CN103238516A - Factorial seedling culturing method of blueberries - Google Patents
Factorial seedling culturing method of blueberries Download PDFInfo
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- CN103238516A CN103238516A CN 201210023578 CN201210023578A CN103238516A CN 103238516 A CN103238516 A CN 103238516A CN 201210023578 CN201210023578 CN 201210023578 CN 201210023578 A CN201210023578 A CN 201210023578A CN 103238516 A CN103238516 A CN 103238516A
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Abstract
The invention discloses a factorial seedling culturing method of blueberries, relates to a traditional Chinese medicinal material cultivating technology, and belongs to the technical field of the seedling culturing of the blueberries. Indoleacetic acid, LFS, agar, sucrose and a lemon juice are added to an MS culturing solution which is a base to obtain a nutrient solution suitable for the growth of the blueberries in order to carry out the large-scale factorial seedling culture. According to the invention, applied raw materials are less, and the product quantity is large, so the breeding coefficient is great increased; blueberry seedlings grow under an aseptic condition, so insect disease harms are small, seedlings grow strong, and the quality is high; and the region restriction is broken, so the blueberries can smoothly grow in the northern area.
Description
Technical field
The present invention relates to traditional Chinese medicine culture technique aspect, belong to the seedling growing process field of blueberry, concrete is a kind of factorial seedling-culturing method of blueberry.
Background technology
Blueberry is little berry, and it is blue that fruit is, subcircular, and the pulp exquisiteness, seed is minimum, sweet acid appropriateness, and have fragrant refreshing pleasant fragrance, and can eat raw, also can be used as the raw material of food, beverage, medicine etc.Be rich in multiple nutritional components common in the fruit in the blueberry fruit, and antioxidant and bacterial growth inhibitor such as anthocyanin, flavonoids, the fruit that belongs to homoamino acid, high zinc, high ferro, high-copper, homovitamin, has good nutrition health-care functions, comprise strengthen eyesight, anti-eye strain, prevent that cerebral nerve is aging, anticancer, softening blood vessel, enhancing human immunity etc., its nutritive value can be rated as " king of world's fruit " far above fruit such as apple, grape, oranges.At present, domestic northern area is seldom seen blueberry growth, and it introduce a fine variety, breeding of new variety, breeding and popularization aspect are lagged behind, and the support of corresponding scientific theory can't be provided.Therefore, seek a kind of blueberry that makes in the northern area smooth growth, fast the breeding growth technology extremely important.
Summary of the invention
The object of the present invention is to provide a kind of factorial seedling-culturing method of blueberry, solved the problem that blueberry emergence rate in the cultivated in north process is low, damage by disease and insect serious, be difficult for surviving.
To achieve these goals, the present invention adopts a kind of factorial seedling-culturing method of blueberry, finishes by following steps:
1, explant is prepared: adopting blueberry tender stem segments in spring is explant material, aseptic water washing 15-20 minute, and on clean workbench, the alcohol disinfecting 6-8 second with 70%, aseptic water washing 3 times, each 3-5 minute;
2, induce cultivation: under the aseptic condition, explant material is sheared, put into inducing culture and cultivate, condition of culture is 25 ± 2 ℃ of room temperatures, intensity of illumination 2500-3000lx, and light application time is 12-16 hour every day, all the other time dark processing, continuous culture 12-14 days;
3, propagation is cultivated: will put into aseptic proliferated culture medium through the seedling of inducing cultivation to obtain, condition of culture is for cultivating 26 ± 2 ℃ of the temperature inside the box, intensity of illumination 3000-4000lx, light application time 14-16 every day hour, all the other time dark processing, incubation time are 20-30 days;
4, culture of rootage: cut the high blueberry seedling of 2-3cm, put into the sterile rootage medium, carry out culture of rootage, training buckwheat condition is for cultivating 29 ± 1 ℃ of the temperature inside the box, intensity of illumination 3000-5000lx, light application time 16-18 every day hour, all the other time dark processing, rootage duration is 30-40 days, acclimatization and transplants behind the blueberry seedling root 0.6-0.8cm;
Bud inducing culture described in the step 2 of the present invention is: MS+0.2-0.5mg/L heteroauxin+0.1-0.5mg/L spirit hair element+600-1000mg/L agar+500-600mg/L sucrose+200-500mg/L lemon juice, and the pH value is 5.8-6.1;
Proliferated culture medium described in the step 3 of the present invention is: 1/2MS+0.3-0.5mg/L heteroauxin+0.2-0.3mg/L spirit hair element+500-600mg/L agar+300-500mg/L sucrose+200-500mg/L lemon juice, and the pH value is 5.8-6.1;
Root media of the present invention is: 1/2MS+0.2-0.5mg/L heteroauxin+600-1000mg/L agar+200-500mg/L lemon juice, the pH value is 5.8-6.1.
