CN103340309B - Pseudobagrus fulvidraco feed additive capable of improving immune function and application thereof - Google Patents
Pseudobagrus fulvidraco feed additive capable of improving immune function and application thereof Download PDFInfo
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- CN103340309B CN103340309B CN201310273716.4A CN201310273716A CN103340309B CN 103340309 B CN103340309 B CN 103340309B CN 201310273716 A CN201310273716 A CN 201310273716A CN 103340309 B CN103340309 B CN 103340309B
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Abstract
The invention discloses a pseudobagrus fulvidraco feed additive capable of improving an immune function. The pseudobagrus fulvidraco feed additive is characterized by being prepared from 60-85 parts by weight of hericium erinaceus and 45-95 parts of black fungus which are ground, fermented, concentrated, filtered, dried, ground and then are blended with 5-10 parts by weight of xylooligosaccharide. The pseudobagrus fulvidraco feed additive has the effects of enhancing the immunity and an organism function. A feed with the feed additive is conductive to the enhancement of the organism function and the own immunity of pseudobagrus fulvidraco and can be used for enhancing the phagocytic activity and the lysozyme activity of pseudobagrus fulvidraco blood cells. The pseudobagrus fulvidraco feed additive disclosed by the invention has the advantages of wide sources in components, low cost and suitability for application in large-scale cultivation.
Description
Technical field
The present invention relates to a kind of feed addictive and application thereof, specifically a kind of yellow-head catfish feed additive and application thereof improving immunity function.
Background technology
Pelteobagrus fulvidraco (Pelteobagrus fulvidrac) belongs to Silurformes, Yu Wei section, Pelteobagrus.Be distributed in the waters such as China rivers and creeks, lake, irrigation canals and ditches.Due to Pelteobagrus fulvidraco fine and tender taste, meat flavour deliciousness, thus extensively like by consumer, in recent years, become one of domestic emerging freshwater aquiculture kind.Along with developing rapidly of Pelteobagrus fulvidraco intensive culture, its disease is also on the rise, and the immunity function improving Pelteobagrus fulvidraco is significant for aquaculture.
Summary of the invention
Goal of the invention: the object of the present invention is to provide a kind of yellow-head catfish feed additive, to realize the physical function, the raising immunocompetence that are easy to prepare, be convenient to promote, can effectively improve Pelteobagrus fulvidraco.Present invention also offers the application of this feed addictive.
Technical scheme: in order to realize foregoing invention object, the technical solution adopted in the present invention is:
Improve a yellow-head catfish feed additive for immunity function, it is characterized in that: described additive by the Hericium erinaceus of weight portion 60 ~ 85 parts and the black fungus of weight portion 45 ~ 95 parts through pulverizing, fermentation, concentrated, filter, dry, pulverize after mixed with the xylo-oligosaccharide of weight portion 5 ~ 10 parts.
The preparation process of described additive is as follows:
(1) pulverize: choose new fresh Hericium erinaceus and black fungus, dry dehydration, the Hericium erinaceus of weight portion 60 ~ 85 parts and the black fungus grinder of weight portion 45 ~ 95 parts after dehydration are ground, crosses 80 ~ 120 mesh sieves;
(2) ferment: by the powder of step (1) access culture medium; The weight ratio of powder and culture medium is 1 ~ 5:50 ~ 80; Fermentation temperature 26 ~ 28 DEG C, pH value 6 ~ 7, time 0.5 ~ 2h;
(3) preparation of Hericium erinaceus Blackfungus polyhexose: be 0.2 ~ 0.3um membrane filtration by aperture by the zymotic fluid of step (2); Reduced pressure concentration, filtration after filtering; Hericium erinaceus Blackfungus polyhexose is obtained by after filter vacuum drying;
(4) step (3) Hericium erinaceus Blackfungus polyhexose is pulverized; Mixed with the xylo-oligosaccharide of weight portion 5 ~ 10 parts again.
Described culture medium comprises the component of following mass percent: glucose 1.2 ~ 2.8%, tryptone 0.5 ~ 1.8%, yeast leaching powder 1.5 ~ 2.5%, ammonium sulfate 0.05 ~ 0.18%, sodium acid carbonate 0.05 ~ 0.20%, corn starch 0.2 ~ 0.35%.
The vacuum of described reduced pressure concentration is 0.05-0.15MPa, and temperature is 60-80 DEG C.
Described vacuum drying vacuum pressure is 0.10-0.15MPa, and temperature is 60-80 DEG C.
Described vacuum drying is until the weight percentage of moisture is less than or equal to 3%.
Described feed addictive joins in basal feed according to the addition of mass fraction 0.5 ~ 2%.
