CN103272006A - Preparation method for pulsatilla chinensis regel extracting solution and application of pulsatilla chinensis regel extracting solution - Google Patents
Preparation method for pulsatilla chinensis regel extracting solution and application of pulsatilla chinensis regel extracting solution Download PDFInfo
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- CN103272006A CN103272006A CN201310217246XA CN201310217246A CN103272006A CN 103272006 A CN103272006 A CN 103272006A CN 201310217246X A CN201310217246X A CN 201310217246XA CN 201310217246 A CN201310217246 A CN 201310217246A CN 103272006 A CN103272006 A CN 103272006A
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Abstract
The invention discloses a preparation method for a pulsatilla chinensis regel extracting solution. The preparation method is characterized by comprising the following steps of: smashing pulsatilla chinensis regel crude drugs into coarse powder, adding water according to a mass ratio of the coarse powder to the water of 1 to 5, and performing extraction by a soxhlet extractor; and evaporating extracted soup by a rotary evaporator, canning the soup into a brown bottle, and storing the bottle into a refrigerator for standby application. According to the preparation method for the pulsatilla chinensis regel extracting solution, the technology is simple, the steps are few, and industrial production is facilitated. By an illumination drifting method, the pulsatilla chinensis regel extracting solution has a very obvious effect on easing the pain of a mouse, so that the pulsatilla chinensis regel extracting solution has a pain easing pharmacological function; and a quick response is realized. Compared with the prior art, the method has the advantages that the technology is simple, the steps are few, and the industrial production is facilitated.
Description
Technical field
The present invention relates to the pharmaceutical technology field, be specifically related to a kind of preparation method and application thereof of Radix Pulsatillae extracting solution.
Background technology
The Radix Pulsatillae is the perennial Pulsatilla plant of the Ranunculaceae Radix Pulsatillae (chinese pulsatilla), perennial herb, another name has how, and grass, powder silkweed, hoary hair's grass, Lao Gucao, chrysanthemum chrysanthemum Seedling, old man spend, be preced with flower, the sub-flower of ram's horn etc. always, be distributed between hummock, barren hill and the field of economizing in Chinese Jilin, Liaoning, Hebei, Shandong, Henan, Shanxi, Shaanxi, Heilungkiang etc., beginning is stated from Shennong's Herbal, classify low-grades as, be conventional Chinese medicine, have the effect of heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, removing dampness and killing parasites.The feature of the Radix Pulsatillae is on the books in " Kaibao Bencao ": " the present tests this thick grass and gives birth to, and shape such as Radix Cynanchi Atrati are and soft and fine long slightly, and leaf is given birth to the stem head, as Folium Pruni, on tenderly white hair is arranged, nearly root person has white young pilose antler." tradition is mainly used in treating bacillary dysentery, amebic dysentery etc.Modern study is thought the Radix Pulsatillae except antibiotic and disease-resistant former effect, also have improve immunity of organisms, anticancer, antiinflammatory, antitoxin, antidiarrheal, calmness, ease pain, kill infusorian and coccidiosis, effects such as inhibition bowel movement, treating endotoxemia.
The chemical constituent of the Radix Pulsatillae is mainly compositions such as saponin and lactone.Comprising pulchinenoside, saponin, betulinic acid, betulic acid, arabopyranose glycosides, oxo betulic acid, daucosterol, protoanemonin, Radix Pulsatillae spirit, Radix Pulsatillae English etc.The multicomponent of Radix Pulsatillae medical material has determined many target spots of its therapeutical effect with multi-level.Radix Pulsatillae root contains anemoside (pulchinenoside) A, B, C, D, 3-O-α-L-pyrans rhamnose-(1 → 2)-α-L-arabopyranose-3 β, 23-dihydroxy-Δ 20(29)-lupene-28-acid (3-O-α-L-rhamnopyranosyl-(1 → 2) α-L-arabinopyranosyl-3 β, the 23-dihydroxylup Δ-20(29)-en-28-oicacid), anemoside (pulchinenoside) A3, B4, Saponin (saponin) 1,2, betulic acid-3-O-α-L arabopyranose glycoside (betulinicacid3-O-α-L-arabinopyranoside), betulic acid (betulinic acid), 3-oxo betulic acid (3-oxobetulinic acid), daucosterol (daucosterol), Anemonin (anemonin), protoanemonin (protoanemonin).
