CN103329735A - Method for manufacturing edible fungi test tube stock by utilizing rice as culture medium - Google Patents
Method for manufacturing edible fungi test tube stock by utilizing rice as culture medium Download PDFInfo
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- CN103329735A CN103329735A CN2013103064640A CN201310306464A CN103329735A CN 103329735 A CN103329735 A CN 103329735A CN 2013103064640 A CN2013103064640 A CN 2013103064640A CN 201310306464 A CN201310306464 A CN 201310306464A CN 103329735 A CN103329735 A CN 103329735A
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Abstract
The invention belongs to the field of biotechnology, and particularly relates to a method for manufacturing edible fungi test tube stock by utilizing rice as a culture medium. The method comprises the following steps: selecting particulate rice free of mildew and worm damage as raw material; preparing nutrient solution consisting of white sugar, lime, monopotassium phosphate and magnesium sulfate; soaking the rice in the nutrient solution until rice grains absorb enough water; then, after steaming the rice, stirring a proper amount of corn flour and subpackaging in a test tube; and finally performing sterilization, inoculation and cultivation according to conventional methods so as to obtain the test tube stock with high quality. Through the implementation of the method, the used raw material are wide in source, rich in nutrient and low in cost; and moreover, the equipment is simple, the manufacturing process is simple and convenient, easy to grasp, labor-saving and time-saving. The prepared test tube stock is suitable for the seed production of various mushroom types, and the hypha after strain transfer is quick for growth, neat and robust. Meanwhile, the amount of prepared rice microbial strains is greater than that of slant microbial strain pre-culture spawn bottles (bags).
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of rice that utilizes and make the female method of planting of edible mushroom test tube as medium.
Background technology
In in the parent edible fungus kind production process, normally adopt potato, glucose, agar to prepare medium (being PDA).The advantage of this method is nutritious, is fit to multiple eating bacterium bacterial classification, and is convenient, fast during inoculation, suitable a large amount of production.But this method material therefor cost is higher, and the preparation process program is many, complex production process, wastes time and energy.In addition, during reality was produced, a lot of Edible Fungi fields (factory) on a small scale and family workshop be in order to reduce production costs, and generally is to introduce a 1--2 from some for kind of unit to prop up mother and plant voluntarily tube and enlarge female quantity of planting and satisfy the production needs.But small scale generally is used for the limited amount of subculture tube because these are individual, so, make female kind of test tube with the training of PDA inclined-plane and be not suitable for very much.
Summary of the invention
Purpose of the present invention is exactly to overcome the deficiencies in the prior art and provide brand-new a kind of rice that utilizes to make the female method of planting of edible mushroom test tube as medium.Specifically comprise: select particle not have to go mouldy, the rice of free from insect pests is as raw material, the nutrient solution that preparation is made up of white sugar, lime, potassium dihydrogen phosphate, magnesium sulfate, suction moisture with being soaked in the nutrient solution after the rice until the grain of rice, admix an amount of corn flour after then rice being cooked and be sub-packed in the test tube, sterilize according to a conventional method at last, inoculation, cultivation and female kind of test tube of high-quality.By implementing the present invention, raw materials used source is wide, and is nutritious, and cost is low, and related equipment is simple, and manufacturing process is easy, easily grasps, not only saving of labor but also save time.The test tube for preparing is female plants the production of hybrid seeds that is applicable to various mushroom classes, and bacterial classification switching back mycelial growth is rapid and neat healthy and strong.Simultaneously, it is many that the rice bacterial classification for preparing connects original seed bottle (bag) quantity than slant strains; A slant strains can expand female 60-80 of kind props up, and expands original seed 3-5 bottle (bag); And the rice kind can expand 80-100 and props up female the kind, expands original seed 5-7 bottle (bag).Because the inclined-plane kind is only with the long mycelia part in top layer, and medium stays, the rice kind can all be used.
The present invention is achieved through the following technical solutions:
A kind of rice that utilizes is made the female method of planting of edible mushroom test tube as medium, comprises the steps: in the technical scheme
1, feedstock capture: select not have go mouldy, the rice of free from insect pests is as raw material for standby.
2, preparation nutrient solution: add 10 gram white sugar, 10 gram lime, 5 gram potassium dihydrogen phosphates, 2 gram magnesium sulfate in 1 kg of water, above-mentioned each component is dissolved in the water fully and to get nutrient solution standby.
3, raw material is handled: earlier rice is soaked the end and also continue to soak 8～10 hours in nutrient solution, pick up then and clean with clear water immediately, then rice is cooked, mix an amount of corn flour at last and thus cooked rice grains is loosed off standby.
4, raw material tubulature: loose cooked rice grains branch is filled to half of test tube, then cotton is soaked in the nutrient solution, pick up again and remove redundant moisture, keep the water content of cotton 55～60%, then cotton is compressed rice in the pipe, thus rice is fixed in test tube, cleans outer wall in vitro simultaneously, and toward test tube mouth tampon beyond the Great Wall.
5, test tube sterilization: test tube is positioned in the disinfecting container, and it is standby to carry out sterilization according to conventional method then.
6, inoculation cultivation: the test tube through sterilization is moved to inoculating hood, under gnotobasis, insert edible fungus species, also be cultured to female kind of test tube that mycelia is covered with test tube and get high-quality then according to a conventional method.
Compared with prior art, the existing following advantage of the present invention:
1, edible mushroom test tube mother proposed by the invention plants preparation method's measure uniqueness, and technology is simple, easily implements.