Beneficial effect: compared with prior art, remarkable advantage of the present invention is: feed addictive of the present invention has raising immunity, strengthens the effect of fuselage function.The feed that with the addition of feed addictive contributes to the body function and the autoimmunity that improve Pelteobagrus fulvidraco, strengthens activate the phagocytic capacity, the antalzyme activity of Pelteobagrus fulvidraco haemocyte.Pelteobagrus fulvidraco ingredient origin of the present invention is extensive, cheap, is applicable to large-scale farming and uses.
Detailed description of the invention
Below by specific embodiment, the present invention is further described; it should be pointed out that for the person of ordinary skill of the art, under the premise without departing from the principles of the invention; can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Embodiment 1
Choose new fresh Hericium erinaceus and black fungus, choose new fresh Hericium erinaceus and black fungus, dry dehydration, the Hericium erinaceus of weight portion 60g and the black fungus grinder of weight portion 45g after dehydration are ground, crosses 80 mesh sieves; Powder is accessed culture medium; The weight ratio of powder and culture medium is 1:50; Culture medium comprises the component of following mass percent: glucose 1.2%, tryptone 0.5%, yeast leaching powder 1.5%, ammonium sulfate 0.05%, sodium acid carbonate 0.05%, corn starch 0.2%.; Fermentation temperature 26 DEG C, pH value 6, time 0.5h; Zymotic fluid is 0.2um membrane filtration by aperture; In vacuum 0.05MPa after filtration, temperature 60 C reduced pressure concentration, filtration; By filtrate in vacuum pressure 0.10MPa, temperature 60 C vacuum drying is until the weight percentage of moisture obtains Hericium erinaceus Blackfungus polyhexose after being less than or equal to 3%; Hericium erinaceus Blackfungus polyhexose is mixed with the xylo-oligosaccharide of weight portion 5g again after pulverizing.Obtained feed addictive is joined in basal feed according to the addition of mass fraction 0.5% and eats for Pelteobagrus fulvidraco.
Embodiment 2
Choose new fresh Hericium erinaceus and black fungus, dry dehydration, the Hericium erinaceus of weight portion 85g and the black fungus grinder of weight portion 95g after dehydration are ground, crosses 120 mesh sieves; Powder access culture medium; Culture medium comprises the component of following mass percent: glucose 2.8%, tryptone 1.8%, yeast leaching powder 2.5%, ammonium sulfate 0.18%, sodium acid carbonate 0.20%, corn starch 0.35%.The weight ratio of powder and culture medium is 5:80; Fermentation temperature 28 DEG C, pH value 7, time 2h; Zymotic fluid is 0.3um membrane filtration by aperture; In vacuum 0.15MPa after filtration, temperature 60-80 DEG C of reduced pressure concentration, filtration; By filtrate in vacuum pressure 0.15MPa, temperature 60-80 DEG C vacuum drying until the weight percentage of moisture obtains Hericium erinaceus Blackfungus polyhexose after being less than or equal to 3%; Hericium erinaceus Blackfungus polyhexose is mixed with the xylo-oligosaccharide of 10g again after pulverizing.Obtained feed addictive is joined in basal feed according to the addition of mass fraction 2% and eats for Pelteobagrus fulvidraco.
Embodiment 3
Choose new fresh Hericium erinaceus and black fungus, dry dehydration, the Hericium erinaceus of weight portion 70 parts and the black fungus grinder of weight portion 65 parts after dehydration are ground, crosses 100 mesh sieves; Powder access culture medium; Culture medium comprises the component of following mass percent: glucose 2%, tryptone 1.2%, yeast leaching powder 2%, ammonium sulfate 0.12%, sodium acid carbonate 0.15%, corn starch 0.30%.The weight ratio of powder and culture medium is 3:65; Fermentation temperature 27 DEG C, pH value 6.5, time 1h; Zymotic fluid is 0.25um membrane filtration by aperture; In vacuum 0.10MPa after filtration, temperature 70 C reduced pressure concentration, filtration; By filtrate in vacuum pressure 0.12MPa, temperature 70 C vacuum drying is until the weight percentage of moisture obtains Hericium erinaceus Blackfungus polyhexose after being less than or equal to 3%; Hericium erinaceus Blackfungus polyhexose is mixed with the xylo-oligosaccharide of 8g again after pulverizing.Obtained feed addictive is joined in basal feed according to the addition of mass fraction 1% and eats for Pelteobagrus fulvidraco.
Embodiment 4
The present embodiment chooses healthy Pelteobagrus fulvidraco 540 tail in 2 ages from cultivation fishing ground, average weight (61.6 ± 5.4) g, long (19.5 ± 3.2) cm of average body, is divided into 6 groups at random, often organize 3 repetitions, each repetition 30 tail fish (male and female respectively account for 1/2).6 groups of test fishes feed respectively to add in basal feed 0 (control group), 300,600,900,1200, the test feed of 1500mg/kg feed addictive of the present invention.
