CN103329942B - Compound disinfectant, and preparation method and applications thereof - Google Patents
Compound disinfectant, and preparation method and applications thereof Download PDFInfo
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- CN103329942B CN103329942B CN201310285600.2A CN201310285600A CN103329942B CN 103329942 B CN103329942 B CN 103329942B CN 201310285600 A CN201310285600 A CN 201310285600A CN 103329942 B CN103329942 B CN 103329942B
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- povidone iodine
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Abstract
The invention discloses applications of povidone-iodine and tea tree oil in preparation of an external combined antibacterial agent. The combined antibacterial agent comprises, by weight, 0.000125 to 12 portions of povidone-iodine and 0.015625 to 2 portions of tea tree oil. The invention also provides a compound disinfectant containing povidone-iodine and tea tree oil, and a preparation method thereof. The combination of povidone-iodine and tea tree oil possesses synergistic interaction, and is capable of increasing the antimicrobial activities of both povidone-iodine and tea tree oil. In addition, tea tree oil is gentle and fragrant, so that olfactory stimulation caused by povidone-iodine is reduced.
Description
Technical field
The present invention relates to a kind of compound disinfectant and its production and use.
Background technology
Povidone iodine (PVP-I) is a kind of precursor medicinal preparation, the amorphous compound of the one formed is combined with iodine by polyvinylpyrrolidone, containing available iodine 9% ~ 12%, for a kind of external sterilizing preservative of new type water-solubility iodine, compared with the tincture of iodine (iodine solution), there is stable in properties, good water solubility, nonirritant and volatility, that effect relaxes be lasting, the feature such as use safety, the easy wash-out of chromatogram, be a kind of disinfectant of efficient, wide spectrum, low toxicity.Its Antibacterial Mechanism is surface-active due to PVP-I and emulsification, on the one hand PVP-I penetrability is strengthened, emulsification makes cell wall damage on the other hand, and bacterial membrane permeability barrier destroys, PVP-I enters in cell in a large number, and born of the same parents' content spills and causes microbial death.The result of multinomial experimental study shows: PVP-I can kill the various pathogens such as common gram positive coccus, enteric bacilli, gram negative bacilli, gemma, virus, conveyor screw, anaerobic bacteria, vibrios, and seldom occurring that drug resistance is reacted, killing bacteria effect is better.But PVP-I is identical with other chemosterilant universal laws, within the specific limits, increase and extended durations of action with available iodine content, its bactericidal action strengthens.But after the available iodine of iodine-containing disinfectant reaches finite concentration, its bactericidal effect strengthens limited, even if particularly Iodophor class disinfectant concentration improves again, the ability of its kill bacteria gemma is not also obviously strengthened.
Along with developing rapidly of scale intensive culture industry, the generation also more sophisticated of Animal diseases.Utilize disinfectant to carry out disinfection to kill pathogene to livestock and poultry and surrounding environment thereof, cut off the routes of infection, become a kind of important measures of prevention and corntrol livestock and poultry disease transmission.But due to the continuous variation of infectious disease pathogens and the continuous appearance of drug resistance, the Disinfection Effect of many disinfecting drugs is also deteriorated gradually.And the excitant of most chemosterilant can to animal take sense of smell stress, thus cause that animal is uneasy, restless, unsmooth breath even affects and searches for food.Therefore critical effect is played in the scientific development of disinfectant and the purification work of reasonable utilization to plant.In order to play the effect of existing disinfecting drug better, exploitation compound disinfectant not only can utilize synergy between different disinfectant to reduce the generation of cause of disease drug resistance, and can extend the market potential of existing disinfectant further by covering the peculiar smell of disinfectant.
Yet there are no the relevant report by povidone iodine and natural antibacterial Drug combination.
Summary of the invention
The object of the present invention is to provide compound disinfectant containing povidone iodine and preparation method thereof.Another object of the present invention is to the drug combination purposes that povidone iodine and tea oil are provided.
The invention provides povidone iodine and the purposes of tea oil in the antibacterial external combination medicine of preparation; The weight proportion of described combination medicine is as follows: 1.25 × 10
-4~ 12 parts, tea oil 0.015625 ~ 2 part.
Further, the weight proportion of described combination medicine is as follows: povidone iodine 1.25 × 10
-4~ 40 × 10
-4part, tea oil 0.015625 ~ 2 part; Or povidone iodine 8 ~ 12 parts, tea oil 0.08 ~ 0.12 part.
