CN103239498A - Traditional chinese medicine extract - Google Patents
Traditional chinese medicine extract Download PDFInfo
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- CN103239498A CN103239498A CN2013101721722A CN201310172172A CN103239498A CN 103239498 A CN103239498 A CN 103239498A CN 2013101721722 A CN2013101721722 A CN 2013101721722A CN 201310172172 A CN201310172172 A CN 201310172172A CN 103239498 A CN103239498 A CN 103239498A
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Abstract
The invention relates to a natural medicine composition, a preparation method thereof and application of the composition to preparation of medicines for reducing toxicity and enhancing efficacy in cancer chemotherapy. The natural medicine composition is prepared from astragalus and pomegranate rind water decocted and concentrated extract, astragalus polysaccharides, pomegranate rind ethanol extract and pomegranate rind tannin in a ratio of (2:7):(2:6):(3-5):(5-7). The composition has excellent curative effects on cancer chemotherapy-induced diarrhea, and can simultaneously have a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.The chemotherapy prescription with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and its etiology and pathogenesis mainly is the deficient of body negative and positive of qi and blood due to the spleen injury.Chinese medicine attracts people's attention for the research of having loose bowels, and present unmatchful anticancer disease chemotherapy still has the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
The Radix Astragali is the root of leguminous herbaceous plant's Radix Astagali, Radix Astragali, has invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus effect.Be used for the chronic diarrhea proctoptosis.Pericarpium Granati is the dry peel of Punicaceae plant Punica granatum L., and the energy relieving diarrhea with astringents cures mainly chronic diarrhea, chronic dysentery.In recent years, the Radix Astragali and Pericarpium Granati antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, it is the compositions of novelty, and proved that it has fabulous curative effect and can finish the present invention thus simultaneously to chemotherapeutic sensitivity cancer chemotherapy dependency diarrhoea.
Summary of the invention
A kind of natural drug composition provided by the invention, by Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin by weight in 2-7: the ratio of 2-6: 3-5: 5-7 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin is 7: 6: 5: 7.
Wherein, Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Radix Astragali Pericarpium Granati water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got the Radix Astragali, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
The preparation method of astragalus polysaccharides is in the above-mentioned natural drug composition:
The Radix Astragali is pulverized, and with drying behind the 95% ethanol extraction saponin, adds distilled water, dropwise add saturated limewater, adjust pH leaves standstill 10min with the moistening material, microwave radiation 1min, radiation 1min again behind the 1min at interval, filter extracting solution, repeat aforesaid operations 2-3 time, merge extractive liquid, concentrates, regulate pH=7 with 10% hydrochloric acid, add 95% ethanol, wash out polysaccharide, filter, washing with alcohol is drying to obtain astragalus polysaccharides.
Pericarpium Granati ethanol extraction preparation method is in the above-mentioned natural drug composition:
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
Pericarpium Granati tannin preparation method is in the above-mentioned natural drug composition:
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, the rotary evaporation desolventizing, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization is namely.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Radix Astragali Pericarpium Granati water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got the Radix Astragali, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
(2) preparation of astragalus polysaccharides
The Radix Astragali is pulverized, and with drying behind the 95% ethanol extraction saponin, adds distilled water, dropwise add saturated limewater, adjust pH leaves standstill 10min with the moistening material, microwave radiation 1min, radiation 1min again behind the 1min at interval, filter extracting solution, repeat aforesaid operations 2-3 time, merge extractive liquid, concentrates, regulate pH=7 with 10% hydrochloric acid, add 95% ethanol, wash out polysaccharide, filter, washing with alcohol is drying to obtain.
(3) Pericarpium Granati ethanol extraction preparation
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
(4) Pericarpium Granati tannin preparation
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, the rotary evaporation desolventizing, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization is namely.
(5) choose Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin in proportion, evenly mix, namely.
The preparation method of above-mentioned natural drug composition also comprises: the product that step (5) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition performance synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-7: 2-6: 3-5: produce synergism during 5-7, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 7: 6: 5: 7 o'clock especially obvious, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and each component of Pericarpium Granati tannin all can obtain by conventional means, wherein component such as astragalus polysaccharides also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Radix Astragali Pericarpium Granati water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got the Radix Astragali, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
(2) preparation of astragalus polysaccharides
The Radix Astragali is pulverized, and with drying behind the 95% ethanol extraction saponin, adds distilled water, dropwise add saturated limewater, adjust pH leaves standstill 10min with the moistening material, microwave radiation 1min, radiation 1min again behind the 1min at interval, filter extracting solution, repeat aforesaid operations 2-3 time, merge extractive liquid, concentrates, regulate pH=7 with 10% hydrochloric acid, add 95% ethanol, wash out polysaccharide, filter, washing with alcohol is drying to obtain.
(3) Pericarpium Granati ethanol extraction preparation
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
(4) Pericarpium Granati tannin preparation
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, the rotary evaporation desolventizing, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization is namely.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Radix Astragali Pericarpium Granati water boiling concentration extract 28mg, astragalus polysaccharides 24mg, Pericarpium Granati ethanol extraction 20mg and the Pericarpium Granati tannin 28mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Radix Astragali Pericarpium Granati water boiling concentration extract 28mg, astragalus polysaccharides 24mg, Pericarpium Granati ethanol extraction 20mg and the Pericarpium Granati tannin 28mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin is 7: 6: 5: 7.The inventor to above-mentioned each composition usage ratio at 2-7: 2-6: 3-5: the compositions that the multiple proportioning combination in the time of between the 5-7 obtains has been carried out and last identical experiment, find that all natural drug composition can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Radix Astragali, Pericarpium Granati is purchased in medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Radix Astragali Pericarpium Granati water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is astragalus polysaccharides 10g.kg
-1(being equivalent to contain the crude drug amount), D group Pericarpium Granati ethanol extraction and Pericarpium Granati tannin were by 5: 7 mixed 10g.kg
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, the degree of Radix Astragali Pericarpium Granati water boiling concentration extract group chemotherapy dependency diarrhoea is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin by weight in 2-7: the ratio of 2-6: 3-5: 5-7 is formed
Wherein Radix Astragali Pericarpium Granati water boiling concentration preparation method of extract is:
Be 1-4 according to proportion compatibility: 1 gets the Radix Astragali, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely;
Wherein the preparation method of astragalus polysaccharides is:
The Radix Astragali is pulverized, and with drying behind the 95% ethanol extraction saponin, adds distilled water, dropwise add saturated limewater, adjust pH leaves standstill 10min with the moistening material, microwave radiation 1min, radiation 1min again behind the 1min at interval, filter extracting solution, repeat aforesaid operations 2-3 time, merge extractive liquid, concentrates, regulate pH=7 with 10% hydrochloric acid, add 95% ethanol, wash out polysaccharide, filter, washing with alcohol, namely dry;
Wherein the preparation method of Pericarpium Granati ethanol extraction is:
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, gets light yellow to the yellowish-brown powder, namely after the extractum lyophilization;
Wherein the preparation method of Pericarpium Granati tannin is:
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, the rotary evaporation desolventizing, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization is namely.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin is 7: 6: 5: 7.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Radix Astragali Pericarpium Granati water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets the Radix Astragali, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely;
(2) preparation of astragalus polysaccharides
The Radix Astragali is pulverized, and with drying behind the 95% ethanol extraction saponin, adds distilled water, dropwise add saturated limewater, adjust pH leaves standstill 10min with the moistening material, microwave radiation 1min, radiation 1min again behind the 1min at interval, filter extracting solution, repeat aforesaid operations 2-3 time, merge extractive liquid, concentrates, regulate pH=7 with 10% hydrochloric acid, add 95% ethanol, wash out polysaccharide, filter, washing with alcohol is drying to obtain;
(3) preparation of Pericarpium Granati ethanol extraction
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, gets light yellow to the yellowish-brown powder, namely after the extractum lyophilization;
(4) preparation of Pericarpium Granati tannin
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, the rotary evaporation desolventizing, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization is namely;
(5) choose Radix Astragali Pericarpium Granati water boiling concentration extract, astragalus polysaccharides, Pericarpium Granati ethanol extraction in proportion and evenly mix with the Pericarpium Granati tannin, namely.
4. the preparation method of natural drug composition as claimed in claim 3, it is characterized in that also comprising: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101428047A (en) * | 2008-12-16 | 2009-05-13 | 中国科学院新疆理化技术研究所 | Process for producing pericarpium granati total phenols and uses thereof |
| CN102293830A (en) * | 2011-09-05 | 2011-12-28 | 胶南市妇幼保健院 | Navel-applying Chinese medicine for treating infantile chronic diarrhea |
| CN103230465A (en) * | 2013-04-08 | 2013-08-07 | 张宗升 | Traditional chinese medicine extract |
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2013
- 2013-04-16 CN CN2013101721722A patent/CN103239498A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101428047A (en) * | 2008-12-16 | 2009-05-13 | 中国科学院新疆理化技术研究所 | Process for producing pericarpium granati total phenols and uses thereof |
| CN102293830A (en) * | 2011-09-05 | 2011-12-28 | 胶南市妇幼保健院 | Navel-applying Chinese medicine for treating infantile chronic diarrhea |
| CN103230465A (en) * | 2013-04-08 | 2013-08-07 | 张宗升 | Traditional chinese medicine extract |
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Application publication date: 20130814 |