CN103333790B - A kind of Novel aseptic plate medium and preparation method thereof - Google Patents
A kind of Novel aseptic plate medium and preparation method thereof Download PDFInfo
- Publication number
- CN103333790B CN103333790B CN201310281905.6A CN201310281905A CN103333790B CN 103333790 B CN103333790 B CN 103333790B CN 201310281905 A CN201310281905 A CN 201310281905A CN 103333790 B CN103333790 B CN 103333790B
- Authority
- CN
- China
- Prior art keywords
- ware
- upper cover
- groove
- setting adhesive
- substratum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides a kind of Novel aseptic plate medium and preparation method thereof, comprise following step: steps A: lower surface non-setting adhesive being printed on upper plastic cover; Step B: by ware under injection moulding machine manufacture Novel culture dish; Step C: the liquid nutrient agar prepared is poured in described lower ware groove, train line is treated it is laid in bottom portion of groove and cooled and solidified; Step D: after nutrient agar solidifies, the upper cover plastic film being printed on non-setting adhesive is impressed on lower ware by train line, ensures the correct position that non-setting adhesive is connected with lower ware epitaxial part, ensures the sealing of culture dish; Step e: folded by packing specifications by the sterilized petri dishes substratum of good seal, through gamma sterilization after packaging vanning.In the present invention, due to the design of Novel aseptic plate medium, without the need to manually adding a cover, thus reducing substratum open-assembly time, continuous scale operation can be realized.Breach and often criticize prefabricated sterilized petri dishes substratum and criticize quantitative limitation, greatly can reduce the production cost of prefabricated sterilized petri dishes substratum.
Description
Technical field
The present invention relates to a kind of Novel aseptic plate medium and preparation method thereof.
Background technology
Sterilized petri dishes substratum is cultivated as a kind of traditional microbiological, detection reagent uses very general in pharmaceutical manufacturing, scientific research, such as gnotobasis monitoring, microorganism culturing qualification etc.
Traditional self-control sterilized petri dishes substratum generally pours 50-60 DEG C of liquid nutrient agar in an aseptic environment with the 90mm diameter Petri dishes through sterilizing, seals and make after its cooling.Shortcoming is full artificially, length consuming time, batch is little, stability is not high, storage time is short.
Current commercialization prefabricated sterilized petri dishes substratum is same use aforesaid method preparation generally, then make with irradiation sterilization after the sealing of three-layer plastic bag, although compare self-made medium to increase in every respect, but still need manually to add a cover or add a cover with machine, production lot is low, artificial and hardware cost is higher, the Shortcomings of product structure own.
At present, no matter at testing laboratory or all kinds of production plant of R&D institution, school, hospital, pharmaceutical manufacturing enterprise, the consumption of the sterilized petri dishes substratum that Micro biological Tests uses is very huge, according to statistics, production cycle of general Biopharmaceutical Enterprises or workshop monthly regularly environmental monitoring once just need about 100 plating medium.Use for laboratory substratum generally has two kinds of sources.
The first source is, user aseptically oneself preparation substratum, but this method not only operation is loaded down with trivial details, quantity is few, also very consuming time, be not enough to cope with contingencies, need fast, and exist larger people be microbiological contamination risk.
Sterilized petri dishes substratum is bought in the second source outside, this method is time saving and energy saving, but the validity period of the sterilized petri dishes substratum of market generally only has 3 months at present, and most of brand does not have independent sealed packaging, user just must be finished the substratum of a packing unit at short notice once Kaifeng, can not correspond to actual needs.Product structure aspect, mostly adopts the embedding conventional combination mode being placed on chassis of cover cap, brings certain risk to the gnotobasis in sterilized petri dishes substratum.Face on a manufacturing scale, production efficiency is limited by the structure of product, and according to investigations, the producer's batch producing sterilized petri dishes substratum on the market is greatly limited to less than 5000, can not deal with the heavy demand of emergency event.
Summary of the invention
The object of the invention is, invent a kind of sterile plating medium structure, design the equipment and process that forms a complete production network, the prefabricated sterilized petri dishes substratum of extensive preparation.
A kind of Novel aseptic plate medium, is characterized in that, comprising: upper cover, the lower ware matched with upper cover, the epitaxial part that lower ware comprises groove and is connected with groove, the upper cover part contacted with epitaxial part scribbles non-setting adhesive.
Preferably, described groove is the cylinder shape groove of diameter 90mm, and thickness is 0.6mm.
Preferably, the ware of Novel aseptic plate medium adopts plastic material.
