only has improved detection sensitivity and reliability but also enhances the integration level of a fluorescence excitation apparatus and the chip. The preparation method is simple, direct and highly efficient.
Description
Technical field
The present invention relates to the micro-fluidic chip detection technique that fields such as biology, chemistry utilize laser excitation material fluorescence, be specifically related to application process, micro-fluidic chip of a kind of taper nano optical fibers and preparation method thereof.
Background technology
Use the fluorescence of laser excitation detection material, to sample carry out composition, concentration analysis is a kind of important biological chemistry detection means.Micro-fluidic chip can be integrated in basic operation units such as the specimen preparation in the biological and chemical field, biochemical reaction, separation, detection on the chip of centimetre magnitude.
In recent years, in micro-fluidic field, along with improving constantly that microflow control technique requires, the microchannel size of micro-fluidic chip is progressively dwindled.Yet, concerning the micro-fluidic chip that the microchannel size is further dwindled, adopt the mode of conventional laser direct irradiation, or go into the method for optical fiber at chip buried-in, all can be owing to the fluorescence excitation device size is excessive, plane of illumination disturbs the fluorescence signal of the sample in the microchannel greatly, thereby influences detection sensitivity and the reliability of sample; In addition, the mode of excitation of direct irradiation becomes two independently experimental considerations units with fluorescence excitation device and micro-fluidic chip are discrete, greatly reduces the integrated level of the detection system of whole application micro-fluidic chip.Therefore, how more effective, can in the microchannel of micro-fluidic chip, realize the fixed point fluorescence excitation, improving sensitivity and the reliability of sample detection, and strengthen the integrated level of detection system integral body, become and use microflow control technique and carry out a problem demanding prompt solution in the material detection range.
Given this, this case the inventor further investigate the problems referred to above, has this case to produce then.
Summary of the invention
One purpose of the present invention is to provide a kind of application that can effectively avoid the taper nano optical fibers of exciting light disturbed specimen fluorescence signal, has improved sensitivity and the reliability of sample detection.
Its two purpose of the present invention is to provide a kind of formed micro-fluidic chip of application that relies on above-mentioned taper nano optical fibers, with when improving detection sensitivity and reliability, strengthen the integrated level of the whole detection system of formations such as fluorescence excitation device and micro-fluidic chip substrate, micro-fluidic chip cover plate.
Its three purpose of the present invention is to provide a kind of preparation method who prepares above-mentioned micro-fluidic chip, and the method can efficiently realize the preparation of micro-fluidic chip, and is directly simple.
In order to achieve the above object, the present invention adopts following technical scheme:
The optical waveguide as the exciting light of detected material fluorescence is stretched into the nano optical fibers of smooth tapered in the microchannel of micro-fluidic chip substrate in a kind of application of taper nano optical fibers by the awl point.
The nano wire of being made by high molecular polymer in the cone tip part setting of above-mentioned nano optical fibers, behind the appointment tested point in the awl point of described nano optical fibers extends the microchannel of micro-fluidic chip substrate, melt described nano wire, described nano optical fibers fixedly is bonded in the microchannel of micro-fluidic chip substrate.
Employing can be melted effect to nano wire with the steam that above-mentioned nano wire melts.
Adopt chloroform vapors that above-mentioned nano wire is melted effect.
By the medical catheter that is communicated with the chloroform vapors source of the gas the above-mentioned chloroform vapors above-mentioned nano wire that leads is melted effect.
The formed micro-fluidic chip of a kind of application of taper nano optical fibers, comprise micro-fluidic chip substrate, micro-fluidic chip cover plate and above-mentioned nano optical fibers, the awl point of described nano optical fibers extends the appointment tested point place in the microchannel of described micro-fluidic chip substrate and is connected and fixed; Described micro-fluidic chip cover plate is corresponding with described micro-fluidic chip sizes of substrate, and covers on the micro-fluidic chip substrate.
A kind of preparation method of the formed micro-fluidic chip of application of taper nano optical fibers:
(1) adopt flame fused biconical taper method standard single-mode fiber to be drawn into the above-mentioned nano optical fibers of level and smooth taper;
(2) under optical microscope, above-mentioned nano optical fibers is inserted in the microchannel of above-mentioned micro-fluidic chip substrate the corresponding check point of specifying of awl point by three-dimensional micropositioning stage;
(3) above-mentioned nano optical fibers is fixedly connected in the microchannel of above-mentioned micro-fluidic chip substrate;
(4) after above-mentioned nano optical fibers is fixedly connected on the above-mentioned micro-fluidic chip substrate, install above-mentioned micro-fluidic chip cover plate additional.
