CN103265145B - Method for purifying river water with low load and low temperature by using natural biofilm reactor - Google Patents

Method for purifying river water with low load and low temperature by using natural biofilm reactor Download PDF

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CN103265145B
CN103265145B CN201310183787.5A CN201310183787A CN103265145B CN 103265145 B CN103265145 B CN 103265145B CN 201310183787 A CN201310183787 A CN 201310183787A CN 103265145 B CN103265145 B CN 103265145B
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CN103265145A (en
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吴永红
陆海鹰
王金花
刘志彬
陈建贞
李运东
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Institute of Soil Science of CAS
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Abstract

The invention belongs to the field of ecological and environmental protection, relates to the field of purification of low-load river water in a field area, and particularly relates to a method for purifying river water with low load and low temperature by using a natural biofilm reactor. The method comprises the following steps of: (1) gathering and cultivating of a natural biofilm, namely carrying out gathering and cultivating of microorganisms such as algae and bacteria by directly utilizing riverway water as a 'seed source'; (2) low-temperature natural biofilm domestication, namely reducing the cultivating temperature at the subdivision stage of the pre-cultivated natural biofilm, wherein the temperature reducing gradient is 1 DEG C reduced every two days until the cultivation temperature is reduced to 0-4 DEG C; (3) low-temperature inoculation of the natural biofilm to a reactor; (4) debugging and operating of the natural biofilm reactor, namely adjusting hydraulic load and standing time of the reactor by adjusting the weight of the floating body and the reactor; and (5) water purification by the natural biofilm reactor. The method is simple and feasible, good in purification effect of riverway water in the field area, and convenient to popularize; and meanwhile, the problem of low removal efficiency of pollutants in winter is solved.

Description

Utilize natural biology membrane reactor to purify the method for underload low temperature river
Technical field
The invention belongs to ecology and field of environment protection, relate to underload river water cleaning field, section, farmland, relate in particular to a kind of method of utilizing natural biology membrane reactor to purify underload low temperature river.
Background technology
At present, body eutrophication problem is just having a strong impact on the mankind's living environment.Over nearly 10 years, China's rivers, storehouse, lake eutrophication are very serious and be degradating trend.In more than 700 river of China (overall length 100,000 kms), 70.6% river is polluted.It is dangerous that China rural area has people more than 300,000,000 to drink water, and wherein approximately more than 6,300 ten thousand people drink water of high fluorine content, and 2,000,000 people drink high arsenic water, and more than 3,800 ten thousand people drink brackish water, and 1.9 hundred million people's tap water harmful substance contents exceed standard, and Schistosomiasis Endemic Areas approximately more than 1,100 ten thousand people drink water dangerous; Quite a few urban water resource is seriously polluted, threatens quality of drinking water.
Farmland pollution of area source is one of China's principal mode of entering dystrophication load, and the discharge of farmland pollution of area source has brought the pollutent of a lot of underloads to river course nearby.Due to this pollutant underload, brought a lot of new challenges to conventional treatment process, the cost performance of processing as traditional activated sludge process is not high.
At present, for the treatment process of this class underload river, be divided into two classes, a class is original position purification of water quality method, and another kind of is the bypass method of purification (claiming again dystopy facture).The bypass method of purification is to utilize the vacant lot on both sides, river course, builds river water cleaning system, as wet land treating system, biological pond, soil filtration etc.For example: for what cut down Dian Chi, enter lake pollution load, the thinking purifying based on bypass has designed and built the biological stabilization pond system that comprises Aquatic-plant Pond and culture pond, has investigated the removal effect to nitrogen, phosphorus.Result shows, this system is to TN, TP, NO 3 --N and NH 3the average removal rate of-N is respectively 25.3%, 50.6%, 38.4% and 35.6%, also has certain ecoscape benefit when improving water quality, and the purification that can be other water body provides demonstration.The feature of the bypass method of purification is exactly to build economically viable treatment system in conjunction with river water quality feature, but this class bypass method of purification needs power water lift conventionally, has increased processing costs, also needs to increase manpower and manages and protects.
