CN103267815B - Orthogonal axis counter current chromatograph - Google Patents
Orthogonal axis counter current chromatograph Download PDFInfo
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- CN103267815B CN103267815B CN201310162561.7A CN201310162561A CN103267815B CN 103267815 B CN103267815 B CN 103267815B CN 201310162561 A CN201310162561 A CN 201310162561A CN 103267815 B CN103267815 B CN 103267815B
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Abstract
The invention relates to a chemical machinery field, concretely an orthogonal axis counter current chromatograph, comprising a base, a drive device, a transmission device, a rotating device, a supporting device and a connector, wherein each device is arranged on the base, the drive device drives the rotating device to rotate by the transmission device, the rotating device comprises a main shaft, a rotating support, a gear system and three separating units, the three separating units are connected to the main shaft by the rotating support, and are axisymmetrically distributed relative to the main shaft, each separating unit comprises a separating column, the drive device makes the rotating device rotate around the main shaft by the transmission device, and the separating column carries out autorotation vertically to a revolution axis by the gear system while revoluting along the rotating device. The orthogonal axis counter current chromatograph of the invention can make sure the chromatograph to have good balance performance under a high-speed rotation state, thereby effectively raising separating efficiency.
Description
Technical field
The present invention relates to chemical machinery field, particularly relate to a kind of X-axis CPC instrument that can be applicable to the large molecule separation and purification of natural bioactive.
Background technology
In existing adverse current chromatogram isolation technics, X-axis CPC instrument is compared with planetary high speed chromatograph instrument, because the separating column axis of rotation of X-axis CPC instrument is mutually vertical with hollow shaft, this special mode of motion can form three-dimensional asymmetric centrifugal field, be highly suitable for the double-aqueous phase system that two property of liquid phase differences are little, the technical need of the large molecule separation and purification of natural bioactive such as protein, active peptides, nucleic acid, polysaccharide can be met, have a good application prospect.
X-axis CPC instrument of the prior art mainly comprises base, drive unit, gearing, whirligig, bracing or strutting arrangement and joint, whirligig is arranged on base by bracing or strutting arrangement, drive unit is rotated by gearing driven rotary device, joint is import and the outlet of sample, whirligig is generally provided with a separating column, also the separating column of two axis at straight line can be provided with, the X-axis CPC instrument of this structure is due to the setting position of separating column and integrally-built reason, there is balance difference, the problems such as separation efficiency is low.
Summary of the invention
(1) technical matters that will solve
The object of this invention is to provide a kind of X-axis CPC instrument, poor to overcome prior art balance, the problems such as separation efficiency is low.
(2) technical scheme
In order to solve the problems of the technologies described above, the invention provides a kind of X-axis CPC instrument, comprise base, drive unit, gearing, whirligig, bracing or strutting arrangement and joint, each device is arranged on base, described drive unit is rotated by gearing driven rotary device, described whirligig comprises main shaft, runing rest, gear train and three separative elements, described separative element to be connected on main shaft by runing rest and three separative elements distribute axisymmetricly relative to main shaft, described separative element comprises separating column, described drive unit makes whirligig rotate around the spindle by gearing, described separating column carries out the rotation with revolution axes normal by gear train while with whirligig revolution.
Preferably, described separative element comprises intermediate shaft, two connecting ducts and two separating columns, described intermediate shaft is between two separating columns and the axis of intermediate shaft and separating column and the axes normal of main shaft, described separating column comprises separation axis of a cylinder and separator tube, and described separator tube is wrapped on two separation axiss of a cylinder and its two ends respectively and is connected with two connecting ducts respectively.
Preferably, described gear train comprises bevel gear system, described bevel gear system turnkey draws together the bevel gear being located at bevel gear on base and being located on intermediate shaft, by the bevel gear of base to coordinate with the bevel gear of intermediate shaft main shaft can be made to rotate while intermediate shaft also carry out synchronous rotary.
Preferably, described gear train comprises spur gear system, described spur gear system comprises the spur gear be located on intermediate shaft, be located at the spur gear be separated on axis of a cylinder and the spur gear be located on runing rest, by the spur gear of the spur gear of intermediate shaft, the spur gear being separated axis of a cylinder and runing rest coordinate intermediate shaft can be made to rotate while two be separated axis of a cylinder and carry out reverse rotation.
