CN103329942A - Compound disinfectant, and preparation method and applications thereof - Google Patents
Compound disinfectant, and preparation method and applications thereof Download PDFInfo
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- CN103329942A CN103329942A CN2013102856002A CN201310285600A CN103329942A CN 103329942 A CN103329942 A CN 103329942A CN 2013102856002 A CN2013102856002 A CN 2013102856002A CN 201310285600 A CN201310285600 A CN 201310285600A CN 103329942 A CN103329942 A CN 103329942A
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- tea oil
- povidone iodine
- iodine
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- povidone
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Abstract
The invention discloses applications of povidone-iodine and tea tree oil in preparation of an external combined antibacterial agent. The combined antibacterial agent comprises, by weight, 0.000125 to 12 portions of povidone-iodine and 0.015625 to 2 portions of tea tree oil. The invention also provides a compound disinfectant containing povidone-iodine and tea tree oil, and a preparation method thereof. The combination of povidone-iodine and tea tree oil possesses synergistic interaction, and is capable of increasing the antimicrobial activities of both povidone-iodine and tea tree oil. In addition, tea tree oil is gentle and fragrant, so that olfactory stimulation caused by povidone-iodine is reduced.
Description
Technical field
The present invention relates to a kind of compound disinfectant and its production and use.
Background technology
Povidone iodine (PVP-I) is a kind of pro-drug preparation, be combined a kind of amorphous compound that forms with iodine by polyvinylpyrrolidone, contain available iodine 9%～12%, external sterilizing preservative for a kind of new type water-solubility iodine, compare with the tincture of iodine (iodine solution), have stable in properties, good water solubility, nonirritant and volatility, effect relax lasting, use characteristics such as safety, the easy wash-out of chromatogram, be the disinfectant of a kind of efficient, wide spectrum, low toxicity.Its antibiotic mechanism is because surface-active and the emulsification of PVP-I, the PVP-I penetrability is strengthened, and emulsification is destroyed cell wall on the other hand, and the bacterial membrane permeability barrier destroys, PVP-I enters in the cell in a large number, and born of the same parents' content spills and causes microbial death.The result of multinomial experimental study shows: PVP-I can kill various pathogens such as common gram positive coccus, enteric bacilli, gram negative bacilli, gemma, virus, conveyor screw, anaerobic bacteria, vibrios, and the drug resistance reaction seldom occurs, the killing bacteria effect is better.But PVP-I is identical with other chemosterilant universal laws, within the specific limits, increases and prolongation action time with available iodine content, and its bactericidal action strengthens.But the available iodine that contains iodine disinfectant reaches after the finite concentration, and its bactericidal effect strengthens limited, even particularly Iodophor class disinfectant concentration improves again, the ability of its kill bacteria gemma is not obviously strengthened yet.
Along with developing rapidly of scale intensive culture industry, the generation of Animal diseases is more sophisticated also.Utilize disinfectant that livestock and poultry and surrounding environment thereof are carried out disinfection to kill pathogene, cut off the routes of infection, become a kind of important measures of prevention and control livestock and poultry disease transmission.But because continuous variation and the chemical sproof continuous appearance of infectious disease cause of disease, the Disinfection Effect of many disinfecting drugs is variation gradually also.And the excitant of most chemosterilants can to animal take sense of smell stress, thereby cause that animal uneasiness, restless, unsmooth breath even influence search for food.Therefore the scientific development of disinfectant and utilization are rationally played critical effect to the purification work of plant.For the effect of the existing disinfecting drug of performance better, the exploitation compound disinfectant not only can utilize the synergy between the different disinfectants to reduce the chemical sproof generation of cause of disease, and can further prolong the market potential of existing disinfectant by the peculiar smell of covering disinfectant.
Yet there are no the relevant report with povidone iodine and the medication combined use of natural antibacterial.
Summary of the invention
The object of the present invention is to provide compound disinfectant that contains povidone iodine and preparation method thereof.Another object of the present invention is to provide the drug combination purposes of povidone iodine and tea oil.