Therefore, there is research that this bypass purifying method is carried out to upgrading, as construction that bypath system is combined with traditional sewage purification system (as denitrification dephosphorization system).The problems such as patent of invention (application number 201110159014.4) is high for the water inlet sediment concentration often running in current denitrification dephosphorization technique, VSS/MLSS is on the low side, carbon source is not enough propose a kind of bypass mud decrement, silt separation and denitrogenation dephosphorizing Sewage treatment systems and method, main body Sewage treatment systems and the bypath system two portions with denitrogenation dephosphorizing function, consist of.Main body Sewage treatment systems comprises settling pit and has the Sewage treatment systems of denitrogenation dephosphorizing function.Bypath system is comprised of hair separator, mud decrement pond, mud silt separator and silt dewatering system and chemical phosphorus removal system.This way that combines with bypass clean-up system and build, can prevent that silt from accumulating in Sewage treatment systems, promote mud VSS/MISS ratio in biochemical reaction tank, in embedded bypath system, be aided with chemical dephosphorization, can realize the low cost chemistry of sewage phosphorus and remove and reclaim.
Yet for the river sewage of low pollution load, cleaning project is built in the large-area wasteland of this method needs that relate to bypass clean-up system, in the area of land resources deficiency or the area of land resources costliness should not adopt.
Given this, in actual riverway water quality purification case, the application of original position purification of water quality method is more extensive, also has reasonable prospect.From ecological angle, original position is improved water quality, improves self-purification capacity and the restorer of river course aquatic ecosystem, is also the only way of river channel ecology system sustainable and healthy development.
Original position purification of water quality method mainly utilizes biomembrance process principle to purify river water quality.Biomembrance process is a kind of important method for biologically treating wastewater, and it is to rely on the microflora be attached on carrier to absorb and N, P, COD and other pollution substance of degrading waste water reach decontamination effect improving.Under anaerobic, anaerobion can be processed the waste water of high density within a short period of time and larger molecular organics matter is degraded to low molecular compound, and then is converted into methane, carbonic acid gas; Under aeration condition, aerobic microbiological can reduce in water COD and absorb N, P and reaches the effect of purifying waste water.
Due to bio-reactor fixed biofilm well, regulate microbial film to form structure; Can control biomembranous biomass and activity by regulating the setting of reactor, therefore, bio-reactor in position in purification of water quality application more extensive, also in river original position field of purification, there is good prospect.By concluding, this class utilizes the typical case that reactor is purified waste water to comprise as follows:
Sequencing batch submerged membrane bio-reactor (patent of invention number: 03133571.3), realize aerobic, anoxic, three kinds of environment of anaerobism in same reaction tank, nitrogen and phosphorus pollutants is fully removed.
Batched membrane-bioreactor effluent treatment process and device (patent of invention number: 03100398.2) put into membrane module in biological reaction tank, and its outlet is connected respectively with rising pipe with inlet pipe by two groups of by-pass valve controls, whole system alternately experiences anaerobism, aerobic, anoxic condition in time, can carry out synchronously effectively removing to nutritive elements such as carbon containing organic pollutant and nitrogen, phosphorus.
Multi-stage biological reactor (patent of invention number: 201080057245.8) the multi-stage biological reactor in settling tank, each cell comprises framework and a plurality of net substrate that is mechanically secured to framework of hollow, substrate is arranged to growth microorganism on it.
The flow lifting type microorganism reactor treatment process (patent of invention number: 201210176953.4) first Mn-bearing waste water enters primary clarifier through submersible pump of Mn-bearing waste water, after elementary precipitation process, then via in-line pump, enter continuously the aerobe reaction tank of two series connection, water inlet is controlled by intake pump, the water of discharging from the reaction tank of series connection then enters three layer filtration pond, and the water of discharging from three layer filtration pond is the clear water after processing.
(the patent of invention number: while 201210014485.0) having solved existing Membrane Bioreactor for Wastewater Treatment micro-polluted surface water of the regulate and control method of microorganism active in membrane bioreactor, because former water organic concentration is lower or the reason such as low temperature causes biological activity and biomass in membrane bioreactor to reduce, the problem of water treatment deleterious.
Non-woven fabrics flco bioreactor of dynamic membrane (patent of invention number: 201010512396.X) comprise reaction zone, flco growth district and non-woven membrane assembly, in non-woven membrane assembly, be provided with non-woven membrane, non-woven membrane surface and inner be provided with sewage in the Dynamic Membrane that forms of the flco that produces of colloidalmaterial cohesion.