Radix Pulsatillae extracting solution comes from the treatise on Febrile Diseases of Zhang Zhongjing, and prescription is rigorous, the only Radix Pulsatillae, Rhizoma Coptidis, Cortex Phellodendri, Cortex Fraxini four Chinese medicine, and merit is arrogated to oneself positive clearly bright damp and hot, and the pyretic toxicity of blood system is separated in the stagnated fire of letting out the moon of fainting, and controls hygropyretic dysentery.Later age, normal alternative use was in multiple disease.As Sun Simiao in order to treat mass formed by blood stasis under the leucorrhea with bloody discharge; Wu brings up the logical diseases such as flavor damp and hotly due to the damp-warm diseases interior-deficiency sink in order to treat, diarrhea of heat type with tenesmus that add; "guide to clinical practice with medical record" carries: pathogenic warmth is puzzled through ten days, the heating diarrhea, and refreshing knowledge is unclear sometimes, and this evil the moon of fainting that lies prostrate probably causes the change convulsion, controls and should add the SHENGSHAO medicine by Radix Pulsatillae extracting solution; " side's of birdsing of the same feather flock together broad sense " meaning: it is hot and suffocating that Radix Pulsatillae extracting solution is controlled eyes again, red swollen paroxysmal pain, and the more than person of lacrimation induced by wind pathogen again for washing the steaming agent, is also imitated; Zhang Xichun controls the inferior in abdomen pain or the chronic dysentery intestinal with flexible Radix Pulsatillae extracting solution and rots.The modern digestive system disease such as dysentery, ulcerative colitis, chronic colitis, mouse typhus enteritis that are used for, also there is curative effect preferably Radix Pulsatillae extracting solution plus-minus side at aspects such as treatment diseases of urinary system, cardiovascular system diseases, disease of hematopoietic system, gynaecopathia, andropathy, ophthalmic diseasess simultaneously more.
Summary of the invention
The objective of the invention is to study in the curative effect of ease pain at the Radix Pulsatillae, a kind of Radix Pulsatillae extracting solution and application thereof are provided.
Technical scheme of the present invention is as follows:
A kind of preparation method of Radix Pulsatillae extracting solution is characterized in that, may further comprise the steps:
(1) gets Radix Pulsatillae crude drug root and be ground into coarse powder, add water by the mass ratio 1:4-6 of coarse powder and water, extract with apparatus,Soxhlet's;
(2) the decoction reuse Rotary Evaporators evaporation after extracting, it is standby that decoction is stored in refrigerator after with the brown bottle tinning.
Preferably, the particle diameter of the described coarse powder of step (1) is at 800 μ m.
Preferably, the mass ratio 1:5 of step (1) coarse powder and water.
Preferably, the decoction reuse Rotary Evaporators after the middle extraction of step (2) is evaporated to the 1/3-1/2 of stock solution.
The Radix Pulsatillae extracting solution of method for preparing has analgesic activity, can be that active component prepares analgesic with it, makes oral liquid.
The present invention will adopt the illumination tail-flick method to prove its analgesic effect in an embodiment.
Radix Pulsatillae extracting solution preparation method technology of the present invention is simple, and step is few, suitability for industrialized production.The present invention finds that by the illumination tail-flick method Radix Pulsatillae extracting solution can be fairly obvious to the analgesic activity that white mice produces, and Radix Pulsatillae extracting solution has the analgesic pharmacological action, and the time of effect is also very rapid.
The specific embodiment
Embodiment 1:
Get Radix Pulsatillae crude drug root 100g, be ground into coarse powder, add 500mL water, extract with apparatus,Soxhlet's.Decoction reuse Rotary Evaporators evaporation after the extraction, making concentration respectively is the high dose concentration group of 28.8g/kg and the low dosage concentration group that concentration is 10.8g/kg.It is standby that decoction is stored in refrigerator after with the brown bottle tinning.
Embodiment 2
Use embodiment 1 gained extracting solution to be used for experiment, in 16 ready white mice, get wherein 6 and get 6 again as low dose group and make not isolabeling as high dose group and labelling, all the other 4 is matched group.In the every morning 8:00-11:00 mice is irritated stomach, continue 5 days.Control group mice is irritated stomach with the distilled water of corresponding dosage.
The illumination tail-flick method:
, mice was carried out the illumination whipping survey the pain test in the 6th day after 5 days at the continuous irrigation stomach.Bulb power remains on 30W, the whipping behavior occurs as the pain indicator reaction with animal, and record begins to survey continuously 3 times to the incubation period that whipping occurs (s) from illumination, averages, and is called the whipping threshold.Prolongation of latency, namely the whipping threshold raises, and expression has analgesic activity.
Shown in table 1,2,3, the average whipping threshold of high dose group, low dose group and blank group is as shown in table 4 respectively for the incubation period of high dose group, low dose group and three tests of blank group mice and whipping threshold.