The factorial seedling-culturing method of a kind of blueberry of the present invention, its beneficial effect is: use raw material few, product quantity is big, increases substantially reproduction coefficient; The blueberry seedling all grows under aseptic condition, and damage by disease and insect harm is little, growth of seedling stalwartness, quality height; Breaking the region restriction, is that blueberry can be in the northern area smooth growth.
Embodiment
The factorial seedling-culturing method of a kind of blueberry of the present invention specifies by following example.
Example 1
1, explant is prepared: adopting blueberry tender stem segments in spring is explant material, aseptic water washing 15 minutes, and on clean workbench, the alcohol disinfecting with 70% 6 seconds, aseptic water washing 3 times, each 3-5 minute;
2, induce cultivation: under the aseptic condition, explant material is sheared, put into inducing culture and cultivate, filling a prescription is: MS+0.2mg/L heteroauxin+0.2mg/L spirit hair element+800mg/L agar+500mg/L sucrose+400mg/L lemon juice, and the pH value is 5.8; Condition of culture is 26 ℃ of room temperatures, intensity of illumination 2500-3000lx, and light application time is 12-16 hour every day, all the other time dark processing, continuous culture 12-14 days;
3, propagation is cultivated: will put into aseptic proliferated culture medium through the seedling of inducing cultivation to obtain, filling a prescription is: 1/2MS+0.3mg/L heteroauxin+0.2mg/L spirit hair element+500mg/L agar+300mg/L sucrose+200mg/L lemon juice, and the pH value is 5.8; Condition of culture is 27 ℃ of cultivation the temperature inside the box, intensity of illumination 3000-4000lx, and light application time 14-16 every day hour, all the other time dark processing, incubation time is 20-30 days;
4, culture of rootage: cut the high blueberry seedling of 2-3cm, put into the sterile rootage medium, carry out culture of rootage, filling a prescription is: 1/2MS+0.2mg/L heteroauxin+600mg/L agar+200mg/L lemon juice, and the pH value is 5.8; Training buckwheat condition is 29 ℃ of cultivation the temperature inside the box, intensity of illumination 3000-5000lx, and light application time 16-18 every day hour, all the other time dark processing, rootage duration is 30-40 days;
5, hardening: hardening behind the blueberry seedling root 0.6-0.8cm, hardening matrix are 1: 2: 1 vermiculite, peat and perlite, and the hardening temperature is 30 ℃, and all the other conditions are identical with the culture of rootage condition, and hardening is transplanted the land for growing field crops after one week.
