CN103323399B - A kind of micro-nano fiber biosensor - Google Patents
A kind of micro-nano fiber biosensor Download PDFInfo
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- CN103323399B CN103323399B CN201310213431.1A CN201310213431A CN103323399B CN 103323399 B CN103323399 B CN 103323399B CN 201310213431 A CN201310213431 A CN 201310213431A CN 103323399 B CN103323399 B CN 103323399B
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Abstract
A kind of micro-nano fiber biosensor, relates to optical field, is specifically related to a kind of biology sensor.It is complex structure in order to solve the existing real-time living body measurement apparatus to biosome Brillouin spectrum, poor stability, problem that accuracy is low.The laser that its laser instrument sends is incident to coupling mechanism, is divided into a light beam and No. two light beams through coupling mechanism, adopts single side-band modulator modulate a light beam and be incident to a light input end of circulator through micro-nano fiber; The intensity of electrooptic modulator to No. two light beams is modulated, and is incident to No. two light input ends of circulator after Erbium-Doped Fiber Amplifier (EDFA) amplifies; The light of the light output end outgoing of circulator is incident to the photosensitive end of photodetector; The electric signal of photodetector exports oscillograph to through lock-in amplifier.The present invention is applicable to the real-time somatometry to biosome Brillouin spectrum.
Description
Technical field
The present invention relates to optical field, be specifically related to a kind of biology sensor.
Background technology
Spontaneous brillouin scattering is a kind of inelastic scatter, and it originates from noncoherent density fluctuation or phonon in medium.Brillouin spectrum reflects the spectrum change in scattering process, and directly provides the phonon information relevant to medium bullet light characteristic.Directly can calculate the velocity of sound by Brillouin shift, can elastic constant be calculated by the velocity of sound, the anisotropy about the velocity of sound can be obtained by the change of the velocity of sound, the information of relaxation process and phase transformation.Phonon damping process can be studied by Brillouin linewidth (high-resolution device need be used), obtain the viscosity property of material.The coupling etc. of phonon and electronic state can be studied according to the measurement of intensity.At biomedical sector, the bullet light characteristic that Brillouin light spectroscopy has been successfully applied to vitro samples is measured, and this comprises collagenous fibres, bone, cornea and crystalline lens, and hemorheologic parameters etc.
The way of traditional measurement Brillouin spectrum directly measures spontaneous brillouin scattering, but the elastic scattering light that the difficult point of this technology is how to distinguish spontaneous brillouin scattering light and originates from Rayleigh Scattering and Mie Scattering.In most of biological substance and solution, the intensity of elastic light scattering can than the several magnitude of Brillouin scattering height, and this will certainly affect the measuring accuracy of spontaneous brillouin scattering spectrum.In addition, Brillouin shift very little (only having GHz magnitude), uses traditional spectrometer to differentiate.By contrast, stimulated Brillouin scattering light spectrum based on pumping-detection technology can significantly improve spectral resolution and signal to noise ratio (S/N ratio), and this technology is insensitive to background light radiation, namely the zero-frequency place of moving of measure spectrum does not have Rayleigh scattering signal, and the measuring error of the Brillouin shift can ignored because angular deviation causes and live width.If but adopted the Brillouin spectrum of pumping-detection commercial measurement biological substance liquid in free space, would then need pump light and detection light strictly to aim at, cause complex structure, poor stability; And the hot spot that the maintenance cannot growing distance is in free space less, because this reducing brillouin gain and signal to noise ratio (S/N ratio), this labyrinth also cannot realize the real-time somatometry to biosome in addition.
Micro-nano fiber is an important research field in nanophotonics, and it becomes microminiaturized, the integrated alternative elementary cell of one of following optical device with the performance of its brilliance.When light transmits in micro-nano fiber, part energy is distributed in outside micro-nano fiber, forms evanescent wave, utilizes evanescent wave to the sensitivity characteristic of surrounding medium refractive index, can realize polytype micro-nano fiber biosensor.