Preferably, described epitaxial part is a plane square, and length and width is 110mm, thickness 0.6mm, and four angles are cut to arc-shaped, and the edge at described four angles is mellow and full shape.
Preferably, described sunk part height is 15mm.
Present invention also offers a kind of method preparing novel plating medium, comprise following step:
Steps A: lower surface non-setting adhesive being printed on upper plastic cover;
Step B: by ware under injection moulding machine manufacture Novel culture dish;
Step C: the liquid nutrient agar prepared is poured in described lower ware groove, train line is treated it is laid in bottom portion of groove and cooled and solidified;
Step D: after nutrient agar solidifies, the upper cover plastic film being printed on non-setting adhesive is impressed on lower ware by train line, ensures the correct position that non-setting adhesive is connected with lower ware epitaxial part, ensures the sealing of culture dish;
Step e: folded by packing specifications by the sterilized petri dishes substratum of good seal, through gamma sterilization after packaging vanning.
Preferably, the wide of roll of plastic of described steps A is 110mm, and thick is 0.2mm.
Preferably, in described step D, the temperature of described liquid nutrient agar is 50 ~ 60 DEG C of insulations, loads in described groove with the volume of 15 ~ 20ml/ ware.
Preferably, in described step e, packing specifications is 10 wares/bag.
The present invention adopts above technical scheme, and beneficial effect is.
1. sterile plating medium is simple for structure, easy to use, achieves massive mechanical continuous seepage simultaneously.
2. each sterilized petri dishes substratum is independent sealed, is convenient to adjust usage quantity flexibly.
3. the form construction design of uniqueness is convenient to user's mark and is taken.
For the above, applicant makes following novelty by research and development and improves.
1, independent sealed.Adopt the mode of chassis independent sealer after filling cooling, both ensured the self-contained sterile of each plating medium, and greatly reduced again the risk of microbiological contamination in culturing process, use flexibly.
2, produce in enormous quantities.The method that the traditional staff of the process free of mechanize sealer is added a cover, achieves production in enormous quantities while reducing the microbiological contamination risk in production process, greatly reduces production cost, batch turnout reaches 50,000/batch more than.
3, easy to use.The epitaxial part of the lower cover of sterilized petri dishes substratum is square, and four angles are arc-shaped, and edge polishing is mellow and full does not cut hand, and corner extension is used as experimental record, avoids record vestige to the interference of experimental result.
4, shelf lives is long.Substratum is dispensed in lower cover by machine after joining filter, mechanize sealer, packing after subcooling, and then irradiation sterilization is carried out in unification.This product preservation period under 2 DEG C ~ 25 DEG C conditions reaches 1 year, still keeps sterility and the growth promotion to bacterium colony in preservation period.
5, suitability is wide.This production technique can be applied on ten many kinds of solids substratum, as nutrient agar, TSA substratum, Sabouraud glucose agar, MH nutrient agar etc., application aspect covers the multiple-microorganisms such as escherichia coli, Pseudomonas aeruginosa, streptococcus aureus, subtilis, aspergillus niger, streptococcus albus, yeast and tests long inspection project bacterial classification.
Relative to prior art, due to the design of Novel aseptic plate medium in the present invention, without the need to manually adding a cover, reducing substratum open-assembly time, continuous scale operation can be realized.Breach and often criticize prefabricated sterilized petri dishes substratum and criticize quantitative limitation, greatly can reduce the production cost of prefabricated sterilized petri dishes substratum.
Accompanying drawing explanation
Fig. 1 is ware vertical view under Novel aseptic plate medium of the present invention.
Fig. 2 is ware side-view under Novel aseptic plate medium of the present invention.
Fig. 3 is Novel aseptic plate medium upper cover side of the present invention upward view.
Fig. 4 is that Novel aseptic plate medium of the present invention adds a cover side view cutaway drawing.
Embodiment
Below in conjunction with accompanying drawing, preferably embodiment of the present invention is described in further detail:
Face is the technological process of production.
Embodiment 1, as shown in Figure 1 and Figure 4.
1, culture dish is plastics, and thickness is 0.6mm.
2, sunk part 20 is the cylinder shape groove of diameter 90mm.
3, epitaxial part 10 is a plane square, and length and width is 110mm, thickness 0.6mm, and four angles are cut to arc-shaped, and edge polishing is mellow and full does not cut hand.
