CN103284159A - Linoleic acid loaded soy isolate protein/chitosan composite microcapsule and preparation method thereof - Google Patents
Linoleic acid loaded soy isolate protein/chitosan composite microcapsule and preparation method thereof Download PDFInfo
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- CN103284159A CN103284159A CN201310260475XA CN201310260475A CN103284159A CN 103284159 A CN103284159 A CN 103284159A CN 201310260475X A CN201310260475X A CN 201310260475XA CN 201310260475 A CN201310260475 A CN 201310260475A CN 103284159 A CN103284159 A CN 103284159A
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Abstract
The invention discloses a linoleic acid loaded soy isolate protein/chitosan composite microcapsule and a preparation method thereof, and belongs to the technical field of microcapsules of agricultural and sideline products. The preparation method comprises the steps of adding linoleic acid and a little of emulsifier to soy isolate protein solution to be mixed with acetum of chitosan, carrying out homogenization on the mixed solution for 10-15 minutes, so as to form uniform lactescence; adjusting the pH of the lactescence to the range of 4.5-4.8; magnetically stirring for 10-15 minutes; primarily forming the suspension solution of the microcapsule; adjusting the pH value of the suspension solution of the microcapsule to 7-7.3; adding a little of glycerol aqueous solution as a curing agent; magnetically stirring and curing for 1-2 hours; layering and extracting after stewing for 1-3 hours; collecting a web bag after washing by distilled water; freezing and drying in vacuum to obtain the linoleic acid loaded soy isolate protein/chitosan composite microcapsule. The embedding efficiency of the obtained microcapsule is over 60%; the embedding yield is over 65%; the composite microcapsule is long in shelf life and convenient to store.
Description
Technical field
The invention belongs to the microcapsules technology field, be specifically related to a kind of year linoleic soybean protein isolate/shitosan composite micro-capsule and preparation method thereof.
Background technology
Linoleic acid is that human body can not synthesize, or the aliphatic acid that far can not satisfy the demand of synthetic amount, is called essential fatty acid.Linoleic acid is a kind of essential fatty acid of generally acknowledging.Extremely pay attention to because linoleic acid can reduce blood cholesterol levels, and prevention of arterial is atherosis.Discover that cholesterol must could turn round and metabolism in vivo normally after linoleic acid is combined.If the shortage linoleic acid, cholesterol will be combined with some saturated fatty acids, and dysbolism takes place, and under the vascular wall deposition, progressively forms atherosclerotic, causes cardiovascular and cerebrovascular disease.Therefore, medically linoleic application is caused enough attention, be usually used in treating diseases such as the too high and artery sclerosis of blood fat.Some vegetable oil linoleic acid contents are higher, are 26% as peanut oil, and soya-bean oil is 57.5%, and rape oil is 15.8%.Yet autoxidation very easily takes place in linoleic acid in air, cause losing its effect, and this has brought a difficult problem just for linoleic preservation, need find a kind of better protect technology, promotes its stability.
Microcapsules technology refers to dispersed solids material, drop or gas are encapsulated in the method that forms microcapsules in one deck dense film fully.Usually dense film is to be made by natural or artificial synthetic macromolecular material.Microcapsules technology has been applied in the industry such as medicine, agricultural, computer, chemicals, food, cosmetics, has caused the extensive concern of academia.
Summary of the invention
The purpose of this invention is to provide a kind of year linoleic soybean protein isolate/shitosan composite micro-capsule, resulting compound microcapsules are made up of soybean protein isolate, shitosan, linoleic acid and emulsifying agent, resulting microscapsule powder exquisiteness, more than 80 orders, and embedding efficiency is more than 60%, the embedding productive rate is more than 65%, and its preparation process is as follows:
(1) soybean isolate protein powder of 5～8g is dissolved in 100mL, 50～65 ℃ of water, keep temperature-resistant and magnetic agitation 5～10min, ultrasonic 10～20min under 50～65 ℃ of conditions afterwards, with the mixed liquor that obtains centrifugal 20～30min under 10000～15000r, 4 ℃ condition, collect supernatant, and with the supernatant constant volume to 100mL, obtain soybean protein isolate solution;
(2) shitosan of 1～2g being dissolved in 100mL, volume fraction is in 1～3% the aqueous acetic acid, obtains the acetum of shitosan;
(3) 1～2g linoleic acid and 0.05～0.1g emulsifying agent are joined in the 100mL soybean protein isolate solution of step (1) preparation, mix with the acetum of the 100mL shitosan of step (2) preparation, with mixed liquor homogeneous 10～15min under the high-shear homogenizer of 6000～8000r, thereby form the emulsion of homogeneous, regulate the scope of emulsion pH to 4.5～4.8 with the hydrochloric acid of 1mol/L, magnetic agitation 10～15min again forms the aaerosol solution of microcapsules;
(4) regulate pH value to 7～7.3 of microcapsules aaerosol solution with the NaoH solution of 1mol/L, add 10～20mL, volume fraction then and be 50～60% glycerine water solution as curing agent, magnetic agitation is solidified 1～2h, leave standstill layering behind 1～3h, wet capsule is collected in suction filtration, distilled water washing back, obtains carrying linoleic soybean protein isolate/shitosan composite micro-capsule powder through vacuum freeze drying.
