CN108254477B - Multifunctional mixed structure type counter-current chromatograph - Google Patents
Multifunctional mixed structure type counter-current chromatograph Download PDFInfo
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Abstract
The invention relates to a multifunctional mixed structure type countercurrent chromatograph, which comprises a base, a supporting device, a driving device, a rotating device and a plurality of separation units, wherein the separation units comprise any combination of orthogonal separation units, parallel separation units and inclined separation units. The multifunctional mixed structure type countercurrent chromatograph is a new structure evolved by structurally combining the existing parallel axis, orthogonal axis and inclined axis high-speed countercurrent chromatographs. The planetary bevel gears with different included angles are adopted to realize revolution and rotation of the separation column with the orthogonal axis, the planetary bevel gears with different included angles are adopted to realize revolution and rotation of the separation column with the inclined axis, the planetary straight gears are adopted to realize revolution and rotation of the separation column with the parallel axis, and the motions of the separation columns with three different types are independent and do not influence each other. Has the advantages of strong adaptability, high separation efficiency, comprehensive functions and the like. Can be used for separating and purifying natural bioactive macromolecules, such as protein, active polypeptide, etc.
Description
Technical Field
The invention belongs to the field of chemical machinery, and particularly relates to a multifunctional mixed structure type counter-current chromatograph.
Background
Countercurrent chromatography is a solid carrier-free liquid-liquid chromatography technique applied in the separation field. Since it does not require a solid support, the separation of substances is achieved according to their different partition coefficients in the two immiscible liquids, since the problems of irreversible adsorption, inactivation and denaturation of the separated substances are avoided. And because the separated substance can be fully contacted with the liquid stationary phase, the sample loading quantity is greatly improved, and the method is an ideal preparation and separation means. The existing countercurrent chromatographs mainly include a parallel axis countercurrent chromatograph, an orthogonal axis countercurrent chromatograph and a tilt axis countercurrent chromatograph, and have respective advantages when aiming at different types of separated substances. However, none of the existing countercurrent chromatographs can simultaneously realize all functions of a set of equipment with three instruments, namely a parallel-axis countercurrent chromatograph, an orthogonal-axis countercurrent chromatograph and a tilted-axis countercurrent chromatograph.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a multifunctional mixed structure type counter-current chromatograph, which solves the problem that the same equipment in the prior art can only realize one mode of parallel-axis counter-current chromatography experiment or orthogonal-axis counter-current chromatography experiment or inclined-axis counter-current chromatography experiment, and has the advantages of strong adaptability, high separation efficiency, comprehensive functions and the like.
In order to solve the above problems, the present invention provides a multi-functional mixed structure type countercurrent chromatograph,
a multifunctional mixed structure type countercurrent chromatograph comprises a base, a supporting device, a driving device, a rotating device and a plurality of separation units, wherein the driving device and the supporting device are arranged on the base; the rotating device is fixedly arranged on the base through the supporting device; the rotating device comprises any combination of a main shaft, a rotating bracket, an orthogonal gear system, a parallel gear system and a tilting gear system; the rotating bracket is connected with the main shaft and is arranged on the base through a bearing; the orthogonal gear system, the parallel gear system and the inclined gear system are connected with the main shaft through the rotating bracket; the plurality of separation units comprise any combination of orthogonal separation units, parallel separation units and inclined separation units; the orthogonal separation unit comprises a plurality of orthogonal axis separation columns, the parallel separation unit comprises a plurality of parallel axis separation columns, and the inclined separation unit comprises a plurality of inclined axis separation columns; said pitch gear system being connected to said pitch shaft separation column; the parallel gear system is connected with the parallel shaft separation column; the orthogonal gear system is connected with the orthogonal shaft separation column; the driving device drives the main shaft to rotate, and the main shaft drives the orthogonal gear system, the parallel gear system and the inclined gear system, so that the rotation and revolution of the parallel shaft separation column, the inclined shaft separation column and the orthogonal shaft separation column are realized.
The multifunctional mixed structure type countercurrent chromatograph is a new structure evolved by structurally combining the existing parallel axis, orthogonal axis and inclined axis high-speed countercurrent chromatographs. The planetary bevel gears with different included angles are adopted to realize revolution and rotation of the separation column with the orthogonal axis, the planetary bevel gears with different included angles are adopted to realize revolution and rotation of the separation column with the inclined axis, the planetary straight gears are adopted to realize revolution and rotation of the separation column with the parallel axis, and the motions of the separation columns with three different types are independent and do not influence each other.