Further, the weight proportion of described combination medicine is as follows: povidone iodine 10 parts, tea oil 0.1 part.
Wherein, described medicine is the medicine of Chinese People's Anti-Japanese Military and Political College enterobacteria or staphylococcus aureus.Present invention also offers a kind of compound disinfectant, it is prepared from by the bulk drug of following percentage by weight:
Povidone iodine 1.25 × 10
-4% ~ 12%, tea oil 0.015625% ~ 2%.
Further, it is prepared from by the bulk drug of following percentage by weight:
Povidone iodine 1.25 × 10
-4% ~ 40 × 10
-4%, tea oil 0.015625% ~ 2%; Or povidone iodine 8 ~ 12%, tea oil 0.08 ~ 0.12%.
Further, it is prepared from by the bulk drug of following percentage by weight: povidone iodine 10%, tea oil 0.1%.
Further, it is prepared from by following percentage by weight supplementary material:
Bulk drug: povidone iodine 8 ~ 12%, tea oil 0.08 ~ 0.12%;
Auxiliary material: Potassiumiodate 0.2 ~ 0.4%, solubilizer 1 ~ 3%, surplus are water.
Further, it is prepared from by following percentage by weight supplementary material:
Bulk drug: povidone iodine 10%, tea oil 0.1%;
Auxiliary material: Potassiumiodate 0.3%, solubilizer 2%, surplus are water.
Preferably, described solubilizer is selected from one or more the combination in Tween 80, polysorbate85, polysorbate60, polyoxyethylene 40 fatty acid ester, lauryl sodium sulfate, PLURONICS F87.
Present invention also offers the preparation method of above-mentioned compound disinfectant, it comprises following operating procedure:
(1) supplementary material is taken by weight percentage:
(2) tea oil is mixed with solubilizer, add in appropriate water and prepare solution A; Povidone iodine is dissolved in suitable quantity of water, adds Potassiumiodate and mix, preparation solution B; Solution A, B are mixed, constant volume.
The present invention, by after povidone iodine and tea oil conbined usage, enhances both antibacterial activities, has played synergistic function; The simultaneously warm fragrance of tea oil, can alleviate the sense of smell that povidone iodine brings in use stress.
Embodiment
The present invention's tea oil used meets tea oil international standard ISO4730-1996.
The preparation of embodiment 1 compound disinfectant of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, Tween 80, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 10%, tea oil 0.1%, Tween 80 2%, Potassiumiodate 0.3%, and surplus is water.
The preparation method of povidone iodine and tea oil compound disinfectant: mixed with Tween 80 by tea oil, adds in appropriate water, forms settled solution.Povidone iodine is dissolved in suitable quantity of water, adds Potassiumiodate and mix.Then tea oil solution and Betagen Solution are mixed, constant volume.
Wherein, Tween 80 is as the solubilizer of tea oil, and in making it soluble in water, thus realize the Homogeneous phase mixing with Betagen Solution, solubilizer is not limited only to Tween 80, as long as can play good solubilising; Potassiumiodate adds the iodate ion concentration in solution, makes iodine and water react the reaction generating iodate ion and not easily carries out, thus add the stability of povidone iodine.
The preparation of embodiment 2 compound disinfectant of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, Tween 80, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 8%, tea oil 0.12%, Tween 80 3%, Potassiumiodate 0.4%, and surplus is water.Preparation method is with reference to embodiment 1.
The preparation of embodiment 3 compound disinfectant of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, Tween 80, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 12%, tea oil 0.08%, Tween 80 1%, Potassiumiodate 0.2%, and surplus is water.Preparation method is with reference to embodiment 1.
The preparation of embodiment 4 compound disinfectant of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, lauryl sodium sulfate, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 12%, tea oil 0.08%, lauryl sodium sulfate 1%, Potassiumiodate 0.2%, and surplus is water.Preparation method is with reference to embodiment 1.
The preparation of embodiment 5 compound disinfectant of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, PLURONICS F87, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 12%, tea oil 0.08%, PLURONICS F87 1%, Potassiumiodate 0.2%, and surplus is water.Preparation method is with reference to embodiment 1.
Beneficial effect of the present invention is illustrated below by way of test example.