Table 1 high dose group
Table 2 low dose group
Table 3 blank group
The average whipping threshold of table 4
As can be seen, the average whipping threshold of low dose group white mice is apparently higher than the average whipping threshold of blank group mice.But the average whipping threshold of high dose group white mice is the average whipping threshold a little more than blank group mice.Can infer that thus the excessive concentration of the Radix Pulsatillae extracting solution of high dose causes Radix Pulsatillae extracting solution not obvious on the contrary to white mice analgesic effect.Under comparing, the optium concentration in the more approaching ideal of the concentration of low dosage, comparatively speaking, analgesic effect is more obvious.
With respect to other Chinese crude drug of analgesic activity is arranged, the difference of the analgesic effect performance of the Radix Pulsatillae is that the threshold of pain of high dose group mice is lower than the threshold of pain of low dose group mice on the contrary.And low dose group to show as onset slow, but also can reach more significant analgesic effect over time.Under same dosage condition, instead the mice ease pain of the Radix Pulsatillae of high dose group is lower than low dose group, and this possibility of result is relevant with the chemical constituent of the complexity of Radix Pulsatillae extracting solution, simultaneously, and also may be relevant with the pharmacological action of Radix Pulsatillae extracting solution.
The chemical constituent of Radix Pulsatillae extracting solution comprises alkaloids, Coumarins, Saponin class, limonin, sterols and lignanoids etc.Wherein alkaloids, Coumarins, Saponin class and limonoid are chemical constituent main in the Radix Pulsatillae extracting solution, also may bring into play the main matter basis of drug effect for decoction.
Wherein, the berberine hydrochloride of alkaloids has broad spectrum antibacterial function, can be used for treating multiple illness such as bacillary dysentery, enteritis, acute tonsillitis and acute bronchitis.Protozoacide, fungus and leishmania all there are inhibitory action, and have and discover that can treat acute diarrhea that cholera causes etc. with berberine discovers that berberine has preventive and therapeutic effect to the Animal diseases that the vibrio cholera enterotoxin causes.Other there are some researches show.Berberine has significant curative effect to the young rabbit diarrhoea that escherichia coli heat-stable toxin and heat-labile toxin cause.Under the effect of this chemical constituent, the many discomforts in the mice body are eliminated, and mice is had calmness, increase the effect of comfort, and simultaneously, also having increased from the side influences the mice analgesic.
Radix Pulsatillae extracting solution coumarin substances mainly comprises esculetin, aesculin etc.Esculetin has antioxidant activity, can remove peroxylradicals and hydroxyl radical free radical, suppresses the rabbit liver lipid peroxidation; Can suppress the degraded of collagen and protein sugar, treatment rheumatic arthritis and osteoarthritis.Aesculin can reduce vascular permeability and promote diuresis, has antiinflammatory, exudation, anthydropic effect, also can strengthen intravenous tension, antioxidant radical effect and neuroprotective etc. are arranged, but its antioxidant radical effect is weaker than esculetin.The existence of this material has directly influenced the analgesic activity of mice, and wherein the influence of antiinflammatory, exudation, anthydropic effect is particularly outstanding.
The contained Saponin of Radix Pulsatillae extracting solution has more than 5 kinds.That wherein content is maximum is Pulchinenoside A3B B4 (monosaccharide chain disaccharidase glycoside) and Saponin B4 (disaccharidase chain pentasaccharides glycoside).Oside compound can strengthen the phagocytic rate of macrophage.The enhancing human body immunity function has pharmacologically actives such as antioxidation, antiinflammatory, anti-trichomonal, antitumor.The functional promotion of immunity of organisms, and the effect of antiinflammatory have all increased the analgesic effect of mice.
Limonin in the Radix Pulsatillae extracting solution is mainly obakulactone.This compounds derives from Rhizoma Coptidis and Cortex Phellodendri, and obakulactone can delay the generation of graft-rejection, can also the inducing specific immunologic tolerance.
In addition, Radix Pulsatillae extracting solution also has some other compositions, but these chemical constituent correlational study reports are less.
Except the chemical composition analysis to Radix Pulsatillae extracting solution, also need the pharmacological action of Radix Pulsatillae extracting solution is considered.Up to the present; Pharmacological action study to Radix Pulsatillae extracting solution shows that Radix Pulsatillae extracting solution has: antibacterial action, antiinflammatory and repairing ulcer effect, immunoregulation effect, the motion of inhibition intestinal tube, diarrhea effect, strengthen immunocompetence effect in the body, to the therapeutical effect of digestive system disease, to liver protective effect, spermicide effect, to the therapeutical effect of diseases of urinary system, suppress macrophage secretion IL-6 effect etc.
In sum, Radix Pulsatillae extracting solution contained drug can promote non-specific immunity, antiinflammatory, antiviral, antidiarrheal, calmness, analgesia and inhibition bowel movement, can eliminate the pathogenic microorganism that causes damp-heat diarrhea.Also can suppress or local inflammatory disorders and discomfort when alleviating intestinal infection, can also promote the infection immunity function, influence course of infection from many aspects, thereby obtain good efficacy.