Wherein, described emulsifying agent can be glycerin monostearate, any one in the D-sorbite monoglyceride.Described suction filtration is that low speed filter paper filters in adopting, and collection obtains filter cake and washs through distilled water.Described vacuum freeze drying is filter cake that suction filtration is obtained vacuum less than 10Pa, cold sunken temperature-45～30 ℃ condition under drying totally.
The specific embodiment
Embodiment 1:
The soybean isolate protein powder of 5g is dissolved in 50 ℃ the hot water of 100mL, keep temperature-resistant and magnetic agitation 10min, move on in the ultrasonic device afterwards, 50 ℃ of ultrasonic 10min, the mixed liquor that obtains is centrifugal 30min under 10000r, 4 ℃ condition, collect supernatant, and supernatant is added the water constant volume to 100mL, obtain soybean protein isolate solution; It is in 1% the aqueous acetic acid that the shitosan of 1g is dissolved in the 100ml volume fraction, obtains the acetum of shitosan; 1.2g linoleic acid and 0.05g glycerin monostearate are joined in the above-mentioned 100mL soybean protein isolate solution for preparing, mix with the acetum of the above-mentioned 100mL shitosan for preparing, with mixed liquor homogeneous 10min under the high-shear homogenizer of 8000r, thereby form the emulsion of homogeneous, hydrochloric acid with 1mol/L is regulated emulsion pH to 4.5, magnetic agitation 15min again begins to take shape the aaerosol solution of microcapsules.Regulate the pH value to 7 of microcapsules aaerosol solution with the NaoH solution of 1mol/L, add the 15mL volume fraction and be 55% glycerine water solution as curing agent, magnetic agitation is solidified 2h, leave standstill layering behind the 2h, low speed filter paper suction filtration in the employing, suction filtration gained filter cake is collected wet capsule with distilled water washing back, in vacuum less than 10Pa, vacuum freeze drying totally under the condition of cold sunken temperature-45～30 ℃, obtain carrying linoleic soybean protein isolate/shitosan composite micro-capsule powder 4.795g, wherein contain soybean protein isolate 3.16g, chitosan-containing 0.8g contains linoleic acid 0.79g, contain emulsifying agent 0.045, powder order number is 80 orders, and its embedding efficiency is 61.2%, embedding productive rate 65.8%, and shelf life is long, is convenient to storage.
Embodiment 2:
The soybean isolate protein powder of 6g is dissolved in 55 ℃ the hot water of 100mL, keep temperature-resistant and magnetic agitation 12min, move on in the ultrasonic device afterwards, 55 ℃ of ultrasonic 10min, the mixed liquor that obtains is centrifugal 25min under 12000r, 4 ℃ condition, collect supernatant, and with the supernatant constant volume to 100mL; It is in 2% the acetum that the shitosan of 1.2g is dissolved in the 100ml volume fraction; 1.5g linoleic acid and 0.06g glycerin monostearate are joined in the above-mentioned 100mL soybean protein isolate solution for preparing, mix with the acetum of the above-mentioned 100mL shitosan for preparing, with mixed liquor homogeneous 15min under the high-shear homogenizer of 6000r, thereby form the emulsion of homogeneous, hydrochloric acid with 1mol/L is regulated emulsion pH to 4.5, magnetic agitation 15min again begins to take shape the aaerosol solution of microcapsules.Regulate the pH value to 7 of microcapsules aaerosol solution with the NaoH solution of 1mol/L, add the 15mL volume fraction and be 60% glycerine water solution as curing agent, magnetic agitation is solidified 2h, leave standstill layering behind the 3h, low speed filter paper suction filtration in the employing, filter cake is collected wet capsule with distilled water washing back, in vacuum less than 10Pa, vacuum freeze drying totally under the condition of cold sunken temperature-45～30 ℃, obtain carrying linoleic soybean protein isolate/shitosan composite micro-capsule powder 5.485g, wherein contain soybean protein isolate 3.42g, chitosan-containing 1g contains linoleic acid 0.99g, contain emulsifying agent 0.048, powder order number is 100 orders, and its embedding efficiency is 61.9%, embedding productive rate 66.3%, and shelf life is long, is convenient to storage.