4, epitaxial part 10 is double-sided adhesive position 11, double-sided adhesive is printed on upper cover 30 lower surface, this position can be positioned after lower ware and upper cover 30 seal, and the adhesive power of non-setting adhesive and lower ware is greater than the adhesive power of non-setting adhesive and upper cover 30, non-setting adhesive of again uncapping will adhere to lower ware extension, be separated with upper cover 30; Internal diameter is 96mm, and external diameter is 110mm, thickness 0.1mm.
Embodiment 2, as shown in Figure 2:
Sunk part 20 is highly 15mm, and epitaxial part 10 thickness is 0.6mm.
Embodiment 3, as shown in Figure 3 and Figure 4:
Culture dish upper cover 30 adopts plane square, and length and width is 110mm, thickness 0.2mm, and four angles are cut to arc-shaped, and edge polishing is mellow and full does not cut hand.
Double-sided adhesive is printed on upper cover 30 lower surface, and the adhesive power of non-setting adhesive and lower ware is greater than non-setting adhesive in the adhesive power of upper cover 30, and non-setting adhesive of again uncapping will adhere to lower ware extension, be separated with upper cover 30; Internal diameter is 96mm, and external diameter is 110mm, and thickness is 0.1mm.
Embodiment 4:
As shown in Figure 4, the thickness of culture dish upper cover 30 is 0.2mm; The thickness of double-sided adhesive is 0.1mm; Epitaxial part 10 thickness is 0.6mm; The height of sunk part 20 is 15mm, thickness is 0.6mm.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (3)
1. prepare the method for Novel aseptic plate medium for one kind, it is characterized in that, described Novel aseptic plate medium comprises: upper cover, the lower ware matched with upper cover, the epitaxial part that described lower ware comprises groove and is connected with described groove, described epitaxial part is connected with described upper cover by non-setting adhesive;
Described method comprises following step:
Steps A: lower surface non-setting adhesive being printed on upper plastic cover;
Step B: by ware under injection moulding machine manufacture Novel culture dish;
Step C: the liquid nutrient agar prepared is poured in described lower ware groove, train line is treated it is laid in bottom portion of groove and cooled and solidified;
Step D: after nutrient agar solidifies, the upper cover plastic film being printed on non-setting adhesive is impressed on lower ware by train line, ensures the correct position that non-setting adhesive is connected with lower ware epitaxial part, ensures the sealing of culture dish;
Step e: folded by packing specifications by the sterilized petri dishes substratum of good seal, through gamma sterilization after packaging vanning;
The wide of roll of plastic of described steps A is 110mm, and thick is 0.2mm;
In described step D, described liquid asepsis nutrient agar, with 50 ~ 60 DEG C of insulations, loads in described groove with the volume of 15 ~ 20ml/ ware;
Described groove is the cylinder shape groove of diameter 90mm, and thickness is 0.6mm;
Described epitaxial part is plane square, and length and width is 110mm, thickness 0.6mm, and four angles are cut to arc-shaped, and the edge at described four angles is mellow and full shape;
The height of described groove is 15mm;
Culture dish is plastics, and thickness is 0.6mm;
Epitaxial part is double-sided adhesive position, double-sided adhesive is printed on upper cover lower surface, is positioned this position after lower ware and upper cover seal, and the adhesive power of non-setting adhesive and lower ware is greater than the adhesive power of non-setting adhesive and upper cover, non-setting adhesive of again uncapping will adhere to lower ware extension, be separated with upper cover.
2. prepare the method for Novel aseptic plate medium as claimed in claim 1, it is characterized in that, described lower ware adopts plastic material.
