CN103289998A - Molecular marker of pure white Hypsizygus marmoreus Finc-W-247, its acquisition method and application - Google Patents
Molecular marker of pure white Hypsizygus marmoreus Finc-W-247, its acquisition method and application Download PDFInfo
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Abstract
The invention relates to a molecular marker of a pure white Hypsizygus marmoreus Finc-W-247. Its specific PCR amplification primer pair sequences are 5'-GACGACGTTTGTGGGGTGA-3' and 5'-AGGGAGATCGACGTCCATATTG-3', and the size of its DNA fragment is 975bp. The invention also discloses an acquisition method and application of the molecular marker of the pure white Hypsizygus marmoreus Finc-W-247.
Description
[technical field]
The invention belongs to microbial technology field, be specifically related to molecule marker and preparation method and the application of a kind of pure white Hypsizygus marmoreus Finc-W-247.
[background technology]
Hypsizygus marmoreus (Latin title Hypsizygus marmoreus) is subordinate to Basidiomycotina, Hymenomycetes, Agaricales, white mushroom section, beautiful gill fungus genus, because it has unique crab delicate flavour, so claim that again it is crab mushroom.Initial artificial culture started from Japan before and after 1978, its delicious flavour, sense of food are tender and crisp, nutritious, and it is distinguished the flavor of than flat mushroom aquatic foods, and its meat is thicker than sliding mushroom, and its matter is more tough than mushroom, and mouthfeel is excellent, and its dry product also thick flavor overflows.Hypsizygus marmoreus contains abundant VITAMIN and 17 seed amino acids, and the beta-glucan that extracts in the sporophore has very high anti-tumor activity, is a kind of protective foods low in calories, low-fat.
The discriminating of tradition edible fungus species, especially for Hypsizygus marmoreus, the mode of appearance that its main foundation is sporophore, antagonistic effect, fruiting contrast etc.Mode of appearance is subject to the influence of planting environment and prescription, the difficulty that causes mode of appearance to identify.Between the nearer bacterial strain of sibship (such as, between some parent and the filial generation), antagonism is not obvious, differentiates that by antagonistic effect bacterial classification also has difficulties.At least 4 months consuming time of fruiting simultaneous test is unfavorable for evaluation and the detection of bacterial classification.Along with development of molecular biology, molecular labeling method is used for the discriminating of bacterial classification more and more widely.SCAR(Sequence-characterized Amplified Region) mark is a kind of very stable molecule marker, can be easy, quick, stable carry out strain identification, but at present pair SCAR molecule marker of pure white Hypsizygus marmoreus Finc-W-247 is not arranged as yet, with Rapid identification pure white Hypsizygus marmoreus Finc-W-247.
[summary of the invention]
The technical problem that the present invention solves is to overcome the defective that prior art exists, and molecule marker and preparation method and the application of a kind of pure white Hypsizygus marmoreus Finc-W-247 are provided.
The present invention adopts round pcr to carry out a large amount of shaker tests, wherein, (sequence is: the specific DNA fragment that GTCCCGACGA) has obtained Hypsizygus marmoreus Finc-W-247 bacterial strain or its sporophore to adopt random primer, molecular weight is 989bp, with this fragment cloning order-checking, based on the dna sequence dna of this fragment, design specific PCR amplimer, its special primer to sequence is: 5 '-GACGACGTTTGTGGGGTGA-3' and 5 '-AGGGAGATCGACGTCCATATTG-3'.This primer can obtain the molecular weight size and be the specific fragment of 975bp Hypsizygus marmoreus Finc-W-247 bacterial strain or its sporophore are carried out the SCAR-PCR amplification, is the molecule marker of pure white Hypsizygus marmoreus bacterial strain Finc-W-247.
The present invention also discloses the preparation method of the molecule marker of a kind of pure white Hypsizygus marmoreus Finc-W-247, it is characterized in that comprising the steps:
1) obtains mycelia or sporophore;
2) extracting genome DNA;
3) specific DNA fragment is analyzed and obtained to the RAPD method: adopt the analysis of RAPD method, obtain the specific DNA fragment of pure white Hypsizygus marmoreus Finc-W-247, the random primer sequence that obtains this fragment correspondence is: GTCCCGACGA;
4) special primer design: based on the specific DNA fragment sequence of above-mentioned acquisition, design specific PCR amplimer, primer is 5'-GACGACGTTTGTGGGGTGA-3' and 5'-AGGGAGATCGACGTCCATATTG-3' to sequence;
5) pcr amplification of SCAR molecule marker: with the specific PCR amplimer genome of pure white Hypsizygus marmoreus Finc-W-247 is carried out the SCAR-PCR amplification, obtain the specific molecular marker of 975bp.