Fluidized-bed membrane bioreactor (patent of invention number: the technique of 201010571203.8) utilizing biological fluidized bed and membrane bioreactor to combine, be the carrier granule of filling in reactor by the homemade diameter 5mm of waste Crumb rubber, gac and tackiness agent left and right, filling proportion is the 10%-30% of reactor useful volume.The synergy that relies on carrier and mud, completes biofilm, and microorganism growth, on filler, has reduced sludge concentration in reactor, changed reactor environment operational conditions and decelerating membrane pollution, thereby extended the work-ing life of film, has improved the processing power of film.
A kind of membrane bioreactor and the method for purifying waste water thereof (patent of invention number: 201010571203.8) disclose and a kind ofly divided by microorganism reactor and film filter the membrane bioreactor being arranged, extended the work-ing life of membrane bioreactor, remove the cleaning trouble of operator to film filter from, improved product water efficiency.
Although these representational bio-reactors have purified sewage to a certain extent well, have protected environment.But temperature is an important factor that affects microbial physiology activity, and under low temperature environment, too low temperature may delay or destroy the normal physiological activity of certain micro-organisms conventionally, thereby affects wastewater treatment efficiency.Therefore, can before operation, first to the microorganism on carrier, screen, select low temperature resistant microorganism and finally form microbial film, can under the condition of low temperature, purify waste water.
Meanwhile, for the river water quality of low pollution load, although patent of invention (publication number 201210014485.0) has proposed the regulate and control method of microorganism active in the membrane bioreactor of micro-polluted surface water treatment, its effect purifying water has to be tested.In addition, low pollution load water quality, it is living microorganism existence that enough nutrients may not be provided, therefore, cultivation can self-ly be survived and the microbial film group of self-recycle is the prerequisite that realizes low pollution load sewage disposal.
Summary of the invention
The object of the invention is to overcome above-mentioned shortcoming, a kind of method of utilizing natural biology membrane reactor to purify underload low temperature river is provided.This is invented, and production can self-ly be survived and the natural biology film of self-recycle, and this microbial film can tolerate low temperature, processes low temperature underload sewage.This is a kind of new design and construction pattern, and it cultivates the natural biology film consisting of autotrophy and heterotrophic microorganism, the dual purpose that purifies section, farmland low pollution load low temperature river by reaching.The present invention take that to process low pollution load river sewage winter be target, adopt the Thoughts of " subregion, classification, multistage " and " first taming natural biology film; immobilization natural biology film again; bioreactor for constructing ", according to the operational path of " water inlet-just sink-aerobic (illumination)-anaerobism (illumination)-overflow-water outlet ", design.
To achieve these goals, technical scheme of the present invention is: utilize natural biology membrane reactor to purify the method for underload low temperature river, it is characterized in that, comprise the steps:
(1) enrichment of natural biology film and cultivation: use 0.1M salt acid soak after 2 hours the artificial aquatic weed of purchasing, dry, be placed in eutrophication water, in intensity of illumination, to be greater than 3600 Lux, temperature be enrichment under 20-40 ℃ of condition, cultivate natural biology film;
(2) low temperature natural biology film domestication: will continue to cultivate at the pre-incubated natural biology film of temperature 20-40 ℃ enrichment, and reduce stage by stage culture temperature, until be reduced to culture temperature between 0-4 ℃; In the temperature range of 0-4 ℃, continue to cultivate for some time, low temperature natural biology film is regarded as and is tamed successfully, for future use;
(3) low temperature natural biology film is seeded to reactor: reactor comprises anaerobic reation pool, aerobic reaction pond, settling tank, filler, light source, aerating apparatus;
(4) natural biology membrane reactor commissioning test: natural biology membrane reactor is fixed on buoyancy aid, make reactor water inlet just as for the water surface, in aeration situation, go to river in water inflow reactor, reactor aerobic section is negative pressure, river produces and reacts with natural biology film, and pollution substance is converted, degrades, adsorbs and absorbs;
(5) natural biology membrane reactor purification of water quality: a plurality of natural biology membrane reactors are laid in river water body, river is carried out to original position purification.
Further technical scheme comprises:
In step (1), the eutrophication index of eutrophication water is greater than 50, and artificial aquatic weed and eutrophication water volume ratio are less than 1:2.
The enrichment of natural biology film and cultivation that step (1) is described, eutrophic water for enrichment and cultivation natural biology film need to contain the microorganisms such as algae, bacterium, therefore, in fact the natural biology film its " provenance " in enrichment derives from got eutrophic water.The eutrophic water that different location gathers, the natural biology membrane micro group of its cultivation forms may be different, and its removal effect to pollutent also there are differences, but the removal efficiency of the basic pollutent such as organism, nitrogen, phosphorus is more or less the same.
In step (2), reduce stage by stage the concrete mode of culture temperature as follows: it is within every two days, to reduce by 1 degree that temperature reduces gradient, until be reduced to culture temperature between 0-4 ℃, in the temperature range of 0-4 ℃, continue to cultivate one week.
The described low temperature natural biology film of step (3) is seeded to reactor, and in the anaerobic reation pool water body in reactor, dissolved oxygen content remains on below 0.2 mg/L; More than in the water body of aerobic reaction pond, dissolved oxygen content remains on 6.0 mg/L; Filler is comprised of gac, peat, sponge, fiber, non-woven fabrics, and forming weight percentage ratio is 0.1:0.5:0.1:0.2:0.1; Light source is laid in below the water surface, common incandescent watertight light fitting, consists of, and intensity of illumination is greater than 3600 Lux; Aerating apparatus consists of the pump of breeding fish (being small-sized aeration pump).
Further, natural biology film is inoculated into the concrete step of reactor and is in step (3): by enrichment the carrier of natural biology film (being aforesaid artificial aquatic weed) with scissors cut short long to 0.1-0.5 cm after, put into the filler of reactor, enrichment the access amount of carrier of natural biology film be the 0.5-5% of total filler amount.
In step (4), by regulating buoyancy aid and reactor weight, regulate hydraulic load and the residence time of reactor.
Further, the natural biology membrane reactor commissioning test that step (4) is described, when river Eutrophic Extent is lower, when eutrophication index circle is between 30 and 50, hydraulic detention time operated by rotary motion is at 12-24 hour; When river Eutrophic Extent is higher, when eutrophication index circle is between 50 and 70, hydraulic detention time operated by rotary motion is at 24-48 hour; When river Eutrophic Extent is very high, eutrophication index is greater than at 70 o'clock, and hydraulic detention time operated by rotary motion is at 48-72 hour.
The natural biology membrane reactor purification of water quality that step (5) is described, when rainy season, (be generally summer) takes out natural biology membrane reactor from river course, be placed on indoor shady and cool part (temperature is lower than 15 ℃), active to guarantee natural biology membrane micro, while avoiding next use in winter natural biology membrane reactor purifying river water, need acclimated microorganism again.Meanwhile, take out natural biology film rainy season, is also beneficial to river course flood discharge, irrigation and drainage.
Further, in step (5), every 100 square meter rivers are laid a natural biology membrane reactor.
Winter, river was under natural biology membrane reactor original position purifies, nutritive substance in river water body constantly reduces, organic pollution load reaches continuous decomposition, degraded, the heavy metal contaminants of the low pollution load of part is also removed or is transformed, and has finally reached the object of winter low temperature riverway water quality purification.
Technique effect following (average results that sampling in winter is 6 times) before and after the invention process: before enforcement, contain a large amount of total suspended matter (TSS), organism in river water body (with COD and UV 254nm-matter ultraviolet absorptivity represents), nitrogen and phosphorus content and a small amount of heavy metal be as Hg, Cr, Cu, Cd, after natural biology membrane reactor application of the present invention, these pollution substances are purified substantially, pollutent reduction rate reaches 75% above (see figure 2).
The inventive method simply, is easily gone, and purifies in the winter time river water quality effective.Compare with traditional riverway water quality purification technology (as artificial floating bed, add chemical agent etc.), method of the present invention has effect stability, processing efficiency and is not subject to low temperature effect, construction cost is low, running cost is low feature.The natural biology film of invention has once the feature that drops into, uses for many years, and the natural biology film of domestication can life-time service, and the similar microbial population of multipliability matter, further lowers running cost.In addition, during the application of natural biology membrane reactor, can not affect normal flood discharge and the irrigation and drainage in river course, be convenient to promote.
Accompanying drawing explanation
Fig. 1 is the structure iron of natural biology membrane reactor;
Fig. 2 is that natural biology film of the present invention purifies river sewage design sketch winter;
Wherein, 1 settling tank, 2 anaerobic reation pools, 3 aerobic reaction ponds, 4 settling tanks, 5 water outlets, 6 fillers, 7 light sources, 8 aerating apparatuss.
Embodiment
Below in conjunction with Fig. 1 and Fig. 2 in detail technical scheme of the present invention is described in detail.
Utilize natural biology membrane reactor to purify the method for underload low temperature river, it is characterized in that, comprise the steps:
(1) enrichment of natural biology film and cultivation: use 0.1M salt acid soak after 2 hours the artificial aquatic weed of purchasing, dry, be placed in eutrophication water, in intensity of illumination, to be greater than 3600 Lux, temperature be enrichment under 20-40 ℃ of condition, cultivate natural biology film;
(2) low temperature natural biology film domestication: will continue to cultivate at the pre-incubated natural biology film of temperature 20-40 ℃ enrichment, and reduce stage by stage culture temperature, until be reduced to culture temperature between 0-4 ℃; In the temperature range of 0-4 ℃, continue to cultivate for some time, low temperature natural biology film is regarded as and is tamed successfully, for future use;
(3) low temperature natural biology film is seeded to reactor: reactor comprises settling tank 1, anaerobic reation pool 2, aerobic reaction pond 3, settling tank 4, water outlet 5, filler 6, light source 7, aerating apparatus 8;
(4) natural biology membrane reactor commissioning test: natural biology membrane reactor is fixed on buoyancy aid, make reactor water inlet just as for the water surface, in aeration situation, go to river in water inflow reactor, reactor aerobic section is negative pressure, river produces and reacts with natural biology film, and pollution substance is converted, degrades, adsorbs and absorbs;
(5) natural biology membrane reactor purification of water quality: a plurality of natural biology membrane reactors are laid in river water body, river is carried out to original position purification.
Embodiment 1: a lake is a typical eutrophic lake near the bridge of oil mill, Nanjing, to gather the river enrichment of returning in its ingoing river and to cultivate natural biology film, take the carrier of artificial aquatic weed as natural biology film.Natural biology film enrichment and cultivating after 35 days, starts to reduce culture temperature within every 2 days, to reduce the gradient of 1 degree, until after culture temperature is 4 ℃, continue to cultivate 7 days at this temperature.Bio-reactor comprises settling tank, anaerobic reation pool, aerobic reaction pond, settling tank, hand-stuff, and the shared reactor cumulative volume ratio of above each several part is 10%, 30%, 30%, 10%, 20%.Hand-stuff is comprised of gac, peat, sponge, fiber, non-woven fabrics, and formation part by weight is 0.1:0.5:0.1:0.2:0.1.Utilizing common LED water proof lamp is light source, utilizes the common pump of breeding fish for aerator pump (power 3.2W).With scissors, the carrier that has adhered to natural biology film is sheared, inoculated in the filler of bio-reactor, inoculum size is about 1% of total filler amount.Because experimental session river course eutrophication index circle is between 30 and 50, therefore, by regulating the size of buoyancy aid, setting natural biology membrane reactor hydraulic detention time is 24 hours.
By scheduling to last bimestrial winter, test, result is presented at the application of natural biology membrane reactor, can purify preferably river sewage, removes underload pollution substance.TSS, COD, UV254nm-matter, TN, TP, Hg, Cr, the average removal rate of Cu and Cd is respectively 89.3%, 75.4%, and 80.3%, 81.2%, 79.6%, 84.4%, 80.6%, 83.2% and 89.3%.
Embodiment 2: Kunming Daqinghe River is the main ingoing river of Dian Chi, and both sides, river course are leek and fresh flower plantation agriculture district, and river water quality is in serious eutrophic state, and eutrophication index is greater than 70, the heavy metal contaminants that contains a small amount of underload.For purifying this sewage, indoor, carried out experimental study, will gather the river of returning and be placed in the water vat of 1.2 cubic metres, the natural biology membrane reactor of embodiment 1 purifies, the removal efficiency of each pollutent is respectively TSS 87.2%, COD 82.5%, and UV254nm-matter 79.1%, and TN 67.4%, TP 71.2%, Hg 80.6%, and Cr 79.4%, Cu83.3% and Cd 85.2%.In a word, after natural biology membrane reactor of the present invention is implemented, winter, river sewage was significantly purified, and had solved the low problem of low temperature contaminant removal efficiency.
