CN103300184B - Preparation method of efficient tea extract - Google Patents
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- CN103300184B CN103300184B CN201310209052.5A CN201310209052A CN103300184B CN 103300184 B CN103300184 B CN 103300184B CN 201310209052 A CN201310209052 A CN 201310209052A CN 103300184 B CN103300184 B CN 103300184B
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- 239000000284 extract Substances 0.000 title claims abstract description 17
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Abstract
The invention discloses a preparation method of an efficient tea extract. The preparation method of the efficient tea extract comprises the following steps of: grinding tea materials, leaching the ground materials with water at 60-90 DEG C for 5-30 minutes, then cooling to be 45-55 DEG C after the leaching is ended, regulating the pH value to be 4-6, adding degrading enzyme and reacting for 3-12 hours, rising the temperature to be 80-90 DEG C after reaction, carrying out thermal insulation at 80-90 DEG C for 10-15 minutes, and separating the degraded materials from water to obtain the tea extract. According to the preparation method, the material use ratio is increased, the production cost is reduced, the value of middle-and-low-grade tea materials is added by manufacture, and high-level utilization of tea resources is realized.
Description
Technical field
The present invention relates to a kind of tea-manufacturing technology, be specifically related to a kind of preparation method of efficient tea extraction.
Background technology
Tea is Chinese traditional drink, has deep cultural deposits, has plurality of health care functions simultaneously.China's tea beverage is since the starting nineties, at present in Rapid development stage.Rapid emergence and the development of tea beverage are to have advantages of based on tea itself.Raising along with people's living standard, consumer requires higher to aspects such as tea beverage taste, quality, safety and health cares, consumer to tea nutritional labeling and whether natural health, environmental protection and fashion grade etc. have the concern of higher psychological levels demand, to tea, also more and more higher expectation can be proposed.
The major issue facing when tea extracts is at present exactly that yield is low, causes efficiency low, and waste is large.It is reported that China's instant tea yield is only 18-25%, and abroad generally up to more than 30%.The diffusion that major reason is exactly active ingredient in tealeaves, leach that to be subject to obstruction or the Related Component utilization of cell membrane incomplete.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of preparation method of efficient tea extraction, reduce production costs, realize the value added by manufacture of centering low-grade tea raw material and the high level utilization of teas resource.
For achieving the above object, the technical solution adopted in the present invention is as follows:
A preparation method for efficient tea extract, it is realized by following steps in turn:
1) tea raw material pulverization process;
2) raw material after pulverizing is first used flooding 5-30min, extraction temperature 60-90 ℃;
3) be cooled to 45-55 ℃ after finishing, regulate pH value 4-6, add digestive enzyme reaction 3-12h; After reaction finishes, rising temperature is to 80-90 ℃ of insulation 10-15min left and right;
4) raw material and aqueous dispersion after degraded are separated, obtain tea extraction.
The present invention can also realize in the following manner:
As a kind of preferred scheme of the present invention, in said method, when adding digestive enzyme in step 3), add enzymatic protective reagent.That in the present invention, adds enzymatic protective reagent can remove the disturbing factor to enzymatic activity; significantly improve enzymatic activity and stability; some key issues from solve in essence tealeaves deep-processing process; reduce production costs, realize the value added by manufacture of centering low-grade tea raw material and the high level utilization of teas resource.
Preferred enzymatic protective reagent is a kind of in glucose, mannose, fructose, sucrose and shitosan, trehalose in the present invention; or a kind of in propane diols, glycerine and sorbierite, or a kind of in macroporous absorbent resin, silica gel or PVPP, protein, starch.The addition of described enzymatic protective reagent is 5 ~ 50mg/L.Wherein preferably enzymatic protective reagent is PVPP, and PVPP enzymatic protective reagent can also be reused after using and rinsing regeneration by washing, alkali cleaning, pickling and water; The addition of applying disposable PVPP is 5 ~ 50mg/L, and the addition of application regeneration PVPP is 20 ~ 100mg/L.
In said method, the consumption of described digestive enzyme is to add 0.002-0.02g in every g tea raw material.In the specific embodiment of the invention, the digestive enzyme adopting is a kind of or two or more with arbitrarily than mixing in cellulase, hemicellulase or pectase.
In order to reach good extracting effect, in said method, step 2) in, the mass ratio of tea raw material and water is 1:(5-15).
In the present invention, after enzyme is processed, teas raw material becomes muddy completely, adopts horizontal centrifugal or belt expression separation tea juice and tea grounds in step 4).In the process of separated tea juice and tea grounds, add water tea grounds is rinsed.
Tea raw material of the present invention can be any in green tea, black tea, oolong tea or other teas, also can be the plants herb raw material of cold tea class.
Preparation method of the present invention also comprised to the extract in step 4) further concentrated be processed into inspissated juice or dry after be processed into instant powder.