6) electrophoresis detection.
The pcr amplification reaction system of described step 3) RAPD method is: cumulative volume 25 μ L: genomic templates 20-30ng/ μ L1.0 μ L, random primer 0.5pmol/ μ L1.0 μ L, 10mM dNTP mix0.5 μ L, 10 * Taq damping fluid, 2.5 μ L, 25mM MgCl
22.0 μ L, Taq enzyme 5U/ μ L0.25 μ L adds water to 25 μ L.PCR reaction conditions: pre-95 ℃ of 3min of sex change; 94 ℃ of 45s, 36 ℃ of 60s, 72 ℃ of 2min, 35 circulations; 72 ℃ are extended 10min.Pure white Hypsizygus marmoreus Finc-W-247 bacterial strain or its sporophore specific DNA fragment size are 989bp.
The pcr amplification of SCAR molecule marker in the described step 5), its system is: cumulative volume 25 μ L, template 20～30ng/ μ L1 μ L, Hypsizygus marmoreus Finc-W-247 special primer is to each 0.5 μ L of 0.5pmol/ μ L, 10mM dNTP mix0.5 μ L, 10 * Taq Buffer2.5 μ L, 25mM MgCl
22.0 μ L, Taq enzyme 5U/ μ L0.25 μ L adds water to 25 μ L.PCR reaction conditions: pre-95 ℃ of 3min of sex change; 94 ℃ of 30s, 62 ℃ of 30s, 72 ℃ of 1min, 35 circulations; 72 ℃ are extended 5min.
The molecule marker that the present invention discloses a kind of pure white Hypsizygus marmoreus Finc-W-247 again is used for the Rapid identification of pure white Hypsizygus marmoreus Finc-W-247 bacterial strain and the application of detection; Be specially and adopt pure white Hypsizygus marmoreus Finc-W-247 bacterial strain special primer that pure white Hypsizygus marmoreus bacterial strain is carried out pcr amplification, according to the dna fragmentation that can produce the 975bp size, as the foundation that pure white Hypsizygus marmoreus Finc-W-247 bacterial strain is identified and detected.
Above-mentioned detection method is compared with authentication methods such as the morphologic detection of routine, antagonistic effect and the contrasts of cultivation fruiting, has short, advantage of high accuracy detection time.It can overcome morphologic detection, the antagonistic effect tolerance range is poor, can overcome the shortcoming that the cultivation fruiting contrasts length consuming time again.Present method finds that it has the specificity of Finc-W-247 bacterial strain or its sporophore in being applied to existing white Hypsizygus marmoreus bacterial strain detection.
[description of drawings]
Fig. 1 be random primer S67 to the electrophoretogram of the pcr amplification product of 4 pure white Hypsizygus marmoreus bacterial strains, wherein 1 is H-W, 2 is Finc-W-247,3 is G-W, 4 is GC-W, M is 10kbp DNA ladder.
Fig. 2 is special primer to the electrophoretogram to the pcr amplification product of 4 pure white Hypsizygus marmoreus bacterial strains, and wherein 1 is H-W, and 2 is Finc-W-247, and 3 is G-W, and 4 is GC-W, and M is 10kbp DNA ladder.
[embodiment]
Below in conjunction with specific embodiment the present invention is described in further detail.
" H-W " is the pure white Hypsizygus marmoreus kind that Japanese Big Dipper Co., Ltd. in 2002 seed selection is released in the present embodiment, snow-white, the no bitter taste of its entire body; " G-W " bacterial strain is a kind of Hypsizygus marmoreus bacterial strain of buying from the market, and its original hase is grey, and along with the g and D of sporophore bleaches gradually, collection period, stem was canescence, and cap is pure white; " GC-W " bacterial strain is the white jade mushroom bacterial strain that Japanese Ge Cheng newly breeds.