Test example 1 povidone iodine and tea oil are to the Combination susceptibility testing of fraction of pathogens bacterium
In order to investigate povidone iodine and whether tea oil has synergetic antibacterial effect to pathogenic microorganism, the Combination susceptibility testing of povidone iodine and tea oil that utilized chessboard method to carry out.
2.2.1 pathogen
Escherichia coli reference culture; Staphylococcus aureus reference culture
2.2.2 medium
Ordinary broth liquid nutrient medium (Hangzhou microorganism reagent Co., Ltd); Agar medium (Hangzhou microorganism reagent Co., Ltd).
2.2.3 doubling dilution measures tea oil and the povidone iodine MIC value to staphylococcus aureus and Escherichia coli reference culture
The bacterium liquid of preparation is carried out count plate, and is diluted to containing bacterium (5 × 10 with 0.03mol/LPBS
5~ 5 × 10
6) the test organisms liquid of cfu/ml.96 orifice plates are soaked 2 hours in strong acid, and taking-up is rinsed well, dries, uses ultraviolet irradiation before use 2 hours.Each Kong Jun adds 100 μ L ordinary broths, and povidone iodine and tea oil respectively test 4 rows.Povidone iodine distilled water is mixed with the solution of debita spissitudo, be mixed with the aqueous solution after tea oil Tween 80 solubilising simultaneously, respectively get Betagen Solution and tea oil solution 100 μ L and add the first corresponding hole respectively, draw 100 μ L after mixing and add second hole, same method is diluted to the 11 hole successively, discards 100 μ L.12 hole does not add medicine as positive control.Then each Kong Jun adds the staphylococcus aureus bacterium liquid of 100 μ L, 96 orifice plates is placed in 37 DEG C of insulating boxs and cultivates observed result after 18h.With the minimum drug concentration in the micropore of energy bacteria growing inhibiting for minimal inhibitory concentration (MIC), average.Be measured in the same method povidone iodine and tea oil to colibacillary minimal inhibitory concentration (MIC).It is 10mg/L that result records povidone iodine to the minimum inhibitory concentration of staphylococcus aureus, is 5mg/L to colibacillary minimum inhibitory concentration.Tea oil is 7.5mg/ml to the minimum inhibitory concentration of staphylococcus aureus, is 5mg/ml to colibacillary minimum inhibitory concentration.
2.2.4 the chessboard method associating drug sensitive experiment of tea oil and povidone iodine
For the Combination susceptibility testing of Escherichia coli to tea oil and povidone iodine, be 80mg/ml(16MIC by the concentration dilution of tea oil in cillin bottle
tea oil), 40mg/ml, 20mg/ml, 10mg/ml, 5mg/ml, 2.5mg/ml, 1.25mg/ml, 0.625mg/ml, be 160 μ g/ml(32MIC by the concentration dilution of povidone iodine
pVP-I), 50 μ L ordinary broths are respectively added in (A-H) × (1-8) hole of 96 orifice plates, then arrange at A the povidone iodine that each hole adds the 160 μ g/ml of 50 μ L, doubling dilution is diluted to H row successively, discard 50 μ L, then add the tea oil solution of 8mg/ml, 4mg/ml, 2mg/ml, 1mg/ml, 0.5mg/ml, 0.25mg/ml, 0.125mg/ml, 0.0625mg/ml of 50 μ L in the 1 to the 8 each hole of row respectively; The last 50 μ L that respectively add in (A-H) × (1-8) hole of 96 orifice plates are containing bacterial context soup.Take out after 96 orifice plates being placed in 37 DEG C of insulating boxs cultivation 18h and read result.
The same to the Combination susceptibility testing method of staphylococcus aureus.Every bacterial strain repeats chessboard and tests three times.
The basis for estimation of experimental result using part Mlc (FIC) index as associating antibacterial tests.FIC index calculation method is as follows: FIC index=MIC first medicine coupling/MIC first prescription+MIC second medicine coupling/MIC second prescription is used.FIC≤0.5 is synergy, and 0.5 < FIC≤1 is summation action, and 1 < FIC≤2 are irrelevant effect, and FIC > 2 is antagonism.Identical test in triplicate, results averaged.
Tea oil and povidone iodine coupling to the suppression situation of Escherichia coli and staphylococcus aureus in table 1 and table 2.
Table 1 tea oil and povidone iodine are to colibacillary associating drug sensitivity tests
Note: "-" represents do not have bacterial growth; "+" indicates bacterial growth.