Preferably, described runing rest comprises upper bracket, lower carriage, inner support and support arm, described inner support and support arm be positioned at each separative element two ends its respectively with intermediate shaft be separated axis of a cylinder and be rotationally connected, described upper bracket and lower carriage are positioned at the both sides up and down of separative element, and described upper bracket and lower carriage are fixedly connected with main shaft with inner support, support arm respectively.
Preferably, described bracing or strutting arrangement comprises bracing frame and three support columns, support frame as described above is fixedly connected on base by three support columns, and the upper end of described main shaft is connected with bracing frame by thrust ball bearing, and the lower end of described main shaft is connected with base by thrust ball bearing.
Preferably, described joint is two and is rotation seal joint, and a joint is positioned at the upside of bracing frame, and another joint is positioned at the downside of base.
Preferably, together, the two ends of the connecting duct linked together are connected with two joints the connecting duct serial or parallel connection of three separative elements respectively.
Preferably, described chromatograph also comprises shock attenuation device, and described shock attenuation device comprises rubber washer and butterfly spring, and described rubber washer is fixedly connected on base, described main shaft is connected with base by rubber washer, and described butterfly spring is located between bracing frame and support column.
Preferably, described chromatograph also comprises temperature regulating device, and described temperature regulating device comprises insulation can and air-conditioning, and it is outside and be fixedly connected on base that described insulation can covers on whirligig and bracing or strutting arrangement, described air-conditioning is connected with insulation can, for reducing the temperature in insulation can.
(3) beneficial effect
X-axis CPC instrument of the present invention is provided with the identical separative element of three structures, and three separative elements distribute axisymmetricly, the separating column of separative element carries out the rotation with revolution axes normal while whirligig revolution, this structure can ensure that chromatograph has good balance under the state of High Rotation Speed, effectively improve separation efficiency, and three separative elements make full use of revolution space, the present invention is specially adapted to the separation and purification of the large molecule of natural bioactive and bio-pharmaceutical, structure is simple, easy to operate, be easy to promote.
Accompanying drawing explanation
Fig. 1 is the stereographic map of the X-axis CPC instrument of the embodiment of the present invention;
Fig. 2 is the structural drawing of the X-axis CPC instrument of the embodiment of the present invention;
Fig. 3 is the structural drawing of the whirligig of the X-axis CPC instrument of the embodiment of the present invention.
In figure, 1: base; 2: arrangements for speed regulation; 3: drive unit; 4: insulation can; 5: whirligig; 6: air-conditioning; 7: synchronizing wheel; 8: Timing Belt; 9: synchronizing wheel; 10: rotation seal joint; 11: adapter bracket; 12: rubber washer; 13: chassis; 14: lower carriage; 15: bevel gear; 16: main shaft; 17: support column; 18: upper bracket; 19: thrust ball bearing; 20: bracing frame; 21: butterfly spring; 22: spring suppport; 23: adapter bracket; 24: rotation seal joint; 25: connecting duct; 26: support arm; 27: be separated axis of a cylinder; 28: separating column sleeve; 29: separator tube; 30: separating column disk; 31: inner support; 32: separating column support; 33: bevel gear; 34: spur gear; 35: spur gear; 36: round nut; 37: bearing; 38: intermediate shaft.
Embodiment
Below in conjunction with drawings and Examples, embodiments of the present invention are described in further detail.Following examples for illustration of the present invention, but can not be used for limiting the scope of the invention.
In describing the invention, it should be noted that, orientation or the position relationship of the instruction such as term " " center ", " on ", D score, " interior ", " outward " they be based on orientation shown in the drawings or position relationship; be only the present invention for convenience of description and simplified characterization; instead of instruction or imply the device of indication or element must have specific orientation, with specific azimuth configuration and operation, therefore can not be interpreted as limitation of the present invention.Unless otherwise clearly defined and limited, term " installation ", " being connected ", " connection " should be interpreted broadly, and such as, can be fixedly connected with, also can be removably connect, or connect integratedly; Can be mechanical connection, also can be electrical connection; Can be directly be connected, also indirectly can be connected by intermediary, can be the connection of two element internals.For the ordinary skill in the art, concrete condition above-mentioned term concrete meaning in the present invention can be understood.In addition, in describing the invention, except as otherwise noted, the implication of " multiple " is two or more.
