CN103333790A - Novel aseptic plate medium and preparation method thereof - Google Patents
Novel aseptic plate medium and preparation method thereof Download PDFInfo
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- CN103333790A CN103333790A CN2013102819056A CN201310281905A CN103333790A CN 103333790 A CN103333790 A CN 103333790A CN 2013102819056 A CN2013102819056 A CN 2013102819056A CN 201310281905 A CN201310281905 A CN 201310281905A CN 103333790 A CN103333790 A CN 103333790A
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Abstract
The invention provides a novel aseptic plate medium and a preparation method thereof. The preparation method comprises following steps: step A, a piece of adhesive sticker is pasted on the lower surface of a plastic upper cover; step B, producing a lower part of the novel culture plate by using an injection molding machine; step C, filling a groove of the lower part with prepared liquid agar culture-medium, and cooling and solidifying the liquid agar culture-medium when the liquid agar culture-medium is spread at the bottom of the groove in a production line; step D, when the agar culture-medium is solidified, pressing an upper cover plastic film with the piece of adhesive sticker onto the low part of the novel culture plate in the production line, wherein the piece of adhesive sticker is ensured to be connected rightly to a right part of an extending part of the lower part of the novel culture plate and the culture plate is ensured to be sealed; and step E, piling the sealed aseptic plate medium up according packing specification, packing and encasing, and then sterilizing with gamma ray. The novel aseptic plate medium is specially designed, so that the upper cover does not need to be placed on the plate artificially, exposure time of the aseptic plate medium is short, and continuous large-scaled production can be realized. A limit of the number of each batch of prefabricated aseptic plate mediums is broke, so that production cost of the prefabricated aseptic plate medium can be reduced greatly.
Description
Technical field
The present invention relates to a kind of novel asepsis plate substratum and preparation method thereof.
Background technology
Aseptic plate substratum uses very general in pharmaceutical manufacturing, scientific research as a kind of traditional microorganism culturing, detection reagent, for example gnotobasis monitoring, microorganism culturing are identified etc.
The aseptic plate substratum of traditional self-control is generally used through the 90mm diameter culture dish of sterilization and pour 50-60 ℃ of liquid nutrient agar under gnotobasis, treats to seal after it cools off and makes.Shortcoming is total man worker's self-control, length consuming time, batch is little, stability is not high, storage time short.
The present general same use method for preparing of the prefabricated aseptic plate substratum of commercialization, make with three-layer plastic bag sealing back irradiation sterilization then, though comparing the self-control substratum increases in every respect, but still need manually add a cover or add a cover with machine, production lot is low, it is higher manually to reach hardware cost, and product structure itself exists not enough.
At present, no matter the testing laboratory in R﹠D institution, school, hospital, pharmaceutical manufacturing enterprise still is all kinds of production plants, the consumption of the aseptic plate substratum that Micro biological Tests uses is very huge, according to statistics, a production cycle or workshop regular environmental monitoring in every month of general bio-pharmaceuticals enterprise once just needs about 100 plate substratum.Generally there are two kinds of sources in the laboratory with substratum.
First kind of source be, user's oneself preparation substratum under aseptic condition, but this method not only operation is loaded down with trivial details, quantity is few, also very consuming time, be not enough to cope with contingencies, need fast, and to have bigger people be the microbiological contamination risk.
Second kind of source is to buy aseptic plate substratum outside, this method is time saving and energy saving, but the validity period of the aseptic plate substratum of market generally has only 3 months at present, and most of brands do not have independent sealed packing, the user can not correspond to actual needs in case Kaifeng just must use up the substratum of a packing unit at short notice.The product structure aspect adopts the cover cap embedding to be placed on the conventional combination mode on chassis mostly, brings certain risk for the gnotobasis in the aseptic plate substratum.On industrial scale, production efficiency is limited by the structure of product, and according to investigations, the producer that produces aseptic plate substratum on the market greatly is subject to below 5000 in batches, can not deal with the heavy demand of emergency event.
Summary of the invention
The objective of the invention is, invent a kind of sterile plate substratum structure, design form a complete production network equipment and technology, the prefabricated aseptic plate substratum of mass preparation.
A kind of novel asepsis plate substratum is characterized in that, comprising: loam cake, the following ware that is complementary with loam cake, following ware comprise groove and the epitaxial part that is connected with groove, and the upper cover part that contacts with epitaxial part scribbles non-setting adhesive.
Preferably, described groove is the cylinder shape groove of diameter 90mm, and thickness is 0.6mm.
Preferably, the ware of novel asepsis plate substratum adopts plastic material.
Preferably, described epitaxial part is a plane square, and length and width is 110mm, thickness 0.6mm, and it is circular-arc that four angles are cut to, and the edge at described four angles is mellow and full shape.
Preferably, described sunk part height is 15mm.