3. prepare the method for Novel aseptic plate medium as claimed in claim 1, it is characterized in that, in described step e, employing packing specifications is 10 wares/bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310281905.6A CN103333790B (en) | 2013-07-05 | 2013-07-05 | A kind of Novel aseptic plate medium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310281905.6A CN103333790B (en) | 2013-07-05 | 2013-07-05 | A kind of Novel aseptic plate medium and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103333790A CN103333790A (en) | 2013-10-02 |
| CN103333790B true CN103333790B (en) | 2016-01-20 |
Family
ID=49241995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310281905.6A Active CN103333790B (en) | 2013-07-05 | 2013-07-05 | A kind of Novel aseptic plate medium and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103333790B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937663A (en) * | 2014-05-08 | 2014-07-23 | 杭州北望生物技术有限公司 | Sterile culture medium plate for environment monitoring of antibiotic production workshop and preparation method of sterile culture medium plate |
| CN110607243A (en) * | 2019-10-22 | 2019-12-24 | 中秀科技股份有限公司 | Culture medium for separating fungi and yeast-like fungi and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4280002A (en) * | 1979-12-03 | 1981-07-21 | Minnesota Mining And Manufacturing Company | Microbiological growth container |
| DE3218532A1 (en) * | 1982-05-17 | 1983-11-17 | C.A. Greiner und Söhne GmbH & Co KG, 7440 Nürtingen | Dish containing a nutrient medium for the cultivation of bacteria and small fungi |
| CN2031795U (en) * | 1988-04-26 | 1989-02-01 | 天津市第二医学院 | Thin-type plastic culture utensil |
| CN1083106A (en) * | 1992-08-25 | 1994-03-02 | 李建秋 | Disposable culture dish for anaerobe |
| CN102272286A (en) * | 2009-01-12 | 2011-12-07 | 赛多利斯史泰迪生物技术有限责任公司 | Transfer unit and method for receiving a medium from a processing device |
| CN202290077U (en) * | 2011-10-30 | 2012-07-04 | 周长波 | Magnetic culture dish box |
| CN202766533U (en) * | 2012-09-11 | 2013-03-06 | 青岛德瑞骏发生物科技有限公司 | Culture vessel |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978103B (en) * | 2012-03-06 | 2014-10-22 | 牛刚 | Manufacturing apparatus and manufacturing method for small sterile prefabricated culture dish |
-
2013
- 2013-07-05 CN CN201310281905.6A patent/CN103333790B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4280002A (en) * | 1979-12-03 | 1981-07-21 | Minnesota Mining And Manufacturing Company | Microbiological growth container |
| DE3218532A1 (en) * | 1982-05-17 | 1983-11-17 | C.A. Greiner und Söhne GmbH & Co KG, 7440 Nürtingen | Dish containing a nutrient medium for the cultivation of bacteria and small fungi |
| CN2031795U (en) * | 1988-04-26 | 1989-02-01 | 天津市第二医学院 | Thin-type plastic culture utensil |
| CN1083106A (en) * | 1992-08-25 | 1994-03-02 | 李建秋 | Disposable culture dish for anaerobe |
| CN102272286A (en) * | 2009-01-12 | 2011-12-07 | 赛多利斯史泰迪生物技术有限责任公司 | Transfer unit and method for receiving a medium from a processing device |
| CN202290077U (en) * | 2011-10-30 | 2012-07-04 | 周长波 | Magnetic culture dish box |
| CN202766533U (en) * | 2012-09-11 | 2013-03-06 | 青岛德瑞骏发生物科技有限公司 | Culture vessel |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103333790A (en) | 2013-10-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103333790B (en) | A kind of Novel aseptic plate medium and preparation method thereof | |
| CN211056684U (en) | Bovine serum filling equipment | |
| BR112022006798A2 (en) | LARGE SCALE SOLID STATE FERMENTATION PROCESS AND PRODUCTION SYSTEM | |
| CN203440354U (en) | Novel sterile plate culture medium | |
| CN204589172U (en) | A kind of active type culture dish | |
| CN103937663A (en) | Sterile culture medium plate for environment monitoring of antibiotic production workshop and preparation method of sterile culture medium plate | |
| CN203715637U (en) | Concentration gradient detection kit for bacterial/fungus drug sensitivity test | |
| CN203625365U (en) | Sealed culture dish | |
| CN105219634A (en) | Microbial rapid detection many ring tests sheet, aseptic culture medium and preparation method thereof | |
| CN202754992U (en) | Novel microorganism culture dish | |
| CN203346379U (en) | Simple bacterial biofilm generation device | |
| CN202643695U (en) | Culture dish with label | |
| CN204848845U (en) | Microorganism short -term test multiple ring test piece | |
| CN1986350A (en) | Live cell posting method | |
| CN205556658U (en) | Bacterium filter cartridge | |
| CN203889276U (en) | Packaging device for aseptic microbiological culture medium | |
| CN102816827A (en) | Preparation method for culture medium product for medicine industry environment microorganism monitoring | |
| CN204434631U (en) | A kind of structure of cell cultures board clamp | |
| CN205443268U (en) | Culture medium storage device | |
| CN205035393U (en) | Cell co -culture orifice plate box | |
| CN213895829U (en) | Culture medium culture device | |
| CN202898433U (en) | Culture medium product for monitoring microbial environment | |
| CN204342795U (en) | A kind of microbial culture dish | |
| CN203346380U (en) | Multifunctional bacterial biofilm generation device | |
| CN201512534U (en) | Blood culture vessel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |