CN103316051A - New functions and applications of trametes lactinea(Berk.)Pat. or extractives and trametenloic acid B - Google Patents
New functions and applications of trametes lactinea(Berk.)Pat. or extractives and trametenloic acid B Download PDFInfo
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- CN103316051A CN103316051A CN2013101762667A CN201310176266A CN103316051A CN 103316051 A CN103316051 A CN 103316051A CN 2013101762667 A CN2013101762667 A CN 2013101762667A CN 201310176266 A CN201310176266 A CN 201310176266A CN 103316051 A CN103316051 A CN 103316051A
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Abstract
The invention relates to new functions and applications of trametes lactinea(Berk.)Pat. or extractives and trametenloic acid B. The substance trametes lactinea(Berk.)Pat has the functions of digestive gastric ulcer resistance, and nonulcerative-indigestion resistance through increasing of gastric motility. The trametes lactinea(Berk.)Pat. or the extractives and the trametenloic acid B have certain inbibitional effects on cell proliferation of gastric cancer cells, esophagus cancer cells Eca-109 and colorectal cancer cells LOVO, and has a wide range of research value and mass production and edible value in dugs for digestive system and gastric cancer resisting and the like.
Description
Technical field
The application relates to the purposes of Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, relates in particular to its purposes in pharmacy and health food.
Background technology
Trametes laclinea Berk Trametes lactinea (Berk.) Pat. is a kind of macro fungi of Aphyllophorales Polyporaceae Trametes
[1], experiment with Trametes laclinea Berk in the soil common food Preserved-fish of Tujia Pepper from, be accredited as Trametes laclinea Berk through teacher Wang Shaobai.Few to the research of Trametes laclinea Berk both at home and abroad.In recent years, Trametes laclinea Berk be studies show that the Trametes laclinea Berk extract has good immunological enhancement and antitumor action.
Summary of the invention
Use EIMS instrument (VG Analytical70-250S, 70eV) that the extract of Trametes laclinea Berk is carried out molecular weight determination, the nuclear magnetic resonance data of the chemical compound that obtains extracting is
The nuclear magnetic resonance data of table 1 Trametes laclinea Berk extract 3-hydrogenation pine Siberian cocklebur acid B
From the EIMS collection of illustrative plates of chemical compound, can see molecular ion [M]
+454, determine that thus its molecular weight is 454, other fragment peak (439[M ± CH
3]
+(100), 421[M ± CH
3± H
2O]
+(33), 393 (43), 297 (48), 95 (38), 69 (43)), with Francesca Cateni[1] etc. the data of report coincide.
The mass spectrum and the nuclear magnetic resonance map data that obtain are made comparisons with data in literature, identify main compound, 3-hydrogenation pine Siberian cocklebur acid B (3 beta-hydroxy Pilus Caprae seu Ovis steroids-8,24-diene-21-acid), the structural formula of the sour B of 3-hydrogenation pine Siberian cocklebur is as shown in Figure 1.
This chemical compound is extracted by Trametes laclinea Berk and makes, known act on diabetes active component.
The object of the invention is to prepare Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, and be used for the application of the medicine of prevention and treatment peptic ulcer.
Another object of the present invention is to prepare Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, and be applied to prevent and treat application in the medicine of non-ucler dyspepsia.
Another object of the present invention is to prepare Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, and be applied to prevent and treat application in the medicine of gastric cancer.
A further object of the present invention is to prepare Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, and is applied to prevent and treats application in the medicine of the esophageal carcinoma.
A further object of the present invention is to prepare Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, and is applied to prevent and treats application in the medicine of colorectal cancer.
A further object of the present invention is to prepare Trametes laclinea Berk silk or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B, and is applied to the application in health food, the medicine.
Trametes laclinea Berk silk of the present invention or Trametes laclinea Berk extract, 3-hydrogenation pine Siberian cocklebur acid B have inhibition H
+, K
+-ATPase activity and anti-ulcer effect.
Description of drawings
Fig. 1 is the structural formula of 3-hydrogenation pine Siberian cocklebur acid B.
Fig. 2 is 3-hydrogenation pine Siberian cocklebur acid B and H
+, K
+-ATPase molecular docking result.
Specific embodiment
The preparation method of Trametes laclinea Berk mycelia, DABAI bolt extract 3-hydrogenation pine Siberian cocklebur acid B, DABAI bolt water extract, 95% ethanol extraction is seen artificial culture method and the purposes of patent application Trametes laclinea Berk, and publication number is: 200810046969.7.