After adopting technique scheme, the application of taper nano optical fibers of the present invention, it breaks through the form that traditional micro-fluidic chip is used the fluorescence excitation device, its awl point by nano optical fibers stretches in the microchannel of micro-fluidic chip substrate the optical waveguide as the exciting light of detected material fluorescence, and the corresponding tested point of specifying of awl point, the exciting light of fluorescence is entered by the butt end irradiation of nano optical fibers, obtain the hot spot of sub-wavelength or sub-micrometer scale at cone tip part, above-mentioned appointment tested point is carried out the fixed point fluorescence excitation of high concentration.Compared with prior art, the application of taper nano optical fibers of the present invention, its excitation light that has overcome the conventional fluorescent excitation apparatus big defective of showing up, can effectively avoid exciting light to the interference of fluorescent signal, improve the sensitivity and the reliability that detect, by the strong evanscent field of nano optical fibers awl point, can effectively realize the detection of micro-example simultaneously.
The present invention also proposes a kind of formed micro-fluidic chip of application that relies on above-mentioned taper nano optical fibers, it is when improving detection sensitivity and reliability, also strengthened the integrated level of the whole detection system that fluorescence excitation device and micro-fluidic chip substrate, micro-fluidic chip cover plate etc. constitute, simple and reasonable, be beneficial to and produce and apply.
The present invention also proposes a kind of preparation method who prepares above-mentioned micro-fluidic chip, and the method can efficiently realize adopting the taper nano optical fibers to carry out the preparation of the micro-fluidic chip of fluorescence excitation, and is simple to operate direct.
Description of drawings
Fig. 1 is the structural representation of nano optical fibers;
Fig. 2 is that nano optical fibers is combined the structural representation of state with nano wire;
Fig. 3 is the application state synoptic diagram of taper nano optical fibers;
Fig. 4 is microfluidic chip structure synoptic diagram of the present invention.
Among the figure:
1-nano optical fibers 11-bores point
12-butt end 2-nano wire
3-micro-fluidic chip substrate 31-microchannel
4-medical catheter 41-gas outlet
5-micro-fluidic chip cover plate
Embodiment
Below, by specific embodiment, further explained in detail technical scheme of the present invention.
The application of a kind of taper nano optical fibers of the present invention as Figure 1-3, is prepared the nano optical fibers 1 of smooth tapered.
The nano optical fibers 1 of smooth tapered is stretched in the microchannel 31 of micro-fluidic chip substrate 3 by awl point 11, as the optical waveguide of the exciting light that imports detected material fluorescence.When concrete the application, butt end 12 irradiations by nano optical fibers 1 of the exciting light of fluorescence are entered, nano optical fibers 1 can import exciting light in the microchannel 31 and concentrate on bores the ejaculation of sharp 11 places, because exciting light is at the tangible evanscent field of nano optical fibers 1 outside formation, and awl point 11 has enhancement effect, make the light intensity at sharp 11 places of awl obviously increase, obtain the hot spot of sub-wavelength or sub-micrometer scale at sharp 11 places of awl, above-mentioned appointment tested point is carried out the fixed point fluorescence excitation of high concentration, the excitation light that the has overcome the conventional fluorescent excitation apparatus big defective of showing up can effectively avoid exciting light that the fluorescent signal is disturbed.
In order to realize the application of nano optical fibers 1, preferably, 11 places arrange nano wire 2 at the awl of nano optical fibers 1 point, and nano wire 2 is made by high molecular polymer, and heating is easily melted, and at room temperature easily condenses.Specifically be, nano wire 2 is connected in awl point 11 places of nano optical fibers 1 near most advanced and sophisticated position, behind the appointment tested point in awl point 11 extends the microchannel 31 of micro-fluidic chip substrate 3, thawing nano wire 2 fixedly is bonded in nano optical fibers 1 in the microchannel 31 after nano wire 2 condenses.
Particularly, employing can be melted effect to nano wire 2 with the steam that nano wire 2 melts.
Further, adopt chloroform vapors that nano wire 2 is melted effect.
Preferably, with the chloroform vapors nano wire 2 that leads, melt effect by the medical catheter 4 that is communicated with the chloroform vapors source of the gas.
