CN203342823U

CN203342823U - Micro-fluidic chip

Info

Publication number: CN203342823U
Application number: CN 201320336689
Authority: CN
Inventors: 张奚宁; 童利民; 戴昊; 胡志方
Original assignee: Huaqiao University
Current assignee: Huaqiao University
Priority date: 2013-06-09
Filing date: 2013-06-09
Publication date: 2013-12-18
Anticipated expiration: 2023-06-09

Abstract

The utility model provides a micro-fluidic chip comprising a micro-fluidic chip substrate, a micro-fluidic chip cover sheet and smooth conical nanometer optical fiber, wherein a conical tip of the nanometer optical fiber stretches into an appointed to-be-detected point in a microchannel of the micro-fluidic chip substrate and is connected and fixed; the size of the micro-fluidic chip cover sheet corresponds to that of the micro-fluidic chip substrate; and the micro-fluidic chip cover sheet covers the micro-fluidic chip substrate. After the micro-fluidic chip provided by the utility model adopts the technical scheme, the nanometer optical fiber serves as optical waveguide of fluorescence excitation light which is guided into a detected object, the conical tip of the nanometer optical fiber stretches into the corresponding appointed to-be-detected point in the microchannel of the micro-fluidic chip substrate, the excitation light of the fluorescence enters by irradiating the thick end of the nanometer optical fiber, and the excitation light is guided into the microchannel by the nanometer optical and is concentrated at the conical tip to be emitted, and the appointed to-be-detected point is subjected to fixed-point fluorescence excitation, so that the interference of the excitation light on a fluorescent signal of a sample can be effectively avoided, detection sensitivity and reliability are improved, and the integration degree of a fluorescence excitation device and the chip is enhanced.

Description

A kind of micro-fluidic chip

Technical field

The utility model relates to the micro-fluidic chip detection technique that the fields such as biology, chemistry utilize laser excitation material fluorescence, is specifically related to a kind of micro-fluidic chip.

Background technology

The fluorescence of application laser excitation detection material, to sample carry out composition, concentration analysis is a kind of important biochemistry detection means.Micro-fluidic chip can be integrated in the basic operation units such as the sample preparation in the biological and chemical field, biochemical reaction, separation, detection on the chip of centimetre magnitude.

In recent years, in micro-fluidic field, along with improving constantly that microflow control technique requires, the microchannel size of micro-fluidic chip is progressively dwindled.Yet, the micro-fluidic chip that the microchannel size is further dwindled, adopt the mode of conventional laser direct irradiation, or enter the method for optical fiber at chip buried-in, all can be due to the fluorescence excitation device oversize, plane of illumination disturbs greatly the fluorescence signal of the sample in microchannel, thereby affects detection sensitivity and the reliability of sample; In addition, the mode of excitation of direct irradiation, become two independently experimental considerations units by fluorescence excitation device and micro-fluidic chip are discrete, greatly reduces the integrated level of the detection system of whole application micro-fluidic chip.Therefore, how more effective, can in the microchannel of micro-fluidic chip, realize the fixed point fluorescence excitation, to improve sensitivity and the reliability of sample detection, and strengthen the integrated level of detection system integral body, become the application microflow control technique and carry out a problem demanding prompt solution in the material detection field.

Given this, this case the inventor furtherd investigate the problems referred to above, then has this case to produce.

The utility model content

The purpose of this utility model is to provide a kind of micro-fluidic chip, and it strengthens the integrated level of the whole detection system of the formations such as fluorescence excitation device and micro-fluidic chip substrate, micro-fluidic chip cover plate when improving detection sensitivity and reliability.

In order to achieve the above object, the utility model adopts following technical scheme:

A kind of micro-fluidic chip, comprise that the nano optical fibers of micro-fluidic chip substrate, micro-fluidic chip cover plate and smooth tapered, the cone point of described nano optical fibers extend the appointment tested point place in the microchannel of described micro-fluidic chip substrate and be connected and fixed; Described micro-fluidic chip cover plate is corresponding with described micro-fluidic chip sizes of substrate, and covers on the micro-fluidic chip substrate.

After adopting technique scheme, micro-fluidic chip of the present utility model, it breaks through the structural form of traditional micro-fluidic chip application fluorescence excitation device, its point of cone by nano optical fibers stretches in the microchannel of micro-fluidic chip substrate the fiber waveguide as the exciting light of detected material fluorescence, and the corresponding tested point of specifying of cone point, the exciting light of fluorescence is entered by the butt end irradiation of nano optical fibers, obtain the hot spot of sub-wavelength or sub-micrometer scale at cone tip part, above-mentioned appointment tested point is carried out to the fixed point fluorescence excitation of high concentration.Compared with prior art, micro-fluidic chip of the present utility model, its excitation light that has overcome the conventional fluorescent excitation apparatus large defect of showing up, can effectively avoid the interference of exciting light to the fluorescent signal, improve the sensitivity and the reliability that detect, bore the strong evanscent field of point by nano optical fibers simultaneously, can effectively realize the detection of micro-example, also strengthened the integrated level of the whole detection system that fluorescence excitation device and micro-fluidic chip substrate, micro-fluidic chip cover plate etc. form, simple and reasonable, be beneficial to and produce and apply.