1. anti-peptic ulcer test
(1) suppresses H
+, K
+-ATPase active function: get normal kunming mice, body weight 20-25g, non-fasting, the cervical vertebra dislocation is put to death, and the 5min that sterilizes in 75% ethanol dissects under the aseptic condition, and the stomach bag is taken out, and places cold PBS buffer salt solution.Coating protein matter on the adventitia and fat etc. is wiped off when getting stomach as far as possible.With shears the stomach bag is prolonged and large curvedly to cut off part from lower ending opening, again with tweezers with the upset of stomach bag, clamp and do not cut off end, food debris is washed to the greatest extent temporarily preservation in cold PBS buffer salt solution with the PBS buffer salt solution.With 5% the pancreatin solution for preparing, the consumption according to each stomach bag 4-5ml digests.The gastric parietal cell is scraped, be distributed in the cold pancreatin of taking-up.The cold pancreatin that is dispersed with the theca interna cell is put into 37 ℃ of water-baths digest 10min.In the digestion process every 2-3min gently jolting once prevent the cell accumulation that digests.After digestion is finished, under aseptic condition, blow and beat gently with liquid-transfering gun, make cell coming off as much as possible.With 200 purpose screen clothes cell and tissue are separated.Cell sap is changed in the 10ml centrifuge tube, and centrifugal 8min discards solution under 1200rpm, adds rapidly the DMEM culture medium.
Above-mentioned cell is made single cell suspension, adjust cell concentration to 1 * 10 with the DMEM culture medium
5About/ml, be inoculated in 96 orifice plates, every hole 100 μ l.Add respectively omeprazole (10ug/ml), 95% ethanol extraction (80,40 and 20ug/ml), DABAI bolt water extract (80,40 and 20ug/ml) 3-hydrogenation pine Siberian cocklebur acid B(40,20 and 10ug/ml), (add medicine and all add the culture medium dilution with concentration after less than 0.1% DMSO dissolving, through 0.22 μ m membrane filtration), 37 ℃ hatch 4h after, every kind of drug level amounts to three parallel assay samples, uses H
+, K
+-ATPase determination of activity kit measurement enzymatic activity is calculated suppression ratio.
Table 2 Trametes laclinea Berk extract is to H
+, K
+The impact of-ATPase activity
With matched group than * p ﹤ 0.05, * * p ﹤ 0.01
(2) anti-ulcer effect: get Kunming mouse, random minute in groups, every group of 10 male and female half and half.Test group gavage every day gives relative medicine, totally five days, water is can't help in fasting after the administration in the 4th day, after the last administration after 1 hour, gavage aspirin-alcoholic solution 0.2ml(with aspirin 200mg/kg with 40% dissolve with ethanol), put to death mice behind the 4h, mice is cut off along the greater gastric curvature place, with clear water flush away content, under anatomic microscope, observe the gastric ulcer point after the upset, score according to lesion degree.Contrafluxion is rubescent to be 1 minute, and petechial hemorrhage or erosion respectively are 1 minute, and rotten to the corn 1 of wire is 3 minutes, amounts to rear as ulcer index.Get simultaneously gastric tissue homogenate, measure enzyme and live.
Table 3 Trametes laclinea Berk extract is on the impact of mouse gastric ulcer
With model group than * p ﹤ 0.05, * * p ﹤ 0.01
Experiment in vivo and vitro result demonstration, Trametes laclinea Berk has the anti-peptic ulcer effect, and DABAI bolt extract, 3-hydrogenation pine Siberian cocklebur acid B are main effective ingredient.
Molecular docking is calculated: in the Auto Dock4.0 software, make up respectively H
+, K
+-ATPase(receptor) and the three dimensional structure of 3-hydrogenation pine Siberian cocklebur acid B (part), with part and receptor docking, operating software is checked the docking result.The docking result can represent by △ G with combination with the docking score value, by 3-hydrogenation pine Siberian cocklebur acid B and the H of Fig. 2
+, K
+-ATPase molecular docking result is H as can be known
+, K
+The combination of-ATPase and 3-hydrogenation pine Siberian cocklebur acid B can △ G=-4.75.
