CN203778087U - Micro-fluidic chip and cell apoptosis detection system based on same - Google Patents

Micro-fluidic chip and cell apoptosis detection system based on same Download PDF

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Publication number
CN203778087U
CN203778087U CN201320877797.4U CN201320877797U CN203778087U CN 203778087 U CN203778087 U CN 203778087U CN 201320877797 U CN201320877797 U CN 201320877797U CN 203778087 U CN203778087 U CN 203778087U
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micro
fluidic chip
detection
light source
nutrient solution
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陈建军
单鹏飞
高贻钧
丁孺牛
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Huazhong Agricultural University
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Huazhong Agricultural University
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Abstract

The utility model discloses a micro-fluidic chip. The micro-fluidic chip comprises a culture solution channel and a detection solution channel, wherein the culture solution channel consists of a culture solution sampling hole, a culture solution channel body and detection holes; the detection solution channel consists of a detection solution sampling hole, a detection solution channel body and mixing holes; the mixing holes are in one-to-one correspondence to the detection holes, and are communicated with the detection solution channel body and the detection holes, respectively; a culture solution and a detection reagent encounter each other and are mixed with each other in the mixing holes. The utility model further discloses a cell apoptosis detection system based on the micro-fluidic chip. The cell apoptosis detection system comprises an LED (light emitting diode) light source, the micro-fluidic chip and a signal processing system, wherein the signal processing system comprises a photoelectric diode and a phase-locked amplifier; a to-be-detected sample in the micro-fluidic chip emits fluorescent light under the irradiation of the light source; the photoelectric diode collects the fluorescent light emitted by the to-be-detected sample and conveys the fluorescent light to the phase-locked amplifier for analysis. The cell apoptosis detection system is compact in structure and is capable of quickly and quantitatively analyzing cell apoptosis information.