Summary of the invention
The present invention is complex structure in order to solve the existing real-time living body measurement apparatus to biosome Brillouin spectrum, poor stability, problem that accuracy is low, thus provides a kind of micro-nano fiber biosensor.
A kind of micro-nano fiber biosensor, it comprises laser instrument 1, coupling mechanism 2, microwave source 3, single side-band modulator 4, scrambler 5, liquid storage equipment 6, micro-nano fiber 7, circulator 8, Erbium-Doped Fiber Amplifier (EDFA) 9, electrooptic modulator 10, function generator 11, photodetector 12, lock-in amplifier 13 and oscillograph 14
Liquid storage equipment 6 inside is filled with solution to be measured; Micro-nano fiber 7 is arranged in the solution of liquid storage equipment 6;
The laser that laser instrument 1 sends is incident to coupling mechanism 2, is divided into a light beam and No. two light beams through coupling mechanism 2, and a described light beam is incident to single side-band modulator 4, and microwave source 3 drives single side-band modulator 4 to modulate a light beam, obtains detection light; Described detection light is incident to one end of micro-nano fiber 7; Described detection light penetrates at the other end of micro-nano fiber 7 and is incident to a light input end of circulator 8;
No. two light beams are incident to electrooptic modulator 10, function generator 11 drives the intensity of electrooptic modulator 10 to No. two light beams to modulate, obtain the pump light of copped wave, the pump light of described copped wave is incident to No. two light input ends of circulator 8 after Erbium-Doped Fiber Amplifier (EDFA) 9 amplifies;
The light of the light output end outgoing of circulator 8 is incident to the photosensitive end of photodetector 12; The electrical signal of described photodetector 12 is connected with the detectable signal input end of lock-in amplifier 13; The reference signal output terminal of the reference signal input end function generator 11 of described lock-in amplifier 13 connects; The signal output part of described lock-in amplifier 13 is connected with the signal input part of oscillograph 14.
Beneficial effect of the present invention:
1, the present invention utilizes micro-nano fiber to fetter and conducts pump light and detection light, make light field with the form vast scale of evanescent wave penetrate into around it liquid medium in there is stimulated Brillouin scattering and interact, and then being coupled in standard single-mode fiber, the structure of all-fiber makes that system architecture is simple, flexible operation;
2, micro-nano fiber size is less, can imbed biological substance inside easily and carry out real-time monitored in vivo;
3, the micro-nano fiber of quartz material has nontoxic, constitutionally stable feature, can not pollute biological substance and destroy.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is micro-nano fiber schematic cross-section in the solution; Wherein marking 15 is solution;
Fig. 3 is the structural representation of the micro-nano fiber in solution; Wherein marking A is adiabatic tapered zone; Mark B is even micro-nano fiber.
Embodiment
Embodiment one, composition graphs 1 to Fig. 3 illustrate this embodiment, a kind of micro-nano fiber biosensor, it comprises laser instrument 1, coupling mechanism 2, microwave source 3, single side-band modulator 4, scrambler 5, liquid storage equipment 6, micro-nano fiber 7, circulator 8, Erbium-Doped Fiber Amplifier (EDFA) 9, electrooptic modulator 10, function generator 11, photodetector 12, lock-in amplifier 13 and oscillograph 14
Liquid storage equipment 6 inside is filled with solution to be measured; Micro-nano fiber 7 is arranged in the solution of liquid storage equipment 6;
The laser that laser instrument 1 sends is incident to coupling mechanism 2, is divided into a light beam and No. two light beams through coupling mechanism 2, and a described light beam is incident to single side-band modulator 4, and microwave source 3 drives single side-band modulator 4 to modulate a light beam, obtains detection light; Described detection light is incident to one end of micro-nano fiber 7; Described detection light penetrates at the other end of micro-nano fiber 7 and is incident to a light input end of circulator 8;
No. two light beams are incident to electrooptic modulator 10, function generator 11 drives the intensity of electrooptic modulator 10 to No. two light beams to modulate, obtain the pump light of copped wave, the pump light of described copped wave is incident to No. two light input ends of circulator 8 after Erbium-Doped Fiber Amplifier (EDFA) 9 amplifies;
The light of the light output end outgoing of circulator 8 is incident to the photosensitive end of photodetector 12; The electrical signal of described photodetector 12 is connected with the detectable signal input end of lock-in amplifier 13; The reference signal output terminal of the reference signal input end function generator 11 of described lock-in amplifier 13 connects; The signal output part of described lock-in amplifier 13 is connected with the signal input part of oscillograph 14.