The accompanying drawing explanation

The structural representation that Fig. 1 is nano optical fibers;

Fig. 2 is that nano optical fibers is combined the structural representation of state with nano wire;

The application state schematic diagram that Fig. 3 is the taper nano optical fibers;

Fig. 4 is the utility model microfluidic chip structure schematic diagram.

In figure:

1-nano optical fibers 11-bores point

12-butt end 2-nano wire

3-micro-fluidic chip substrate 31-microchannel

4-medical catheter 41-gas outlet

5-micro-fluidic chip cover plate

The specific embodiment

Below, by specific embodiment, further explain in detail the technical solution of the utility model.

A kind of micro-fluidic chip of the present utility model, as Figure 1-4, prepare the nano optical fibers 1 of smooth tapered.

The nano optical fibers of smooth tapered 1 is stretched in the microchannel 31 of micro-fluidic chip substrate 3 by cone point 11, as the fiber waveguide of the exciting light that imports detected material fluorescence.When concrete application, butt end 12 irradiations by the exciting light of fluorescence by nano optical fibers 1 enter, nano optical fibers 1 can import exciting light in microchannel 31 and concentrate on bores sharp 11 places ejaculation, because exciting light forms obvious evanscent field in nano optical fibers 1 outside, and cone point 11 has enhancement effect, make the light intensity at sharp 11 places of cone obviously increase, obtain the hot spot of sub-wavelength or sub-micrometer scale at sharp 11 places of cone, above-mentioned appointment tested point is carried out to the fixed point fluorescence excitation of high concentration, the excitation light that the has overcome the conventional fluorescent excitation apparatus large defect of showing up, can effectively avoid exciting light to be disturbed the fluorescent signal.

, preferably, at cone point 11 places of nano optical fibers 1, nano wire 2 being set, nano wire 2 is made by high molecular polymer, and heating is easily melted, and at room temperature easily condenses.Specifically, nano wire 2 is connected in cone point 11 places of nano optical fibers 1 by subapical position, after appointment tested point in cone point 11 extends the microchannel 31 of micro-fluidic chip substrate 3, thawing nano wire 2 fixedly is bonded in nano optical fibers 1 in microchannel 31 after nano wire 2 condenses.

Particularly, the steam that employing can be melted nano wire 2 carries out the thawing effect to nano wire 2.

Further, adopt chloroform vapors to carry out the thawing effect to nano wire 2.

Preferably, by the chloroform vapors nano wire 2 that leads, carry out the thawing effect by the medical catheter 4 that is communicated with the chloroform vapors source of the gas.

A kind of micro-fluidic chip of the present utility model, in the specific implementation, it comprises the nano optical fibers 1 of the taper in micro-fluidic chip substrate 3, micro-fluidic chip cover plate 5 and aforementioned each embodiment, and the cone point 11 of nano optical fibers 1 extends the appointment tested point place in the microchannel 31 of micro-fluidic chip substrate 3 and is connected and fixed; Micro-fluidic chip cover plate 5 is corresponding with micro-fluidic chip substrate 3 sizes, and covers on micro-fluidic chip substrate 3.Particularly, adopt traditional micro-flow control chip preparation method, obtain micro-fluidic chip substrate 3 and micro-fluidic chip cover plate 5, standby; Nano optical fibers 1 is bonded together by the nano wire 2 solidified again after being melted by steam with micro-fluidic chip substrate 3, it possesses the function that improves detection sensitivity and reliability, has also strengthened the integrated level of the whole detection system that fluorescence excitation device and micro-fluidic chip substrate 3, micro-fluidic chip cover plate 5 form simultaneously.

For the smooth tapered of specific implementation nano optical fibers 1, preferably, nano optical fibers 1 adopts flame fused biconical taper legal system for forming.

In order to guarantee light propagation efficiency and the performance of nano optical fibers 1, and the effective and intensity of assurance emergent light, preferably, nano optical fibers 1 is made by standard single-mode fiber, and butt end 12 is corresponding with the standard single-mode fiber diameter, boring sharp 11 diameters is 20-50nm, and drift angle is 15-30 °.

For the function of specific implementation nano wire 2, preferably, nano wire 2 is made by polystyrene material, and diameter is 350-450nm, and length is 5-15 μ m.

For the preparation of specific implementation nano wire 2, preferably, nano wire 2 adopts the mixed solution pulling method to be prepared from.

Thawing for specific implementation nano wire 2, preferably, also comprise the medical catheter 4 be connected with the chloroform vapors source of the gas, the nano wire of gas outlet 41 correspondences of this medical catheter 4 in boring sharp 11 places 2 arranges, and medical catheter 4 carries out the thawing effect by the chloroform vapors nano wire 2 that leads.

The preparation method of a kind of micro-fluidic chip of the present utility model:

(1) adopt flame fused biconical taper method standard single-mode fiber to be drawn into to the nano optical fibers 1 of smooth tapered; When need arrange nano wire 2, adopt the mixed solution pulling method to draw the nano wire 2 of high molecular polymer; Under light microscope, choose the nano wire 2 of corresponding size, concrete optional cut-off footpath is the polymer nano rice noodles 2 that 400nm, length are 10 μ m; Operate by micro-nano cone point 11 places that the nano wire 2 that will choose is placed in nano optical fibers 1;

(2), under light microscope, by the control of three-dimensional fine-tuning frame, nano optical fibers 1 is inserted in the microchannel 31 of micro-fluidic chip substrate 3 to the corresponding test point of specifying of cone point 11;

(3) nano optical fibers 1 is fixedly connected in the microchannel 31 of micro-fluidic chip substrate 3.

(4), in order to make micro-fluidic chip more complete, after nano optical fibers 1 fixedly is bonded on micro-fluidic chip substrate 3, install micro-fluidic chip cover plate 5 additional.

When need adopt chloroform vapors to melt nano wire 2, the medical catheter of corresponding size 4 is connected with the chloroform vapors source of the gas, and by the gas outlet of medical catheter 4 41 correspondences the nano wire 2 at the cone in nano optical fibers 1 point 11 places, specifically can be: medical catheter 4 is positioned at sharp 11 tops of cone, apart from the about 1 μ m place of micro-fluidic chip substrate 3, gas outlet 41 is over against nano wire 2; Start above-mentioned chloroform vapors source of the gas, chloroform vapors blows to nano wire 2 by medical catheter 4 and by its thawing; When nano wire 2 melts and, after sharp 11 places of cone form macromolecule polymer solution, withdraws medical catheter 4 or close the chloroform vapors source of the gas in good time, polymer solution can solidify gradually, and nano optical fibers 1 fixedly is bonded on micro-fluidic chip substrate 3.

If while adopting complete standard single-mode fiber to prepare nano optical fibers 1, should, before being drawn cone, at first remove the coat of standard single-mode fiber.

Micro-fluidic chip of the present utility model, the micro-fluidic chip substrate is preferably and adopts the dimethyl silicone polymer material to make, and also can select other appropriate materials according to actual requirement; Preferably, after nano optical fibers fixedly is bonded on the micro-fluidic chip substrate, install the micro-fluidic chip cover plate additional, and the overall dimensions of micro-fluidic chip cover plate is corresponding with the micro-fluidic chip substrate, its material and concrete form can be adjusted and be designed according to actual requirement; In the micro-fluidic chip substrate, the width of microchannel and the degree of depth are preferably 25 μ m left and right, and length is about 1cm; Medical catheter is preferably about internal diameter 1mm, and acting on nano wire approximately 5s left and right can melt it, then withdraws medical catheter, closes the chloroform vapors source of the gas, also can in the situation that do not affect follow-up work, only close the chloroform vapors source of the gas; Be preferably and use medical catheter to carry chloroform vapors that nano wire is melted, also can adopt other suitable steam or conduit form according to actual requirement, in broad terms, also can adopt the existing suitable means outside steam that nano wire is melted; The corresponding appointment of the cone point tested point setting of nano optical fibers, according to the difference of specifying tested point, the setting position of cone point and whole nano optical fibers also changes thereupon; Nano wire is preferably polystyrene material, adopts the preparation of mixed solution pulling method, and diameter is about 400nm, and length is about 10 μ m, also can adopt other suitable macromolecule polymer material, preparation method and choose other size according to actual requirement; Nano optical fibers preferably adopts standard single mode SiO ₂optical fiber is made, and boring sharp diameter is 20-50nm, and drift angle is 15-30 °, also can select other suitable nano optical fibers material and choose other size according to actual requirement; For guaranteeing that nano optical fibers is smooth tapered, be preferably and adopt flame fused biconical taper legal system standby, also can select other existing suitable preparation method according to actual requirement, and the butt end of nano optical fibers can carry out the equal diameter extension as length requirement according to actual requirement.

Product form of the present utility model is not limited to this case diagram and embodiment, and anyone carries out suitable variation or the modification of similar thinking to it, all should be considered as not breaking away from patent category of the present utility model.

Claims

1. a micro-fluidic chip, it is characterized in that: comprise the nano optical fibers of micro-fluidic chip substrate, micro-fluidic chip cover plate and smooth tapered, the cone point of described nano optical fibers extends the appointment tested point place in the microchannel of described micro-fluidic chip substrate and is connected and fixed; Described micro-fluidic chip cover plate is corresponding with described micro-fluidic chip sizes of substrate, and covers on the micro-fluidic chip substrate.