Description

Micro-fluidic chip and the Apoptosis detection system based on this chip
Technical field
The utility model belongs to microbiology detection system field, relates to Apoptosis detection system, particularly a kind of Apoptosis detection system based on micro-fluidic chip.
Background technology
Apoptosis (apoptosis) is the dead form of cell spontaneity under gene regulation, be indispensable significant process in Growth and Differentiation process, generally adopt at present fluorescence microscope to carry out fluorescence qualitative and quantitative analysis to apoptotic cell.When detection, first with fluorescein, cell is dyeed, then under fluorescence microscope, cell is carried out to morphologic observation and quantitative observation.This method behaviour is simple and be convenient to observation directly perceived, is applied by most of researchers.
Also have some not enough but fluorescence microscope detects:
(1) first fluorescence radiant energy consuming ratio is larger, and no matter mercury lamp or halogen lamp, has larger power consumption, affects to a certain extent observing effect, and intensive ultraviolet is harmful to glasses.
(2) fluorescence light source has certain life-span and light source comparatively expensive, needs periodic replacement, and therefore maintenance cost is relatively high.
(3), although use microscope to have morphologic detection advantage intuitively, carry out quantitatively detecting and carry out counting and can relatively waste time and energy.
Summary of the invention
In view of this, the purpose of this utility model is to provide a kind of new microfluidic chip and a kind of Apoptosis detection system based on this micro-fluidic chip.
For achieving the above object, the utility model provides following technical scheme: micro-fluidic chip, and comprise nutrient solution passage and detect liquid passage, described nutrient solution passage is made up of nutrient solution sample holes, nutrient solution runner and detection hole; Described detection liquid passage by detecting liquid sample holes, detect liquid runner and mix aperture and form, described mix aperture with detect hole one by one corresponding and respectively with detect liquid runner and detection hole communicates; Nutrient solution and detection reagent meet and mix in mix aperture.
Further, described detection liquid runner is through the center of mix aperture, and described mix aperture overlaps with detection hole site.
Further, described nutrient solution cross section of fluid channel diameter is 15-40 μ m, and described mix aperture and detection bore dia are 50-150 μ m.
A kind of Apoptosis detection system, comprises LED light source, micro-fluidic chip and signal processing system, and described signal processing system comprises photodiode and lock-in amplifier; Testing sample in micro-fluidic chip sends fluorescence under light source irradiates, and the fluorescence that photodiode collection testing sample sends is also sent to lock-in amplifier analysis.
Further, between described LED light source and micro-fluidic chip, be provided with bandpass filter, between described LED light source and photodiode, be provided with interferometric filter.
Further, described LED light source comprises driving power, light source fixing frame, light source rotating disk and LED electroluminescent lamp; Described light source rotating disk is connected on light source fixing frame and can pivots, and described LED electroluminescent lamp is arranged on light source rotating disk and with driving power and is communicated with.
Further, described bandpass filter, micro-fluidic chip and interferometric filter are combined closely and are fixed on PDMS substrate, and described photodiode arranges between interferometric filter and PDMS substrate.
The beneficial effects of the utility model are: microfluidic chip structure of the present utility model is simply compact, can realize multiple samples detects simultaneously, nutrient solution channel size and the cell size of micro-fluidic chip of the present utility model are suitable, can realize the detection of individual cells, avoid multiple cells to influence each other.Apoptosis detection system of the present utility model is using LED lamp as light source, taking micro-fluidic chip as detecting carrier, records signal with signal processing system analysis, have compact conformation, energy consumption is low, uses safety, high specificity, and can realize fast the quantitative analysis to Apoptosis information.
Brief description of the drawings
In order to make the purpose of this utility model, technical scheme and beneficial effect clearer, the utility model provides following accompanying drawing to describe:
Fig. 1 is the nutrient solution passage plan structure schematic diagram of the utility model micro-fluidic chip;
Fig. 2 is the detection liquid passage plan structure schematic diagram of the utility model micro-fluidic chip;
Fig. 3 is the plan structure schematic diagram of the utility model micro-fluidic chip;
Fig. 4 is the structural representation of the utility model Apoptosis detection system;
Wherein: 1-nutrient solution sample holes, 2-nutrient solution runner, 3-detection hole, 4-detection liquid sample holes, 5-detection liquid runner, 6-mix aperture, 7-light source fixing frame, 8-light source rotating disk, 9-LED electroluminescent lamp, 10-lock-in amplifier, 11-bandpass filter, 12-micro-fluidic chip, 13-interferometric filter, 14-photodiode, 15-PDMS substrate, 16-driving power.
Detailed description of the invention
Below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described in detail.
As shown in Figure 1,2 and 3, micro-fluidic chip of the present utility model, comprises nutrient solution passage and detects liquid passage, described nutrient solution passage is made up of nutrient solution sample holes 1, nutrient solution runner 2 and detection hole 3; Described detection liquid passage by detecting liquid sample holes 4, detect liquid runner 5 and mix aperture 6 and form, described mix aperture 6 with detect hole 3 one by one corresponding and respectively with detect liquid runner 5 and detection hole 3 communicates; Nutrient solution and detection reagent meet and mix in mix aperture 6.
As the improvement of the present embodiment, described detection liquid runner 5 is through the center of mix aperture 6, and described mix aperture 6 overlaps with detection 3 positions, hole.
As further improvement of this embodiment, described nutrient solution runner 2 diameter of sections are 15-40 μ m, and described mix aperture 6 and detection hole 3 diameters are 50-150 μ m.
When use, nutrient solution enters micro-fluidic chip from sample holes 1, then enters and detects hole 3 along nutrient solution runner 2, then flows into mix aperture 6; Detect liquid from sample holes 4 after testing liquid runner 5 enter mix aperture 6; Nutrient solution and detection liquid mix in mix aperture 6, after mixing, can detect.
Microfluidic chip structure of the present utility model is simply compact, can realize multiple samples and detect simultaneously, and nutrient solution channel size and the cell size of micro-fluidic chip of the present utility model are suitable, can realize the detection of individual cells, avoid multiple cells to influence each other.
As shown in Figure 4, the Apoptosis detection system of the present embodiment based on described micro-fluidic chip, comprises LED light source, micro-fluidic chip 12 and signal processing system, and described signal processing system comprises photodiode 14 and lock-in amplifier 10; Testing sample in micro-fluidic chip 12 sends fluorescence under light source irradiates, and photodiode 14 is collected the fluorescence that testing sample sends and is sent to lock-in amplifier 10 and analyzes.
One as the present embodiment is improved, and between described LED light source and micro-fluidic chip 12, is provided with bandpass filter 11, between described LED light source and photodiode 14, is provided with interferometric filter 13.
Another kind as the present embodiment improves, and described LED light source comprises driving power 16, light source fixing frame 7, light source rotating disk 8 and LED electroluminescent lamp 9; Described light source rotating disk 8 is connected on light source fixing frame 7 and can pivots, and described LED electroluminescent lamp 9 is arranged on light source rotating disk 8 and with driving power 16 and is communicated with.
As further improvement of this embodiment, described bandpass filter 11, micro-fluidic chip 12 and interferometric filter 13 are combined closely and are fixed on PDMS substrate 15, and described photodiode 14 arranges between interferometric filter 13 and PDMS substrate.
When use, first assemble above-mentioned parts, then just testing sample is inserted micro-fluidic chip, then open LED electroluminescent lamp, light irradiates micro-fluidic chip through bandpass filter 11, micro-fluidic chip sends fluorescence under light-illuminating, and the signal of telecommunication is collected and be converted into fluorescence by photodiode 14 after interferometric filter 13, and the signal of telecommunication is sent to lock-in amplifier 10 and analyzes.
Apoptosis detection system of the present utility model is using LED lamp as light source, taking micro-fluidic chip as detecting carrier, records signal with signal processing system analysis, have compact conformation, energy consumption is low, uses safety, high specificity, and can realize fast the quantitative analysis to Apoptosis information.
Finally explanation is, above preferred embodiment is only unrestricted in order to the technical solution of the utility model to be described, although the utility model is described in detail by above preferred embodiment, but those skilled in the art are to be understood that, can make various changes to it in the form and details, and not depart from the utility model claims limited range.

Claims (1)

1. micro-fluidic chip, comprises nutrient solution passage and detects liquid passage, it is characterized in that: described nutrient solution passage is by nutrient solution sample holes (1), nutrient solution runner (2) and detect hole (3) formation; Described detection liquid passage by detect liquid sample holes (4), detect liquid runner (5) and mix aperture (6) form, described mix aperture (6) with detect hole (3) one by one corresponding and respectively with detection liquid runner (5) and detection hole (3) communicate; Nutrient solution and detection reagent meet and mix in mix aperture (6).
2. according to micro-fluidic chip described in claim 1, it is characterized in that: described detection liquid runner (5) is through the center of mix aperture (6), and described mix aperture (6) overlaps with detection position, hole (3).
3. according to micro-fluidic chip described in claim 1, it is characterized in that: described nutrient solution runner (2) diameter of section is 15-40 μ m, described mix aperture (6) and detection hole (3) diameter are 50-150 μ m.
4. the Apoptosis detection system based on micro-fluidic chip as described in claim 1-3 any one, it is characterized in that: comprise LED light source, micro-fluidic chip (12) and signal processing system, described signal processing system comprises photodiode (14) and lock-in amplifier (10); Testing sample in micro-fluidic chip (12) sends fluorescence under light source irradiates, and photodiode (14) is collected the fluorescence that sends of testing sample and is sent to lock-in amplifier (10) and analyzes.
5. according to the Apoptosis detection system of micro-fluidic chip described in claim 4, it is characterized in that: between described LED light source and micro-fluidic chip (12), be provided with bandpass filter (11), between described LED light source and photodiode (14), be provided with interferometric filter (13).
6. according to the Apoptosis detection system of micro-fluidic chip described in claim 4 or 5, it is characterized in that: described LED light source comprises driving power (16), light source fixing frame (7), light source rotating disk (8) and LED electroluminescent lamp (9); Described light source rotating disk (8) is connected to light source fixing frame (7) above and can pivots, and described LED electroluminescent lamp (9) is arranged on light source rotating disk (8) above and is communicated with driving power (16).
7. according to the Apoptosis detection system of micro-fluidic chip described in claim 6, it is characterized in that: described bandpass filter (11), micro-fluidic chip (12) and interferometric filter (13) are combined closely and be fixed on PDMS substrate, and described photodiode (14) arranges between interferometric filter (13) and PDMS substrate.
CN201320877797.4U 2013-12-27 2013-12-27 Micro-fluidic chip and cell apoptosis detection system based on same Expired - Lifetime CN203778087U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104212706A (en) * 2014-09-19 2014-12-17 成都劲宏科技有限公司 Lock-in amplification technology based cell microflow image acquisition system
CN105928893A (en) * 2016-05-23 2016-09-07 杭州霆科生物科技有限公司 Multi-sample multi-index micro-fluidic optical detection system
CN108152249A (en) * 2017-12-22 2018-06-12 太原理工大学 Detect the optical biosensor and method of DNA mismatch in free fluid

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104212706A (en) * 2014-09-19 2014-12-17 成都劲宏科技有限公司 Lock-in amplification technology based cell microflow image acquisition system
CN104212706B (en) * 2014-09-19 2016-03-02 成都劲宏科技有限公司 A kind of cell microfluidic image capturing system based on phase lock amplifying technology
CN105928893A (en) * 2016-05-23 2016-09-07 杭州霆科生物科技有限公司 Multi-sample multi-index micro-fluidic optical detection system
CN108152249A (en) * 2017-12-22 2018-06-12 太原理工大学 Detect the optical biosensor and method of DNA mismatch in free fluid
CN108152249B (en) * 2017-12-22 2021-03-26 太原理工大学 Optical biosensor and method for detecting DNA mismatch in free liquid

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