Example 2
1, explant is prepared: adopting blueberry tender stem segments in spring is explant material, aseptic water washing 20 minutes, and on clean workbench, the alcohol disinfecting with 70% 8 seconds, aseptic water washing 3 times, each 3-5 minute;
2, induce cultivation: under the aseptic condition, explant material is sheared, put into inducing culture and cultivate, filling a prescription is: MS+0.5mg/L heteroauxin+0.5mg/L spirit hair element+1000mg/L agar+600mg/L sucrose+500mg/L lemon juice, and the pH value is 6.1; Condition of culture is 27 ℃ of room temperatures, intensity of illumination 2500-3000lx, and light application time is 12-16 hour every day, all the other time dark processing, continuous culture 12-14 days;
3, propagation is cultivated: will put into aseptic proliferated culture medium through the seedling of inducing cultivation to obtain, filling a prescription is: 1/2MS+0.5mg/L heteroauxin+0.3mg/L spirit hair element+600mg/L agar+500mg/L sucrose+500mg/L lemon juice, and the pH value is 6.1; Condition of culture is 28 ℃ of cultivation the temperature inside the box, intensity of illumination 3000-4000lx, and light application time 14-16 every day hour, all the other time dark processing, incubation time is 20-30 days;
4, culture of rootage: cut the high blueberry seedling of 2-3cm, put into the sterile rootage medium, carry out culture of rootage, filling a prescription is: 1/2MS+0.5mg/L heteroauxin+1000mg/L agar+500mg/L lemon juice, and the pH value is 6.1; Training buckwheat condition is 29 ℃ of cultivation the temperature inside the box, intensity of illumination 3000-5000lx, and light application time 16-18 every day hour, all the other time dark processing, rootage duration is 30-40 days;
5, hardening: hardening behind the blueberry seedling root 0.6-0.8cm, hardening matrix are 1: 2: 1 vermiculite, peat and perlite, and the hardening temperature is 31 ℃, and all the other conditions are identical with the culture of rootage condition, and hardening is transplanted the land for growing field crops after one week.
Claims (2)
1. the factorial seedling-culturing method of a blueberry, comprise and induce cultivation, propagation to cultivate and culture of rootage, it is characterized in that: the inducing culture prescription is MS+0.2-0.5mg/L heteroauxin+0.1-0.5mg/L spirit hair element+600-1000mg/L agar+500-600mg/L sucrose+200-500mg/L lemon juice; The proliferated culture medium prescription is: 1/2MS+0.3-0.5mg/L heteroauxin+0.2-0.3mg/L spirit hair element+500-600mg/L agar+300-500mg/L sucrose+200-500mg/L lemon juice; The culture of rootage based formulas is: 1/2MS+0.2-0.5mg/L heteroauxin+600-1000mg/L agar+200-500mg/L lemon juice.
2. the factorial seedling-culturing method of a kind of blueberry according to claim 1 is characterized in that: induce cultivation, propagation to cultivate and the pH of culture of rootage is 5.8-6.1.
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| CN 201210023578 CN103238516A (en) | 2012-02-02 | 2012-02-02 | Factorial seedling culturing method of blueberries |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885773A (en) * | 2015-06-29 | 2015-09-09 | 云南省农业科学院花卉研究所 | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries |
| CN104026012B (en) * | 2014-06-09 | 2016-05-04 | 赵兰 | A kind of cowberry strengthening seedling and rooting culture medium |
| CN114073226A (en) * | 2021-11-23 | 2022-02-22 | 河北农业大学 | Inoculation method for tissue culture and rapid propagation of raspberry petioles |
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2012
- 2012-02-02 CN CN 201210023578 patent/CN103238516A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026012B (en) * | 2014-06-09 | 2016-05-04 | 赵兰 | A kind of cowberry strengthening seedling and rooting culture medium |
| CN104885773A (en) * | 2015-06-29 | 2015-09-09 | 云南省农业科学院花卉研究所 | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries |
| CN104885773B (en) * | 2015-06-29 | 2017-03-15 | 云南省农业科学院花卉研究所 | A kind of method of quickly breeding blue berry early stage sizing tissue culture commercial seedling |
| CN114073226A (en) * | 2021-11-23 | 2022-02-22 | 河北农业大学 | Inoculation method for tissue culture and rapid propagation of raspberry petioles |
| CN114073226B (en) * | 2021-11-23 | 2023-10-27 | 河北农业大学 | Inoculation method for tissue culture and rapid propagation of raspberry leaf stalks |
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Application publication date: 20130814 |