As Figure 1-3, the X-axis CPC instrument of the present embodiment comprises base 1, drive unit 3, gearing, whirligig 5, bracing or strutting arrangement, shock attenuation device, arrangements for speed regulation 2, temperature regulating device and joint.Above-mentioned each device is all arranged on base 1, and drive unit 3 is rotated by gearing driven rotary device 5, and arrangements for speed regulation 2 are for the rotating speed of accessory drive 3, and joint is import and the outlet of sample.
Whirligig 5 comprises main shaft 16, runing rest, gear train and separative element, runing rest comprises upper bracket 18, lower carriage 14, inner support 31 and support arm 26, separative element is three and structure is identical, each separative element comprises intermediate shaft 38, two connecting ducts 25 and two separating columns, separating column comprises separation axis of a cylinder 27, separating column sleeve 28 and separator tube 29, gear train comprises bevel gear system and to unify spur gear system, bevel gear system turnkey draws together the bevel gear 15 be fixedly connected on base 1 and the bevel gear 33 being located at intermediate shaft 38 medial extremity, bevel gear can replace with the spiral bevel gear of the number of teeth and modulus correspondent equal, spur gear system comprises the spur gear being located at intermediate shaft 38 medial extremity, be located at three spur gears 35 on inner support 31 and be located at the spur gear 34 being separated axis of a cylinder medial extremity, described spur gear place is provided with bearing 37 and round nut 36, bearing 37 end is provided with screw thread, to ensure the axial restraint of bearing 37 and spur gear, wherein, spur gear can replace with the spiral gear of the number of teeth and modulus correspondent equal.Three separative elements to be connected on main shaft 16 by upper bracket 18 and lower carriage 14 and three separative elements distribute axisymmetricly relative to main shaft 16, the intermediate shaft 38 of separative element between two separating columns and the axis of intermediate shaft 38 and separating column all with the axes normal of main shaft 16, separating column sleeve 28 is enclosed within and is separated on axis of a cylinder 27, the two ends of separating column sleeve 28 are provided with separating column disk 30, separator tube 29 is wrapped on two separating column sleeves 28 respectively, the two ends of separator tube 29 (i.e. the import of separator tube 29 and outlet) are connected with two connecting ducts 25 respectively, two connecting ducts 25 are separately positioned on the two side ends that two are separated axis of a cylinder 27, inner support 31 and support arm 26 are three, lay respectively at the inside and outside two ends of each separative element, intermediate shaft 38 and the two ends being separated axis of a cylinder 27 are rotatably connected on inner support 31 and support arm 26 respectively, upper bracket 18 and lower carriage 14 are one, lay respectively at the both sides up and down of separative element and be fixedly connected with the two ends up and down of support arm 26 with inner support 31, upper bracket 18 is all fixedly connected with main shaft 16 with the inner side of lower carriage 14, by bevel gear 15 to coordinate with bevel gear 33 main shaft 16 can be made to rotate while intermediate shaft 38 also carry out synchronous rotary, by the spur gear 35 on an inner support of its side, the spur gear 34 be separated on axis of a cylinder 27 connects intermediate shaft 38 with one, described separation axis of a cylinder 27 can be made to carry out rotating Vortex with intermediate shaft 38, by the spur gear 35 on two inner supports of its opposite side, the spur gear 34 be separated on axis of a cylinder 27 connects intermediate shaft 38 with another, described separation axis of a cylinder 27 can be made to carry out reverse rotation with intermediate shaft 38, while intermediate shaft 38 just can be made thus to rotate, two separation axiss of a cylinder 27 carry out reverse rotation, the rotating speed of two separating columns is equal, turn to when can prevent separator tube 29 on the contrary from a separating column to another separating column and twist together, ensure that whole chromatographic normal operation.Drive unit 3 makes whirligig 5 rotate around main shaft 16 by gearing, and said structure can make separating column carry out the rotation with revolution axes normal by gear train while revolving round the sun with whirligig 5.