The invention provides povidone iodine and the tea oil purposes in the antibacterial external combination medicine of preparation; The weight proportion of described combination medicine is as follows: 1.25 * 10
-40.015625～2 part of～12 part, tea oil.
Further, the weight proportion of described combination medicine is as follows: povidone iodine 1.25 * 10
-4～40 * 10
-40.015625～2 part of part, tea oil; Or 8～12 parts of povidone iodine, 0.08～0.12 part of tea oil.
Further, the weight proportion of described combination medicine is as follows: 10 parts of povidone iodine, 0.1 part of tea oil.
Wherein, described medicine is the medicine of Chinese People's Anti-Japanese Military and Political College enterobacteria or staphylococcus aureus.The present invention also provides a kind of compound disinfectant, and it is to be prepared from by following raw materials by weight percent medicine:
Povidone iodine 1.25 * 10
-4%～12%, tea oil 0.015625%～2%.
Further, it is to be prepared from by following raw materials by weight percent medicine:
Povidone iodine 1.25 * 10
-4%～40 * 10
-4%, tea oil 0.015625%～2%; Or povidone iodine 8～12%, tea oil 0.08～0.12%.
Further, it is to be prepared from by following raw materials by weight percent medicine: povidone iodine 10%, tea oil 0.1%.
Further, it is to be prepared from by following percentage by weight supplementary material:
Bulk drug: povidone iodine 8～12%, tea oil 0.08～0.12%;
Auxiliary material: Potassiumiodate 0.2～0.4%, solubilizer 1～3%, surplus are water.
Further, it is to be prepared from by following percentage by weight supplementary material:
Bulk drug: povidone iodine 10%, tea oil 0.1%;
Auxiliary material: Potassiumiodate 0.3%, solubilizer 2%, surplus are water.
Preferably, described solubilizer is selected from one or more the combination in Tween 80, polysorbate85, polysorbate60, polyoxyethylene 40 fatty acid esters, lauryl sodium sulfate, the poloxamer 188.
The present invention also provides the preparation method of above-mentioned compound disinfectant, and it comprises following operating procedure:
(1) take by weighing supplementary material by weight percentage:
(2) tea oil and solubilizer are mixed, add in an amount of water and prepare solution A; Povidone iodine is dissolved in the suitable quantity of water, adds Potassiumiodate and mix, the preparation solution B; Solution A, B are mixed, and constant volume gets final product.
The present invention has strengthened both antibacterial activities after povidone iodine and tea oil are united use, has brought into play synergistic function; The warm fragrance of tea oil simultaneously, can alleviate the sense of smell that povidone iodine brings in use stress.
Embodiment
The used tea oil of the present invention meets tea oil international standard ISO4730-1996.
The preparation of embodiment 1 compound disinfectant of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, Tween 80, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 10%, and tea oil 0.1%, Tween 80 2%, Potassiumiodate 0.3%, surplus is water.
The preparation method of povidone iodine and tea oil compound disinfectant: tea oil and Tween 80 are mixed, add in an amount of water, form settled solution.Povidone iodine is dissolved in the suitable quantity of water, adds Potassiumiodate and mix.Then tea oil solution and Betagen Solution are mixed, constant volume gets final product.
Wherein, Tween 80 is as the solubilizer of tea oil, make its soluble in water in, thereby realize mixing with the even of Betagen Solution, solubilizer is not limited only to Tween 80, as long as can bring into play good solubilising; Potassiumiodate has increased the iodate ion concentration in the solution, and the reaction that makes the reaction of iodine and water generate iodate ion is difficult for carrying out, thereby has increased the stability of povidone iodine.
The preparation of embodiment 2 compound disinfectants of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, Tween 80, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 8%, and tea oil 0.12%, Tween 80 3%, Potassiumiodate 0.4%, surplus is water.The preparation method is with reference to embodiment 1.
The preparation of embodiment 3 compound disinfectants of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, Tween 80, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 12%, and tea oil 0.08%, Tween 80 1%, Potassiumiodate 0.2%, surplus is water.The preparation method is with reference to embodiment 1.