Above-described embodiment does not limit the present invention in any form, and all employings are equal to replaces or technical scheme that the mode of equivalent transformation obtains, all drops on protection scope of the present invention.

Claims (8)

1. utilize natural biology membrane reactor to purify the method for underload low temperature river, it is characterized in that, comprise the steps:
(1) enrichment of natural biology film and cultivation: the artificial aquatic weed purchased, is dried after 2 hours by 0.1M salt acid soak, be placed in eutrophication water, in intensity of illumination, to be greater than 3600 Lux, temperature be enrichment under 20-40 ℃ of condition, cultivate natural biology film;
(2) low temperature natural biology film domestication: will continue to cultivate at the pre-incubated natural biology film of temperature 20-40 ℃ enrichment, and reduce stage by stage culture temperature, until be reduced to culture temperature between 0-4 ℃; In the temperature range of 0-4 ℃, continue to cultivate for some time, low temperature natural biology film is regarded as and is tamed successfully, for future use;
(3) low temperature natural biology film is seeded to reactor: reactor comprises anaerobic reation pool, aerobic reaction pond, settling tank, filler, light source, aerating apparatus;
(4) natural biology membrane reactor commissioning test: natural biology membrane reactor is fixed on buoyancy aid, make reactor water inlet just as for the water surface, in aeration situation, go to river in water inflow reactor, reactor aerobic section is negative pressure, river produces and reacts with natural biology film, and pollution substance is converted, degrades, adsorbs and absorbs;
(5) natural biology membrane reactor purification of water quality: a plurality of natural biology membrane reactors are laid in river water body, river is carried out to original position purification;
The described low temperature natural biology film of step (3) is seeded to reactor, and in the anaerobic reation pool water body in reactor, dissolved oxygen content remains on below 0.2 mg/L; More than in the water body of aerobic reaction pond, dissolved oxygen content remains on 6.0 mg/L; Filler is comprised of gac, peat, sponge, fiber, non-woven fabrics, and forming weight percentage ratio is 0.1:0.5:0.1:0.2:0.1; Light source is laid in below the water surface, and intensity of illumination is greater than 3600 Lux; Aerating apparatus consists of the pump of breeding fish.
2. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 1, it is characterized in that, natural biology film is inoculated into the concrete step of reactor and is in step (3): by enrichment the carrier of natural biology film cut short long to 0.1-0.5 cm after, put into the filler of reactor, access amount is the 0.5-5% of total filler amount.
3. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 1, is characterized in that, in step (4), by regulating buoyancy aid and reactor weight, regulates hydraulic load and the residence time of reactor.
4. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 3, it is characterized in that, the natural biology membrane reactor commissioning test concrete steps of step (4) are: when river eutrophication index circle is between 30 and 50, hydraulic detention time is set to 12-24 hour; When river eutrophication index circle is between 50 and 70, hydraulic detention time is set to 24-48 hour; When river eutrophication index is greater than 70, hydraulic detention time is set to 48-72 hour.
5. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 1, it is characterized in that, the described natural biology membrane reactor purification of water quality concrete steps of step (5) are: when rainy season or summer, natural biology membrane reactor is taken out from river course, be placed on temperature lower than the indoor shady and cool part of 15 ℃, active to guarantee natural biology membrane micro, while avoiding next use in winter natural biology membrane reactor purifying river water, need acclimated microorganism again.
6. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 1, it is characterized in that, in step (1), the eutrophication index of eutrophication water is greater than 50, artificial aquatic weed and eutrophication water volume ratio are less than 1:2, and eutrophication water contains algea and bacteria.
7. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 1, it is characterized in that, in step (2), reduce stage by stage the concrete mode of culture temperature as follows: it is within every two days, to reduce by 1 degree that temperature reduces gradient, until be reduced to culture temperature between 0-4 ℃, in the temperature range of 0-4 ℃, continue to cultivate one week.
8. the method for utilizing natural biology membrane reactor to purify underload low temperature river according to claim 1, is characterized in that, in step (5), every 100 square meter rivers are laid a natural biology membrane reactor.
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