The preparation method of the molecule marker of a kind of pure white Hypsizygus marmoreus Finc-W-247 comprises the steps:
1) strain culturing and aerial hyphae obtain
The Finc-W-247 of cryopreservation and H-W, G-W and GC-W are inoculated on the PDA substratum, cultivated 20 days under the condition of 22 ℃ of temperature, humidity 75%, scrape and get aerial hyphae ,-20 ℃ frozen.
2) extracting genome DNA
In the present embodiment, carry out SK1375 fungal gene group DNA extraction agent box specification sheets extraction strain gene group according to giving birth to worker's biotechnology (Shanghai) limited-liability company (hereinafter to be referred as " giving birth to the worker "), resulting dna solution is placed-20 ℃ of refrigerations, standby.
3) the RAPD method is analyzed
Selecting for use 16 random primers (seeing Table 1) to carry out RAPD analyzes.The pcr amplification system is: cumulative volume 25 μ L; Genomic templates 20-30ng/ μ L1.0 μ L, random primer 0.5pmol/ μ L1.0 μ L, 10mM dNTP mix0.5 μ L, 10 * Taq damping fluid, 2.5 μ L, 25mM MgCl
22.0 μ L, Taq enzyme 5U/ μ L0.25 μ L adds water to 25 μ L.PCR reaction conditions: pre-95 ℃ of 3mim of sex change; 94 ℃ of 45s, 36 ℃ of 60s, 72 ℃ of 2min, 35 circulations; 72 ℃ are extended 10min.
Table 1 random primer
Through screening, random primer S67 can increase and obtain the peculiar DNA band of Finc-W-247 bacterial strain, sees also Fig. 1.As can be seen from Figure 1, the band of a frame position is peculiar by Finc-W-247, does not occur and all there is this band on H-W, G-W and the GC-W place swimming lane correspondence position.
The distinctive DNA band of a frame position Finc-W-247 in the cutting drawing 1 reclaims DNA.Recovery method reclaims the test kit explanation according to giving birth to worker UNIQ-10 pillar DNA glue.The DNA that reclaims adopts conventional sequence measurement, the results are shown in sequence table, and it is the dna fragmentation of 989bp.
4) acquisition of special primer
Dna fragmentation two ends base sequence according to above-mentioned 989bp, design special primer sequence, several bases are respectively given up at described dna fragmentation two ends, and the amplimer of final design is 5 '-GACGACGTTTGTGGGGTGA-3' and 5 '-AGGGAGATCGACGTCCATATTG-3' to sequence.Adopt the above-mentioned special primer sequence of Primer premier5.0 software design in the present embodiment.
5) pcr amplification of SCAR molecule marker
With the specific PCR amplimer Finc-W-247 and H-W, G-W and GC-W are carried out the SCAR-PCR amplification, its amplification system is: cumulative volume 25 μ L: template 20～30ng/ μ L1 μ L, Hypsizygus marmoreus Finc-W-247 special primer is to each 0.5 μ L of 0.5pmol/ μ L, 10mM dNTP mix0.5 μ L, 10 * Taq Buffer2.5 μ L, 25mM MgCl
22.0 μ L, Taq enzyme 5U/ μ L0.25 μ L adds water to 25 μ L.PCR reaction conditions: pre-95 ℃ of 3min of sex change; 94 ℃ of 30s, 62 ℃ of 30s, 72 ℃ 1min35 circulation; 72 ℃ are extended 5min.
6) electrophoresis detection
Get above-mentioned pcr amplification product 6 μ L, with 1 μ L sample loading buffer mixing, point sample is on 1.5% sepharose, in 0.5 * tbe buffer liquid, electrophoresis under the 5V/cm voltage is after electrophoresis finishes, dye with EB, carry out agarose gel electrophoresis, take a picture at the gel imaging instrument then, its result sees also Fig. 2 electrophoretogram.
As can be seen from Figure 2, have only the new bacterial strain Finc-W-247 of pure white Hypsizygus marmoreus of the present invention bacterial strain that single-minded amplified band is arranged, and other three kinds existing pure white Hypsizygus marmoreus bacterial strain H-W, G-W and GC-W all do not have amplified band, illustrate that 5'-GACGACGTTTGTGGGGTGA-3' and 5 '-AGGGAGATCGACGTCCATATTG-3' is the SCAR molecule marker primer of Finc-W-247.Be 975bp with this primer to the dna fragmentation molecular weight that amplifies, the specific DNA fragment of described 975bp is the SCAR molecule marker of the new bacterial strain Finc-W-247 of Hypsizygus marmoreus of the present invention.