The preparation of embodiment 4 compound disinfectants of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, lauryl sodium sulfate, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 12%, and tea oil 0.08%, lauryl sodium sulfate 1%, Potassiumiodate 0.2%, surplus is water.The preparation method is with reference to embodiment 1.
The preparation of embodiment 5 compound disinfectants of the present invention
Compound disinfectant of the present invention is made up of povidone iodine, tea oil, poloxamer 188, Potassiumiodate, and its percentage by weight is as follows: povidone iodine 12%, and tea oil 0.08%, poloxamer 1881%, Potassiumiodate 0.2%, surplus is water.The preparation method is with reference to embodiment 1.
Below specify beneficial effect of the present invention by testing example.
Test example 1 povidone iodine and tea oil are to the associating drug sensitive test of part pathogen
Whether pathogenic microorganism there is synergetic antibacterial effect in order to investigate povidone iodine and tea oil, utilizes chessboard method to carry out the associating drug sensitive test of povidone iodine and tea oil.
2.2.1 pathogen
The Escherichia coli reference culture; The staphylococcus aureus reference culture
2.2.2 medium
Ordinary broth liquid nutrient medium (Hangzhou microorganism reagent Co., Ltd); Agar medium (Hangzhou microorganism reagent Co., Ltd).
2.2.3 doubling dilution mensuration tea oil and povidone iodine are to the MIC value of staphylococcus aureus and Escherichia coli reference culture
The bacterium liquid of preparation is carried out count plate, and be diluted to 0.03mol/LPBS and contain bacterium (5 * 10
5～5 * 10
6) the test organisms liquid of cfu/ml.96 orifice plates were soaked in strong acid 2 hours, and taking-up is rinsed well, and oven dry is faced with preceding ultraviolet irradiation 2 hours of using.Each hole all adds 100 μ L ordinary broths, and povidone iodine and tea oil are respectively tested 4 rows.Povidone iodine is mixed with the solution of debita spissitudo with distilled water, simultaneously tea oil is mixed with the aqueous solution after with the Tween 80 solubilising, respectively get Betagen Solution and tea oil solution 100 μ L and add the first corresponding hole respectively, draw 100 μ L behind the mixing and add second hole, same method is diluted to the 11 hole successively, discards 100 μ L.The 12 hole does not add medicine as positive control.Each hole all adds the staphylococcus aureus bacterium liquid of 100 μ L then, places 37 ℃ of insulating boxs to cultivate observed result behind the 18h 96 orifice plates.Be minimal inhibitory concentration (MIC) with the minimum drug concentration in the micropore that can bacteria growing inhibiting, average.Measure povidone iodine and tea oil to colibacillary minimal inhibitory concentration (MIC) with method.It is 10mg/L to the minimum inhibitory concentration of staphylococcus aureus that the result records povidone iodine, is 5mg/L to colibacillary minimum inhibitory concentration.Tea oil is 7.5mg/ml to the minimum inhibitory concentration of staphylococcus aureus, is 5mg/ml to colibacillary minimum inhibitory concentration.
2.2.4 the chessboard method of tea oil and povidone iodine associating drug sensitive experiment
Be example with Escherichia coli to the associating drug sensitive test of tea oil and povidone iodine, the concentration dilution with tea oil in cillin bottle is 80mg/ml(16MIC
Tea oil), 40mg/ml, 20mg/ml, 10mg/ml, 5mg/ml, 2.5mg/ml, 1.25mg/ml, 0.625mg/ml, be 160 μ g/ml(32MIC with the concentration dilution of povidone iodine
PVP-I), in (A-H) * (1-8) hole of 96 orifice plates, respectively add 50 μ L ordinary broths, arrange the povidone iodine that each hole adds the 160 μ g/ml of 50 μ L at A then, doubling dilution is diluted to H row successively, discard 50 μ L, add 8mg/ml, 4mg/ml, 2mg/ml, 1mg/ml, 0.5mg/ml, the 0.25mg/ml of 50 μ L, the tea oil solution of 0.125mg/ml, 0.0625mg/ml then in the 1st to 8 each hole of row respectively; At last in (A-H) * (1-8) hole of 96 orifice plates, respectively add 50 μ L and contain bacterial context soup.96 orifice plates are placed 37 ℃ of insulating boxs to cultivate to take out behind the 18h read the result.