2. improve the test of gastric motility
The low rat model of gastric motility is made:
After the SD rat is bought, adaptability is raised 3d, random packet, blank group and model group give respectively normal saline and the glycyrrhiza uralensis (10g/kg) of 2ml, the next day half amount take food, continue 10 days, make the low rat model of gastric motility, after the modeling success, model group continues to give Radix Glycyrrhizae solution, Normal group to giving the equivalent normal saline, the medicine group gives relative medicine, and continues gavage Radix Glycyrrhizae solution every day (10g/kg).Add phenol red solution 0.8ml during the 7th day gavage, after gavage finished 20min, rat was put to death in the cervical vertebra dislocation, cuts abdominal part open, and ligation cardia and pylorus are won stomach and small intestinal.
Stomach residue rate and intestinal propulsion rate are measured:
Stomach residue rate: get 0.002g/ml phenol red solution 1ml and be added in the NaOH solution of 10ml0.1mol/L, the concussion mixing.Get 0.2ml and be dissolved in the 1ml pure water, be titer.Ultraviolet-uisible spectrophotometer is titer OD value in measuring its absorbance under the wavelength 560nm.Full stomach is dipped in the NaOH solution of 0.1mol/L and shreds, fully washing shakes up, the centrifugal 10min of 4000rpm under the room temperature collects supernatant 0.2ml, adds pure water 1ml, the same ultraviolet-uisible spectrophotometer that uses is measured its again absorbance under the 560nm, is and measures liquid OD value.Calculate as follows Stomach residue rate:
Stomach residue rate (%)=mensuration liquid OD value/titer OD value * 100%.
The intestinal propulsion rate: the intercepting pylorus is tiled in and dissects on the plate to far-end ileocecus part, measures total length.Observe the phenol red forward position to the distance of pylorus and measure its length.If can not know the demonstration phenol red, then cut off intestinal tube from far-end, syringe drips 0.1mol/L NaOH, if the shown in red phenol red of then pointing out of color is advanced into herein.The percent that accounts for the small intestinal total length with phenol red propelling length at small intestinal calculates the intestinal propulsion rate.
Intestinal propulsion rate (%)=phenol red advance distance/small intestinal total length (cm) * 100%.
Table 4 Trametes laclinea Berk extract is on the impact of Stomach residue rate and intestinal propulsion rate
With model group than * p ﹤ 0.05, * * p ﹤ 0.01
The experimental result demonstration, Trametes laclinea Berk can increase gastric motility, and DABAI bolt extract, 3-hydrogenation pine Siberian cocklebur acid B are main effective ingredient.
Gastric motility refers to the contraction peristaltic forces of stomach muscle, comprises strength and frequency that stomach muscle is shunk.Gastric motility disorder is the main cause that causes non-ucler dyspepsia.When obstacle appears in gastric motility, dyspepsia can occur, abdominal distention, belch, inappetence, acid regurgitation occur, feel sick, the indigestion symptom such as vomiting.Trametes laclinea Berk can increase gastric motility, can be used for treating the dyspepsia that gastric dynamic dysfunction causes.
3. anti-Effect on Gastric Cancer test
The In vitro culture of cell strain: people's gastric epithelial cell GES-1 and gastric carcinoma cells HGC-27 DMEM culture medium culturing, it is 10% that cell culture fluid all contains the hyclone volume fraction, contains each 100U/ml of penicillin and streptomycin.All cells is cultivated in 37 ℃, 5%CO2 incubator, and the trypsin digestion and cell with 0.25% goes down to posterity, and the trophophase cell of taking the logarithm is used for experiment.
3-hydrogenation pine Siberian cocklebur acid B under different pH value on the impact of cell survival rate:
Single cell suspension is made in the trophophase cell routine of taking the logarithm digestion, adjusts cell concentration to 5 * 10 with culture fluid
4-1 * 10
5About ml, be inoculated in 96 orifice plates, every hole 100 μ l.Add 3-hydrogenation pine Siberian cocklebur acid B(1 μ g/ml, 5 μ g/ml and 10 μ g/ml after cultivating 24h), Trametes laclinea Berk ethanol extract (20 μ g/ml and 40 μ g/ml) and Trametes laclinea Berk water extract (20 μ g/ml and 40 μ g/ml), other establishes blank group and Normal group, every group arranges different pH value (pH7.5 simultaneously, pH6.5 and pH5.5), establish 6 multiple holes for every group.37 ℃ are continued to cultivate, and cultivate 24h, after, every hole adds 5mg/ml MTT20 μ l, hatch 4h for 37 ℃, discard culture fluid, every hole adds DMSO150 μ l, slightly shake, crystal is fully dissolved, detect the trap (OD value) in every hole with enzyme-linked immunosorbent assay instrument at wavelength 492nm place.
Calculate inhibitory rate of cell growth by following formula: the average OD value of the average OD value/Normal group of inhibitory rate of cell growth (%)=1-(dosing group) * 100%.
Adopt SPSS13.0 software to carry out statistical analysis, experimental data with
The expression, with P＜0.05 for having significant difference.
Table 5 medicine under different PH on the impact of HGC-27 cell proliferation
With the blank group than * p ﹤ 0.05, * * p ﹤ 0.01
Table 6 medicine under normal pH value (PH=7.5) on the impact of GES-1 cell proliferation
Experimental result shows, DABAI bolt ethanol extract has certain inhibitory action to the propagation of stomach cancer cell cell, its inhibitory action strengthens greatly under sour environment, low concentration 3-hydrogenation pine Siberian cocklebur acid B(10 μ g/ml, 5 μ g/ml), the suppression ratio to cell behind high concentration DABAI bolt ethanol extract (40 μ g/ml) and DABAI bolt water extract (40 μ g/ml) the effect 24h reaches respectively 61.4%, 81.5%, 40% and 32.9%, and this concentration does not almost affect people's normal gastric mucosa epithelial cell under normal pH environment.
4. anti esophageal cancer Experiment on Function
The In vitro culture of cell strain: human esophagus cancer cell strain Eca-109 is placed RPMI1640 culture fluid (containing 10% hyclone and penicillin, each 100U/ml of streptomycin), at 37 ℃ of saturated humidities, 5%CO
2Cultivate in the incubator.Treat that cell enters the logarithmic growth after date, through 0.25% trypsinization, collect, be deployed into corresponding cell concentration.
MTT test: be prepared into 1 * 10
4The Eca-109 cell suspension of/ml, add in 96 orifice plates after the piping and druming evenly, after cultivating 12h, be divided into blank group, positive group (20 μ g/ml mitomycin) and Trametes laclinea Berk ethanol extract, Trametes laclinea Berk water extract and B5 experimental group of 3-hydrogenation pine Siberian cocklebur acid, 4 dosage groups of each experiment component, the medicine (100 that adds respectively variable concentrations, 80,40,20 and 10 μ g/ml), matched group adds culture fluid, continues to cultivate 48h, then every hole adds MTT (5mg/ml) 20 μ l, continues to cultivate 4h.Discard culture fluid, every hole adds DMSO150 μ l, slightly shakes, and detects the trap (OD value) in every hole at wavelength 492nm place with enzyme-linked immunosorbent assay instrument.
Table 7 DABAI bolt extract is to the In-vitro Inhibitory Effect of Eca-109 cell
The result shows, DABAI bolt extract has inhibitory action to the propagation of esophageal carcinoma Eca-109 cell line.
5. Chinese People's Anti-Japanese Military and Political College's intestinal cancer Experiment on Function
The In vitro culture of cell strain: Human Large Intestine Carcinoma Cells strain LOVO is placed DMEM culture fluid (containing 10% hyclone and penicillin, each 100U/ml of streptomycin), 37 ℃ of saturated humidities, cultivate in the 5%CO2 incubator.Treat that cell enters the logarithmic growth after date, through 0.25% trypsinization, collect, be deployed into corresponding cell concentration.
MTT test: be prepared into 5 * 10
4The LOVO cell suspension of/ml adds after the piping and druming evenly in 96 orifice plates, behind the cultivation 12h, be divided into blank group, positive group (20 μ g/ml mitomycin) and Trametes laclinea Berk ethanol extract, Trametes laclinea Berk water extract and B3 experimental group of 3-hydrogenation pine Siberian cocklebur acid, 4 dosage groups of each experiment component add respectively the medicine (100,80 of variable concentrations, 40,20 and 10 μ g/ml), matched group adds culture fluid, continues to cultivate 48h, then every hole adds MTT (5mg/ml) 20 μ l, continues to cultivate 4h.Discard culture fluid, every hole adds DMSO150 μ l, slightly shakes, and detects the trap (OD value) in every hole at wavelength 492nm place with enzyme-linked immunosorbent assay instrument.
Table 7 DABAI bolt extract is to the In-vitro Inhibitory Effect of LOVO cell
The result shows, DABAI bolt extract has inhibitory action to the propagation of colorectal cancer LOVO cell.
Claims (9)
1. Trametes laclinea Berk silk, Trametes laclinea Berk extract or the application of 3-hydrogenation pine Siberian cocklebur acid B in the medicine of preparation prevention and treatment peptic ulcer.
2. Trametes laclinea Berk silk, Trametes laclinea Berk extract or the application of 3-hydrogenation pine Siberian cocklebur acid B in the medicine of preparation prevention and treatment non-ucler dyspepsia.
3. Trametes laclinea Berk silk, Trametes laclinea Berk extract or the application of 3-hydrogenation pine Siberian cocklebur acid B in the medicine of preparation prevention and treatment gastric cancer.
4. Trametes laclinea Berk silk, Trametes laclinea Berk extract or the application of 3-hydrogenation pine Siberian cocklebur acid B in the medicine of preparation prevention and the treatment esophageal carcinoma.
5. Trametes laclinea Berk silk, Trametes laclinea Berk extract or the application of 3-hydrogenation pine Siberian cocklebur acid B in the medicine of preparation prevention and treatment colorectal cancer.
6. the application in the medicine of peptic ulcer according to claim 1 is characterized in that: Trametes laclinea Berk silk, Trametes laclinea Berk extract or 3-hydrogenation pine Siberian cocklebur acid B have inhibition H
+, K
+-ATPase activity and anti-ulcer effect.
7. any one purposes is characterized in that: Trametes laclinea Berk silk and the sporophore of Trametes laclinea Berk filament for cultivating with corn culture medium according to claim 1-6.
8. any one purposes according to claim 1-6 is characterized in that: the Trametes laclinea Berk extract is water extract and with the ethanol extraction of 80-95%.
9. any one purposes according to claim 1-6 is characterized in that: the dosage parts by weight of 3-hydrogenation pine Siberian cocklebur acid B are 5-60 part.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510328957.3A CN104971069B (en) | 2013-05-14 | 2013-05-14 | A kind of applications of the 3 loose Siberian cocklebur acid B of hydrogenation extracted in great Bai bolts bacterium in pharmacy |
| CN201510328462.0A CN104940244B (en) | 2013-05-14 | 2013-05-14 | A kind of application of ethanol extract of great Bai bolts bacterium 95% in pharmacy |
| CN201510328463.5A CN104940245B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts bacterium water extract in pharmacy |
| CN201310176266.7A CN103316051B (en) | 2013-05-14 | 2013-05-14 | New functions and applications of trametes lactinea(Berk.)Pat. or extractives and trametenloic acid B |
| CN201510330206.5A CN104983750B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts mycelia in pharmacy |
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|---|---|---|---|
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| CN201510328463.5A Division CN104940245B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts bacterium water extract in pharmacy |
| CN201510330206.5A Division CN104983750B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts mycelia in pharmacy |
| CN201510328462.0A Division CN104940244B (en) | 2013-05-14 | 2013-05-14 | A kind of application of ethanol extract of great Bai bolts bacterium 95% in pharmacy |
| CN201510328957.3A Division CN104971069B (en) | 2013-05-14 | 2013-05-14 | A kind of applications of the 3 loose Siberian cocklebur acid B of hydrogenation extracted in great Bai bolts bacterium in pharmacy |
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| CN201510328463.5A Active CN104940245B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts bacterium water extract in pharmacy |
| CN201510328462.0A Active CN104940244B (en) | 2013-05-14 | 2013-05-14 | A kind of application of ethanol extract of great Bai bolts bacterium 95% in pharmacy |
| CN201510330206.5A Active CN104983750B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts mycelia in pharmacy |
| CN201510328957.3A Active CN104971069B (en) | 2013-05-14 | 2013-05-14 | A kind of applications of the 3 loose Siberian cocklebur acid B of hydrogenation extracted in great Bai bolts bacterium in pharmacy |
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| CN201510330206.5A Active CN104983750B (en) | 2013-05-14 | 2013-05-14 | A kind of application of great Bai bolts mycelia in pharmacy |
| CN201510328957.3A Active CN104971069B (en) | 2013-05-14 | 2013-05-14 | A kind of applications of the 3 loose Siberian cocklebur acid B of hydrogenation extracted in great Bai bolts bacterium in pharmacy |
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| CN113384591A (en) * | 2021-06-28 | 2021-09-14 | 三峡大学 | Combined drug of trametes acid and sorafenib and application of combined drug in preparation of antitumor drug |
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| CN105949262B (en) * | 2016-05-27 | 2017-05-24 | 三峡大学 | 3-hydrogenated pinicolic acid cyanide ethyl ester medicine and application thereof |
| CN110063960B (en) * | 2019-05-07 | 2021-05-18 | 三峡大学 | Pharmaceutical application of 3-hydrogenated pinolenic acid B cyanomethyl ester |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003171306A (en) * | 2001-12-04 | 2003-06-20 | Tadashi Goino | Physiologically active composition and method for producing the same |
| CN101245318A (en) * | 2008-02-28 | 2008-08-20 | 三峡大学 | Artificial cultivation method of Basidiomycetes and uses thereof |
| JP2009024027A (en) * | 2008-09-16 | 2009-02-05 | Tadashi Goino | Physiologically active composition |
| CN101439055A (en) * | 2007-11-19 | 2009-05-27 | 启东盖天力药业有限公司 | Use of aqueous extract of Trametes robiniophila Murr. in preparing medicament |
| CN101933950A (en) * | 2009-07-03 | 2011-01-05 | 启东盖天力药业有限公司 | Application of Trametes robiniophila Murr aqueous extract |
| CN102028716A (en) * | 2009-09-28 | 2011-04-27 | 郭志勇 | New application of basidiomycetes or extract thereof (3-trametenloic acid B) |
| TW201215396A (en) * | 2010-09-10 | 2012-04-16 | Kureha Corp | Medicine for treating tumor and antitumor agent |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5610415B2 (en) * | 2009-02-10 | 2014-10-22 | 国立大学法人鳥取大学 | Alcohol production method, alcoholic beverage production method, alcohol-containing food production method, and inoculum used therefor |
| CN103316051B (en) * | 2013-05-14 | 2015-07-22 | 三峡大学 | New functions and applications of trametes lactinea(Berk.)Pat. or extractives and trametenloic acid B |
-
2013
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003171306A (en) * | 2001-12-04 | 2003-06-20 | Tadashi Goino | Physiologically active composition and method for producing the same |
| CN101439055A (en) * | 2007-11-19 | 2009-05-27 | 启东盖天力药业有限公司 | Use of aqueous extract of Trametes robiniophila Murr. in preparing medicament |
| CN101245318A (en) * | 2008-02-28 | 2008-08-20 | 三峡大学 | Artificial cultivation method of Basidiomycetes and uses thereof |
| JP2009024027A (en) * | 2008-09-16 | 2009-02-05 | Tadashi Goino | Physiologically active composition |
| CN101933950A (en) * | 2009-07-03 | 2011-01-05 | 启东盖天力药业有限公司 | Application of Trametes robiniophila Murr aqueous extract |
| CN102028716A (en) * | 2009-09-28 | 2011-04-27 | 郭志勇 | New application of basidiomycetes or extract thereof (3-trametenloic acid B) |
| TW201215396A (en) * | 2010-09-10 | 2012-04-16 | Kureha Corp | Medicine for treating tumor and antitumor agent |
Non-Patent Citations (2)
| Title |
|---|
| 王星琴等: "《大白栓菌中32氢化松苓酸B含量的测定》", 《三峡大学学报(自然科学版)》 * |
| 王星琴等: "大白栓菌的生物学特征及成分检测", 《时珍国医国药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113384591A (en) * | 2021-06-28 | 2021-09-14 | 三峡大学 | Combined drug of trametes acid and sorafenib and application of combined drug in preparation of antitumor drug |
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|---|---|
| CN103316051B (en) | 2015-07-22 |
| CN104971069A (en) | 2015-10-14 |
| CN104940245A (en) | 2015-09-30 |
| CN104983750A (en) | 2015-10-21 |
| CN104983750B (en) | 2018-06-26 |
| CN104971069B (en) | 2017-12-19 |
| CN104940244B (en) | 2018-03-02 |
| CN104940244A (en) | 2015-09-30 |
| CN104940245B (en) | 2018-03-02 |
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