In above-mentioned whirligig 5, the center of gravity of each separative element was positioned at intermediate shaft 38 axis and in plane perpendicular to base 1, the center of gravity of single separating column is positioned on the axis of separation axis of a cylinder 27, the center of gravity of intermediate shaft 38 is positioned on its axis, the center of gravity of whole whirligig 5 is positioned on the axis of main shaft 16, ensure that reduction vibration under high-speed state, increase the stability of whole and part, thus there is good balance, and effectively improve separation efficiency, and the physical construction of three separative elements is identical in whirligig, be easy to realize modular design and manufacture.
Bracing or strutting arrangement comprises bracing frame 20 and three support columns 17, three support columns 17 in space relatively and main shaft 16 distribute axisymmetricly, bracing frame 20 is fixedly connected on base 1 by three support columns 17, the upper end of main shaft 16 is connected with bracing frame 20 by thrust ball bearing 19, the lower end of main shaft 16 is connected between bevel gear 15 and chassis 13 by thrust ball bearing and base 1, and chassis 13 is fixedly connected on base 1.
Joint is two, one is the entrance of sample, another is the outlet of sample, rotation seal joint 24 is fixedly connected on the upside of bracing frame 20 by adapter bracket 23, rotation seal joint 10 is fixedly connected on the downside of base 1 by adapter bracket 11, be convenient to separator tube 29 unwinding when passing in and out whirligig 5, when adopting dissimilar High Rotation Speed sealing adaptor, connecting duct 25 serial or parallel connection of three separative elements together, the two ends of the connecting duct linked together are connected with two joints the conversion of the series and parallel connections that can realize between separating column respectively, separation and purification simultaneously can be realized a kind of, two or three sample.
Shock attenuation device comprises rubber washer 12, butterfly spring 21 and spring suppport 22, rubber washer 12 is fixedly connected between base 1 and chassis 13, main shaft 16 is connected with base 1 by rubber washer 12, butterfly spring 21 is located between bracing frame 20 and support column 17 by spring suppport 22, and three butterfly springs 21 distribute axisymmetricly relative to main shaft 16 in space.
Temperature regulating device comprises insulation can 4 and air-conditioning 6, it is outside and be fixedly connected on base 1 that insulation can 4 covers on whirligig 5 and bracing or strutting arrangement, air-conditioning 6 is connected with insulation can 4, adopt air-conditioning 6 to cool the heat that when reducing whirligig 5 High Rotation Speed, gears meshing produces, in the interlayer in the middle of insulation can 4, add insulation material to keep the temperature in insulation can 4.
Drive unit 3 adopts the DC brushless motor with alternating current generator and direct current generator advantage, and this electric efficiency is high, stepless speed regulation, and speed adjustable range is wide, and overload capacity is strong.
The Timing Belt 8 that belt driver comprises the synchronizing wheel 7 be connected with drive unit 3, the synchronizing wheel 9 be connected with main shaft 16 and connects synchronizing wheel 7 and synchronizing wheel 9, this type of belt drive not only has V belt translation, chain drive and gear-driven advantage, and without relative sliding between Timing Belt and synchronizing wheel, stable drive ratio can be ensured, noise is less, can use and high speed situation.
Arrangements for speed regulation 2 are the special governing systems matched with brshless DC motor, also can be replaced by the gear ratio changed between synchronizing wheel 7 and synchronizing wheel 9.
Base 1 is a corrosion resistant plate, and lower end is provided with crossbeam, and four angles of steel plate are drilled with four holes, conveniently makes base 1 fix.
Embodiments of the invention provide in order to example with for the purpose of describing, and are not exhaustively or limit the invention to disclosed form.Many modifications and variations are apparent for the ordinary skill in the art.Selecting and describing embodiment is in order to principle of the present invention and practical application are better described, and enables those of ordinary skill in the art understand the present invention thus design the various embodiments with various amendment being suitable for special-purpose.