The present invention also provides a kind of method for preparing novel plate substratum, comprises following step:
Steps A: the lower surface that non-setting adhesive is printed on upper plastic cover;
Step B: make ware under the novel culture dish by injection moulding machine;
Step C: the liquid nutrient agar for preparing is poured in the described ware groove down, treat that at train line it is tiled in bottom portion of groove and cooled and solidified;
Step D: after nutrient agar solidifies, under the loam cake plastic film that will be printed on non-setting adhesive on the train line is impressed into, on the ware, guarantee the correct position that non-setting adhesive is connected with following ware epitaxial part, guarantee the sealing of culture dish;
Step e: the aseptic plate substratum of good seal is folded by packing specifications, and the packing vanning is sterilized by gamma ray.
Preferably, the wide of the roll of plastic of described steps A is 110mm, and thick is 0.2mm.
Preferably, among the described step D, the temperature of described liquid nutrient agar is 50～60 ℃ of insulations, packs in the described groove with the volume of 15～20ml/ ware.
Preferably, in the described step e, packing specifications is 10 ware/bags.
The present invention adopts above technical scheme, and beneficial effect is.
1. sterile plate substratum is simple for structure, and convenient the use realized that simultaneously extensive mechanize produces continuously.
2. each aseptic plate substratum is independent sealed, is convenient to adjust flexibly usage quantity.
3. the unique appearance structure design is convenient to user's mark and is taken.
At the above, the applicant makes following novelty by research and development and improves.
1, independent sealed.Adopt the chassis in the mode of can cooling back independent envelope film, both guaranteed the independent sterility of each plate substratum, lowered the risk of microbiological contamination in culturing process again greatly, use flexibly.
2, produce in enormous quantities.The process free of mechanize envelope film traditional staff method of adding a cover, realized production in enormous quantities greatly reducing production cost when having reduced the microbiological contamination risk in the production process, batch turnout reaches 50,000/batch more than.
3, easy to use.The epitaxial part of the lower cover of aseptic plate substratum is square, and four angles are circular-arc, the mellow and full hand that do not cut of edge polishing, and four jiaos of extensions have been avoided the interference of record vestige to experimental result as experimental record.
4, time of guaranteeing the quality is long.Substratum is installed in the lower cover by the machine branch through joining the filter back, through mechanize envelope film, packing after the subcooling, the unified irradiation sterilization that carries out then.This product preservation period under 2 ℃～25 ℃ conditions reaches 1 year, still keeps sterility and to the promotes growth of bacterium colony in preservation period.
5, suitability is wide.This production technique can be applied on more than the ten kind of solid medium, as nutrient agar, TSA substratum, Sabouraud's dextrose agar substratum, MH nutrient agar etc., application facet has contained the long inspection project of multiple Experiment on Microbiology such as escherichia coli, Pseudomonas aeruginosa, streptococcus aureus, subtilis, aspergillus niger, streptococcus albus, yeast bacterial classification.
With respect to prior art, because the design of novel asepsis plate substratum need not manually to add a cover, reduce substratum exposure duration among the present invention, can realize continuous large-scale production.Break through the restriction in batches of every batch of prefabricated aseptic plate substratum, can greatly reduce the production cost of prefabricated aseptic plate substratum.
Description of drawings
Fig. 1 is ware vertical view under the novel asepsis plate substratum of the present invention.
Fig. 2 is ware side-view under the novel asepsis plate substratum of the present invention.
Fig. 3 is novel asepsis plate substratum loam cake side upward view of the present invention.
Fig. 4 is that novel asepsis plate substratum of the present invention is added a cover side view cutaway drawing.
Embodiment
Below in conjunction with accompanying drawing, more excellent embodiment of the present invention is described in further detail:
Face is the technological process of production.
1, culture dish is plastics, and thickness is 0.6mm.
2, sunk part 20 is the cylinder shape groove of diameter 90mm.
3, epitaxial part 10 is a plane square, and length and width is 110mm, thickness 0.6mm, and it is circular-arc that four angles are cut to, the mellow and full hand that do not cut of edge polishing.
4, epitaxial part 10 is double-sided adhesive position 11, double-sided adhesive is printed on loam cake 30 lower surfaces, can be positioned this position after following ware and loam cake 30 sealings, and the adhesive power of non-setting adhesive and following ware is greater than the adhesive power of non-setting adhesive and loam cake 30, the non-setting adhesive of uncapping again will adhere to down the ware extension, separate with loam cake 30; Internal diameter is 96mm, and external diameter is 110mm, thickness 0.1mm.
Embodiment 2, as shown in Figure 2:
Sunk part 20 highly is 15mm, and epitaxial part 10 thickness are 0.6mm.
Embodiment 3, as shown in Figure 3 and Figure 4:
Culture dish loam cake 30 adopts plane squares, and length and width is 110mm, thickness 0.2mm, and it is circular-arc that four angles are cut to, the mellow and full hand that do not cut of edge polishing.