After Pelteobagrus fulvidraco cultivates 8 weeks, attack malicious 96h through Edwardsiella tarda viable bacteria, result of the test is in table 1; the control group death rate is 80%, and the death rate of each interpolation group all decreases, and obtains certain immune protective rate; especially 1500mg/kg group, the death rate is only 40%, and immune protective rate reaches 60%.In addition, in test there is certain dose relationship in the death rate of Pelteobagrus fulvidraco and immune protective rate and feed addictive Pitch-based sphere.
Table 1
| Group | Attack malicious fish quantity (tail) | Dead fish quantity (tail) | The death rate (%) | Immune protective rate (%) |
| 0mg/kg | 20 | 17 | 85 | / |
| 300mg/kg | 20 | 13 | 65 | 22 |
| 600mg/kg | 20 | 12 | 60 | 25 |
| 900mg/kg | 20 | 10 | 50 | 37.5 |
| 1200mg/kg | 20 | 9 | 45 | 50 |
| 1500mg/kg | 20 | 8 | 40 | 60 |
Embodiment 5
The present embodiment selects neat specification, physically fit, and (Pelteobagrus fulvidraco 240 tail of 10.39 ± 0.68g, random point 4 groups, often group establishes 3 repetitions to average body quality, every repeating groups 20 tail fish.I ~ III group is test group, and the feed of the feed addictive that to the addition of 0.5%, 1.0%, 2.0% in basal feed of feeding respectively, separately establishes a control group, basal feed of throwing something and feeding.Test is carried out 60d and is stopped feeding, and stop eating 2d, measure activate the phagocytic capacity, the antalzyme activity of Pelteobagrus fulvidraco haemocyte.
Immune indexes measures:
1, take a blood sample
Stop raising rear 2d, fish for 10 tails at random for examination fish, anaesthetize fish body with MS-222 (150mg/L), the syringe tail veins blood sampling that 1% heparin is moistening.The blood of every tail fish is divided into 2 parts, and wherein 1 part of blood first at room temperature leaves standstill 1h and treats that it solidifies, and puts 4 DEG C, refrigerator and preserves 4h, 4 DEG C, the centrifugal 10min of 4000r/min, collect serum and be used for lysozyme assay; Another 1 part with anticoagulant heparin, prepare anticoagulation, active for mensuration leukocytes phagocytic.
2, the mensuration of phagocyte phagocytic function
In anticoagulation, add 0.25ml staphylococcus aureus suspension, fully after mixing at 28 DEG C water-bath 1h, shake up 1 time every 10min during water-bath.Add the centrifugal 4min of 0.15mol/L cold NaCl solution 0.8ml, 2000r/min again, abandoning supernatant.Then draw haemocyte smear with suction pipe, each blood sample is coated with 5, and methyl alcohol fixes 5 ~ 7min, distilled water flushing, Giemsa dye 1 ~ 5h, washes and dries rear microscopy.Activate the phagocytic capacity represents with percentage phagocytosis (PP) and phagocytic index (PI) respectively: PP%=(participating in cell number/100 of engulfing in 100 phagocytes) × 100%; PI%=(the phagocyte number that the total number of bacteria/participation in phagocyte is engulfed) × 100%.
3, the mensuration of lysozyme (LSZ) vigor
Take micrococcus lysodeikticus as substrate, with 0.1mol/ml phosphate buffer (pH6.4), micrococcus lysodeikticus is made into the bacteria suspension of 0.3mg/ml, 3ml bacteria suspension got by each sample, drawing 0.04ml serum adds in bacteria suspension, measures AO value immediately after mixing with UV-2000 spectrophotometer under 570nm.Then after temperature bath 30min, take out and be placed in 4 DEG C of water-bath 10min with cessation reaction, measure it in the OD value A at 570nm place, be calculated as follows antalzyme activity: U=(AO-A)/AO in 37 DEG C of water-baths.
Measurement result is in table 2.Result shows, the feed containing 0.5%, 1.0%, 2.0% feed addictive all can improve the phagocytic activity of Pelteobagrus fulvidraco significantly, and its percentage phagocytosis, phagocytic index are compared with control group, and difference is all extremely remarkable, and Pelteobagrus fulvidraco serum lysozyme level also improves significantly.1.0% feed addictive group percentage phagocytosis, phagocytic index are better than 0.5% feed addictive group and 2.0% feed addictive group.