In the present embodiment, adopt mycelia to carry out the extraction of genomic dna, obviously, adopt sporophore can carry out the extraction of genomic dna too.The method of extracting has been that prior art is disclosed, is not giving unnecessary details at this.
More than describing is embodiments of the invention only, forgives and can understand, and under the prerequisite that does not depart from the present invention's design, all should be included within the technical conceive of the present invention simple modification of the present invention and replacement.
Sequence table
Sequence
Table
＜110〉Shanghai Finc Bio-tech Inc.
＜120〉Finc-w-247 specific band
＜130〉the new bacterial strain Finc-w-247 of pure white Hypsizygus marmoreus
<160>1
<170>PatentIn version3.3
<210>1
<211>989
<212>DNA
<213>Hypsizygus marmoreus
<400>1
gtcccgacga cgtttgtggg gtgaacgatc ccgaattgca ggatcccctc ctccaatcac 60
aatatgaagg cttgccattt ctgccgtgag tcgacctgtt tgggaaaaat cagaaagcgc 120
cactcatctt catcgttcac ctttctttta tagcggcaaa tgttccatct acgggtggag 180
caccaagcag agcatcgtac ctgggtggac gatgttctct aagtggggga agcaaaaccc 240
aaatttgaat gtggggtatg tcgctgaggc tattaatgta aagcaatcct gacgctttcc 300
aggctcgcgg ttcagagctt gaaattcgtt cgatcggggc gttatgtcaa tcccagccga 360
attgcacccc ttcatctctc aactcgagtt gtttacggta gcgccgacac caggcgcctt 420
cataccagtg gaggctctgc catatgcgtc tctaccatct tctccgcaga gtctcttctc 480
gtcaacggca gagagcaggg tatggcgcaa aaacttctct ccggcatatt tcacagtcaa 540
gagtggcagc gcttcgaagc tatgatgtgc ttggctttta ctcaccgccg attgaaagcg 600
cagtacaaca atcatgcttt ggacttttgc acaaggccca gaccccaagg aaagtctccc 660
gccctttgct atacgctttt ttctttccgc ggttgttcaa aagcagtggc ttaacctgtt 720
ctttccatag atgccaccag ctcctccgtc ggttccaagt tcagcgctgc cgacggaatg 780
ttcggggcgg cgcccgccaa aggaaaagcg gagtccagtc acgggcactc ccctgttaca 840
aacaagcctg tcctggattt tgacgatgaa ggtcacttcc cctttcatga tctgcgtaga 900
atcatactca tccatgttcg tttccagtac ctgtctttga tgggcgtaac accaccatca 960
atatggacgt cgatctccct cgtcgggac 989
＜110〉Shanghai Finc Bio-tech Inc.
＜120〉molecule marker primer
＜130〉Hypsizygus marmoreus Finc-w-247 bacterial strain molecule marker
<160>2
<170>PatentIn version3.3
<210>1
<211>19
<212>DNA
＜213〉artificial sequence
<400>1
gacgacgttt gtggggtga 19
＜110〉Shanghai Finc Bio-tech Inc.
＜120〉molecule marker primer
＜130〉Hypsizygus marmoreus Finc-w-247 bacterial strain molecule marker
<160>3
<170>PatentIn version3.3
<210>1
<211>22
<212>DNA
＜213〉artificial sequence
<400>1
agggagatcg acgtccatat tg 22
Claims (5)
1. the molecule marker of a pure white Hypsizygus marmoreus Finc-W-247, it is characterized in that: the specific PCR amplimer is 5 '-GACGACGTTTGTGGGGTGA-3' and 5'-AGGGAGATCGACGTCCATATTG-3' to sequence, the dna fragmentation size is 975bp.
2. the preparation method of the molecule marker of a pure white Hypsizygus marmoreus Finc-W-247 is characterized in that comprising the steps:
1) obtains mycelia or sporophore;
2) extracting genome DNA;
3) specific DNA fragment is analyzed and obtained to the RAPD method: adopt the analysis of RAPD method, obtain the specific DNA fragment of pure white Hypsizygus marmoreus Finc-W-247, the random primer sequence that obtains this fragment correspondence is: GTCCCGACGA;
4) special primer design: based on the specific DNA fragment sequence of above-mentioned acquisition, design specific PCR amplimer, primer is 5'-GACGACGTTTGTGGGGTGA-3' and 5'-AGGGAGATCGACGTCCATATTG-3' to sequence;
5) pcr amplification of SCAR molecule marker: with the specific PCR amplimer genome of pure white Hypsizygus marmoreus Finc-W-247 is carried out the SCAR-PCR amplification, obtain the specific molecular marker of 975bp.
6) electrophoresis detection.
3. the preparation method of the molecule marker of a kind of pure white Hypsizygus marmoreus Finc-W-247 according to claim 2, it is characterized in that: the pcr amplification reaction system of described step 3) RAPD method is: cumulative volume 25 μ L: genomic templates 20-30ng/ μ L1.0 μ L, random primer 0.5pmol/ μ L1.0 μ L, 10mM dNTP mix0.5 μ L, 10 * Taq damping fluid, 2.5 μ L, 25mM MgCl
22.0 μ L, Taq enzyme 5U/ μ L0.25 μ L adds water to 25 μ L; PCR reaction conditions: pre-95 ℃ of 3min of sex change; 94 ℃ of 45s, 36 ℃ of 60s, 72 ℃ of 2min, 35 circulations; 72 ℃ are extended 10min; Pure white Hypsizygus marmoreus Finc-W-247 bacterial strain or its sporophore specific DNA fragment size are 989bp.
4. according to the preparation method of the molecule marker of claim 2 or 3 described a kind of pure white Hypsizygus marmoreus Finc-W-247, it is characterized in that: the pcr amplification of SCAR molecule marker in the described step 5), its system is: cumulative volume 25 μ L, template 20～30ng/ μ L1 μ L, the Finc-W-247 special primer is to each 0.5 μ L of 0.5pmol/ μ L, 10mM dNTP mix0.5 μ L, 10 * Taq Buffer2.5 μ L, 25mM MgCl
22.0 μ L, Taq enzyme 5U/ μ L0.25 μ L adds water to 25 μ L; PCR reaction conditions: pre-95 ℃ of 3min of sex change; 94 ℃ of 30s, 62 ℃ of 30s, 72 ℃ of 1min, 35 circulations; 72 ℃ are extended 5min.
5. the molecule marker of a kind of pure white Hypsizygus marmoreus Finc-W-247 bacterial strain according to claim 1 is applied to Rapid identification and the detection of pure white Hypsizygus marmoreus Finc-W-247 bacterial strain.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434381A (en) * | 2016-10-31 | 2017-02-22 | 上海丰科生物科技股份有限公司 | Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof |
| CN106434382A (en) * | 2016-10-31 | 2017-02-22 | 上海丰科生物科技股份有限公司 | Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684487A (en) * | 2008-09-24 | 2010-03-31 | 上海市农业科学院 | Method for identifying industrially cultivated strains of hypsizygus marmoreus by using SSR molecular marker |
-
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684487A (en) * | 2008-09-24 | 2010-03-31 | 上海市农业科学院 | Method for identifying industrially cultivated strains of hypsizygus marmoreus by using SSR molecular marker |
Non-Patent Citations (2)
| Title |
|---|
| 李挺 等: "真姬菇子实体ITS序列和RAPD分析", 《贵州农业科学》 * |
| 黄龙花 等: "基于特定引物PCR的DNA分子标记技术研究进展", 《生物技术通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434381A (en) * | 2016-10-31 | 2017-02-22 | 上海丰科生物科技股份有限公司 | Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof |
| CN106434382A (en) * | 2016-10-31 | 2017-02-22 | 上海丰科生物科技股份有限公司 | Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof |
| CN106434382B (en) * | 2016-10-31 | 2018-12-21 | 上海丰科生物科技股份有限公司 | Pure white true pleurotus cornucopiae bacterial strain and its molecular labeling, specific primer to and application |
| CN106434381B (en) * | 2016-10-31 | 2018-12-21 | 上海丰科生物科技股份有限公司 | Pure white true pleurotus cornucopiae bacterial strain and its molecular labeling, specific primer to and application |
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