Compared to existing technology, beneficial effect of the present invention is:
1. the preparation method of efficient tea extraction of the present invention at least improves more than 30% than utilization rate under equal conditions or under the existing large condition of production;
2. the preparation method of efficient tea extraction of the present invention can realize effective conversion of raw materials used 25%-75%, the unserviceable macromolecular substances such as the cellulose in teas raw tissue structure, hemicellulose especially, and its availability significantly improves;
3. the preparation method of efficient tea extraction of the present invention degrades to teas raw tissue structure by adding digestive enzyme, be conducive to the release of endogenous thing or approaching of enzyme-to-substrate, impel the degree of depth of reaction to carry out, make the teas extract inclusion for preparing abundanter; And enzyme is processed the reduced viscosity of extraction slurries afterwards, it is loose that institutional framework becomes, and is conducive to realize the separated of tea grounds and liquid dispersion phase in slurries, is conducive to improve follow-up process, as shorten the operating process times such as separation, filtration, enhance productivity;
4. the preparation method of efficient tea extraction of the present invention significantly reduces the discharge of tea grounds the like waste, realizes in relatively gentle condition and being issued to the efficient object of utilizing of teas raw material, extracts the loss impact on teas local flavor and flavour while avoiding high temperature long.
Below in conjunction with the specific embodiment, the present invention is described in further detail.
The specific embodiment
Embodiment 1
Take 4 portions of 100g tealeaves, press respectively 70 ℃ of extraction 10min of 1:8 tea-water proportion, extraction finishes rear adjustment temperature to 50 ℃ left and right, adds respectively the PVPP of 0.2g and the dissimilar cellulase of 0.2g to compare (specifically in Table 1), is uniformly mixed reaction 6h, after reaction finishes, remove by filter tea grounds and obtain tea extract, quickly heat up to 90 ℃, after insulation 10min, be cooled to rapidly 30 ℃, then through centrifugal, after reduced pressure concentration, make tea extraction, comparative result is in Table 1.
Table 1
| Numbering | Digestive enzyme | Similar comparison on market | Yield (%) |
| a | Cellulase | / | 33.27 |
| b | Cellulase | Novozymes (Novi's letter) | 26.80 |
| c | Cellulase | Amano Enzyme (day open country) | 23.03 |
| d | Cellulase | Amano Enzyme (day open country) | 21.35 |
Embodiment 2
Take 4 portions of 100g tealeaves, press respectively 70 ℃ of extraction 10min of 1:8 tea-water proportion, extraction finishes rear adjustment temperature to 50 ℃ left and right, add respectively the PVPP of 0.2g and the dissimilar pectase of 0.2g to compare (specifically in Table 2), be uniformly mixed reaction 6h, after reaction finishes, remove by filter the extract that tea grounds obtains, after quickly heating up to 90 ℃ of insulation 10min, be cooled to rapidly 30 ℃, through centrifugal, make tea extraction after reduced pressure concentration again, comparative result is in Table 2.
Table 2
| Numbering | Digestive enzyme | Similar comparison on market | Yield (%) |
| a | Pectase | / | 12.13 |
| b | Pectase | Novozymes (Novi's letter) | 9.12 |
| c | Pectase | Novozymes (Novi's letter) | 8.05 |
| d | Pectase | Amano Enzyme (day open country) | 9.47 |
Embodiment 3
Take 3 portions of 100g tealeaves, press respectively 70 ℃ of extraction 10min of 1:8 tea-water proportion, extraction finishes rear adjustment temperature to 50 ℃ left and right, adds respectively the combination of the PVPP of 0.2g and the dissimilar cellulase of 0.2g and pectase to compare (specifically in Table 3), is uniformly mixed reaction 6hr.Reaction removes by filter the tea extract that tea grounds obtains after finishing, and is cooled to rapidly 30 ℃ after quickly heating up to 90 ℃ of insulation 10min, then through centrifugal, makes tea extraction after reduced pressure concentration, and comparative result is in Table 3.
Table 3
| Numbering | Enzyme combination used | Similar comparison on market | Yield (%) |
| a | Cellulase, pectase | / | 45.44 |
| b | Cellulase, pectase | Novozymes (Novi's letter) | 32.07 |
| c | Cellulase, pectase | Amano Enzyme (day open country) | 33.41 |
Embodiment 4
Take 3 portions of 100g tealeaves, press respectively 70 ℃ of extraction 10min of 1:8 tea-water proportion, extraction finishes rear adjustment temperature to 50 ℃ left and right, add respectively the combination of different cellulases, hemicellulase and pectase of PVPP, each 0.2g of 0.2g to compare (specifically in Table 4), be uniformly mixed reaction 6hr.Reaction removes by filter the extract that tea grounds obtains after finishing, and is cooled to rapidly 30 ℃ after quickly heating up to 90 ℃ of insulation 10min, then through centrifugal, makes tea extraction after reduced pressure concentration, and comparative result is in Table 4.
Table 4
| Numbering | Digestive enzyme | Yield (%) |
| a | Cellulase, hemicellulase | 40.03 |
| b | Cellulase, pectase | 45.44 |
| c | Cellulase, hemicellulase, pectase | 52.17 |
Comparative example 1
Take 2 portions of 100g tealeaves; press respectively 70 ℃ of extraction 10min of 1:8 tea-water proportion; extraction finishes rear adjustment temperature to 50 ℃ left and right; a copy of it adds the PVPP of 0.2g; add respectively again the enzyme combination of cellulase, hemicellulase and the pectase of 0.2g (to adding and not adding enzymatic protective reagent, to compare; in Table 5), be uniformly mixed reaction 6hr.Reaction removes by filter the extract that tea grounds obtains after finishing, and is cooled to rapidly 30 ℃ after quickly heating up to 90 ℃ of insulation 10min, then through centrifugal, makes tea extraction after reduced pressure concentration, and comparative result is in Table 5.
Table 5
| Numbering | Digestive enzyme | Protective agent | Yield (%) |
| a | Cellulase, hemicellulase, pectase | Do not add | 46.18 |
| b | Cellulase, hemicellulase, pectase | Add | 52.17 |
Result of implementation is analyzed
From embodiment 1 and example 2, can find out, the enzyme effect difference of separate sources is very large, and obvious advantage appears in several enzyme performances of the selection of this method.
Outside the cellulase using in embodiment 1, further use the embodiment 3 of pectase and use in the embodiment 4 of enzyme hemicellulase, pectase, extract yield further increases, and difference is also more obvious.
From comparative example 1, can find out, after interpolation enzymatic protective reagent, significantly improve enzymatic activity and stability.
The present invention is improving tea raw material in efficient utilization, to increase tea pure local flavor, to improve flavour mouthfeel also helpful, and a kind of method of preparing high-quality tea extraction is also provided simultaneously.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, the variation of any unsubstantiality that those skilled in the art does on basis of the present invention and replacement all belong to the present invention's scope required for protection.
Claims (6)
1. a preparation method for efficient tea extraction, is characterized in that it realizes by following steps in turn:
1) tea raw material pulverization process;
2) raw material after pulverizing is first used flooding 5-30min, extraction temperature 60-90 ℃;
3) be cooled to 45-55 ℃ after finishing, regulate pH value 4-6, add digestive enzyme and enzymatic protective reagent PVPP reaction 3-12h; After reaction finishes, rising temperature is to 80-90 ℃ of insulation 10-15min; Wherein, the consumption of digestive enzyme is to add 0.002-0.02g in every g tea raw material; Described digestive enzyme is two or more with arbitrarily than mixing in cellulase, hemicellulase and pectase;
4) by raw material and aqueous phase separation after degraded, obtain tea extract.
2. the preparation method of efficient tea extraction according to claim 1, is characterized in that: the addition of described enzymatic protective reagent is 5～50mg/L.
3. the preparation method of efficient tea extraction according to claim 1, is characterized in that: it also comprises step 4) in tea extract be processed into the step of inspissated juice or instant powder.
4. the preparation method of efficient tea extraction according to claim 1, is characterized in that: step 4) middle horizontal centrifugal or belt expression separation tea juice and the tea grounds of adopting.
5. the preparation method of efficient tea extraction according to claim 4, is characterized in that: in the process of separated tea juice and tea grounds, add water tea grounds is rinsed.
6. the preparation method of efficient tea extraction according to claim 1, is characterized in that: described tea raw material is a kind of in the plants herb raw material of green tea, black tea, oolong tea, cold tea class.
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| CN103749798A (en) * | 2014-01-03 | 2014-04-30 | 广东省农业科学院饮用植物研究所 | Method for extracting EGCG (epigallocatechin gallate) in tea leaves by water extraction |
| CN105010617B (en) * | 2014-04-29 | 2020-02-11 | 统一企业(中国)投资有限公司昆山研究开发中心 | Instant black tea, ice black tea beverage and preparation method thereof |
| CN108522738A (en) * | 2015-06-17 | 2018-09-14 | 集美大学 | A method of increasing tea breast in millet paste using papain and is formed |
| CN105379876A (en) * | 2015-12-22 | 2016-03-09 | 四川藏匠茶业股份有限公司 | Health care Tibetan tea and preparation method thereof |
| CN105901249A (en) * | 2016-06-27 | 2016-08-31 | 怀宁大别山茶酒有限公司 | Hesperidium-scented tea beverage capable of beautifying and clearing heat and preparation method thereof |
| CN105941723A (en) * | 2016-06-27 | 2016-09-21 | 怀宁大别山茶酒有限公司 | Inflammation-diminishing and heat-clearing bergamot fruit flower scented tea drink and preparation method thereof |
| CN108771961B (en) * | 2018-07-03 | 2021-05-04 | 珠海华泽生物科技有限公司 | Biological enzyme formaldehyde removing agent and application thereof |
| CN111317047A (en) * | 2020-04-15 | 2020-06-23 | 西安艾默农林生物技术有限公司 | Preparation method of samara tea beverage |
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