Claims (7)
1. an X-axis CPC instrument, comprise base, drive unit, gearing, whirligig, bracing or strutting arrangement and joint, each device is arranged on base, described drive unit is rotated by gearing driven rotary device, it is characterized in that, described whirligig comprises main shaft, runing rest, gear train and three separative elements, described separative element to be connected on main shaft by runing rest and three separative elements distribute axisymmetricly relative to main shaft, described separative element comprises separating column, described drive unit makes whirligig rotate around the spindle by gearing, described separating column carries out the rotation with revolution axes normal by gear train while with whirligig revolution,
Described separative element comprises intermediate shaft, two connecting ducts and two separating columns, described intermediate shaft is between two separating columns and the axis of intermediate shaft and separating column and the axes normal of main shaft, described separating column comprises separation axis of a cylinder and separator tube, and described separator tube is wrapped on two separation axiss of a cylinder and its two ends respectively and is connected with two connecting ducts respectively;
Described gear train comprises bevel gear system and to unify spur gear system, described bevel gear system turnkey draws together the bevel gear be located on base and the bevel gear be located on intermediate shaft, by the bevel gear of base to coordinate with the bevel gear of intermediate shaft main shaft can be made to rotate while intermediate shaft also carry out synchronous rotary, described spur gear system comprises the spur gear be located on intermediate shaft, be located at the spur gear be separated on axis of a cylinder and the spur gear be located on runing rest, by the spur gear of intermediate shaft, the spur gear being separated the spur gear of axis of a cylinder and runing rest coordinate intermediate shaft can be made to rotate while two be separated axis of a cylinder and carry out reverse rotation.
2. X-axis CPC instrument according to claim 1, it is characterized in that, described runing rest comprises upper bracket, lower carriage, inner support and support arm, described inner support and support arm be positioned at each separative element two ends its respectively with intermediate shaft be separated axis of a cylinder and be rotationally connected, described upper bracket and lower carriage are positioned at the both sides up and down of separative element, and described upper bracket and lower carriage are fixedly connected with main shaft with inner support, support arm respectively.
3. X-axis CPC instrument according to claim 2, it is characterized in that, described bracing or strutting arrangement comprises bracing frame and three support columns, support frame as described above is fixedly connected on base by three support columns, the upper end of described main shaft is connected with bracing frame by thrust ball bearing, and the lower end of described main shaft is connected with base by thrust ball bearing.
4. X-axis CPC instrument according to claim 3, is characterized in that, described joint is two and is rotation seal joint, and a joint is positioned at the upside of bracing frame, and another joint is positioned at the downside of base.
5. X-axis CPC instrument according to claim 4, is characterized in that, together, the two ends of the connecting duct linked together are connected with two joints the connecting duct serial or parallel connection of three separative elements respectively.
6. X-axis CPC instrument according to claim 5, it is characterized in that, described chromatograph also comprises shock attenuation device, described shock attenuation device comprises rubber washer and butterfly spring, described rubber washer is fixedly connected on base, described main shaft is connected with base by rubber washer, and described butterfly spring is located between bracing frame and support column.
7. according to the X-axis CPC instrument in claim 1-6 described in any one, it is characterized in that, described chromatograph also comprises temperature regulating device, described temperature regulating device comprises insulation can and air-conditioning, it is outside and be fixedly connected on base that described insulation can covers on whirligig and bracing or strutting arrangement, described air-conditioning is connected with insulation can, for reducing the temperature in insulation can.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310162561.7A CN103267815B (en) | 2013-05-06 | 2013-05-06 | Orthogonal axis counter current chromatograph |
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| CN201310162561.7A CN103267815B (en) | 2013-05-06 | 2013-05-06 | Orthogonal axis counter current chromatograph |
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| CN103267815A CN103267815A (en) | 2013-08-28 |
| CN103267815B true CN103267815B (en) | 2015-02-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109011695A (en) * | 2018-07-13 | 2018-12-18 | 汕头大学 | A kind of multi-functional tilting counter-current chromatograph of double rotational directions |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104941251B (en) * | 2015-05-19 | 2016-10-12 | 汕头大学 | A kind of X-axis CPC instrument |
| CN106153787B (en) * | 2016-09-06 | 2017-07-14 | 山东省分析测试中心 | A kind of multicolumn X-axis CPC instrument |
| CN106370761B (en) * | 2016-11-30 | 2017-10-24 | 汕头大学 | Dip-adjustable type counter-current chromatograph |
| CN107441769B (en) * | 2017-07-04 | 2019-12-06 | 汕头大学 | High-efficient reposition of redundant personnel chromatograph of many revolution axis separation columns |
| CN108037227B (en) * | 2017-12-01 | 2019-11-08 | 汕头大学 | High-speed counter-current chromatograph under a kind of electric field-enhanced effect |
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