As can be seen from Table 1, after tea oil and povidone iodine conbined usage, its MIC all there occurs change.Wherein the MIC of povidone iodine is reduced to 1.25 μ g/ml, and the MIC of tea oil is reduced to 1.25mg/ml.Tea oil can be calculated and povidone iodine coupling to colibacillary FIC index is: FIC index=1.25/5+1.25/5=0.5 according to the result of table 1, illustrate that tea oil and povidone iodine coupling have synergy to colibacillary suppression, the two coupling can eliminate the impact of Escherichia coli on breeding environment better as disinfectant.
Table 2 tea oil and povidone iodine are to the associating drug sensitivity tests of staphylococcus aureus
Note: "-" represents do not have bacterial growth; "+" indicates bacterial growth.
Tea oil can be calculated and the FIC index of povidone iodine coupling to staphylococcus aureus is: FIC index=5/10+1.88/7.5=0.75 according to the result of table 2, illustrate that tea oil and the suppression of povidone iodine coupling to staphylococcus aureus have summation action, the two coupling can eliminate the impact of staphylococcus aureus on breeding environment as disinfectant.
The data that table 1 and table 2 provide finally just calculate its FIC index to ask, thus both judging, whether conbined usage has synergy, and in table, MIC data are not as unique foundation of the two conbined usage ratio.As result confirms there is synergy, as long as the concentration of the two is higher than the MIC value of its conbined usage, the result of Synergistic all can be reached.The proportioning of every "-" in table, can bacteria growing inhibiting both all illustrating, therefore the proportioning of every "-" all can be used as the ratio reference of Clinical practice.Consider the concrete condition of Clinical practice and cost, so determine the ratio of aforementioned combinations use.According to the measurement result of MIC after above-mentioned conbined usage, the concentration of suggestion tea oil is 0.15625 ~ 20mg/ml or more (if consider cost factor, tea oil concentration can be slightly low), and povidone iodine is at 1.25 ~ 40 μ g/ml or more.
In sum, the present invention, by after povidone iodine and tea oil conbined usage, has played synergistic function, enhances both antibacterial activities, effectively can reduce the working concentration of povidone iodine, reduces chemical drug cost; The simultaneously warm fragrance of tea oil, can alleviate the sense of smell that povidone iodine brings in use stress.
Claims (2)
1. povidone iodine and the purposes of tea oil in the anticolibacillary medicine of the external associating of preparation; The concentration of described povidone iodine is 1.25-40 μ g/ml, and the concentration of tea oil is 1.25-20mg/ml.
2. povidone iodine and the tea oil purposes in the medicine of the external associating anti-Staphylococcus aureus of preparation; The concentration of described povidone iodine is 5-80 μ g/ml, and the concentration of tea oil is 1.88-30mg/ml.
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| CN103329942B true CN103329942B (en) | 2015-07-15 |
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| CN109169722A (en) * | 2018-07-11 | 2019-01-11 | 李玉茹 | A kind of animal husbandry thimerosal and preparation method thereof |
| CN109362797A (en) * | 2018-12-04 | 2019-02-22 | 怀化学院 | Iodic disinfecting liquid and preparation method thereof |
| CN112314640A (en) * | 2020-08-03 | 2021-02-05 | 吴丹 | Compound disinfectant with high adsorption performance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257716A (en) * | 1998-12-18 | 2000-06-28 | 赵成 | Pudendum-cleaning hygienic cream |
| CN1478810A (en) * | 2002-08-28 | 2004-03-03 | 周维文 | Perfume antibacteria degradation polyolefin composition and its preparation method and use |
| CN102846725A (en) * | 2012-03-21 | 2013-01-02 | 华南农业大学 | Compound povidone iodine suppository and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257716A (en) * | 1998-12-18 | 2000-06-28 | 赵成 | Pudendum-cleaning hygienic cream |
| CN1478810A (en) * | 2002-08-28 | 2004-03-03 | 周维文 | Perfume antibacteria degradation polyolefin composition and its preparation method and use |
| CN102846725A (en) * | 2012-03-21 | 2013-01-02 | 华南农业大学 | Compound povidone iodine suppository and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 山茶油在治疗和预防新生儿红臀中的应用与观察;兰美倩等;《中国民族民间医药》;20101231;第19卷(第17期);第38页 * |
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