The difference of a kind of micro-nano fiber biosensor described in embodiment two, this embodiment and embodiment one is, laser instrument 1 is narrow-line width single frequency optical fiber laser.
The difference of a kind of micro-nano fiber biosensor described in embodiment three, this embodiment and embodiment one or two is, coupling mechanism 2 is 50: 50 coupling mechanisms.
The difference of a kind of micro-nano fiber biosensor described in embodiment four, this embodiment and embodiment three is, detects frequency frequency Brillouin shift lower than No. two light beams of light.
The difference of a kind of micro-nano fiber biosensor described in embodiment five, this embodiment and embodiment one, two or four is, the two ends of micro-nano fiber 7 are gradual pyramidal structure.
Principle of work: the present invention adopts narrow-line width single frequency optical fiber laser as light source, output wavelength is the continuous light of 1550nm.Pumping-detection commercial measurement Brillouin spectrum needs two difference on the frequency lockings and adjustable light source, the light exported for this laser instrument is divided into two-way, wherein a road is directly as pump light, another road is conduct detection light after the modulation of microwave-driven single side-band modulator, its frequency about Brillouin shift lower than pump light frequency, the microwave frequency exported by changing microwave source can realize the scanning detecting light frequency easily.The scheme utilizing microwave-driven single side-band modulator to obtain detection light and pump light is except having the advantage of tuning simple, compact conformation, because pump light and detection light are from same light source, therefore their frequency difference intrinsic is stablized, the not impact of Stimulated Light frequency drift, can solve frequency difference lockout issue therebetween well.Use function generator to drive electro-optic intensity modulator to carry out to pump light the pump light that intensity modulated obtains copped wave, function generator is also for lock-in amplifier provides reference signal simultaneously.The model of function generator is: Imtech AFG3252.Pump light after copped wave enters micro-nano fiber district by a circulator after being amplified by Erbium-Doped Fiber Amplifier (EDFA) again.Polarization correlated due to Brillouin amplification process, pump light or the change of detection polarisation of light state can change brillouin gain, use an optical fiber scrambler to change detection polarisation of light state randomly for this reason, changes the signal to noise ratio (S/N ratio) reduction caused in order to avoid polarization state.Detection light after amplification is through photoelectric detector and change, then after lock-in amplifier process, improves signal to noise ratio (S/N ratio), finally by oscillograph recording amplifying signal.
The present invention has following advantage:
1, utilize micro-nano fiber to fetter in the present invention and conduct pump light and detection light, make light field with the form vast scale of evanescent wave penetrate into around it liquid medium in there is stimulated Brillouin scattering and interact, and then being coupled in standard single-mode fiber, the structure of all-fiber makes that system architecture is simple, flexible operation;
2, micro-nano fiber size is less, can imbed biological substance inside easily and carry out real-time monitored in vivo;
3, the micro-nano fiber of quartz material has nontoxic, constitutionally stable feature, can not pollute biological substance and destroy.