Double-sided adhesive is printed on loam cake 30 lower surfaces, and the adhesive power of non-setting adhesive and following ware is greater than the adhesive power of non-setting adhesive in loam cake 30, and the non-setting adhesive of uncapping again will adhere to down the ware extension, separate with loam cake 30; Internal diameter is 96mm, and external diameter is 110mm, and thickness is 0.1mm.
Embodiment 4:
As shown in Figure 4, the thickness of culture dish loam cake 30 is 0.2mm; The thickness of double-sided adhesive is 0.1mm; Epitaxial part 10 thickness are 0.6mm; The height of sunk part 20 is that 15mm, thickness are 0.6mm.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention does, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (9)
1. a novel asepsis plate substratum is characterized in that, comprising: loam cake, the following ware that is complementary with loam cake, and described ware down comprises groove and the epitaxial part that is connected with described groove, described epitaxial part links to each other with described loam cake by non-setting adhesive.
2. novel asepsis plate substratum as claimed in claim 1 is characterized in that, described groove is the cylinder shape groove of diameter 90mm, and thickness is 0.6mm.
3. novel asepsis plate substratum as claimed in claim 1 is characterized in that, described ware down adopts plastic material.
4. novel asepsis plate substratum as claimed in claim 1 is characterized in that described epitaxial part is plane square, and length and width is 110mm, thickness 0.6mm, and it is circular-arc that four angles are cut to, and the edge at described four angles is mellow and full shape.
5. novel asepsis plate substratum as claimed in claim 1 is characterized in that, the height of described sunk part is 15mm.
6. a method for preparing novel asepsis plate substratum as claimed in claim 1 is characterized in that, comprises following step:
Steps A: the lower surface that non-setting adhesive is printed on upper plastic cover;
Step B: make ware under the novel culture dish by injection moulding machine;
Step C: the liquid nutrient agar for preparing is poured in the described ware groove down, treat that at train line it is tiled in bottom portion of groove and cooled and solidified;
Step D: after nutrient agar solidifies, under the loam cake plastic film that will be printed on non-setting adhesive on the train line is impressed into, on the ware, guarantee the correct position that non-setting adhesive is connected with following ware epitaxial part, guarantee the sealing of culture dish;
Step e: the aseptic plate substratum of good seal is folded by packing specifications, and the packing vanning is sterilized by gamma ray.
7. method as claimed in claim 6 is characterized in that, the wide of the roll of plastic of described steps A is 110mm, and thick is 0.2mm.
8. method as claimed in claim 6 is characterized in that, among the described step D, described liquid asepsis nutrient agar is with 50～60 ℃ of insulations, packs in the described groove with the volume of 15～20ml/ ware.
9. method as claimed in claim 6 is characterized in that, in the described step e, adopting packing specifications is 10 ware/bags.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310281905.6A CN103333790B (en) | 2013-07-05 | 2013-07-05 | A kind of Novel aseptic plate medium and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310281905.6A CN103333790B (en) | 2013-07-05 | 2013-07-05 | A kind of Novel aseptic plate medium and preparation method thereof |
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| CN103333790A true CN103333790A (en) | 2013-10-02 |
| CN103333790B CN103333790B (en) | 2016-01-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937663A (en) * | 2014-05-08 | 2014-07-23 | 杭州北望生物技术有限公司 | Sterile culture medium plate for environment monitoring of antibiotic production workshop and preparation method of sterile culture medium plate |
| CN110607243A (en) * | 2019-10-22 | 2019-12-24 | 中秀科技股份有限公司 | Culture medium for separating fungi and yeast-like fungi and preparation method thereof |
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| CN202766533U (en) * | 2012-09-11 | 2013-03-06 | 青岛德瑞骏发生物科技有限公司 | Culture vessel |
| CN102978103A (en) * | 2012-03-06 | 2013-03-20 | 牛刚 | Manufacturing apparatus and manufacturing method for small sterile prefabricated culture dish |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US4280002A (en) * | 1979-12-03 | 1981-07-21 | Minnesota Mining And Manufacturing Company | Microbiological growth container |
| DE3218532A1 (en) * | 1982-05-17 | 1983-11-17 | C.A. Greiner und Söhne GmbH & Co KG, 7440 Nürtingen | Dish containing a nutrient medium for the cultivation of bacteria and small fungi |
| CN2031795U (en) * | 1988-04-26 | 1989-02-01 | 天津市第二医学院 | Thin-type plastic culture utensil |
| CN1083106A (en) * | 1992-08-25 | 1994-03-02 | 李建秋 | Disposable culture dish for anaerobe |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937663A (en) * | 2014-05-08 | 2014-07-23 | 杭州北望生物技术有限公司 | Sterile culture medium plate for environment monitoring of antibiotic production workshop and preparation method of sterile culture medium plate |
| CN110607243A (en) * | 2019-10-22 | 2019-12-24 | 中秀科技股份有限公司 | Culture medium for separating fungi and yeast-like fungi and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN103333790B (en) | 2016-01-20 |
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