A kind of micro-fluidic chip of the present invention, as shown in Figure 4, comprise the nano optical fibers 1 in micro-fluidic chip substrate 3, micro-fluidic chip cover plate 5 and aforementioned each embodiment, the awl point 11 of nano optical fibers 1 extends the appointment tested point place in the microchannel 31 of micro-fluidic chip substrate 3 and is connected and fixed; Micro-fluidic chip cover plate 5 is corresponding with micro-fluidic chip substrate 3 sizes, and covers on micro-fluidic chip substrate 3.Particularly, adopt traditional micro-flow control chip preparation method, obtain micro-fluidic chip substrate 3 and micro-fluidic chip cover plate 5, standby; Nano optical fibers 1 is bonded together by the nano wire 2 that solidifies again after being melted by steam with micro-fluidic chip substrate 3, it possesses the function that improves detection sensitivity and reliability, has also strengthened the integrated level of the whole detection system that fluorescence excitation device and micro-fluidic chip substrate 3, micro-fluidic chip cover plate 5 constitute simultaneously.
For the smooth tapered of specific implementation nano optical fibers 1, preferably, nano optical fibers 1 adopts flame fused biconical taper method to be prepared from.
In order to guarantee light propagation efficiency and the performance of nano optical fibers 1, and guarantee emergent light effectively and intensity, preferably, nano optical fibers 1 is made by standard single-mode fiber, and butt end 12 is corresponding with the standard single-mode fiber diameter, and boring sharp 11 diameters is 20-50nm, and drift angle is 15-30 °.
For the function of specific implementation nano wire 2, preferably, nano wire 2 is made by polystyrene material, and diameter is 350-450nm, and length is 5-15 μ m.
For the preparation of specific implementation nano wire 2, preferably, nano wire 2 adopts the mixed solution pulling method to be prepared from.
Thawing for specific implementation nano wire 2, preferably, also comprise the medical catheter 4 that is connected with the chloroform vapors source of the gas, the nano wire 2 that gas outlet 41 correspondences of this medical catheter 4 are in sharp 11 places of awl arranges, and medical catheter 4 melts effect with the chloroform vapors nano wire 2 that leads.
The preparation method of a kind of micro-fluidic chip of the present invention:
(1) adopt flame fused biconical taper method standard single-mode fiber to be drawn into the nano optical fibers 1 of smooth tapered; When need arrange nano wire 2, adopt the mixed solution pulling method to draw the nano wire 2 of high molecular polymer; Under optical microscope, choose the nano wire 2 of corresponding size, concrete optional cut-off is the polymer nano rice noodles 2 of 10 μ m for 400nm, length directly; The nano wire of choosing 2 is placed awl point 11 places of nano optical fibers 1 by the micro-nano operation;
(2) under optical microscope,, nano optical fibers 1 is inserted in the microchannel 31 of micro-fluidic chip substrate 3 the awl point 11 corresponding check points of specifying by the control of three-dimensional micropositioning stage;
(3) nano optical fibers 1 is fixedly connected in the microchannel 31 of micro-fluidic chip substrate 3.
(4) in order to make micro-fluidic chip more complete, after nano optical fibers 1 fixedly is bonded on the micro-fluidic chip substrate 3, install micro-fluidic chip cover plate 5 additional.
When need adopt chloroform vapors to melt nano wire 2, the medical catheter 4 of corresponding size is connected with the chloroform vapors source of the gas, and gas outlet 41 correspondences of medical catheter 4 are in the nano wire 2 at awl point 11 places of nano optical fibers 1, specifically can be: medical catheter 4 is positioned at sharp 11 tops of awl, apart from micro-fluidic chip substrate 3 about 1 μ m places, gas outlet 41 is over against nano wire 2; Start above-mentioned chloroform vapors source of the gas, chloroform vapors blows to nano wire 2 by medical catheter 4 and with its thawing; After nano wire 2 melts and is boring sharp 11 places formation macromolecule polymer solution, to withdraw medical catheter 4 in good time or close the chloroform vapors source of the gas, polymer solution can solidify gradually, and nano optical fibers 1 fixedly is bonded on the micro-fluidic chip substrate 3.
If when adopting complete standard single-mode fiber to prepare nano optical fibers 1, should before drawing awl, at first remove the coat of standard single-mode fiber.
The application of taper nano optical fibers of the present invention, micro-fluidic chip and preparation method thereof, micro-fluidic chip substrate are preferably and adopt the dimethyl silicone polymer material to make, and also can require to select for use other appropriate materials according to reality; Preferably after nano optical fibers fixedly is bonded on the micro-fluidic chip substrate, install the micro-fluidic chip cover plate additional, and the overall dimensions of micro-fluidic chip cover plate is corresponding with the micro-fluidic chip substrate, its material and concrete form can require to adjust and design according to reality; In the micro-fluidic chip substrate, the width of microchannel and the degree of depth are preferably about 25 μ m, and length is about 1cm; Medical catheter is preferably about internal diameter 1mm, acts on the nano wire approximately it to be melted about 5s, withdraws medical catheter then, closes the chloroform vapors source of the gas, also can only close the chloroform vapors source of the gas under the situation that does not influence follow-up work; Be preferably and use medical catheter to carry chloroform vapors that nano wire is melted, also can require to adopt other suitable steam or conduit form according to reality, in broad terms, also can adopt the existing suitable means outside the steam that nano wire is melted; The corresponding appointment of the awl point tested point setting of nano optical fibers, according to the difference of specifying tested point, the position that arranges of awl point and whole nano optical fibers also changes thereupon; Nano wire is preferably polystyrene material, adopts the preparation of mixed solution pulling method, and diameter is about 400nm, and length is about 10 μ m, also can require to adopt other suitable macromolecule polymer material, preparation method and choose other size according to reality; Nano optical fibers preferably adopts standard single mode SiO
2Optical fiber is made, and boring sharp diameter is 20-50nm, and drift angle is 15-30 °, also can require to select for use other suitable nano optical fibers material and choose other size according to reality; For guaranteeing that nano optical fibers is smooth tapered, be preferably the preparation of employing flame fused biconical taper method, also can require to select other existing suitable preparation method according to reality, and the butt end of nano optical fibers can require carry out the equal diameter extension as length requirement according to reality.
Product form of the present invention is not limited to this case diagram and embodiment, and anyone carries out suitable variation or the modification of similar thinking to it, all should be considered as not breaking away from patent category of the present invention.
Claims (7)
1. the application of a taper nano optical fibers is characterized in that: the nano optical fibers of smooth tapered is stretched in the microchannel of micro-fluidic chip substrate by the awl point, as the optical waveguide that imports the detected material fluorescent exciting.
2. the application of a kind of taper nano optical fibers according to claim 1, it is characterized in that: the nano wire of being made by high molecular polymer in the cone tip part setting of above-mentioned nano optical fibers, behind the appointment tested point in the awl point of described nano optical fibers extends the microchannel of micro-fluidic chip substrate, melt described nano wire, described nano optical fibers fixedly is bonded in the microchannel of micro-fluidic chip substrate.
3. the application of a kind of taper nano optical fibers according to claim 2 is characterized in that: employing can be melted effect to nano wire with the steam that above-mentioned nano wire melts.
4. the application of a kind of taper nano optical fibers according to claim 3 is characterized in that: adopt chloroform vapors that above-mentioned nano wire is melted effect.
5. the application of a kind of taper nano optical fibers according to claim 4 is characterized in that: by the medical catheter that is communicated with the chloroform vapors source of the gas the above-mentioned chloroform vapors above-mentioned nano wire that leads is melted effect.
6. according to the formed micro-fluidic chip of application of each described a kind of taper nano optical fibers among the claim 1-5, it is characterized in that: comprise micro-fluidic chip substrate, micro-fluidic chip cover plate and above-mentioned nano optical fibers, the awl point of described nano optical fibers extends the appointment tested point place in the microchannel of described micro-fluidic chip substrate and is connected and fixed; Described micro-fluidic chip cover plate is corresponding with described micro-fluidic chip sizes of substrate, and covers on the micro-fluidic chip substrate.