2, by implementing the present invention, replacing expensive agar to make the edible mushroom mother culture media with rice, is a kind of not only economy but also practical method.
3, by implementing the present invention, raw material sources are wide, and are nutritious, and cost is low, and related equipment is simple, and manufacturing process is easy, easily grasp, not only saving of labor but also save time.
4, by implementing the present invention, the female kind of the test tube for preparing is applicable to the production of hybrid seeds of various mushroom classes, and bacterial classification is transferred the back mycelial growth rapidly and neat stalwartness.
5, by implementing the present invention, it is many that the rice bacterial classification for preparing connects original seed bottle (bag) quantity than slant strains; A slant strains can expand female 60-80 of kind props up, and expands original seed 3-5 bottle (bag); And the rice kind can expand 80-100 and props up female the kind, expands original seed 5-7 bottle (bag).Because the inclined-plane kind is only with the long mycelia part in top layer, and medium stays, the rice kind can all be used.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
A kind of rice that utilizes is made the female method of planting of edible mushroom test tube as medium, and embodiment is as follows:
1, feedstock capture: select not have go mouldy, the rice of free from insect pests is as raw material for standby.
2, preparation nutrient solution: add 10 gram white sugar, 10 gram lime, 5 gram potassium dihydrogen phosphates, 2 gram magnesium sulfate in 1 kg of water, above-mentioned each component is dissolved in the water fully and to get nutrient solution standby.
3, raw material is handled: earlier rice is soaked the end and also continue to soak 8～10 hours in nutrient solution, pick up then and clean with clear water immediately, then rice is cooked, mix an amount of corn flour at last and thus cooked rice grains is loosed off standby.
4, raw material tubulature: loose cooked rice grains branch is filled to half of test tube, then cotton is soaked in the nutrient solution, pick up again and remove redundant moisture, keep the water content of cotton 55～60%, then cotton is compressed rice in the pipe, thus rice is fixed in test tube, cleans outer wall in vitro simultaneously, and toward test tube mouth tampon beyond the Great Wall.
5, test tube sterilization: test tube is positioned in the disinfecting container, and it is standby to carry out sterilization according to conventional method then.
6, inoculation cultivation: the test tube through sterilization is moved to inoculating hood, under gnotobasis, insert edible fungus species, also be cultured to female kind of test tube that mycelia is covered with test tube and get high-quality then according to a conventional method.
Claims (1)
1. one kind is utilized rice to make the female method of planting of edible mushroom test tube as medium, and its feature comprises the steps:
(1) feedstock capture: select not have go mouldy, the rice of free from insect pests is as raw material for standby;
(2) preparation nutrient solution: add 10 gram white sugar, 10 gram lime, 5 gram potassium dihydrogen phosphates, 2 gram magnesium sulfate in 1 kg of water, above-mentioned each component is dissolved in the water fully and to get nutrient solution standby;
(3) raw material is handled: earlier rice is soaked the end and also continue to soak 8～10 hours in nutrient solution, pick up then and clean with clear water immediately, then rice is cooked, mix an amount of corn flour at last and thus cooked rice grains is loosed off standby;
(4) raw material tubulature: loose cooked rice grains branch is filled to half of test tube, then cotton is soaked in the nutrient solution, pick up again and remove redundant moisture, keep the water content of cotton 55～60%, then cotton is compressed rice in the pipe, thus rice is fixed in test tube, cleans outer wall in vitro simultaneously, and toward test tube mouth tampon beyond the Great Wall;
(5) test tube sterilization: test tube is positioned in the disinfecting container, and it is standby to carry out sterilization according to conventional method then;
(6) inoculation cultivation: the test tube through sterilization is moved to inoculating hood, under gnotobasis, insert edible fungus species, also be cultured to female kind of test tube that mycelia is covered with test tube and get high-quality then according to a conventional method.
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| CN2013103064640A CN103329735A (en) | 2013-07-19 | 2013-07-19 | Method for manufacturing edible fungi test tube stock by utilizing rice as culture medium |
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| CN2013103064640A CN103329735A (en) | 2013-07-19 | 2013-07-19 | Method for manufacturing edible fungi test tube stock by utilizing rice as culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106957852A (en) * | 2017-03-29 | 2017-07-18 | 上海市农业科学院 | A kind of asparagus method for transformation using the grain of rice as culture matrix |
| CN108419611A (en) * | 2018-05-22 | 2018-08-21 | 福建农林大学 | A kind of culture medium of cracking rice cultivates method and the application of agrocybe |
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| CN102417376A (en) * | 2010-09-28 | 2012-04-18 | 姜广宇 | Method for preparing seed culture medium by utilizing corn and paddy particles |
| CN102090268A (en) * | 2010-12-18 | 2011-06-15 | 福建嘉田农业开发有限公司 | Edible fungus strain liquefying inoculation method utilizing grain culture medium as matrix |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106957852A (en) * | 2017-03-29 | 2017-07-18 | 上海市农业科学院 | A kind of asparagus method for transformation using the grain of rice as culture matrix |
| CN106957852B (en) * | 2017-03-29 | 2020-11-06 | 上海市农业科学院 | Flammulina velutipes transformation method using rice grains as culture medium |
| CN108419611A (en) * | 2018-05-22 | 2018-08-21 | 福建农林大学 | A kind of culture medium of cracking rice cultivates method and the application of agrocybe |
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