Claims (5)
1. a micro-nano fiber biosensor, it is characterized in that: it comprises laser instrument (1), coupling mechanism (2), microwave source (3), single side-band modulator (4), scrambler (5), liquid storage equipment (6), micro-nano fiber (7), circulator (8), Erbium-Doped Fiber Amplifier (EDFA) (9), electrooptic modulator (10), function generator (11), photodetector (12), lock-in amplifier (13) and oscillograph (14)
Liquid storage equipment (6) inside is filled with solution to be measured; Micro-nano fiber (7) is arranged in the solution of liquid storage equipment (6);
The laser that laser instrument (1) sends is incident to coupling mechanism (2), a light beam and No. two light beams are divided into through coupling mechanism (2), a described light beam is incident to single side-band modulator (4), microwave source (3) drives single side-band modulator (4) to modulate a light beam, obtains detection light; Described detection light is incident to one end of micro-nano fiber (7); Described detection light penetrates at the other end of micro-nano fiber (7) and is incident to a light input end of circulator (8);
No. two light beams are incident to electrooptic modulator (10), function generator (11) drives electrooptic modulator (10) intensity to No. two light beams to modulate, obtain the pump light of copped wave, the pump light of described copped wave is incident to No. two light input ends of circulator (8) after Erbium-Doped Fiber Amplifier (EDFA) (9) amplifies;
The light of the light output end outgoing of circulator (8) is incident to the photosensitive end of photodetector (12); The electrical signal of described photodetector (12) is connected with the detectable signal input end of lock-in amplifier (13); The reference signal output terminal of reference signal input end function generator (11) of described lock-in amplifier (13) connects; The signal output part of described lock-in amplifier (13) is connected with the signal input part of oscillograph (14);
This micro-nano fiber biosensor utilizes micro-nano fiber to fetter and conduction pump light and detection light, makes light field penetrate in solution to be measured with the form vast scale of evanescent wave stimulated Brillouin scattering occurs interact, and then to be coupled in standard single-mode fiber.
2. a kind of micro-nano fiber biosensor according to claim 1, is characterized in that laser instrument (1) is narrow-line width single frequency optical fiber laser.
3. a kind of micro-nano fiber biosensor according to claim 1 and 2, is characterized in that coupling mechanism (2) is for 50:50 coupling mechanism.
4. a kind of micro-nano fiber biosensor according to claim 3, is characterized in that frequency frequency Brillouin shift lower than No. two light beams of detection light.
5. a kind of micro-nano fiber biosensor according to claim 1,2 or 4, is characterized in that the two ends of micro-nano fiber (7) are gradual pyramidal structure.
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| CN103645551B (en) * | 2013-12-18 | 2016-05-25 | 江苏大学 | A kind of micro-nano fiber assembly and manufacture method thereof |
| CN104568764A (en) * | 2015-01-28 | 2015-04-29 | 哈尔滨工业大学 | Optical fiber evanescent wave form quartz enhanced photoacoustic spectrum sensor and gas measurement method |
| CN104729958B (en) * | 2015-03-19 | 2017-09-19 | 杭州电子科技大学 | The optical fiber microsensor and detecting system of in-site detecting biofilm density distribution |
| CN105006739B (en) * | 2015-07-16 | 2018-02-09 | 盐城工学院 | A kind of Brillouin scattering dynamic raster generation device and method based on capillary |
| CN105136177B (en) * | 2015-08-27 | 2017-09-05 | 太原理工大学 | The distribution type optical fiber sensing equipment and method of a kind of submillimeter spatial resolution |
| CN109781154B (en) * | 2019-02-14 | 2021-06-22 | 哈尔滨工业大学 | Brillouin correlation domain analysis system with high spatial resolution based on intensity modulation |
| CN109856057B (en) * | 2019-02-27 | 2021-10-29 | 哈尔滨工业大学 | Liquid component detection device and method with tapered optical fiber as medium |
| CN114235016A (en) * | 2021-12-06 | 2022-03-25 | 哈尔滨工业大学 | Dynamic BOTDA sensing method and system based on injection locking high-order sideband output |
| CN115373079B (en) * | 2022-08-05 | 2024-07-16 | 香港理工大学深圳研究院 | All-optical photothermal polarization modulator and system based on micro-nano optical fiber asymmetric mode field |
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