The separation column on the orthogonal axis separation unit can rotate around the axis of the separation column or revolve around the axis of a main shaft perpendicular to the axis of the separation column, and the orthogonal axis countercurrent chromatograph is equivalent to the orthogonal axis countercurrent chromatograph. The separation column on the parallel separation unit can rotate around the axis of the separation column and can also revolve around a spindle axis parallel to the axis of the separation column, and the parallel separation unit is equivalent to a parallel-axis countercurrent chromatograph. The separation column on the inclined shaft separation unit can rotate around the axis of the separation column and can also revolve around a spindle axis which has a certain included angle with the axis of the separation column, and the inclined shaft separation unit is equivalent to an inclined shaft counter-current chromatograph. Parallel axis countercurrent chromatography creates a two-dimensional centrifugal force field. The orthogonal axis type and the inclined axis type form a three-dimensional asymmetric centrifugal force field, and are more suitable for the stationary phase retention of a hydrophilic high-viscosity solvent system. Orthogonal-axis countercurrent chromatographic separation experiments can be carried out on the separation tube series orthogonal-axis separation column, parallel-axis countercurrent chromatographic separation experiments can be carried out on the separation tube series parallel-axis separation column, inclined-axis countercurrent chromatographic separation experiments can be carried out on the separation tube series inclined-axis separation column, and any two or three of the orthogonal-axis separation column, the parallel-axis separation column and the inclined-axis separation column can be simultaneously connected in series for carrying out mixed experiments.
The rotating support comprises an upper rotating support, a lower rotating support, an inner rotating support and an outer rotating support, the inclined shaft separation column and the parallel shaft separation column are mounted on the upper rotating support and the lower rotating support through bearing seats, the orthogonal shaft separation column is mounted on the inner rotating support and the outer rotating support through a second bearing and a second bearing seat, the inner rotating support and the outer rotating support are fixedly mounted on the upper rotating support and the lower rotating support through bolts, and the upper rotating support and the lower rotating support are fixedly mounted on the main shaft through second bolts. The whole support is connected with the main shaft and is arranged on the bottom plate through a bearing, so that the whole rotating support can rotate relative to the bottom plate to realize the revolution of the separation column. Because the separation column is installed on the rotating bracket through the bearing and the bearing seat, the separation column can rotate relative to the rotating bracket.
The parallel gear system comprises a fixed straight gear and a rotating straight gear; the fixed spur gear is fixedly arranged on the fixed disk, the fixed disk is arranged on the bottom plate through a bolt, and the main shaft is arranged on the bottom plate and the fixed bracket through a bearing and a bearing seat. The rotating spur gear is connected with the parallel shaft separation column, and the fixed spur gear is fixed with the bottom plate, so that the driving device drives the main shaft to rotate, the rotating spur gear revolves and is meshed with the fixed spur gear at the same time, and the rotation and revolution of the rotating spur gear and the parallel shaft separation column are realized.
The bevel gear system comprises a fixed arc bevel gear and a rotating arc bevel gear, the fixed arc bevel gear is fixedly arranged on an upper top disc, the upper top disc is arranged on a fixed support, and the fixed support is arranged on the bottom plate through a support column, so that the fixed arc bevel gear is fixed relative to the bottom plate. The rotating arc bevel gear is connected with the inclined shaft separation column, and the driving device drives the main shaft to rotate so that the rotating arc bevel gear revolves and is meshed with the fixed arc bevel gear at the same time, so that the rotation and revolution of the rotating arc bevel gear and the inclined shaft separation column are realized. The meshing included angle between the rotating arc bevel gear and the fixed arc bevel gear can be any angle from 0 degree to 90 degrees.
The orthogonal gear system comprises a fixed bevel gear, a rotating bevel gear and a straight gear, the fixed bevel gear is fixedly arranged on the lower chassis, and the lower chassis is arranged on the bottom plate through bolts. The rotary bevel gear is connected with a middle shaft, the middle shaft is arranged on an inner rotary support and an outer rotary support through bearings, the inner rotary support and the outer rotary support are arranged on an upper rotary support and a lower rotary support through bolts, the upper rotary support and the lower rotary support are fixedly arranged on a main shaft through bolts, and the main shaft is arranged on a bottom plate through a bearing and a bearing seat; the straight gear system comprises a driving straight gear, a driven straight gear and a plurality of intermediate straight gears, the driving straight gear is installed on an intermediate shaft, the driven straight gear is installed on a separation column of an orthogonal shaft, the intermediate straight gear is installed on an inner rotating support, the driving straight gear drives the intermediate straight gear, and the intermediate straight gear drives the driven straight gear to realize the equidirectional rotation of the driven straight gear and the driving straight gear. The driving straight gear drives two middle straight gears, and the middle straight gears drive the driven straight gears to realize the reverse rotation of the driven straight gears and the driving straight gears. The driving direct drive driven straight gear realizes the reverse rotation of the driven straight gear and the driving straight gear; when the driving device drives the main shaft to rotate, the rotating bevel gear revolves and is simultaneously meshed with the fixed bevel gear to realize rotation of the rotating bevel gear and the driving straight gear, the driving straight gear drives the middle straight gear, and the middle straight gear drives the driven straight gear or the driving straight gear directly drives the driven straight gear to realize rotation and revolution of the driven straight gear and the orthogonal shaft separation column. The included angle between the rotary bevel gear and the fixed bevel gear is fixed to be 90 degrees.
Separation tubes are wound on the parallel axis separation column, the inclined axis separation column and the orthogonal axis separation column; the separation materials can be subjected to parallel-axis countercurrent chromatography separation experiment or orthogonal-axis countercurrent chromatography separation experiment or inclined-axis countercurrent chromatography separation experiment respectively through the separation tubes in parallel; experiments can also be carried out by connecting the separation tubes in series and simultaneously carrying out any two or three of parallel-axis countercurrent chromatography separation experiments, orthogonal-axis countercurrent chromatography separation experiments and inclined-axis countercurrent chromatography separation experiments.
When the separation pipes are connected in series, the two adjacent separation columns are connected end to end or head to end when the rotation directions are opposite, and the two adjacent separation columns are connected end to end when the rotation directions are the same, so that the separation pipes on the separation columns are used for active descrambling.
Compared with the prior art, the invention designs the multifunctional mixed structure type countercurrent chromatograph by taking advantage of the advantages of the traditional parallel axis, orthogonal axis and inclined axis countercurrent chromatographs, and the instrument can simultaneously have a plurality of parallel axis separation columns, a plurality of orthogonal axis separation columns and a plurality of inclined axis separation columns. The device can be used for carrying out parallel-axis countercurrent chromatography separation experiments or orthogonal-axis countercurrent chromatography separation experiments or inclined-axis countercurrent chromatography separation experiments on the separated substances in parallel through the separation tubes. The apparatus can also be used for simultaneously carrying out any two or three of parallel-axis countercurrent chromatography separation experiments, orthogonal-axis countercurrent chromatography separation experiments and inclined-axis countercurrent chromatography separation experiments in series through the separation tubes. The multifunctional chromatographic instrument has the advantages of realizing multiple purposes of one instrument, greatly improving the separation efficiency of the chromatographic instrument, having strong adaptability, high separation efficiency, comprehensive functions and the like; can be used for separating and purifying natural bioactive macromolecules, such as protein, active polypeptide, etc.
Drawings
FIG. 1 is an overall view of an orthogonal axis parallel combination of a multi-functional mixed structure type countercurrent chromatograph according to the present invention;
FIG. 2 is a view showing a parallel-axis orthogonal-axis combination structure of the multi-functional mixed structure type countercurrent chromatograph of the present invention;
FIG. 3 is a partial view of a parallel-axis and orthogonal-axis combination of a multi-functional mixed-structure type countercurrent chromatograph according to the present invention;
FIG. 4 is a view of the structure of the inclined-axis and orthogonal-axis combination of the multi-functional mixed structure type countercurrent chromatograph of the present invention;
FIG. 5 is a combined structure diagram of the inclined axis and the parallel axis of the multi-functional mixed structure type countercurrent chromatograph of the present invention;
FIG. 6 is a view showing the structure of a parallel axis assembly of orthogonal axis inclined axes of a multi-functional mixed structure type countercurrent chromatograph according to the present invention;
FIG. 7 is a transmission diagram of the combination of orthogonal axis, inclined axis and parallel axis of the multi-functional mixed structure type countercurrent chromatograph of the present invention;
in the figure: 1. the device comprises a base, 2, a motor, 3, support columns, 4, a fixed support, 5, a synchronous pulley 6, a main shaft, 7, a lower chassis, 8, an outer rotary support, 9, an upper rotary support, 10, an inner rotary support, 11, a fixed spur gear, 12, an upper top disk, 13, a rotary spur gear, 14, an orthogonal-axis separation column, 14-1, an orthogonal-axis separation column, 15, a lower rotary support, 16, a parallel-axis separation column, 17, a separation pipe, 18, a driven spur gear, 19, an intermediate spur gear, 20, a driving spur gear, 21, an intermediate shaft, 22, a rotary bevel gear, 23, a fixed bevel gear, 24, a fixed bevel gear, 25, a rotary bevel gear, 26 and an inclined-axis separation column.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.
Example 1
Parallel-axis orthogonal-axis combination mode
The structure of this embodiment is as shown in fig. 1, and includes base 1, motor 2, support column 3, fixed bolster 4, and whole rotation module passes through support column 3 fixed mounting on base 1.
The rotating support comprises an upper rotating support 9, a lower rotating support 15, an inner rotating support 10 and an outer rotating support 8, the inner rotating support 10 and the outer rotating support 8 are fixedly arranged on the upper rotating support 9 and the lower rotating support 15, and the upper rotating support 9 and the lower rotating support 15 are fixedly arranged on the main shaft 6.
The planetary straight gear system comprises a fixed straight gear 11 and a rotating straight gear 13, wherein the fixed straight gear 11 is fixedly arranged on the upper top disc 12, the rotating straight gear 13 is connected with the parallel shaft separation column 16, and when the driving motor 2 drives the main shaft 6 to rotate, the rotating straight gear 13 is meshed with the fixed straight gear 11 to realize rotation and revolution of the parallel shaft separation column 16.
The planetary bevel gear system comprises a fixed bevel gear 23 and a rotating bevel gear 22, wherein the fixed bevel gear 23 is fixedly installed on the lower chassis 7, the rotating bevel gear 22 is connected with the intermediate shaft 21, and when the driving motor 2 drives the main shaft 6 to rotate, the rotating bevel gear 22 is meshed with the fixed bevel gear 23 to realize rotation and revolution of the intermediate shaft 21.
The straight gear system comprises a driving straight gear 20, a driven straight gear 18 and a plurality of intermediate straight gears 19, wherein the driving straight gear 20 is arranged on an intermediate shaft 21, the driven straight gear 18 is arranged on the orthogonal shaft separation columns 14, and the intermediate straight gears 19 are arranged on the inner rotating bracket 10 through bearings to change the autorotation directions of the orthogonal shaft separation columns 14.
The parallel shaft separation unit comprises two parallel shaft separation columns 16 which are arranged on the upper and lower rotating supports 9 and 15 through bearings, when the parallel shaft separation unit works, the parallel shaft separation columns 16 can revolve around the main shaft 6 and can also rotate around the self shaft, and the rotation axis is parallel to the axis of the main shaft.
The orthogonal axis separation unit includes four orthogonal axis separation columns 14 mounted on the inner and outer rotation brackets 10 and 8 through bearings, the orthogonal axis separation columns 14 can revolve around the main axis 6 and rotate around their own axis, and the rotation axis is perpendicular to the main axis.
The separation tube 17 is introduced through the main shaft 6 to bypass the orthogonal-axis separation columns 14, and since the direction of rotation of the adjacent two orthogonal-axis separation columns is opposite, the parallel-axis separation column 16 sandwiched between the adjacent two orthogonal-axis separation columns 14 and 14-1 must be in the same direction as the direction of rotation of the adjacent one of the orthogonal-axis separation columns 14 and in the opposite direction to the direction of rotation of the other orthogonal-axis separation column 14-1, so that the separation tube 17 must be in end-to-end or end-to-end connection when the directions of rotation of the two adjacent separation columns are opposite, and in end-to-end connection when the directions of rotation of the two adjacent separation columns are the same, thus actively descrambling by using the separation tubes on the separation columns.
During operation, the driving motor 2 drives the main shaft 6 to rotate to drive the whole rotating module to rotate, the fixed straight gear 11 is fixedly arranged on the upper top disc 12, the fixed bevel gear 23 is fixedly arranged on the lower bottom disc 7, the rotating straight gear 13 is connected with the parallel shaft separation column 16, and when the driving motor 2 drives the main shaft 6 to rotate, the rotating straight gear 13 is meshed with the fixed straight gear 11 to realize rotation and revolution of the parallel shaft separation column 16. The rotating bevel gear 22 is connected with the intermediate shaft 21, when the driving motor 2 drives the main shaft 6 to rotate, the rotating bevel gear 22 is meshed with the fixed bevel gear 23 to realize rotation and revolution of the intermediate shaft 21, and the driving spur gear 20 on the intermediate shaft 21 drives the driven spur gear 20 on the orthogonal-axis separation column 14 through the intermediate spur gear 19 to realize reverse rotation of the adjacent two orthogonal-axis separation columns 14 and 14-1 to realize unwinding of the separation tubes between the adjacent two separation columns.
Example 2
Orthogonal axis combination mode of inclined axis
The oblique-axis orthogonal-axis combination is similar to the parallel-axis orthogonal-axis combination as shown in fig. 4. The embodiment of the orthogonal-axis separation unit has been described in detail in example 1, focusing on the embodiment of the inclined-axis separation unit. The fixed arc bevel gear 24 is fixedly arranged on the upper rotating bracket, the rotating arc bevel gear 25 is fixedly connected with the inclined shaft separation column 26, and when the rotating arc bevel gear 25 and the fixed arc bevel gear 24 are meshed to realize rotation and revolution of the inclined shaft separation column while the orthogonal shaft separation column rotates in work.
Example 3
Combination mode of inclined shaft and parallel shaft
The tilt axis-parallel axis combination is shown in fig. 5, and is similar to the above two embodiments, and the embodiment of the tilt axis-separating unit has been described in example 2, and the parallel axis-separating unit is similar to the parallel separating unit in example 1. The difference lies in that the top of the chromatograph is changed to the bottom of the chromatograph at the installation position of the fixed spur gear and the rotary spur gear.
Example 4
Orthogonal axis inclined axis and parallel axis combination mode
The orthogonal axis inclined shaft parallel shaft combination mode is as shown in fig. 6 and 7, the orthogonal axis inclined shaft parallel shaft combination mode is similar to the three modes, the orthogonal axis separation unit is similar to the embodiment of the embodiment 1, the difference is that the driving spur gear is driven while the rotary bevel gear rotates, and the driving spur gear does not directly drive the driven spur gear through the middle spur gear to realize the rotation and the revolution of the orthogonal axis separation column. An embodiment of the tilt axis separation unit has been described in example 2. The parallel axis separation unit is similar to that in example 1. The difference lies in that the top of the chromatograph is changed to the bottom of the chromatograph at the installation position of the fixed spur gear and the rotary spur gear.
Claims (4)
1. A multifunctional mixed structure type countercurrent chromatograph is characterized by comprising a base, a supporting device, a driving device, a rotating device and a plurality of separation units, wherein the driving device and the supporting device are arranged on the base; the rotating device is fixedly arranged on the base through the supporting device; the rotating device comprises any combination of a main shaft, a rotating bracket, an orthogonal gear system, a parallel gear system and a tilting gear system; the rotating bracket is connected with the main shaft and is arranged on the base through a bearing; the orthogonal gear system, the parallel gear system and the inclined gear system are connected with the main shaft through the rotating bracket; the plurality of separation units comprise any combination of orthogonal separation units, parallel separation units and inclined separation units; the orthogonal separation unit comprises a plurality of orthogonal axis separation columns, the parallel separation unit comprises a plurality of parallel axis separation columns, and the inclined separation unit comprises a plurality of inclined axis separation columns; said pitch gear system being connected to said pitch shaft separation column; the parallel gear system is connected with the parallel shaft separation column; the orthogonal gear system is connected with the orthogonal shaft separation column; the driving device drives the main shaft to rotate, and the main shaft drives the orthogonal gear system, the parallel gear system and the inclined gear system, so that the rotation and revolution of the parallel shaft separation column, the inclined shaft separation column and the orthogonal shaft separation column are realized; the rotating bracket comprises an upper rotating bracket, a lower rotating bracket, an inner rotating bracket and an outer rotating bracket, the inclined shaft separation column and the parallel shaft separation column are arranged on the upper rotating bracket and the lower rotating bracket through bearing seats, the orthogonal shaft separation column is arranged on the inner rotating bracket and the outer rotating bracket through a second bearing and a second bearing seat, the inner rotating bracket and the outer rotating bracket are fixedly arranged on the upper rotating bracket and the lower rotating bracket through bolts, and the upper rotating bracket and the lower rotating bracket are fixedly arranged on the main shaft through bolts; the parallel gear system comprises a fixed straight gear and a rotating straight gear; the fixed spur gear is fixedly arranged on the base; the driving device drives the main shaft to rotate, so that the rotating spur gear revolves and is meshed with the fixed spur gear at the same time, and the rotation and revolution of the rotating spur gear and the parallel shaft separation column are realized; the inclined gear system comprises a fixed arc bevel gear and a rotating arc bevel gear, the fixed arc bevel gear is fixedly installed on the base, the rotating arc bevel gear is connected with the inclined shaft separation column, and the driving device drives the main shaft to rotate so that the rotating arc bevel gear revolves and is meshed with the fixed arc bevel gear at the same time, so that the rotation and revolution of the rotating arc bevel gear and the inclined shaft separation column are realized; the orthogonal gear system comprises a fixed bevel gear, a rotating bevel gear and a straight gear, and the fixed bevel gear is fixedly arranged on the bottom plate; the rotary bevel gear is connected with a middle shaft, and the middle shaft is arranged on the inner rotary bracket and the outer rotary bracket; the straight gear system comprises a driving straight gear, a driven straight gear and a plurality of intermediate straight gears, the driving straight gear is arranged on an intermediate shaft, the driven straight gear is arranged on a separation column with an orthogonal shaft, and the intermediate straight gears are arranged on an inner rotating bracket to change the rotation direction of the separation column; when the driving device drives the main shaft to rotate, the rotating bevel gear revolves and is meshed with the fixed bevel gear at the same time, and the rotation of the rotating bevel gear and the driving straight gear is realized.
2. The multifunctional mixed structure type countercurrent chromatograph of claim 1, wherein the angle between the meshing angle of the rotating bevel arc gear and the fixed bevel arc gear can be any angle from 0 ° to 90 °.
3. The multifunctional mixed structure type countercurrent chromatograph of claim 1, wherein said parallel axis separation column, inclined axis separation column, and orthogonal axis separation column are each surrounded by a separation tube; the separation materials can be subjected to parallel-axis countercurrent chromatography separation experiment or orthogonal-axis countercurrent chromatography separation experiment or inclined-axis countercurrent chromatography separation experiment respectively through the separation tubes in parallel; experiments can also be carried out by connecting the separation tubes in series and simultaneously carrying out any two or three of parallel-axis countercurrent chromatography separation experiments, orthogonal-axis countercurrent chromatography separation experiments and inclined-axis countercurrent chromatography separation experiments.
4. The multifunctional mixed structure type countercurrent chromatograph of claim 3, wherein said separation tubes are connected in series, end-to-end or end-to-end when the rotation directions of two adjacent separation columns are opposite, and end-to-end when the rotation directions of two adjacent separation columns are the same.
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| CN109011695A (en) * | 2018-07-13 | 2018-12-18 | 汕头大学 | A kind of multi-functional tilting counter-current chromatograph of double rotational directions |
| CN110346491B (en) * | 2019-07-23 | 2021-07-13 | 汕头大学 | Multifunctional dip angle adjustable counter-current chromatograph |
| CN110327660B (en) * | 2019-07-23 | 2021-07-13 | 汕头大学 | Transmission structure of counter-current chromatograph with adjustable inclination angle |
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| US4058460A (en) * | 1977-03-17 | 1977-11-15 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Horizontal flow-through coil planet centrifuge without rotating seals |
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| CN106370761B (en) * | 2016-11-30 | 2017-10-24 | 汕头大学 | Dip-adjustable type counter-current chromatograph |
| CN107441769B (en) * | 2017-07-04 | 2019-12-06 | 汕头大学 | High-efficient reposition of redundant personnel chromatograph of many revolution axis separation columns |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4058460A (en) * | 1977-03-17 | 1977-11-15 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Horizontal flow-through coil planet centrifuge without rotating seals |
Non-Patent Citations (1)
| Title |
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| 正交轴水平流通式逆流色谱仪的研究;张天佑 等;《仪器仪表学报》;19900228;第11卷(第1期);第16-23页 * |
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