Embodiment 3:
The soybean isolate protein powder of 8g is dissolved in 65 ℃ the hot water of 100mL, keep temperature-resistant and magnetic agitation 10min, move on in the ultrasonic device afterwards, 65 ℃ of ultrasonic 15min, the mixed liquor that obtains is centrifugal 20min under 15000r, 4 ℃ condition, collect supernatant, and with the supernatant constant volume to 100mL; It is in 3% the aqueous acetic acid that the shitosan of 2g is dissolved in the 100ml volume fraction; 2g linoleic acid and 0.1g D-sorbite monoglyceride are joined in the above-mentioned 100mL soybean protein isolate solution for preparing, mix with the acetum of the above-mentioned 100mL shitosan for preparing, with mixed liquor homogeneous 10min under the high-shear homogenizer of 8000r, thereby form the emulsion of homogeneous, hydrochloric acid with 1mol/L is regulated emulsion pH to 4.8, magnetic agitation 15min again begins to take shape the aaerosol solution of microcapsules.Regulate the pH value to 7.3 of microcapsules aaerosol solution with the NaoH solution of 1mol/L, add the 20mL volume fraction and be 60% glycerine water solution as curing agent, magnetic agitation is solidified 2h, leave standstill layering behind the 2h, low speed filter paper suction filtration in the employing, filter cake is collected wet capsule with distilled water washing back, in vacuum less than 10Pa, vacuum freeze drying totally under the condition of cold sunken temperature-45～30 ℃, obtain carrying linoleic soybean protein isolate/shitosan composite micro-capsule powder 7.538g, wherein contain soybean protein isolate 4.76g, chitosan-containing 1.43g contains linoleic acid 1.3g, contain emulsifying agent 0.048, powder order number is 100 orders, and its embedding efficiency is 62.4%, embedding productive rate 65.3%, and shelf life is long, is convenient to storage.
Claims (8)
1. preparation method of carrying linoleic soybean protein isolate/shitosan composite micro-capsule, its step is as follows:
(1) soybean isolate protein powder of 5～8g is dissolved in 100mL, 50～65 ℃ of water, keep temperature-resistant and magnetic agitation 5～10min, ultrasonic 10～20min under 50～65 ℃ of conditions afterwards, supernatant is collected in centrifugal back, and with the supernatant constant volume to 100mL, obtain soybean protein isolate solution;
(2) shitosan of 1～2g being dissolved in 100mL, volume fraction is in 1～3% the aqueous acetic acid, obtains the acetum of shitosan;
(3) 1～2g linoleic acid and 0.05～0.1g emulsifying agent are joined in the 100mL soybean protein isolate solution of step (1) preparation, mix with the acetum of the 100mL shitosan of step (2) preparation, with mixed liquor homogeneous 10～15min under 6000～8000r, thereby form the emulsion of homogeneous, regulate the scope of emulsion pH to 4.5～4.8, magnetic agitation 10～15min again forms the aaerosol solution of microcapsules;
(4) pH value to 7～7.3 of adjusting microcapsules aaerosol solution, add 10～20mL, volume fraction then and be 50～60% glycerine water solution as curing agent, magnetic agitation is solidified 1～2h, leave standstill layering behind 1～3h, wet capsule is collected in the filter cake distilled water washing back that suction filtration obtains, obtain carrying linoleic soybean protein isolate/shitosan composite micro-capsule powder through vacuum freeze drying.
2. the preparation method of a kind of year as claimed in claim 1 linoleic soybean protein isolate/shitosan composite micro-capsule is characterized in that: the described centrifugal centrifugal 20～30min that is the mixed liquor that will obtain under 10000～15000r, 4 ℃ condition of step (1).
3. the preparation method of a kind of year as claimed in claim 1 linoleic soybean protein isolate/shitosan composite micro-capsule is characterized in that: be the scope of regulating emulsion pH to 4.5～4.8 with the hydrochloric acid of 1mol/L in the step (3).
4. the preparation method of a kind of year as claimed in claim 1 linoleic soybean protein isolate/shitosan composite micro-capsule is characterized in that: step (4) is regulated pH value to 7～7.3 of microcapsules aaerosol solution with the NaoH solution of 1mol/L.
5. the preparation method of a kind of year as claimed in claim 1 linoleic soybean protein isolate/shitosan composite micro-capsule, it is characterized in that: the emulsifying agent in the step (3) is glycerin monostearate or D-sorbite monoglyceride.