But the analgesic effect of high dose group mice is worse than the low dose group explanation, and the medicine of high dose makes the drug effect of mice descend.Can get rid of the possibility that overdose causes drug intoxication, because feeling sick does not appear in mice, vomiting, stupor, situations such as lethargy.Preliminary infer it is because overdose has caused the kickback of body, in the mode of urinating in a large number effective ingredient is got rid of external, thereby caused the unconspicuous result of analgesic effect.
Radix Pulsatillae extracting solution in use, there is a dose,optimum in and dose big making an appointment more with respect to a fixing body.In use, should be as far as possible near optimal dose in order to bring into play best analgesic effect.
Conclusion:
By the illumination tail-flick method, find that Radix Pulsatillae extracting solution can be fairly obvious to the analgesic activity that white mice produces, Radix Pulsatillae extracting solution has the analgesic pharmacological action.It should be noted that using Radix Pulsatillae extracting solution be to be attempted to high dose by low dosage., and studies show that according to other that time of effect is also very rapid because Radix Pulsatillae extracting solution analgesic activity is obvious.Therefore, the Radix Pulsatillae extracting solution of low dosage can reach the analgesic effect.On the contrary, the medicine of too high dose can cause analgesic effect to descend on the contrary.Tentatively think, be because the medicine of too high dose causes the kickback of mice body, and in the mode of urinating in a large number active substance excreted, thereby cause drug effect to descend.And the mice of low dose group there is no kickback, and medicine continues effect in vivo, so drug effect is obvious relatively.
The above is preferred embodiment of the present invention, but the present invention should not be confined to the disclosed content of this embodiment.So everyly do not break away from the equivalence of finishing under the spirit disclosed in this invention or revise, all fall into the scope of protection of the invention.
Claims (7)
1. the preparation method of a Radix Pulsatillae extracting solution is characterized in that, may further comprise the steps:
(1) gets Radix Pulsatillae crude drug root and be ground into coarse powder, add water by the mass ratio 1:4-6 of coarse powder and water, extract with apparatus,Soxhlet's;
(2) the decoction reuse Rotary Evaporators evaporation after extracting, it is standby that decoction is stored in refrigerator after with the brown bottle tinning.
2. the preparation method of the described Radix Pulsatillae extracting solution of claim 1 is characterized in that, the particle diameter of described coarse powder is at 800 μ m.
3. the preparation method of the described Radix Pulsatillae extracting solution of claim 1 is characterized in that, the mass ratio 1:5 of coarse powder and water.
4. the preparation method of the described Radix Pulsatillae extracting solution of claim 1 is characterized in that, the decoction reuse Rotary Evaporators after extracting in the step (2) is evaporated to the 1/3-1/2 of stock solution.
5. the Radix Pulsatillae extracting solution of any described method preparation of claim 1-4.
6. an analgesic is characterized in that, its active component is the described Radix Pulsatillae extracting solution of claim 5.
7. the described analgesic of claim 6 is characterized in that, its dosage form is oral liquid.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105535004A (en) * | 2016-02-02 | 2016-05-04 | 刘琦 | Application of anemoside B4 serving as EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs |
| CN111067911A (en) * | 2018-10-18 | 2020-04-28 | 刘琦 | Medical application of pulsatilla saponin B4 in resisting acute gouty arthritis |
| CN115916232A (en) * | 2020-06-05 | 2023-04-04 | 白周娟 | Composition for preventing or treating inflammatory diseases comprising hydrolyzed extract of Pulsatillae radix and anemone multocida as effective ingredient |
| RU2803967C1 (en) * | 2020-03-03 | 2023-09-25 | Ци Лю | Medical use of anemozide b4 in treatment of ulcers in oral cavity |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105535004A (en) * | 2016-02-02 | 2016-05-04 | 刘琦 | Application of anemoside B4 serving as EV71 virus inhibitor in preparing hand-foot-and-mouth disease resisting drugs |
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| CN111067911A (en) * | 2018-10-18 | 2020-04-28 | 刘琦 | Medical application of pulsatilla saponin B4 in resisting acute gouty arthritis |
| RU2803967C1 (en) * | 2020-03-03 | 2023-09-25 | Ци Лю | Medical use of anemozide b4 in treatment of ulcers in oral cavity |
| CN115916232A (en) * | 2020-06-05 | 2023-04-04 | 白周娟 | Composition for preventing or treating inflammatory diseases comprising hydrolyzed extract of Pulsatillae radix and anemone multocida as effective ingredient |
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