Associating drug sensitive test method to staphylococcus aureus is the same.Every bacterial strain repeats chessboard test three times.
Experimental result is with the basis for estimation of part Mlc (FIC) index as the associating antibacterial tests.The FIC index calculation method is as follows: FIC index=MIC first medicine coupling/MIC first prescription usefulness+MIC second medicine coupling/MIC second prescription is used.FIC≤0.5 is synergy, and 0.5＜FIC≤1 is summation action, and 1＜FIC≤2 are irrelevant effect, and FIC＞2 are antagonism.The identical test triplicate, results averaged.
Tea oil and povidone iodine coupling see Table 1 and table 2. to the inhibition situation of Escherichia coli and staphylococcus aureus
Table 1 tea oil and povidone iodine are to colibacillary associating drug sensitivity tests
Annotate: "-" expression does not have bacterial growth; "+" expression has bacterial growth.
As can be seen from Table 1, after tea oil and povidone iodine were united use, variation had all taken place in its MIC.Wherein the MIC of povidone iodine is reduced to 1.25 μ g/ml, and the MIC of tea oil is reduced to 1.25mg/ml.Can calculate tea oil and the povidone iodine coupling to colibacillary FIC index is: FIC index=1.25/5+1.25/5=0.5 according to the result of table 1, illustrate that tea oil and povidone iodine coupling have synergy to colibacillary inhibition, the two coupling can be eliminated Escherichia coli to the influence of breeding environment better as disinfectant.
Table 2 tea oil and povidone iodine are to the associating drug sensitivity tests of staphylococcus aureus
Annotate: "-" expression does not have bacterial growth; "+" expression has bacterial growth.
Can calculate tea oil and povidone iodine coupling according to the result of table 2 to the FIC index of staphylococcus aureus is: FIC index=5/10+1.88/7.5=0.75, illustrate that tea oil and povidone iodine coupling have summation action to the inhibition of staphylococcus aureus, the two coupling can be eliminated staphylococcus aureus to the influence of breeding environment as disinfectant.
The data that table 1 and table 2 provide are finally just calculated its FIC index in order to ask, thereby judge that the two unites use and whether have synergy, and the MIC data are as unique foundation of the two associating usage ratio in the table.Confirm that as the result synergy is arranged, as long as the concentration of the two is higher than the MIC value that it unites use, all can reach the result of Synergistic.The proportioning of every "-" in the table illustrates that all the two can bacteria growing inhibiting, and therefore the proportioning of every "-" all can be used as the ratio reference of clinical use.Consider the concrete condition of clinical use and cost, so determined aforementioned ratio of uniting use.According to the above-mentioned measurement result of using back MIC of uniting, the concentration of suggestion tea oil is 0.15625～20mg/ml or above (if consider cost factor, tea oil concentration can be low slightly), povidone iodine 1.25～40 μ g/ml or more than.
In sum, the present invention has brought into play synergistic function after povidone iodine and tea oil are united use, has strengthened both antibacterial activities, can effectively reduce the working concentration of povidone iodine, reduces the chemical drug cost; The warm fragrance of tea oil simultaneously, can alleviate the sense of smell that povidone iodine brings in use stress.
Claims (10)
1. povidone iodine and tea oil are in the purposes of preparation in the external associating antibacterials; The weight proportion of described combination medicine is as follows: povidone iodine 1.25 * 10
-40.015625～2 part of～12 part, tea oil.
2. purposes according to claim 1, it is characterized in that: the weight proportion of described combination medicine is as follows: povidone iodine 1.25 * 10
-4～40 * 10
-40.015625～2 part of part, tea oil; Or 8～12 parts of povidone iodine, 0.08～0.12 part of tea oil.
3. purposes according to claim 1 and 2, it is characterized in that: the weight proportion of described combination medicine is as follows: 10 parts of povidone iodine, 0.1 part of tea oil.