6. the preparation method of a kind of year as claimed in claim 1 linoleic soybean protein isolate/shitosan composite micro-capsule is characterized in that: the described suction filtration of step (4) is that low speed filter paper filters in adopting, and collects to obtain filter cake and wash through distilled water.
7. the preparation method of a kind of year as claimed in claim 1 linoleic soybean protein isolate/shitosan composite micro-capsule is characterized in that: the described vacuum freeze drying of step (4) be filter cake that suction filtration is obtained vacuum less than 10Pa, cold sunken temperature-45～30 ℃ condition under drying totally.
8. one kind carries linoleic soybean protein isolate/shitosan composite micro-capsule, and it is characterized in that: any one preparation method prepares by claim 1～7.
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Cited By (11)
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| CN106689389A (en) * | 2016-12-05 | 2017-05-24 | 东北农业大学 | Preparation method of modified soy protein-polyphenol composite emulsion |
| CN106721849A (en) * | 2016-12-20 | 2017-05-31 | 青海览进生物科技开发有限公司 | A kind of preparation method of black fruit fructus lycii anthocyanidin Instant Drinks granule |
| CN107712587A (en) * | 2017-10-17 | 2018-02-23 | 广州润虹医药科技股份有限公司 | A kind of anti-oxidant anti-aging nutrient powder and preparation method thereof |
| CN108112954A (en) * | 2017-12-14 | 2018-06-05 | 成都新柯力化工科技有限公司 | A kind of microcapsule food sweetener and preparation method thereof |
| CN111955598A (en) * | 2020-08-28 | 2020-11-20 | 广州大学 | Method for preparing acid-soluble soybean protein by using homogenization method |
| CN112868978A (en) * | 2021-03-15 | 2021-06-01 | 吉林大学 | Curcumin nano-emulsion with fresh-keeping effect and preparation method thereof |
| CN114424820A (en) * | 2021-12-13 | 2022-05-03 | 东北农业大学 | Preparation method of low-fat perilla oil flavored salad sauce |
| CN115226765A (en) * | 2022-01-26 | 2022-10-25 | 四川农业大学 | Preparation method and application of enzyme-sensitive composite membrane |
| CN115252829A (en) * | 2022-08-07 | 2022-11-01 | 山西省人民医院 | Preparation method of ultrasonic contrast agent capable of targeting kidney cancer administration |
| CN116172086A (en) * | 2023-03-27 | 2023-05-30 | 河南工业大学 | Preparation method and application of novel quick-frozen special grease substitute grease |
| CN116172086B (en) * | 2023-03-27 | 2024-05-10 | 河南工业大学 | Preparation method and application of novel quick-frozen special grease substitute grease |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689389A (en) * | 2016-12-05 | 2017-05-24 | 东北农业大学 | Preparation method of modified soy protein-polyphenol composite emulsion |
| CN106721849A (en) * | 2016-12-20 | 2017-05-31 | 青海览进生物科技开发有限公司 | A kind of preparation method of black fruit fructus lycii anthocyanidin Instant Drinks granule |
| CN107712587A (en) * | 2017-10-17 | 2018-02-23 | 广州润虹医药科技股份有限公司 | A kind of anti-oxidant anti-aging nutrient powder and preparation method thereof |
| CN108112954A (en) * | 2017-12-14 | 2018-06-05 | 成都新柯力化工科技有限公司 | A kind of microcapsule food sweetener and preparation method thereof |
| CN111955598A (en) * | 2020-08-28 | 2020-11-20 | 广州大学 | Method for preparing acid-soluble soybean protein by using homogenization method |
| CN112868978A (en) * | 2021-03-15 | 2021-06-01 | 吉林大学 | Curcumin nano-emulsion with fresh-keeping effect and preparation method thereof |
| CN114424820A (en) * | 2021-12-13 | 2022-05-03 | 东北农业大学 | Preparation method of low-fat perilla oil flavored salad sauce |
| CN115226765A (en) * | 2022-01-26 | 2022-10-25 | 四川农业大学 | Preparation method and application of enzyme-sensitive composite membrane |
| CN115252829A (en) * | 2022-08-07 | 2022-11-01 | 山西省人民医院 | Preparation method of ultrasonic contrast agent capable of targeting kidney cancer administration |
| CN116172086A (en) * | 2023-03-27 | 2023-05-30 | 河南工业大学 | Preparation method and application of novel quick-frozen special grease substitute grease |
| CN116172086B (en) * | 2023-03-27 | 2024-05-10 | 河南工业大学 | Preparation method and application of novel quick-frozen special grease substitute grease |
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