Table 2
| Group | PP% | PI% | Lysozyme activity |
| Control group | 44.38±3.68 | 4.27±0.29 | 2.248±0.015 |
| 0.5% interpolation group | 57.38±2.69 | 5.09±0.68 | 0.368±0.021 |
| 1% interpolation group | 66.25±3.46 | 6.17±1.29 | 0.421±0.012 |
| 2% interpolation group | 64.27±2.32 | 6.02±0.98 | 0.427±0.031 |
Claims (5)
1. improve a yellow-head catfish feed additive for immunity function, it is characterized in that: described additive by the Hericium erinaceus of weight portion 60 ~ 85 parts and the black fungus of weight portion 45 ~ 95 parts through pulverizing, fermentation, concentrated, filter, dry, pulverize after mixed with the xylo-oligosaccharide of weight portion 5 ~ 10 parts.
2. the yellow-head catfish feed additive improving immunity function as claimed in claim 1, is characterized in that: the preparation process of described additive is as follows:
(1) pulverize: choose new fresh Hericium erinaceus and black fungus, dry dehydration, by dewatering, the Hericium erinaceus of weight portion 60 ~ 85 parts and the black fungus grinder of weight portion 45 ~ 95 parts afterwards grind, and cross 80 ~ 120 mesh sieves;
(2) ferment: by the powder of step (1) access culture medium; The weight ratio of powder and culture medium is 1 ~ 5:50 ~ 80; Fermentation temperature 26 ~ 28 DEG C, pH value 6 ~ 7, time 0.5 ~ 2h;
(3) preparation of Hericium erinaceus Blackfungus polyhexose: be 0.2 ~ 0.3um membrane filtration by aperture by the zymotic fluid of step (2); Reduced pressure concentration, filtration after filtering; Hericium erinaceus Blackfungus polyhexose is obtained by after filter vacuum drying;
(4) step (3) Hericium erinaceus Blackfungus polyhexose is pulverized; Mixed with the xylo-oligosaccharide of weight portion 5 ~ 10 parts again.
3. the yellow-head catfish feed additive improving immunity function as claimed in claim 2, it is characterized in that: described culture medium comprises the component of following mass percent: glucose 1.2 ~ 2.8%, tryptone 0.5 ~ 1.8%, yeast leaching powder 1.5 ~ 2.5%, ammonium sulfate 0.05 ~ 0.18%, sodium acid carbonate 0.05 ~ 0.20%, corn starch 0.2 ~ 0.35%.
4. the yellow-head catfish feed additive improving immunity function as claimed in claim 2, is characterized in that: described vacuum drying is until the weight percentage of moisture is less than or equal to 3%.
5. the application of the yellow-head catfish feed additive of raising immunity function according to claim 1: it is characterized in that: described feed addictive joins in basal feed according to the addition of mass fraction 0.5 ~ 2%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103494046A (en) * | 2013-10-27 | 2014-01-08 | 黑龙江省轻工科学研究院 | Preparation method of feed additives for pelteobagrus fulvidraco |
| CN106071376A (en) * | 2016-06-17 | 2016-11-09 | 安庆市皖宜季牛水产养殖有限责任公司 | A kind of high protein feed promoting Pelteobagrus fulvidraco growth of seedling |
| CN108157678A (en) * | 2017-12-29 | 2018-06-15 | 磐安县派普特生物科技有限公司 | A kind of preparation method containing Auricularia polysaccharide preparing grass carp feed additive |
| CN109275804A (en) * | 2018-11-07 | 2019-01-29 | 宁德师范学院 | It is a kind of to improve the feed and preparation method thereof that Larimichthys crocea enteron aisle is digested and assimilated |
| CN110292104A (en) * | 2019-08-02 | 2019-10-01 | 江门海大饲料有限公司 | A kind of Pelteobagrus fulvidraco mixed feed of megathermal period and its preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101326964A (en) * | 2008-07-22 | 2008-12-24 | 浙江大学 | Feedstuff additive for reinforcing immunity of sea water fish |
| CN102599330A (en) * | 2012-03-28 | 2012-07-25 | 常熟市汇康食品厂 | Auricularia bran |
-
2013
- 2013-06-28 CN CN201310273716.4A patent/CN103340309B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101326964A (en) * | 2008-07-22 | 2008-12-24 | 浙江大学 | Feedstuff additive for reinforcing immunity of sea water fish |
| CN102599330A (en) * | 2012-03-28 | 2012-07-25 | 常熟市汇康食品厂 | Auricularia bran |
Non-Patent Citations (3)
| Title |
|---|
| 低聚木糖在水生动物生产中的应用研究进展;徐善金等;《饲料广角》;20111231(第22期);第41-43页 * |
| 猴头菇菌丝体和发酵液中多糖的含量测定;刘晓明等;《中国医药科学》;20130331;第3卷(第6期);第94-95页 * |
| 黑木耳多糖及其生物活性;张大为等;《食品与药品》;20071231;第9卷(第9期);第68-70页 * |
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