4. according to any described purposes of claim 1～3, it is characterized in that: described medicine is the medicine of Chinese People's Anti-Japanese Military and Political College enterobacteria or staphylococcus aureus.
5. compound disinfectant, it is characterized in that: it is to be prepared from by following raw materials by weight percent medicine:
Povidone iodine 1.25 * 10
-4%～12%, tea oil 0.015625%～2%.
6. compound disinfectant according to claim 5, it is characterized in that: it is to be prepared from by following raw materials by weight percent medicine:
Povidone iodine 1.25 * 10
-4%～40 * 10
-4%, tea oil 0.015625%～2%; Or povidone iodine 8～12%, tea oil 0.08～0.12%.
7. according to claim 5 or 6 described compound disinfectants, it is characterized in that: it is to be prepared from by following percentage by weight supplementary material:
Bulk drug: povidone iodine 8～12%, tea oil 0.08～0.12%;
Auxiliary material: Potassiumiodate 0.2～0.4%, solubilizer 1～3%, surplus are water.
8. compound disinfectant according to claim 7, it is characterized in that: it is to be prepared from by following percentage by weight supplementary material:
Bulk drug: povidone iodine 10%, tea oil 0.1%;
Auxiliary material: Potassiumiodate 0.3%, solubilizer 2%, surplus are water.
9. according to claim 6 or 7 described compound disinfectants, it is characterized in that: described solubilizer is selected from one or more the combination in Tween 80, polysorbate85, polysorbate60, polyoxyethylene 40 fatty acid esters, lauryl sodium sulfate, the poloxamer 188.
10. the preparation method of any described compound disinfectant of claim 6～9, it is characterized in that: it comprises following operating procedure:
(1) take by weighing supplementary material by weight percentage:
(2) tea oil and solubilizer are mixed, add in an amount of water and prepare solution A; Povidone iodine is dissolved in the suitable quantity of water, adds Potassiumiodate and mix, the preparation solution B; Solution A, B are mixed, and constant volume gets final product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169722A (en) * | 2018-07-11 | 2019-01-11 | 李玉茹 | A kind of animal husbandry thimerosal and preparation method thereof |
| CN109362797A (en) * | 2018-12-04 | 2019-02-22 | 怀化学院 | Iodic disinfecting liquid and preparation method thereof |
| CN112314640A (en) * | 2020-08-03 | 2021-02-05 | 吴丹 | Compound disinfectant with high adsorption performance |
Citations (3)
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| CN1257716A (en) * | 1998-12-18 | 2000-06-28 | 赵成 | Pudendum-cleaning hygienic cream |
| CN1478810A (en) * | 2002-08-28 | 2004-03-03 | 周维文 | Perfume antibacteria degradation polyolefin composition and its preparation method and use |
| CN102846725A (en) * | 2012-03-21 | 2013-01-02 | 华南农业大学 | Compound povidone iodine suppository and preparation method and application thereof |
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2013
- 2013-07-09 CN CN201310285600.2A patent/CN103329942B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1257716A (en) * | 1998-12-18 | 2000-06-28 | 赵成 | Pudendum-cleaning hygienic cream |
| CN1478810A (en) * | 2002-08-28 | 2004-03-03 | 周维文 | Perfume antibacteria degradation polyolefin composition and its preparation method and use |
| CN102846725A (en) * | 2012-03-21 | 2013-01-02 | 华南农业大学 | Compound povidone iodine suppository and preparation method and application thereof |
Non-Patent Citations (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169722A (en) * | 2018-07-11 | 2019-01-11 | 李玉茹 | A kind of animal husbandry thimerosal and preparation method thereof |
| CN109362797A (en) * | 2018-12-04 | 2019-02-22 | 怀化学院 | Iodic disinfecting liquid and preparation method thereof |
| CN112314640A (en) * | 2020-08-03 | 2021-02-05 | 吴丹 | Compound disinfectant with high adsorption performance |
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|---|---|
| CN103329942B (en) | 2015-07-15 |
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