7. the preparation method of the formed micro-fluidic chip of application of a kind of taper nano optical fibers according to claim 6 is characterized in that:
(1) adopt flame fused biconical taper method standard single-mode fiber to be drawn into the above-mentioned nano optical fibers of level and smooth taper;
(2) under optical microscope, above-mentioned nano optical fibers is inserted in the microchannel of above-mentioned micro-fluidic chip substrate the corresponding check point of specifying of awl point by three-dimensional micropositioning stage;
(3) above-mentioned nano optical fibers is fixedly connected in the microchannel of above-mentioned micro-fluidic chip substrate;
(4) after above-mentioned nano optical fibers is fixedly connected on the above-mentioned micro-fluidic chip substrate, install above-mentioned micro-fluidic chip cover plate additional.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310232445.8A CN103344615B (en) | 2013-06-09 | 2013-06-09 | Micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310232445.8A CN103344615B (en) | 2013-06-09 | 2013-06-09 | Micro-fluidic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103344615A true CN103344615A (en) | 2013-10-09 |
| CN103344615B CN103344615B (en) | 2015-05-20 |
Family
ID=49279429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310232445.8A Expired - Fee Related CN103344615B (en) | 2013-06-09 | 2013-06-09 | Micro-fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103344615B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104897654A (en) * | 2015-06-02 | 2015-09-09 | 北京纳迅科技有限公司 | Detection device for micro-fluidic biologic chip and preparation method of detection device |
| US9518923B1 (en) | 2015-12-07 | 2016-12-13 | International Business Machines Corporation | System and methods for fluorescence detection |
| CN109283617A (en) * | 2018-11-30 | 2019-01-29 | 华侨大学 | A kind of miniature multicolor displaying linear light source and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183462A (en) * | 2011-03-24 | 2011-09-14 | 浙江大学 | Embedded type fiber taper nanofiber micro-fluidic control chip sensor and production method thereof |
-
2013
- 2013-06-09 CN CN201310232445.8A patent/CN103344615B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183462A (en) * | 2011-03-24 | 2011-09-14 | 浙江大学 | Embedded type fiber taper nanofiber micro-fluidic control chip sensor and production method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张威等: "半导体激光器组件封装中光纤的固定方法", 《半导体光电》, vol. 25, no. 2, 31 December 2004 (2004-12-31) * |
| 徐颖颖: "非绝热锥形微纳光纤的多模效应及其在微混合器中的应用", 《中国优秀硕士学位论文全文数据库》, no. 07, 15 July 2011 (2011-07-15) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104897654A (en) * | 2015-06-02 | 2015-09-09 | 北京纳迅科技有限公司 | Detection device for micro-fluidic biologic chip and preparation method of detection device |
| CN104897654B (en) * | 2015-06-02 | 2018-06-01 | 北京纳迅科技股份有限公司 | A kind of microflow controlled biochip detection device and preparation method |
| US9518923B1 (en) | 2015-12-07 | 2016-12-13 | International Business Machines Corporation | System and methods for fluorescence detection |
| CN109283617A (en) * | 2018-11-30 | 2019-01-29 | 华侨大学 | A kind of miniature multicolor displaying linear light source and preparation method thereof |
| CN109283617B (en) * | 2018-11-30 | 2024-02-27 | 华侨大学 | Miniature multicolor display line light source and manufacturing method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103344615B (en) | 2015-05-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102183462B (en) | Embedded type fiber taper nanofiber micro-fluidic control chip sensor and production method thereof | |
| JP2016512427A5 (en) | ||
| US20160016180A1 (en) | Devices, systems, and methods for acoustically-enhanced magnetophoresis | |
| CN102240534A (en) | Method for manufacturing three-dimensional micromixer microfluidic chip | |
| CN103344615B (en) | Micro-fluidic chip | |
| JP2005204661A (en) | Detecting apparatus for molecule derived from organism, dioxins and endocrine disrupter, and method for detection using the same | |
| CN103409317A (en) | Capillary bioanalysis system, and analytical method and applications thereof | |
| CN106215986A (en) | A kind of PDMS microfluidic chip structure and preparation method thereof | |
| CN104745446A (en) | PCR (Photo-conductive Relay) and capillary electrophoresis integrated micro-fluidic chip and rapid pathogenic bacteria detection device adopting chip | |
| CN103316723A (en) | Microfluidic electrophoresis chip | |
| Lv et al. | A precise pointing nanopipette for single-cell imaging via electroosmotic injection | |
| CN103323439B (en) | A kind of micro-fluidic chip fluorescence excitation device, micro-fluidic chip and preparation method thereof | |
| CN105842324A (en) | Integrated micro-fluidic chip and working method thereof | |
| JP2010117197A (en) | Microparticle dispenser and microparticle dispensing method | |
| Li et al. | Optical separation and controllable delivery of cells from particle and cell mixture | |
| CN103926190A (en) | Automatic single cell analysis method based on microfluidic system | |
| CN100578210C (en) | Microvolume sample lead in device used in capillary tube electrophoresis and use method thereof | |
| CN208921621U (en) | A kind of fluorescent chemicals miniflow detector based on Hollow-Core Photonic Crystal Fibers | |
| CN203342823U (en) | Micro-fluidic chip | |
| CN203324191U (en) | Micro-fluidic chip fluorescence excitation device and micro-fluidic chip | |
| CN107831811A (en) | The fluid channel flow control apparatus and control method of a kind of micro-nano cellulose | |
| Alrifaiy et al. | How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte | |
| EP4141099A1 (en) | Cell sorting chip, apparatus, and method based on dielectric deterministic displacement | |
| CN103698382A (en) | Capillary electrophoresis analysis device for trace droplet array and application method thereof | |
| JP2012018039A (en) | Microchannel, and microchip, column, device, and method for nucleic acid hybridization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150520 |